MD05G1170200.v1.1

ER lumen protein retaining

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0015838
Physical Location & Seq
Forward (+)
29981902 .. 29984927
3026 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1170200.v1.1.491

Sequence Viewer

Length: 819 bp
ATGGGGAGGAGGAGGAGCTCGCCGGTGAACAAGCTGTTTCAATGGGTGAGGAGGCAGTCAATCAAAGTCAAGGTTTGTCTGGGTAGCCTGCTGGCATTGTGTGCTTTGGTGGCACTGAGGTTTTGGGTGAAGAATCACAACTACTTTTTCATTGCCTCTGAAGTCGTGCATGTCGTCGGGATCGTCGTCCTCATTTACAAGCTCACTACTCAGAAGACTTGCTCAGGTCTTTCCCTGAAGACTCAGGAACTCACAGCTCTCTTCCTAGCAGTAAGATTATTTTGCAGTACCATGATGGAGGGCGACATTCACACTGTGCTCGATTTTTCTACGCTCGTTTCAACAGTGTGGGTTATATACATGATACAGTTTAAATTGAAGTCAACCTACATCAATGAACTGGACAATTTTCGCTTATATTATGTGGTGGTGCCTGCTGCTGTCCTTGGCGTGCTTGTCCACCCGTATACACTATATAACCGCATCAGTCGAATCCTATGGGCATTTAGTGCATACATGGAAGCCGTTTCGGTACTGCCTCAACTCCGTTTGATGCAGAATGCGAAGATGATCGAACCATTTACCTCGCATTACGTATTTGCATTGGGCATTTCAAGGTTCTTGGCGTGTGCTCATTGGATTATTCAGATCTACGAGACTAGAGGAGGATATATATTTTTAGTCGGGAGCGGGTATTTCTGGATTTTGGCAGTTTTCCTCTGTGAGATGGTCCAAACATTCATCTTAGCTGACTTTTGTTACTACTACATAAAGAGTAAAATGGAAGGGCAGCTGGTAATGAGGATGCCTCCGGTCTAG

Protein Analysis

273

Amino Acids

31.44

Weight (kDa)

9.49

Isoelectric Point (pI)

