AT3G27060

ribonucleoside-diphosphate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0013930
Physical Location & Seq
Reverse (-)
9979841 .. 9981620
1780 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G27060.1

Sequence Viewer

Length: 999 bp
ATGCCTTCAATGCCAGAAGAGCCGCTCCTAACACCAACCCCAGACAGATTCTGTATGTTCCCAATTCACTACCCACAGATCTGGGAAATGTACAAAAAAGCCGAAGCATCATTCTGGACCGCCGAAGAAGTAGATCTATCACAAGACAACCGCGACTGGGAAAACAGTCTCAACGACGGTGAACGTCACTTCATCAAACACGTCCTCGCTTTCTTCGCTGCATCTGACGGAATCGTACTAGAGAATCTCGCTTCTCGCTTCATGTCCGATGTTCAAGTCTCTGAGGCGCGTGCTTTCTACGGTTTCCAGATCGCGATTGAAAACATCCACTCGGAGATGTATAGTCTCCTTCTCGATACTTATATCAAAGATAACAAAGAGAGAGATCATCTCTTTCGTGCCATTGAAACAATCCCTTGCGTCGCCAAGAAAGCTCAATGGGCTATGAAATGGATCGACGGTTCTCAGACTTTCGCCGAACGAATCATCGCTTTCGCTTGCGTCGAAGGTATCTTCTTCTCCGGAAGCTTTTGTTCAATCTTCTGGCTGAAGAAACGAGGACTCATGCCTGGATTAACATTCTCAAACGAATTGATCTCACGTGACGAAGGTTTACACTGTGATTTCGCTTGTCTACTCTACACGCTACTCAAAACAAAGCTTAGCGAAGAACGCGTGAAATCAATCGTCTGCGACGCGGTCGAAATCGAGAGAGAGTTTGTGTGCGACGCGCTTCCGTGCGCGTTGGTTGGGATGAACCGTGATTTGATGAGTCAGTATATTGAGTTTGTTGCGGATAGGCTTTTGGGTGCGCTTGGGTACGGGAAGGTGTACGGTGTTACTAATCCGTTCGATTGGATGGAACTTATTTCACTTCAAGGGAAAACGAATTTCTTCGAGAAACGTGTTGGTGATTACCAAAAGGCTTCCGTTATGTCTAGCGTTAATGGTAACGGCGCGTTTGATAACCATGTCTTCTCTCTCGACGAAGATTTTTAA

Protein Analysis

332

Amino Acids

38.03

Weight (kDa)

4.86

Isoelectric Point (pI)

