MD16G1057700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014221
Physical Location & Seq
Forward (+)
4124909 .. 4126096
1188 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1057700.v1.1.491

Sequence Viewer

Length: 1188 bp
ATGAAAGAGAGAGGCAAAGCTGTGGAAACAGCTCACGACAATATCGACATGGAGTACTACTCTTCGTCCGAAATGCCGTGTCGAAGACACCCAAATTCGTCTTCCGTCGGGATATGCGCATTCTGTCTAAAAGATCGTTTACTCCAGCTGGTCTGCTCCGACTGCGGCGAGCAGCGCCTCTCTTCCTGCTCCTGCTCCGCCGTCTCGTCAAACCGCAACTCCACCGTGGAGGTCGGCAGCGTCGGCAGAGTCTCGTTTCTGATCGAGAACGAAAAGCACGAGTCTTTACAAAAACCCAACAAAACCCAAGAGAAAGAAGACGTCGTGGTGCTCCGGAGAAGCAGCAGCAGCTGCGTAGAGATCAAGAAAAGTAATTTTTGGAGATTTGGGAGGTTTTTTCGGAAGAAGAGATCGGAAAAAGATGGTGGGAATACGAGTGTTGGTGGGTTTGATGAGAAAAGTGAGATGTGGCTGGTGGATCATATGGGGGTGTCGAGATCAAGGTCTCTCTGCAGTTTCAGAGGTGGGGCACTCTTAGGATCGGAGGACGGCGGAGAACATTTGATGGTTTCCGCCGCCAGGAGCTCCATTTCTGCTGCCAGAAGCTCCAGCGTTACTACTGCCGGAATGGTTCTGGATTCTGGAAGAAAGAGTGGATTTAGTGAGGGGAGAAAGAGTGGATTCAGTGAGGGGAGAAAGAGTGGATTCAGTGAGGGAAGAAAGAGTGGATTCAGTGAAGGGAGAAGGAGCGGATTTAGTGAAGCGGAGCCGAGAAAAAGCGGTTTCGATGGTGAAAAGAAAGATGAAGTTGTAGGGTTTACCGGGGCAACGAAACGCGTTTTTTCGCTGAAGGAGAGTGATTTCGGCATGGATGAATCGGGGTTTATCGATTTGAAGCTCGATTACGCGGCGGATTCGAAGCCAGAGTTTTCTGGCATGAGGATGAGGAGCATTGCAGATGGAGAGACTGTTTTTGGGAGCATGAGAAGGAGTGAGTTTGTGGGAGGTGAATGTGATGTGGGATCATTTGGTATTTTCAGACATGGTGGTTCTTGTAGATTGACAGTGGATGAGAGGGGGATAAAGAGGGGGAGGAAAAGTTTGAAGGGATGGAGGTGGATTTTCAGGCATCATCCAAGCTGGGGAAGTAGTAGGAAGAAAGATGAAGATTTAATGTTCAAAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

396

Amino Acids

43.67

Weight (kDa)

9.24

Isoelectric Point (pI)

