AT3G25590

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014221
Physical Location & Seq
Forward (+)
9301747 .. 9304088
2342 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G25590.1

Sequence Viewer

Length: 1272 bp
ATGAAAGAAAGAGGAAAGAGAACAGCAATGGAACGAAGAAACGACGACGTTTCGTTATATTACAGTACTCCGTCGGAATTCACTTGCCGGAAACATCCATCTGTTTCCTCCGGCGTTGGTATTTGTCCGTACTGTCTCAACGACAGATTAGTCAATCTCGTATGCTCCGAATGCGGCGAGCAGCGACTCTCTTCTTGCTCGTGTTCTGATATCTCTCCTACTCGAACCGTAGATGCCGCCGTAGACGCCGCCGTCGGAGAAAACGTTGTCAGGATCTCTTCTCTCATCGACGAAGAGAGGGCGAAACAGAGGAAAGAGACGAAACAGAGGAAAACAGAGGAAGTGGTAGTGTTCAAGAGGAGTAGTAGCAGTTGTGTTGAGATTAATAAGAGGACGAAGAATCATAGATTCTCGAGAATCGGTAGGTTTTTCAGGAAGATTAATCTTAAAAAAGAGAGAGATTTTGAGAAGAACAATAATAATGATTCATGGGTTTTGGATTATAACAATGATGTGAAGAAACTAGGAGTTTCGAGATCGAGATCGCTTTGTAGCTTTCGAGGAAAAGATTTGTATTGTATCGGATCAGAAGAAGACGGTTCTTCGTATTCGGGTGCTTTCTCCGCCGCGAGGAGCTCAAGTGTTAATGGCGGATTAGGGTTATGCGAAACAGAGTATTCTCGGAAGAGTAACTTCGAAGGAAGGAAGAGCAACTTCAGCGAGACGACGGAGCATTGGAAGAGTAATTTCGAAGGAGGAAGGAAGAGTAATTTCAGTGAGACGACGACGGAGAATCGGAAGAGTAATTTCAGTGAATCGGAGCCGCCGAGAAGAAGCGGTTTCGAGGCTAGGAAGAGTAATTTCAGTGAAACAGAGTATCCTACTAGGAGGAGTAATTTTAGTGAAACAGAGTATAATACTCGAAGAGGAAACAATCCGGCGACGGCGGAGAATCATCCACGGAGGAGTAGTAATTACGAAGCAGCGAGGAAAAGCGATTCGGCGGCGATGAATTTTACGAGAAGGGTAATGTCGATGAAAGAGAGTAGCTACTTCACCGGAGGAGAGGAGCCTGGTTTCATTGATCTTAAGTTTGATTCTTCCGGTGGAGGAGGAGGAGATGTAAACGACGGCGTTTTGGAACACGGAGGAGGAGGATCTTGTCGGAAAGATGGAGGAGAGTTGCGTAAGAGTCGAAAGAGTTTCAAAGGATGGAAGTGGATATTTGGACATCATCAACATCAACTTCATCATCATAACAGAGATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

423

Amino Acids

47.81

Weight (kDa)

9.3

Isoelectric Point (pI)

