Prupe.1G299900_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0014221
Physical Location & Seq
Forward (+)
29489017 .. 29491057
2041 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G299900.1

Sequence Viewer

Length: 1257 bp
ATGAAAGACAGAGGCAAAGCTGTGGAAGCACATAACAACATTGACATGGAGTACTTTTCTTCATCTGAAATGCCATGTAGAAAACACCCCAATTCATCATCAGTTGGGATATGCGCTTACTGTCTTAAAGAACGTTTGGTCAAGCTCGTCTGCTCAGATTGTGGCGAGCAGCGTCTCTCTTCCTGCTCTTGCTCTGAGATCTCATCAAACCGAAACTCATGCACTGTGGAGGTAGGCAGCGTTGGCAGAGTCTCATTTCTGATTGAGAATGAAAAGCATGAGGCTTTACAATCAAATTCCAAGGCCAACAAGACCCAAGAGAAAGAGGATGTTGTGGTGCTCAGGAGAAGCAGCAGTAGCTGCGTTGAGATTAAAAGAAGTGGGTTTTGGAGATTTGGGCGGTTGTTTAGGAAGAAAAGATCAGACAAAGATTGTGGGAACCAAAGTGTTGTTGGGTTTGATGAGAAAAGCGAGATGTGGCTCGTTGATCATATGGGCGTGTCAAGGTCCAGGTCTCTCTGCAGTTTCAGGGGCGGTGGCTTTTTTGGATCAGAGGATGGTGGAGAACATTTAATGGTTTCTGGGGCCAGGAGCTCAATTTCAGCTGCAAGAAGTTCCAGTGTCACTACTGCTGGTATGGTTTTAGATTCTGGAAGAAAAAGTGGGTTCAGTGAAGGAAGAAAGAGCGGATTCAGTGAAGGAAGAAAGAGCGGATTCAGTGAAGGAAGAAAGAGTGGGTTCAGTGAAGCAGAGCCAAGAAAAAGTGGTTTTGATGGTGAAAAGAAAGATGAGGTTTTGGCTTCTGAATTGGGTACTGATTTTAAGGGTGCCAAGAAAAATGGCCTTATGGAGGCTGCTGATAAGGGTTTCAGTGGGGCAACAAGAAGAGTTTTCTCACTCAAGGAGAGTGATTTTAGTGGCATGGATGAATCAGGGTTTATTGACTTGAAGCTTGATTATTCAGCAGAGTCCAAGCCAGAATTCTCTGCAATGAAGATGGGAAATATCACAGATACAGATTCTGTATTTGGAAGCATGAGAGGCAGTGACTTTGTGGCAAATGAATGTGGAGGTCCATTTGGAGGTCTAATTGGAGATGGTATTTTCAGCCATGGAGGTTCTTGTAGAATAACAGTCAATGACAGAGGGATCAAGAGGGGGAGGAAAAGTTCGAAGGGTTGGAAATGGATTTTCAGGCATCATCCAAGCTGGGGAAGTACTAGGAAGAAAGATGAAGATTTAATGTTCAAAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

419

Amino Acids

45.71

Weight (kDa)

9.0

Isoelectric Point (pI)

