pycom111g00840

Ribonucleoside-diphosphate reductase small

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0013930
Physical Location & Seq
Reverse (-)
561004 .. 562351
1348 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g00840.1

Sequence Viewer

Length: 1224 bp
ATGACCGTTACACCCCTCTTCACAGTTCACAGTTCACAGTTCACACCATCCTCATCCGGTAGCCGCAAACGCAAACCACCGAAATCGGACTGCGCGCCTATTCACCCTCCTGAATTTCCCAAAGTACCCTCGTGTTCGGTTCATTCTCCCGCAAATTCACAAAATTTGATTTCGTTTCTGAAAAATCCCAAATCGAGTTTCATCACAAAAATGCCTGCCTTCCCAGAAGAGCCGCTGCTAGCCCCGAACCCGGACCGCTTCTGCATGTTCCCAATCCAGTACCCTGAAATCTGGGAAATGTACAAGAAGGCGGAAGCCTCCTTCTGGACCGCCGAGGAGGTCGACCTTTGCCAGGACGTCCGCCACTGGGAAGCTCTATCCGACGGCGAGAGGCACTTCGTCTCGCACATCCTCGCCTTCTTCGCCGCTTCCGATGGAATTGTCCTGGAGAATCTCGCCGGAAGGTTCATGAAGGAGGTCCAGGTCGCCGAGGCCCGCGCCTTCTATGGGTTCCAGATCGCCATTGAGAACATCCACTCCGAGATGTACAGCCTCCTTCTCGAGACTTACATCAAGGACTCCGACGAGAAGCACCGCCTCTTCCACGCGATCGACACCATCCTCTGCGTCACGAAGAAGGCCCAGTGGGCCCTGAGATCGATCGACGGCTCTGAGTCTTTCGCGGAGCGAATCGTGGCCTTTGCCTGCGTGGAAGGGATTTTCTTCTCTGGGAGTTTTTGCGCGATATTTTGGCTAAAAAAGCGCGGTTTAATGCCGGGACTGACTTTCTCGAACGAGCTGATCTCACGAGACGAGGGCTTGCACTGCGACTTCGCGTGTTTGCTGTACTCGCTGCTGAGGAAGAAGCTGAGCGAGGATCGGGTAAAGTCGATAGTTCGGAACGCGGTGGAGATCGAGAAGGAGTTCGTGTGCGACGCGCTGCCTTGTGCCCTGGTGGGGATGAACGGCGATCTGATGAGCACCTACATCGAGTTCGTGGCGGATCGTTTGCTTGGCGCGTTGGGATACGGGAAGAAGTACAACGTCCAGAACCCGTTCGATTGGATGGAGCTGATCAGCCTCCAGGGGAAGACCAACTTCTTCGAGAAACGCGTTGGCGAGTATCAGAAGGCGTCGGTGATGAACAGCATCAACGGCAACGGTGGGTCGCATGAGTTCAAGATGGACGAGGATTTCTGCTTGATCGATCGTCTGATTGAGTGA

Protein Analysis

408

Amino Acids

46.15

Weight (kDa)

5.74

Isoelectric Point (pI)

