AT3G28710

Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014150
Physical Location & Seq
Reverse (-)
10772871 .. 10775809
2939 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G28710.1

Sequence Viewer

Length: 1056 bp
ATGTACGGATTCGAGGCGCTTACCTTCAACATCCATGGCGGATATTTGGAGGCGATCGTTAGAGGCCACCGTGCTGGGCTTCTCACCACCGCCGATTACAATAATCTATGTCAGTGTGAGAACCTAGACGATATCAAGATGCATCTCTCTGCCACCAAATACGGCTCTTATCTCCAAAACGAACCGTCACCTCTGCATACCACCACCATTGTGGAGAAGTGTACTCTTAAGCTTGTTGATGACTACAAGCATATGCTTTGCCAAGCTACTGAGCCAATGTCTACCTTTTTAGAGTACATCAGATATGGCCACATGATTGACAATGTCGTGCTCATTGTTACTGGAACCCTGCATGAGAGAGATGTTCAAGAGTTGATTGAGAAATGTCACCCTTTAGGCATGTTTGACAGTATTGCTACACTAGCAGTTGCTCAGAACATGCGGGAACTCTACCGGTTGGTGCTTGTGGATACTCCTTTGGCTCCATACTTTTCTGAATGCTTAACGTCAGAGGATCTCGATGACATGAACATAGAGATTATGAGGAACACCCTCTACAAAGCATACCTTGAGGATTTTTACAAGTTCTGTCAGAAACTTGGTGGCGCAACAGCGGAGATTATGTCTGACCTTTTGGCCTTTGAGGCCGATAGAAGAGCAGTGAATATCACTATCAACAGCATTGGCACTGAGCTCACAAGAGAAGACAGAAAGAAACTGTACTCTAACTTTGGTCTACTCTATCCATATGGTCACGAAGAGCTTGCTATCTGCGAGGATATAGACCAGGTTCGTGGTGTTATGGAGAAATATCCTCCGTATCAAGCTATATTCTCGAAGATGTCTTATGGAGAGAGCCAGATGCTCGACAAAGCATTTTATGAAGAAGAAGTCAGAAGGCTTTGCTTAGCCTTTGAGCAGCAGTTCCATTACGCGGTATTCTTTGCGTATATGAGGTTAAGAGAGCAGGAGATCAGGAACCTTATGTGGATATCTGAATGTGTTGCACAGAACCAGAAGTCCAGGATCCACGACAGTGTCGTCTACATGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0000325 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006885 GO:0007034 GO:0007035 GO:0008150 GO:0008324 GO:0008553 GO:0009506 GO:0009987 GO:0012505 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0015672 GO:0016020 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019725 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030054 GO:0030641 GO:0031090 GO:0032991 GO:0033176 GO:0034220 GO:0036442 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045851 GO:0046961 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0051452 GO:0051453 GO:0055044 GO:0055067 GO:0055080 GO:0055082 GO:0055085 GO:0065007 GO:0065008 GO:0071944 GO:0090662 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098796 GO:0098805 GO:0098852 GO:0099131 GO:0099132 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05203 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05203 map05323
Pfam Domains
Protein Families
vATP-synt_AC39

Protein Analysis

351

Amino Acids

40.79

Weight (kDa)

5.04

Isoelectric Point (pI)