47.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014221)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25590
fragaria_vesca FvH4_4g28580
malus_domestica MD13G1052200.v1.1 MD16G1057700.v1.1
prunus_persica Prupe.1G299900_v2.0.a1
pyrus_communis pycom13g04650 pycom16g05050
rosa_chinensis RchiOBHm_Chr4g0436671
rosa_laevigata RLG00000006505
rosa_multiflora Rmu_sc0003130.1_g000019
rosa_roxburghii Rroxscaffold_5G00377750
rosa_rugosa Rorug04G0295100
rosa_samantha Rh4AG349600 Rh4BG358300 Rh4CG372700 Rh4DG352500
rosa_wichuraiana Rw4G030580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 827
AccBSI CCGCTC 2 cut(s) 687, 711
AciI CCGC 4 cut(s) 400, 534, 687, 711
AclI AACGTT 1 cut(s) 133
AclWI GGATC 2 cut(s) 556, 1157
AcsI RAATTY 2 cut(s) 295, 980
AfaI GTAC 3 cut(s) 53, 814, 1219
AfiI CCNNNNNNNGG 3 cut(s) 850, 1082, 1211
AgsI TTSAA 2 cut(s) 949, 1249
AjnI CCWGG 2 cut(s) 509, 587
AjuI GAANNNNNNNTTGG 4 cut(s) 370, 402, 965, 997
AloI GAACNNNNNNTCC 2 cut(s) 1153, 1185
AluBI AGCT 7 cut(s) 20, 145, 360, 594, 605, 952, 1209
AluI AGCT 7 cut(s) 20, 145, 360, 594, 605, 952, 1209
Alw21I GWGCWC 2 cut(s) 342, 596
Alw26I GTCTC 3 cut(s) 179, 256, 519
AlwI GGATC 2 cut(s) 556, 1157
AlwNI CAGNNNCTG 2 cut(s) 360, 1022
AoxI GGCC 3 cut(s) 303, 585, 841
ApeKI GCWGC 6 cut(s) 169, 237, 351, 360, 605, 854
ApoI RAATTY 2 cut(s) 295, 980
AspLEI GCGC 1 cut(s) 116
AspS9I GGNCC 3 cut(s) 507, 585, 1073
AsuHPI GGTGA 1 cut(s) 788
AsuII TTCGAA 1 cut(s) 1172
AvaII GGWCC 2 cut(s) 507, 1073
BanI GGYRCC 1 cut(s) 827
BanII GRGCYC 1 cut(s) 596
Bbv12I GWGCWC 2 cut(s) 342, 596
BbvI GCAGC 6 cut(s) 181, 249, 347, 363, 592, 841
BccI CCATC 4 cut(s) 551, 767, 991, 1091
BcgI CGANNNNNNTGC 2 cut(s) 201, 235
BciT130I CCWGG 2 cut(s) 511, 589
BclI TGATCA 1 cut(s) 487
BcoDI GTCTC 3 cut(s) 179, 256, 519
BfaI CTAG 1 cut(s) 1221
BfmI CTRYAG 1 cut(s) 520
BglII AGATCT 1 cut(s) 198
BisI GCNGC 6 cut(s) 170, 238, 352, 361, 606, 855
BlsI GCNGC 6 cut(s) 171, 239, 353, 362, 607, 856
BmcAI AGTACT 2 cut(s) 53, 1219
Bme1390I CCNGG 2 cut(s) 511, 589
Bme18I GGWCC 2 cut(s) 507, 1073
BmgT120I GGNCC 3 cut(s) 507, 585, 1073
BmiI GGNNCC 3 cut(s) 440, 586, 829
BmrFI CCNGG 2 cut(s) 511, 589
BmsI GCATC 1 cut(s) 1207
Bpu10I CCTNAGC 1 cut(s) 341
Bpu14I TTCGAA 1 cut(s) 1172
BpuEI CTTGAG 1 cut(s) 884
BsaI GGTCTC 1 cut(s) 519
BsaJI CCNNGG 2 cut(s) 300, 1111
BsaXI ACNNNNNCTCC 2 cut(s) 1153, 1183
Bsc4I CCNNNNNNNGG 3 cut(s) 850, 1082, 1211
Bse1I ACTGG 1 cut(s) 618
