Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0013930

Physical Location & Seq

Genomic Coordinates Reverse (-)

30046253 .. 30047309

Gene Structure 1057 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1139000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 990 bp

ATGCCTGCCTTCCCAGAAGAGCCGCTGCTAGCTCCGAACCCGGACCGCTTCTGTATGTTTCCGATCCAGTACCCGGAAATCTGGGAAATGTACAAGAAGGCCGAGGCCTCCTTCTGGACCGCCGAGGAGGTCGACCTATGCCAGGACATCCGCCACTGGGAGGCCCTATCCGACGGCGAGAGGCACTTCGTCTCGCACATTCTCGCCTTCTTCGCTGCCTCCGACGGCATCGTCCTTGAGAATCTCGCTGGGAGGTTTATGAAGGAGGTCCAGGTCGCCGAGGCCCGCGCCTTCTACGGGTTCCAGATAGCCATTGAGAACATCCACTCCGAGATGTACAGCCTCCTTCTCGAGACGTACATCAAGGACTCCGACGAGAAGCACCGCCTCTTCCACGCGATCGACACCATCCCCTGCGTCGCGAAGAAGGCCCAGTGGGCCCTGAGATGGATCGACGGCTCCGAGTCTTTCGCGGAGCGAATCGTGGCCTTTGCCTGCGTGGAGGGGATTTTCTTCTCGGGGAGTTTCTGCGCGATATTTTGGCTTAAAAAGCGCGGTTTAATGCCGGGACTGACTTTCTCCAACGAACTGATCTCGCGAGACGAGGGCTTGCACTGCGACTTCGCGTGTTTGCTGTACTCGCTGCTGAGGAAGAAGCTGAGCGAGGATCGGGTTAAGTCGATCGTACGGAACGCGGTGGAGATCGAGAGGGAGTTCGTGTGCGACGCGCTGCCGTGTGCGCTGGTGGGTATGAACGGCGATCTGATGGGCACGTACATCGAGTTCGTGGCGGATCGGCTGCTGGGCTCGTTGGGATACGGCAAGATGTACAATGTCCAGAACCCGTTCGATTGGATGGAGCTGATCAGCCTGCAGGGGAAGACCAACTTCTTCGAGAAGCGCGTCGGCGAGTATCAGAAGGCGTCGGTGATGAACAGCATCAACGGCAACGGTGGCTCCCATGAGTTCAAGATGGACGAGGATTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006139 GO:0006259 GO:0006260 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0009058 GO:0009059 GO:0009987 GO:0012501 GO:0032501 GO:0032502 GO:0033554 GO:0034641 GO:0034645 GO:0043170 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0046483 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0051726 GO:0065007 GO:0071704 GO:0090304 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko00480 ko00983 ko01100 ko04115 map00230 map00240 map00480 map00983 map01100 map04115

Pfam Domains

Protein Families

Ribonuc_red_sm

Protein Analysis

330

Amino Acids

37.67

Weight (kDa)

4.99

Isoelectric Point (pI)

