AT3G49140

protein At3g49140-like isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0006155
Physical Location & Seq
Reverse (-)
18211863 .. 18215743
3881 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G49140.1

Sequence Viewer

Length: 1500 bp
ATGATTGAGTCGGTTATGGCCGTTCGTCTCTCCACCGGATTTTGCTCGTCGACGGCGTTACTCCAGTATCGTACAGCACCGAGCTCGGAGGAGGGAGGAAACTGCTTCCATTATGCGTCCCGCCGCGTTTTTCAGCCTCAACGTATCCACCATATTGATGGGAGTGGGTTTCTGAAGTACAACAGTGACTATATAACAAGAAAACACCTACGGAAGAATCGAACTCAAGCCACAGCTGAGTATGTAGATTCAGCTTCAGATCCAGAAAAGCAAACTGGCAAGTCAAGGTATCATCCTTCAGAAGAAATCAGAGCCTCTTTGCCACAGAATGATGGAGATTCCAGGCTTTCACCTGCAGAAACTACTAGAACCATCATTGAGGTGAACAATAAAGGAACCCTGATGCTTACAGGTTCTATTGGTGATGGAGTTCATGAGAATATTCTCTGGCCAGATATACCTTATATAACAGATCAAAATGGAAATTTATACTTTCAAGTAAAGGAGGATGAGGACGTCATGCAGTCCGTTACTTCTGAAAATAATTATGTGCAAGTTATAGTTGGTTTTGATACGATGGAGATGATCAAGGAAATGGAGCTGATGGGACTTTCTGATAGTGACTTTGAAACTGAAGATGATGAAAGTGGAGATGATGACAGTGAGGATACAGGAGAAGACGAAGATGAGGAGGAATGGGTTGCTATTTTAGAGGATGAAGATGAAGACGATGATGATGATGATGATGATGATGAGGATGATGATGATTCTGATTCTGATGAATCGCTGGGAGACTGGGCAAATTTGGAAACAATGAGGAGTTGTCATCCCATGTTTTTTGCAAAGAGAATGACTGAGGTTGCTTCAAATGATCCTGTTGATTGGATGGATCAGCCGTCTGCTGGCCTTGCTATCCAGGGACTTTTGAGTCATATCTTAGTGGAAGATTATTCAGATATCCAAAAGAAACTTGCTGATAGCAATTCTACAACTAACGGAAACAAGGATGCAGAAAACTTAGTGGACAAGCTTGAAGACAATAGTAAGGCAGGTGGTGACGAATCTGAGATAGATTCATCACAGGATGAAAAAGCAAGAAATGTGGTGGCGTTTTACAAGCTGGAGATGATAAGAATTCAGCTTATCACAGCACAAGGGGATCAGACTGAAGTTGAGGTGGAAGATGTCAGAAAAGCACAACCTGATGCTATTGCTCATGCATCAGCTGAAATCATAAGCCGTTTAGAAGAATCTGGTGATAAAATAACTGAAGCACTCAAATCTCTGTGTTGGAGACATAACAGTATTCAAGCAGAGGAAGTGAAGCTGATTGGGATAGATTCCCTAGGTTTTGACCTCAGGCTTTGCGCAGGAGCAAAGATTGAGTCATTGCGATTTGCATTCTCGACAAGGGCAACATCAGAAGAAAACGCAGAGGGACAAATAAGAAAACTATTATTCCCGAAAACGAACCAGTCGACACAACCAAAACCAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

499

Amino Acids

55.87

Weight (kDa)

4.29

Isoelectric Point (pI)