Bse3DI GCAATG 1 cut(s) 996
BseBI CCWGG 2 cut(s) 511, 589
BseDI CCNNGG 2 cut(s) 300, 1111
BseGI GGATG 4 cut(s) 334, 562, 931, 1201
BseLI CCNNNNNNNGG 3 cut(s) 850, 1082, 1211
BseMI GCAATG 1 cut(s) 996
BseMII CTCAG 3 cut(s) 168, 186, 355
BseNI ACTGG 1 cut(s) 618
BseXI GCAGC 6 cut(s) 181, 249, 347, 363, 592, 841
BseYI CCCAGC 1 cut(s) 1209
BshFI GGCC 3 cut(s) 305, 587, 843
BshNI GGYRCC 1 cut(s) 827
BsiHKAI GWGCWC 2 cut(s) 342, 596
BslI CCNNNNNNNGG 3 cut(s) 850, 1082, 1211
BsmAI GTCTC 3 cut(s) 179, 256, 519
BsmBI CGTCTC 1 cut(s) 179
BsnI GGCC 3 cut(s) 305, 587, 843
Bso31I GGTCTC 1 cut(s) 519
Bsp119I TTCGAA 1 cut(s) 1172
Bsp1286I GDGCHC 2 cut(s) 342, 596
Bsp143I GATC 5 cut(s) 198, 419, 487, 548, 1149
Bsp19I CCATGG 1 cut(s) 1111
BspACI CCGC 4 cut(s) 400, 534, 687, 711
BspANI GGCC 3 cut(s) 305, 587, 843
BspCNI CTCAG 3 cut(s) 167, 187, 354
BspLI GGNNCC 3 cut(s) 440, 586, 829
BspMAI CTGCAG 1 cut(s) 524
BspPI GGATC 2 cut(s) 556, 1157
BspT104I TTCGAA 1 cut(s) 1172
BspT107I GGYRCC 1 cut(s) 827
BspTNI GGTCTC 1 cut(s) 519
BsrBI CCGCTC 2 cut(s) 687, 711
BsrDI GCAATG 1 cut(s) 996
BsrI ACTGG 1 cut(s) 618
BssECI CCNNGG 2 cut(s) 300, 1111
BssMI GATC 5 cut(s) 198, 419, 487, 548, 1149
BssT1I CCWWGG 2 cut(s) 300, 1111
Bst2UI CCWGG 2 cut(s) 511, 589
Bst4CI ACNGT 3 cut(s) 122, 226, 1135
Bst6I CTCTTC 2 cut(s) 184, 880
BstAPI GCANNNNNTGC 1 cut(s) 360
BstBI TTCGAA 1 cut(s) 1172
BstC8I GCNNGC 1 cut(s) 167
BstDEI CTNAG 3 cut(s) 154, 195, 341
BstDSI CCRYGG 1 cut(s) 1111
BstENI CCTNNNNNAGG 1 cut(s) 848
BstF5I GGATG 4 cut(s) 334, 562, 931, 1201
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 5 cut(s) 201, 422, 490, 551, 1152
BstMAI GTCTC 3 cut(s) 179, 256, 519
BstMBI GATC 5 cut(s) 198, 419, 487, 548, 1149
BstMWI GCNNNNNNNGC 5 cut(s) 26, 243, 357, 360, 1041
BstNI CCWGG 2 cut(s) 511, 589
BstSCI CCNGG 2 cut(s) 509, 587
BstSFI CTRYAG 1 cut(s) 520
BstV1I GCAGC 6 cut(s) 181, 249, 347, 363, 592, 841
BstX2I RGATCY 1 cut(s) 198
BstYI RGATCY 1 cut(s) 198
BsuRI GGCC 3 cut(s) 305, 587, 843
BtgI CCRYGG 1 cut(s) 1111
BtsCI GGATG 4 cut(s) 334, 562, 931, 1201
BtsI GCAGTG 1 cut(s) 1051
BtsIMutI CAGTG 8 cut(s) 222, 625, 676, 700, 724, 748, 877, 1051
Cac8I GCNNGC 1 cut(s) 167
CaiI CAGNNNCTG 2 cut(s) 360, 1022
CfoI GCGC 1 cut(s) 116
Cfr13I GGNCC 3 cut(s) 507, 585, 1073
CseI GACGC 1 cut(s) 161
Csp6I GTAC 3 cut(s) 52, 813, 1218
CspCI CAANNNNNGTGG 2 cut(s) 415, 450
CviAII CATG 7 cut(s) 46, 75, 219, 278, 922, 1036, 1112
CviQI GTAC 3 cut(s) 52, 813, 1218
