Rh6AG271400

ER lumen protein retaining

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0015838
Physical Location & Seq
Forward (+)
46566170 .. 46570014
3845 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG271400.1

Sequence Viewer

Length: 780 bp
ATGGGGAGGAGGAGGAACGCTTCAGTGAATGTTCTGTTTCAATGGGTGAGGAGGCAGTCAATGAAAGTCAAGATCTTTCTTGGAGCCTTTCTTGCGCTGTGTGGTTTGGTGGCACTGAAGTTTTGGGTCAAGAATCATGACCATTTTTTCATTGCCTCTGAAGCCATTCATTTTCTGGGGATCATCGTCCTCATATACAAGCTCACAACCCAAAAGACCTGCTCTGGTCTTTCCCTAAAGACTCAAGAACTCACAGCTGCCTTTGTAGCAGTAAGATTGGTTTGCAGTACCATCATGGAGGCTGATGTGCACACTGTGCTAGATTTTGCTACTCTCATTTCAACAGCTTGGCTCATTTTTATGATACGGTTCAAACTGAAGTCAACCTACATAAAGGAACTCGACAACTTTCGCTTATATTTTGTGGTGGTGCCTGCTGCTATCCTTGCCCTGGTTTTCCACCCATTCATGCTGAGTGGTCGTATTACTCGAGTTCTTTGGGCATTTGGTGGGTACATAGAAGCTGTTTCAGTACTACCACAGCTTCGTTTGATGCAGAATGCAAAGATGATTGAACCATTTACCTCCCATTATGTATTTGCATTGGGTATTTCAAGATTCTTTGCTTGTGCTCATTGGATCATTATGATCTACGAAACTAAAGGCAAATATCTATTCTTGGTTGGGAGTGGTTATTCGTGGATTTTGGCTGCCTTGATCTGTGAAATTGTTCAAACATTCATCTTAGCAGACTTTTGTTACTATTACATAAAGAGGTAA

Protein Analysis

259

Amino Acids

29.78

Weight (kDa)

9.67

Isoelectric Point (pI)