50.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 361, 1040
AasI GACNNNNNNGTC 1 cut(s) 927
AatII GACGTC 1 cut(s) 519
Acc16I TGCGCA 1 cut(s) 1369
Acc36I ACCTGC 2 cut(s) 361, 1040
AccI GTMKAC 2 cut(s) 50, 1478
AccII CGCG 1 cut(s) 126
AciI CCGC 2 cut(s) 121, 124
AclWI GGATC 4 cut(s) 254, 866, 897, 1167
AcoI YGGCCR 2 cut(s) 18, 449
AcsI RAATTY 3 cut(s) 484, 802, 1134
AcuI CTGAAG 6 cut(s) 194, 240, 282, 654, 1188, 1290
AcyI GRCGYC 1 cut(s) 516
AfaI GTAC 2 cut(s) 73, 179
AfiI CCNNNNNNNGG 1 cut(s) 902
AgsI TTSAA 5 cut(s) 497, 629, 867, 1034, 1310
AjnI CCWGG 2 cut(s) 341, 915
AluBI AGCT 9 cut(s) 84, 236, 254, 601, 1030, 1120, 1141, 1226, 1327
AluI AGCT 9 cut(s) 84, 236, 254, 601, 1030, 1120, 1141, 1226, 1327
Alw21I GWGCWC 1 cut(s) 86
Alw26I GTCTC 3 cut(s) 32, 786, 1288
AlwI GGATC 4 cut(s) 254, 866, 897, 1167
AoxI GGCC 3 cut(s) 18, 449, 904
ApoI RAATTY 3 cut(s) 484, 802, 1134
ArsI GACNNNNNNTTYG 2 cut(s) 1370, 1402
AspA2I CCTAGG 1 cut(s) 1345
AspLEI GCGC 1 cut(s) 1370
AsuHPI GGTGA 5 cut(s) 342, 394, 434, 1067, 1268
AvrII CCTAGG 1 cut(s) 1345
AxyI CCTNAGG 1 cut(s) 1358
BaeI ACNNNNGTAYC 2 cut(s) 50, 83
BalI TGGCCA 1 cut(s) 451
BanII GRGCYC 1 cut(s) 86
BbsI GAAGAC 3 cut(s) 684, 732, 1041
Bbv12I GWGCWC 1 cut(s) 86
BccI CCATC 7 cut(s) 152, 326, 380, 419, 571, 598, 880
BceAI ACGGC 4 cut(s) 5, 69, 880, 1224
BcgI CGANNNNNNTGC 2 cut(s) 66, 100
BciT130I CCWGG 2 cut(s) 343, 917
BciVI GTATCC 2 cut(s) 155, 661
BclI TGATCA 1 cut(s) 585
BcoDI GTCTC 3 cut(s) 32, 786, 1288
BfaI CTAG 2 cut(s) 366, 1346
BfmI CTRYAG 1 cut(s) 354
BfuAI ACCTGC 2 cut(s) 361, 1040
BfuI GTATCC 2 cut(s) 155, 661
BisI GCNGC 1 cut(s) 124
BlnI CCTAGG 1 cut(s) 1345
BlsI GCNGC 1 cut(s) 125
Bme1390I CCNGG 2 cut(s) 343, 917
BmiI GGNNCC 1 cut(s) 397
BmrFI CCNGG 2 cut(s) 343, 917
BmrI ACTGGG 1 cut(s) 805
BmsI GCATC 4 cut(s) 393, 997, 1195, 1229
BmuI ACTGGG 1 cut(s) 805
BpiI GAAGAC 3 cut(s) 684, 732, 1041
BpmI CTGGAG 2 cut(s) 47, 1142
BpuEI CTTGAG 1 cut(s) 210
BsaHI GRCGYC 1 cut(s) 516
BsaJI CCNNGG 2 cut(s) 916, 1345
BsaWI WCCGGW 1 cut(s) 35
Bsc4I CCNNNNNNNGG 1 cut(s) 902
Bse1I ACTGG 4 cut(s) 64, 280, 800, 1474
Bse21I CCTNAGG 1 cut(s) 1358
Bse3DI GCAATG 1 cut(s) 1388
BseBI CCWGG 2 cut(s) 343, 917
BseDI CCNNGG 2 cut(s) 916, 1345
BseGI GGATG 8 cut(s) 292, 514, 721, 763, 826, 891, 1012, 1090
BseLI CCNNNNNNNGG 1 cut(s) 902
BseMI GCAATG 1 cut(s) 1388
BseMII CTCAG 4 cut(s) 228, 846, 1056, 1372
BseNI ACTGG 4 cut(s) 64, 280, 800, 1474