DdeI CTNAG 3 cut(s) 154, 195, 341
DpnI GATC 5 cut(s) 200, 421, 489, 550, 1151
DpnII GATC 5 cut(s) 198, 419, 487, 548, 1149
Eam1104I CTCTTC 2 cut(s) 184, 880
EarI CTCTTC 2 cut(s) 184, 880
Ecl136II GAGCTC 1 cut(s) 594
Eco130I CCWWGG 2 cut(s) 300, 1111
Eco24I GRGCYC 1 cut(s) 596
Eco31I GGTCTC 1 cut(s) 519
Eco47I GGWCC 2 cut(s) 507, 1073
Eco53kI GAGCTC 1 cut(s) 594
EcoICRI GAGCTC 1 cut(s) 594
EcoNI CCTNNNNNAGG 1 cut(s) 848
EcoRI GAATTC 1 cut(s) 980
EcoRII CCWGG 2 cut(s) 509, 587
EcoT14I CCWWGG 2 cut(s) 300, 1111
EcoT38I GRGCYC 1 cut(s) 596
ErhI CCWWGG 2 cut(s) 300, 1111
Esp3I CGTCTC 1 cut(s) 179
FaeI CATG 7 cut(s) 49, 78, 222, 281, 925, 1039, 1115
FatI CATG 7 cut(s) 45, 74, 218, 277, 921, 1035, 1111
FauNDI CATATG 1 cut(s) 492
FbaI TGATCA 1 cut(s) 487
Fnu4HI GCNGC 6 cut(s) 170, 238, 352, 361, 606, 855
FokI GGATG 4 cut(s) 341, 569, 938, 1188
FriOI GRGCYC 1 cut(s) 596
Fsp4HI GCNGC 6 cut(s) 170, 238, 352, 361, 606, 855
FspBI CTAG 1 cut(s) 1221
GlaI GCGC 1 cut(s) 115
GluI GCNGC 6 cut(s) 170, 238, 352, 361, 606, 855
GsaI CCCAGC 1 cut(s) 1213
HaeIII GGCC 3 cut(s) 305, 587, 843
HgaI GACGC 1 cut(s) 161
HhaI GCGC 1 cut(s) 116
Hin1II CATG 7 cut(s) 49, 78, 222, 281, 925, 1039, 1115
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HindIII AAGCTT 1 cut(s) 950
HinfI GANTC 7 cut(s) 249, 647, 690, 714, 929, 968, 1019
HphI GGTGA 1 cut(s) 788
Hpy188I TCNGA 7 cut(s) 67, 157, 196, 261, 424, 553, 805
Hpy188III TCNNGA 3 cut(s) 343, 651, 1153
HpyAV CCTTC 4 cut(s) 668, 692, 716, 1168
HpyCH4III ACNGT 3 cut(s) 122, 226, 1135
HpyCH4IV ACGT 1 cut(s) 133
HpyCH4V TGCA 4 cut(s) 222, 522, 608, 989
HpyF10VI GCNNNNNNNGC 5 cut(s) 26, 243, 357, 360, 1041
HpyF3I CTNAG 3 cut(s) 154, 195, 341
HpySE526I ACGT 1 cut(s) 133
Hsp92II CATG 7 cut(s) 49, 78, 222, 281, 925, 1039, 1115
HspAI GCGC 1 cut(s) 114
Ksp22I TGATCA 1 cut(s) 487
Kzo9I GATC 5 cut(s) 198, 419, 487, 548, 1149
LmnI GCTCC 1 cut(s) 591
Lsp1109I GCAGC 6 cut(s) 181, 249, 347, 363, 592, 841
LweI GCATC 1 cut(s) 1207
MaeI CTAG 1 cut(s) 1221
MaeII ACGT 1 cut(s) 133
MaeIII GTNAC 2 cut(s) 622, 1046
MalI GATC 5 cut(s) 200, 421, 489, 550, 1151
MbiI CCGCTC 2 cut(s) 687, 711
MboI GATC 5 cut(s) 198, 419, 487, 548, 1149
MflI RGATCY 1 cut(s) 198
MhlI GDGCHC 2 cut(s) 342, 596
MluCI AATT 6 cut(s) 91, 295, 597, 806, 980, 1089
MlyI GAGTC 2 cut(s) 258, 977
MmeI TCCRAC 1 cut(s) 1160
MseI TTAA 5 cut(s) 126, 372, 572, 822, 1241
MslI CAYNNNNRTG 1 cut(s) 44
MspA1I CMGCKG 1 cut(s) 605
MspR9I CCNGG 2 cut(s) 511, 589
