Rmu_sc0008712.1_g000004

At3g49140-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008712.1
OG0006155
Physical Location & Seq
Forward (+)
8353 .. 11984
3632 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008712.1_g000004.1.cds

Sequence Viewer

Length: 1509 bp
atggccgtacgattcaactccgccgccgccaatgtctgctcctccaccgccgtcccttgttttcgtccacggtggagctcggaggagctgaccggagcagttcacgtcacctcttgccggcttgcgtactccggcggctggggtaggattcggagatcaaagcattatgattcggttgcgaaaaggagcaattgtgtaaagaatgggattcgagctgccacggagcagttagggcctggttcggagcctgtgaagccgaatgggaggccgcagtaccatccgtttgaggatattgctgaagcgacattggataatgttggagctgcaagacttacatctgcagaatctgctaggaccattattgaggttaatagcaaagcgacgctcatgttttcgagtctgattaatgatgaggttcatgaaaatattatgtgtcctgatttgccttatgtaactgatgaacatggaaatatatactttcaagtaaaggatggtgaagacaatgcgtctatgcaatctataacttcagagaataactttgtgcaagttattattggcttagatactatggaaatgatcaatgagatggaagtaccagaaattgattttggaattgatgagattgaaggtgaatatagtgatggtgaagatggcagtgatgaggacgacgacgacgatgaagatggtgacgatgattcggattgggtttccgttcttgatgacgaagatgaagaagatgacgatgaagatgacgagactcttggagactgggcaaagctggagactatgcgttattctcacccaatgtattttgctaagaagctgactgaggttgcttcagatgatcctatagattgggcagagcaggctccagctagtttggcaattcaggggcttctaagacctgcatatattgaagaacatacggtcataaaaaagcatatgtcggatcatgagcccaatgatgatgaaaaacaagttgggagaactgtagaagatgatttagaagagcctgataagatcaatggccatgaatcaggatcttttaagggcagtccaatccgagcggaggaatcagacaatgagaagggtgaaataccaaagaatgggactactttttacaaactggagattgttaagattcagttgttttcatcacatggacatcagagtgttgttgaagtagaagacttcgtaaaagctcagcccgatgccattgcacactcagccggtaaaattatatctcgtctgaaagcaggtggagaaaagaccacacaagctctcaagtccctttgttggagactaaagggtattcaagttgaggaggtagtactcaccactgtagattctcttggatttgacttgagggtttgctcaggaacacaagttcagacgttgcggtttgcactaaatgcacgggccacttctgagtatagtgctgagagacaacttaatgatctgctattcccgaggacacagcacacgccacagaaagtaaagcaaagttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

502

Amino Acids

55.77

Weight (kDa)

4.5

Isoelectric Point (pI)

40.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1247
Acc36I ACCTGC 2 cut(s) 913, 1247
AccB7I CCANNNNNTGG 1 cut(s) 1106
AccBSI CCGCTC 1 cut(s) 1067
AciI CCGC 8 cut(s) 21, 24, 27, 48, 135, 269, 1067, 1399