40.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ribonuc_red_sm	PF00268	16 - 282	1.9e-115	Ribonucleotide reductase, small chain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013930)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27060
fragaria_vesca	FvH4_6g53560
malus_domestica	MD00G1139000.v1.1 MD09G1010500.v1.1
prunus_persica	Prupe.3G306600_v2.0.a1
pyrus_communis	pycom111g00840 pycom17g00920
rosa_chinensis	RchiOBHm_Chr2g0175591
rosa_laevigata	RLG00000022342
rosa_multiflora	Rmu_sc0013970.1_g000001
rosa_roxburghii	Rroxscaffold_2G00077190
rosa_rugosa	Rorug02G0586600
rosa_samantha	Rh2AG665600 Rh2BG676500 Rh2CG639700 Rh2DG690500
rosa_wichuraiana	Rw2G054580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	230
AccI	GTMKAC	1 cut(s)	132
AclWI	GGATC	4 cut(s)	58, 458, 675, 801
AcyI	GRCGYC	1 cut(s)	923
AfaI	GTAC	8 cut(s)	71, 92, 338, 359, 638, 687, 776, 830
AfiI	CCNNNNNNNGG	7 cut(s)	73, 114, 142, 157, 160, 297, 447
AgsI	TTSAA	1 cut(s)	970
AjnI	CCWGG	2 cut(s)	141, 270
AloI	GAACNNNNNNTCC	2 cut(s)	311, 343
AluBI	AGCT	3 cut(s)	32, 658, 862
AluI	AGCT	3 cut(s)	32, 658, 862
Alw26I	GTCTC	3 cut(s)	196, 347, 594
AlwI	GGATC	4 cut(s)	58, 458, 675, 801
Ama87I	CYCGRG	2 cut(s)	350, 517
AoxI	GGCC	7 cut(s)	99, 105, 162, 282, 429, 438, 486
ApaI	GGGCCC	1 cut(s)	442
ApeKI	GCWGC	5 cut(s)	25, 215, 643, 730, 799
Asp700I	GAANNNNTTC	1 cut(s)	845
AspLEI	GCGC	6 cut(s)	290, 533, 555, 730, 742, 903
AspS9I	GGNCC	8 cut(s)	43, 117, 163, 268, 283, 430, 438, 439
AsuC2I	CCSGG	3 cut(s)	41, 74, 567
AsuHPI	GGTGA	1 cut(s)	940
AsuNHI	GCTAGC	1 cut(s)	28
AvaI	CYCGRG	2 cut(s)	350, 517
AvaII	GGWCC	3 cut(s)	43, 117, 268
BaeGI	GKGCMC	2 cut(s)	442, 773
BanII	GRGCYC	2 cut(s)	442, 809
BbsI	GAAGAC	1 cut(s)	887
BbvCI	CCTCAGC	1 cut(s)	647
BbvI	GCAGC	5 cut(s)	12, 202, 630, 717, 786
BccI	CCATC	5 cut(s)	416, 441, 760, 850, 967
BceAI	ACGGC	7 cut(s)	190, 241, 472, 718, 772, 835, 961
BcgI	CGANNNNNNTGC	2 cut(s)	760, 794
BciT130I	CCWGG	2 cut(s)	143, 272
BciVI	GTATCC	1 cut(s)	809
BclI	TGATCA	1 cut(s)	864
BcnI	CCSGG	3 cut(s)	41, 74, 567
BcoDI	GTCTC	3 cut(s)	196, 347, 594
BfaI	CTAG	1 cut(s)	29
BfmI	CTRYAG	1 cut(s)	872
BfuI	GTATCC	1 cut(s)	809
BglI	GCCNNNNNGGC	1 cut(s)	437
