MD13G1052200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014221
Physical Location & Seq
Reverse (-)
3698342 .. 3699511
1170 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1052200.v1.1.491

Sequence Viewer

Length: 1170 bp
ATGAAAGAGAGGGGCAAAGCTGTAGAAGCAGCTCGCGACAATATCGACATGGAGTACTACTCTTCGTCTGAAATGCCGTGTCGAAGGCACCCCAATTCGTCTTCTGTCGGGATATGCGCATTCTGTCTGAAAGATCGTCTGCTCCAGCTGGCTTGCTCCGACTGCGGCGAGCAGCGCCTCTCCTCCTGCTCCTGCTCCGCCGTCTCGTCTAACCGCAACTCCACTGTGGAGGTCGGCAGCGTCGGTAGAGTCTCGTTTCTGATCGAGAACGAAAAGCAGGAGCCTTTACAAAAACCCAGCAAAACCCAAGAGAAAGAGGACGTGGTGGTGCTCCGGCGAAGCAGCAGCAGTTGTGTCGAGATCAAGAAAAGTAATTTTTGGAGATTTGGGAGGTTTTTTCGGAAGAAACGATCGGAAAAAGATGGTGGGAATAAGAGTGTTGGTGGGTTTGATGAGAAAAGTGAGATGTGGCTGGTTGATCACATGGGAGTTTCGAGATCAAGGTCTCTGTGCAGTTTCAGAGGCGGGGCCCTCTTCGGATCGGAGGATGGCGGCGAGCATTTGATGGTTTCCGGTGCCCGGAGCTCCATTTCTGCTGCGAGAAGCTCTAGCGTTACAACTGCCGGAATGGTTTTGGATTCCGGGAGAAAGAGCGGATTTAGTGAGGGGAGAAAGAGTGGGTTTAGTGAGGGAAGAAAGAGTGGATTTAGTGAAGGGAGAAGGAGTGGATTTAGTGAGGCAGAGCCGAGAAAAAGCGGTTTTGATGGTGAGAAGAAAGAAGATGTTGTAGGGTTTAGCAGTGCAACGAAACGCGTTTTTTCGCTGAAAGAGAGTGATTTCGGCATGGATGAATCGAGGTTTATCGATTTGAAGCTCGATTTCGCGGCGGATTCAAAGCCGGAGTTTTCGGGTTTGAGGGTGGGGAGCATTGCAGATGGAGAGACTGTTTTTGGGGGCATGAGAAGGAGTGAGTTTGTGGGGGATGAATGCGATGGGGGTTCGTTTGGTATTTTCAGACACGGTGGTTCATGTAGATTGACAGTGAATGAGAGAGGGATAAAGAGGGGGAGCAAAAGTTTGAAGGGTTGGAGGTGGATTTTCAGGCATCATCCAATTCCAAGCTGGGGAAGTAGTAGGAAGAAGGATGAAGATTTAATGTTCAAAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

390

Amino Acids

42.88

Weight (kDa)

9.25

Isoelectric Point (pI)

