Rroxscaffold_2G00078480

Protein of unknown function (DUF971)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008779
Physical Location & Seq
Forward (+)
1770783 .. 1771857
1075 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00078480.1

Sequence Viewer

Length: 468 bp
ATGCTTGCCCTCCACAGAGCGGTCCGGCGGTTCCACGCTACCGCCGATGCTCCGCCGCTCACCAGATTCGCCCTCCACGCTCCTAAATATGTAGAGGTGGAGTTTGGCAATGGGAGTGTGTTCAACTTGTCAGCTGAGTTCTTAAGAGTTCACAGTCCAGCTGTCGATGCCAAGATTAGGTCCATTGCTGGTGAAAAGGTGATATCGGGGCGGCGTCTGGTGGGGATCATGTCTGCAGAACCTGTAGGAAACTATGGGGTGAGGATTCTTTTCGATGACCTGCATAGGACTGGCATCTATTCGTGGGATTACTTGTATCATTTGGGGAGTAACAAGTTTAGCCTCATGAGAAGTTATATCAAGACTCTCAACAACCTTTTATCTATAATTGAAGCAGCATTGGTGGAGGATGATGCAGCTTGTTTTAGTTTCGAATTCATTGCTTGGAGTCTTAGAGCTAAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF971

Protein Analysis

155

Amino Acids

17.42

Weight (kDa)

8.76

Isoelectric Point (pI)

