Rh2AG653800

Protein of unknown function (DUF971)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0008779
Physical Location & Seq
Reverse (-)
87144583 .. 87145828
1246 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG653800.1

Sequence Viewer

Length: 411 bp
ATGCTCGCCCTCCACAGAGCGGTCCGGCGGTTCCACGCTACCGCCGATGCTCCGCCGCTCACCAGATTCGCCCTCCACGCGCCTAAATATGTAGAGGTGGAGTTTGGCAATGGCAGTGTGTTCAACTTGTCAGCTGAGTTCTTAAGAGTTCACAGTCCAGCTGTTGACGCCAAGATTAGGTCCATTGCTGGTGAAAAGGTGATATCGGGGCGGCGTCTGGTGGGGATCATGTCTGCAGAACCTGTAGGAAACTATGGGGTGAGGATTCTTTTCGATGACTTGCATAAGACTGGCATATATTCGTGGGATTACTTTTATCATTTGGGGAGTAACAAGTTTAGCCTCATGAGAAGTTATATCAAGACTCTCAACAAGTATGGACTCCGGCGAGATCCGCCTAGAAGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF971

Protein Analysis

136

Amino Acids

15.49

Weight (kDa)

10.27

Isoelectric Point (pI)

51.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GBBH-like_N PF06155 29 - 106 1.6e-18 Gamma-butyrobetaine hydroxylase-like, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 20, 58
AccII CGCG 1 cut(s) 80
AciI CCGC 7 cut(s) 20, 28, 42, 53, 56, 211, 395
AclWI GGATC 2 cut(s) 233, 386
AcyI GRCGYC 2 cut(s) 168, 214
AfiI CCNNNNNNNGG 2 cut(s) 19, 177
AflII CTTAAG 1 cut(s) 142
AgsI TTSAA 1 cut(s) 124
AluBI AGCT 2 cut(s) 134, 161
AluI AGCT 2 cut(s) 134, 161
AlwI GGATC 2 cut(s) 233, 386
AlwNI CAGNNNCTG 1 cut(s) 242
AspLEI GCGC 1 cut(s) 82
AspS9I GGNCC 2 cut(s) 22, 180
AsuHPI GGTGA 4 cut(s) 52, 203, 211, 271
AvaII GGWCC 2 cut(s) 22, 180
BfaI CTAG 1 cut(s) 399
BfmI CTRYAG 2 cut(s) 234, 243
BfrI CTTAAG 1 cut(s) 142
BisI GCNGC 2 cut(s) 56, 212
BlsI GCNGC 2 cut(s) 57, 213
Bme18I GGWCC 2 cut(s) 22, 180
BmgT120I GGNCC 2 cut(s) 22, 180
BmiI GGNNCC 1 cut(s) 32
BmsI GCATC 1 cut(s) 37
BsaHI GRCGYC 2 cut(s) 168, 214
Bsc4I CCNNNNNNNGG 2 cut(s) 19, 177
Bse1I ACTGG 1 cut(s) 295
Bse3DI GCAATG 2 cut(s) 115, 183
BseLI CCNNNNNNNGG 2 cut(s) 19, 177
BseMI GCAATG 2 cut(s) 115, 183
BseMII CTCAG 1 cut(s) 126
BseNI ACTGG 1 cut(s) 295
Bsh1236I CGCG 1 cut(s) 80
BsiSI CCGG 2 cut(s) 25, 385
BslI CCNNNNNNNGG 2 cut(s) 19, 177
Bsp143I GATC 2 cut(s) 225, 391
BspACI CCGC 7 cut(s) 20, 28, 42, 53, 56, 211, 395
BspCNI CTCAG 1 cut(s) 127
BspFNI CGCG 1 cut(s) 80
BspHI TCATGA 1 cut(s) 345
BspLI GGNNCC 1 cut(s) 32
BspMAI CTGCAG 1 cut(s) 238
BspPI GGATC 2 cut(s) 233, 386
BspTI CTTAAG 1 cut(s) 142
BsrBI CCGCTC 2 cut(s) 20, 58
BsrDI GCAATG 2 cut(s) 115, 183
BsrI ACTGG 1 cut(s) 295
BssMI GATC 2 cut(s) 225, 391
BssNI GRCGYC 2 cut(s) 168, 214
Bst4CI ACNGT 1 cut(s) 155
BstACI GRCGYC 2 cut(s) 168, 214
BstAFI CTTAAG 1 cut(s) 142
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 1 cut(s) 135
BstFNI CGCG 1 cut(s) 80
BstHHI GCGC 1 cut(s) 82
BstKTI GATC 2 cut(s) 228, 394
BstMBI GATC 2 cut(s) 225, 391
BstMWI GCNNNNNNNGC 3 cut(s) 77, 167, 394
BstSFI CTRYAG 2 cut(s) 234, 243
BstUI CGCG 1 cut(s) 80
BstX2I RGATCY 1 cut(s) 391
BstYI RGATCY 1 cut(s) 391
BtsI GCAGTG 1 cut(s) 121
BtsIMutI CAGTG 1 cut(s) 121
Cac8I GCNNGC 1 cut(s) 6
CaiI CAGNNNCTG 1 cut(s) 242
CciI TCATGA 1 cut(s) 345
CfoI GCGC 1 cut(s) 82
Cfr13I GGNCC 2 cut(s) 22, 180