55.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014221)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25590
fragaria_vesca FvH4_4g28580
malus_domestica MD13G1052200.v1.1 MD16G1057700.v1.1
prunus_persica Prupe.1G299900_v2.0.a1
pyrus_communis pycom13g04650 pycom16g05050
rosa_chinensis RchiOBHm_Chr4g0436671
rosa_laevigata RLG00000006505
rosa_multiflora Rmu_sc0003130.1_g000019
rosa_roxburghii Rroxscaffold_5G00377750
rosa_rugosa Rorug04G0295100
rosa_samantha Rh4AG349600 Rh4BG358300 Rh4CG372700 Rh4DG352500
rosa_wichuraiana Rw4G030580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 504
AasI GACNNNNNNGTC 2 cut(s) 149, 251
AccI GTMKAC 1 cut(s) 243
AccII CGCG 1 cut(s) 629
AclI AACGTT 1 cut(s) 264
AclWI GGATC 3 cut(s) 281, 592, 1165
AcsI RAATTY 2 cut(s) 77, 1012
AcuI CTGAAG 1 cut(s) 700
AcyI GRCGYC 1 cut(s) 246
AfaI GTAC 2 cut(s) 67, 131
AfiI CCNNNNNNNGG 1 cut(s) 630
AflII CTTAAG 1 cut(s) 1088
AgsI TTSAA 2 cut(s) 355, 1207
AjnI CCWGG 1 cut(s) 1072
AluBI AGCT 3 cut(s) 555, 636, 1050
AluI AGCT 3 cut(s) 555, 636, 1050
Alw21I GWGCWC 1 cut(s) 638
Alw26I GTCTC 4 cut(s) 140, 311, 716, 773
AlwI GGATC 3 cut(s) 281, 592, 1165
Ama87I CYCGRG 1 cut(s) 412
ApeKI GCWGC 2 cut(s) 181, 983
ApoI RAATTY 2 cut(s) 77, 1012
AseI ATTAAT 2 cut(s) 384, 441
AsuHPI GGTGA 1 cut(s) 1048
AsuII TTCGAA 2 cut(s) 696, 750
AvaI CYCGRG 1 cut(s) 412
BanII GRGCYC 1 cut(s) 638
BauI CACGAG 1 cut(s) 199
BbsI GAAGAC 1 cut(s) 600
Bbv12I GWGCWC 1 cut(s) 638
BbvI GCAGC 2 cut(s) 193, 995
BccI CCATC 3 cut(s) 106, 1166, 1206
BceAI ACGGC 4 cut(s) 224, 236, 960, 1147
BciT130I CCWGG 1 cut(s) 1074
BciVI GTATCC 1 cut(s) 888
BcoDI GTCTC 4 cut(s) 140, 311, 716, 773
BfaI CTAG 3 cut(s) 524, 849, 885
BfrI CTTAAG 1 cut(s) 1088
BfuI GTATCC 1 cut(s) 888
BisI GCNGC 8 cut(s) 175, 182, 237, 249, 627, 824, 984, 1005
BlsI GCNGC 8 cut(s) 176, 183, 238, 250, 628, 825, 985, 1006
BmcAI AGTACT 1 cut(s) 67
Bme1390I CCNGG 1 cut(s) 1074
BmeT110I CYCGRG 1 cut(s) 412
BmiI GGNNCC 2 cut(s) 822, 1071
BmrFI CCNGG 1 cut(s) 1074
BmsI GCATC 1 cut(s) 223
BpiI GAAGAC 1 cut(s) 600
Bpu14I TTCGAA 2 cut(s) 696, 750
BpuEI CTTGAG 1 cut(s) 622
BsaBI GATNNNNATC 1 cut(s) 541
BsaHI GRCGYC 1 cut(s) 246
BsaJI CCNNGG 1 cut(s) 959
BsaWI WCCGGW 2 cut(s) 1058, 1103
BsaXI ACNNNNNCTCC 8 cut(s) 249, 279, 625, 655, 955, 985, 1107, 1137