46.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuc_red_sm PF00268 86 - 352 1.1e-112 Ribonucleotide reductase, small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013930)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27060
fragaria_vesca FvH4_6g53560
malus_domestica MD00G1139000.v1.1 MD09G1010500.v1.1
prunus_persica Prupe.3G306600_v2.0.a1
pyrus_communis pycom111g00840 pycom17g00920
rosa_chinensis RchiOBHm_Chr2g0175591
rosa_laevigata RLG00000022342
rosa_multiflora Rmu_sc0013970.1_g000001
rosa_roxburghii Rroxscaffold_2G00077190
rosa_rugosa Rorug02G0586600
rosa_samantha Rh2AG665600 Rh2BG676500 Rh2CG639700 Rh2DG690500
rosa_wichuraiana Rw2G054580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 360
AccI GTMKAC 1 cut(s) 342
AclWI GGATC 2 cut(s) 885, 1011
AcsI RAATTY 3 cut(s) 113, 154, 163
AcyI GRCGYC 2 cut(s) 357, 1133
AfaI GTAC 6 cut(s) 126, 281, 302, 548, 848, 1040
AfiI CCNNNNNNNGG 6 cut(s) 250, 324, 352, 367, 507, 957
AflIII ACRYGT 1 cut(s) 1111
AgsI TTSAA 1 cut(s) 1180
AjnI CCWGG 5 cut(s) 351, 444, 480, 951, 1083
AjuI GAANNNNNNNTTGG 2 cut(s) 182, 214
AloI GAACNNNNNNTCC 2 cut(s) 521, 553
AluBI AGCT 4 cut(s) 374, 799, 868, 1072
AluI AGCT 4 cut(s) 374, 799, 868, 1072
Alw21I GWGCWC 1 cut(s) 983
Alw26I GTCTC 3 cut(s) 406, 557, 804
AlwI GGATC 2 cut(s) 885, 1011
Ama87I CYCGRG 1 cut(s) 560
AoxI GGCC 4 cut(s) 492, 639, 648, 696
ApaI GGGCCC 1 cut(s) 652
ApeKI GCWGC 3 cut(s) 235, 853, 940
ApoI RAATTY 3 cut(s) 113, 154, 163
Asp700I GAANNNNTTC 2 cut(s) 923, 1055
AspLEI GCGC 7 cut(s) 95, 97, 500, 743, 765, 940, 1019
AspS9I GGNCC 7 cut(s) 253, 327, 478, 493, 640, 648, 649
AsuC2I CCSGG 2 cut(s) 251, 777
AsuHPI GGTGA 2 cut(s) 95, 1150
AsuNHI GCTAGC 1 cut(s) 238
AvaI CYCGRG 1 cut(s) 560
AvaII GGWCC 3 cut(s) 253, 327, 478
BaeGI GKGCMC 2 cut(s) 652, 952
BanII GRGCYC 1 cut(s) 652
BauI CACGAG 2 cut(s) 130, 807
BbsI GAAGAC 1 cut(s) 1097
Bbv12I GWGCWC 1 cut(s) 983
BbvCI CCTCAGC 1 cut(s) 857
BbvI GCAGC 3 cut(s) 222, 840, 927
BccI CCATC 5 cut(s) 55, 428, 626, 1060, 1177
BceAI ACGGC 4 cut(s) 400, 682, 982, 1171
BcgI CGANNNNNNTGC 2 cut(s) 970, 1004
BciT130I CCWGG 5 cut(s) 353, 446, 482, 953, 1085
BciVI GTATCC 1 cut(s) 1019
BclI TGATCA 1 cut(s) 1074
BcnI CCSGG 2 cut(s) 251, 777
BcoDI GTCTC 3 cut(s) 406, 557, 804
BfaI CTAG 1 cut(s) 239