50.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
vATP-synt_AC39 PF01992 16 - 344 1.4e-106 ATP synthase (C/AC39) subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014150)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28710 AT3G28715 AT3G28715
fragaria_vesca FvH4_6g49160
malus_domestica MD09G1048000.v1.1 MD17G1048200.v1.1
prunus_persica Prupe.3G271000_v2.0.a1 Prupe.3G271000_v2.0.a1
pyrus_communis pycom17g04290
rosa_chinensis RchiOBHm_Chr2g0169031
rosa_laevigata RLG00000021825
rosa_multiflora Rmu_ssc0000393.1_g000024
rosa_roxburghii Rroxscaffold_2G00082520
rosa_rugosa Rorug02G0536500
rosa_samantha Rh2AG607900 Rh2CG589500
rosa_wichuraiana Rw2G050410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1040
AccI GTMKAC 3 cut(s) 281, 736, 1044
AccII CGCG 1 cut(s) 935
AciI CCGC 5 cut(s) 39, 90, 442, 614, 935
AclWI GGATC 3 cut(s) 522, 1021, 1034
AcoI YGGCCR 1 cut(s) 307
AfaI GTAC 4 cut(s) 5, 223, 296, 722
AfiI CCNNNNNNNGG 1 cut(s) 934
AflII CTTAAG 1 cut(s) 227
AflIII ACRYGT 1 cut(s) 1047
AgeI ACCGGT 1 cut(s) 453
AgsI TTSAA 2 cut(s) 28, 368
AjnI CCWGG 2 cut(s) 786, 1022
AleI CACNNNNGTG 2 cut(s) 209, 1035
AloI GAACNNNNNNTCC 2 cut(s) 1004, 1036
AluBI AGCT 5 cut(s) 232, 266, 694, 763, 827
AluI AGCT 5 cut(s) 232, 266, 694, 763, 827
Alw21I GWGCWC 2 cut(s) 333, 696
AlwI GGATC 3 cut(s) 522, 1021, 1034
AoxI GGCC 4 cut(s) 64, 307, 636, 645
ApeKI GCWGC 1 cut(s) 919
AsiGI ACCGGT 1 cut(s) 453
AspLEI GCGC 2 cut(s) 19, 608
AsuHPI GGTGA 3 cut(s) 76, 180, 380
BalI TGGCCA 1 cut(s) 309
BamHI GGATCC 1 cut(s) 1026
BanII GRGCYC 1 cut(s) 696
BbsI GAAGAC 1 cut(s) 711
Bbv12I GWGCWC 2 cut(s) 333, 696
BbvI GCAGC 1 cut(s) 931
BceAI ACGGC 1 cut(s) 178
BcgI CGANNNNNNTGC 2 cut(s) 746, 780
BciT130I CCWGG 2 cut(s) 788, 1024
BciVI GTATCC 1 cut(s) 463
BfaI CTAG 2 cut(s) 125, 422
BfoI RGCGCY 1 cut(s) 20
BfrI CTTAAG 1 cut(s) 227
BfuI GTATCC 1 cut(s) 463
BglI GCCNNNNNGGC 1 cut(s) 644
BisI GCNGC 1 cut(s) 920
BlpI GCTNAGC 1 cut(s) 907
BlsI GCNGC 1 cut(s) 921
Bme1390I CCNGG 2 cut(s) 788, 1024
BmiI GGNNCC 4 cut(s) 346, 483, 980, 1028
BmrFI CCNGG 2 cut(s) 788, 1024
BmsI GCATC 3 cut(s) 129, 151, 852
BpiI GAAGAC 1 cut(s) 711
Bpu1102I GCTNAGC 1 cut(s) 907
BpuEI CTTGAG 1 cut(s) 590
BsaJI CCNNGG 1 cut(s) 34
BsaWI WCCGGW 1 cut(s) 453
BsaXI ACNNNNNCTCC 2 cut(s) 608, 638
Bsc4I CCNNNNNNNGG 1 cut(s) 934
Bse118I RCCGGY 1 cut(s) 453
Bse1I ACTGG 1 cut(s) 346
BseBI CCWGG 2 cut(s) 788, 1024
BseDI CCNNGG 1 cut(s) 34
BseGI GGATG 1 cut(s) 30
BseLI CCNNNNNNNGG 1 cut(s) 934