MvaI CCWGG 2 cut(s) 511, 589
MwoI GCNNNNNNNGC 5 cut(s) 26, 243, 357, 360, 1041
NcoI CCATGG 1 cut(s) 1111
NdeI CATATG 1 cut(s) 492
NdeII GATC 5 cut(s) 198, 419, 487, 548, 1149
NlaIII CATG 7 cut(s) 49, 78, 222, 281, 925, 1039, 1115
NlaIV GGNNCC 3 cut(s) 440, 586, 829
NmuCI GTSAC 2 cut(s) 622, 1046
NspV TTCGAA 1 cut(s) 1172
PfeI GAWTC 5 cut(s) 647, 690, 714, 929, 1019
PkrI GCNGC 6 cut(s) 171, 239, 353, 362, 607, 856
PleI GAGTC 2 cut(s) 257, 976
PpsI GAGTC 2 cut(s) 257, 976
Psp124BI GAGCTC 1 cut(s) 596
Psp1406I AACGTT 1 cut(s) 133
Psp6I CCWGG 2 cut(s) 509, 587
PspFI CCCAGC 1 cut(s) 1209
PspGI CCWGG 2 cut(s) 509, 587
PspN4I GGNNCC 3 cut(s) 440, 586, 829
PspPI GGNCC 3 cut(s) 507, 585, 1073
PstI CTGCAG 1 cut(s) 524
PstNI CAGNNNCTG 2 cut(s) 360, 1022
PsuI RGATCY 1 cut(s) 198
PvuII CAGCTG 1 cut(s) 605
RsaI GTAC 3 cut(s) 53, 814, 1219
RsaNI GTAC 3 cut(s) 52, 813, 1218
RseI CAYNNNNRTG 1 cut(s) 44
SacI GAGCTC 1 cut(s) 596
SaqAI TTAA 5 cut(s) 126, 372, 572, 822, 1241
SatI GCNGC 6 cut(s) 170, 238, 352, 361, 606, 855
Sau3AI GATC 5 cut(s) 198, 419, 487, 548, 1149
Sau96I GGNCC 3 cut(s) 507, 585, 1073
ScaI AGTACT 2 cut(s) 53, 1219
SchI GAGTC 2 cut(s) 258, 977
ScrFI CCNGG 2 cut(s) 511, 589
SduI GDGCHC 2 cut(s) 342, 596
SfaNI GCATC 1 cut(s) 1207
SfcI CTRYAG 1 cut(s) 520
SfuI TTCGAA 1 cut(s) 1172
SinI GGWCC 2 cut(s) 507, 1073
SmiMI CAYNNNNRTG 1 cut(s) 44
SmlI CTYRAG 1 cut(s) 899
SmoI CTYRAG 1 cut(s) 899
Sse9I AATT 6 cut(s) 91, 295, 597, 806, 980, 1089
SsiI CCGC 4 cut(s) 400, 534, 687, 711
SspMI CTAG 1 cut(s) 1221
SstI GAGCTC 1 cut(s) 596
StyD4I CCNGG 2 cut(s) 509, 587
StyI CCWWGG 2 cut(s) 300, 1111
TaaI ACNGT 3 cut(s) 122, 226, 1135
TaiI ACGT 1 cut(s) 136
TaqI TCGA 1 cut(s) 1172
TasI AATT 6 cut(s) 91, 295, 597, 806, 980, 1089
TatI WGTACW 2 cut(s) 51, 1217
TfiI GAWTC 5 cut(s) 647, 690, 714, 929, 1019
Tru1I TTAA 5 cut(s) 126, 372, 572, 822, 1241
Tru9I TTAA 5 cut(s) 126, 372, 572, 822, 1241
TscAI CASTG 8 cut(s) 229, 625, 676, 700, 724, 748, 877, 1051
TseFI GTSAC 2 cut(s) 622, 1046
TseI GCWGC 6 cut(s) 169, 237, 351, 360, 605, 854
Tsp45I GTSAC 2 cut(s) 622, 1046
TspDTI ATGAA 8 cut(s) 17, 51, 84, 285, 942, 1007, 1077, 1248
TspRI CASTG 8 cut(s) 229, 625, 676, 700, 724, 748, 877, 1051
VpaK11BI GGWCC 2 cut(s) 507, 1073
XagI CCTNNNNNAGG 1 cut(s) 848
XapI RAATTY 2 cut(s) 295, 980
XcmI CCANNNNNNNNNTGG 1 cut(s) 449
XspI CTAG 1 cut(s) 1221
ZrmI AGTACT 2 cut(s) 53, 1219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.