AclWI GGATC 3 cut(s) 839, 957, 1048
AcoI YGGCCR 2 cut(s) 3, 1027
AcuI CTGAAG 3 cut(s) 318, 510, 822
AfaI GTAC 5 cut(s) 9, 128, 275, 594, 1332
AfiI CCNNNNNNNGG 5 cut(s) 117, 138, 1069, 1106, 1296
AgsI TTSAA 6 cut(s) 16, 482, 626, 917, 1181, 1316
AhdI GACNNNNNGTC 1 cut(s) 505
AjiI CACGTC 1 cut(s) 106
AjnI CCWGG 1 cut(s) 235
AjuI GAANNNNNNNTTGG 4 cut(s) 591, 623, 963, 995
AloI GAACNNNNNNTCC 2 cut(s) 1371, 1403
AluBI AGCT 9 cut(s) 78, 88, 215, 323, 778, 823, 875, 1202, 1280
AluI AGCT 9 cut(s) 78, 88, 215, 323, 778, 823, 875, 1202, 1280
Alw21I GWGCWC 1 cut(s) 80
Alw26I GTCTC 5 cut(s) 749, 759, 776, 1294, 1438
AlwI GGATC 3 cut(s) 839, 957, 1048
AlwNI CAGNNNCTG 1 cut(s) 347
Ama87I CYCGRG 1 cut(s) 1468
AoxI GGCC 5 cut(s) 3, 233, 266, 1027, 1419
ApeKI GCWGC 2 cut(s) 215, 323
AseI ATTAAT 1 cut(s) 405
AspS9I GGNCC 3 cut(s) 233, 354, 1419
AsuHPI GGTGA 8 cut(s) 100, 506, 641, 656, 698, 791, 1103, 1327
AvaI CYCGRG 1 cut(s) 1468
AvaII GGWCC 1 cut(s) 354
BalI TGGCCA 1 cut(s) 1029
BanII GRGCYC 2 cut(s) 80, 960
BbsI GAAGAC 2 cut(s) 504, 1194
Bbv12I GWGCWC 1 cut(s) 80
BbvI GCAGC 2 cut(s) 202, 310
BccI CCATC 6 cut(s) 285, 485, 580, 635, 644, 677
BceAI ACGGC 1 cut(s) 35
BcgI CGANNNNNNTGC 2 cut(s) 1199, 1233
BciT130I CCWGG 1 cut(s) 237
BclI TGATCA 1 cut(s) 576
BcoDI GTCTC 5 cut(s) 749, 759, 776, 1294, 1438
BfaI CTAG 2 cut(s) 351, 876
BfmI CTRYAG 4 cut(s) 339, 849, 990, 1341
BfuAI ACCTGC 2 cut(s) 913, 1247
BisI GCNGC 6 cut(s) 24, 27, 136, 216, 269, 324
BlpI GCTNAGC 1 cut(s) 1203
BlsI GCNGC 6 cut(s) 25, 28, 137, 217, 270, 325
BmcAI AGTACT 1 cut(s) 1332
Bme1390I CCNGG 1 cut(s) 237
Bme18I GGWCC 1 cut(s) 354
BmeRI GACNNNNNGTC 1 cut(s) 505
BmeT110I CYCGRG 1 cut(s) 1468
BmgBI CACGTC 1 cut(s) 106
BmgT120I GGNCC 3 cut(s) 233, 354, 1419
BmiI GGNNCC 2 cut(s) 246, 870
BmrFI CCNGG 1 cut(s) 237
BmrI ACTGGG 1 cut(s) 778
BmsI GCATC 1 cut(s) 1201
BmuI ACTGGG 1 cut(s) 778
BpiI GAAGAC 2 cut(s) 504, 1194
BpmI CTGGAG 3 cut(s) 800, 855, 1148
Bpu10I CCTNAGC 1 cut(s) 1375
Bpu1102I GCTNAGC 1 cut(s) 1203
BpuEI CTTGAG 2 cut(s) 1268, 1384
BsaJI CCNNGG 3 cut(s) 68, 219, 1469
BsaWI WCCGGW 1 cut(s) 92
BsaXI ACNNNNNCTCC 2 cut(s) 178, 208