CpoI CGGWCCG 1 cut(s) 22
CseI GACGC 2 cut(s) 176, 203
CspI CGGWCCG 1 cut(s) 22
CviAII CATG 2 cut(s) 229, 346
CviJI RGCY 3 cut(s) 134, 161, 342
CviKI_1 RGCY 3 cut(s) 134, 161, 342
DdeI CTNAG 1 cut(s) 135
DpnI GATC 2 cut(s) 227, 393
DpnII GATC 2 cut(s) 225, 391
EciI GGCGGA 2 cut(s) 42, 384
Eco32I GATATC 1 cut(s) 204
Eco47I GGWCC 2 cut(s) 22, 180
EcoRV GATATC 1 cut(s) 204
FaeI CATG 2 cut(s) 232, 349
FaiI YATR 9 cut(s) 90, 230, 255, 285, 296, 298, 347, 357, 378
FatI CATG 2 cut(s) 228, 345
Fnu4HI GCNGC 2 cut(s) 56, 212
Fsp4HI GCNGC 2 cut(s) 56, 212
FspBI CTAG 1 cut(s) 399
GlaI GCGC 1 cut(s) 81
GluI GCNGC 2 cut(s) 56, 212
HapII CCGG 2 cut(s) 25, 385
HgaI GACGC 2 cut(s) 176, 203
HhaI GCGC 1 cut(s) 82
Hin1I GRCGYC 2 cut(s) 168, 214
Hin1II CATG 2 cut(s) 232, 349
Hin6I GCGC 1 cut(s) 80
HinP1I GCGC 1 cut(s) 80
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HinfI GANTC 4 cut(s) 66, 265, 364, 381
HpaII CCGG 2 cut(s) 25, 385
HphI GGTGA 4 cut(s) 52, 203, 211, 271
Hpy166II GTNNAC 2 cut(s) 151, 166
Hpy188III TCNNGA 2 cut(s) 346, 361
Hpy8I GTNNAC 2 cut(s) 151, 166
HpyAV CCTTC 1 cut(s) 396
HpyCH4III ACNGT 1 cut(s) 155
HpyCH4V TGCA 2 cut(s) 236, 283
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 167, 394
HpyF3I CTNAG 1 cut(s) 135
Hsp92I GRCGYC 2 cut(s) 168, 214
Hsp92II CATG 2 cut(s) 232, 349
HspAI GCGC 1 cut(s) 80
Kzo9I GATC 2 cut(s) 225, 391
LmnI GCTCC 1 cut(s) 55
LpnPI CCDG 8 cut(s) 38, 76, 171, 174, 203, 255, 276, 398
LweI GCATC 1 cut(s) 37
MaeI CTAG 1 cut(s) 399
MaeIII GTNAC 1 cut(s) 329
MalI GATC 2 cut(s) 227, 393
MbiI CCGCTC 2 cut(s) 20, 58
MboI GATC 2 cut(s) 225, 391
MflI RGATCY 1 cut(s) 391
MlyI GAGTC 2 cut(s) 358, 375
MnlI CCTC 5 cut(s) 20, 83, 88, 255, 353
MseI TTAA 1 cut(s) 143
MspA1I CMGCKG 2 cut(s) 134, 161
MspCI CTTAAG 1 cut(s) 142
MspI CCGG 2 cut(s) 25, 385
MvnI CGCG 1 cut(s) 80
MwoI GCNNNNNNNGC 3 cut(s) 77, 167, 394
NdeII GATC 2 cut(s) 225, 391
NlaIII CATG 2 cut(s) 232, 349
NlaIV GGNNCC 1 cut(s) 32
PagI TCATGA 1 cut(s) 345
PcsI WCGNNNNNNNCGW 1 cut(s) 42
PfeI GAWTC 2 cut(s) 66, 265
PkrI GCNGC 2 cut(s) 57, 213
PleI GAGTC 2 cut(s) 358, 375
PpsI GAGTC 2 cut(s) 358, 375
PspN4I GGNNCC 1 cut(s) 32
PspPI GGNCC 2 cut(s) 22, 180
PstI CTGCAG 1 cut(s) 238
PstNI CAGNNNCTG 1 cut(s) 242
PsuI RGATCY 1 cut(s) 391
PvuII CAGCTG 2 cut(s) 134, 161
Rsr2I CGGWCCG 1 cut(s) 22
RsrII CGGWCCG 1 cut(s) 22
SaqAI TTAA 1 cut(s) 143
SatI GCNGC 2 cut(s) 56, 212
Sau3AI GATC 2 cut(s) 225, 391
Sau96I GGNCC 2 cut(s) 22, 180
SchI GAGTC 2 cut(s) 358, 375
SetI ASST 6 cut(s) 99, 136, 163, 182, 201, 244
SfaNI GCATC 1 cut(s) 37
SfcI CTRYAG 2 cut(s) 234, 243
SinI GGWCC 2 cut(s) 22, 180
SmlI CTYRAG 1 cut(s) 142
SmoI CTYRAG 1 cut(s) 142
SsiI CCGC 7 cut(s) 20, 28, 42, 53, 56, 211, 395
SspMI CTAG 1 cut(s) 399
TaaI ACNGT 1 cut(s) 155
TaqI TCGA 1 cut(s) 273
TauI GCSGC 2 cut(s) 58, 214
TfiI GAWTC 2 cut(s) 66, 265
Tru1I TTAA 1 cut(s) 143
Tru9I TTAA 1 cut(s) 143
TscAI CASTG 1 cut(s) 121
TspRI CASTG 1 cut(s) 121
Vha464I CTTAAG 1 cut(s) 142
VpaK11BI GGWCC 2 cut(s) 22, 180
XspI CTAG 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.