54.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014221)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25590
fragaria_vesca FvH4_4g28580
malus_domestica MD13G1052200.v1.1 MD16G1057700.v1.1
prunus_persica Prupe.1G299900_v2.0.a1
pyrus_communis pycom13g04650 pycom16g05050
rosa_chinensis RchiOBHm_Chr4g0436671
rosa_laevigata RLG00000006505
rosa_multiflora Rmu_sc0003130.1_g000019
rosa_roxburghii Rroxscaffold_5G00377750
rosa_rugosa Rorug04G0295100
rosa_samantha Rh4AG349600 Rh4BG358300 Rh4CG372700 Rh4DG352500
rosa_wichuraiana Rw4G030580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 324
Acc16I TGCGCA 1 cut(s) 118
AccBSI CCGCTC 1 cut(s) 750
AccII CGCG 2 cut(s) 837, 908
AccIII TCCGGA 1 cut(s) 333
AclWI GGATC 3 cut(s) 486, 547, 1030
AcsI RAATTY 1 cut(s) 94
AcuI CTGAAG 1 cut(s) 869
AcyI GRCGYC 1 cut(s) 321
AfaI GTAC 1 cut(s) 56
AfiI CCNNNNNNNGG 2 cut(s) 579, 1142
AflIII ACRYGT 1 cut(s) 835
AgsI TTSAA 3 cut(s) 895, 1105, 1180
AjnI CCWGG 1 cut(s) 578
AjuI GAANNNNNNNTTGG 2 cut(s) 85, 117
AluBI AGCT 8 cut(s) 20, 32, 148, 351, 585, 606, 898, 1140
AluI AGCT 8 cut(s) 20, 32, 148, 351, 585, 606, 898, 1140
Alw21I GWGCWC 2 cut(s) 333, 587
Alw26I GTCTC 4 cut(s) 208, 256, 510, 959
AlwI GGATC 3 cut(s) 486, 547, 1030
AlwNI CAGNNNCTG 1 cut(s) 351
Aor13HI TCCGGA 1 cut(s) 333
ApeKI GCWGC 7 cut(s) 172, 237, 342, 345, 348, 351, 596
ApoI RAATTY 1 cut(s) 94
AspLEI GCGC 2 cut(s) 119, 177
AsuC2I CCSGG 1 cut(s) 823
AsuHPI GGTGA 2 cut(s) 803, 1019
AsuII TTCGAA 1 cut(s) 917
BaeGI GKGCMC 1 cut(s) 532
BanII GRGCYC 1 cut(s) 587
BarI GAAGNNNNNNTAC 2 cut(s) 887, 919
BauI CACGAG 1 cut(s) 278
BbsI GAAGAC 3 cut(s) 91, 93, 324
Bbv12I GWGCWC 2 cut(s) 333, 587
BbvI GCAGC 7 cut(s) 184, 249, 338, 354, 357, 360, 583
BccI CCATC 5 cut(s) 416, 559, 782, 953, 1104
BceAI ACGGC 3 cut(s) 61, 185, 565
BciT130I CCWGG 1 cut(s) 580
BcnI CCSGG 1 cut(s) 823
BcoDI GTCTC 4 cut(s) 208, 256, 510, 959
BfmI CTRYAG 1 cut(s) 511
BfoI RGCGCY 1 cut(s) 178
BmcAI AGTACT 1 cut(s) 56
Bme1390I CCNGG 2 cut(s) 580, 823
BmiI GGNNCC 1 cut(s) 768
BmrFI CCNGG 2 cut(s) 580, 823
BmsI GCATC 1 cut(s) 1138
BpiI GAAGAC 3 cut(s) 91, 93, 324
BplI GAGNNNNNCTC 2 cut(s) 44, 76
BpmI CTGGAG 2 cut(s) 128, 592
Bpu14I TTCGAA 1 cut(s) 917
BpuMI CCSGG 1 cut(s) 823
Bsa29I ATCGAT 1 cut(s) 888
BsaHI GRCGYC 1 cut(s) 321
BsaI GGTCTC 1 cut(s) 510
BsaJI CCNNGG 2 cut(s) 225, 822
