AT3G44990

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014989
Physical Location & Seq
Reverse (-)
16446935 .. 16448844
1910 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G44990.1

Sequence Viewer

Length: 882 bp
ATGGCTTTGTCTCTTATCTTTCTAGCTCTCTTAGTGTTGTGCCCTAGTAGTGGTCACAGCCAACGTTCTCCTTCACCGGGATACTACCCGAGTTCCCGAGTACCGACTTCACCTTTTGATCGTGAATTCCGTACTTTATGGGGCTCTCAACACCAACGTAGAGAGCAAGACGTTGTCACTCTTTGGCTCGACAAATCCACTGGGAGTGGGTTCAAGTCTCTTCGTCCATACCGGTCGGGCTACTTTGGTGCTTCCATTAAGCTCCAACCAGGCTTCACTGCTGGAGTGGATACATCCCTCTATCTCTCAAACAACCAAGAGCATCCTGGAGACCACGATGAGGTCGATATCGAGTTTCTAGGGACAACGCCAGGGAAGCCTTATTCCCTTCAAACGAATGTCTTCGTTAGGGGAAGTGGCGACCGAAATGTCATTGGAAGAGAAATGAAATTTACCTTGTGGTTTGACCCTACTCAAGATTTTCACCATTACGCAATTTTGTGGAACCCTAACCAAATTGTATTCTTTGTAGACGATGTACCGATACGTACATATAATAGAAAGAATGAAGCTATATTCCCCACAAGACCGATGTGGGTTTACGGATCGATATGGGATGCATCGGACTGGGCCACAGAAAATGGAAGGATCAAAGCCGACTATCGATACCAACCATTTGTGGCTAAGTACAAAAACTTTAAGCTAGCGGGATGCACAGCGGATAGCTCTAGCTCATGCAGACCGCCATCGCCTGCACCCATGCGCAACCGCGGGTTGAGCCGGCAGCAGATGGCGGCATTGACATGGGCACAGAGGAACTTCTTGGTCTATAACTATTGCCATGATCCGAAAAGAGACCATACCCAAACACCAGAATGTTAA

Protein Analysis

293

Amino Acids

33.54

Weight (kDa)

9.03

Isoelectric Point (pI)

