FvH4_2g18430

ER lumen protein retaining

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015838
Physical Location & Seq
Forward (+)
15795166 .. 15798334
3169 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g18430.t1

Sequence Viewer

Length: 816 bp
ATGGGGAGGAAGAGGAACGCTTCAGTGAATGTTCTGTTTCAGTGGGTGAGGGGGCAGTCAATGAAAGTCAAGATCTTTCTTGGAGCCTTTCTTGCCTTGTGTAGTTTGGTGGCTTTGAAGCTTTGGGTCAAAAATCATAAATATTTTTTTGTTGCCTCTGAAGCCATTCATTTTCTGGGGATCATCGTTCTCATATACAAGCTCACAATTCAAAAGACCTGCTCTGGTCTTTCCCTAAAGACTCAAGAACTCACAGCTGCCTTTCTAGTAGTAAGATTGGTTTGTAGTACCATTATAGAGGCTGATATACACACTGTGCTAGATTCTGCTACTCTCATTTCAACAGCTTGGCTCATTTATATGATACGGTTCAAGCTGAAGTCAACATATATAAAGGAACTCGACAACTTTCGCTTATATTTTGTGGTAGTGCCTGCTGCTATCCTTTCTTTGTTCTTCCACCCATACACACTGCAAGGTCTCATTGTTTCAGTTCTTTGGGCGTTTGGTGGCTACATAGAAGCTGTTTCCGTACTGCCACAGCTTCGCTTGATGCAGAATGCAAAGATGATTGAGCCATTTACCTCCCATTATGTATTTGCATTGGGTATTTCAAGATTCTTTGCTTGTGCTCATTGGATCATTATGATCTATGAAACTAGTGGCAGATATCTATCCATGGTTGGGAGTGGTTATCTCTGGATTTTGGCTGCCTTCCTGTGTGAAATGGTTCAAACATTCATCTTAGCAGACTTTTGCTACTATTACATAAAGAGTTATGTACAAGGTCAGCTCGTAATGAGGATGCCTGTCTAG

Protein Analysis

272

Amino Acids

31.01

Weight (kDa)

9.46

Isoelectric Point (pI)