Bsc4I CCNNNNNNNGG 5 cut(s) 117, 138, 1069, 1106, 1296
Bse118I RCCGGY 2 cut(s) 117, 1229
Bse1I ACTGG 2 cut(s) 773, 1131
Bse3DI GCAATG 1 cut(s) 1215
BseBI CCWGG 1 cut(s) 237
BseDI CCNNGG 3 cut(s) 68, 219, 1469
BseGI GGATG 2 cut(s) 277, 496
BseLI CCNNNNNNNGG 5 cut(s) 117, 138, 1069, 1106, 1296
BseMI GCAATG 1 cut(s) 1215
BseMII CTCAG 6 cut(s) 819, 1217, 1239, 1389, 1419, 1431
BseNI ACTGG 2 cut(s) 773, 1131
BseRI GAGGAG 3 cut(s) 31, 98, 1337
BseXI GCAGC 2 cut(s) 202, 310
BseYI CCCAGC 1 cut(s) 138
BshFI GGCC 5 cut(s) 5, 235, 268, 1029, 1421
BsiHKAI GWGCWC 1 cut(s) 80
BsiHKCI CYCGRG 1 cut(s) 1468
BsiSI CCGG 4 cut(s) 93, 118, 132, 1230
BsiWI CGTACG 1 cut(s) 7
BslFI GGGAC 3 cut(s) 38, 1123, 1273
BslI CCNNNNNNNGG 5 cut(s) 117, 138, 1069, 1106, 1296
BsmAI GTCTC 5 cut(s) 749, 759, 776, 1294, 1438
BsmFI GGGAC 3 cut(s) 38, 1123, 1273
BsnI GGCC 5 cut(s) 5, 235, 268, 1029, 1421
BsoBI CYCGRG 1 cut(s) 1468
Bsp1286I GDGCHC 2 cut(s) 80, 960
Bsp143I GATC 7 cut(s) 155, 576, 844, 949, 1020, 1040, 1456
Bsp1720I GCTNAGC 1 cut(s) 1203
BspACI CCGC 8 cut(s) 21, 24, 27, 48, 135, 269, 1067, 1399
BspANI GGCC 5 cut(s) 5, 235, 268, 1029, 1421
BspCNI CTCAG 6 cut(s) 820, 1216, 1238, 1388, 1420, 1432
BspHI TCATGA 2 cut(s) 418, 952
BspLI GGNNCC 2 cut(s) 246, 870
BspMAI CTGCAG 1 cut(s) 343
BspMI ACCTGC 2 cut(s) 913, 1247
BspPI GGATC 3 cut(s) 839, 957, 1048
BspQI GCTCTTC 1 cut(s) 1002
BsrBI CCGCTC 1 cut(s) 1067
BsrDI GCAATG 1 cut(s) 1215
BsrFI RCCGGY 2 cut(s) 117, 1229
BsrI ACTGG 2 cut(s) 773, 1131
BssAI RCCGGY 2 cut(s) 117, 1229
BssECI CCNNGG 3 cut(s) 68, 219, 1469
BssMI GATC 7 cut(s) 155, 576, 844, 949, 1020, 1040, 1456
Bst2UI CCWGG 1 cut(s) 237
Bst4CI ACNGT 4 cut(s) 72, 928, 991, 1342
Bst6I CTCTTC 1 cut(s) 1002
BstAPI GCANNNNNTGC 2 cut(s) 347, 1412
BstC8I GCNNGC 3 cut(s) 119, 123, 867
BstDEI CTNAG 9 cut(s) 559, 816, 828, 899, 1203, 1225, 1375, 1428, 1440
BstDSI CCRYGG 2 cut(s) 68, 219
BstF5I GGATG 2 cut(s) 277, 496
BstKTI GATC 7 cut(s) 158, 579, 847, 952, 1023, 1043, 1459
BstMAI GTCTC 5 cut(s) 749, 759, 776, 1294, 1438
BstMBI GATC 7 cut(s) 155, 576, 844, 949, 1020, 1040, 1456