47.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 40 - 222 9.9e-58 Glycosyl hydrolases family 16
XET_C PF06955 253 - 293 1.3e-12 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014989)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44990
fragaria_vesca FvH4_3g30850
malus_domestica MD11G1146700.v1.1
prunus_persica Prupe.6G110300_v2.0.a1
pyrus_communis pycom11g11970
rosa_chinensis RchiOBHm_Chr5g0057091
rosa_laevigata RLG00000035150
rosa_multiflora Rmu_co8466263.1_g000001 Rmu_sc0001334.1_g000001 Rmu_sc0007667.1_g000003
rosa_roxburghii Rroxscaffold_1G00023110
rosa_rugosa Rorug05G0305700
rosa_samantha Rh5AG375000 Rh5BG386000 Rh5CG409500
rosa_wichuraiana Rw5G035300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 428
Acc16I TGCGCA 1 cut(s) 764
AccI GTMKAC 1 cut(s) 531
AccII CGCG 1 cut(s) 771
AciI CCGC 6 cut(s) 707, 719, 743, 769, 771, 794
AclI AACGTT 1 cut(s) 64
AclWI GGATC 3 cut(s) 613, 656, 839
AcsI RAATTY 2 cut(s) 125, 449
AfaI GTAC 5 cut(s) 102, 133, 540, 550, 689
AfiI CCNNNNNNNGG 3 cut(s) 50, 77, 340
AgeI ACCGGT 1 cut(s) 231
AgsI TTSAA 2 cut(s) 214, 392
AjnI CCWGG 3 cut(s) 268, 325, 370
AluBI AGCT 6 cut(s) 26, 262, 572, 703, 726, 732
AluI AGCT 6 cut(s) 26, 262, 572, 703, 726, 732
Alw26I GTCTC 4 cut(s) 15, 222, 324, 849
AlwI GGATC 3 cut(s) 613, 656, 839
Ama87I CYCGRG 2 cut(s) 88, 96
AoxI GGCC 1 cut(s) 630
ApeKI GCWGC 1 cut(s) 784
ApoI RAATTY 2 cut(s) 125, 449
AsiGI ACCGGT 1 cut(s) 231
Asp700I GAANNNNTTC 1 cut(s) 401
AspLEI GCGC 1 cut(s) 765
AspS9I GGNCC 1 cut(s) 630
AsuC2I CCSGG 1 cut(s) 78
AsuHPI GGTGA 3 cut(s) 66, 102, 476
AsuNHI GCTAGC 1 cut(s) 703
AvaI CYCGRG 2 cut(s) 88, 96
BaeGI GKGCMC 2 cut(s) 44, 811
BanII GRGCYC 1 cut(s) 146
BbsI GAAGAC 1 cut(s) 394
BbvI GCAGC 1 cut(s) 796
BccI CCATC 2 cut(s) 754, 784
BciT130I CCWGG 3 cut(s) 270, 327, 372
BciVI GTATCC 2 cut(s) 74, 283
BcnI CCSGG 1 cut(s) 78
BcoDI GTCTC 4 cut(s) 15, 222, 324, 849
BfaI CTAG 5 cut(s) 23, 45, 359, 704, 729
BfuI GTATCC 2 cut(s) 74, 283
BisI GCNGC 2 cut(s) 785, 795
BlsI GCNGC 2 cut(s) 786, 796
Bme1390I CCNGG 4 cut(s) 78, 270, 327, 372
BmeT110I CYCGRG 2 cut(s) 88, 96
BmgT120I GGNCC 1 cut(s) 630
BmiI GGNNCC 1 cut(s) 506
BmrFI CCNGG 4 cut(s) 78, 270, 327, 372
BmrI ACTGGG 2 cut(s) 210, 637
BmsI GCATC 4 cut(s) 331, 607, 629, 701
BmtI GCTAGC 1 cut(s) 707
BmuI ACTGGG 2 cut(s) 210, 637
BpiI GAAGAC 1 cut(s) 394
BpmI CTGGAG 2 cut(s) 303, 348
BpuEI CTTGAG 1 cut(s) 459
BpuMI CCSGG 1 cut(s) 78
Bsa29I ATCGAT 2 cut(s) 608, 664