58.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014221)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25590
fragaria_vesca FvH4_4g28580
malus_domestica MD13G1052200.v1.1 MD16G1057700.v1.1
prunus_persica Prupe.1G299900_v2.0.a1
pyrus_communis pycom13g04650 pycom16g05050
rosa_chinensis RchiOBHm_Chr4g0436671
rosa_laevigata RLG00000006505
rosa_multiflora Rmu_sc0003130.1_g000019
rosa_roxburghii Rroxscaffold_5G00377750
rosa_rugosa Rorug04G0295100
rosa_samantha Rh4AG349600 Rh4BG358300 Rh4CG372700 Rh4DG352500
rosa_wichuraiana Rw4G030580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 118
AccB1I GGYRCC 2 cut(s) 87, 575
AccBSI CCGCTC 1 cut(s) 654
AccII CGCG 3 cut(s) 36, 813, 884
AciI CCGC 9 cut(s) 165, 198, 214, 525, 552, 654, 756, 884, 887
AclWI GGATC 1 cut(s) 547
AfaI GTAC 1 cut(s) 56
AfiI CCNNNNNNNGG 2 cut(s) 579, 1124
AflIII ACRYGT 1 cut(s) 811
AgsI TTSAA 4 cut(s) 871, 894, 1081, 1162
AjiI CACGTC 1 cut(s) 322
AluBI AGCT 7 cut(s) 20, 32, 148, 585, 606, 874, 1122
AluI AGCT 7 cut(s) 20, 32, 148, 585, 606, 874, 1122
Alw21I GWGCWC 2 cut(s) 333, 587
Alw26I GTCTC 4 cut(s) 208, 256, 510, 935
AlwI GGATC 1 cut(s) 547
AoxI GGCC 1 cut(s) 528
ApaI GGGCCC 1 cut(s) 532
ApeKI GCWGC 6 cut(s) 29, 172, 237, 342, 345, 596
AspLEI GCGC 2 cut(s) 119, 177
AspS9I GGNCC 2 cut(s) 528, 529
AsuC2I CCSGG 2 cut(s) 580, 643
AsuHPI GGTGA 1 cut(s) 779
BaeGI GKGCMC 2 cut(s) 532, 580
BanI GGYRCC 2 cut(s) 87, 575
BanII GRGCYC 2 cut(s) 532, 587
BbsI GAAGAC 1 cut(s) 93
Bbv12I GWGCWC 2 cut(s) 333, 587
BbvI GCAGC 6 cut(s) 41, 184, 249, 354, 357, 583
BccI CCATC 6 cut(s) 416, 542, 559, 758, 929, 986
BceAI ACGGC 2 cut(s) 61, 185
BcgI CGANNNNNNTGC 2 cut(s) 337, 371
BclI TGATCA 1 cut(s) 478
BcnI CCSGG 2 cut(s) 580, 643
BcoDI GTCTC 4 cut(s) 208, 256, 510, 935
BfaI CTAG 1 cut(s) 609
BfmI CTRYAG 1 cut(s) 21
BfoI RGCGCY 1 cut(s) 178
BisI GCNGC 9 cut(s) 30, 166, 173, 238, 343, 346, 553, 597, 885
BlsI GCNGC 9 cut(s) 31, 167, 174, 239, 344, 347, 554, 598, 886
BmcAI AGTACT 1 cut(s) 56
Bme1390I CCNGG 2 cut(s) 580, 643
BmgBI CACGTC 1 cut(s) 322
BmgT120I GGNCC 2 cut(s) 528, 529
BmiI GGNNCC 5 cut(s) 89, 282, 529, 530, 577
BmrFI CCNGG 2 cut(s) 580, 643
BmsI GCATC 1 cut(s) 1114
BpiI GAAGAC 1 cut(s) 93
BplI GAGNNNNNCTC 2 cut(s) 44, 76
BpmI CTGGAG 1 cut(s) 128
BpuMI CCSGG 2 cut(s) 580, 643
Bsa29I ATCGAT 1 cut(s) 864
BsaI GGTCTC 1 cut(s) 510
BsaWI WCCGGW 1 cut(s) 572
