AT3G25160

ER lumen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0015838
Physical Location & Seq
Reverse (-)
9160168 .. 9162220
2053 bp
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UTR
Exon/CDS
Intron
AT3G25160.1

Sequence Viewer

Length: 819 bp
ATGGGGAAGAGAAGAGGTGTCTCCGCCGTGAACGTTGTTTTCGGGTGGCTGCGACAACAATCGAACAAGGTTAAAATAGCTTTAGGAATTATCATCTCTTTGATCCTCGTCGTTCTTCTGAAATTCACCGTTAGGAATCACAATCACTTTTTCATCGCCTCCGAGCTCATCCACGCCGCCGGAATCTTGATCCTCATCTACAAACTCACTCGCCAAAAGACTTGCTCCGGTCTTTCTTTAAAATCTCAAGAAGTGACAGCAATATTTCTAGCGGTGAGATTGATATGCAGCATAAACATGGAAGGTGATATCCATACAGTTCTCGATTTCGCCACCTTGGTTTCGACTCTATGGGTCATTTATATGATTCGATACAAGCTGAAAGCATCTTATATAAAGTCTCTCGACACTTGCTACAATTACTATGTGCTTGTACCATCTGCTATCCTTGCGTTGATTATTAACCCGAGTACGAGTTATTCTTACTTCCACCGTATATTGTGGGCGTTTTGCGTCTACACAGAATCTGTCTCTGTTCTACCTCAACTTCGATTAATGCAAAATGCTCAGATCATAGAGCCTTTCACAGCTCATTATGTGTTTGCACTCGGAATAGCTAGATTCCTGGCATGCGCTCATTGGATTATCCAGGTCGTTGAGACTCGTGGACATTACCTATGGCTACTCGGGGCTGGTTACTTCTGGTTTCCGGTGGCTCTACTAGCCGAGATCGTTCAAACTTTCATCCTCGCCGACTTTTGCTACTACTATGTCAAAAGTGTTATGGAGGGTCAGCTTGTACTGAAGATGCCGGTTTAA

Protein Analysis

272

Amino Acids

31.03

Weight (kDa)

9.41

Isoelectric Point (pI)