BsaWI WCCGGW 1 cut(s) 333
Bsc4I CCNNNNNNNGG 2 cut(s) 579, 1142
Bse3DI GCAATG 1 cut(s) 951
BseAI TCCGGA 1 cut(s) 333
BseBI CCWGG 1 cut(s) 580
BseCI ATCGAT 1 cut(s) 888
BseDI CCNNGG 2 cut(s) 225, 822
BseGI GGATG 5 cut(s) 877, 948, 1075, 1115, 1132
BseLI CCNNNNNNNGG 2 cut(s) 579, 1142
BseMI GCAATG 1 cut(s) 951
BseRI GAGGAG 1 cut(s) 961
BseSI GKGCMC 1 cut(s) 532
BseXI GCAGC 7 cut(s) 184, 249, 338, 354, 357, 360, 583
BseYI CCCAGC 1 cut(s) 1140
Bsh1236I CGCG 2 cut(s) 837, 908
BshVI ATCGAT 1 cut(s) 888
BsiHKAI GWGCWC 2 cut(s) 333, 587
BsiSI CCGG 3 cut(s) 334, 624, 822
BslI CCNNNNNNNGG 2 cut(s) 579, 1142
BsmAI GTCTC 4 cut(s) 208, 256, 510, 959
BsmBI CGTCTC 1 cut(s) 208
BsmI GAATGC 1 cut(s) 119
Bso31I GGTCTC 1 cut(s) 510
Bsp119I TTCGAA 1 cut(s) 917
Bsp1286I GDGCHC 3 cut(s) 333, 532, 587
Bsp13I TCCGGA 1 cut(s) 333
Bsp143I GATC 8 cut(s) 133, 261, 360, 410, 478, 497, 539, 1022
BspDI ATCGAT 1 cut(s) 888
BspEI TCCGGA 1 cut(s) 333
BspFNI CGCG 2 cut(s) 837, 908
BspLI GGNNCC 1 cut(s) 768
BspMAI CTGCAG 1 cut(s) 515
BspPI GGATC 3 cut(s) 486, 547, 1030
BspT104I TTCGAA 1 cut(s) 917
BspTNI GGTCTC 1 cut(s) 510
BsrBI CCGCTC 1 cut(s) 750
BsrDI GCAATG 1 cut(s) 951
BssECI CCNNGG 2 cut(s) 225, 822
BssMI GATC 8 cut(s) 133, 261, 360, 410, 478, 497, 539, 1022
BssNI GRCGYC 1 cut(s) 321
BssSI CACGAG 1 cut(s) 278
Bst2BI CACGAG 1 cut(s) 278
Bst2UI CCWGG 1 cut(s) 580
Bst4CI ACNGT 3 cut(s) 226, 970, 1066
Bst6I CTCTTC 3 cut(s) 67, 187, 401
BstACI GRCGYC 1 cut(s) 321
BstAPI GCANNNNNTGC 1 cut(s) 351
BstBI TTCGAA 1 cut(s) 917
BstC8I GCNNGC 1 cut(s) 170
BstDEI CTNAG 1 cut(s) 535
BstDSI CCRYGG 1 cut(s) 225
BstF5I GGATG 5 cut(s) 877, 948, 1075, 1115, 1132
BstFNI CGCG 2 cut(s) 837, 908
BstH2I RGCGCY 1 cut(s) 178
BstHHI GCGC 2 cut(s) 119, 177
BstKTI GATC 8 cut(s) 136, 264, 363, 413, 481, 500, 542, 1025
BstMAI GTCTC 4 cut(s) 208, 256, 510, 959
BstMBI GATC 8 cut(s) 133, 261, 360, 410, 478, 497, 539, 1022
BstMWI GCNNNNNNNGC 5 cut(s) 162, 174, 243, 348, 351
BstNI CCWGG 1 cut(s) 580
BstSCI CCNGG 2 cut(s) 578, 821
BstSFI CTRYAG 1 cut(s) 511
BstSLI GKGCMC 1 cut(s) 532
BstUI CGCG 2 cut(s) 837, 908
BstV1I GCAGC 7 cut(s) 184, 249, 338, 354, 357, 360, 583
BstV2I GAAGAC 3 cut(s) 91, 93, 324
Bsu15I ATCGAT 1 cut(s) 888
BsuTUI ATCGAT 1 cut(s) 888
BtgI CCRYGG 1 cut(s) 225
BtsCI GGATG 5 cut(s) 877, 948, 1075, 1115, 1132
BtsIMutI CAGTG 4 cut(s) 691, 715, 739, 1071
