AT3G28715

Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014150
Physical Location & Seq
Forward (+)
10777837 .. 10780664
2828 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G28715.2

Sequence Viewer

Length: 1056 bp
ATGTACGGATTCGAGGCGCTTACGTTCAATATCCATGGGGGATACTTGGAGGCGATCGTCAGAGGCCACCGTGCTGGGCTTCTCACCACCGCCGATTACAACAATCTATGTCAGTGTGAGAACCTAGACGATATCAAGATGCATCTCTCTGCCACCAAATACGGTCCTTACCTCCAAAACGAACCATCACCTCTGCATACCACTACAATTGTGGAGAAGTGTACGCTCAAGCTTGTTGATGACTACAAGCATATGCTTTGCCAAGCTACTGAGCCAATGTCTACCTTTTTAGAGTACATCAGATATGGTCATATGATTGACAATGTCGTGCTCATTGTTACTGGAACCCTTCACGAGAGAGATGTTCAAGAGTTGATCGAGAAATGTCACCCTTTAGGCATGTTTGACAGTATTGCTACACTAGCTGTTGCTCAGAACATGCGGGAACTCTATAGGTTGGTGCTTGTGGACACTCCTCTGGCTCCATACTTTTCTGAATGCCTAACATCAGAGGATCTCGATGACATGAACATAGAGATTATGAGGAATACCCTCTACAAAGCATACCTTGAGGATTTTTACAACTTCTGTCAGAAACTTGGTGGCGCAACAGCAGAGATTATGTCTGACCTTTTGGCCTTTGAAGCCGACAGGAGAGCAGTGAATATCACTATCAACAGCATTGGCACTGAGCTCACAAGAGAAGACAGGAAGAAACTGTACTCCAACTTTGGTCTCCTCTATCCATATGGCCACGAGGAGCTTGCTATCTGTGAAGACATAGATCAGGTTCGTGGTGTTATGGAAAAATACCCTCCGTATCAAGCTATATTCTCCAAGATGTCTTATGGAGAAAGCCAGATGCTCGACAAGGCATTTTATGAAGAAGAAGTCAGAAGGCTTTGCTTAGCCTTTGAGCAGCAGTTCCATTACGCGGTATTCTTTGCGTATATGAGGTTGCGGGAGCAGGAGATCAGGAACCTGATGTGGATATCTGAATGTGTTGCACAGAACCAGAAGTCCAGGATCCACGATAGTGTGGTCTACATGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0000325 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006885 GO:0007034 GO:0007035 GO:0008150 GO:0008324 GO:0008553 GO:0009506 GO:0009987 GO:0012505 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0015672 GO:0016020 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019725 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030054 GO:0030641 GO:0031090 GO:0032991 GO:0033176 GO:0034220 GO:0036442 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045851 GO:0046961 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0051452 GO:0051453 GO:0055044 GO:0055067 GO:0055080 GO:0055082 GO:0055085 GO:0065007 GO:0065008 GO:0071944 GO:0090662 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098796 GO:0098805 GO:0098852 GO:0099131 GO:0099132 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05203 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05203 map05323
Pfam Domains
Protein Families
vATP-synt_AC39

Protein Analysis

351

Amino Acids

40.79

Weight (kDa)

4.99

Isoelectric Point (pI)

