Rroxscaffold_5G00359210

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0015806
Physical Location & Seq
Forward (+)
39291526 .. 39293829
2304 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00359210.1

Sequence Viewer

Length: 846 bp
ATGCCATTTTCTTCTTCCATGGTCTATAAGTTCTACGGCCAAAAGAAATTGGCTACTTACACCATCACCGTAGATCAATCCGGCCAAGGAAACTTCACCAAAATACAATCTGCCATCGATGCTGTTCCTTCAAACAACAAGCATTGGGTTTGCATCAAGATCTCCGCCGGCACCTATAGGGAGAAGGTGAAAATCCCTTTTGATAAGCCGTACATACTTCTGAAAGGTGAAAACAAGCGACAAACACAAATTGTTTGGGATGATATTGGCCCTGTTGACCAAAGTGCCACTTTCACTTCTGAAGCCCTTAGTATCACTGTCAAATCCATCACTTTTGCTACACTTTGTGGGCGGTTGAAGGACGCCACTTTTACAAGCATTGCACTATCCCAAGGTGCTATTGATTTTATCTTTGGCCATGCCCAGGCCATTTTCCAGGGATGCTCCATATCAGTGCTTGCGGGAAATCTGCAGCCCGGTGGGATAGGATACATCACAGCACAGGGCAGAGAACATCCAAATGAACCTGGTGCTTTTATTTTCAAGAATTGCAGGGTGCATGGTACTGGTTTAACCTATTTGGGAAGGGCATGGAGACTTTACTCGAGGGTCATTTTCTATGATTCGAACTTATCAGACATTGTAGTCCCTCAGGGTTGGGATGTTTGGCAGGCTCTTGGCCATGAGGACCAATTGACATTTGCCGAGTATGGGTGCTATGGTCCAGGAGCCGACACTTCGAAGCGTGTGAGTTGGGAAAAGAAGCTTAGCCCTAATATCGTGAAACAGTTTACTAGCTTGAATTTCATTGATTCTGATGGATGGCTCATTGACCAGCCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

281

Amino Acids

31.19

Weight (kDa)

8.49

Isoelectric Point (pI)