Cac8I GCNNGC 1 cut(s) 170
CaiI CAGNNNCTG 1 cut(s) 351
CfoI GCGC 2 cut(s) 119, 177
ClaI ATCGAT 1 cut(s) 888
CseI GACGC 1 cut(s) 229
Csp6I GTAC 1 cut(s) 55
CviAII CATG 5 cut(s) 49, 868, 937, 982, 1043
CviQI GTAC 1 cut(s) 55
DdeI CTNAG 1 cut(s) 535
DpnI GATC 8 cut(s) 135, 263, 362, 412, 480, 499, 541, 1024
DpnII GATC 8 cut(s) 133, 261, 360, 410, 478, 497, 539, 1022
Eam1104I CTCTTC 3 cut(s) 67, 187, 401
EarI CTCTTC 3 cut(s) 67, 187, 401
EciI GGCGGA 4 cut(s) 187, 562, 567, 926
Ecl136II GAGCTC 1 cut(s) 585
Eco24I GRGCYC 1 cut(s) 587
Eco31I GGTCTC 1 cut(s) 510
Eco53kI GAGCTC 1 cut(s) 585
Eco57I CTGAAG 1 cut(s) 869
EcoICRI GAGCTC 1 cut(s) 585
EcoRII CCWGG 1 cut(s) 578
EcoT38I GRGCYC 1 cut(s) 587
Esp3I CGTCTC 1 cut(s) 208
FaeI CATG 5 cut(s) 52, 871, 940, 985, 1046
FaiI YATR 8 cut(s) 50, 115, 483, 485, 869, 938, 983, 1044
FatI CATG 5 cut(s) 48, 867, 936, 981, 1042
FauNDI CATATG 1 cut(s) 483
FokI GGATG 5 cut(s) 884, 955, 1082, 1119, 1122
FriOI GRGCYC 1 cut(s) 587
FspAI RTGCGCAY 1 cut(s) 118
FspI TGCGCA 1 cut(s) 118
GlaI GCGC 2 cut(s) 118, 176
GsaI CCCAGC 1 cut(s) 1144
GsuI CTGGAG 2 cut(s) 128, 592
HaeII RGCGCY 1 cut(s) 178
HapII CCGG 3 cut(s) 334, 624, 822
HgaI GACGC 1 cut(s) 229
HhaI GCGC 2 cut(s) 119, 177
Hin1I GRCGYC 1 cut(s) 321
Hin1II CATG 5 cut(s) 52, 871, 940, 985, 1046
Hin6I GCGC 2 cut(s) 117, 175
HinP1I GCGC 2 cut(s) 117, 175
HinfI GANTC 8 cut(s) 249, 281, 638, 681, 705, 729, 875, 914
HpaII CCGG 3 cut(s) 334, 624, 822
HphI GGTGA 2 cut(s) 803, 1019
Hpy166II GTNNAC 2 cut(s) 140, 819
Hpy188I TCNGA 8 cut(s) 70, 160, 261, 402, 415, 521, 544, 1040
Hpy188III TCNNGA 8 cut(s) 35, 109, 265, 334, 364, 495, 635, 642
Hpy8I GTNNAC 2 cut(s) 140, 819
Hpy99I CGWCG 3 cut(s) 110, 245, 326
HpyAV CCTTC 5 cut(s) 731, 738, 844, 981, 1099
HpyCH4III ACNGT 3 cut(s) 226, 970, 1066
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 2 cut(s) 513, 956
HpyF10VI GCNNNNNNNGC 5 cut(s) 162, 174, 243, 348, 351
HpyF3I CTNAG 1 cut(s) 535
HpySE526I ACGT 1 cut(s) 321
Hsp92I GRCGYC 1 cut(s) 321
Hsp92II CATG 5 cut(s) 52, 871, 940, 985, 1046
HspAI GCGC 2 cut(s) 117, 175
Kpn2I TCCGGA 1 cut(s) 333
Kzo9I GATC 8 cut(s) 133, 261, 360, 410, 478, 497, 539, 1022
Lsp1109I GCAGC 7 cut(s) 184, 249, 338, 354, 357, 360, 583
LweI GCATC 1 cut(s) 1138
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 1 cut(s) 613
MalI GATC 8 cut(s) 135, 263, 362, 412, 480, 499, 541, 1024