50.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
vATP-synt_AC39 PF01992 16 - 336 2.2e-99 ATP synthase (C/AC39) subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014150)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28710 AT3G28715 AT3G28715
fragaria_vesca FvH4_6g49160
malus_domestica MD09G1048000.v1.1 MD17G1048200.v1.1
prunus_persica Prupe.3G271000_v2.0.a1 Prupe.3G271000_v2.0.a1
pyrus_communis pycom17g04290
rosa_chinensis RchiOBHm_Chr2g0169031
rosa_laevigata RLG00000021825
rosa_multiflora Rmu_ssc0000393.1_g000024
rosa_roxburghii Rroxscaffold_2G00082520
rosa_rugosa Rorug02G0536500
rosa_samantha Rh2AG607900 Rh2CG589500
rosa_wichuraiana Rw2G050410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 281, 1044
AccII CGCG 1 cut(s) 935
AciI CCGC 4 cut(s) 90, 442, 935, 961
AclWI GGATC 3 cut(s) 522, 1021, 1034
AcoI YGGCCR 1 cut(s) 751
AfaI GTAC 4 cut(s) 5, 223, 296, 722
AfiI CCNNNNNNNGG 1 cut(s) 934
AflIII ACRYGT 1 cut(s) 1047
AgsI TTSAA 3 cut(s) 28, 368, 644
AjnI CCWGG 1 cut(s) 1022
AleI CACNNNNGTG 1 cut(s) 1035
AloI GAACNNNNNNTCC 2 cut(s) 1004, 1036
AluBI AGCT 6 cut(s) 232, 266, 425, 694, 763, 827
AluI AGCT 6 cut(s) 232, 266, 425, 694, 763, 827
Alw21I GWGCWC 2 cut(s) 333, 696
Alw26I GTCTC 1 cut(s) 740
AlwI GGATC 3 cut(s) 522, 1021, 1034
AoxI GGCC 3 cut(s) 64, 636, 751
ApeKI GCWGC 1 cut(s) 919
AspLEI GCGC 2 cut(s) 19, 608
AspS9I GGNCC 1 cut(s) 164
AsuHPI GGTGA 3 cut(s) 76, 180, 380
AvaII GGWCC 1 cut(s) 164
BalI TGGCCA 1 cut(s) 753
BamHI GGATCC 1 cut(s) 1026
BanII GRGCYC 1 cut(s) 696
BarI GAAGNNNNNNTAC 2 cut(s) 704, 736
BauI CACGAG 2 cut(s) 353, 755
BbsI GAAGAC 2 cut(s) 711, 783
Bbv12I GWGCWC 2 cut(s) 333, 696
BbvI GCAGC 1 cut(s) 931
BccI CCATC 1 cut(s) 193
BcgI CGANNNNNNTGC 2 cut(s) 746, 780
BciT130I CCWGG 1 cut(s) 1024
BciVI GTATCC 1 cut(s) 35
BcoDI GTCTC 1 cut(s) 740
BfaI CTAG 2 cut(s) 125, 422
BfmI CTRYAG 1 cut(s) 451
BfoI RGCGCY 1 cut(s) 20
BfuI GTATCC 1 cut(s) 35
BisI GCNGC 1 cut(s) 920
BlpI GCTNAGC 1 cut(s) 907
BlsI GCNGC 1 cut(s) 921
Bme1390I CCNGG 1 cut(s) 1024
Bme18I GGWCC 1 cut(s) 164
BmgT120I GGNCC 1 cut(s) 164
BmiI GGNNCC 4 cut(s) 346, 483, 980, 1028
BmrFI CCNGG 1 cut(s) 1024
BmsI GCATC 3 cut(s) 129, 151, 852
BpiI GAAGAC 2 cut(s) 711, 783
Bpu1102I GCTNAGC 1 cut(s) 907
BpuEI CTTGAG 2 cut(s) 212, 590
BsaI GGTCTC 1 cut(s) 740
