pycom01g21990

At3g49140-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0006155
Physical Location & Seq
Forward (+)
19771902 .. 19773833
1932 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g21990.4

Sequence Viewer

Length: 1017 bp
ATGGCCGTACAATTCAACTCCGCCGCCACAAACCTCTGCTCCTCCACCTCCATGCCCTCCTATCGTCCGACGTGGAGCTCAGACGAACTGACCGGAGCCGTACGCTGCCGCCTTTCTTGCTCCGGCGGGTTCGCTGCGCCGTGGACTCGAATTCCACGGTCAAAGAATGGTTCGGTTGCAAAAAGGAGAAATTTGGTAAAGAATGGGATTCAGGCATCTGCCCGGCAGTTAGGGCCCGGTTCGGAGCCTGTGAAGCCGAATGAGAGGCCGGAATACCACCCATTTGAGGATATTGCCGAGTCCACATTAGAGGCTAGTGGAACTGATAGACTTACAGCTGCAGAATCTGCTAGAACCCTCATTGAGGTTAACAGCAAAGCAACACTCTTGTTTTCGAGTTTGATCAATGACGAGGTTCACGAAAATATTTTATGTCCGGATTTGCCTTATGTAACTGATGAACATGGAAATATCTACTTTCAAGTGAAGGATAGTGAAGACACTTTTCGTTCCCTAACTTCAGAGAATAACTTTGTGCAAGTTATTATTGGGTTAGATACTATGGAAATGATCAATGAGATGGAGTTACCAGAAATTGATTTTGAAATGGATGGGATTGAAGATGAAGGTAGTGAAGATGATGATGATGATGACGATGACGATGACGAGGAGGACGATGATGAAGAAGACGGTGACTATGATTCGGATTGGGTTGCTGTTCTTGAAGATGAAGACGAAGATGATCTGGATGATTCTGACAACTGGGCAAAATTGCAGACTATGCGTTACTCTCACCCAATGAATTTTGCCAAGAAGCTAAGTGAGGTTGCTTCTGATGATCCTATAGATTGGGCAGAGCAGGCTCCAGTTAGTTTGGCTATTCAGGGCCTTATAAGGCCTGCACTTATAGAAGAACATTCTGTTATCCAAAAGCATCTGTCTGATCACCAGTCTTGTAATGATGGCGTAGATGAAGTTGGGAGGACTGTAGAAGATGATTTAGAACGATCCTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

339

Amino Acids

37.71

Weight (kDa)

4.07

Isoelectric Point (pI)

