Rh1CG402600

At3g49140-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0006155
Physical Location & Seq
Reverse (-)
66820355 .. 66823941
3587 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG402600.1

Sequence Viewer

Length: 1377 bp
ATGATGATGATGATCGAGTCAGCCATGGCCGTACGATTCAACTCCGCCGCCGCCAATGTCTGCTCCTCCACCGCCGTCCCTTGTTTTCGTCCACGGTGGAGCTCGGAGGAGCTGACCGGAGCAGTCCACGTCACCTCTTGCCGGCTTGCGTACTCCGGCGGCTGGGGTAGGATTCGGAGATCAAAGCATTATGATTCGGTTGCGAAAAGGAGCAATTGTGTAAAGAATGGGATTCGAGCTGCCACGGAGCAGTTAGGGCCTGGTTCGGAGCCTGTGAAGCCGAATGGGAGGCCACAGTACCATCCGTTTGAGGATATTGCTGAAGCAACATTGGATAATGTTGGAGCTGCAAGACTTACATCTGCAGAATCTGCTAGGACCATTATTGAGGTTAATAGCAAAGCGACGCTCATGTTTTCGAGTCTGATTAATGATGAGGTTCATGAAAATATTATGTGTCCTGATTTGCCTTATGTAACTGATGAACATGGAAATATATACTTTCAAGTAAAGGATGGTGAAGACAATGCGTCTATGCAATCCATAACTTCAGAGAACAACTTTGTGGATTGGGTTTCCGTTCTTGACGACGAAGATGAAGAAGATGACGATGAAGATGACGAGACTCTTGGAGACTGGGCAAAGCTGGAGACTATGCGTTATTCTCACCCAATGTATTTTGCCAAGAAGCTGGCTGAGGTTGCTTCAGATGATCCTATAGATTGGGCAGAGCAGGCTCCAGCTAGTTTGGCAATTCAGGGGCTTCTAAGACCTGCATATATTGAAGAACATACGGTCATAAAAAAGCATATGTCGGATCATGAGCCCAATGACGATGAAAAACAAGTTGGGAGAACTGTAGAAGATGATTTAGAAGAGCCTGATAAGATCAATGGCCATGAATCAGGATCTTTCAAGGGCAGTCCAATCCGAGCGGAGGAATCAGACAATGAGAAGGGTGAAATACCAAAGAATGGGACTACTTTTTACAAACTGGAGATTGTGAAGATTCAGTTGTTTTCATCACATGGACATCAGAGTGTTGTTGAAGTAGAAGACTTCATAAAAGCTCAGCCTGATGCCATTGCACACTCAGCTGCTAAAATTATATCTCGTCTGAAAGCAGGTGGAGAAAAGACCACACAAGCTCTCAAGTCCCTTTGTTGGAGACTAAAGGGTATTCAAGTTGAGGAGGTAGTACTTACCGGTGTAGATTCTCTTGGATTTGACTTGAGGGTTTGCTCAGGAACACAAGTTCAGACGTTGCGGTTTGCACTAAATGCACAGGCCACTTCTGAGTATAGTGCTGAGAGACAACTTAATGATCTGCTATTCCCAAGGACACAGCACACGCCACAGAAAGTAAAGCAAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

458

Amino Acids

50.87

Weight (kDa)

