AT3G27650

lob domain-containing protein 25

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0013925
Physical Location & Seq
Forward (+)
10238514 .. 10240648
2135 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G27650.1

Sequence Viewer

Length: 480 bp
ATGCCCAAGAGAGAAACAAAGAAGATCAAACCTTCTCAAGAAGTTATCAAGGAAGGACCTTTTCTTGTTGCGATCCATTTAAAGGGGATATATATGTCGAACTACACAAACTCACCGTGTGCAGCATGCAAATTCCTCCGGCGTAAATGCACGTCAGACTGCGTATTCGCACCCTATTTTCCGCCGGAGGAACCTACAAAGTTTGCGAACGTCCACCGGATATTCGGGGCAAGCAATGTGAGCAAGATCCTCCACGAAGTGGCTCCCCATCAGCGGGAAGATGCGGTCAACTCGCTGGCTTACGAGGCGGAAGCACGACTTAAAGATCCAGTGTATGGCTGCGTTGGAGCGATCTCGGTGCTCCAAAGACAGGTCTTGAGGTTGCAAAGGGAACTCGAGGAGACAAATGCTGATCTCATGAGGTACGCTGGTTGTCTCGGTGGTGAAACGACGTCGGCTTATGGTGGTCGGAGGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

159

Amino Acids

17.75

Weight (kDa)

9.05

Isoelectric Point (pI)