32.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ER_lumen_recept PF00810 73 - 215 1.3e-41 ER lumen protein retaining receptor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015838)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19970 AT1G19970 AT3G25160
fragaria_vesca FvH4_2g18430
malus_domestica MD05G1170200.v1.1
prunus_persica Prupe.8G197400_v2.0.a1
pyrus_communis pycom05g15740
rosa_chinensis RchiOBHm_Chr6g0283911
rosa_laevigata RLG00000012787
rosa_rugosa Rorug06G0158400
rosa_samantha Rh6AG271400 Rh6BG272600 Rh6CG273800 Rh6DG266700
rosa_wichuraiana Rw6G023410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 227
AclWI GGATC 2 cut(s) 188, 647
AcuI CTGAAG 3 cut(s) 6, 180, 398
AdeI CACNNNGTG 1 cut(s) 316
AfaI GTAC 3 cut(s) 289, 534, 783
AfiI CCNNNNNNNGG 1 cut(s) 684
AgsI TTSAA 6 cut(s) 118, 212, 342, 373, 615, 734
AhlI ACTAGT 1 cut(s) 659
AluBI AGCT 8 cut(s) 121, 202, 257, 347, 376, 524, 544, 793
AluI AGCT 8 cut(s) 121, 202, 257, 347, 376, 524, 544, 793
Alw21I GWGCWC 1 cut(s) 634
Alw26I GTCTC 1 cut(s) 485
AlwI GGATC 2 cut(s) 188, 647
ApeKI GCWGC 3 cut(s) 257, 437, 710
Asp700I GAANNNNTTC 2 cut(s) 165, 729
AsuHPI GGTGA 1 cut(s) 58
Bbv12I GWGCWC 1 cut(s) 634
BbvI GCAGC 3 cut(s) 244, 424, 697
BcoDI GTCTC 1 cut(s) 485
BcuI ACTAGT 1 cut(s) 659
BfaI CTAG 4 cut(s) 266, 320, 660, 814
BfuAI ACCTGC 1 cut(s) 227
BglII AGATCT 1 cut(s) 72
BisI GCNGC 3 cut(s) 258, 438, 711
BlsI GCNGC 3 cut(s) 259, 439, 712
BmiI GGNNCC 1 cut(s) 85
BmsI GCATC 2 cut(s) 543, 795
BpuEI CTTGAG 1 cut(s) 228
BsaBI GATNNNNATC 1 cut(s) 673
BsaI GGTCTC 1 cut(s) 485
BsaJI CCNNGG 1 cut(s) 678
Bsc4I CCNNNNNNNGG 1 cut(s) 684
Bse8I GATNNNNATC 1 cut(s) 673
BseDI CCNNGG 1 cut(s) 678
BseGI GGATG 1 cut(s) 810
BseJI GATNNNNATC 1 cut(s) 673
BseLI CCNNNNNNNGG 1 cut(s) 684
BseXI GCAGC 3 cut(s) 244, 424, 697
BsiHKAI GWGCWC 1 cut(s) 634
BslI CCNNNNNNNGG 1 cut(s) 684
BsmAI GTCTC 1 cut(s) 485
BsmI GAATGC 1 cut(s) 565
Bso31I GGTCTC 1 cut(s) 485
Bsp1286I GDGCHC 1 cut(s) 634
Bsp1407I TGTACA 1 cut(s) 781
Bsp143I GATC 4 cut(s) 72, 180, 639, 648
Bsp19I CCATGG 1 cut(s) 678
BspLI GGNNCC 1 cut(s) 85
BspMI ACCTGC 1 cut(s) 227
BspPI GGATC 2 cut(s) 188, 647
BspTNI GGTCTC 1 cut(s) 485
BsrGI TGTACA 1 cut(s) 781
BssECI CCNNGG 1 cut(s) 678
BssMI GATC 4 cut(s) 72, 180, 639, 648
BssT1I CCWWGG 1 cut(s) 678
Bst4CI ACNGT 2 cut(s) 316, 369
Bst6I CTCTTC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 781
BstC8I GCNNGC 1 cut(s) 435
BstDEI CTNAG 1 cut(s) 745
BstDSI CCRYGG 1 cut(s) 678
BstF5I GGATG 1 cut(s) 810
BstKTI GATC 4 cut(s) 75, 183, 642, 651
BstMAI GTCTC 1 cut(s) 485
BstMBI GATC 4 cut(s) 72, 180, 639, 648
BstMWI GCNNNNNNNGC 2 cut(s) 92, 161
BstV1I GCAGC 3 cut(s) 244, 424, 697
BstX2I RGATCY 1 cut(s) 72
BstYI RGATCY 1 cut(s) 72
BtgI CCRYGG 1 cut(s) 678
BtsCI GGATG 1 cut(s) 810
BtsI GCAGTG 1 cut(s) 470
BtsIMutI CAGTG 4 cut(s) 30, 47, 312, 470
BveI ACCTGC 1 cut(s) 227
Cac8I GCNNGC 1 cut(s) 435
Csp6I GTAC 3 cut(s) 288, 533, 782
CviAII CATG 1 cut(s) 679
CviQI GTAC 3 cut(s) 288, 533, 782