BisI	GCNGC	6 cut(s)	23, 26, 216, 644, 731, 800
BlpI	GCTNAGC	1 cut(s)	659
BlsI	GCNGC	6 cut(s)	24, 27, 217, 645, 732, 801
Bme1390I	CCNGG	5 cut(s)	41, 74, 143, 272, 567
Bme18I	GGWCC	3 cut(s)	43, 117, 268
BmeT110I	CYCGRG	2 cut(s)	350, 517
BmgT120I	GGNCC	8 cut(s)	43, 117, 163, 268, 283, 430, 438, 439
BmiI	GGNNCC	4 cut(s)	302, 440, 460, 958
BmrFI	CCNGG	5 cut(s)	41, 74, 143, 272, 567
BmrI	ACTGGG	2 cut(s)	166, 427
BmsI	GCATC	2 cut(s)	237, 948
BmtI	GCTAGC	1 cut(s)	32
BmuI	ACTGGG	2 cut(s)	166, 427
BpiI	GAAGAC	1 cut(s)	887
Bpu10I	CCTNAGC	1 cut(s)	647
Bpu1102I	GCTNAGC	1 cut(s)	659
BpuEI	CTTGAG	1 cut(s)	257
BpuMI	CCSGG	3 cut(s)	41, 74, 567
BsaAI	YACGTR	1 cut(s)	774
BsaHI	GRCGYC	1 cut(s)	923
BsaJI	CCNNGG	3 cut(s)	102, 123, 279
BsaXI	ACNNNNNCTCC	6 cut(s)	311, 341, 704, 734, 941, 971
Bsc4I	CCNNNNNNNGG	7 cut(s)	73, 114, 142, 157, 160, 297, 447
Bse1I	ACTGG	3 cut(s)	67, 161, 433
BseBI	CCWGG	2 cut(s)	143, 272
BseDI	CCNNGG	3 cut(s)	102, 123, 279
BseGI	GGATG	4 cut(s)	147, 321, 408, 861
BseLI	CCNNNNNNNGG	7 cut(s)	73, 114, 142, 157, 160, 297, 447
BseMII	CTCAG	3 cut(s)	434, 638, 650
BseNI	ACTGG	3 cut(s)	67, 161, 433
BseRI	GAGGAG	1 cut(s)	140
BseSI	GKGCMC	2 cut(s)	442, 773
BseXI	GCAGC	5 cut(s)	12, 202, 630, 717, 786
BseYI	CCCAGC	2 cut(s)	248, 802
Bsh1285I	CGRYCG	2 cut(s)	402, 684
BshFI	GGCC	7 cut(s)	101, 107, 164, 284, 431, 440, 488
BsiEI	CGRYCG	2 cut(s)	402, 684
BsiHKCI	CYCGRG	2 cut(s)	350, 517
BsiSI	CCGG	3 cut(s)	41, 74, 566
BsiWI	CGTACG	1 cut(s)	685
BslFI	GGGAC	1 cut(s)	582
BslI	CCNNNNNNNGG	7 cut(s)	73, 114, 142, 157, 160, 297, 447
BsmAI	GTCTC	3 cut(s)	196, 347, 594
BsmBI	CGTCTC	3 cut(s)	196, 347, 594
BsmFI	GGGAC	1 cut(s)	582
BsnI	GGCC	7 cut(s)	101, 107, 164, 284, 431, 440, 488
BsoBI	CYCGRG	2 cut(s)	350, 517
Bsp120I	GGGCCC	1 cut(s)	438
Bsp1286I	GDGCHC	3 cut(s)	442, 773, 809
Bsp1407I	TGTACA	3 cut(s)	90, 336, 828
Bsp1720I	GCTNAGC	1 cut(s)	659
Bsp68I	TCGCGA	2 cut(s)	422, 598
BspANI	GGCC	7 cut(s)	101, 107, 164, 284, 431, 440, 488
BspCNI	CTCAG	3 cut(s)	435, 639, 651
BspLI	GGNNCC	4 cut(s)	302, 440, 460, 958
BspMAI	CTGCAG	1 cut(s)	876
BspOI	GCTAGC	1 cut(s)	32
BspPI	GGATC	4 cut(s)	58, 458, 675, 801
BspQI	GCTCTTC	1 cut(s)	12
BsrGI	TGTACA	3 cut(s)	90, 336, 828
BsrI	ACTGG	3 cut(s)	67, 161, 433