42.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuc_red_sm PF00268 16 - 283 2.4e-118 Ribonucleotide reductase, small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013930)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27060
fragaria_vesca FvH4_6g53560
malus_domestica MD00G1139000.v1.1 MD09G1010500.v1.1
prunus_persica Prupe.3G306600_v2.0.a1
pyrus_communis pycom111g00840 pycom17g00920
rosa_chinensis RchiOBHm_Chr2g0175591
rosa_laevigata RLG00000022342
rosa_multiflora Rmu_sc0013970.1_g000001
rosa_roxburghii Rroxscaffold_2G00077190
rosa_rugosa Rorug02G0586600
rosa_samantha Rh2AG665600 Rh2BG676500 Rh2CG639700 Rh2DG690500
rosa_wichuraiana Rw2G054580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 25
AccI GTMKAC 1 cut(s) 634
AccII CGCG 8 cut(s) 153, 289, 314, 675, 698, 731, 743, 959
AccIII TCCGGA 1 cut(s) 521
AciI CCGC 5 cut(s) 23, 120, 151, 698, 794
AclWI GGATC 1 cut(s) 461
AcsI RAATTY 1 cut(s) 889
AcuI CTGAAG 1 cut(s) 569
AcvI CACGTG 1 cut(s) 602
AfaI GTAC 4 cut(s) 92, 237, 821, 833
AfiI CCNNNNNNNGG 1 cut(s) 157
AflIII ACRYGT 3 cut(s) 199, 673, 904
AgsI TTSAA 6 cut(s) 9, 275, 320, 407, 537, 878
AjiI CACGTC 1 cut(s) 202
AjnI CCWGG 1 cut(s) 568
AloI GAACNNNNNNTCC 2 cut(s) 445, 477
AluBI AGCT 3 cut(s) 434, 528, 661
AluI AGCT 3 cut(s) 434, 528, 661
Alw26I GTCTC 3 cut(s) 173, 283, 350
AlwI GGATC 1 cut(s) 461
AlwNI CAGNNNCTG 1 cut(s) 51
Aor13HI TCCGGA 1 cut(s) 521
ApeKI GCWGC 1 cut(s) 218
ApoI RAATTY 1 cut(s) 889
Asp700I GAANNNNTTC 1 cut(s) 893
AspLEI GCGC 5 cut(s) 289, 733, 743, 814, 959
AspS9I GGNCC 1 cut(s) 117
AsuHPI GGTGA 2 cut(s) 191, 923
AvaII GGWCC 1 cut(s) 117
BbrPI CACGTG 1 cut(s) 602
BbsI GAAGAC 1 cut(s) 967
BbvI GCAGC 1 cut(s) 205
BccI CCATC 1 cut(s) 853
BceAI ACGGC 1 cut(s) 970
BciT130I CCWGG 1 cut(s) 570
BcoDI GTCTC 3 cut(s) 173, 283, 350
BfaI CTAG 2 cut(s) 239, 939
BglII AGATCT 2 cut(s) 78, 133
BisI GCNGC 2 cut(s) 23, 219
BlpI GCTNAGC 1 cut(s) 662
BlsI GCNGC 2 cut(s) 24, 220
Bme1390I CCNGG 1 cut(s) 570
Bme18I GGWCC 1 cut(s) 117
BmgBI CACGTC 1 cut(s) 202
BmgT120I GGNCC 1 cut(s) 117
BmrFI CCNGG 1 cut(s) 570
BmrI ACTGGG 1 cut(s) 166
BmsI GCATC 2 cut(s) 116, 230
BmuI ACTGGG 1 cut(s) 166
BpiI GAAGAC 1 cut(s) 967
BplI GAGNNNNNCTC 2 cut(s) 375, 407
Bpu1102I GCTNAGC 1 cut(s) 662
BsaAI YACGTR 1 cut(s) 602
BsaWI WCCGGW 1 cut(s) 521
Bsc4I CCNNNNNNNGG 1 cut(s) 157
Bse1I ACTGG 1 cut(s) 161
BseAI TCCGGA 1 cut(s) 521
BseBI CCWGG 1 cut(s) 570
BseGI GGATG 3 cut(s) 324, 759, 864
BseLI CCNNNNNNNGG 1 cut(s) 157
BseMII CTCAG 2 cut(s) 273, 479
BseNI ACTGG 1 cut(s) 161
BseXI GCAGC 1 cut(s) 205
Bsh1236I CGCG 8 cut(s) 153, 289, 314, 675, 698, 731, 743, 959
Bsh1285I CGRYCG 1 cut(s) 702
BsiEI CGRYCG 1 cut(s) 702
BsiSI CCGG 1 cut(s) 522
BslI CCNNNNNNNGG 1 cut(s) 157
BsmAI GTCTC 3 cut(s) 173, 283, 350
Bsp13I TCCGGA 1 cut(s) 521
Bsp1407I TGTACA 1 cut(s) 90
Bsp143I GATC 6 cut(s) 78, 133, 309, 385, 453, 594
Bsp1720I GCTNAGC 1 cut(s) 662
Bsp68I TCGCGA 1 cut(s) 314
BspACI CCGC 5 cut(s) 23, 120, 151, 698, 794
BspCNI CTCAG 2 cut(s) 274, 478
BspEI TCCGGA 1 cut(s) 521