BsaAI YACGTR 1 cut(s) 548
BsaI GGTCTC 2 cut(s) 324, 849
BsaJI CCNNGG 2 cut(s) 371, 769
BsaWI WCCGGW 1 cut(s) 231
Bsc4I CCNNNNNNNGG 3 cut(s) 50, 77, 340
Bse118I RCCGGY 2 cut(s) 231, 780
Bse1I ACTGG 2 cut(s) 205, 632
BseBI CCWGG 3 cut(s) 270, 327, 372
BseCI ATCGAT 2 cut(s) 608, 664
BseDI CCNNGG 2 cut(s) 371, 769
BseGI GGATG 4 cut(s) 293, 322, 622, 716
BseLI CCNNNNNNNGG 3 cut(s) 50, 77, 340
BseNI ACTGG 2 cut(s) 205, 632
BseSI GKGCMC 2 cut(s) 44, 811
BseXI GCAGC 1 cut(s) 796
BsgI GTGCAG 1 cut(s) 738
Bsh1236I CGCG 1 cut(s) 771
Bsh1285I CGRYCG 2 cut(s) 236, 424
BshFI GGCC 1 cut(s) 632
BshTI ACCGGT 1 cut(s) 231
BshVI ATCGAT 2 cut(s) 608, 664
BsiEI CGRYCG 2 cut(s) 236, 424
BsiHKCI CYCGRG 2 cut(s) 88, 96
BsiSI CCGG 3 cut(s) 77, 232, 781
BslFI GGGAC 1 cut(s) 376
BslI CCNNNNNNNGG 3 cut(s) 50, 77, 340
BsmAI GTCTC 4 cut(s) 15, 222, 324, 849
BsmFI GGGAC 1 cut(s) 376
BsnI GGCC 1 cut(s) 632
Bso31I GGTCTC 2 cut(s) 324, 849
BsoBI CYCGRG 2 cut(s) 88, 96
Bsp1286I GDGCHC 3 cut(s) 44, 146, 811
Bsp143I GATC 4 cut(s) 118, 605, 648, 844
BspACI CCGC 6 cut(s) 707, 719, 743, 769, 771, 794
BspANI GGCC 1 cut(s) 632
BspDI ATCGAT 2 cut(s) 608, 664
BspFNI CGCG 1 cut(s) 771
BspLI GGNNCC 1 cut(s) 506
BspOI GCTAGC 1 cut(s) 707
BspPI GGATC 3 cut(s) 613, 656, 839
BspTNI GGTCTC 2 cut(s) 324, 849
BsrFI RCCGGY 2 cut(s) 231, 780
BsrI ACTGG 2 cut(s) 205, 632
BssAI RCCGGY 2 cut(s) 231, 780
BssECI CCNNGG 2 cut(s) 371, 769
BssMI GATC 4 cut(s) 118, 605, 648, 844
Bst2UI CCWGG 3 cut(s) 270, 327, 372
Bst6I CTCTTC 2 cut(s) 225, 433
BstBAI YACGTR 1 cut(s) 548
BstC8I GCNNGC 3 cut(s) 705, 753, 782
BstDEI CTNAG 2 cut(s) 31, 684
BstDSI CCRYGG 1 cut(s) 769
BstF5I GGATG 4 cut(s) 293, 322, 622, 716
BstFNI CGCG 1 cut(s) 771
BstHHI GCGC 1 cut(s) 765
BstKTI GATC 4 cut(s) 121, 608, 651, 847
BstMAI GTCTC 4 cut(s) 15, 222, 324, 849
BstMBI GATC 4 cut(s) 118, 605, 648, 844
BstMCI CGRYCG 2 cut(s) 236, 424
BstMWI GCNNNNNNNGC 2 cut(s) 376, 777
BstNI CCWGG 3 cut(s) 270, 327, 372
BstSCI CCNGG 4 cut(s) 76, 268, 325, 370
BstSLI GKGCMC 2 cut(s) 44, 811
BstSNI TACGTA 1 cut(s) 548
BstUI CGCG 1 cut(s) 771
BstV1I GCAGC 1 cut(s) 796
BstV2I GAAGAC 1 cut(s) 394
Bsu15I ATCGAT 2 cut(s) 608, 664
BsuI GTATCC 2 cut(s) 74, 283
BsuRI GGCC 1 cut(s) 632
BsuTUI ATCGAT 2 cut(s) 608, 664
BtgI CCRYGG 1 cut(s) 769
BtgZI GCGATG 1 cut(s) 732
BtsCI GGATG 4 cut(s) 293, 322, 622, 716
BtsI GCAGTG 1 cut(s) 276
BtsIMutI CAGTG 2 cut(s) 198, 276
Cac8I GCNNGC 3 cut(s) 705, 753, 782
CfoI GCGC 1 cut(s) 765
Cfr10I RCCGGY 2 cut(s) 231, 780