42.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ER_lumen_recept PF00810 74 - 217 5.6e-41 ER lumen protein retaining receptor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015838)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19970 AT1G19970 AT3G25160
fragaria_vesca FvH4_2g18430
malus_domestica MD05G1170200.v1.1
prunus_persica Prupe.8G197400_v2.0.a1
pyrus_communis pycom05g15740
rosa_chinensis RchiOBHm_Chr6g0283911
rosa_laevigata RLG00000012787
rosa_rugosa Rorug06G0158400
rosa_samantha Rh6AG271400 Rh6BG272600 Rh6CG273800 Rh6DG266700
rosa_wichuraiana Rw6G023410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 516
AciI CCGC 3 cut(s) 24, 177, 272
AclI AACGTT 1 cut(s) 33
AclWI GGATC 2 cut(s) 97, 184
AcsI RAATTY 1 cut(s) 122
AfaI GTAC 3 cut(s) 435, 472, 801
AgsI TTSAA 1 cut(s) 737
AjnI CCWGG 2 cut(s) 624, 648
AluBI AGCT 6 cut(s) 80, 166, 379, 590, 617, 796
AluI AGCT 6 cut(s) 80, 166, 379, 590, 617, 796
Alw21I GWGCWC 1 cut(s) 168
Alw26I GTCTC 4 cut(s) 25, 405, 535, 653
AlwI GGATC 2 cut(s) 97, 184
AlwNI CAGNNNCTG 1 cut(s) 527
Ama87I CYCGRG 2 cut(s) 466, 686
ApeKI GCWGC 2 cut(s) 49, 288
ApoI RAATTY 1 cut(s) 122
AseI ATTAAT 1 cut(s) 554
AspLEI GCGC 1 cut(s) 635
AsuHPI GGTGA 3 cut(s) 118, 286, 317
AvaI CYCGRG 2 cut(s) 466, 686
BanII GRGCYC 1 cut(s) 168
BauI CACGAG 1 cut(s) 663
Bbv12I GWGCWC 1 cut(s) 168
BbvI GCAGC 2 cut(s) 36, 300
BccI CCATC 1 cut(s) 445
BceAI ACGGC 1 cut(s) 11
BciT130I CCWGG 2 cut(s) 626, 650
BcoDI GTCTC 4 cut(s) 25, 405, 535, 653
BfaI CTAG 3 cut(s) 269, 618, 722
BisI GCNGC 3 cut(s) 50, 177, 289
BlsI GCNGC 3 cut(s) 51, 178, 290
Bme1390I CCNGG 2 cut(s) 626, 650
BmeT110I CYCGRG 2 cut(s) 466, 686
BmrFI CCNGG 2 cut(s) 626, 650
BmsI GCATC 2 cut(s) 395, 798
BpuEI CTTGAG 1 cut(s) 231
BsaBI GATNNNNATC 1 cut(s) 194
BsaJI CCNNGG 1 cut(s) 336
BsaWI WCCGGW 2 cut(s) 227, 709
Bse118I RCCGGY 1 cut(s) 811
Bse8I GATNNNNATC 1 cut(s) 194
BseBI CCWGG 2 cut(s) 626, 650
BseDI CCNNGG 1 cut(s) 336
BseGI GGATG 2 cut(s) 168, 744
BseJI GATNNNNATC 1 cut(s) 194
BseMII CTCAG 1 cut(s) 581
BseXI GCAGC 2 cut(s) 36, 300
BsiHKAI GWGCWC 1 cut(s) 168
BsiHKCI CYCGRG 2 cut(s) 466, 686
BsiSI CCGG 4 cut(s) 180, 228, 710, 812
BsmAI GTCTC 4 cut(s) 25, 405, 535, 653
BsoBI CYCGRG 2 cut(s) 466, 686
Bsp1286I GDGCHC 1 cut(s) 168
Bsp143I GATC 4 cut(s) 102, 189, 570, 729
BspACI CCGC 3 cut(s) 24, 177, 272
BspCNI CTCAG 1 cut(s) 580
BspPI GGATC 2 cut(s) 97, 184
BsrFI RCCGGY 1 cut(s) 811
BssAI RCCGGY 1 cut(s) 811
BssECI CCNNGG 1 cut(s) 336
BssMI GATC 4 cut(s) 102, 189, 570, 729