BspFNI CGCG 8 cut(s) 153, 289, 314, 675, 698, 731, 743, 959
BspPI GGATC 1 cut(s) 461
BspQI GCTCTTC 1 cut(s) 12
BsrBI CCGCTC 1 cut(s) 25
BsrGI TGTACA 1 cut(s) 90
BsrI ACTGG 1 cut(s) 161
BssMI GATC 6 cut(s) 78, 133, 309, 385, 453, 594
Bst2UI CCWGG 1 cut(s) 570
Bst4CI ACNGT 7 cut(s) 167, 179, 302, 461, 620, 761, 836
Bst6I CTCTTC 1 cut(s) 12
BstAUI TGTACA 1 cut(s) 90
BstBAI YACGTR 1 cut(s) 602
BstC8I GCNNGC 2 cut(s) 291, 499
BstDEI CTNAG 3 cut(s) 282, 465, 662
BstF5I GGATG 3 cut(s) 324, 759, 864
BstFNI CGCG 8 cut(s) 153, 289, 314, 675, 698, 731, 743, 959
BstHHI GCGC 5 cut(s) 289, 733, 743, 814, 959
BstKTI GATC 6 cut(s) 81, 136, 312, 388, 456, 597
BstMAI GTCTC 3 cut(s) 173, 283, 350
BstMBI GATC 6 cut(s) 78, 133, 309, 385, 453, 594
BstMCI CGRYCG 1 cut(s) 702
BstMWI GCNNNNNNNGC 6 cut(s) 10, 19, 215, 431, 440, 672
BstNI CCWGG 1 cut(s) 570
BstSCI CCNGG 1 cut(s) 568
BstUI CGCG 8 cut(s) 153, 289, 314, 675, 698, 731, 743, 959
BstV1I GCAGC 1 cut(s) 205
BstV2I GAAGAC 1 cut(s) 967
BstX2I RGATCY 2 cut(s) 78, 133
BstXI CCANNNNNNTGG 1 cut(s) 81
BstYI RGATCY 2 cut(s) 78, 133
BtgZI GCGATG 1 cut(s) 472
BtrI CACGTC 1 cut(s) 202
BtsCI GGATG 3 cut(s) 324, 759, 864
BtsIMutI CAGTG 1 cut(s) 616
BtuMI TCGCGA 1 cut(s) 314
Cac8I GCNNGC 2 cut(s) 291, 499
CaiI CAGNNNCTG 1 cut(s) 51
CfoI GCGC 5 cut(s) 289, 733, 743, 814, 959
Cfr13I GGNCC 1 cut(s) 117
CseI GACGC 4 cut(s) 409, 490, 704, 737
Csp6I GTAC 4 cut(s) 91, 236, 820, 832
CviAII CATG 3 cut(s) 262, 565, 971
CviJI RGCY 9 cut(s) 22, 101, 434, 443, 528, 547, 661, 802, 926
CviKI_1 RGCY 9 cut(s) 22, 101, 434, 443, 528, 547, 661, 802, 926
CviQI GTAC 4 cut(s) 91, 236, 820, 832
DdeI CTNAG 3 cut(s) 282, 465, 662
DpnI GATC 6 cut(s) 80, 135, 311, 387, 455, 596
DpnII GATC 6 cut(s) 78, 133, 309, 385, 453, 594
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Eco47I GGWCC 1 cut(s) 117
Eco57I CTGAAG 1 cut(s) 569
Eco72I CACGTG 1 cut(s) 602
EcoRII CCWGG 1 cut(s) 568
FaeI CATG 3 cut(s) 265, 568, 974
FaiI YATR 9 cut(s) 56, 263, 342, 363, 446, 566, 780, 935, 972
FatI CATG 3 cut(s) 261, 564, 970
FblI GTMKAC 1 cut(s) 634
Fnu4HI GCNGC 2 cut(s) 23, 219
FokI GGATG 3 cut(s) 311, 766, 871
Fsp4HI GCNGC 2 cut(s) 23, 219
FspBI CTAG 2 cut(s) 239, 939
GlaI GCGC 5 cut(s) 288, 732, 742, 813, 958
GluI GCNGC 2 cut(s) 23, 219
HapII CCGG 1 cut(s) 522
HgaI GACGC 4 cut(s) 409, 490, 704, 737
HhaI GCGC 5 cut(s) 289, 733, 743, 814, 959
Hin1II CATG 3 cut(s) 265, 568, 974
Hin6I GCGC 5 cut(s) 287, 731, 741, 812, 957
HinP1I GCGC 5 cut(s) 287, 731, 741, 812, 957
HindIII AAGCTT 2 cut(s) 526, 659
HinfI GANTC 6 cut(s) 48, 231, 244, 483, 561, 772
HpaII CCGG 1 cut(s) 522
HphI GGTGA 2 cut(s) 191, 923
Hpy166II GTNNAC 4 cut(s) 182, 614, 635, 832
Hpy188I TCNGA 5 cut(s) 226, 268, 283, 334, 468
Hpy188III TCNNGA 8 cut(s) 115, 307, 313, 353, 522, 709, 898, 983
Hpy8I GTNNAC 4 cut(s) 182, 614, 635, 832
Hpy99I CGWCG 7 cut(s) 179, 425, 461, 506, 698, 731, 989
HpyAV CCTTC 5 cut(s) 15, 359, 500, 602, 820
HpyCH4III ACNGT 7 cut(s) 167, 179, 302, 461, 620, 761, 836
HpyCH4IV ACGT 4 cut(s) 184, 201, 601, 904