BstMWI GCNNNNNNNGC 7 cut(s) 232, 253, 347, 866, 881, 1226, 1412
BstNI CCWGG 1 cut(s) 237
BstSCI CCNGG 1 cut(s) 235
BstSFI CTRYAG 4 cut(s) 339, 849, 990, 1341
BstV1I GCAGC 2 cut(s) 202, 310
BstV2I GAAGAC 2 cut(s) 504, 1194
BstX2I RGATCY 1 cut(s) 1040
BstYI RGATCY 1 cut(s) 1040
BsuRI GGCC 5 cut(s) 5, 235, 268, 1029, 1421
BtgI CCRYGG 2 cut(s) 68, 219
BtrI CACGTC 1 cut(s) 106
BtsCI GGATG 2 cut(s) 277, 496
BtsI GCAGTG 1 cut(s) 661
BtsIMutI CAGTG 2 cut(s) 661, 1338
BveI ACCTGC 2 cut(s) 913, 1247
Cac8I GCNNGC 3 cut(s) 119, 123, 867
CaiI CAGNNNCTG 1 cut(s) 347
CciI TCATGA 2 cut(s) 418, 952
Cfr10I RCCGGY 2 cut(s) 117, 1229
Cfr13I GGNCC 3 cut(s) 233, 354, 1419
CseI GACGC 2 cut(s) 391, 495
Csp6I GTAC 5 cut(s) 8, 127, 274, 593, 1331
CviAII CATG 6 cut(s) 388, 419, 464, 953, 1031, 1160
CviQI GTAC 5 cut(s) 8, 127, 274, 593, 1331
DdeI CTNAG 9 cut(s) 559, 816, 828, 899, 1203, 1225, 1375, 1428, 1440
DpnI GATC 7 cut(s) 157, 578, 846, 951, 1022, 1042, 1458
DpnII GATC 7 cut(s) 155, 576, 844, 949, 1020, 1040, 1456
DriI GACNNNNNGTC 1 cut(s) 505
EaeI YGGCCR 2 cut(s) 3, 1027
Eam1104I CTCTTC 1 cut(s) 1002
Eam1105I GACNNNNNGTC 1 cut(s) 505
EarI CTCTTC 1 cut(s) 1002
EciI GGCGGA 1 cut(s) 10
Ecl136II GAGCTC 1 cut(s) 78
Eco24I GRGCYC 2 cut(s) 80, 960
Eco47I GGWCC 1 cut(s) 354
Eco53kI GAGCTC 1 cut(s) 78
Eco57I CTGAAG 3 cut(s) 318, 510, 822
Eco88I CYCGRG 1 cut(s) 1468
EcoICRI GAGCTC 1 cut(s) 78
EcoO109I RGGNCCY 1 cut(s) 233
EcoRII CCWGG 1 cut(s) 235
EcoT38I GRGCYC 2 cut(s) 80, 960
FaeI CATG 6 cut(s) 391, 422, 467, 956, 1034, 1163
FaqI GGGAC 3 cut(s) 38, 1123, 1273
FatI CATG 6 cut(s) 387, 418, 463, 952, 1030, 1159
FauNDI CATATG 1 cut(s) 942
FbaI TGATCA 1 cut(s) 576
Fnu4HI GCNGC 6 cut(s) 24, 27, 136, 216, 269, 324
FokI GGATG 2 cut(s) 264, 503
FriOI GRGCYC 2 cut(s) 80, 960
Fsp4HI GCNGC 6 cut(s) 24, 27, 136, 216, 269, 324
FspBI CTAG 2 cut(s) 351, 876
GluI GCNGC 6 cut(s) 24, 27, 136, 216, 269, 324
GsaI CCCAGC 1 cut(s) 142
GsuI CTGGAG 3 cut(s) 800, 855, 1148
HaeIII GGCC 5 cut(s) 5, 235, 268, 1029, 1421
HapII CCGG 4 cut(s) 93, 118, 132, 1230
HgaI GACGC 2 cut(s) 391, 495
Hin1II CATG 6 cut(s) 391, 422, 467, 956, 1034, 1163
HpaII CCGG 4 cut(s) 93, 118, 132, 1230