BsaJI CCNNGG 1 cut(s) 34
Bsc4I CCNNNNNNNGG 1 cut(s) 934
Bse1I ACTGG 1 cut(s) 346
BseBI CCWGG 1 cut(s) 1024
BseDI CCNNGG 1 cut(s) 34
BseLI CCNNNNNNNGG 1 cut(s) 934
BseMII CTCAG 3 cut(s) 261, 446, 681
BseNI ACTGG 1 cut(s) 346
BseRI GAGGAG 3 cut(s) 465, 728, 773
BseXI GCAGC 1 cut(s) 931
BseYI CCCAGC 1 cut(s) 74
Bsh1236I CGCG 1 cut(s) 935
Bsh1285I CGRYCG 1 cut(s) 57
BshFI GGCC 3 cut(s) 66, 638, 753
BsiEI CGRYCG 1 cut(s) 57
BsiHKAI GWGCWC 2 cut(s) 333, 696
BslI CCNNNNNNNGG 1 cut(s) 934
BsmAI GTCTC 1 cut(s) 740
BsmI GAATGC 1 cut(s) 503
BsnI GGCC 3 cut(s) 66, 638, 753
Bso31I GGTCTC 1 cut(s) 740
Bsp1286I GDGCHC 2 cut(s) 333, 696
Bsp143I GATC 6 cut(s) 54, 375, 514, 784, 972, 1026
Bsp1720I GCTNAGC 1 cut(s) 907
Bsp19I CCATGG 1 cut(s) 34
BspACI CCGC 4 cut(s) 90, 442, 935, 961
BspANI GGCC 3 cut(s) 66, 638, 753
BspCNI CTCAG 3 cut(s) 262, 445, 682
BspFNI CGCG 1 cut(s) 935
BspLI GGNNCC 4 cut(s) 346, 483, 980, 1028
BspPI GGATC 3 cut(s) 522, 1021, 1034
BspTNI GGTCTC 1 cut(s) 740
BsrI ACTGG 1 cut(s) 346
BssECI CCNNGG 1 cut(s) 34
BssMI GATC 6 cut(s) 54, 375, 514, 784, 972, 1026
BssSI CACGAG 2 cut(s) 353, 755
BssT1I CCWWGG 1 cut(s) 34
Bst2BI CACGAG 2 cut(s) 353, 755
Bst2UI CCWGG 1 cut(s) 1024
Bst4CI ACNGT 4 cut(s) 71, 164, 410, 720
BstC8I GCNNGC 1 cut(s) 765
BstDEI CTNAG 4 cut(s) 270, 432, 690, 907
BstDSI CCRYGG 1 cut(s) 34
BstFNI CGCG 1 cut(s) 935
BstH2I RGCGCY 1 cut(s) 20
BstHHI GCGC 2 cut(s) 19, 608
BstKTI GATC 6 cut(s) 57, 378, 517, 787, 975, 1029
BstMAI GTCTC 1 cut(s) 740
BstMBI GATC 6 cut(s) 54, 375, 514, 784, 972, 1026
BstMCI CGRYCG 1 cut(s) 57
BstMWI GCNNNNNNNGC 2 cut(s) 422, 644
BstNI CCWGG 1 cut(s) 1024
BstNSI RCATGY 3 cut(s) 403, 442, 1051
BstSCI CCNGG 1 cut(s) 1022
BstSFI CTRYAG 1 cut(s) 451
BstUI CGCG 1 cut(s) 935
BstV1I GCAGC 1 cut(s) 931
BstV2I GAAGAC 2 cut(s) 711, 783
BstX2I RGATCY 2 cut(s) 514, 1026
BstXI CCANNNNNNTGG 1 cut(s) 74
BstYI RGATCY 2 cut(s) 514, 1026
BsuI GTATCC 1 cut(s) 35
BsuRI GGCC 3 cut(s) 66, 638, 753
BtgI CCRYGG 1 cut(s) 34
BtsI GCAGTG 1 cut(s) 666
BtsIMutI CAGTG 3 cut(s) 119, 666, 687
Cac8I GCNNGC 1 cut(s) 765
CfoI GCGC 2 cut(s) 19, 608
Cfr13I GGNCC 1 cut(s) 164
Csp6I GTAC 4 cut(s) 4, 222, 295, 721
CspCI CAANNNNNGTGG 2 cut(s) 190, 225
CviAII CATG 5 cut(s) 35, 400, 439, 526, 1048
CviQI GTAC 4 cut(s) 4, 222, 295, 721
DdeI CTNAG 4 cut(s) 270, 432, 690, 907