31.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 22 - 119 1.9e-13 Pectinesterase
Pectinesterase PF01095 131 - 272 2.2e-34 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015806)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24130 AT5G18990
fragaria_vesca FvH4_4g15880
malus_domestica MD04G1008100.v1.1
prunus_persica Prupe.1G006800_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0415461
rosa_laevigata RLG00000008069
rosa_multiflora Rmu_sc0004076.1_g000023
rosa_roxburghii Rroxscaffold_5G00359210
rosa_rugosa Rorug04G0134400
rosa_samantha Rh4AG194800 Rh4BG193500 Rh4CG205800 Rh4DG192500
rosa_wichuraiana Rw4G016590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 644
AccB1I GGYRCC 1 cut(s) 170
AciI CCGC 3 cut(s) 165, 352, 461
AcoI YGGCCR 4 cut(s) 37, 82, 415, 679
AcsI RAATTY 1 cut(s) 802
AcuI CTGAAG 1 cut(s) 321
AcyI GRCGYC 1 cut(s) 363
AdeI CACNNNGTG 1 cut(s) 347
AfaI GTAC 2 cut(s) 212, 565
AfiI CCNNNNNNNGG 2 cut(s) 424, 711
AgsI TTSAA 4 cut(s) 132, 358, 544, 802
AjnI CCWGG 4 cut(s) 423, 435, 526, 724
AluBI AGCT 2 cut(s) 766, 798
AluI AGCT 2 cut(s) 766, 798
Alw26I GTCTC 1 cut(s) 589
Ama87I CYCGRG 1 cut(s) 604
AoxI GGCC 6 cut(s) 37, 82, 268, 415, 426, 679
ApeKI GCWGC 1 cut(s) 472
ApoI RAATTY 1 cut(s) 802
AspS9I GGNCC 3 cut(s) 269, 688, 722
AsuC2I CCSGG 1 cut(s) 477
AsuHPI GGTGA 4 cut(s) 58, 88, 199, 239
AsuII TTCGAA 2 cut(s) 626, 740
AvaI CYCGRG 1 cut(s) 604
AvaII GGWCC 2 cut(s) 688, 722
AxyI CCTNAGG 1 cut(s) 651
BalI TGGCCA 2 cut(s) 417, 681
BanI GGYRCC 1 cut(s) 170
BbvI GCAGC 1 cut(s) 484
BccI CCATC 5 cut(s) 71, 122, 335, 812, 816
BceAI ACGGC 2 cut(s) 52, 193
BciT130I CCWGG 4 cut(s) 425, 437, 528, 726
BciVI GTATCC 1 cut(s) 482
BcnI CCSGG 1 cut(s) 477
BcoDI GTCTC 1 cut(s) 589
BfaI CTAG 1 cut(s) 795
BfmI CTRYAG 2 cut(s) 175, 470
BfuI GTATCC 1 cut(s) 482
BglII AGATCT 1 cut(s) 159
BisI GCNGC 1 cut(s) 473
BlpI GCTNAGC 1 cut(s) 767
BlsI GCNGC 1 cut(s) 474
Bme1390I CCNGG 5 cut(s) 425, 437, 477, 528, 726
Bme18I GGWCC 2 cut(s) 688, 722
BmeT110I CYCGRG 1 cut(s) 604
BmgT120I GGNCC 3 cut(s) 269, 688, 722
BmiI GGNNCC 2 cut(s) 172, 730
BmrFI CCNGG 5 cut(s) 425, 437, 477, 528, 726
BmsI GCATC 3 cut(s) 109, 162, 431
Bpu1102I GCTNAGC 1 cut(s) 767
Bpu14I TTCGAA 2 cut(s) 626, 740
BpuMI CCSGG 1 cut(s) 477
Bsa29I ATCGAT 1 cut(s) 117
BsaHI GRCGYC 1 cut(s) 363
BsaJI CCNNGG 5 cut(s) 18, 85, 391, 423, 436
Bsc4I CCNNNNNNNGG 2 cut(s) 424, 711
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 1 cut(s) 571
Bse21I CCTNAGG 1 cut(s) 651
Bse3DI GCAATG 1 cut(s) 378
BseBI CCWGG 4 cut(s) 425, 437, 528, 726
BseCI ATCGAT 1 cut(s) 117
BseDI CCNNGG 5 cut(s) 18, 85, 391, 423, 436
BseGI GGATG 5 cut(s) 265, 446, 514, 667, 827
BseLI CCNNNNNNNGG 2 cut(s) 424, 711
BseMI GCAATG 1 cut(s) 378
BseMII CTCAG 1 cut(s) 665
BseNI ACTGG 1 cut(s) 571
BseXI GCAGC 1 cut(s) 484
BshFI GGCC 6 cut(s) 39, 84, 270, 417, 428, 681
BshNI GGYRCC 1 cut(s) 170
BshVI ATCGAT 1 cut(s) 117
BsiHKCI CYCGRG 1 cut(s) 604
BsiSI CCGG 3 cut(s) 81, 168, 477
BslFI GGGAC 1 cut(s) 632
BslI CCNNNNNNNGG 2 cut(s) 424, 711
BsmAI GTCTC 1 cut(s) 589
BsmFI GGGAC 1 cut(s) 632