HphI GGTGA 8 cut(s) 100, 506, 641, 656, 698, 791, 1103, 1327
Hpy166II GTNNAC 2 cut(s) 68, 103
Hpy188III TCNNGA 7 cut(s) 419, 437, 716, 953, 1038, 1377, 1468
Hpy8I GTNNAC 2 cut(s) 68, 103
Hpy99I CGWCG 4 cut(s) 385, 671, 674, 677
HpyAV CCTTC 2 cut(s) 620, 1081
HpyCH4III ACNGT 4 cut(s) 72, 928, 991, 1342
HpyCH4IV ACGT 2 cut(s) 105, 1394
HpyCH4V TGCA 8 cut(s) 326, 341, 514, 544, 908, 1220, 1406, 1415
HpyF10VI GCNNNNNNNGC 7 cut(s) 232, 253, 347, 866, 881, 1226, 1412
HpyF3I CTNAG 9 cut(s) 559, 816, 828, 899, 1203, 1225, 1375, 1428, 1440
HpySE526I ACGT 2 cut(s) 105, 1394
Hsp92II CATG 6 cut(s) 391, 422, 467, 956, 1034, 1163
KroI GCCGGC 1 cut(s) 117
KroNI GCCGGC 1 cut(s) 119
Ksp22I TGATCA 1 cut(s) 576
Kzo9I GATC 7 cut(s) 155, 576, 844, 949, 1020, 1040, 1456
LguI GCTCTTC 1 cut(s) 1002
LmnI GCTCC 9 cut(s) 44, 75, 85, 95, 186, 223, 244, 320, 874
Lsp1109I GCAGC 2 cut(s) 202, 310
LweI GCATC 1 cut(s) 1201
MaeI CTAG 2 cut(s) 351, 876
MaeII ACGT 2 cut(s) 105, 1394
MaeIII GTNAC 3 cut(s) 106, 451, 686
MalI GATC 7 cut(s) 157, 578, 846, 951, 1022, 1042, 1458
MbiI CCGCTC 1 cut(s) 1067
MboI GATC 7 cut(s) 155, 576, 844, 949, 1020, 1040, 1456
MfeI CAATTG 1 cut(s) 190
MflI RGATCY 1 cut(s) 1040
MhlI GDGCHC 2 cut(s) 80, 960
MlsI TGGCCA 1 cut(s) 1029
MluCI AATT 5 cut(s) 190, 600, 612, 885, 1236
MluNI TGGCCA 1 cut(s) 1029
MlyI GAGTC 2 cut(s) 406, 751
MmeI TCCRAC 3 cut(s) 298, 927, 1277
Mox20I TGGCCA 1 cut(s) 1029
MroNI GCCGGC 1 cut(s) 117
MscI TGGCCA 1 cut(s) 1029
MseI TTAA 6 cut(s) 369, 405, 1047, 1137, 1452, 1507
MslI CAYNNNNRTG 1 cut(s) 1170
Msp20I TGGCCA 1 cut(s) 1029
MspI CCGG 4 cut(s) 93, 118, 132, 1230
MspR9I CCNGG 1 cut(s) 237
MunI CAATTG 1 cut(s) 190
MvaI CCWGG 1 cut(s) 237
MwoI GCNNNNNNNGC 7 cut(s) 232, 253, 347, 866, 881, 1226, 1412
NaeI GCCGGC 1 cut(s) 119
NdeI CATATG 1 cut(s) 942
NdeII GATC 7 cut(s) 155, 576, 844, 949, 1020, 1040, 1456
NgoMIV GCCGGC 1 cut(s) 117
NlaIII CATG 6 cut(s) 391, 422, 467, 956, 1034, 1163
NlaIV GGNNCC 2 cut(s) 246, 870
NmuCI GTSAC 2 cut(s) 106, 686
PagI TCATGA 2 cut(s) 418, 952
PaqCI CACCTGC 1 cut(s) 1247
PciSI GCTCTTC 1 cut(s) 1002
PcsI WCGNNNNNNNCGW 1 cut(s) 672