HpyCH4V TGCA 1 cut(s) 221
HpyF10VI GCNNNNNNNGC 6 cut(s) 10, 19, 215, 431, 440, 672
HpyF3I CTNAG 3 cut(s) 282, 465, 662
HpySE526I ACGT 4 cut(s) 184, 201, 601, 904
Hsp92II CATG 3 cut(s) 265, 568, 974
HspAI GCGC 5 cut(s) 287, 731, 741, 812, 957
Kpn2I TCCGGA 1 cut(s) 521
Kzo9I GATC 6 cut(s) 78, 133, 309, 385, 453, 594
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 1 cut(s) 30
Lsp1109I GCAGC 1 cut(s) 205
LweI GCATC 2 cut(s) 116, 230
MaeI CTAG 2 cut(s) 239, 939
MaeII ACGT 4 cut(s) 184, 201, 601, 904
MaeIII GTNAC 4 cut(s) 185, 602, 838, 950
MalI GATC 6 cut(s) 80, 135, 311, 387, 455, 596
MbiI CCGCTC 1 cut(s) 25
MboI GATC 6 cut(s) 78, 133, 309, 385, 453, 594
MflI RGATCY 2 cut(s) 78, 133
MluCI AATT 3 cut(s) 63, 590, 889
MluI ACGCGT 1 cut(s) 673
MlyI GAGTC 2 cut(s) 555, 781
MnlI CCTC 3 cut(s) 215, 277, 551
MroI TCCGGA 1 cut(s) 521
MroXI GAANNNNTTC 1 cut(s) 893
MseI TTAA 3 cut(s) 575, 945, 997
MspI CCGG 1 cut(s) 522
MspR9I CCNGG 1 cut(s) 570
MvaI CCWGG 1 cut(s) 570
MvnI CGCG 8 cut(s) 153, 289, 314, 675, 698, 731, 743, 959
MwoI GCNNNNNNNGC 6 cut(s) 10, 19, 215, 431, 440, 672
NdeII GATC 6 cut(s) 78, 133, 309, 385, 453, 594
NlaIII CATG 3 cut(s) 265, 568, 974
NmuCI GTSAC 2 cut(s) 185, 602
NruI TCGCGA 1 cut(s) 314
PciSI GCTCTTC 1 cut(s) 12
PcsI WCGNNNNNNNCGW 1 cut(s) 501
PdmI GAANNNNTTC 1 cut(s) 893
PfeI GAWTC 4 cut(s) 48, 231, 244, 483
PflFI GACNNNGTC 1 cut(s) 698
PkrI GCNGC 2 cut(s) 24, 220
PleI GAGTC 2 cut(s) 555, 780
PmaCI CACGTG 1 cut(s) 602
PmlI CACGTG 1 cut(s) 602
PpsI GAGTC 2 cut(s) 555, 780
Ppu21I YACGTR 1 cut(s) 602
Psp6I CCWGG 1 cut(s) 568
PspCI CACGTG 1 cut(s) 602
PspGI CCWGG 1 cut(s) 568
PspPI GGNCC 1 cut(s) 117
PstNI CAGNNNCTG 1 cut(s) 51
PsuI RGATCY 2 cut(s) 78, 133
PsyI GACNNNGTC 1 cut(s) 698
RruI TCGCGA 1 cut(s) 314
RsaI GTAC 4 cut(s) 92, 237, 821, 833
RsaNI GTAC 4 cut(s) 91, 236, 820, 832
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 3 cut(s) 575, 945, 997
SatI GCNGC 2 cut(s) 23, 219
Sau3AI GATC 6 cut(s) 78, 133, 309, 385, 453, 594
Sau96I GGNCC 1 cut(s) 117
SchI GAGTC 2 cut(s) 555, 781
ScrFI CCNGG 1 cut(s) 570
SfaNI GCATC 2 cut(s) 116, 230
SinI GGWCC 1 cut(s) 117
Sse9I AATT 3 cut(s) 63, 590, 889
SsiI CCGC 5 cut(s) 23, 120, 151, 698, 794
SspMI CTAG 2 cut(s) 239, 939
StyD4I CCNGG 1 cut(s) 568
TaaI ACNGT 7 cut(s) 167, 179, 302, 461, 620, 761, 836
TaiI ACGT 4 cut(s) 187, 204, 604, 907
TaqI TCGA 8 cut(s) 354, 456, 504, 702, 708, 852, 897, 984
TasI AATT 3 cut(s) 63, 590, 889
TatI WGTACW 1 cut(s) 90
TauI GCSGC 1 cut(s) 25
TfiI GAWTC 4 cut(s) 48, 231, 244, 483
Tru1I TTAA 3 cut(s) 575, 945, 997
Tru9I TTAA 3 cut(s) 575, 945, 997
TscAI CASTG 1 cut(s) 623
TseFI GTSAC 2 cut(s) 185, 602
TseI GCWGC 1 cut(s) 218
Tsp45I GTSAC 2 cut(s) 185, 602
TspDTI ATGAA 4 cut(s) 181, 250, 461, 770
TspGWI ACGGA 4 cut(s) 243, 726, 837, 919
TspRI CASTG 1 cut(s) 623
Tth111I GACNNNGTC 1 cut(s) 698
VpaK11BI GGWCC 1 cut(s) 117
XapI RAATTY 1 cut(s) 889
XmiI GTMKAC 1 cut(s) 634
XmnI GAANNNNTTC 1 cut(s) 893
XspI CTAG 2 cut(s) 239, 939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.