BseRI GAGGAG 3 cut(s) 104, 704, 832
BseYI CCCAGC 1 cut(s) 787
Bsh1236I CGCG 1 cut(s) 126
BshFI GGCC 3 cut(s) 20, 451, 906
BsiHKAI GWGCWC 1 cut(s) 86
BsiSI CCGG 1 cut(s) 36
BslFI GGGAC 4 cut(s) 103, 621, 933, 1452
BslI CCNNNNNNNGG 1 cut(s) 902
BsmAI GTCTC 3 cut(s) 32, 786, 1288
BsmBI CGTCTC 1 cut(s) 32
BsmFI GGGAC 4 cut(s) 103, 621, 933, 1452
BsmI GAATGC 1 cut(s) 1400
BsnI GGCC 3 cut(s) 20, 451, 906
Bsp1286I GDGCHC 1 cut(s) 86
Bsp143I GATC 6 cut(s) 259, 472, 585, 871, 889, 1159
BspACI CCGC 2 cut(s) 121, 124
BspANI GGCC 3 cut(s) 20, 451, 906
BspCNI CTCAG 4 cut(s) 229, 847, 1057, 1371
BspFNI CGCG 1 cut(s) 126
BspHI TCATGA 1 cut(s) 433
BspLI GGNNCC 1 cut(s) 397
BspMAI CTGCAG 1 cut(s) 358
BspMI ACCTGC 2 cut(s) 361, 1040
BspPI GGATC 4 cut(s) 254, 866, 897, 1167
BsrDI GCAATG 1 cut(s) 1388
BsrI ACTGG 4 cut(s) 64, 280, 800, 1474
BssECI CCNNGG 2 cut(s) 916, 1345
BssMI GATC 6 cut(s) 259, 472, 585, 871, 889, 1159
BssNI GRCGYC 1 cut(s) 516
BssT1I CCWWGG 1 cut(s) 1345
Bst2UI CCWGG 2 cut(s) 343, 917
Bst4CI ACNGT 3 cut(s) 185, 662, 1304
BstACI GRCGYC 1 cut(s) 516
BstC8I GCNNGC 1 cut(s) 904
BstDEI CTNAG 6 cut(s) 237, 855, 937, 1018, 1065, 1358
BstF5I GGATG 8 cut(s) 292, 514, 721, 763, 826, 891, 1012, 1090
BstFNI CGCG 1 cut(s) 126
BstHHI GCGC 1 cut(s) 1370
BstKTI GATC 6 cut(s) 262, 475, 588, 874, 892, 1162
BstMAI GTCTC 3 cut(s) 32, 786, 1288
BstMBI GATC 6 cut(s) 259, 472, 585, 871, 889, 1159
BstMWI GCNNNNNNNGC 1 cut(s) 908
BstNI CCWGG 2 cut(s) 343, 917
BstSCI CCNGG 2 cut(s) 341, 915
BstSFI CTRYAG 1 cut(s) 354
BstUI CGCG 1 cut(s) 126
BstV2I GAAGAC 3 cut(s) 684, 732, 1041
BstX2I RGATCY 1 cut(s) 259
BstXI CCANNNNNNTGG 1 cut(s) 158
BstYI RGATCY 1 cut(s) 259
Bsu36I CCTNAGG 1 cut(s) 1358
BsuI GTATCC 2 cut(s) 155, 661
BsuRI GGCC 3 cut(s) 20, 451, 906
BtsCI GGATG 8 cut(s) 292, 514, 721, 763, 826, 891, 1012, 1090
BtsIMutI CAGTG 2 cut(s) 190, 667
BveI ACCTGC 2 cut(s) 361, 1040
Cac8I GCNNGC 1 cut(s) 904
CciI TCATGA 1 cut(s) 433
CfoI GCGC 1 cut(s) 1370
CseI GACGC 1 cut(s) 105
Csp6I GTAC 2 cut(s) 72, 178
CspCI CAANNNNNGTGG 2 cut(s) 1083, 1118
CviAII CATG 4 cut(s) 434, 520, 832, 1217
CviQI GTAC 2 cut(s) 72, 178
DdeI CTNAG 6 cut(s) 237, 855, 937, 1018, 1065, 1358
DpnI GATC 6 cut(s) 261, 474, 587, 873, 891, 1161
DpnII GATC 6 cut(s) 259, 472, 585, 871, 889, 1159
DrdI GACNNNNNNGTC 1 cut(s) 927
DseDI GACNNNNNNGTC 1 cut(s) 927
EaeI YGGCCR 2 cut(s) 18, 449
Ecl136II GAGCTC 1 cut(s) 84