30.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ER_lumen_recept PF00810 73 - 215 1.6e-41 ER lumen protein retaining receptor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015838)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19970 AT1G19970 AT3G25160
fragaria_vesca FvH4_2g18430
malus_domestica MD05G1170200.v1.1
prunus_persica Prupe.8G197400_v2.0.a1
pyrus_communis pycom05g15740
rosa_chinensis RchiOBHm_Chr6g0283911
rosa_laevigata RLG00000012787
rosa_rugosa Rorug06G0158400
rosa_samantha Rh6AG271400 Rh6BG272600 Rh6CG273800 Rh6DG266700
rosa_wichuraiana Rw6G023410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 227
AccB1I GGYRCC 1 cut(s) 430
AclWI GGATC 2 cut(s) 188, 647
AcuI CTGAAG 4 cut(s) 6, 137, 180, 398
AdeI CACNNNGTG 1 cut(s) 316
AfaI GTAC 3 cut(s) 289, 515, 534
AfiI CCNNNNNNNGG 1 cut(s) 451
AgsI TTSAA 6 cut(s) 41, 342, 373, 575, 615, 734
AjnI CCWGG 1 cut(s) 450
AluBI AGCT 5 cut(s) 202, 257, 347, 524, 544
AluI AGCT 5 cut(s) 202, 257, 347, 524, 544
Alw21I GWGCWC 2 cut(s) 312, 634
Alw44I GTGCAC 1 cut(s) 308
AlwI GGATC 2 cut(s) 188, 647
Ama87I CYCGRG 1 cut(s) 489
ApaLI GTGCAC 1 cut(s) 308
ApeKI GCWGC 3 cut(s) 257, 437, 710
Asp700I GAANNNNTTC 2 cut(s) 165, 729
AspLEI GCGC 1 cut(s) 97
AsuHPI GGTGA 1 cut(s) 58
AvaI CYCGRG 1 cut(s) 489
BaeGI GKGCMC 1 cut(s) 312
BanI GGYRCC 1 cut(s) 430
BarI GAAGNNNNNNTAC 2 cut(s) 371, 403
Bbv12I GWGCWC 2 cut(s) 312, 634
BbvI GCAGC 3 cut(s) 244, 424, 697
BccI CCATC 1 cut(s) 299
BciT130I CCWGG 1 cut(s) 452
BfaI CTAG 1 cut(s) 320
BfuAI ACCTGC 1 cut(s) 227
BglII AGATCT 1 cut(s) 72
BisI GCNGC 3 cut(s) 258, 438, 711
BlsI GCNGC 3 cut(s) 259, 439, 712
BmcAI AGTACT 1 cut(s) 534
Bme1390I CCNGG 1 cut(s) 452
BmeT110I CYCGRG 1 cut(s) 489
BmiI GGNNCC 2 cut(s) 85, 432
BmrFI CCNGG 1 cut(s) 452
BmsI GCATC 1 cut(s) 543
BpuEI CTTGAG 1 cut(s) 228
BsaJI CCNNGG 1 cut(s) 450
Bsc4I CCNNNNNNNGG 1 cut(s) 451
Bse3DI GCAATG 1 cut(s) 150
BseBI CCWGG 1 cut(s) 452
BseDI CCNNGG 1 cut(s) 450
BseLI CCNNNNNNNGG 1 cut(s) 451
BseMI GCAATG 1 cut(s) 150
BseMII CTCAG 1 cut(s) 464
BseRI GAGGAG 3 cut(s) 22, 25, 64
BseSI GKGCMC 1 cut(s) 312
BseXI GCAGC 3 cut(s) 244, 424, 697
BshNI GGYRCC 1 cut(s) 430
BsiHKAI GWGCWC 2 cut(s) 312, 634
BsiHKCI CYCGRG 1 cut(s) 489
BslI CCNNNNNNNGG 1 cut(s) 451
BsmI GAATGC 1 cut(s) 565
BsoBI CYCGRG 1 cut(s) 489
Bsp1286I GDGCHC 2 cut(s) 312, 634
Bsp143I GATC 5 cut(s) 72, 180, 639, 648, 717
BspCNI CTCAG 1 cut(s) 465
BspHI TCATGA 1 cut(s) 136
BspLI GGNNCC 2 cut(s) 85, 432
BspMI ACCTGC 1 cut(s) 227
BspPI GGATC 2 cut(s) 188, 647
BspT107I GGYRCC 1 cut(s) 430
BsrDI GCAATG 1 cut(s) 150
BssECI CCNNGG 1 cut(s) 450
BssMI GATC 5 cut(s) 72, 180, 639, 648, 717
Bst2UI CCWGG 1 cut(s) 452
Bst4CI ACNGT 2 cut(s) 316, 369
BstAPI GCANNNNNTGC 1 cut(s) 316
BstC8I GCNNGC 1 cut(s) 435
BstDEI CTNAG 2 cut(s) 473, 745
BstHHI GCGC 1 cut(s) 97
BstKTI GATC 5 cut(s) 75, 183, 642, 651, 720
BstMBI GATC 5 cut(s) 72, 180, 639, 648, 717
BstMWI GCNNNNNNNGC 5 cut(s) 92, 161, 266, 316, 446
BstNI CCWGG 1 cut(s) 452
BstSCI CCNGG 1 cut(s) 450
BstSLI GKGCMC 1 cut(s) 312
BstV1I GCAGC 3 cut(s) 244, 424, 697
BstX2I RGATCY 1 cut(s) 72
BstYI RGATCY 1 cut(s) 72
BtsIMutI CAGTG 3 cut(s) 30, 113, 312
BveI ACCTGC 1 cut(s) 227
Cac8I GCNNGC 1 cut(s) 435
CciI TCATGA 1 cut(s) 136
CfoI GCGC 1 cut(s) 97
Csp6I GTAC 3 cut(s) 288, 514, 533
CviAII CATG 3 cut(s) 137, 295, 469
CviQI GTAC 3 cut(s) 288, 514, 533