Cfr13I GGNCC 1 cut(s) 630
Cfr42I CCGCGG 1 cut(s) 772
ClaI ATCGAT 2 cut(s) 608, 664
Csp6I GTAC 5 cut(s) 101, 132, 539, 549, 688
CspAI ACCGGT 1 cut(s) 231
CviAII CATG 4 cut(s) 735, 760, 804, 842
CviQI GTAC 5 cut(s) 101, 132, 539, 549, 688
DdeI CTNAG 2 cut(s) 31, 684
DpnI GATC 4 cut(s) 120, 607, 650, 846
DpnII GATC 4 cut(s) 118, 605, 648, 844
DrdI GACNNNNNNGTC 1 cut(s) 428
DseDI GACNNNNNNGTC 1 cut(s) 428
Eam1104I CTCTTC 2 cut(s) 225, 433
EarI CTCTTC 2 cut(s) 225, 433
Eco105I TACGTA 1 cut(s) 548
Eco24I GRGCYC 1 cut(s) 146
Eco31I GGTCTC 2 cut(s) 324, 849
Eco32I GATATC 1 cut(s) 349
Eco88I CYCGRG 2 cut(s) 88, 96
EcoRI GAATTC 1 cut(s) 125
EcoRII CCWGG 3 cut(s) 268, 325, 370
EcoRV GATATC 1 cut(s) 349
EcoT22I ATGCAT 1 cut(s) 622
EcoT38I GRGCYC 1 cut(s) 146
FaeI CATG 4 cut(s) 738, 763, 807, 845
FaqI GGGAC 1 cut(s) 376
FatI CATG 4 cut(s) 734, 759, 803, 841
FauI CCCGC 2 cut(s) 700, 764
FblI GTMKAC 1 cut(s) 531
Fnu4HI GCNGC 2 cut(s) 785, 795
FokI GGATG 4 cut(s) 280, 309, 629, 723
FriOI GRGCYC 1 cut(s) 146
Fsp4HI GCNGC 2 cut(s) 785, 795
FspBI CTAG 5 cut(s) 23, 45, 359, 704, 729
FspI TGCGCA 1 cut(s) 764
GlaI GCGC 1 cut(s) 764
GluI GCNGC 2 cut(s) 785, 795
GsuI CTGGAG 2 cut(s) 303, 348
HaeIII GGCC 1 cut(s) 632
HapII CCGG 3 cut(s) 77, 232, 781
HhaI GCGC 1 cut(s) 765
Hin1II CATG 4 cut(s) 738, 763, 807, 845
Hin6I GCGC 1 cut(s) 763
HinP1I GCGC 1 cut(s) 763
HpaII CCGG 3 cut(s) 77, 232, 781
HphI GGTGA 3 cut(s) 66, 102, 476
Hpy166II GTNNAC 2 cut(s) 532, 601
Hpy188I TCNGA 2 cut(s) 625, 849
Hpy188III TCNNGA 3 cut(s) 96, 122, 476
Hpy8I GTNNAC 2 cut(s) 532, 601
HpyAV CCTTC 3 cut(s) 81, 398, 639
HpyCH4IV ACGT 4 cut(s) 64, 157, 171, 547
HpyCH4V TGCA 4 cut(s) 620, 714, 738, 755
HpyF10VI GCNNNNNNNGC 2 cut(s) 376, 777
HpyF3I CTNAG 2 cut(s) 31, 684
HpySE526I ACGT 4 cut(s) 64, 157, 171, 547
Hsp92II CATG 4 cut(s) 738, 763, 807, 845
HspAI GCGC 1 cut(s) 763
KroI GCCGGC 1 cut(s) 780
KroNI GCCGGC 1 cut(s) 782
KspI CCGCGG 1 cut(s) 772
Kzo9I GATC 4 cut(s) 118, 605, 648, 844
LmnI GCTCC 1 cut(s) 267
Lsp1109I GCAGC 1 cut(s) 796
LweI GCATC 4 cut(s) 331, 607, 629, 701
MaeI CTAG 5 cut(s) 23, 45, 359, 704, 729
MaeII ACGT 4 cut(s) 64, 157, 171, 547
MaeIII GTNAC 2 cut(s) 53, 175
MalI GATC 4 cut(s) 120, 607, 650, 846
MboI GATC 4 cut(s) 118, 605, 648, 844
MboII GAAGA 3 cut(s) 212, 394, 450
MhlI GDGCHC 3 cut(s) 44, 146, 811
MluCI AATT 4 cut(s) 125, 449, 495, 516
MmeI TCCRAC 1 cut(s) 289
MnlI CCTC 3 cut(s) 308, 334, 807
Mph1103I ATGCAT 1 cut(s) 622
MroNI GCCGGC 1 cut(s) 780