Eco130I CCWWGG 1 cut(s) 1345
Eco24I GRGCYC 1 cut(s) 86
Eco32I GATATC 1 cut(s) 958
Eco53kI GAGCTC 1 cut(s) 84
Eco57I CTGAAG 6 cut(s) 194, 240, 282, 654, 1188, 1290
Eco81I CCTNAGG 1 cut(s) 1358
EcoICRI GAGCTC 1 cut(s) 84
EcoRI GAATTC 1 cut(s) 1134
EcoRII CCWGG 2 cut(s) 341, 915
EcoRV GATATC 1 cut(s) 958
EcoT14I CCWWGG 1 cut(s) 1345
EcoT22I ATGCAT 1 cut(s) 1222
EcoT38I GRGCYC 1 cut(s) 86
ErhI CCWWGG 1 cut(s) 1345
Esp3I CGTCTC 1 cut(s) 32
FaeI CATG 4 cut(s) 437, 523, 835, 1220
FaqI GGGAC 4 cut(s) 103, 621, 933, 1452
FatI CATG 4 cut(s) 433, 519, 831, 1216
FauI CCCGC 1 cut(s) 128
FbaI TGATCA 1 cut(s) 585
FblI GTMKAC 2 cut(s) 50, 1478
Fnu4HI GCNGC 1 cut(s) 124
FokI GGATG 8 cut(s) 279, 521, 728, 770, 813, 898, 1019, 1097
FriOI GRGCYC 1 cut(s) 86
Fsp4HI GCNGC 1 cut(s) 124
FspBI CTAG 2 cut(s) 366, 1346
FspI TGCGCA 1 cut(s) 1369
GlaI GCGC 1 cut(s) 1369
GluI GCNGC 1 cut(s) 124
GsaI CCCAGC 1 cut(s) 791
GsuI CTGGAG 2 cut(s) 47, 1142
HaeIII GGCC 3 cut(s) 20, 451, 906
HapII CCGG 1 cut(s) 36
HgaI GACGC 1 cut(s) 105
HhaI GCGC 1 cut(s) 1370
Hin1I GRCGYC 1 cut(s) 516
Hin1II CATG 4 cut(s) 437, 523, 835, 1220
Hin6I GCGC 1 cut(s) 1368
HinP1I GCGC 1 cut(s) 1368
HincII GTYRAC 2 cut(s) 51, 1479
HindII GTYRAC 2 cut(s) 51, 1479
HindIII AAGCTT 1 cut(s) 1028
HpaII CCGG 1 cut(s) 36
HphI GGTGA 5 cut(s) 342, 394, 434, 1067, 1268
Hpy166II GTNNAC 4 cut(s) 51, 385, 1024, 1479
Hpy188III TCNNGA 4 cut(s) 263, 434, 1405, 1462
Hpy8I GTNNAC 4 cut(s) 51, 385, 1024, 1479
Hpy99I CGWCG 2 cut(s) 52, 55
HpyAV CCTTC 1 cut(s) 306
HpyCH4III ACNGT 3 cut(s) 185, 662, 1304
HpyCH4IV ACGT 2 cut(s) 142, 516
HpyCH4V TGCA 7 cut(s) 356, 523, 553, 842, 1010, 1220, 1400
HpyF10VI GCNNNNNNNGC 1 cut(s) 908
HpyF3I CTNAG 6 cut(s) 237, 855, 937, 1018, 1065, 1358
HpySE526I ACGT 2 cut(s) 142, 516
Hsp92I GRCGYC 1 cut(s) 516
Hsp92II CATG 4 cut(s) 437, 523, 835, 1220
HspAI GCGC 1 cut(s) 1368
Ksp22I TGATCA 1 cut(s) 585
Kzo9I GATC 6 cut(s) 259, 472, 585, 871, 889, 1159
LmnI GCTCC 2 cut(s) 598, 1373
LweI GCATC 4 cut(s) 393, 997, 1195, 1229
MaeI CTAG 2 cut(s) 366, 1346
MaeII ACGT 2 cut(s) 142, 516
MaeIII GTNAC 5 cut(s) 57, 185, 529, 620, 1055
MalI GATC 6 cut(s) 261, 474, 587, 873, 891, 1161
MboI GATC 6 cut(s) 259, 472, 585, 871, 889, 1159
MflI RGATCY 1 cut(s) 259
MhlI GDGCHC 1 cut(s) 86
MlsI TGGCCA 1 cut(s) 451
MluCI AATT 5 cut(s) 484, 544, 802, 982, 1134
MluNI TGGCCA 1 cut(s) 451
MlyI GAGTC 3 cut(s) 17, 937, 1394