40.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GBBH-like_N PF06155 29 - 106 4.7e-19 Gamma-butyrobetaine hydroxylase-like, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 288
AccBSI CCGCTC 2 cut(s) 20, 58
AciI CCGC 6 cut(s) 20, 28, 42, 53, 56, 211
AclWI GGATC 1 cut(s) 233
AcsI RAATTY 1 cut(s) 434
AcyI GRCGYC 1 cut(s) 214
AfiI CCNNNNNNNGG 2 cut(s) 19, 177
AflII CTTAAG 1 cut(s) 142
AgsI TTSAA 2 cut(s) 124, 392
AluBI AGCT 4 cut(s) 134, 161, 419, 458
AluI AGCT 4 cut(s) 134, 161, 419, 458
AlwI GGATC 1 cut(s) 233
AlwNI CAGNNNCTG 1 cut(s) 242
ApeKI GCWGC 2 cut(s) 395, 416
ApoI RAATTY 1 cut(s) 434
AspS9I GGNCC 2 cut(s) 22, 180
AsuHPI GGTGA 4 cut(s) 52, 203, 211, 271
AsuII TTCGAA 1 cut(s) 432
AvaII GGWCC 2 cut(s) 22, 180
BbvI GCAGC 2 cut(s) 407, 428
BcgI CGANNNNNNTGC 2 cut(s) 422, 456
BfmI CTRYAG 2 cut(s) 234, 243
BfrI CTTAAG 1 cut(s) 142
BfuAI ACCTGC 1 cut(s) 288
BisI GCNGC 4 cut(s) 56, 212, 396, 417
BlsI GCNGC 4 cut(s) 57, 213, 397, 418
Bme18I GGWCC 2 cut(s) 22, 180
BmgT120I GGNCC 2 cut(s) 22, 180
BmiI GGNNCC 1 cut(s) 32
BmsI GCATC 4 cut(s) 37, 157, 303, 403
Bpu14I TTCGAA 1 cut(s) 432
BsaHI GRCGYC 1 cut(s) 214
Bsc4I CCNNNNNNNGG 2 cut(s) 19, 177
Bse1I ACTGG 1 cut(s) 295
Bse3DI GCAATG 3 cut(s) 115, 183, 438
BseGI GGATG 1 cut(s) 415
BseLI CCNNNNNNNGG 2 cut(s) 19, 177
BseMI GCAATG 3 cut(s) 115, 183, 438
BseMII CTCAG 1 cut(s) 126
BseNI ACTGG 1 cut(s) 295
BseXI GCAGC 2 cut(s) 407, 428
BsiSI CCGG 1 cut(s) 25
BslI CCNNNNNNNGG 2 cut(s) 19, 177
Bsp119I TTCGAA 1 cut(s) 432
Bsp143I GATC 1 cut(s) 225
BspACI CCGC 6 cut(s) 20, 28, 42, 53, 56, 211
BspCNI CTCAG 1 cut(s) 127
BspHI TCATGA 1 cut(s) 345
BspLI GGNNCC 1 cut(s) 32
BspMAI CTGCAG 1 cut(s) 238
BspMI ACCTGC 1 cut(s) 288
BspPI GGATC 1 cut(s) 233
BspT104I TTCGAA 1 cut(s) 432
BspTI CTTAAG 1 cut(s) 142
BsrBI CCGCTC 2 cut(s) 20, 58
BsrDI GCAATG 3 cut(s) 115, 183, 438
BsrI ACTGG 1 cut(s) 295
BssMI GATC 1 cut(s) 225
BssNI GRCGYC 1 cut(s) 214
Bst4CI ACNGT 1 cut(s) 155
BstACI GRCGYC 1 cut(s) 214
BstAFI CTTAAG 1 cut(s) 142
BstBI TTCGAA 1 cut(s) 432
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 2 cut(s) 135, 452
BstF5I GGATG 1 cut(s) 415
BstKTI GATC 1 cut(s) 228
BstMBI GATC 1 cut(s) 225
BstMWI GCNNNNNNNGC 2 cut(s) 77, 167
BstSFI CTRYAG 2 cut(s) 234, 243
BstV1I GCAGC 2 cut(s) 407, 428
BtsCI GGATG 1 cut(s) 415
BveI ACCTGC 1 cut(s) 288
Cac8I GCNNGC 1 cut(s) 6
CaiI CAGNNNCTG 1 cut(s) 242
CciI TCATGA 1 cut(s) 345
Cfr13I GGNCC 2 cut(s) 22, 180
CpoI CGGWCCG 1 cut(s) 22
CseI GACGC 1 cut(s) 203
CspI CGGWCCG 1 cut(s) 22
CviAII CATG 2 cut(s) 229, 346
CviJI RGCY 5 cut(s) 134, 161, 342, 419, 458
CviKI_1 RGCY 5 cut(s) 134, 161, 342, 419, 458
DdeI CTNAG 2 cut(s) 135, 452
DpnI GATC 1 cut(s) 227
DpnII GATC 1 cut(s) 225
EciI GGCGGA 1 cut(s) 42
Eco32I GATATC 1 cut(s) 204
Eco47I GGWCC 2 cut(s) 22, 180
EcoRI GAATTC 1 cut(s) 434
EcoRV GATATC 1 cut(s) 204
FaeI CATG 2 cut(s) 232, 349
FaiI YATR 7 cut(s) 90, 230, 255, 285, 347, 357, 386
FatI CATG 2 cut(s) 228, 345
Fnu4HI GCNGC 4 cut(s) 56, 212, 396, 417
FokI GGATG 1 cut(s) 422
Fsp4HI GCNGC 4 cut(s) 56, 212, 396, 417
GluI GCNGC 4 cut(s) 56, 212, 396, 417
HapII CCGG 1 cut(s) 25
HgaI GACGC 1 cut(s) 203
Hin1I GRCGYC 1 cut(s) 214
Hin1II CATG 2 cut(s) 232, 349
HinfI GANTC 4 cut(s) 66, 265, 364, 448
HpaII CCGG 1 cut(s) 25
HphI GGTGA 4 cut(s) 52, 203, 211, 271
Hpy166II GTNNAC 1 cut(s) 151
Hpy188III TCNNGA 2 cut(s) 346, 361
Hpy8I GTNNAC 1 cut(s) 151
HpyCH4III ACNGT 1 cut(s) 155
HpyCH4V TGCA 3 cut(s) 236, 283, 416
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 167
HpyF3I CTNAG 2 cut(s) 135, 452
Hsp92I GRCGYC 1 cut(s) 214
Hsp92II CATG 2 cut(s) 232, 349
Kzo9I GATC 1 cut(s) 225
LmnI GCTCC 2 cut(s) 55, 85
LpnPI CCDG 8 cut(s) 38, 76, 171, 174, 203, 255, 276, 293
Lsp1109I GCAGC 2 cut(s) 407, 428
LweI GCATC 4 cut(s) 37, 157, 303, 403
MaeIII GTNAC 1 cut(s) 329
MalI GATC 1 cut(s) 227
MbiI CCGCTC 2 cut(s) 20, 58
MboI GATC 1 cut(s) 225
MluCI AATT 2 cut(s) 387, 434
MlyI GAGTC 2 cut(s) 358, 457
MnlI CCTC 6 cut(s) 20, 83, 88, 255, 353, 400
MseI TTAA 1 cut(s) 143
MspA1I CMGCKG 2 cut(s) 134, 161
MspCI CTTAAG 1 cut(s) 142
MspI CCGG 1 cut(s) 25
MwoI GCNNNNNNNGC 2 cut(s) 77, 167
NdeII GATC 1 cut(s) 225
NlaIII CATG 2 cut(s) 232, 349
NlaIV GGNNCC 1 cut(s) 32
NspV TTCGAA 1 cut(s) 432
PagI TCATGA 1 cut(s) 345
PcsI WCGNNNNNNNCGW 1 cut(s) 42
PfeI GAWTC 2 cut(s) 66, 265
PkrI GCNGC 4 cut(s) 57, 213, 397, 418
PleI GAGTC 2 cut(s) 358, 456
PpsI GAGTC 2 cut(s) 358, 456
PspN4I GGNNCC 1 cut(s) 32
PspPI GGNCC 2 cut(s) 22, 180
PstI CTGCAG 1 cut(s) 238
PstNI CAGNNNCTG 1 cut(s) 242
PvuII CAGCTG 2 cut(s) 134, 161
Rsr2I CGGWCCG 1 cut(s) 22
RsrII CGGWCCG 1 cut(s) 22
SaqAI TTAA 1 cut(s) 143
SatI GCNGC 4 cut(s) 56, 212, 396, 417
Sau3AI GATC 1 cut(s) 225
Sau96I GGNCC 2 cut(s) 22, 180
SchI GAGTC 2 cut(s) 358, 457
SfaNI GCATC 4 cut(s) 37, 157, 303, 403
SfcI CTRYAG 2 cut(s) 234, 243
SfuI TTCGAA 1 cut(s) 432
SinI GGWCC 2 cut(s) 22, 180
SmlI CTYRAG 1 cut(s) 142
SmoI CTYRAG 1 cut(s) 142
Sse9I AATT 2 cut(s) 387, 434
SsiI CCGC 6 cut(s) 20, 28, 42, 53, 56, 211
TaaI ACNGT 1 cut(s) 155
TaqI TCGA 3 cut(s) 165, 273, 432
TasI AATT 2 cut(s) 387, 434
TauI GCSGC 2 cut(s) 58, 214
TfiI GAWTC 2 cut(s) 66, 265
Tru1I TTAA 1 cut(s) 143
Tru9I TTAA 1 cut(s) 143
TseI GCWGC 2 cut(s) 395, 416
TspDTI ATGAA 1 cut(s) 427
Vha464I CTTAAG 1 cut(s) 142
VpaK11BI GGWCC 2 cut(s) 22, 180
XapI RAATTY 1 cut(s) 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.