46.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 893
AccIII TCCGGA 1 cut(s) 436
AciI CCGC 4 cut(s) 21, 24, 109, 126
AclWI GGATC 2 cut(s) 833, 1002
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 150, 190, 802
AcuI CTGAAG 1 cut(s) 504
AfaI GTAC 2 cut(s) 9, 102
AfiI CCNNNNNNNGG 2 cut(s) 286, 364
AgsI TTSAA 5 cut(s) 16, 482, 605, 620, 725
AjiI CACGTC 1 cut(s) 72
AluBI AGCT 3 cut(s) 78, 338, 817
AluI AGCT 3 cut(s) 78, 338, 817
Alw21I GWGCWC 1 cut(s) 80
AlwI GGATC 2 cut(s) 833, 1002
AlwNI CAGNNNCTG 1 cut(s) 347
Aor13HI TCCGGA 1 cut(s) 436
AoxI GGCC 5 cut(s) 3, 233, 266, 886, 896
ApaI GGGCCC 1 cut(s) 237
ApeKI GCWGC 3 cut(s) 105, 134, 338
ApoI RAATTY 3 cut(s) 150, 190, 802
AspLEI GCGC 1 cut(s) 139
AspS9I GGNCC 3 cut(s) 233, 234, 886
AsuC2I CCSGG 2 cut(s) 223, 237
AsuHPI GGTGA 3 cut(s) 704, 785, 938
BaeGI GKGCMC 1 cut(s) 237
BanII GRGCYC 2 cut(s) 80, 237
BbsI GAAGAC 3 cut(s) 504, 693, 738
Bbv12I GWGCWC 1 cut(s) 80
BbvI GCAGC 3 cut(s) 92, 121, 325
BccI CCATC 3 cut(s) 574, 605, 956
BceAI ACGGC 2 cut(s) 83, 124
BclI TGATCA 3 cut(s) 402, 570, 943
BcnI CCSGG 2 cut(s) 223, 237
BfaI CTAG 2 cut(s) 315, 351
BfmI CTRYAG 3 cut(s) 339, 843, 987
BisI GCNGC 5 cut(s) 24, 106, 109, 135, 339
BlsI GCNGC 5 cut(s) 25, 107, 110, 136, 340
Bme1390I CCNGG 2 cut(s) 223, 237
BmgBI CACGTC 1 cut(s) 72
BmgT120I GGNCC 3 cut(s) 233, 234, 886
BmiI GGNNCC 4 cut(s) 97, 235, 246, 864
BmrFI CCNGG 2 cut(s) 223, 237
BmrI ACTGGG 1 cut(s) 772
BmsI GCATC 2 cut(s) 224, 943
BmuI ACTGGG 1 cut(s) 772
BpiI GAAGAC 3 cut(s) 504, 693, 738
BpmI CTGGAG 1 cut(s) 849
BpuMI CCSGG 2 cut(s) 223, 237
BsaJI CCNNGG 2 cut(s) 140, 155
BsaWI WCCGGW 2 cut(s) 92, 436
BsaXI ACNNNNNCTCC 2 cut(s) 23, 53
Bsc4I CCNNNNNNNGG 2 cut(s) 286, 364
Bse1I ACTGG 3 cut(s) 767, 866, 949
BseAI TCCGGA 1 cut(s) 436
BseDI CCNNGG 2 cut(s) 140, 155
BseGI GGATG 2 cut(s) 616, 754
BseLI CCNNNNNNNGG 2 cut(s) 286, 364
BseMII CTCAG 1 cut(s) 93
BseNI ACTGG 3 cut(s) 767, 866, 949
BseRI GAGGAG 2 cut(s) 31, 683
BseSI GKGCMC 1 cut(s) 237
BseXI GCAGC 3 cut(s) 92, 121, 325
BsgI GTGCAG 1 cut(s) 885
BshFI GGCC 5 cut(s) 5, 235, 268, 888, 898
BsiHKAI GWGCWC 1 cut(s) 80
BsiSI CCGG 6 cut(s) 93, 123, 223, 237, 269, 437
BsiWI CGTACG 1 cut(s) 100
BslI CCNNNNNNNGG 2 cut(s) 286, 364
BsnI GGCC 5 cut(s) 5, 235, 268, 888, 898
Bsp120I GGGCCC 1 cut(s) 233
Bsp1286I GDGCHC 2 cut(s) 80, 237
Bsp13I TCCGGA 1 cut(s) 436
Bsp143I GATC 6 cut(s) 402, 570, 742, 838, 943, 1007
BspACI CCGC 4 cut(s) 21, 24, 109, 126
BspANI GGCC 5 cut(s) 5, 235, 268, 888, 898
BspCNI CTCAG 1 cut(s) 92
BspEI TCCGGA 1 cut(s) 436
BspLI GGNNCC 4 cut(s) 97, 235, 246, 864
BspMAI CTGCAG 1 cut(s) 343
BspPI GGATC 2 cut(s) 833, 1002
BsrI ACTGG 3 cut(s) 767, 866, 949
BssECI CCNNGG 2 cut(s) 140, 155
BssMI GATC 6 cut(s) 402, 570, 742, 838, 943, 1007