DdeI CTNAG 2 cut(s) 473, 745
DpnI GATC 5 cut(s) 74, 182, 641, 650, 719
DpnII GATC 5 cut(s) 72, 180, 639, 648, 717
DraIII CACNNNGTG 1 cut(s) 316
Eco57I CTGAAG 4 cut(s) 6, 137, 180, 398
Eco88I CYCGRG 1 cut(s) 489
EcoRII CCWGG 1 cut(s) 450
FaeI CATG 3 cut(s) 140, 298, 472
FatI CATG 3 cut(s) 136, 294, 468
Fnu4HI GCNGC 3 cut(s) 258, 438, 711
Fsp4HI GCNGC 3 cut(s) 258, 438, 711
FspBI CTAG 1 cut(s) 320
GlaI GCGC 1 cut(s) 96
GluI GCNGC 3 cut(s) 258, 438, 711
HhaI GCGC 1 cut(s) 97
Hin1II CATG 3 cut(s) 140, 298, 472
Hin6I GCGC 1 cut(s) 95
HinP1I GCGC 1 cut(s) 95
HincII GTYRAC 1 cut(s) 384
HindII GTYRAC 1 cut(s) 384
HinfI GANTC 3 cut(s) 133, 241, 618
HphI GGTGA 1 cut(s) 58
Hpy166II GTNNAC 2 cut(s) 310, 384
Hpy188I TCNGA 1 cut(s) 160
Hpy188III TCNNGA 5 cut(s) 70, 130, 137, 245, 615
Hpy8I GTNNAC 2 cut(s) 310, 384
HpyCH4III ACNGT 2 cut(s) 316, 369
HpyCH4V TGCA 5 cut(s) 285, 310, 556, 563, 602
HpyF10VI GCNNNNNNNGC 5 cut(s) 92, 161, 266, 316, 446
HpyF3I CTNAG 2 cut(s) 473, 745
Hsp92II CATG 3 cut(s) 140, 298, 472
HspAI GCGC 1 cut(s) 95
Kzo9I GATC 5 cut(s) 72, 180, 639, 648, 717
LmnI GCTCC 1 cut(s) 83
LpnPI CCDG 6 cut(s) 161, 210, 232, 437, 447, 464
Lsp1109I GCAGC 3 cut(s) 244, 424, 697
LweI GCATC 1 cut(s) 543
MaeI CTAG 1 cut(s) 320
MaeIII GTNAC 1 cut(s) 758
MalI GATC 5 cut(s) 74, 182, 641, 650, 719
MboI GATC 5 cut(s) 72, 180, 639, 648, 717
MflI RGATCY 1 cut(s) 72
MhlI GDGCHC 2 cut(s) 312, 634
MluCI AATT 1 cut(s) 726
MlyI GAGTC 1 cut(s) 235
MnlI CCTC 9 cut(s) 3, 6, 42, 45, 166, 200, 292, 595, 768
MroXI GAANNNNTTC 2 cut(s) 165, 729
MslI CAYNNNNRTG 1 cut(s) 359
MspA1I CMGCKG 1 cut(s) 257
MspR9I CCNGG 1 cut(s) 452
Mva1269I GAATGC 1 cut(s) 565
MvaI CCWGG 1 cut(s) 452
MwoI GCNNNNNNNGC 5 cut(s) 92, 161, 266, 316, 446
NdeII GATC 5 cut(s) 72, 180, 639, 648, 717
NlaIII CATG 3 cut(s) 140, 298, 472
NlaIV GGNNCC 2 cut(s) 85, 432
PaeR7I CTCGAG 1 cut(s) 489
PagI TCATGA 1 cut(s) 136
PcsI WCGNNNNNNNCGW 1 cut(s) 487
PctI GAATGC 1 cut(s) 565
PdmI GAANNNNTTC 2 cut(s) 165, 729
PfeI GAWTC 2 cut(s) 133, 618
PkrI GCNGC 3 cut(s) 259, 439, 712
PleI GAGTC 1 cut(s) 235
PpsI GAGTC 1 cut(s) 235
Psp6I CCWGG 1 cut(s) 450
PspGI CCWGG 1 cut(s) 450
PspN4I GGNNCC 2 cut(s) 85, 432
PspXI VCTCGAGB 1 cut(s) 489
PsuI RGATCY 1 cut(s) 72
PvuII CAGCTG 1 cut(s) 257
RsaI GTAC 3 cut(s) 289, 515, 534
RsaNI GTAC 3 cut(s) 288, 514, 533
RseI CAYNNNNRTG 1 cut(s) 359
SatI GCNGC 3 cut(s) 258, 438, 711
Sau3AI GATC 5 cut(s) 72, 180, 639, 648, 717
ScaI AGTACT 1 cut(s) 534
SchI GAGTC 1 cut(s) 235
ScrFI CCNGG 1 cut(s) 452
SduI GDGCHC 2 cut(s) 312, 634
SetI ASST 9 cut(s) 204, 221, 259, 349, 389, 526, 546, 587, 779
SfaNI GCATC 1 cut(s) 543
Sfr274I CTCGAG 1 cut(s) 489
SlaI CTCGAG 1 cut(s) 489
SmiMI CAYNNNNRTG 1 cut(s) 359
SmlI CTYRAG 2 cut(s) 243, 489
SmoI CTYRAG 2 cut(s) 243, 489
Sse9I AATT 1 cut(s) 726
SspMI CTAG 1 cut(s) 320
StyD4I CCNGG 1 cut(s) 450
TaaI ACNGT 2 cut(s) 316, 369
TaqI TCGA 2 cut(s) 402, 490
TasI AATT 1 cut(s) 726
TatI WGTACW 1 cut(s) 532
TfiI GAWTC 2 cut(s) 133, 618
TscAI CASTG 3 cut(s) 30, 120, 319
TseI GCWGC 3 cut(s) 257, 437, 710
TspDTI ATGAA 5 cut(s) 77, 139, 158, 457, 730
TspRI CASTG 3 cut(s) 30, 120, 319
VneI GTGCAC 1 cut(s) 308
XcmI CCANNNNNNNNNTGG 1 cut(s) 172
XhoI CTCGAG 1 cut(s) 489
XmnI GAANNNNTTC 2 cut(s) 165, 729
XspI CTAG 1 cut(s) 320
ZrmI AGTACT 1 cut(s) 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.