4.94

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1115
Acc36I ACCTGC 2 cut(s) 781, 1115
AccB7I CCANNNNNTGG 1 cut(s) 974
AccBSI CCGCTC 1 cut(s) 935
AciI CCGC 7 cut(s) 45, 48, 51, 72, 159, 935, 1267
AclWI GGATC 3 cut(s) 707, 825, 916
AcoI YGGCCR 2 cut(s) 27, 895
AcuI CTGAAG 3 cut(s) 342, 534, 690
AfaI GTAC 4 cut(s) 33, 152, 299, 1200
AfiI CCNNNNNNNGG 5 cut(s) 141, 162, 937, 974, 1164
AgeI ACCGGT 1 cut(s) 1205
AgsI TTSAA 6 cut(s) 40, 506, 785, 916, 1049, 1184
AhdI GACNNNNNGTC 1 cut(s) 529
AjiI CACGTC 1 cut(s) 130
AjnI CCWGG 1 cut(s) 259
AjuI GAANNNNNNNTTGG 2 cut(s) 831, 863
AloI GAACNNNNNNTCC 2 cut(s) 1239, 1271
Alw21I GWGCWC 1 cut(s) 104
Alw26I GTCTC 5 cut(s) 617, 627, 644, 1162, 1306
AlwI GGATC 3 cut(s) 707, 825, 916
AlwNI CAGNNNCTG 1 cut(s) 371
AoxI GGCC 5 cut(s) 27, 257, 290, 895, 1287
ApeKI GCWGC 3 cut(s) 239, 347, 1097
AseI ATTAAT 1 cut(s) 429
AsiGI ACCGGT 1 cut(s) 1205
AspS9I GGNCC 2 cut(s) 257, 378
AsuHPI GGTGA 4 cut(s) 124, 530, 659, 971
AvaII GGWCC 1 cut(s) 378
BalI TGGCCA 1 cut(s) 897
BanII GRGCYC 2 cut(s) 104, 828
BbsI GAAGAC 2 cut(s) 528, 1062
Bbv12I GWGCWC 1 cut(s) 104
BbvCI CCTCAGC 1 cut(s) 696
BbvI GCAGC 3 cut(s) 226, 334, 1084
BccI CCATC 2 cut(s) 309, 509
BceAI ACGGC 2 cut(s) 14, 59
BciT130I CCWGG 1 cut(s) 261
BcoDI GTCTC 5 cut(s) 617, 627, 644, 1162, 1306
BfaI CTAG 2 cut(s) 375, 744
BfmI CTRYAG 3 cut(s) 363, 717, 858
BfuAI ACCTGC 2 cut(s) 781, 1115
BisI GCNGC 6 cut(s) 48, 51, 160, 240, 348, 1098
BlpI GCTNAGC 1 cut(s) 1071
BlsI GCNGC 6 cut(s) 49, 52, 161, 241, 349, 1099
BmcAI AGTACT 1 cut(s) 1200
Bme1390I CCNGG 1 cut(s) 261
Bme18I GGWCC 1 cut(s) 378
BmeRI GACNNNNNGTC 1 cut(s) 529
BmgBI CACGTC 1 cut(s) 130
BmgT120I GGNCC 2 cut(s) 257, 378
BmiI GGNNCC 2 cut(s) 270, 738
BmrFI CCNGG 1 cut(s) 261
BmrI ACTGGG 1 cut(s) 646
BmsI GCATC 1 cut(s) 1069
BmuI ACTGGG 1 cut(s) 646
BpiI GAAGAC 2 cut(s) 528, 1062
BpmI CTGGAG 3 cut(s) 668, 723, 1016
Bpu10I CCTNAGC 2 cut(s) 696, 1243
Bpu1102I GCTNAGC 1 cut(s) 1071
BpuEI CTTGAG 2 cut(s) 1136, 1252
BsaBI GATNNNNATC 1 cut(s) 11
BsaJI CCNNGG 4 cut(s) 24, 92, 243, 1337
BsaWI WCCGGW 2 cut(s) 116, 1205
BsaXI ACNNNNNCTCC 2 cut(s) 202, 232
Bsc4I CCNNNNNNNGG 5 cut(s) 141, 162, 937, 974, 1164
Bse118I RCCGGY 2 cut(s) 141, 1205
Bse1I ACTGG 2 cut(s) 641, 999
Bse3DI GCAATG 1 cut(s) 1083
Bse8I GATNNNNATC 1 cut(s) 11
BseBI CCWGG 1 cut(s) 261
BseDI CCNNGG 4 cut(s) 24, 92, 243, 1337
BseGI GGATG 2 cut(s) 301, 520
BseJI GATNNNNATC 1 cut(s) 11
BseLI CCNNNNNNNGG 5 cut(s) 141, 162, 937, 974, 1164
BseMI GCAATG 1 cut(s) 1083
BseMII CTCAG 6 cut(s) 687, 1085, 1107, 1257, 1287, 1299
BseNI ACTGG 2 cut(s) 641, 999
BseRI GAGGAG 3 cut(s) 55, 122, 1205
BseXI GCAGC 3 cut(s) 226, 334, 1084
BseYI CCCAGC 1 cut(s) 162