BfuI GTATCC 1 cut(s) 1019
BglI GCCNNNNNGGC 1 cut(s) 647
BisI GCNGC 6 cut(s) 64, 233, 236, 426, 854, 941
BlpI GCTNAGC 1 cut(s) 869
BlsI GCNGC 6 cut(s) 65, 234, 237, 427, 855, 942
Bme1390I CCNGG 7 cut(s) 251, 353, 446, 482, 777, 953, 1085
Bme18I GGWCC 3 cut(s) 253, 327, 478
BmeT110I CYCGRG 1 cut(s) 560
BmgT120I GGNCC 7 cut(s) 253, 327, 478, 493, 640, 648, 649
BmiI GGNNCC 2 cut(s) 512, 650
BmrFI CCNGG 7 cut(s) 251, 353, 446, 482, 777, 953, 1085
BmrI ACTGGG 2 cut(s) 376, 637
BmsI GCATC 1 cut(s) 1158
BmtI GCTAGC 1 cut(s) 242
BmuI ACTGGG 2 cut(s) 376, 637
BpiI GAAGAC 1 cut(s) 1097
BplI GAGNNNNNCTC 2 cut(s) 788, 820
BpmI CTGGAG 2 cut(s) 467, 1067
Bpu10I CCTNAGC 1 cut(s) 857
Bpu1102I GCTNAGC 1 cut(s) 869
BpuMI CCSGG 2 cut(s) 251, 777
Bsa29I ATCGAT 2 cut(s) 659, 1206
BsaHI GRCGYC 2 cut(s) 357, 1133
BsaJI CCNNGG 4 cut(s) 333, 489, 951, 1084
BsaWI WCCGGW 1 cut(s) 56
BsaXI ACNNNNNCTCC 4 cut(s) 521, 551, 914, 944
Bsc4I CCNNNNNNNGG 6 cut(s) 250, 324, 352, 367, 507, 957
Bse1I ACTGG 3 cut(s) 277, 371, 643
BseBI CCWGG 5 cut(s) 353, 446, 482, 953, 1085
BseCI ATCGAT 2 cut(s) 659, 1206
BseDI CCNNGG 4 cut(s) 333, 489, 951, 1084
BseGI GGATG 7 cut(s) 47, 53, 408, 531, 618, 966, 1071
BseLI CCNNNNNNNGG 6 cut(s) 250, 324, 352, 367, 507, 957
BseMII CTCAG 4 cut(s) 644, 663, 848, 860
BseNI ACTGG 3 cut(s) 277, 371, 643
BsePI GCGCGC 1 cut(s) 93
BseRI GAGGAG 1 cut(s) 350
BseSI GKGCMC 2 cut(s) 652, 952
BseXI GCAGC 3 cut(s) 222, 840, 927
Bsh1285I CGRYCG 3 cut(s) 612, 663, 1210
BshFI GGCC 4 cut(s) 494, 641, 650, 698
BshVI ATCGAT 2 cut(s) 659, 1206
BsiEI CGRYCG 3 cut(s) 612, 663, 1210
BsiHKAI GWGCWC 1 cut(s) 983
BsiHKCI CYCGRG 1 cut(s) 560
BsiSI CCGG 4 cut(s) 57, 251, 459, 776
BslFI GGGAC 1 cut(s) 792
BslI CCNNNNNNNGG 6 cut(s) 250, 324, 352, 367, 507, 957
BsmAI GTCTC 3 cut(s) 406, 557, 804
BsmBI CGTCTC 2 cut(s) 406, 804
BsmFI GGGAC 1 cut(s) 792
BsnI GGCC 4 cut(s) 494, 641, 650, 698
BsoBI CYCGRG 1 cut(s) 560
Bsp120I GGGCCC 1 cut(s) 648
Bsp1286I GDGCHC 3 cut(s) 652, 952, 983
Bsp1407I TGTACA 2 cut(s) 300, 546
Bsp1720I GCTNAGC 1 cut(s) 869
BspANI GGCC 4 cut(s) 494, 641, 650, 698
BspCNI CTCAG 4 cut(s) 645, 664, 849, 861
BspDI ATCGAT 2 cut(s) 659, 1206
BspHI TCATGA 1 cut(s) 468
BspLI GGNNCC 2 cut(s) 512, 650
BspOI GCTAGC 1 cut(s) 242
BspPI GGATC 2 cut(s) 885, 1011
BspQI GCTCTTC 1 cut(s) 222