MroXI GAANNNNTTC 1 cut(s) 401
MseI TTAA 3 cut(s) 258, 699, 880
MslI CAYNNNNRTG 2 cut(s) 802, 874
MspA1I CMGCKG 2 cut(s) 719, 771
MspI CCGG 3 cut(s) 77, 232, 781
MspR9I CCNGG 4 cut(s) 78, 270, 327, 372
MvaI CCWGG 3 cut(s) 270, 327, 372
MvnI CGCG 1 cut(s) 771
MwoI GCNNNNNNNGC 2 cut(s) 376, 777
NaeI GCCGGC 1 cut(s) 782
NciI CCSGG 1 cut(s) 78
NdeII GATC 4 cut(s) 118, 605, 648, 844
NgoMIV GCCGGC 1 cut(s) 780
NheI GCTAGC 1 cut(s) 703
NlaIII CATG 4 cut(s) 738, 763, 807, 845
NlaIV GGNNCC 1 cut(s) 506
NmuCI GTSAC 2 cut(s) 53, 175
NsbI TGCGCA 1 cut(s) 764
NsiI ATGCAT 1 cut(s) 622
PcsI WCGNNNNNNNCGW 2 cut(s) 127, 342
PdiI GCCGGC 1 cut(s) 782
PdmI GAANNNNTTC 1 cut(s) 401
PflFI GACNNNGTC 1 cut(s) 173
PfoI TCCNGGA 1 cut(s) 325
PinAI ACCGGT 1 cut(s) 231
PkrI GCNGC 2 cut(s) 786, 796
Ppu21I YACGTR 1 cut(s) 548
Psp1406I AACGTT 1 cut(s) 64
Psp6I CCWGG 3 cut(s) 268, 325, 370
PspGI CCWGG 3 cut(s) 268, 325, 370
PspN4I GGNNCC 1 cut(s) 506
PspPI GGNCC 1 cut(s) 630
PsyI GACNNNGTC 1 cut(s) 173
RsaI GTAC 5 cut(s) 102, 133, 540, 550, 689
RsaNI GTAC 5 cut(s) 101, 132, 539, 549, 688
RseI CAYNNNNRTG 2 cut(s) 802, 874
SacII CCGCGG 1 cut(s) 772
SaqAI TTAA 3 cut(s) 258, 699, 880
SatI GCNGC 2 cut(s) 785, 795
Sau3AI GATC 4 cut(s) 118, 605, 648, 844
Sau96I GGNCC 1 cut(s) 630
ScrFI CCNGG 4 cut(s) 78, 270, 327, 372
SduI GDGCHC 3 cut(s) 44, 146, 811
SfaNI GCATC 4 cut(s) 331, 607, 629, 701
Sfr303I CCGCGG 1 cut(s) 772
SgrBI CCGCGG 1 cut(s) 772
SmiMI CAYNNNNRTG 2 cut(s) 802, 874
SmlI CTYRAG 1 cut(s) 474
SmoI CTYRAG 1 cut(s) 474
SnaBI TACGTA 1 cut(s) 548
Sse9I AATT 4 cut(s) 125, 449, 495, 516
SsiI CCGC 6 cut(s) 707, 719, 743, 769, 771, 794
SspMI CTAG 5 cut(s) 23, 45, 359, 704, 729
StyD4I CCNGG 4 cut(s) 76, 268, 325, 370
TaiI ACGT 4 cut(s) 67, 160, 174, 550
TaqI TCGA 5 cut(s) 189, 345, 351, 608, 664
TaqII GACCGA 2 cut(s) 438, 604
TasI AATT 4 cut(s) 125, 449, 495, 516
TatI WGTACW 1 cut(s) 687
TauI GCSGC 1 cut(s) 797
Tru1I TTAA 3 cut(s) 258, 699, 880
Tru9I TTAA 3 cut(s) 258, 699, 880
TscAI CASTG 2 cut(s) 205, 283
TseFI GTSAC 2 cut(s) 53, 175
TseI GCWGC 1 cut(s) 784
Tsp45I GTSAC 2 cut(s) 53, 175
TspDTI ATGAA 2 cut(s) 461, 582
TspGWI ACGGA 2 cut(s) 119, 618
TspRI CASTG 2 cut(s) 205, 283
Tth111I GACNNNGTC 1 cut(s) 173
XapI RAATTY 2 cut(s) 125, 449
XcmI CCANNNNNNNNNTGG 1 cut(s) 323
XmiI GTMKAC 1 cut(s) 531
XmnI GAANNNNTTC 1 cut(s) 401
XspI CTAG 5 cut(s) 23, 45, 359, 704, 729
Zsp2I ATGCAT 1 cut(s) 622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.