BssECI	CCNNGG	3 cut(s)	102, 123, 279
BssNI	GRCGYC	1 cut(s)	923
Bst2UI	CCWGG	2 cut(s)	143, 272
Bst4CI	ACNGT	1 cut(s)	953
Bst6I	CTCTTC	2 cut(s)	12, 395
BstACI	GRCGYC	1 cut(s)	923
BstAUI	TGTACA	3 cut(s)	90, 336, 828
BstBAI	YACGTR	1 cut(s)	774
BstC8I	GCNNGC	6 cut(s)	6, 30, 286, 496, 611, 872
BstDEI	CTNAG	3 cut(s)	443, 647, 659
BstENI	CCTNNNNNAGG	1 cut(s)	140
BstF5I	GGATG	4 cut(s)	147, 321, 408, 861
BstHHI	GCGC	6 cut(s)	290, 533, 555, 730, 742, 903
BstMAI	GTCTC	3 cut(s)	196, 347, 594
BstMCI	CGRYCG	2 cut(s)	402, 684
BstMWI	GCNNNNNNNGC	9 cut(s)	212, 428, 437, 550, 615, 640, 739, 945, 954
BstNI	CCWGG	2 cut(s)	143, 272
BstSCI	CCNGG	5 cut(s)	39, 72, 141, 270, 565
BstSFI	CTRYAG	1 cut(s)	872
BstSLI	GKGCMC	2 cut(s)	442, 773
BstV1I	GCAGC	5 cut(s)	12, 202, 630, 717, 786
BstV2I	GAAGAC	1 cut(s)	887
BsuI	GTATCC	1 cut(s)	809
BsuRI	GGCC	7 cut(s)	101, 107, 164, 284, 431, 440, 488
BtsCI	GGATG	4 cut(s)	147, 321, 408, 861
BtsI	GCAGTG	1 cut(s)	613
BtsIMutI	CAGTG	3 cut(s)	154, 440, 613
BtuMI	TCGCGA	2 cut(s)	422, 598
Cac8I	GCNNGC	6 cut(s)	6, 30, 286, 496, 611, 872
CfoI	GCGC	6 cut(s)	290, 533, 555, 730, 742, 903
Cfr13I	GGNCC	8 cut(s)	43, 117, 163, 268, 283, 430, 438, 439
CpoI	CGGWCCG	1 cut(s)	43
CseI	GACGC	4 cut(s)	406, 734, 892, 912
Csp6I	GTAC	8 cut(s)	70, 91, 337, 358, 637, 686, 775, 829
CspI	CGGWCCG	1 cut(s)	43
CviAII	CATG	1 cut(s)	962
CviQI	GTAC	8 cut(s)	70, 91, 337, 358, 637, 686, 775, 829
DdeI	CTNAG	3 cut(s)	443, 647, 659
DrdI	GACNNNNNNGTC	1 cut(s)	230
DseDI	GACNNNNNNGTC	1 cut(s)	230
Eam1104I	CTCTTC	2 cut(s)	12, 395
EarI	CTCTTC	2 cut(s)	12, 395
EciI	GGCGGA	2 cut(s)	140, 806
Eco147I	AGGCCT	1 cut(s)	107
Eco24I	GRGCYC	2 cut(s)	442, 809
Eco47I	GGWCC	3 cut(s)	43, 117, 268
Eco88I	CYCGRG	2 cut(s)	350, 517
EcoNI	CCTNNNNNAGG	1 cut(s)	140
EcoO109I	RGGNCCY	2 cut(s)	163, 439
EcoRII	CCWGG	2 cut(s)	141, 270
EcoT38I	GRGCYC	2 cut(s)	442, 809
Esp3I	CGTCTC	3 cut(s)	196, 347, 594
FaeI	CATG	1 cut(s)	965
FaiI	YATR	5 cut(s)	56, 139, 260, 752, 963
FalI	AAGNNNNNCTT	2 cut(s)	872, 904
FaqI	GGGAC	1 cut(s)	582
FatI	CATG	1 cut(s)	961
FauI	CCCGC	1 cut(s)	293
FbaI	TGATCA	1 cut(s)	864
FblI	GTMKAC	1 cut(s)	132
Fnu4HI	GCNGC	6 cut(s)	23, 26, 216, 644, 731, 800
FokI	GGATG	4 cut(s)	134, 308, 395, 868