52.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 39 - 136 5.5e-42 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013925)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27650
fragaria_vesca FvH4_6g51300 FvH4_6g51300 FvH4_6g51300
malus_domestica MD17G1029300.v1.1
prunus_persica Prupe.3G290800_v2.0.a1
pyrus_communis pycom17g02650
rosa_chinensis RchiOBHm_Chr2g0172921
rosa_laevigata RLG00000022142
rosa_multiflora Rmu_sc0009727.1_g000002
rosa_roxburghii Rroxscaffold_2G00079420
rosa_rugosa Rorug02G0563600
rosa_samantha Rh2AG640400 Rh2BG653900 Rh2CG619500 Rh2DG667500
rosa_wichuraiana Rw2G052860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 455
AccB7I CCANNNNNTGG 2 cut(s) 259, 335
AciI CCGC 4 cut(s) 182, 274, 284, 308
AclWI GGATC 3 cut(s) 67, 241, 320
AcsI RAATTY 1 cut(s) 131
AcyI GRCGYC 1 cut(s) 452
AdeI CACNNNGTG 2 cut(s) 119, 259
AfaI GTAC 1 cut(s) 425
AfiI CCNNNNNNNGG 6 cut(s) 82, 259, 273, 274, 335, 370
AjiI CACGTC 1 cut(s) 153
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 2 cut(s) 395, 440
AlwI GGATC 3 cut(s) 67, 241, 320
Ama87I CYCGRG 1 cut(s) 395
ApeKI GCWGC 2 cut(s) 122, 339
ApoI RAATTY 1 cut(s) 131
ArsI GACNNNNNNTTYG 2 cut(s) 149, 181
AspS9I GGNCC 1 cut(s) 56
AsuHPI GGTGA 2 cut(s) 105, 455
AvaI CYCGRG 1 cut(s) 395
AvaII GGWCC 1 cut(s) 56
BaeI ACNNNNGTAYC 2 cut(s) 415, 448
Bbv12I GWGCWC 1 cut(s) 363
BbvI GCAGC 2 cut(s) 134, 326
BccI CCATC 1 cut(s) 276
BcgI CGANNNNNNTGC 2 cut(s) 340, 374
BcoDI GTCTC 2 cut(s) 395, 440
BisI GCNGC 2 cut(s) 123, 340
BlsI GCNGC 2 cut(s) 124, 341
Bme18I GGWCC 1 cut(s) 56
BmeT110I CYCGRG 1 cut(s) 395
BmgBI CACGTC 1 cut(s) 153
BmgT120I GGNCC 1 cut(s) 56
BmiI GGNNCC 2 cut(s) 192, 264
BmsI GCATC 1 cut(s) 271
BpuEI CTTGAG 2 cut(s) 21, 397
BsaHI GRCGYC 1 cut(s) 452
BsaWI WCCGGW 1 cut(s) 216
Bsc4I CCNNNNNNNGG 6 cut(s) 82, 259, 273, 274, 335, 370
Bse1I ACTGG 1 cut(s) 329
Bse3DI GCAATG 1 cut(s) 241
BseLI CCNNNNNNNGG 6 cut(s) 82, 259, 273, 274, 335, 370
BseMI GCAATG 1 cut(s) 241
BseNI ACTGG 1 cut(s) 329
BseRI GAGGAG 1 cut(s) 413
BseXI GCAGC 2 cut(s) 134, 326
BsgI GTGCAG 1 cut(s) 141
BsiHKAI GWGCWC 1 cut(s) 363
BsiHKCI CYCGRG 1 cut(s) 395
BsiSI CCGG 3 cut(s) 139, 185, 217
BslI CCNNNNNNNGG 6 cut(s) 82, 259, 273, 274, 335, 370
BsmAI GTCTC 2 cut(s) 395, 440
BsoBI CYCGRG 1 cut(s) 395
Bsp1286I GDGCHC 1 cut(s) 363
Bsp143I GATC 6 cut(s) 24, 72, 246, 325, 351, 412
BspACI CCGC 4 cut(s) 182, 274, 284, 308
BspHI TCATGA 1 cut(s) 417
BspLI GGNNCC 2 cut(s) 192, 264
BspPI GGATC 3 cut(s) 67, 241, 320
BsrDI GCAATG 1 cut(s) 241
BsrI ACTGG 1 cut(s) 329
BssMI GATC 6 cut(s) 24, 72, 246, 325, 351, 412
BssNI GRCGYC 1 cut(s) 452
Bst4CI ACNGT 1 cut(s) 117
BstACI GRCGYC 1 cut(s) 452
BstC8I GCNNGC 3 cut(s) 127, 232, 297
BstKTI GATC 6 cut(s) 27, 75, 249, 328, 354, 415
BstMAI GTCTC 2 cut(s) 395, 440
BstMBI GATC 6 cut(s) 24, 72, 246, 325, 351, 412
BstMWI GCNNNNNNNGC 2 cut(s) 240, 305
BstNSI RCATGY 1 cut(s) 129
BstV1I GCAGC 2 cut(s) 134, 326
BstX2I RGATCY 2 cut(s) 246, 325
BstYI RGATCY 2 cut(s) 246, 325
BtrI CACGTC 1 cut(s) 153
BtsIMutI CAGTG 1 cut(s) 336
Cac8I GCNNGC 3 cut(s) 127, 232, 297
CciI TCATGA 1 cut(s) 417
Cfr13I GGNCC 1 cut(s) 56
Csp6I GTAC 1 cut(s) 424
CviAII CATG 2 cut(s) 126, 418
CviJI RGCY 4 cut(s) 263, 299, 339, 458
CviKI_1 RGCY 4 cut(s) 263, 299, 339, 458