Bsc4I CCNNNNNNNGG 2 cut(s) 579, 1124
Bse3DI GCAATG 1 cut(s) 927
BseCI ATCGAT 1 cut(s) 864
BseGI GGATG 5 cut(s) 553, 853, 988, 1108, 1150
BseLI CCNNNNNNNGG 2 cut(s) 579, 1124
BseMI GCAATG 1 cut(s) 927
BseRI GAGGAG 1 cut(s) 172
BseSI GKGCMC 2 cut(s) 532, 580
BseXI GCAGC 6 cut(s) 41, 184, 249, 354, 357, 583
BseYI CCCAGC 2 cut(s) 296, 1122
BsgI GTGCAG 1 cut(s) 532
Bsh1236I CGCG 3 cut(s) 36, 813, 884
Bsh1285I CGRYCG 1 cut(s) 413
BshFI GGCC 1 cut(s) 530
BshNI GGYRCC 2 cut(s) 87, 575
BshVI ATCGAT 1 cut(s) 864
BsiEI CGRYCG 1 cut(s) 413
BsiHKAI GWGCWC 2 cut(s) 333, 587
BsiSI CCGG 6 cut(s) 334, 573, 580, 624, 642, 899
BslI CCNNNNNNNGG 2 cut(s) 579, 1124
BsmAI GTCTC 4 cut(s) 208, 256, 510, 935
BsmBI CGTCTC 1 cut(s) 208
BsmI GAATGC 2 cut(s) 119, 992
BsnI GGCC 1 cut(s) 530
Bso31I GGTCTC 1 cut(s) 510
Bsp120I GGGCCC 1 cut(s) 528
Bsp1286I GDGCHC 4 cut(s) 333, 532, 580, 587
Bsp143I GATC 7 cut(s) 133, 261, 360, 410, 478, 497, 539
Bsp68I TCGCGA 1 cut(s) 36
BspACI CCGC 9 cut(s) 165, 198, 214, 525, 552, 654, 756, 884, 887
BspANI GGCC 1 cut(s) 530
BspDI ATCGAT 1 cut(s) 864
BspFNI CGCG 3 cut(s) 36, 813, 884
BspLI GGNNCC 5 cut(s) 89, 282, 529, 530, 577
BspPI GGATC 1 cut(s) 547
BspT107I GGYRCC 2 cut(s) 87, 575
BspTNI GGTCTC 1 cut(s) 510
BsrBI CCGCTC 1 cut(s) 654
BsrDI GCAATG 1 cut(s) 927
BssMI GATC 7 cut(s) 133, 261, 360, 410, 478, 497, 539
Bst4CI ACNGT 4 cut(s) 226, 946, 1022, 1042
Bst6I CTCTTC 2 cut(s) 67, 539
BstC8I GCNNGC 5 cut(s) 34, 150, 154, 170, 557
BstF5I GGATG 5 cut(s) 553, 853, 988, 1108, 1150
BstFNI CGCG 3 cut(s) 36, 813, 884
BstH2I RGCGCY 1 cut(s) 178
BstHHI GCGC 2 cut(s) 119, 177
BstKTI GATC 7 cut(s) 136, 264, 363, 413, 481, 500, 542
BstMAI GTCTC 4 cut(s) 208, 256, 510, 935
BstMBI GATC 7 cut(s) 133, 261, 360, 410, 478, 497, 539
BstMCI CGRYCG 1 cut(s) 413
BstMWI GCNNNNNNNGC 3 cut(s) 26, 162, 174
BstSCI CCNGG 2 cut(s) 578, 641
BstSFI CTRYAG 1 cut(s) 21
BstSLI GKGCMC 2 cut(s) 532, 580
BstUI CGCG 3 cut(s) 36, 813, 884
BstV1I GCAGC 6 cut(s) 41, 184, 249, 354, 357, 583
BstV2I GAAGAC 1 cut(s) 93
Bsu15I ATCGAT 1 cut(s) 864
BsuRI GGCC 1 cut(s) 530
BsuTUI ATCGAT 1 cut(s) 864
BtgZI GCGATG 1 cut(s) 1005
BtrI CACGTC 1 cut(s) 322
BtsCI GGATG 5 cut(s) 553, 853, 988, 1108, 1150
BtsI GCAGTG 1 cut(s) 805
BtsIMutI CAGTG 3 cut(s) 222, 805, 1047
BtuMI TCGCGA 1 cut(s) 36
Cac8I GCNNGC 5 cut(s) 34, 150, 154, 170, 557
CfoI GCGC 2 cut(s) 119, 177
Cfr13I GGNCC 2 cut(s) 528, 529