FriOI	GRGCYC	2 cut(s)	442, 809
Fsp4HI	GCNGC	6 cut(s)	23, 26, 216, 644, 731, 800
FspBI	CTAG	1 cut(s)	29
GlaI	GCGC	6 cut(s)	289, 532, 554, 729, 741, 902
GluI	GCNGC	6 cut(s)	23, 26, 216, 644, 731, 800
GsaI	CCCAGC	2 cut(s)	252, 806
HaeIII	GGCC	7 cut(s)	101, 107, 164, 284, 431, 440, 488
HapII	CCGG	3 cut(s)	41, 74, 566
HgaI	GACGC	4 cut(s)	406, 734, 892, 912
HhaI	GCGC	6 cut(s)	290, 533, 555, 730, 742, 903
Hin1I	GRCGYC	1 cut(s)	923
Hin1II	CATG	1 cut(s)	965
Hin6I	GCGC	6 cut(s)	288, 531, 553, 728, 740, 901
HinP1I	GCGC	6 cut(s)	288, 531, 553, 728, 740, 901
HincII	GTYRAC	1 cut(s)	133
HindII	GTYRAC	1 cut(s)	133
HinfI	GANTC	4 cut(s)	241, 368, 464, 480
HpaII	CCGG	3 cut(s)	41, 74, 566
HphI	GGTGA	1 cut(s)	940
Hpy166II	GTNNAC	1 cut(s)	133
Hpy8I	GTNNAC	1 cut(s)	133
Hpy99I	CGWCG	8 cut(s)	176, 227, 377, 422, 458, 728, 908, 928
HpyAV	CCTTC	9 cut(s)	19, 91, 121, 217, 256, 301, 356, 421, 913
HpyCH4III	ACNGT	1 cut(s)	953
HpyCH4IV	ACGT	2 cut(s)	356, 773
HpyCH4V	TGCA	2 cut(s)	613, 874
HpyF10VI	GCNNNNNNNGC	9 cut(s)	212, 428, 437, 550, 615, 640, 739, 945, 954
HpyF3I	CTNAG	3 cut(s)	443, 647, 659
HpySE526I	ACGT	2 cut(s)	356, 773
Hsp92I	GRCGYC	1 cut(s)	923
Hsp92II	CATG	1 cut(s)	965
HspAI	GCGC	6 cut(s)	288, 531, 553, 728, 740, 901
Ksp22I	TGATCA	1 cut(s)	864
LguI	GCTCTTC	1 cut(s)	12
LmnI	GCTCC	5 cut(s)	37, 464, 475, 859, 962
Lsp1109I	GCAGC	5 cut(s)	12, 202, 630, 717, 786
LweI	GCATC	2 cut(s)	237, 948
MaeI	CTAG	1 cut(s)	29
MaeII	ACGT	2 cut(s)	356, 773
MboII	GAAGA	8 cut(s)	29, 202, 382, 436, 505, 664, 883, 892
MhlI	GDGCHC	3 cut(s)	442, 773, 809
MlyI	GAGTC	2 cut(s)	362, 473
MmeI	TCCRAC	4 cut(s)	195, 246, 396, 606
MroXI	GAANNNNTTC	1 cut(s)	845
MseI	TTAA	3 cut(s)	546, 560, 675
MspA1I	CMGCKG	1 cut(s)	25
MspI	CCGG	3 cut(s)	41, 74, 566
MspR9I	CCNGG	5 cut(s)	41, 74, 143, 272, 567
MvaI	CCWGG	2 cut(s)	143, 272
MwoI	GCNNNNNNNGC	9 cut(s)	212, 428, 437, 550, 615, 640, 739, 945, 954
NciI	CCSGG	3 cut(s)	41, 74, 567
NheI	GCTAGC	1 cut(s)	28
NlaIII	CATG	1 cut(s)	965
NlaIV	GGNNCC	4 cut(s)	302, 440, 460, 958
NmeAIII	GCCGAG	3 cut(s)	127, 148, 304
NruI	TCGCGA	2 cut(s)	422, 598
PaeR7I	CTCGAG	1 cut(s)	350
PceI	AGGCCT	1 cut(s)	107
PciSI	GCTCTTC	1 cut(s)	12
PcsI	WCGNNNNNNNCGW	3 cut(s)	219, 459, 900