BssSI CACGAG 1 cut(s) 663
BssT1I CCWWGG 1 cut(s) 336
Bst2BI CACGAG 1 cut(s) 663
Bst2UI CCWGG 2 cut(s) 626, 650
Bst4CI ACNGT 3 cut(s) 130, 319, 494
Bst6I CTCTTC 2 cut(s) 2, 7
BstC8I GCNNGC 1 cut(s) 631
BstDEI CTNAG 1 cut(s) 567
BstF5I GGATG 2 cut(s) 168, 744
BstHHI GCGC 1 cut(s) 635
BstKTI GATC 4 cut(s) 105, 192, 573, 732
BstMAI GTCTC 4 cut(s) 25, 405, 535, 653
BstMBI GATC 4 cut(s) 102, 189, 570, 729
BstMWI GCNNNNNNNGC 2 cut(s) 449, 722
BstNI CCWGG 2 cut(s) 626, 650
BstNSI RCATGY 1 cut(s) 633
BstSCI CCNGG 2 cut(s) 624, 648
BstV1I GCAGC 2 cut(s) 36, 300
BtgZI GCGATG 1 cut(s) 139
BtsCI GGATG 2 cut(s) 168, 744
Cac8I GCNNGC 1 cut(s) 631
CaiI CAGNNNCTG 1 cut(s) 527
CfoI GCGC 1 cut(s) 635
Cfr10I RCCGGY 1 cut(s) 811
CseI GACGC 1 cut(s) 502
Csp6I GTAC 3 cut(s) 434, 471, 800
CviAII CATG 2 cut(s) 298, 630
CviQI GTAC 3 cut(s) 434, 471, 800
DdeI CTNAG 1 cut(s) 567
DpnI GATC 4 cut(s) 104, 191, 572, 731
DpnII GATC 4 cut(s) 102, 189, 570, 729
DraI TTTAAA 1 cut(s) 240
Eam1104I CTCTTC 2 cut(s) 2, 7
EarI CTCTTC 2 cut(s) 2, 7
EciI GGCGGA 1 cut(s) 13
Ecl136II GAGCTC 1 cut(s) 166
Eco130I CCWWGG 1 cut(s) 336
Eco24I GRGCYC 1 cut(s) 168
Eco32I GATATC 1 cut(s) 310
Eco53kI GAGCTC 1 cut(s) 166
Eco88I CYCGRG 2 cut(s) 466, 686
EcoICRI GAGCTC 1 cut(s) 166
EcoRII CCWGG 2 cut(s) 624, 648
EcoRV GATATC 1 cut(s) 310
EcoT14I CCWWGG 1 cut(s) 336
EcoT38I GRGCYC 1 cut(s) 168
ErhI CCWWGG 1 cut(s) 336
FaeI CATG 2 cut(s) 301, 633
FatI CATG 2 cut(s) 297, 629
FblI GTMKAC 1 cut(s) 516
Fnu4HI GCNGC 3 cut(s) 50, 177, 289
FokI GGATG 2 cut(s) 155, 731
FriOI GRGCYC 1 cut(s) 168
Fsp4HI GCNGC 3 cut(s) 50, 177, 289
FspBI CTAG 3 cut(s) 269, 618, 722
GlaI GCGC 1 cut(s) 634
GluI GCNGC 3 cut(s) 50, 177, 289
HapII CCGG 4 cut(s) 180, 228, 710, 812
HgaI GACGC 1 cut(s) 502
HhaI GCGC 1 cut(s) 635
Hin1II CATG 2 cut(s) 301, 633
Hin6I GCGC 1 cut(s) 633
HinP1I GCGC 1 cut(s) 633
HinfI GANTC 7 cut(s) 136, 183, 346, 367, 524, 621, 661
HpaII CCGG 4 cut(s) 180, 228, 710, 812
HphI GGTGA 3 cut(s) 118, 286, 317
Hpy166II GTNNAC 3 cut(s) 31, 517, 668
Hpy188I TCNGA 4 cut(s) 120, 163, 570, 611
Hpy188III TCNNGA 4 cut(s) 187, 248, 323, 404
Hpy8I GTNNAC 3 cut(s) 31, 517, 668
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 1 cut(s) 296
HpyCH4III ACNGT 3 cut(s) 130, 319, 494
HpyCH4IV ACGT 1 cut(s) 33
HpyCH4V TGCA 3 cut(s) 288, 559, 605
HpyF10VI GCNNNNNNNGC 2 cut(s) 449, 722
HpyF3I CTNAG 1 cut(s) 567
HpySE526I ACGT 1 cut(s) 33
Hsp92II CATG 2 cut(s) 301, 633
HspAI GCGC 1 cut(s) 633