41.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ER_lumen_recept PF00810 73 - 216 2.2e-43 ER lumen protein retaining receptor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015838)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19970 AT1G19970 AT3G25160
fragaria_vesca FvH4_2g18430
malus_domestica MD05G1170200.v1.1
prunus_persica Prupe.8G197400_v2.0.a1
pyrus_communis pycom05g15740
rosa_chinensis RchiOBHm_Chr6g0283911
rosa_laevigata RLG00000012787
rosa_rugosa Rorug06G0158400
rosa_samantha Rh6AG271400 Rh6BG272600 Rh6CG273800 Rh6DG266700
rosa_wichuraiana Rw6G023410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 430
AccBSI CCGCTC 1 cut(s) 690
AccI GTMKAC 1 cut(s) 467
AciI CCGC 2 cut(s) 481, 690
AclWI GGATC 1 cut(s) 188
AcuI CTGAAG 2 cut(s) 180, 257
AdeI CACNNNGTG 1 cut(s) 316
AfaI GTAC 2 cut(s) 289, 534
AgsI TTSAA 4 cut(s) 41, 342, 379, 615
AluBI AGCT 6 cut(s) 18, 34, 202, 257, 749, 793
AluI AGCT 6 cut(s) 18, 34, 202, 257, 749, 793
Alw21I GWGCWC 3 cut(s) 20, 321, 634
Alw26I GTCTC 1 cut(s) 650
AlwI GGATC 1 cut(s) 188
ApeKI GCWGC 2 cut(s) 437, 790
AspS9I GGNCC 1 cut(s) 730
AsuHPI GGTGA 3 cut(s) 37, 58, 139
AvaII GGWCC 1 cut(s) 730
BanI GGYRCC 1 cut(s) 430
BanII GRGCYC 1 cut(s) 20
BarI GAAGNNNNNNTAC 2 cut(s) 371, 403
BbsI GAAGAC 2 cut(s) 221, 245
Bbv12I GWGCWC 3 cut(s) 20, 321, 634
BbvI GCAGC 2 cut(s) 424, 802
BccI CCATC 2 cut(s) 289, 721
BceAI ACGGC 1 cut(s) 509
BcoDI GTCTC 1 cut(s) 650
BfaI CTAG 3 cut(s) 266, 660, 817
BglII AGATCT 1 cut(s) 648
BisI GCNGC 2 cut(s) 438, 791
BlsI GCNGC 2 cut(s) 439, 792
Bme18I GGWCC 1 cut(s) 730
BmgT120I GGNCC 1 cut(s) 730
BmiI GGNNCC 1 cut(s) 432
BmsI GCATC 3 cut(s) 492, 543, 795
BpiI GAAGAC 2 cut(s) 221, 245
BplI GAGNNNNNCTC 1 cut(s) 793
Bpu10I CCTNAGC 1 cut(s) 223
BsaAI YACGTR 1 cut(s) 595
BsaJI CCNNGG 1 cut(s) 445
BsaWI WCCGGW 1 cut(s) 811
Bse118I RCCGGY 1 cut(s) 22
Bse1I ACTGG 1 cut(s) 405
Bse3DI GCAATG 1 cut(s) 150
BseDI CCNNGG 1 cut(s) 445
BseGI GGATG 1 cut(s) 810
BseMI GCAATG 1 cut(s) 150
BseMII CTCAG 4 cut(s) 107, 224, 237, 257
BseNI ACTGG 1 cut(s) 405
BseRI GAGGAG 5 cut(s) 22, 25, 28, 64, 678
BseXI GCAGC 2 cut(s) 424, 802
BshNI GGYRCC 1 cut(s) 430
BsiHKAI GWGCWC 3 cut(s) 20, 321, 634
BsiSI CCGG 2 cut(s) 23, 812
BsmAI GTCTC 1 cut(s) 650
BsmI GAATGC 1 cut(s) 565
Bsp1286I GDGCHC 3 cut(s) 20, 321, 634
Bsp143I GATC 3 cut(s) 180, 570, 648
BspACI CCGC 2 cut(s) 481, 690
BspCNI CTCAG 4 cut(s) 108, 223, 236, 256
BspLI GGNNCC 1 cut(s) 432
BspPI GGATC 1 cut(s) 188
BspT107I GGYRCC 1 cut(s) 430
BsrBI CCGCTC 1 cut(s) 690
BsrDI GCAATG 1 cut(s) 150
BsrFI RCCGGY 1 cut(s) 22
BsrI ACTGG 1 cut(s) 405
BssAI RCCGGY 1 cut(s) 22
BssECI CCNNGG 1 cut(s) 445
BssMI GATC 3 cut(s) 180, 570, 648
BssNAI GTATAC 1 cut(s) 468
BssT1I CCWWGG 1 cut(s) 445
Bst1107I GTATAC 1 cut(s) 468
Bst4CI ACNGT 3 cut(s) 316, 346, 369
Bst6I CTCTTC 1 cut(s) 266
BstAPI GCANNNNNTGC 2 cut(s) 101, 509
BstBAI YACGTR 1 cut(s) 595
BstC8I GCNNGC 5 cut(s) 20, 89, 93, 435, 452
BstDEI CTNAG 5 cut(s) 116, 210, 223, 243, 745
BstF5I GGATG 1 cut(s) 810
BstKTI GATC 3 cut(s) 183, 573, 651
BstMAI GTCTC 1 cut(s) 650
BstMBI GATC 3 cut(s) 180, 570, 648
BstMWI GCNNNNNNNGC 3 cut(s) 101, 110, 509
BstNSI RCATGY 1 cut(s) 173
BstSNI TACGTA 1 cut(s) 595
BstV1I GCAGC 2 cut(s) 424, 802
BstV2I GAAGAC 2 cut(s) 221, 245
BstX2I RGATCY 1 cut(s) 648
BstYI RGATCY 1 cut(s) 648
BstZ17I GTATAC 1 cut(s) 468
BtsCI GGATG 1 cut(s) 810
BtsIMutI CAGTG 3 cut(s) 113, 312, 351
Cac8I GCNNGC 5 cut(s) 20, 89, 93, 435, 452
Cfr10I RCCGGY 1 cut(s) 22
Cfr13I GGNCC 1 cut(s) 730
Csp6I GTAC 2 cut(s) 288, 533
CviAII CATG 4 cut(s) 170, 292, 361, 517
CviJI RGCY 8 cut(s) 18, 34, 87, 202, 257, 524, 749, 793
CviKI_1 RGCY 8 cut(s) 18, 34, 87, 202, 257, 524, 749, 793