PdmI	GAANNNNTTC	1 cut(s)	845
PfeI	GAWTC	2 cut(s)	241, 480
Pfl23II	CGTACG	1 cut(s)	685
PkrI	GCNGC	6 cut(s)	24, 27, 217, 645, 732, 801
Ple19I	CGATCG	2 cut(s)	402, 684
PleI	GAGTC	2 cut(s)	362, 472
PpsI	GAGTC	2 cut(s)	362, 472
Ppu21I	YACGTR	1 cut(s)	774
Psp6I	CCWGG	2 cut(s)	141, 270
PspFI	CCCAGC	2 cut(s)	248, 802
PspGI	CCWGG	2 cut(s)	141, 270
PspLI	CGTACG	1 cut(s)	685
PspN4I	GGNNCC	4 cut(s)	302, 440, 460, 958
PspOMI	GGGCCC	1 cut(s)	438
PspPI	GGNCC	8 cut(s)	43, 117, 163, 268, 283, 430, 438, 439
PsrI	GAACNNNNNNTAC	2 cut(s)	767, 799
PstI	CTGCAG	1 cut(s)	876
PvuI	CGATCG	2 cut(s)	402, 684
RruI	TCGCGA	2 cut(s)	422, 598
RsaI	GTAC	8 cut(s)	71, 92, 338, 359, 638, 687, 776, 830
RsaNI	GTAC	8 cut(s)	70, 91, 337, 358, 637, 686, 775, 829
Rsr2I	CGGWCCG	1 cut(s)	43
RsrII	CGGWCCG	1 cut(s)	43
SalI	GTCGAC	1 cut(s)	131
SapI	GCTCTTC	1 cut(s)	12
SaqAI	TTAA	3 cut(s)	546, 560, 675
SatI	GCNGC	6 cut(s)	23, 26, 216, 644, 731, 800
Sau96I	GGNCC	8 cut(s)	43, 117, 163, 268, 283, 430, 438, 439
SbfI	CCTGCAGG	1 cut(s)	876
SchI	GAGTC	2 cut(s)	362, 473
ScrFI	CCNGG	5 cut(s)	41, 74, 143, 272, 567
SdaI	CCTGCAGG	1 cut(s)	876
SduI	GDGCHC	3 cut(s)	442, 773, 809
SfaNI	GCATC	2 cut(s)	237, 948
SfcI	CTRYAG	1 cut(s)	872
SfiI	GGCCNNNNNGGCC	1 cut(s)	437
Sfr274I	CTCGAG	1 cut(s)	350
SinI	GGWCC	3 cut(s)	43, 117, 268
SlaI	CTCGAG	1 cut(s)	350
SmlI	CTYRAG	2 cut(s)	236, 350
SmoI	CTYRAG	2 cut(s)	236, 350
Sse8387I	CCTGCAGG	1 cut(s)	876
SseBI	AGGCCT	1 cut(s)	107
SspMI	CTAG	1 cut(s)	29
StuI	AGGCCT	1 cut(s)	107
StyD4I	CCNGG	5 cut(s)	39, 72, 141, 270, 565
TaaI	ACNGT	1 cut(s)	953
TaiI	ACGT	2 cut(s)	359, 776
TaqI	TCGA	9 cut(s)	132, 351, 402, 453, 680, 705, 780, 849, 894
TatI	WGTACW	4 cut(s)	90, 336, 636, 828
TauI	GCSGC	1 cut(s)	25
TfiI	GAWTC	2 cut(s)	241, 480
Tru1I	TTAA	3 cut(s)	546, 560, 675
Tru9I	TTAA	3 cut(s)	546, 560, 675
TscAI	CASTG	3 cut(s)	161, 440, 620
TseI	GCWGC	5 cut(s)	25, 215, 643, 730, 799
TspDTI	ATGAA	3 cut(s)	275, 767, 947
TspGWI	ACGGA	1 cut(s)	703
TspRI	CASTG	3 cut(s)	161, 440, 620
VpaK11BI	GGWCC	3 cut(s)	43, 117, 268
XagI	CCTNNNNNAGG	1 cut(s)	140
XhoI	CTCGAG	1 cut(s)	350
XmiI	GTMKAC	1 cut(s)	132
XmnI	GAANNNNTTC	1 cut(s)	845
XspI	CTAG	1 cut(s)	29

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.