BsrGI TGTACA 2 cut(s) 300, 546
BsrI ACTGG 3 cut(s) 277, 371, 643
BssECI CCNNGG 4 cut(s) 333, 489, 951, 1084
BssHII GCGCGC 1 cut(s) 93
BssNI GRCGYC 2 cut(s) 357, 1133
BssSI CACGAG 2 cut(s) 130, 807
Bst2BI CACGAG 2 cut(s) 130, 807
Bst2UI CCWGG 5 cut(s) 353, 446, 482, 953, 1085
Bst4CI ACNGT 5 cut(s) 7, 25, 32, 39, 1163
Bst6I CTCTTC 3 cut(s) 23, 222, 605
BstACI GRCGYC 2 cut(s) 357, 1133
BstAUI TGTACA 2 cut(s) 300, 546
BstC8I GCNNGC 6 cut(s) 95, 216, 240, 496, 706, 821
BstDEI CTNAG 4 cut(s) 653, 672, 857, 869
BstENI CCTNNNNNAGG 1 cut(s) 350
BstF5I GGATG 7 cut(s) 47, 53, 408, 531, 618, 966, 1071
BstHHI GCGC 7 cut(s) 95, 97, 500, 743, 765, 940, 1019
BstMAI GTCTC 3 cut(s) 406, 557, 804
BstMCI CGRYCG 3 cut(s) 612, 663, 1210
BstMWI GCNNNNNNNGC 7 cut(s) 69, 422, 647, 760, 825, 850, 1155
BstNI CCWGG 5 cut(s) 353, 446, 482, 953, 1085
BstNSI RCATGY 1 cut(s) 268
BstSCI CCNGG 7 cut(s) 249, 351, 444, 480, 775, 951, 1083
BstSLI GKGCMC 2 cut(s) 652, 952
BstV1I GCAGC 3 cut(s) 222, 840, 927
BstV2I GAAGAC 1 cut(s) 1097
Bsu15I ATCGAT 2 cut(s) 659, 1206
BsuI GTATCC 1 cut(s) 1019
BsuRI GGCC 4 cut(s) 494, 641, 650, 698
BsuTUI ATCGAT 2 cut(s) 659, 1206
BtsCI GGATG 7 cut(s) 47, 53, 408, 531, 618, 966, 1071
BtsI GCAGTG 1 cut(s) 823
BtsIMutI CAGTG 3 cut(s) 364, 650, 823
Cac8I GCNNGC 6 cut(s) 95, 216, 240, 496, 706, 821
CciI TCATGA 1 cut(s) 468
CfoI GCGC 7 cut(s) 95, 97, 500, 743, 765, 940, 1019
Cfr13I GGNCC 7 cut(s) 253, 327, 478, 493, 640, 648, 649
ClaI ATCGAT 2 cut(s) 659, 1206
CpoI CGGWCCG 1 cut(s) 253
CseI GACGC 3 cut(s) 616, 944, 1122
Csp6I GTAC 6 cut(s) 125, 280, 301, 547, 847, 1039
CspI CGGWCCG 1 cut(s) 253
CviAII CATG 3 cut(s) 265, 469, 1172
CviQI GTAC 6 cut(s) 125, 280, 301, 547, 847, 1039
DdeI CTNAG 4 cut(s) 653, 672, 857, 869
Eam1104I CTCTTC 3 cut(s) 23, 222, 605
EarI CTCTTC 3 cut(s) 23, 222, 605
EciI GGCGGA 3 cut(s) 326, 350, 1016
Eco24I GRGCYC 1 cut(s) 652
Eco47I GGWCC 3 cut(s) 253, 327, 478
Eco88I CYCGRG 1 cut(s) 560
EcoNI CCTNNNNNAGG 1 cut(s) 350
EcoO109I RGGNCCY 1 cut(s) 649
EcoRII CCWGG 5 cut(s) 351, 444, 480, 951, 1083
EcoT38I GRGCYC 1 cut(s) 652
Esp3I CGTCTC 2 cut(s) 406, 804
FaeI CATG 3 cut(s) 268, 472, 1175
FaiI YATR 4 cut(s) 266, 470, 507, 1173
FalI AAGNNNNNCTT 2 cut(s) 1082, 1114
FaqI GGGAC 1 cut(s) 792
FatI CATG 3 cut(s) 264, 468, 1171