BseMII CTCAG 3 cut(s) 261, 446, 681
BseNI ACTGG 1 cut(s) 346
BseXI GCAGC 1 cut(s) 931
BseYI CCCAGC 1 cut(s) 74
Bsh1236I CGCG 1 cut(s) 935
Bsh1285I CGRYCG 1 cut(s) 57
BshFI GGCC 4 cut(s) 66, 309, 638, 647
BshTI ACCGGT 1 cut(s) 453
BsiEI CGRYCG 1 cut(s) 57
BsiHKAI GWGCWC 2 cut(s) 333, 696
BsiSI CCGG 1 cut(s) 454
BslI CCNNNNNNNGG 1 cut(s) 934
BsmI GAATGC 1 cut(s) 503
BsnI GGCC 4 cut(s) 66, 309, 638, 647
Bsp1286I GDGCHC 2 cut(s) 333, 696
Bsp143I GATC 4 cut(s) 54, 514, 972, 1026
Bsp1720I GCTNAGC 1 cut(s) 907
Bsp19I CCATGG 1 cut(s) 34
BspACI CCGC 5 cut(s) 39, 90, 442, 614, 935
BspANI GGCC 4 cut(s) 66, 309, 638, 647
BspCNI CTCAG 3 cut(s) 262, 445, 682
BspFNI CGCG 1 cut(s) 935
BspLI GGNNCC 4 cut(s) 346, 483, 980, 1028
BspPI GGATC 3 cut(s) 522, 1021, 1034
BspQI GCTCTTC 2 cut(s) 649, 753
BspTI CTTAAG 1 cut(s) 227
BsrFI RCCGGY 1 cut(s) 453
BsrI ACTGG 1 cut(s) 346
BssAI RCCGGY 1 cut(s) 453
BssECI CCNNGG 1 cut(s) 34
BssMI GATC 4 cut(s) 54, 514, 972, 1026
BssT1I CCWWGG 1 cut(s) 34
Bst2UI CCWGG 2 cut(s) 788, 1024
Bst4CI ACNGT 5 cut(s) 71, 186, 410, 720, 1037
Bst6I CTCTTC 2 cut(s) 649, 753
BstAFI CTTAAG 1 cut(s) 227
BstC8I GCNNGC 1 cut(s) 765
BstDEI CTNAG 4 cut(s) 270, 432, 690, 907
BstDSI CCRYGG 1 cut(s) 34
BstF5I GGATG 1 cut(s) 30
BstFNI CGCG 1 cut(s) 935
BstH2I RGCGCY 1 cut(s) 20
BstHHI GCGC 2 cut(s) 19, 608
BstKTI GATC 4 cut(s) 57, 517, 975, 1029
BstMBI GATC 4 cut(s) 54, 514, 972, 1026
BstMCI CGRYCG 1 cut(s) 57
BstMWI GCNNNNNNNGC 2 cut(s) 422, 644
BstNI CCWGG 2 cut(s) 788, 1024
BstNSI RCATGY 3 cut(s) 403, 442, 1051
BstSCI CCNGG 2 cut(s) 786, 1022
BstUI CGCG 1 cut(s) 935
BstV1I GCAGC 1 cut(s) 931
BstV2I GAAGAC 1 cut(s) 711
BstX2I RGATCY 2 cut(s) 514, 1026
BstXI CCANNNNNNTGG 3 cut(s) 74, 211, 794
BstYI RGATCY 2 cut(s) 514, 1026
BsuI GTATCC 1 cut(s) 463
BsuRI GGCC 4 cut(s) 66, 309, 638, 647
BtgI CCRYGG 1 cut(s) 34
BtsCI GGATG 1 cut(s) 30
BtsI GCAGTG 1 cut(s) 666
BtsIMutI CAGTG 4 cut(s) 119, 666, 687, 1042
Cac8I GCNNGC 1 cut(s) 765
CfoI GCGC 2 cut(s) 19, 608
Cfr10I RCCGGY 1 cut(s) 453
CsiI ACCWGGT 1 cut(s) 786
Csp6I GTAC 4 cut(s) 4, 222, 295, 721
CspAI ACCGGT 1 cut(s) 453
CspCI CAANNNNNGTGG 2 cut(s) 190, 225
CviAII CATG 7 cut(s) 35, 313, 353, 400, 439, 526, 1048
CviQI GTAC 4 cut(s) 4, 222, 295, 721
DdeI CTNAG 4 cut(s) 270, 432, 690, 907
DpnI GATC 4 cut(s) 56, 516, 974, 1028
DpnII GATC 4 cut(s) 54, 514, 972, 1026
DrdI GACNNNNNNGTC 1 cut(s) 1040
DseDI GACNNNNNNGTC 1 cut(s) 1040
EaeI YGGCCR 1 cut(s) 307
Eam1104I CTCTTC 2 cut(s) 649, 753