BshFI GGCC 5 cut(s) 29, 259, 292, 897, 1289
BshTI ACCGGT 1 cut(s) 1205
BsiHKAI GWGCWC 1 cut(s) 104
BsiSI CCGG 4 cut(s) 117, 142, 156, 1206
BsiWI CGTACG 1 cut(s) 31
BslFI GGGAC 3 cut(s) 62, 991, 1141
BslI CCNNNNNNNGG 5 cut(s) 141, 162, 937, 974, 1164
BsmAI GTCTC 5 cut(s) 617, 627, 644, 1162, 1306
BsmFI GGGAC 3 cut(s) 62, 991, 1141
BsnI GGCC 5 cut(s) 29, 259, 292, 897, 1289
Bsp1286I GDGCHC 2 cut(s) 104, 828
Bsp143I GATC 7 cut(s) 12, 179, 712, 817, 888, 908, 1324
Bsp1720I GCTNAGC 1 cut(s) 1071
Bsp19I CCATGG 1 cut(s) 24
BspACI CCGC 7 cut(s) 45, 48, 51, 72, 159, 935, 1267
BspANI GGCC 5 cut(s) 29, 259, 292, 897, 1289
BspCNI CTCAG 6 cut(s) 688, 1084, 1106, 1256, 1288, 1300
BspHI TCATGA 2 cut(s) 442, 820
BspLI GGNNCC 2 cut(s) 270, 738
BspMAI CTGCAG 1 cut(s) 367
BspMI ACCTGC 2 cut(s) 781, 1115
BspPI GGATC 3 cut(s) 707, 825, 916
BspQI GCTCTTC 1 cut(s) 870
BsrBI CCGCTC 1 cut(s) 935
BsrDI GCAATG 1 cut(s) 1083
BsrFI RCCGGY 2 cut(s) 141, 1205
BsrI ACTGG 2 cut(s) 641, 999
BssAI RCCGGY 2 cut(s) 141, 1205
BssECI CCNNGG 4 cut(s) 24, 92, 243, 1337
BssMI GATC 7 cut(s) 12, 179, 712, 817, 888, 908, 1324
BssT1I CCWWGG 2 cut(s) 24, 1337
Bst2UI CCWGG 1 cut(s) 261
Bst4CI ACNGT 4 cut(s) 96, 297, 796, 859
Bst6I CTCTTC 1 cut(s) 870
BstAPI GCANNNNNTGC 2 cut(s) 371, 1280
BstC8I GCNNGC 4 cut(s) 143, 147, 693, 735
BstDEI CTNAG 7 cut(s) 696, 767, 1071, 1093, 1243, 1296, 1308
BstDSI CCRYGG 3 cut(s) 24, 92, 243
BstF5I GGATG 2 cut(s) 301, 520
BstKTI GATC 7 cut(s) 15, 182, 715, 820, 891, 911, 1327
BstMAI GTCTC 5 cut(s) 617, 627, 644, 1162, 1306
BstMBI GATC 7 cut(s) 12, 179, 712, 817, 888, 908, 1324
BstMWI GCNNNNNNNGC 8 cut(s) 256, 277, 371, 701, 734, 749, 1094, 1280
BstNI CCWGG 1 cut(s) 261
BstSCI CCNGG 1 cut(s) 259
BstSFI CTRYAG 3 cut(s) 363, 717, 858
BstV1I GCAGC 3 cut(s) 226, 334, 1084
BstV2I GAAGAC 2 cut(s) 528, 1062
BstX2I RGATCY 1 cut(s) 908
BstXI CCANNNNNNTGG 1 cut(s) 691
BstYI RGATCY 1 cut(s) 908
BsuRI GGCC 5 cut(s) 29, 259, 292, 897, 1289
BtgI CCRYGG 3 cut(s) 24, 92, 243
BtrI CACGTC 1 cut(s) 130
BtsCI GGATG 2 cut(s) 301, 520
BveI ACCTGC 2 cut(s) 781, 1115
Cac8I GCNNGC 4 cut(s) 143, 147, 693, 735
CaiI CAGNNNCTG 1 cut(s) 371
CciI TCATGA 2 cut(s) 442, 820
Cfr10I RCCGGY 2 cut(s) 141, 1205
Cfr13I GGNCC 2 cut(s) 257, 378
CseI GACGC 2 cut(s) 415, 519
Csp6I GTAC 4 cut(s) 32, 151, 298, 1199
CspAI ACCGGT 1 cut(s) 1205
CviAII CATG 7 cut(s) 25, 412, 443, 488, 821, 899, 1028
CviQI GTAC 4 cut(s) 32, 151, 298, 1199
DdeI CTNAG 7 cut(s) 696, 767, 1071, 1093, 1243, 1296, 1308
DpnI GATC 7 cut(s) 14, 181, 714, 819, 890, 910, 1326
DpnII GATC 7 cut(s) 12, 179, 712, 817, 888, 908, 1324
DriI GACNNNNNGTC 1 cut(s) 529
EaeI YGGCCR 2 cut(s) 27, 895
Eam1104I CTCTTC 1 cut(s) 870