BsnI GGCC 6 cut(s) 39, 84, 270, 417, 428, 681
BsoBI CYCGRG 1 cut(s) 604
Bsp119I TTCGAA 2 cut(s) 626, 740
Bsp143I GATC 2 cut(s) 73, 159
Bsp1720I GCTNAGC 1 cut(s) 767
Bsp19I CCATGG 1 cut(s) 18
BspACI CCGC 3 cut(s) 165, 352, 461
BspANI GGCC 6 cut(s) 39, 84, 270, 417, 428, 681
BspCNI CTCAG 1 cut(s) 664
BspDI ATCGAT 1 cut(s) 117
BspLI GGNNCC 2 cut(s) 172, 730
BspMAI CTGCAG 1 cut(s) 474
BspT104I TTCGAA 2 cut(s) 626, 740
BspT107I GGYRCC 1 cut(s) 170
BsrDI GCAATG 1 cut(s) 378
BsrFI RCCGGY 1 cut(s) 167
BsrI ACTGG 1 cut(s) 571
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 5 cut(s) 18, 85, 391, 423, 436
BssMI GATC 2 cut(s) 73, 159
BssNI GRCGYC 1 cut(s) 363
BssT1I CCWWGG 3 cut(s) 18, 85, 391
Bst2UI CCWGG 4 cut(s) 425, 437, 528, 726
Bst4CI ACNGT 3 cut(s) 70, 319, 789
BstACI GRCGYC 1 cut(s) 363
BstBI TTCGAA 2 cut(s) 626, 740
BstC8I GCNNGC 3 cut(s) 169, 459, 672
BstDEI CTNAG 3 cut(s) 308, 651, 767
BstDSI CCRYGG 1 cut(s) 18
BstF5I GGATG 5 cut(s) 265, 446, 514, 667, 827
BstKTI GATC 2 cut(s) 76, 162
BstMAI GTCTC 1 cut(s) 589
BstMBI GATC 2 cut(s) 73, 159
BstMWI GCNNNNNNNGC 1 cut(s) 119
BstNI CCWGG 4 cut(s) 425, 437, 528, 726
BstSCI CCNGG 5 cut(s) 423, 435, 475, 526, 724
BstSFI CTRYAG 2 cut(s) 175, 470
BstV1I GCAGC 1 cut(s) 484
BstX2I RGATCY 1 cut(s) 159
BstYI RGATCY 1 cut(s) 159
Bsu15I ATCGAT 1 cut(s) 117
Bsu36I CCTNAGG 1 cut(s) 651
BsuI GTATCC 1 cut(s) 482
BsuRI GGCC 6 cut(s) 39, 84, 270, 417, 428, 681
BsuTUI ATCGAT 1 cut(s) 117
BtgI CCRYGG 1 cut(s) 18
BtsCI GGATG 5 cut(s) 265, 446, 514, 667, 827
BtsIMutI CAGTG 2 cut(s) 315, 459
Cac8I GCNNGC 3 cut(s) 169, 459, 672
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 3 cut(s) 269, 688, 722
ClaI ATCGAT 1 cut(s) 117
CseI GACGC 1 cut(s) 371
CsiI ACCWGGT 1 cut(s) 526
Csp6I GTAC 2 cut(s) 211, 564
CviAII CATG 5 cut(s) 19, 419, 560, 591, 683
CviQI GTAC 2 cut(s) 211, 564
DdeI CTNAG 3 cut(s) 308, 651, 767
DpnI GATC 2 cut(s) 75, 161
DpnII GATC 2 cut(s) 73, 159
DraIII CACNNNGTG 1 cut(s) 347
DrdI GACNNNNNNGTC 1 cut(s) 644
DseDI GACNNNNNNGTC 1 cut(s) 644
EaeI YGGCCR 4 cut(s) 37, 82, 415, 679
EciI GGCGGA 1 cut(s) 154
Eco130I CCWWGG 3 cut(s) 18, 85, 391
Eco47I GGWCC 2 cut(s) 688, 722
Eco57I CTGAAG 1 cut(s) 321
Eco81I CCTNAGG 1 cut(s) 651
Eco88I CYCGRG 1 cut(s) 604
EcoRII CCWGG 4 cut(s) 423, 435, 526, 724
EcoT14I CCWWGG 3 cut(s) 18, 85, 391
ErhI CCWWGG 3 cut(s) 18, 85, 391
FaeI CATG 5 cut(s) 22, 422, 563, 594, 686
FalI AAGNNNNNCTT 2 cut(s) 274, 306
FaqI GGGAC 1 cut(s) 632
FatI CATG 5 cut(s) 18, 418, 559, 590, 682
FauI CCCGC 1 cut(s) 454
Fnu4HI GCNGC 1 cut(s) 473
FokI GGATG 5 cut(s) 272, 453, 501, 674, 834
Fsp4HI GCNGC 1 cut(s) 473
FspBI CTAG 1 cut(s) 795
GluI GCNGC 1 cut(s) 473
HaeIII GGCC 6 cut(s) 39, 84, 270, 417, 428, 681
HapII CCGG 3 cut(s) 81, 168, 477
HgaI GACGC 1 cut(s) 371
Hin1I GRCGYC 1 cut(s) 363
Hin1II CATG 5 cut(s) 22, 422, 563, 594, 686
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 1 cut(s) 764
HinfI GANTC 2 cut(s) 623, 812
HpaII CCGG 3 cut(s) 81, 168, 477
HphI GGTGA 4 cut(s) 58, 88, 199, 239
Hpy166II GTNNAC 2 cut(s) 277, 792