CviQI GTAC 1 cut(s) 424
DpnI GATC 6 cut(s) 26, 74, 248, 327, 353, 414
DpnII GATC 6 cut(s) 24, 72, 246, 325, 351, 412
DraI TTTAAA 1 cut(s) 81
DraIII CACNNNGTG 2 cut(s) 119, 259
EciI GGCGGA 2 cut(s) 171, 323
Eco47I GGWCC 1 cut(s) 56
Eco88I CYCGRG 1 cut(s) 395
EcoO109I RGGNCCY 1 cut(s) 56
FaeI CATG 2 cut(s) 129, 421
FaiI YATR 7 cut(s) 91, 93, 95, 127, 336, 419, 462
FalI AAGNNNNNCTT 2 cut(s) 303, 335
FatI CATG 2 cut(s) 125, 417
FauI CCCGC 1 cut(s) 267
Fnu4HI GCNGC 2 cut(s) 123, 340
Fsp4HI GCNGC 2 cut(s) 123, 340
GluI GCNGC 2 cut(s) 123, 340
HapII CCGG 3 cut(s) 139, 185, 217
Hin1I GRCGYC 1 cut(s) 452
Hin1II CATG 2 cut(s) 129, 421
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HpaII CCGG 3 cut(s) 139, 185, 217
HphI GGTGA 2 cut(s) 105, 455
Hpy166II GTNNAC 2 cut(s) 214, 289
Hpy188I TCNGA 2 cut(s) 157, 471
Hpy188III TCNNGA 3 cut(s) 38, 376, 418
Hpy8I GTNNAC 2 cut(s) 214, 289
Hpy99I CGWCG 2 cut(s) 454, 457
HpyAV CCTTC 2 cut(s) 42, 47
HpyCH4III ACNGT 1 cut(s) 117
HpyCH4IV ACGT 3 cut(s) 152, 210, 452
HpyCH4V TGCA 4 cut(s) 122, 129, 150, 385
HpyF10VI GCNNNNNNNGC 2 cut(s) 240, 305
HpySE526I ACGT 3 cut(s) 152, 210, 452
Hsp92I GRCGYC 1 cut(s) 452
Hsp92II CATG 2 cut(s) 129, 421
Kzo9I GATC 6 cut(s) 24, 72, 246, 325, 351, 412
LmnI GCTCC 3 cut(s) 268, 347, 366
LpnPI CCDG 7 cut(s) 152, 198, 230, 281, 342, 356, 414
Lsp1109I GCAGC 2 cut(s) 134, 326
LweI GCATC 1 cut(s) 271
MaeII ACGT 3 cut(s) 152, 210, 452
MalI GATC 6 cut(s) 26, 74, 248, 327, 353, 414
MboI GATC 6 cut(s) 24, 72, 246, 325, 351, 412
MboII GAAGA 2 cut(s) 34, 290
MflI RGATCY 2 cut(s) 246, 325
MhlI GDGCHC 1 cut(s) 363
MluCI AATT 1 cut(s) 131
MmeI TCCRAC 2 cut(s) 325, 449
MnlI CCTC 8 cut(s) 146, 181, 260, 298, 372, 391, 414, 465
MseI TTAA 2 cut(s) 80, 321
MspA1I CMGCKG 1 cut(s) 274
MspI CCGG 3 cut(s) 139, 185, 217
MwoI GCNNNNNNNGC 2 cut(s) 240, 305
NdeII GATC 6 cut(s) 24, 72, 246, 325, 351, 412
NlaIII CATG 2 cut(s) 129, 421
NlaIV GGNNCC 2 cut(s) 192, 264
NspI RCATGY 1 cut(s) 129
PaeI GCATGC 1 cut(s) 129
PaeR7I CTCGAG 1 cut(s) 395
PagI TCATGA 1 cut(s) 417
PflMI CCANNNNNTGG 2 cut(s) 259, 335
PkrI GCNGC 2 cut(s) 124, 341
PpuMI RGGWCCY 1 cut(s) 56
Psp5II RGGWCCY 1 cut(s) 56
PspN4I GGNNCC 2 cut(s) 192, 264
PspPI GGNCC 1 cut(s) 56
PspPPI RGGWCCY 1 cut(s) 56
PspXI VCTCGAGB 1 cut(s) 395
PsuI RGATCY 2 cut(s) 246, 325
RsaI GTAC 1 cut(s) 425
RsaNI GTAC 1 cut(s) 424
SaqAI TTAA 2 cut(s) 80, 321
SatI GCNGC 2 cut(s) 123, 340
Sau3AI GATC 6 cut(s) 24, 72, 246, 325, 351, 412
Sau96I GGNCC 1 cut(s) 56
SduI GDGCHC 1 cut(s) 363
SetI ASST 9 cut(s) 34, 61, 155, 196, 213, 375, 383, 425, 455
SfaNI GCATC 1 cut(s) 271
Sfr274I CTCGAG 1 cut(s) 395
SinI GGWCC 1 cut(s) 56
SlaI CTCGAG 1 cut(s) 395
SmlI CTYRAG 3 cut(s) 36, 376, 395
SmoI CTYRAG 3 cut(s) 36, 376, 395
SphI GCATGC 1 cut(s) 129
Sse9I AATT 1 cut(s) 131
SsiI CCGC 4 cut(s) 182, 274, 284, 308
TaaI ACNGT 1 cut(s) 117
TaiI ACGT 3 cut(s) 155, 213, 455
TaqI TCGA 2 cut(s) 98, 396
TasI AATT 1 cut(s) 131
Tru1I TTAA 2 cut(s) 80, 321
Tru9I TTAA 2 cut(s) 80, 321
TscAI CASTG 1 cut(s) 336
TseI GCWGC 2 cut(s) 122, 339
TspRI CASTG 1 cut(s) 336
Van91I CCANNNNNTGG 2 cut(s) 259, 335
VpaK11BI GGWCC 1 cut(s) 56
XapI RAATTY 1 cut(s) 131
XceI RCATGY 1 cut(s) 129
XhoI CTCGAG 1 cut(s) 395
ZraI GACGTC 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.