DpnI GATC 6 cut(s) 56, 377, 516, 786, 974, 1028
DpnII GATC 6 cut(s) 54, 375, 514, 784, 972, 1026
EaeI YGGCCR 1 cut(s) 751
Ecl136II GAGCTC 1 cut(s) 694
Eco130I CCWWGG 1 cut(s) 34
Eco24I GRGCYC 1 cut(s) 696
Eco31I GGTCTC 1 cut(s) 740
Eco32I GATATC 2 cut(s) 133, 993
Eco47I GGWCC 1 cut(s) 164
Eco53kI GAGCTC 1 cut(s) 694
EcoICRI GAGCTC 1 cut(s) 694
EcoRII CCWGG 1 cut(s) 1022
EcoRV GATATC 2 cut(s) 133, 993
EcoT14I CCWWGG 1 cut(s) 34
EcoT22I ATGCAT 1 cut(s) 144
EcoT38I GRGCYC 1 cut(s) 696
ErhI CCWWGG 1 cut(s) 34
FaeI CATG 5 cut(s) 38, 403, 442, 529, 1051
FalI AAGNNNNNCTT 2 cut(s) 552, 584
FatI CATG 5 cut(s) 34, 399, 438, 525, 1047
FauI CCCGC 2 cut(s) 435, 954
FauNDI CATATG 3 cut(s) 252, 312, 748
FblI GTMKAC 2 cut(s) 281, 1044
Fnu4HI GCNGC 1 cut(s) 920
FriOI GRGCYC 1 cut(s) 696
Fsp4HI GCNGC 1 cut(s) 920
FspBI CTAG 2 cut(s) 125, 422
GlaI GCGC 2 cut(s) 18, 607
GluI GCNGC 1 cut(s) 920
GsaI CCCAGC 1 cut(s) 78
HaeII RGCGCY 1 cut(s) 20
HaeIII GGCC 3 cut(s) 66, 638, 753
HhaI GCGC 2 cut(s) 19, 608
Hin1II CATG 5 cut(s) 38, 403, 442, 529, 1051
Hin6I GCGC 2 cut(s) 17, 606
HinP1I GCGC 2 cut(s) 17, 606
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 1 cut(s) 9
HphI GGTGA 3 cut(s) 76, 180, 380
Hpy166II GTNNAC 4 cut(s) 222, 282, 469, 1045
Hpy188III TCNNGA 6 cut(s) 136, 353, 368, 379, 518, 976
Hpy8I GTNNAC 4 cut(s) 222, 282, 469, 1045
HpyAV CCTTC 2 cut(s) 359, 891
HpyCH4III ACNGT 4 cut(s) 71, 164, 410, 720
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 3 cut(s) 142, 196, 1007
HpyF10VI GCNNNNNNNGC 2 cut(s) 422, 644
HpyF3I CTNAG 4 cut(s) 270, 432, 690, 907
HpySE526I ACGT 1 cut(s) 23
Hsp92II CATG 5 cut(s) 38, 403, 442, 529, 1051
HspAI GCGC 2 cut(s) 17, 606
Kzo9I GATC 6 cut(s) 54, 375, 514, 784, 972, 1026
LmnI GCTCC 3 cut(s) 487, 760, 964
Lsp1109I GCAGC 1 cut(s) 931
LweI GCATC 3 cut(s) 129, 151, 852
MaeI CTAG 2 cut(s) 125, 422
MaeII ACGT 1 cut(s) 23
MaeIII GTNAC 2 cut(s) 337, 386
MalI GATC 6 cut(s) 56, 377, 516, 786, 974, 1028
MboI GATC 6 cut(s) 54, 375, 514, 784, 972, 1026
MboII GAAGA 5 cut(s) 716, 724, 788, 896, 899
MfeI CAATTG 1 cut(s) 207
MflI RGATCY 2 cut(s) 514, 1026
MhlI GDGCHC 2 cut(s) 333, 696
MlsI TGGCCA 1 cut(s) 753
MluCI AATT 1 cut(s) 207
MluNI TGGCCA 1 cut(s) 753
MmeI TCCRAC 1 cut(s) 750
Mox20I TGGCCA 1 cut(s) 753
Mph1103I ATGCAT 1 cut(s) 144