ClaI ATCGAT 1 cut(s) 864
CseI GACGC 1 cut(s) 229
Csp6I GTAC 1 cut(s) 55
CviAII CATG 5 cut(s) 49, 484, 844, 958, 1029
CviQI GTAC 1 cut(s) 55
DpnI GATC 7 cut(s) 135, 263, 362, 412, 480, 499, 541
DpnII GATC 7 cut(s) 133, 261, 360, 410, 478, 497, 539
Eam1104I CTCTTC 2 cut(s) 67, 539
EarI CTCTTC 2 cut(s) 67, 539
EciI GGCGGA 2 cut(s) 187, 902
Ecl136II GAGCTC 1 cut(s) 585
Eco24I GRGCYC 2 cut(s) 532, 587
Eco31I GGTCTC 1 cut(s) 510
Eco53kI GAGCTC 1 cut(s) 585
EcoICRI GAGCTC 1 cut(s) 585
EcoO109I RGGNCCY 2 cut(s) 528, 529
EcoT38I GRGCYC 2 cut(s) 532, 587
Esp3I CGTCTC 1 cut(s) 208
FaeI CATG 5 cut(s) 52, 487, 847, 961, 1032
FaiI YATR 6 cut(s) 50, 115, 485, 845, 959, 1030
FatI CATG 5 cut(s) 48, 483, 843, 957, 1028
FauI CCCGC 1 cut(s) 518
FbaI TGATCA 1 cut(s) 478
Fnu4HI GCNGC 9 cut(s) 30, 166, 173, 238, 343, 346, 553, 597, 885
FokI GGATG 5 cut(s) 560, 860, 995, 1095, 1157
FriOI GRGCYC 2 cut(s) 532, 587
Fsp4HI GCNGC 9 cut(s) 30, 166, 173, 238, 343, 346, 553, 597, 885
FspAI RTGCGCAY 1 cut(s) 118
FspBI CTAG 1 cut(s) 609
FspI TGCGCA 1 cut(s) 118
GlaI GCGC 2 cut(s) 118, 176
GluI GCNGC 9 cut(s) 30, 166, 173, 238, 343, 346, 553, 597, 885
GsaI CCCAGC 2 cut(s) 300, 1126
GsuI CTGGAG 1 cut(s) 128
HaeII RGCGCY 1 cut(s) 178
HaeIII GGCC 1 cut(s) 530
HapII CCGG 6 cut(s) 334, 573, 580, 624, 642, 899
HgaI GACGC 1 cut(s) 229
HhaI GCGC 2 cut(s) 119, 177
Hin1II CATG 5 cut(s) 52, 487, 847, 961, 1032
Hin6I GCGC 2 cut(s) 117, 175
HinP1I GCGC 2 cut(s) 117, 175
HinfI GANTC 4 cut(s) 249, 638, 851, 890
HpaII CCGG 6 cut(s) 334, 573, 580, 624, 642, 899
HphI GGTGA 1 cut(s) 779
Hpy188III TCNNGA 6 cut(s) 35, 109, 265, 358, 364, 495
Hpy99I CGWCG 1 cut(s) 245
HpyAV CCTTC 6 cut(s) 78, 707, 714, 957, 1075, 1135
HpyCH4III ACNGT 4 cut(s) 226, 946, 1022, 1042
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 3 cut(s) 513, 803, 932
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 162, 174
HpySE526I ACGT 1 cut(s) 321
Hsp92II CATG 5 cut(s) 52, 487, 847, 961, 1032
HspAI GCGC 2 cut(s) 117, 175
Ksp22I TGATCA 1 cut(s) 478
Kzo9I GATC 7 cut(s) 133, 261, 360, 410, 478, 497, 539
Lsp1109I GCAGC 6 cut(s) 41, 184, 249, 354, 357, 583
LweI GCATC 1 cut(s) 1114
MaeI CTAG 1 cut(s) 609
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 1 cut(s) 613
MalI GATC 7 cut(s) 135, 263, 362, 412, 480, 499, 541
MbiI CCGCTC 1 cut(s) 654
MboI GATC 7 cut(s) 133, 261, 360, 410, 478, 497, 539