PdiI GCCGGC 1 cut(s) 119
Pfl23II CGTACG 1 cut(s) 7
PflMI CCANNNNNTGG 1 cut(s) 1106
PkrI GCNGC 6 cut(s) 25, 28, 137, 217, 270, 325
PleI GAGTC 2 cut(s) 405, 751
PpsI GAGTC 2 cut(s) 405, 751
PshBI ATTAAT 1 cut(s) 405
Psp124BI GAGCTC 1 cut(s) 80
Psp6I CCWGG 1 cut(s) 235
PspFI CCCAGC 1 cut(s) 138
PspGI CCWGG 1 cut(s) 235
PspLI CGTACG 1 cut(s) 7
PspN4I GGNNCC 2 cut(s) 246, 870
PspPI GGNCC 3 cut(s) 233, 354, 1419
PstI CTGCAG 1 cut(s) 343
PstNI CAGNNNCTG 1 cut(s) 347
PsuI RGATCY 1 cut(s) 1040
RsaI GTAC 5 cut(s) 9, 128, 275, 594, 1332
RsaNI GTAC 5 cut(s) 8, 127, 274, 593, 1331
RseI CAYNNNNRTG 1 cut(s) 1170
SacI GAGCTC 1 cut(s) 80
SapI GCTCTTC 1 cut(s) 1002
SaqAI TTAA 6 cut(s) 369, 405, 1047, 1137, 1452, 1507
SatI GCNGC 6 cut(s) 24, 27, 136, 216, 269, 324
Sau3AI GATC 7 cut(s) 155, 576, 844, 949, 1020, 1040, 1456
Sau96I GGNCC 3 cut(s) 233, 354, 1419
ScaI AGTACT 1 cut(s) 1332
SchI GAGTC 2 cut(s) 406, 751
ScrFI CCNGG 1 cut(s) 237
SduI GDGCHC 2 cut(s) 80, 960
SfaNI GCATC 1 cut(s) 1201
SfcI CTRYAG 4 cut(s) 339, 849, 990, 1341
SinI GGWCC 1 cut(s) 354
SmiMI CAYNNNNRTG 1 cut(s) 1170
SmlI CTYRAG 2 cut(s) 1283, 1363
SmoI CTYRAG 2 cut(s) 1283, 1363
Sse9I AATT 5 cut(s) 190, 600, 612, 885, 1236
SsiI CCGC 8 cut(s) 21, 24, 27, 48, 135, 269, 1067, 1399
SspI AATATT 1 cut(s) 427
SspMI CTAG 2 cut(s) 351, 876
SstI GAGCTC 1 cut(s) 80
StyD4I CCNGG 1 cut(s) 235
TaaI ACNGT 4 cut(s) 72, 928, 991, 1342
TaiI ACGT 2 cut(s) 108, 1397
TaqI TCGA 2 cut(s) 211, 395
TasI AATT 5 cut(s) 190, 600, 612, 885, 1236
TatI WGTACW 1 cut(s) 1330
TauI GCSGC 4 cut(s) 26, 29, 138, 271
Tru1I TTAA 6 cut(s) 369, 405, 1047, 1137, 1452, 1507
Tru9I TTAA 6 cut(s) 369, 405, 1047, 1137, 1452, 1507
TscAI CASTG 2 cut(s) 661, 1345
TseFI GTSAC 2 cut(s) 106, 686
TseI GCWGC 2 cut(s) 215, 323
Tsp45I GTSAC 2 cut(s) 106, 686
TspDTI ATGAA 9 cut(s) 407, 435, 474, 693, 744, 759, 984, 1047, 1143
TspGWI ACGGA 3 cut(s) 236, 270, 700
TspRI CASTG 2 cut(s) 661, 1345
Van91I CCANNNNNTGG 1 cut(s) 1106
VpaK11BI GGWCC 1 cut(s) 354
VspI ATTAAT 1 cut(s) 405
XspI CTAG 2 cut(s) 351, 876
ZrmI AGTACT 1 cut(s) 1332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.