MmeI TCCRAC 1 cut(s) 1271
Mox20I TGGCCA 1 cut(s) 451
Mph1103I ATGCAT 1 cut(s) 1222
MscI TGGCCA 1 cut(s) 451
MslI CAYNNNNRTG 2 cut(s) 156, 380
Msp20I TGGCCA 1 cut(s) 451
MspA1I CMGCKG 2 cut(s) 236, 1226
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 2 cut(s) 343, 917
Mva1269I GAATGC 1 cut(s) 1400
MvaI CCWGG 2 cut(s) 343, 917
MvnI CGCG 1 cut(s) 126
MwoI GCNNNNNNNGC 1 cut(s) 908
NdeII GATC 6 cut(s) 259, 472, 585, 871, 889, 1159
NlaIII CATG 4 cut(s) 437, 523, 835, 1220
NlaIV GGNNCC 1 cut(s) 397
NmuCI GTSAC 3 cut(s) 185, 620, 1055
NsbI TGCGCA 1 cut(s) 1369
NsiI ATGCAT 1 cut(s) 1222
PagI TCATGA 1 cut(s) 433
PaqCI CACCTGC 2 cut(s) 361, 1040
PcsI WCGNNNNNNNCGW 3 cut(s) 53, 217, 1475
PctI GAATGC 1 cut(s) 1400
PkrI GCNGC 1 cut(s) 125
PleI GAGTC 3 cut(s) 16, 936, 1393
PpsI GAGTC 3 cut(s) 16, 936, 1393
Psp124BI GAGCTC 1 cut(s) 86
Psp6I CCWGG 2 cut(s) 341, 915
PspFI CCCAGC 1 cut(s) 787
PspGI CCWGG 2 cut(s) 341, 915
PspN4I GGNNCC 1 cut(s) 397
PstI CTGCAG 1 cut(s) 358
PsuI RGATCY 1 cut(s) 259
PvuII CAGCTG 2 cut(s) 236, 1226
RsaI GTAC 2 cut(s) 73, 179
RsaNI GTAC 2 cut(s) 72, 178
RseI CAYNNNNRTG 2 cut(s) 156, 380
SacI GAGCTC 1 cut(s) 86
SalI GTCGAC 2 cut(s) 49, 1477
SatI GCNGC 1 cut(s) 124
Sau3AI GATC 6 cut(s) 259, 472, 585, 871, 889, 1159
SchI GAGTC 3 cut(s) 17, 937, 1394
ScrFI CCNGG 2 cut(s) 343, 917
SduI GDGCHC 1 cut(s) 86
SfaNI GCATC 4 cut(s) 393, 997, 1195, 1229
SfcI CTRYAG 1 cut(s) 354
SgrDI CGTCGACG 1 cut(s) 49
SmiMI CAYNNNNRTG 2 cut(s) 156, 380
SmlI CTYRAG 1 cut(s) 225
SmoI CTYRAG 1 cut(s) 225
Sse9I AATT 5 cut(s) 484, 544, 802, 982, 1134
SsiI CCGC 2 cut(s) 121, 124
SspI AATATT 1 cut(s) 442
SspMI CTAG 2 cut(s) 366, 1346
SstI GAGCTC 1 cut(s) 86
StyD4I CCNGG 2 cut(s) 341, 915
StyI CCWWGG 1 cut(s) 1345
TaaI ACNGT 3 cut(s) 185, 662, 1304
TaiI ACGT 2 cut(s) 145, 519
TaqI TCGA 4 cut(s) 50, 220, 1406, 1478
TasI AATT 5 cut(s) 484, 544, 802, 982, 1134
TatI WGTACW 1 cut(s) 177
TauI GCSGC 1 cut(s) 126
TscAI CASTG 2 cut(s) 190, 667
TseFI GTSAC 3 cut(s) 185, 620, 1055
Tsp45I GTSAC 3 cut(s) 185, 620, 1055
TspDTI ATGAA 7 cut(s) 422, 657, 732, 738, 795, 1065, 1101
TspGWI ACGGA 3 cut(s) 226, 517, 1011
TspRI CASTG 2 cut(s) 190, 667
XapI RAATTY 3 cut(s) 484, 802, 1134
XcmI CCANNNNNNNNNTGG 1 cut(s) 155
XmaJI CCTAGG 1 cut(s) 1345
XmiI GTMKAC 2 cut(s) 50, 1478
XspI CTAG 2 cut(s) 366, 1346
ZraI GACGTC 1 cut(s) 517
Zsp2I ATGCAT 1 cut(s) 1222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.