EarI CTCTTC 2 cut(s) 649, 753
EciI GGCGGA 1 cut(s) 54
Ecl136II GAGCTC 1 cut(s) 694
Eco130I CCWWGG 1 cut(s) 34
Eco24I GRGCYC 1 cut(s) 696
Eco32I GATATC 2 cut(s) 133, 993
Eco53kI GAGCTC 1 cut(s) 694
EcoICRI GAGCTC 1 cut(s) 694
EcoRII CCWGG 2 cut(s) 786, 1022
EcoRV GATATC 2 cut(s) 133, 993
EcoT14I CCWWGG 1 cut(s) 34
EcoT22I ATGCAT 1 cut(s) 144
EcoT38I GRGCYC 1 cut(s) 696
ErhI CCWWGG 1 cut(s) 34
FaeI CATG 7 cut(s) 38, 316, 356, 403, 442, 529, 1051
FalI AAGNNNNNCTT 2 cut(s) 552, 584
FatI CATG 7 cut(s) 34, 312, 352, 399, 438, 525, 1047
FauI CCCGC 1 cut(s) 435
FauNDI CATATG 2 cut(s) 252, 748
FblI GTMKAC 3 cut(s) 281, 736, 1044
Fnu4HI GCNGC 1 cut(s) 920
FokI GGATG 1 cut(s) 17
FriOI GRGCYC 1 cut(s) 696
Fsp4HI GCNGC 1 cut(s) 920
FspBI CTAG 2 cut(s) 125, 422
GlaI GCGC 2 cut(s) 18, 607
GluI GCNGC 1 cut(s) 920
GsaI CCCAGC 1 cut(s) 78
HaeII RGCGCY 1 cut(s) 20
HaeIII GGCC 4 cut(s) 66, 309, 638, 647
HapII CCGG 1 cut(s) 454
HhaI GCGC 2 cut(s) 19, 608
Hin1II CATG 7 cut(s) 38, 316, 356, 403, 442, 529, 1051
Hin6I GCGC 2 cut(s) 17, 606
HinP1I GCGC 2 cut(s) 17, 606
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 1 cut(s) 9
HpaII CCGG 1 cut(s) 454
HphI GGTGA 3 cut(s) 76, 180, 380
Hpy166II GTNNAC 4 cut(s) 222, 282, 737, 1045
Hpy188I TCNGA 9 cut(s) 302, 435, 496, 511, 594, 628, 896, 997, 1055
Hpy188III TCNNGA 6 cut(s) 136, 368, 518, 755, 835, 976
Hpy8I GTNNAC 4 cut(s) 222, 282, 737, 1045
HpyAV CCTTC 2 cut(s) 34, 891
HpyCH4III ACNGT 5 cut(s) 71, 186, 410, 720, 1037
HpyCH4IV ACGT 1 cut(s) 506
HpyCH4V TGCA 4 cut(s) 142, 196, 352, 1007
HpyF10VI GCNNNNNNNGC 2 cut(s) 422, 644
HpyF3I CTNAG 4 cut(s) 270, 432, 690, 907
HpySE526I ACGT 1 cut(s) 506
Hsp92II CATG 7 cut(s) 38, 316, 356, 403, 442, 529, 1051
HspAI GCGC 2 cut(s) 17, 606
Kzo9I GATC 4 cut(s) 54, 514, 972, 1026
LguI GCTCTTC 2 cut(s) 649, 753
LmnI GCTCC 1 cut(s) 487
Lsp1109I GCAGC 1 cut(s) 931
LweI GCATC 3 cut(s) 129, 151, 852
MabI ACCWGGT 1 cut(s) 786
MaeI CTAG 2 cut(s) 125, 422
MaeII ACGT 1 cut(s) 506
MaeIII GTNAC 4 cut(s) 186, 337, 386, 752
MalI GATC 4 cut(s) 56, 516, 974, 1028
MboI GATC 4 cut(s) 54, 514, 972, 1026
MboII GAAGA 6 cut(s) 666, 716, 770, 850, 896, 899
MflI RGATCY 2 cut(s) 514, 1026
MhlI GDGCHC 2 cut(s) 333, 696
MlsI TGGCCA 1 cut(s) 309
MluNI TGGCCA 1 cut(s) 309
Mox20I TGGCCA 1 cut(s) 309
Mph1103I ATGCAT 1 cut(s) 144
MscI TGGCCA 1 cut(s) 309
MseI TTAA 3 cut(s) 228, 503, 959
MslI CAYNNNNRTG 2 cut(s) 209, 1035
Msp20I TGGCCA 1 cut(s) 309
MspA1I CMGCKG 1 cut(s) 614
MspCI CTTAAG 1 cut(s) 227