MboII GAAGA 9 cut(s) 54, 93, 415, 526, 705, 784, 791, 1150, 1160
MhlI GDGCHC 4 cut(s) 333, 532, 580, 587
MluCI AATT 3 cut(s) 94, 373, 1113
MluI ACGCGT 1 cut(s) 811
MlyI GAGTC 1 cut(s) 258
MmeI TCCRAC 2 cut(s) 183, 1067
MseI TTAA 2 cut(s) 1154, 1168
MspA1I CMGCKG 1 cut(s) 148
MspI CCGG 6 cut(s) 334, 573, 580, 624, 642, 899
MspR9I CCNGG 2 cut(s) 580, 643
Mva1269I GAATGC 2 cut(s) 119, 992
MvnI CGCG 3 cut(s) 36, 813, 884
MwoI GCNNNNNNNGC 3 cut(s) 26, 162, 174
NciI CCSGG 2 cut(s) 580, 643
NdeII GATC 7 cut(s) 133, 261, 360, 410, 478, 497, 539
NlaIII CATG 5 cut(s) 52, 487, 847, 961, 1032
NlaIV GGNNCC 5 cut(s) 89, 282, 529, 530, 577
NmeAIII GCCGAG 1 cut(s) 771
NruI TCGCGA 1 cut(s) 36
NsbI TGCGCA 1 cut(s) 118
PcsI WCGNNNNNNNCGW 1 cut(s) 406
PctI GAATGC 2 cut(s) 119, 992
PfeI GAWTC 3 cut(s) 638, 851, 890
PfoI TCCNGGA 1 cut(s) 641
PkrI GCNGC 9 cut(s) 31, 167, 174, 239, 344, 347, 554, 598, 886
Ple19I CGATCG 1 cut(s) 413
PleI GAGTC 1 cut(s) 257
PpsI GAGTC 1 cut(s) 257
Psp124BI GAGCTC 1 cut(s) 587
PspFI CCCAGC 2 cut(s) 296, 1122
PspN4I GGNNCC 5 cut(s) 89, 282, 529, 530, 577
PspOMI GGGCCC 1 cut(s) 528
PspPI GGNCC 2 cut(s) 528, 529
PvuI CGATCG 1 cut(s) 413
PvuII CAGCTG 1 cut(s) 148
RruI TCGCGA 1 cut(s) 36
RsaI GTAC 1 cut(s) 56
RsaNI GTAC 1 cut(s) 55
SacI GAGCTC 1 cut(s) 587
SaqAI TTAA 2 cut(s) 1154, 1168
SatI GCNGC 9 cut(s) 30, 166, 173, 238, 343, 346, 553, 597, 885
Sau3AI GATC 7 cut(s) 133, 261, 360, 410, 478, 497, 539
Sau96I GGNCC 2 cut(s) 528, 529
ScaI AGTACT 1 cut(s) 56
SchI GAGTC 1 cut(s) 258
ScrFI CCNGG 2 cut(s) 580, 643
SduI GDGCHC 4 cut(s) 333, 532, 580, 587
SfaNI GCATC 1 cut(s) 1114
SfcI CTRYAG 1 cut(s) 21
Sse9I AATT 3 cut(s) 94, 373, 1113
SsiI CCGC 9 cut(s) 165, 198, 214, 525, 552, 654, 756, 884, 887
SspMI CTAG 1 cut(s) 609
SstI GAGCTC 1 cut(s) 587
StyD4I CCNGG 2 cut(s) 578, 641
TaaI ACNGT 4 cut(s) 226, 946, 1022, 1042
TaiI ACGT 1 cut(s) 324
TaqI TCGA 8 cut(s) 45, 82, 264, 357, 494, 854, 864, 876
TasI AATT 3 cut(s) 94, 373, 1113
TatI WGTACW 1 cut(s) 54
TauI GCSGC 3 cut(s) 168, 555, 887
TfiI GAWTC 3 cut(s) 638, 851, 890
Tru1I TTAA 2 cut(s) 1154, 1168
Tru9I TTAA 2 cut(s) 1154, 1168
TscAI CASTG 3 cut(s) 229, 805, 1047
TseI GCWGC 6 cut(s) 29, 172, 237, 342, 345, 596
TspDTI ATGAA 5 cut(s) 17, 864, 999, 1017, 1161
TspRI CASTG 3 cut(s) 229, 805, 1047
XcmI CCANNNNNNNNNTGG 1 cut(s) 1119
XspI CTAG 1 cut(s) 609
ZrmI AGTACT 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.