MscI TGGCCA 1 cut(s) 753
MslI CAYNNNNRTG 1 cut(s) 1035
Msp20I TGGCCA 1 cut(s) 753
MspR9I CCNGG 1 cut(s) 1024
MunI CAATTG 1 cut(s) 207
Mva1269I GAATGC 1 cut(s) 503
MvaI CCWGG 1 cut(s) 1024
MvnI CGCG 1 cut(s) 935
MwoI GCNNNNNNNGC 2 cut(s) 422, 644
NcoI CCATGG 1 cut(s) 34
NdeI CATATG 3 cut(s) 252, 312, 748
NdeII GATC 6 cut(s) 54, 375, 514, 784, 972, 1026
NlaIII CATG 5 cut(s) 38, 403, 442, 529, 1051
NlaIV GGNNCC 4 cut(s) 346, 483, 980, 1028
NmuCI GTSAC 1 cut(s) 386
NsiI ATGCAT 1 cut(s) 144
NspI RCATGY 3 cut(s) 403, 442, 1051
OliI CACNNNNGTG 1 cut(s) 1035
PciI ACATGT 1 cut(s) 1047
PctI GAATGC 1 cut(s) 503
PfeI GAWTC 1 cut(s) 9
PflFI GACNNNGTC 1 cut(s) 323
PfoI TCCNGGA 1 cut(s) 1022
PkrI GCNGC 1 cut(s) 921
Ple19I CGATCG 1 cut(s) 57
PscI ACATGT 1 cut(s) 1047
Psp124BI GAGCTC 1 cut(s) 696
Psp6I CCWGG 1 cut(s) 1022
PspFI CCCAGC 1 cut(s) 74
PspGI CCWGG 1 cut(s) 1022
PspN4I GGNNCC 4 cut(s) 346, 483, 980, 1028
PspPI GGNCC 1 cut(s) 164
PsuI RGATCY 2 cut(s) 514, 1026
PsyI GACNNNGTC 1 cut(s) 323
PvuI CGATCG 1 cut(s) 57
RsaI GTAC 4 cut(s) 5, 223, 296, 722
RsaNI GTAC 4 cut(s) 4, 222, 295, 721
RseI CAYNNNNRTG 1 cut(s) 1035
SacI GAGCTC 1 cut(s) 696
SatI GCNGC 1 cut(s) 920
Sau3AI GATC 6 cut(s) 54, 375, 514, 784, 972, 1026
Sau96I GGNCC 1 cut(s) 164
ScrFI CCNGG 1 cut(s) 1024
SduI GDGCHC 2 cut(s) 333, 696
SfaNI GCATC 3 cut(s) 129, 151, 852
SfcI CTRYAG 1 cut(s) 451
SinI GGWCC 1 cut(s) 164
SmiMI CAYNNNNRTG 1 cut(s) 1035
SmlI CTYRAG 2 cut(s) 227, 569
SmoI CTYRAG 2 cut(s) 227, 569
Sse9I AATT 1 cut(s) 207
SsiI CCGC 4 cut(s) 90, 442, 935, 961
SspMI CTAG 2 cut(s) 125, 422
SstI GAGCTC 1 cut(s) 696
StyD4I CCNGG 1 cut(s) 1022
StyI CCWWGG 1 cut(s) 34
TaaI ACNGT 4 cut(s) 71, 164, 410, 720
TaiI ACGT 1 cut(s) 26
TaqI TCGA 4 cut(s) 12, 378, 519, 867
TasI AATT 1 cut(s) 207
TatI WGTACW 2 cut(s) 294, 720
TfiI GAWTC 1 cut(s) 9
TscAI CASTG 3 cut(s) 119, 666, 694
TseFI GTSAC 1 cut(s) 386
TseI GCWGC 1 cut(s) 919
Tsp45I GTSAC 1 cut(s) 386
TspDTI ATGAA 2 cut(s) 542, 897
TspGWI ACGGA 2 cut(s) 21, 807
TspRI CASTG 3 cut(s) 119, 666, 694
Tth111I GACNNNGTC 1 cut(s) 323
VpaK11BI GGWCC 1 cut(s) 164
XceI RCATGY 3 cut(s) 403, 442, 1051
XcmI CCANNNNNNNNNTGG 1 cut(s) 208
XmiI GTMKAC 2 cut(s) 281, 1044
XspI CTAG 2 cut(s) 125, 422
Zsp2I ATGCAT 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.