MspI CCGG 1 cut(s) 454
MspR9I CCNGG 2 cut(s) 788, 1024
Mva1269I GAATGC 1 cut(s) 503
MvaI CCWGG 2 cut(s) 788, 1024
MvnI CGCG 1 cut(s) 935
MwoI GCNNNNNNNGC 2 cut(s) 422, 644
NcoI CCATGG 1 cut(s) 34
NdeI CATATG 2 cut(s) 252, 748
NdeII GATC 4 cut(s) 54, 514, 972, 1026
NlaIII CATG 7 cut(s) 38, 316, 356, 403, 442, 529, 1051
NlaIV GGNNCC 4 cut(s) 346, 483, 980, 1028
NmuCI GTSAC 3 cut(s) 186, 386, 752
NsiI ATGCAT 1 cut(s) 144
NspI RCATGY 3 cut(s) 403, 442, 1051
OliI CACNNNNGTG 2 cut(s) 209, 1035
PciI ACATGT 1 cut(s) 1047
PciSI GCTCTTC 2 cut(s) 649, 753
PcsI WCGNNNNNNNCGW 1 cut(s) 1038
PctI GAATGC 1 cut(s) 503
PfeI GAWTC 1 cut(s) 9
PflFI GACNNNGTC 2 cut(s) 323, 1037
PfoI TCCNGGA 1 cut(s) 1022
PinAI ACCGGT 1 cut(s) 453
PkrI GCNGC 1 cut(s) 921
Ple19I CGATCG 1 cut(s) 57
PscI ACATGT 1 cut(s) 1047
Psp124BI GAGCTC 1 cut(s) 696
Psp6I CCWGG 2 cut(s) 786, 1022
PspFI CCCAGC 1 cut(s) 74
PspGI CCWGG 2 cut(s) 786, 1022
PspN4I GGNNCC 4 cut(s) 346, 483, 980, 1028
PsrI GAACNNNNNNTAC 2 cut(s) 539, 571
PsuI RGATCY 2 cut(s) 514, 1026
PsyI GACNNNGTC 2 cut(s) 323, 1037
PvuI CGATCG 1 cut(s) 57
RsaI GTAC 4 cut(s) 5, 223, 296, 722
RsaNI GTAC 4 cut(s) 4, 222, 295, 721
RseI CAYNNNNRTG 2 cut(s) 209, 1035
SacI GAGCTC 1 cut(s) 696
SapI GCTCTTC 2 cut(s) 649, 753
SaqAI TTAA 3 cut(s) 228, 503, 959
SatI GCNGC 1 cut(s) 920
Sau3AI GATC 4 cut(s) 54, 514, 972, 1026
ScrFI CCNGG 2 cut(s) 788, 1024
SduI GDGCHC 2 cut(s) 333, 696
SexAI ACCWGGT 1 cut(s) 786
SfaNI GCATC 3 cut(s) 129, 151, 852
SfiI GGCCNNNNNGGCC 1 cut(s) 644
SmiMI CAYNNNNRTG 2 cut(s) 209, 1035
SmlI CTYRAG 2 cut(s) 227, 569
SmoI CTYRAG 2 cut(s) 227, 569
SsiI CCGC 5 cut(s) 39, 90, 442, 614, 935
SspMI CTAG 2 cut(s) 125, 422
SstI GAGCTC 1 cut(s) 696
StyD4I CCNGG 2 cut(s) 786, 1022
StyI CCWWGG 1 cut(s) 34
TaaI ACNGT 5 cut(s) 71, 186, 410, 720, 1037
TaiI ACGT 1 cut(s) 509
TaqI TCGA 4 cut(s) 12, 519, 836, 867
TatI WGTACW 3 cut(s) 221, 294, 720
TfiI GAWTC 1 cut(s) 9
Tru1I TTAA 3 cut(s) 228, 503, 959
Tru9I TTAA 3 cut(s) 228, 503, 959
TscAI CASTG 4 cut(s) 119, 666, 694, 1042
TseFI GTSAC 3 cut(s) 186, 386, 752
TseI GCWGC 1 cut(s) 919
Tsp45I GTSAC 3 cut(s) 186, 386, 752
TspDTI ATGAA 2 cut(s) 542, 897
TspGWI ACGGA 2 cut(s) 21, 807
TspRI CASTG 4 cut(s) 119, 666, 694, 1042
Tth111I GACNNNGTC 2 cut(s) 323, 1037
Vha464I CTTAAG 1 cut(s) 227
XceI RCATGY 3 cut(s) 403, 442, 1051
XcmI CCANNNNNNNNNTGG 1 cut(s) 208
XmiI GTMKAC 3 cut(s) 281, 736, 1044
XspI CTAG 2 cut(s) 125, 422
Zsp2I ATGCAT 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.