CviQI GTAC 2 cut(s) 288, 533
DdeI CTNAG 5 cut(s) 116, 210, 223, 243, 745
DpnI GATC 3 cut(s) 182, 572, 650
DpnII GATC 3 cut(s) 180, 570, 648
DraI TTTAAA 1 cut(s) 373
DraIII CACNNNGTG 1 cut(s) 316
Eam1104I CTCTTC 1 cut(s) 266
EarI CTCTTC 1 cut(s) 266
Ecl136II GAGCTC 1 cut(s) 18
Eco105I TACGTA 1 cut(s) 595
Eco130I CCWWGG 1 cut(s) 445
Eco24I GRGCYC 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 730
Eco53kI GAGCTC 1 cut(s) 18
Eco57I CTGAAG 2 cut(s) 180, 257
EcoICRI GAGCTC 1 cut(s) 18
EcoT14I CCWWGG 1 cut(s) 445
EcoT38I GRGCYC 1 cut(s) 20
ErhI CCWWGG 1 cut(s) 445
FaeI CATG 4 cut(s) 173, 295, 364, 520
FatI CATG 4 cut(s) 169, 291, 360, 516
FauI CCCGC 1 cut(s) 683
FblI GTMKAC 1 cut(s) 467
Fnu4HI GCNGC 2 cut(s) 438, 791
FriOI GRGCYC 1 cut(s) 20
Fsp4HI GCNGC 2 cut(s) 438, 791
FspBI CTAG 3 cut(s) 266, 660, 817
GluI GCNGC 2 cut(s) 438, 791
HapII CCGG 2 cut(s) 23, 812
Hin1II CATG 4 cut(s) 173, 295, 364, 520
HincII GTYRAC 1 cut(s) 384
HindII GTYRAC 1 cut(s) 384
HinfI GANTC 3 cut(s) 133, 241, 492
HpaII CCGG 2 cut(s) 23, 812
HphI GGTGA 3 cut(s) 37, 58, 139
Hpy166II GTNNAC 4 cut(s) 28, 384, 460, 468
Hpy188I TCNGA 3 cut(s) 160, 213, 648
Hpy188III TCNNGA 4 cut(s) 178, 245, 685, 700
Hpy8I GTNNAC 4 cut(s) 28, 384, 460, 468
Hpy99I CGWCG 2 cut(s) 179, 188
HpyAV CCTTC 1 cut(s) 779
HpyCH4III ACNGT 3 cut(s) 316, 346, 369
HpyCH4IV ACGT 1 cut(s) 594
HpyCH4V TGCA 5 cut(s) 169, 285, 512, 556, 602
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 110, 509
HpyF3I CTNAG 5 cut(s) 116, 210, 223, 243, 745
HpySE526I ACGT 1 cut(s) 594
Hsp92II CATG 4 cut(s) 173, 295, 364, 520
Kzo9I GATC 3 cut(s) 180, 570, 648
LmnI GCTCC 2 cut(s) 15, 687
Lsp1109I GCAGC 2 cut(s) 424, 802
LweI GCATC 3 cut(s) 492, 543, 795
MaeI CTAG 3 cut(s) 266, 660, 817
MaeII ACGT 1 cut(s) 594
MaeIII GTNAC 1 cut(s) 758
MalI GATC 3 cut(s) 182, 572, 650
MbiI CCGCTC 1 cut(s) 690
MboI GATC 3 cut(s) 180, 570, 648
MboII GAAGA 5 cut(s) 142, 226, 250, 253, 577
MflI RGATCY 1 cut(s) 648
MhlI GDGCHC 3 cut(s) 20, 321, 634
MluCI AATT 2 cut(s) 374, 406
MlyI GAGTC 1 cut(s) 235
MseI TTAA 1 cut(s) 372
MspA1I CMGCKG 1 cut(s) 793
MspI CCGG 2 cut(s) 23, 812
Mva1269I GAATGC 1 cut(s) 565
MwoI GCNNNNNNNGC 3 cut(s) 101, 110, 509
NdeII GATC 3 cut(s) 180, 570, 648
NlaIII CATG 4 cut(s) 173, 295, 364, 520
NlaIV GGNNCC 1 cut(s) 432
NspI RCATGY 1 cut(s) 173
PcsI WCGNNNNNNNCGW 3 cut(s) 171, 180, 183
PctI GAATGC 1 cut(s) 565
PfeI GAWTC 2 cut(s) 133, 492
PkrI GCNGC 2 cut(s) 439, 792
PleI GAGTC 1 cut(s) 235
PpsI GAGTC 1 cut(s) 235
Ppu21I YACGTR 1 cut(s) 595
Psp124BI GAGCTC 1 cut(s) 20
PspN4I GGNNCC 1 cut(s) 432
PspPI GGNCC 1 cut(s) 730
PsuI RGATCY 1 cut(s) 648
PvuII CAGCTG 1 cut(s) 793
RsaI GTAC 2 cut(s) 289, 534
RsaNI GTAC 2 cut(s) 288, 533
SacI GAGCTC 1 cut(s) 20
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 2 cut(s) 438, 791
Sau3AI GATC 3 cut(s) 180, 570, 648
Sau96I GGNCC 1 cut(s) 730
SchI GAGTC 1 cut(s) 235
SduI GDGCHC 3 cut(s) 20, 321, 634
SfaNI GCATC 3 cut(s) 492, 543, 795
SgrAI CRCCGGYG 1 cut(s) 22
SinI GGWCC 1 cut(s) 730
SnaBI TACGTA 1 cut(s) 595
Sse9I AATT 2 cut(s) 374, 406
SsiI CCGC 2 cut(s) 481, 690
SspMI CTAG 3 cut(s) 266, 660, 817
SstI GAGCTC 1 cut(s) 20
StyI CCWWGG 1 cut(s) 445
TaaI ACNGT 3 cut(s) 316, 346, 369
TaiI ACGT 1 cut(s) 597
TaqI TCGA 3 cut(s) 321, 490, 573
TasI AATT 2 cut(s) 374, 406
TfiI GAWTC 2 cut(s) 133, 492
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TscAI CASTG 3 cut(s) 120, 319, 351
TseI GCWGC 2 cut(s) 437, 790
TspDTI ATGAA 3 cut(s) 139, 411, 730
TspGWI ACGGA 1 cut(s) 536
TspRI CASTG 3 cut(s) 120, 319, 351
VpaK11BI GGWCC 1 cut(s) 730
XceI RCATGY 1 cut(s) 173
XmiI GTMKAC 1 cut(s) 467
XspI CTAG 3 cut(s) 266, 660, 817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.