AT3G27340

Protein of unknown function (DUF971)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008779
Physical Location & Seq
Forward (+)
10121234 .. 10123128
1895 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G27340.1

Sequence Viewer

Length: 426 bp
ATGTGGGCGATTACGAGAGTGATAAGCCGGCGAGTTCATGGAACAAGTTTTATTACTATTCCAAAACTCTCAGGTTTCTCTATTGTATCTCCCAAACAAGTTGAGGTAGAGTATGCAGATGGAACCAAGTTCAATTTTTCATCAGAGTTTCTGAGAATTCATAGTCCAGCTGCTGATGGAAAAGTCAGATCAATCGGTGGTGAAAAGGTGATATCTGGAAGGCGGTATGTAGGAATCATGTCTGCAGAACCGGTTGGAAACTACGGGGTAAGGTTAGTGTTTGATGACTTGCATAGAACAGGGATATATCCATGGGATTATTTCTACGAGCTTGGGAGCAATAAGTTTGGTCTTATGAGGAACTATATCAAGACTCTTCAAAAGCATAATCTCAGCCGTGAACCTCCTCCTTCAAGAAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF971

Protein Analysis

141

Amino Acids

16.08

Weight (kDa)

10.09

Isoelectric Point (pI)

50.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GBBH-like_N PF06155 32 - 109 3.8e-20 Gamma-butyrobetaine hydroxylase-like, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 223
AcsI RAATTY 1 cut(s) 156
AgeI ACCGGT 1 cut(s) 250
AgsI TTSAA 3 cut(s) 133, 380, 414
AluBI AGCT 2 cut(s) 170, 331
AluI AGCT 2 cut(s) 170, 331
AlwNI CAGNNNCTG 1 cut(s) 173
ApeKI GCWGC 1 cut(s) 170
ApoI RAATTY 1 cut(s) 156
AsiGI ACCGGT 1 cut(s) 250
AsuHPI GGTGA 2 cut(s) 212, 220
BbvI GCAGC 1 cut(s) 157
BccI CCATC 2 cut(s) 113, 170
BceAI ACGGC 1 cut(s) 381
BfmI CTRYAG 1 cut(s) 243
BisI GCNGC 1 cut(s) 171
BlsI GCNGC 1 cut(s) 172
BmiI GGNNCC 1 cut(s) 124
BsaJI CCNNGG 1 cut(s) 311
BsaWI WCCGGW 1 cut(s) 250
Bse118I RCCGGY 2 cut(s) 27, 250
BseDI CCNNGG 1 cut(s) 311
BseMII CTCAG 3 cut(s) 84, 143, 406
BseRI GAGGAG 1 cut(s) 396
BseXI GCAGC 1 cut(s) 157
BshTI ACCGGT 1 cut(s) 250
BsiSI CCGG 2 cut(s) 28, 251
Bsp143I GATC 1 cut(s) 188
Bsp19I CCATGG 1 cut(s) 311
BspACI CCGC 1 cut(s) 223
BspCNI CTCAG 3 cut(s) 83, 144, 405
BspLI GGNNCC 1 cut(s) 124
BspMAI CTGCAG 1 cut(s) 247
BsrFI RCCGGY 2 cut(s) 27, 250
BssAI RCCGGY 2 cut(s) 27, 250
BssECI CCNNGG 1 cut(s) 311
BssMI GATC 1 cut(s) 188
BssT1I CCWWGG 1 cut(s) 311
Bst6I CTCTTC 1 cut(s) 381
BstC8I GCNNGC 1 cut(s) 29
BstDEI CTNAG 3 cut(s) 70, 152, 392
BstDSI CCRYGG 1 cut(s) 311
BstKTI GATC 1 cut(s) 191
BstMBI GATC 1 cut(s) 188
BstSFI CTRYAG 1 cut(s) 243
BstV1I GCAGC 1 cut(s) 157
BtgI CCRYGG 1 cut(s) 311
Cac8I GCNNGC 1 cut(s) 29