FauI CCCGC 2 cut(s) 157, 503
FbaI TGATCA 1 cut(s) 1074
FblI GTMKAC 1 cut(s) 342
Fnu4HI GCNGC 6 cut(s) 64, 233, 236, 426, 854, 941
FokI GGATG 7 cut(s) 34, 40, 395, 518, 605, 973, 1078
FriOI GRGCYC 1 cut(s) 652
Fsp4HI GCNGC 6 cut(s) 64, 233, 236, 426, 854, 941
FspBI CTAG 1 cut(s) 239
GlaI GCGC 7 cut(s) 94, 96, 499, 742, 764, 939, 1018
GluI GCNGC 6 cut(s) 64, 233, 236, 426, 854, 941
GsuI CTGGAG 2 cut(s) 467, 1067
HaeIII GGCC 4 cut(s) 494, 641, 650, 698
HapII CCGG 4 cut(s) 57, 251, 459, 776
HgaI GACGC 3 cut(s) 616, 944, 1122
HhaI GCGC 7 cut(s) 95, 97, 500, 743, 765, 940, 1019
Hin1I GRCGYC 2 cut(s) 357, 1133
Hin1II CATG 3 cut(s) 268, 472, 1175
Hin6I GCGC 7 cut(s) 93, 95, 498, 741, 763, 938, 1017
HinP1I GCGC 7 cut(s) 93, 95, 498, 741, 763, 938, 1017
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HinfI GANTC 4 cut(s) 451, 578, 674, 690
HpaII CCGG 4 cut(s) 57, 251, 459, 776
HphI GGTGA 2 cut(s) 95, 1150
Hpy166II GTNNAC 4 cut(s) 28, 35, 42, 343
Hpy8I GTNNAC 4 cut(s) 28, 35, 42, 343
Hpy99I CGWCG 5 cut(s) 386, 587, 668, 938, 1138
HpyCH4III ACNGT 5 cut(s) 7, 25, 32, 39, 1163
HpyCH4IV ACGT 2 cut(s) 357, 1044
HpyCH4V TGCA 2 cut(s) 264, 823
HpyF10VI GCNNNNNNNGC 7 cut(s) 69, 422, 647, 760, 825, 850, 1155
HpyF3I CTNAG 4 cut(s) 653, 672, 857, 869
HpySE526I ACGT 2 cut(s) 357, 1044
Hsp92I GRCGYC 2 cut(s) 357, 1133
Hsp92II CATG 3 cut(s) 268, 472, 1175
HspAI GCGC 7 cut(s) 93, 95, 498, 741, 763, 938, 1017
Ksp22I TGATCA 1 cut(s) 1074
LguI GCTCTTC 1 cut(s) 222
LmnI GCTCC 2 cut(s) 685, 1069
Lsp1109I GCAGC 3 cut(s) 222, 840, 927
LweI GCATC 1 cut(s) 1158
MaeI CTAG 1 cut(s) 239
MaeII ACGT 2 cut(s) 357, 1044
MaeIII GTNAC 2 cut(s) 7, 628
MhlI GDGCHC 3 cut(s) 652, 952, 983
MluCI AATT 4 cut(s) 113, 154, 163, 438
MluI ACGCGT 1 cut(s) 1111
MlyI GAGTC 2 cut(s) 572, 683
MmeI TCCRAC 2 cut(s) 405, 606
MroXI GAANNNNTTC 2 cut(s) 923, 1055
MseI TTAA 1 cut(s) 770
MslI CAYNNNNRTG 1 cut(s) 209
MspA1I CMGCKG 1 cut(s) 235
MspI CCGG 4 cut(s) 57, 251, 459, 776
MspR9I CCNGG 7 cut(s) 251, 353, 446, 482, 777, 953, 1085
MvaI CCWGG 5 cut(s) 353, 446, 482, 953, 1085
MwoI GCNNNNNNNGC 7 cut(s) 69, 422, 647, 760, 825, 850, 1155
NciI CCSGG 2 cut(s) 251, 777
NheI GCTAGC 1 cut(s) 238
NlaIII CATG 3 cut(s) 268, 472, 1175
NlaIV GGNNCC 2 cut(s) 512, 650
NmeAIII GCCGAG 2 cut(s) 358, 514
NmuCI GTSAC 1 cut(s) 628