DdeI CTNAG 1 cut(s) 745
DpnI GATC 4 cut(s) 74, 182, 641, 650
DpnII GATC 4 cut(s) 72, 180, 639, 648
DraIII CACNNNGTG 1 cut(s) 316
Eam1104I CTCTTC 1 cut(s) 5
EarI CTCTTC 1 cut(s) 5
Eco130I CCWWGG 1 cut(s) 678
Eco31I GGTCTC 1 cut(s) 485
Eco32I GATATC 1 cut(s) 671
Eco57I CTGAAG 3 cut(s) 6, 180, 398
EcoRV GATATC 1 cut(s) 671
EcoT14I CCWWGG 1 cut(s) 678
ErhI CCWWGG 1 cut(s) 678
FaeI CATG 1 cut(s) 682
FatI CATG 1 cut(s) 678
Fnu4HI GCNGC 3 cut(s) 258, 438, 711
Fsp4HI GCNGC 3 cut(s) 258, 438, 711
FspBI CTAG 4 cut(s) 266, 320, 660, 814
GluI GCNGC 3 cut(s) 258, 438, 711
Hin1II CATG 1 cut(s) 682
HincII GTYRAC 1 cut(s) 384
HindII GTYRAC 1 cut(s) 384
HindIII AAGCTT 1 cut(s) 119
HinfI GANTC 3 cut(s) 241, 323, 618
HphI GGTGA 1 cut(s) 58
Hpy166II GTNNAC 1 cut(s) 384
Hpy188I TCNGA 1 cut(s) 160
Hpy188III TCNNGA 4 cut(s) 70, 245, 615, 700
Hpy8I GTNNAC 1 cut(s) 384
HpyAV CCTTC 1 cut(s) 724
HpyCH4III ACNGT 2 cut(s) 316, 369
HpyCH4V TGCA 4 cut(s) 475, 556, 563, 602
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 161
HpyF3I CTNAG 1 cut(s) 745
Hsp92II CATG 1 cut(s) 682
Kzo9I GATC 4 cut(s) 72, 180, 639, 648
LmnI GCTCC 1 cut(s) 83
LpnPI CCDG 6 cut(s) 161, 210, 232, 447, 685, 731
Lsp1109I GCAGC 3 cut(s) 244, 424, 697
LweI GCATC 2 cut(s) 543, 795
MaeI CTAG 4 cut(s) 266, 320, 660, 814
MalI GATC 4 cut(s) 74, 182, 641, 650
MboI GATC 4 cut(s) 72, 180, 639, 648
MboII GAAGA 2 cut(s) 22, 448
MflI RGATCY 1 cut(s) 72
MhlI GDGCHC 1 cut(s) 634
MluCI AATT 1 cut(s) 207
MlyI GAGTC 1 cut(s) 235
MnlI CCTC 6 cut(s) 6, 42, 166, 292, 595, 795
MroXI GAANNNNTTC 2 cut(s) 165, 729
MslI CAYNNNNRTG 1 cut(s) 359
MspA1I CMGCKG 1 cut(s) 257
Mva1269I GAATGC 1 cut(s) 565
MwoI GCNNNNNNNGC 2 cut(s) 92, 161
NcoI CCATGG 1 cut(s) 678
NdeII GATC 4 cut(s) 72, 180, 639, 648
NlaIII CATG 1 cut(s) 682
NlaIV GGNNCC 1 cut(s) 85
PctI GAATGC 1 cut(s) 565
PdmI GAANNNNTTC 2 cut(s) 165, 729
PfeI GAWTC 2 cut(s) 323, 618
PkrI GCNGC 3 cut(s) 259, 439, 712
PleI GAGTC 1 cut(s) 235
PpsI GAGTC 1 cut(s) 235
PspN4I GGNNCC 1 cut(s) 85
PsuI RGATCY 1 cut(s) 72
PvuII CAGCTG 1 cut(s) 257
RsaI GTAC 3 cut(s) 289, 534, 783
RsaNI GTAC 3 cut(s) 288, 533, 782
RseI CAYNNNNRTG 1 cut(s) 359
SatI GCNGC 3 cut(s) 258, 438, 711
Sau3AI GATC 4 cut(s) 72, 180, 639, 648
SchI GAGTC 1 cut(s) 235
SduI GDGCHC 1 cut(s) 634
SfaNI GCATC 2 cut(s) 543, 795
SmiMI CAYNNNNRTG 1 cut(s) 359
SmlI CTYRAG 1 cut(s) 243
SmoI CTYRAG 1 cut(s) 243
SpeI ACTAGT 1 cut(s) 659
Sse9I AATT 1 cut(s) 207
SspI AATATT 1 cut(s) 143
SspMI CTAG 4 cut(s) 266, 320, 660, 814
StyI CCWWGG 1 cut(s) 678
TaaI ACNGT 2 cut(s) 316, 369
TaqI TCGA 1 cut(s) 402
TasI AATT 1 cut(s) 207
TatI WGTACW 1 cut(s) 781
TfiI GAWTC 2 cut(s) 323, 618
TscAI CASTG 4 cut(s) 30, 47, 319, 477
TseI GCWGC 3 cut(s) 257, 437, 710
TspDTI ATGAA 4 cut(s) 77, 158, 669, 730
TspGWI ACGGA 1 cut(s) 520
TspRI CASTG 4 cut(s) 30, 47, 319, 477
XcmI CCANNNNNNNNNTGG 1 cut(s) 172
XmnI GAANNNNTTC 2 cut(s) 165, 729
XspI CTAG 4 cut(s) 266, 320, 660, 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.