Bsc4I CCNNNNNNNGG 1 cut(s) 630
Bse3DI GCAATG 1 cut(s) 33
Bse8I GATNNNNATC 1 cut(s) 541
BseBI CCWGG 1 cut(s) 1074
BseDI CCNNGG 1 cut(s) 959
BseGI GGATG 3 cut(s) 94, 955, 1217
BseJI GATNNNNATC 1 cut(s) 541
BseLI CCNNNNNNNGG 1 cut(s) 630
BseMI GCAATG 1 cut(s) 33
BseXI GCAGC 2 cut(s) 193, 995
Bsh1236I CGCG 1 cut(s) 629
BsiHKAI GWGCWC 1 cut(s) 638
BsiHKCI CYCGRG 1 cut(s) 412
BsiSI CCGG 5 cut(s) 88, 111, 938, 1059, 1104
BslI CCNNNNNNNGG 1 cut(s) 630
BsmAI GTCTC 4 cut(s) 140, 311, 716, 773
BsmBI CGTCTC 3 cut(s) 311, 716, 773
BsmI GAATGC 1 cut(s) 176
BsoBI CYCGRG 1 cut(s) 412
Bsp119I TTCGAA 2 cut(s) 696, 750
Bsp1286I GDGCHC 1 cut(s) 638
Bsp143I GATC 6 cut(s) 273, 536, 542, 584, 1084, 1157
BspFNI CGCG 1 cut(s) 629
BspHI TCATGA 1 cut(s) 1268
BspLI GGNNCC 2 cut(s) 822, 1071
BspPI GGATC 3 cut(s) 281, 592, 1165
BspQI GCTCTTC 1 cut(s) 701
BspT104I TTCGAA 2 cut(s) 696, 750
BspTI CTTAAG 1 cut(s) 1088
BsrDI GCAATG 1 cut(s) 33
BssECI CCNNGG 1 cut(s) 959
BssMI GATC 6 cut(s) 273, 536, 542, 584, 1084, 1157
BssNI GRCGYC 1 cut(s) 246
BssSI CACGAG 1 cut(s) 199
Bst2BI CACGAG 1 cut(s) 199
Bst2UI CCWGG 1 cut(s) 1074
Bst4CI ACNGT 4 cut(s) 65, 134, 229, 599
BstACI GRCGYC 1 cut(s) 246
BstAFI CTTAAG 1 cut(s) 1088
BstBI TTCGAA 2 cut(s) 696, 750
BstC8I GCNNGC 1 cut(s) 179
BstDSI CCRYGG 1 cut(s) 959
BstF5I GGATG 3 cut(s) 94, 955, 1217
BstFNI CGCG 1 cut(s) 629
BstKTI GATC 6 cut(s) 276, 539, 545, 587, 1087, 1160
BstMAI GTCTC 4 cut(s) 140, 311, 716, 773
BstMBI GATC 6 cut(s) 273, 536, 542, 584, 1084, 1157
BstMWI GCNNNNNNNGC 4 cut(s) 171, 245, 623, 717
BstNI CCWGG 1 cut(s) 1074
BstSCI CCNGG 1 cut(s) 1072
BstUI CGCG 1 cut(s) 629
BstV1I GCAGC 2 cut(s) 193, 995
BstV2I GAAGAC 1 cut(s) 600
BstX2I RGATCY 2 cut(s) 273, 1157
BstYI RGATCY 2 cut(s) 273, 1157
BsuI GTATCC 1 cut(s) 888
BtgI CCRYGG 1 cut(s) 959
BtgZI GCGATG 1 cut(s) 1022
BtsCI GGATG 3 cut(s) 94, 955, 1217
BtsIMutI CAGTG 3 cut(s) 781, 817, 871
Cac8I GCNNGC 1 cut(s) 179
CciI TCATGA 1 cut(s) 1268
CseI GACGC 1 cut(s) 254
Csp6I GTAC 2 cut(s) 66, 130
CviAII CATG 2 cut(s) 489, 1269
CviJI RGCY 6 cut(s) 555, 636, 823, 848, 1050, 1072
CviKI_1 RGCY 6 cut(s) 555, 636, 823, 848, 1050, 1072
CviQI GTAC 2 cut(s) 66, 130
DpnI GATC 6 cut(s) 275, 538, 544, 586, 1086, 1159
DpnII GATC 6 cut(s) 273, 536, 542, 584, 1084, 1157