Kzo9I GATC 4 cut(s) 102, 189, 570, 729
LmnI GCTCC 1 cut(s) 230
LpnPI CCDG 9 cut(s) 193, 241, 611, 635, 638, 662, 678, 688, 723
Lsp1109I GCAGC 2 cut(s) 36, 300
LweI GCATC 2 cut(s) 395, 798
MaeI CTAG 3 cut(s) 269, 618, 722
MaeII ACGT 1 cut(s) 33
MaeIII GTNAC 2 cut(s) 253, 695
MalI GATC 4 cut(s) 104, 191, 572, 731
MboI GATC 4 cut(s) 102, 189, 570, 729
MboII GAAGA 4 cut(s) 19, 24, 107, 817
MhlI GDGCHC 1 cut(s) 168
MluCI AATT 3 cut(s) 87, 122, 418
MlyI GAGTC 2 cut(s) 340, 655
MnlI CCTC 7 cut(s) 8, 116, 169, 203, 552, 758, 781
MseI TTAA 5 cut(s) 72, 239, 462, 554, 817
MslI CAYNNNNRTG 2 cut(s) 296, 362
MspI CCGG 4 cut(s) 180, 228, 710, 812
MspR9I CCNGG 2 cut(s) 626, 650
MvaI CCWGG 2 cut(s) 626, 650
MwoI GCNNNNNNNGC 2 cut(s) 449, 722
NdeII GATC 4 cut(s) 102, 189, 570, 729
NlaIII CATG 2 cut(s) 301, 633
NmeAIII GCCGAG 1 cut(s) 751
NmuCI GTSAC 1 cut(s) 253
NspI RCATGY 1 cut(s) 633
PaeI GCATGC 1 cut(s) 633
PfeI GAWTC 5 cut(s) 136, 183, 367, 524, 621
PkrI GCNGC 3 cut(s) 51, 178, 290
PleI GAGTC 2 cut(s) 340, 655
PpsI GAGTC 2 cut(s) 340, 655
PshBI ATTAAT 1 cut(s) 554
Psp124BI GAGCTC 1 cut(s) 168
Psp1406I AACGTT 1 cut(s) 33
Psp6I CCWGG 2 cut(s) 624, 648
PspGI CCWGG 2 cut(s) 624, 648
PstNI CAGNNNCTG 1 cut(s) 527
RsaI GTAC 3 cut(s) 435, 472, 801
RsaNI GTAC 3 cut(s) 434, 471, 800
RseI CAYNNNNRTG 2 cut(s) 296, 362
SacI GAGCTC 1 cut(s) 168
SaqAI TTAA 5 cut(s) 72, 239, 462, 554, 817
SatI GCNGC 3 cut(s) 50, 177, 289
Sau3AI GATC 4 cut(s) 102, 189, 570, 729
SchI GAGTC 2 cut(s) 340, 655
ScrFI CCNGG 2 cut(s) 626, 650
SduI GDGCHC 1 cut(s) 168
SfaNI GCATC 2 cut(s) 395, 798
SmiMI CAYNNNNRTG 2 cut(s) 296, 362
SmlI CTYRAG 1 cut(s) 246
SmoI CTYRAG 1 cut(s) 246
SphI GCATGC 1 cut(s) 633
Sse9I AATT 3 cut(s) 87, 122, 418
SsiI CCGC 3 cut(s) 24, 177, 272
SspI AATATT 1 cut(s) 264
SspMI CTAG 3 cut(s) 269, 618, 722
SstI GAGCTC 1 cut(s) 168
StyD4I CCNGG 2 cut(s) 624, 648
StyI CCWWGG 1 cut(s) 336
TaaI ACNGT 3 cut(s) 130, 319, 494
TaiI ACGT 1 cut(s) 36
TaqI TCGA 6 cut(s) 62, 324, 344, 370, 405, 550
TasI AATT 3 cut(s) 87, 122, 418
TatI WGTACW 1 cut(s) 799
TauI GCSGC 1 cut(s) 179
TfiI GAWTC 5 cut(s) 136, 183, 367, 524, 621
Tru1I TTAA 5 cut(s) 72, 239, 462, 554, 817
Tru9I TTAA 5 cut(s) 72, 239, 462, 554, 817
TseFI GTSAC 1 cut(s) 253
TseI GCWGC 2 cut(s) 49, 288
Tsp45I GTSAC 1 cut(s) 253
TspDTI ATGAA 2 cut(s) 142, 733
VspI ATTAAT 1 cut(s) 554
XapI RAATTY 1 cut(s) 122
XceI RCATGY 1 cut(s) 633
XmiI GTMKAC 1 cut(s) 516
XspI CTAG 3 cut(s) 269, 618, 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.