CaiI CAGNNNCTG 1 cut(s) 173
Cfr10I RCCGGY 2 cut(s) 27, 250
CspAI ACCGGT 1 cut(s) 250
CviAII CATG 3 cut(s) 38, 238, 312
CviJI RGCY 4 cut(s) 27, 170, 331, 396
CviKI_1 RGCY 4 cut(s) 27, 170, 331, 396
DdeI CTNAG 3 cut(s) 70, 152, 392
DpnI GATC 1 cut(s) 190
DpnII GATC 1 cut(s) 188
Eam1104I CTCTTC 1 cut(s) 381
EarI CTCTTC 1 cut(s) 381
Eco130I CCWWGG 1 cut(s) 311
Eco32I GATATC 1 cut(s) 213
EcoRI GAATTC 1 cut(s) 156
EcoRV GATATC 1 cut(s) 213
EcoT14I CCWWGG 1 cut(s) 311
ErhI CCWWGG 1 cut(s) 311
FaeI CATG 3 cut(s) 41, 241, 315
FatI CATG 3 cut(s) 37, 237, 311
Fnu4HI GCNGC 1 cut(s) 171
Fsp4HI GCNGC 1 cut(s) 171
GluI GCNGC 1 cut(s) 171
HapII CCGG 2 cut(s) 28, 251
Hin1II CATG 3 cut(s) 41, 241, 315
HinfI GANTC 2 cut(s) 234, 373
HpaII CCGG 2 cut(s) 28, 251
HphI GGTGA 2 cut(s) 212, 220
Hpy166II GTNNAC 1 cut(s) 401
Hpy188I TCNGA 3 cut(s) 145, 153, 188
Hpy188III TCNNGA 3 cut(s) 216, 370, 414
Hpy8I GTNNAC 1 cut(s) 401
HpyAV CCTTC 2 cut(s) 213, 420
HpyCH4V TGCA 3 cut(s) 116, 245, 292
HpyF3I CTNAG 3 cut(s) 70, 152, 392
Hsp92II CATG 3 cut(s) 41, 241, 315
KroI GCCGGC 1 cut(s) 27
KroNI GCCGGC 1 cut(s) 29
Kzo9I GATC 1 cut(s) 188
LmnI GCTCC 1 cut(s) 336
LpnPI CCDG 6 cut(s) 41, 57, 180, 201, 264, 285
Lsp1109I GCAGC 1 cut(s) 157
MalI GATC 1 cut(s) 190
MboI GATC 1 cut(s) 188
MboII GAAGA 1 cut(s) 368
MluCI AATT 2 cut(s) 133, 156
MlyI GAGTC 1 cut(s) 367
MmeI TCCRAC 1 cut(s) 235
MnlI CCTC 4 cut(s) 97, 351, 414, 417
MroNI GCCGGC 1 cut(s) 27
MspA1I CMGCKG 1 cut(s) 170
MspI CCGG 2 cut(s) 28, 251
NaeI GCCGGC 1 cut(s) 29
NcoI CCATGG 1 cut(s) 311
NdeII GATC 1 cut(s) 188
NgoMIV GCCGGC 1 cut(s) 27
NlaIII CATG 3 cut(s) 41, 241, 315
NlaIV GGNNCC 1 cut(s) 124
PdiI GCCGGC 1 cut(s) 29
PfeI GAWTC 1 cut(s) 234
PinAI ACCGGT 1 cut(s) 250
PkrI GCNGC 1 cut(s) 172
PleI GAGTC 1 cut(s) 367
PpsI GAGTC 1 cut(s) 367
PspN4I GGNNCC 1 cut(s) 124
PstI CTGCAG 1 cut(s) 247
PstNI CAGNNNCTG 1 cut(s) 173
PvuII CAGCTG 1 cut(s) 170
SatI GCNGC 1 cut(s) 171
Sau3AI GATC 1 cut(s) 188
SchI GAGTC 1 cut(s) 367
SetI ASST 7 cut(s) 76, 108, 172, 210, 275, 333, 406
SfcI CTRYAG 1 cut(s) 243
Sse9I AATT 2 cut(s) 133, 156
SsiI CCGC 1 cut(s) 223
StyI CCWWGG 1 cut(s) 311
TasI AATT 2 cut(s) 133, 156
TfiI GAWTC 1 cut(s) 234
TseI GCWGC 1 cut(s) 170
TspDTI ATGAA 3 cut(s) 26, 129, 149
XapI RAATTY 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.