DrdI GACNNNNNNGTC 2 cut(s) 149, 251
DseDI GACNNNNNNGTC 2 cut(s) 149, 251
EciI GGCGGA 3 cut(s) 613, 666, 962
Ecl136II GAGCTC 1 cut(s) 636
Eco24I GRGCYC 1 cut(s) 638
Eco32I GATATC 1 cut(s) 211
Eco53kI GAGCTC 1 cut(s) 636
Eco57I CTGAAG 1 cut(s) 700
Eco88I CYCGRG 1 cut(s) 412
EcoICRI GAGCTC 1 cut(s) 636
EcoRI GAATTC 1 cut(s) 77
EcoRII CCWGG 1 cut(s) 1072
EcoRV GATATC 1 cut(s) 211
EcoT38I GRGCYC 1 cut(s) 638
Esp3I CGTCTC 3 cut(s) 311, 716, 773
FaeI CATG 2 cut(s) 492, 1272
FaiI YATR 9 cut(s) 58, 163, 405, 490, 504, 664, 915, 1257, 1270
FalI AAGNNNNNCTT 4 cut(s) 677, 709, 698, 730
FatI CATG 2 cut(s) 488, 1268
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 8 cut(s) 175, 182, 237, 249, 627, 824, 984, 1005
FokI GGATG 3 cut(s) 81, 942, 1224
FriOI GRGCYC 1 cut(s) 638
Fsp4HI GCNGC 8 cut(s) 175, 182, 237, 249, 627, 824, 984, 1005
FspBI CTAG 3 cut(s) 524, 849, 885
GluI GCNGC 8 cut(s) 175, 182, 237, 249, 627, 824, 984, 1005
HapII CCGG 5 cut(s) 88, 111, 938, 1059, 1104
HgaI GACGC 1 cut(s) 254
Hin1I GRCGYC 1 cut(s) 246
Hin1II CATG 2 cut(s) 492, 1272
HpaII CCGG 5 cut(s) 88, 111, 938, 1059, 1104
HphI GGTGA 1 cut(s) 1048
Hpy166II GTNNAC 2 cut(s) 244, 1126
Hpy188III TCNNGA 8 cut(s) 271, 355, 412, 414, 433, 534, 540, 1269
Hpy8I GTNNAC 2 cut(s) 244, 1126
HpyAV CCTTC 5 cut(s) 692, 696, 746, 753, 1017
HpyCH4III ACNGT 4 cut(s) 65, 134, 229, 599
HpyCH4IV ACGT 2 cut(s) 48, 264
HpyF10VI GCNNNNNNNGC 4 cut(s) 171, 245, 623, 717
HpySE526I ACGT 2 cut(s) 48, 264
Hsp92I GRCGYC 1 cut(s) 246
Hsp92II CATG 2 cut(s) 492, 1272
Kzo9I GATC 6 cut(s) 273, 536, 542, 584, 1084, 1157
LguI GCTCTTC 1 cut(s) 701
LmnI GCTCC 5 cut(s) 170, 633, 730, 820, 1069
LpnPI CCDG 9 cut(s) 101, 124, 256, 418, 951, 1059, 1072, 1086, 1117
Lsp1109I GCAGC 2 cut(s) 193, 995
LweI GCATC 1 cut(s) 223
MaeI CTAG 3 cut(s) 524, 849, 885
MaeII ACGT 2 cut(s) 48, 264
MaeIII GTNAC 1 cut(s) 689
MalI GATC 6 cut(s) 275, 538, 544, 586, 1086, 1159
MboI GATC 6 cut(s) 273, 536, 542, 584, 1084, 1157
MflI RGATCY 2 cut(s) 273, 1157
MhlI GDGCHC 1 cut(s) 638
MluCI AATT 8 cut(s) 77, 745, 769, 805, 859, 895, 973, 1012
MlyI GAGTC 2 cut(s) 180, 1201
MmeI TCCRAC 3 cut(s) 54, 235, 1145
MseI TTAA 5 cut(s) 384, 441, 447, 645, 1089
MspCI CTTAAG 1 cut(s) 1088
MspI CCGG 5 cut(s) 88, 111, 938, 1059, 1104
MspR9I CCNGG 1 cut(s) 1074