Bst4CI ACNGT 3 cut(s) 159, 692, 988
BstAPI GCANNNNNTGC 2 cut(s) 347, 781
BstC8I GCNNGC 2 cut(s) 861, 900
BstDEI CTNAG 2 cut(s) 79, 818
BstDSI CCRYGG 2 cut(s) 140, 155
BstENI CCTNNNNNAGG 1 cut(s) 362
BstF5I GGATG 2 cut(s) 616, 754
BstHHI GCGC 1 cut(s) 139
BstKTI GATC 6 cut(s) 405, 573, 745, 841, 946, 1010
BstMBI GATC 6 cut(s) 402, 570, 742, 838, 943, 1007
BstMWI GCNNNNNNNGC 6 cut(s) 117, 232, 253, 347, 781, 860
BstSCI CCNGG 2 cut(s) 221, 235
BstSFI CTRYAG 3 cut(s) 339, 843, 987
BstSLI GKGCMC 1 cut(s) 237
BstV1I GCAGC 3 cut(s) 92, 121, 325
BstV2I GAAGAC 3 cut(s) 504, 693, 738
BsuRI GGCC 5 cut(s) 5, 235, 268, 888, 898
BtgI CCRYGG 2 cut(s) 140, 155
BtrI CACGTC 1 cut(s) 72
BtsCI GGATG 2 cut(s) 616, 754
Cac8I GCNNGC 2 cut(s) 861, 900
CaiI CAGNNNCTG 1 cut(s) 347
CfoI GCGC 1 cut(s) 139
Cfr13I GGNCC 3 cut(s) 233, 234, 886
Csp6I GTAC 2 cut(s) 8, 101
CviAII CATG 2 cut(s) 52, 464
CviQI GTAC 2 cut(s) 8, 101
DdeI CTNAG 2 cut(s) 79, 818
DpnI GATC 6 cut(s) 404, 572, 744, 840, 945, 1009
DpnII GATC 6 cut(s) 402, 570, 742, 838, 943, 1007
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 1 cut(s) 10
Ecl136II GAGCTC 1 cut(s) 78
Eco147I AGGCCT 1 cut(s) 898
Eco24I GRGCYC 2 cut(s) 80, 237
Eco53kI GAGCTC 1 cut(s) 78
Eco57I CTGAAG 1 cut(s) 504
EcoICRI GAGCTC 1 cut(s) 78
EcoNI CCTNNNNNAGG 1 cut(s) 362
EcoO109I RGGNCCY 2 cut(s) 233, 886
EcoRI GAATTC 1 cut(s) 150
EcoT38I GRGCYC 2 cut(s) 80, 237
FaeI CATG 2 cut(s) 55, 467
FatI CATG 2 cut(s) 51, 463
FauI CCCGC 1 cut(s) 119
FbaI TGATCA 3 cut(s) 402, 570, 943
Fnu4HI GCNGC 5 cut(s) 24, 106, 109, 135, 339
FokI GGATG 2 cut(s) 623, 761
FriOI GRGCYC 2 cut(s) 80, 237
Fsp4HI GCNGC 5 cut(s) 24, 106, 109, 135, 339
FspBI CTAG 2 cut(s) 315, 351
GlaI GCGC 1 cut(s) 138
GluI GCNGC 5 cut(s) 24, 106, 109, 135, 339
GsuI CTGGAG 1 cut(s) 849
HaeIII GGCC 5 cut(s) 5, 235, 268, 888, 898
HapII CCGG 6 cut(s) 93, 123, 223, 237, 269, 437
HhaI GCGC 1 cut(s) 139
Hin1II CATG 2 cut(s) 55, 467
Hin6I GCGC 1 cut(s) 137
HinP1I GCGC 1 cut(s) 137
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HinfI GANTC 6 cut(s) 145, 208, 299, 344, 701, 752
HpaI GTTAAC 1 cut(s) 370
HpaII CCGG 6 cut(s) 93, 123, 223, 237, 269, 437
HphI GGTGA 3 cut(s) 704, 785, 938
Hpy166II GTNNAC 4 cut(s) 144, 303, 370, 418
Hpy188I TCNGA 8 cut(s) 69, 82, 244, 523, 706, 757, 835, 943
Hpy188III TCNNGA 4 cut(s) 419, 437, 722, 746
Hpy8I GTNNAC 4 cut(s) 144, 303, 370, 418
Hpy99I CGWCG 1 cut(s) 73
HpyAV CCTTC 2 cut(s) 481, 620
HpyCH4III ACNGT 3 cut(s) 159, 692, 988
HpyCH4IV ACGT 1 cut(s) 71
HpyCH4V TGCA 5 cut(s) 179, 341, 538, 775, 902
HpyF10VI GCNNNNNNNGC 6 cut(s) 117, 232, 253, 347, 781, 860
HpyF3I CTNAG 2 cut(s) 79, 818
HpySE526I ACGT 1 cut(s) 71
Hsp92II CATG 2 cut(s) 55, 467
HspAI GCGC 1 cut(s) 137