Hpy188I TCNGA 4 cut(s) 222, 301, 637, 817
Hpy188III TCNNGA 3 cut(s) 157, 544, 781
Hpy8I GTNNAC 2 cut(s) 277, 792
HpyAV CCTTC 4 cut(s) 138, 178, 352, 579
HpyCH4III ACNGT 3 cut(s) 70, 319, 789
HpyCH4V TGCA 5 cut(s) 153, 383, 472, 552, 559
HpyF10VI GCNNNNNNNGC 1 cut(s) 119
HpyF3I CTNAG 3 cut(s) 308, 651, 767
Hsp92I GRCGYC 1 cut(s) 363
Hsp92II CATG 5 cut(s) 22, 422, 563, 594, 686
KroI GCCGGC 1 cut(s) 167
KroNI GCCGGC 1 cut(s) 169
Kzo9I GATC 2 cut(s) 73, 159
LmnI GCTCC 2 cut(s) 449, 728
Lsp1109I GCAGC 1 cut(s) 484
LweI GCATC 3 cut(s) 109, 162, 431
MabI ACCWGGT 1 cut(s) 526
MaeI CTAG 1 cut(s) 795
MalI GATC 2 cut(s) 75, 161
MboI GATC 2 cut(s) 73, 159
MboII GAAGA 2 cut(s) 3, 6
MfeI CAATTG 1 cut(s) 692
MflI RGATCY 1 cut(s) 159
MlsI TGGCCA 2 cut(s) 417, 681
MluCI AATT 5 cut(s) 47, 249, 547, 692, 802
MluNI TGGCCA 2 cut(s) 417, 681
MnlI CCTC 3 cut(s) 600, 660, 679
Mox20I TGGCCA 2 cut(s) 417, 681
MroNI GCCGGC 1 cut(s) 167
MscI TGGCCA 2 cut(s) 417, 681
MseI TTAA 1 cut(s) 572
MslI CAYNNNNRTG 2 cut(s) 452, 519
Msp20I TGGCCA 2 cut(s) 417, 681
MspI CCGG 3 cut(s) 81, 168, 477
MspR9I CCNGG 5 cut(s) 425, 437, 477, 528, 726
MunI CAATTG 1 cut(s) 692
MvaI CCWGG 4 cut(s) 425, 437, 528, 726
MwoI GCNNNNNNNGC 1 cut(s) 119
NaeI GCCGGC 1 cut(s) 169
NciI CCSGG 1 cut(s) 477
NcoI CCATGG 1 cut(s) 18
NdeII GATC 2 cut(s) 73, 159
NgoMIV GCCGGC 1 cut(s) 167
NlaIII CATG 5 cut(s) 22, 422, 563, 594, 686
NlaIV GGNNCC 2 cut(s) 172, 730
NmeAIII GCCGAG 1 cut(s) 730
NspV TTCGAA 2 cut(s) 626, 740
PaeR7I CTCGAG 1 cut(s) 604
PdiI GCCGGC 1 cut(s) 169
PfeI GAWTC 2 cut(s) 623, 812
PfoI TCCNGGA 1 cut(s) 724
PkrI GCNGC 1 cut(s) 474
Psp6I CCWGG 4 cut(s) 423, 435, 526, 724
PspGI CCWGG 4 cut(s) 423, 435, 526, 724
PspN4I GGNNCC 2 cut(s) 172, 730
PspPI GGNCC 3 cut(s) 269, 688, 722
PspXI VCTCGAGB 1 cut(s) 604
PstI CTGCAG 1 cut(s) 474
PsuI RGATCY 1 cut(s) 159
RsaI GTAC 2 cut(s) 212, 565
RsaNI GTAC 2 cut(s) 211, 564
RseI CAYNNNNRTG 2 cut(s) 452, 519
SaqAI TTAA 1 cut(s) 572
SatI GCNGC 1 cut(s) 473
Sau3AI GATC 2 cut(s) 73, 159
Sau96I GGNCC 3 cut(s) 269, 688, 722
ScrFI CCNGG 5 cut(s) 425, 437, 477, 528, 726
SetI ASST 8 cut(s) 176, 189, 229, 397, 529, 578, 768, 800
SexAI ACCWGGT 1 cut(s) 526
SfaNI GCATC 3 cut(s) 109, 162, 431
SfcI CTRYAG 2 cut(s) 175, 470
Sfr274I CTCGAG 1 cut(s) 604
SfuI TTCGAA 2 cut(s) 626, 740
SinI GGWCC 2 cut(s) 688, 722
SlaI CTCGAG 1 cut(s) 604
SmiMI CAYNNNNRTG 2 cut(s) 452, 519
SmlI CTYRAG 1 cut(s) 604
SmoI CTYRAG 1 cut(s) 604
Sse9I AATT 5 cut(s) 47, 249, 547, 692, 802
SsiI CCGC 3 cut(s) 165, 352, 461
SspMI CTAG 1 cut(s) 795
StyD4I CCNGG 5 cut(s) 423, 435, 475, 526, 724
StyI CCWWGG 3 cut(s) 18, 85, 391
TaaI ACNGT 3 cut(s) 70, 319, 789
TaqI TCGA 4 cut(s) 117, 605, 626, 740
TasI AATT 5 cut(s) 47, 249, 547, 692, 802
TfiI GAWTC 2 cut(s) 623, 812
Tru1I TTAA 1 cut(s) 572
Tru9I TTAA 1 cut(s) 572
TscAI CASTG 2 cut(s) 322, 459
TseI GCWGC 1 cut(s) 472
TspDTI ATGAA 2 cut(s) 537, 796
TspRI CASTG 2 cut(s) 322, 459
VpaK11BI GGWCC 2 cut(s) 688, 722
XapI RAATTY 1 cut(s) 802
XhoI CTCGAG 1 cut(s) 604
XspI CTAG 1 cut(s) 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.