MbiI CCGCTC 1 cut(s) 750
MboI GATC 8 cut(s) 133, 261, 360, 410, 478, 497, 539, 1022
MhlI GDGCHC 3 cut(s) 333, 532, 587
MluCI AATT 2 cut(s) 94, 373
MluI ACGCGT 1 cut(s) 835
MlyI GAGTC 2 cut(s) 258, 290
MmeI TCCRAC 1 cut(s) 183
MroI TCCGGA 1 cut(s) 333
MseI TTAA 2 cut(s) 1172, 1186
MslI CAYNNNNRTG 1 cut(s) 941
MspA1I CMGCKG 2 cut(s) 148, 351
MspI CCGG 3 cut(s) 334, 624, 822
MspR9I CCNGG 2 cut(s) 580, 823
Mva1269I GAATGC 1 cut(s) 119
MvaI CCWGG 1 cut(s) 580
MvnI CGCG 2 cut(s) 837, 908
MwoI GCNNNNNNNGC 5 cut(s) 162, 174, 243, 348, 351
NciI CCSGG 1 cut(s) 823
NdeI CATATG 1 cut(s) 483
NdeII GATC 8 cut(s) 133, 261, 360, 410, 478, 497, 539, 1022
NlaIII CATG 5 cut(s) 52, 871, 940, 985, 1046
NlaIV GGNNCC 1 cut(s) 768
NmeAIII GCCGAG 1 cut(s) 795
NsbI TGCGCA 1 cut(s) 118
NspV TTCGAA 1 cut(s) 917
PcsI WCGNNNNNNNCGW 2 cut(s) 42, 276
PctI GAATGC 1 cut(s) 119
PfeI GAWTC 6 cut(s) 638, 681, 705, 729, 875, 914
PleI GAGTC 2 cut(s) 257, 289
PpsI GAGTC 2 cut(s) 257, 289
Psp124BI GAGCTC 1 cut(s) 587
Psp6I CCWGG 1 cut(s) 578
PspFI CCCAGC 1 cut(s) 1140
PspGI CCWGG 1 cut(s) 578
PspN4I GGNNCC 1 cut(s) 768
PstI CTGCAG 1 cut(s) 515
PstNI CAGNNNCTG 1 cut(s) 351
PvuII CAGCTG 2 cut(s) 148, 351
RsaI GTAC 1 cut(s) 56
RsaNI GTAC 1 cut(s) 55
RseI CAYNNNNRTG 1 cut(s) 941
SacI GAGCTC 1 cut(s) 587
SaqAI TTAA 2 cut(s) 1172, 1186
Sau3AI GATC 8 cut(s) 133, 261, 360, 410, 478, 497, 539, 1022
ScaI AGTACT 1 cut(s) 56
SchI GAGTC 2 cut(s) 258, 290
ScrFI CCNGG 2 cut(s) 580, 823
SduI GDGCHC 3 cut(s) 333, 532, 587
SfaNI GCATC 1 cut(s) 1138
SfcI CTRYAG 1 cut(s) 511
SfuI TTCGAA 1 cut(s) 917
SmiMI CAYNNNNRTG 1 cut(s) 941
Sse9I AATT 2 cut(s) 94, 373
SstI GAGCTC 1 cut(s) 587
StyD4I CCNGG 2 cut(s) 578, 821
TaaI ACNGT 3 cut(s) 226, 970, 1066
TaiI ACGT 1 cut(s) 324
TaqI TCGA 8 cut(s) 45, 82, 264, 494, 786, 888, 900, 917
TasI AATT 2 cut(s) 94, 373
TatI WGTACW 1 cut(s) 54
TauI GCSGC 3 cut(s) 168, 578, 911
TfiI GAWTC 6 cut(s) 638, 681, 705, 729, 875, 914
Tru1I TTAA 2 cut(s) 1172, 1186
Tru9I TTAA 2 cut(s) 1172, 1186
TscAI CASTG 4 cut(s) 691, 715, 739, 1071
TseI GCWGC 7 cut(s) 172, 237, 342, 345, 348, 351, 596
TspDTI ATGAA 4 cut(s) 17, 819, 888, 1179
TspGWI ACGGA 1 cut(s) 94
TspRI CASTG 4 cut(s) 691, 715, 739, 1071
XapI RAATTY 1 cut(s) 94
ZraI GACGTC 1 cut(s) 322
ZrmI AGTACT 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.