Kpn2I TCCGGA 1 cut(s) 436
Ksp22I TGATCA 3 cut(s) 402, 570, 943
KspAI GTTAAC 1 cut(s) 370
Kzo9I GATC 6 cut(s) 402, 570, 742, 838, 943, 1007
LmnI GCTCC 6 cut(s) 44, 75, 95, 125, 244, 868
Lsp1109I GCAGC 3 cut(s) 92, 121, 325
LweI GCATC 2 cut(s) 224, 943
MaeI CTAG 2 cut(s) 315, 351
MaeII ACGT 1 cut(s) 71
MaeIII GTNAC 4 cut(s) 451, 585, 692, 785
MalI GATC 6 cut(s) 404, 572, 744, 840, 945, 1009
MboI GATC 6 cut(s) 402, 570, 742, 838, 943, 1007
MhlI GDGCHC 2 cut(s) 80, 237
MluCI AATT 6 cut(s) 11, 150, 190, 594, 770, 802
MlyI GAGTC 2 cut(s) 139, 308
MmeI TCCRAC 1 cut(s) 92
MroI TCCGGA 1 cut(s) 436
MseI TTAA 1 cut(s) 369
MslI CAYNNNNRTG 1 cut(s) 50
MspA1I CMGCKG 1 cut(s) 338
MspI CCGG 6 cut(s) 93, 123, 223, 237, 269, 437
MspR9I CCNGG 2 cut(s) 223, 237
MwoI GCNNNNNNNGC 6 cut(s) 117, 232, 253, 347, 781, 860
NciI CCSGG 2 cut(s) 223, 237
NdeII GATC 6 cut(s) 402, 570, 742, 838, 943, 1007
NlaIII CATG 2 cut(s) 55, 467
NlaIV GGNNCC 4 cut(s) 97, 235, 246, 864
NmeAIII GCCGAG 1 cut(s) 322
NmuCI GTSAC 1 cut(s) 692
PceI AGGCCT 1 cut(s) 898
PcsI WCGNNNNNNNCGW 2 cut(s) 417, 672
PfeI GAWTC 4 cut(s) 208, 344, 701, 752
Pfl23II CGTACG 1 cut(s) 100
PkrI GCNGC 5 cut(s) 25, 107, 110, 136, 340
PleI GAGTC 2 cut(s) 139, 307
PpsI GAGTC 2 cut(s) 139, 307
PsiI TTATAA 1 cut(s) 893
Psp124BI GAGCTC 1 cut(s) 80
PspLI CGTACG 1 cut(s) 100
PspN4I GGNNCC 4 cut(s) 97, 235, 246, 864
PspOMI GGGCCC 1 cut(s) 233
PspPI GGNCC 3 cut(s) 233, 234, 886
PstI CTGCAG 1 cut(s) 343
PstNI CAGNNNCTG 1 cut(s) 347
PvuII CAGCTG 1 cut(s) 338
RsaI GTAC 2 cut(s) 9, 102
RsaNI GTAC 2 cut(s) 8, 101
RseI CAYNNNNRTG 1 cut(s) 50
SacI GAGCTC 1 cut(s) 80
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 5 cut(s) 24, 106, 109, 135, 339
Sau3AI GATC 6 cut(s) 402, 570, 742, 838, 943, 1007
Sau96I GGNCC 3 cut(s) 233, 234, 886
SchI GAGTC 2 cut(s) 139, 308
ScrFI CCNGG 2 cut(s) 223, 237
SduI GDGCHC 2 cut(s) 80, 237
SfaNI GCATC 2 cut(s) 224, 943
SfcI CTRYAG 3 cut(s) 339, 843, 987
SmiMI CAYNNNNRTG 1 cut(s) 50
Sse9I AATT 6 cut(s) 11, 150, 190, 594, 770, 802
SseBI AGGCCT 1 cut(s) 898
SsiI CCGC 4 cut(s) 21, 24, 109, 126
SspI AATATT 1 cut(s) 427
SspMI CTAG 2 cut(s) 315, 351
SstI GAGCTC 1 cut(s) 80
StuI AGGCCT 1 cut(s) 898
StyD4I CCNGG 2 cut(s) 221, 235
TaaI ACNGT 3 cut(s) 159, 692, 988
TaiI ACGT 1 cut(s) 74
TaqI TCGA 2 cut(s) 148, 395
TasI AATT 6 cut(s) 11, 150, 190, 594, 770, 802
TauI GCSGC 2 cut(s) 26, 111
TfiI GAWTC 4 cut(s) 208, 344, 701, 752
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TseFI GTSAC 1 cut(s) 692
TseI GCWGC 3 cut(s) 105, 134, 338
Tsp45I GTSAC 1 cut(s) 692
TspDTI ATGAA 6 cut(s) 474, 639, 696, 744, 815, 987
XagI CCTNNNNNAGG 1 cut(s) 362
XapI RAATTY 3 cut(s) 150, 190, 802
XspI CTAG 2 cut(s) 315, 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.