NspI RCATGY 1 cut(s) 268
PaeR7I CTCGAG 1 cut(s) 560
PagI TCATGA 1 cut(s) 468
PauI GCGCGC 1 cut(s) 93
PciSI GCTCTTC 1 cut(s) 222
PcsI WCGNNNNNNNCGW 2 cut(s) 429, 1110
PdmI GAANNNNTTC 2 cut(s) 923, 1055
PfeI GAWTC 2 cut(s) 451, 690
PfoI TCCNGGA 1 cut(s) 444
PkrI GCNGC 6 cut(s) 65, 234, 237, 427, 855, 942
Ple19I CGATCG 3 cut(s) 612, 663, 1210
PleI GAGTC 2 cut(s) 572, 682
PpsI GAGTC 2 cut(s) 572, 682
Psp6I CCWGG 5 cut(s) 351, 444, 480, 951, 1083
PspGI CCWGG 5 cut(s) 351, 444, 480, 951, 1083
PspN4I GGNNCC 2 cut(s) 512, 650
PspOMI GGGCCC 1 cut(s) 648
PspPI GGNCC 7 cut(s) 253, 327, 478, 493, 640, 648, 649
PteI GCGCGC 1 cut(s) 93
PvuI CGATCG 3 cut(s) 612, 663, 1210
RsaI GTAC 6 cut(s) 126, 281, 302, 548, 848, 1040
RsaNI GTAC 6 cut(s) 125, 280, 301, 547, 847, 1039
RseI CAYNNNNRTG 1 cut(s) 209
Rsr2I CGGWCCG 1 cut(s) 253
RsrII CGGWCCG 1 cut(s) 253
SalI GTCGAC 1 cut(s) 341
SapI GCTCTTC 1 cut(s) 222
SaqAI TTAA 1 cut(s) 770
SatI GCNGC 6 cut(s) 64, 233, 236, 426, 854, 941
Sau96I GGNCC 7 cut(s) 253, 327, 478, 493, 640, 648, 649
SchI GAGTC 2 cut(s) 572, 683
ScrFI CCNGG 7 cut(s) 251, 353, 446, 482, 777, 953, 1085
SduI GDGCHC 3 cut(s) 652, 952, 983
SfaNI GCATC 1 cut(s) 1158
SfiI GGCCNNNNNGGCC 1 cut(s) 647
Sfr274I CTCGAG 1 cut(s) 560
SinI GGWCC 3 cut(s) 253, 327, 478
SlaI CTCGAG 1 cut(s) 560
SmiMI CAYNNNNRTG 1 cut(s) 209
SmlI CTYRAG 1 cut(s) 560
SmoI CTYRAG 1 cut(s) 560
Sse9I AATT 4 cut(s) 113, 154, 163, 438
SspMI CTAG 1 cut(s) 239
StyD4I CCNGG 7 cut(s) 249, 351, 444, 480, 775, 951, 1083
TaaI ACNGT 5 cut(s) 7, 25, 32, 39, 1163
TaiI ACGT 2 cut(s) 360, 1047
TasI AATT 4 cut(s) 113, 154, 163, 438
TatI WGTACW 4 cut(s) 300, 546, 846, 1038
TauI GCSGC 3 cut(s) 66, 235, 428
TfiI GAWTC 2 cut(s) 451, 690
Tru1I TTAA 1 cut(s) 770
Tru9I TTAA 1 cut(s) 770
TscAI CASTG 3 cut(s) 371, 650, 830
TseFI GTSAC 1 cut(s) 628
TseI GCWGC 3 cut(s) 235, 853, 940
Tsp45I GTSAC 1 cut(s) 628
TspDTI ATGAA 6 cut(s) 131, 190, 457, 485, 977, 1157
TspRI CASTG 3 cut(s) 371, 650, 830
VpaK11BI GGWCC 3 cut(s) 253, 327, 478
XagI CCTNNNNNAGG 1 cut(s) 350
XapI RAATTY 3 cut(s) 113, 154, 163
XceI RCATGY 1 cut(s) 268
XhoI CTCGAG 1 cut(s) 560
XmiI GTMKAC 1 cut(s) 342
XmnI GAANNNNTTC 2 cut(s) 923, 1055
XspI CTAG 1 cut(s) 239
ZraI GACGTC 1 cut(s) 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.