Mva1269I GAATGC 1 cut(s) 176
MvaI CCWGG 1 cut(s) 1074
MvnI CGCG 1 cut(s) 629
MwoI GCNNNNNNNGC 4 cut(s) 171, 245, 623, 717
NdeII GATC 6 cut(s) 273, 536, 542, 584, 1084, 1157
NlaIII CATG 2 cut(s) 492, 1272
NlaIV GGNNCC 2 cut(s) 822, 1071
NmeAIII GCCGAG 1 cut(s) 852
NspV TTCGAA 2 cut(s) 696, 750
PaeR7I CTCGAG 1 cut(s) 412
PagI TCATGA 1 cut(s) 1268
PciSI GCTCTTC 1 cut(s) 701
PcsI WCGNNNNNNNCGW 3 cut(s) 165, 261, 824
PctI GAATGC 1 cut(s) 176
PkrI GCNGC 8 cut(s) 176, 183, 238, 250, 628, 825, 985, 1006
PleI GAGTC 2 cut(s) 180, 1200
PpsI GAGTC 2 cut(s) 180, 1200
PshBI ATTAAT 2 cut(s) 384, 441
PsiI TTATAA 1 cut(s) 504
Psp124BI GAGCTC 1 cut(s) 638
Psp1406I AACGTT 1 cut(s) 264
Psp6I CCWGG 1 cut(s) 1072
PspGI CCWGG 1 cut(s) 1072
PspN4I GGNNCC 2 cut(s) 822, 1071
PsuI RGATCY 2 cut(s) 273, 1157
RsaI GTAC 2 cut(s) 67, 131
RsaNI GTAC 2 cut(s) 66, 130
SacI GAGCTC 1 cut(s) 638
SapI GCTCTTC 1 cut(s) 701
SaqAI TTAA 5 cut(s) 384, 441, 447, 645, 1089
SatI GCNGC 8 cut(s) 175, 182, 237, 249, 627, 824, 984, 1005
Sau3AI GATC 6 cut(s) 273, 536, 542, 584, 1084, 1157
ScaI AGTACT 1 cut(s) 67
SchI GAGTC 2 cut(s) 180, 1201
ScrFI CCNGG 1 cut(s) 1074
SduI GDGCHC 1 cut(s) 638
SetI ASST 6 cut(s) 51, 267, 428, 557, 638, 1052
SfaNI GCATC 1 cut(s) 223
Sfr274I CTCGAG 1 cut(s) 412
SfuI TTCGAA 2 cut(s) 696, 750
SlaI CTCGAG 1 cut(s) 412
SmlI CTYRAG 3 cut(s) 412, 637, 1088
SmoI CTYRAG 3 cut(s) 412, 637, 1088
Sse9I AATT 8 cut(s) 77, 745, 769, 805, 859, 895, 973, 1012
SspMI CTAG 3 cut(s) 524, 849, 885
SstI GAGCTC 1 cut(s) 638
StyD4I CCNGG 1 cut(s) 1072
TaaI ACNGT 4 cut(s) 65, 134, 229, 599
TaiI ACGT 2 cut(s) 51, 267
TasI AATT 8 cut(s) 77, 745, 769, 805, 859, 895, 973, 1012
TatI WGTACW 1 cut(s) 65
TauI GCSGC 6 cut(s) 177, 239, 251, 629, 826, 1007
Tru1I TTAA 5 cut(s) 384, 441, 447, 645, 1089
Tru9I TTAA 5 cut(s) 384, 441, 447, 645, 1089
TscAI CASTG 3 cut(s) 781, 817, 871
TseI GCWGC 2 cut(s) 181, 983
TspDTI ATGAA 7 cut(s) 17, 477, 1025, 1052, 1069, 1238, 1257
TspGWI ACGGA 6 cut(s) 60, 117, 743, 803, 976, 1161
TspRI CASTG 3 cut(s) 781, 817, 871
Vha464I CTTAAG 1 cut(s) 1088
VspI ATTAAT 2 cut(s) 384, 441
XapI RAATTY 2 cut(s) 77, 1012
XhoI CTCGAG 1 cut(s) 412
XmiI GTMKAC 1 cut(s) 243
XspI CTAG 3 cut(s) 524, 849, 885
ZrmI AGTACT 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.