Eam1105I GACNNNNNGTC 1 cut(s) 529
EarI CTCTTC 1 cut(s) 870
EciI GGCGGA 1 cut(s) 34
Ecl136II GAGCTC 1 cut(s) 102
Eco130I CCWWGG 2 cut(s) 24, 1337
Eco24I GRGCYC 2 cut(s) 104, 828
Eco47I GGWCC 1 cut(s) 378
Eco53kI GAGCTC 1 cut(s) 102
Eco57I CTGAAG 3 cut(s) 342, 534, 690
EcoICRI GAGCTC 1 cut(s) 102
EcoO109I RGGNCCY 1 cut(s) 257
EcoRII CCWGG 1 cut(s) 259
EcoT14I CCWWGG 2 cut(s) 24, 1337
EcoT38I GRGCYC 2 cut(s) 104, 828
ErhI CCWWGG 2 cut(s) 24, 1337
FaeI CATG 7 cut(s) 28, 415, 446, 491, 824, 902, 1031
FaqI GGGAC 3 cut(s) 62, 991, 1141
FatI CATG 7 cut(s) 24, 411, 442, 487, 820, 898, 1027
FauNDI CATATG 1 cut(s) 810
Fnu4HI GCNGC 6 cut(s) 48, 51, 160, 240, 348, 1098
FokI GGATG 2 cut(s) 288, 527
FriOI GRGCYC 2 cut(s) 104, 828
Fsp4HI GCNGC 6 cut(s) 48, 51, 160, 240, 348, 1098
FspBI CTAG 2 cut(s) 375, 744
GluI GCNGC 6 cut(s) 48, 51, 160, 240, 348, 1098
GsaI CCCAGC 1 cut(s) 166
GsuI CTGGAG 3 cut(s) 668, 723, 1016
HaeIII GGCC 5 cut(s) 29, 259, 292, 897, 1289
HapII CCGG 4 cut(s) 117, 142, 156, 1206
HgaI GACGC 2 cut(s) 415, 519
Hin1II CATG 7 cut(s) 28, 415, 446, 491, 824, 902, 1031
HpaII CCGG 4 cut(s) 117, 142, 156, 1206
HphI GGTGA 4 cut(s) 124, 530, 659, 971
Hpy166II GTNNAC 2 cut(s) 92, 127
Hpy188III TCNNGA 6 cut(s) 443, 461, 584, 821, 906, 1245
Hpy8I GTNNAC 2 cut(s) 92, 127
Hpy99I CGWCG 2 cut(s) 409, 593
HpyAV CCTTC 1 cut(s) 949
HpyCH4III ACNGT 4 cut(s) 96, 297, 796, 859
HpyCH4IV ACGT 2 cut(s) 129, 1262
HpyCH4V TGCA 7 cut(s) 350, 365, 538, 776, 1088, 1274, 1283
HpyF10VI GCNNNNNNNGC 8 cut(s) 256, 277, 371, 701, 734, 749, 1094, 1280
HpyF3I CTNAG 7 cut(s) 696, 767, 1071, 1093, 1243, 1296, 1308
HpySE526I ACGT 2 cut(s) 129, 1262
Hsp92II CATG 7 cut(s) 28, 415, 446, 491, 824, 902, 1031
KroI GCCGGC 1 cut(s) 141
KroNI GCCGGC 1 cut(s) 143
Kzo9I GATC 7 cut(s) 12, 179, 712, 817, 888, 908, 1324
LguI GCTCTTC 1 cut(s) 870
LmnI GCTCC 9 cut(s) 68, 99, 109, 119, 210, 247, 268, 344, 742
Lsp1109I GCAGC 3 cut(s) 226, 334, 1084
LweI GCATC 1 cut(s) 1069
MaeI CTAG 2 cut(s) 375, 744
MaeII ACGT 2 cut(s) 129, 1262
MaeIII GTNAC 2 cut(s) 130, 475
MalI GATC 7 cut(s) 14, 181, 714, 819, 890, 910, 1326
MbiI CCGCTC 1 cut(s) 935
MboI GATC 7 cut(s) 12, 179, 712, 817, 888, 908, 1324
MfeI CAATTG 1 cut(s) 214
MflI RGATCY 1 cut(s) 908
MhlI GDGCHC 2 cut(s) 104, 828
MlsI TGGCCA 1 cut(s) 897
MluCI AATT 3 cut(s) 214, 753, 1104
MluNI TGGCCA 1 cut(s) 897
MlyI GAGTC 3 cut(s) 26, 430, 619
MmeI TCCRAC 3 cut(s) 322, 795, 1145
Mox20I TGGCCA 1 cut(s) 897
MroNI GCCGGC 1 cut(s) 141
MscI TGGCCA 1 cut(s) 897
MseI TTAA 4 cut(s) 393, 429, 1320, 1375
MslI CAYNNNNRTG 1 cut(s) 1038
Msp20I TGGCCA 1 cut(s) 897
MspA1I CMGCKG 1 cut(s) 1097
MspI CCGG 4 cut(s) 117, 142, 156, 1206
MspR9I CCNGG 1 cut(s) 261
MunI CAATTG 1 cut(s) 214
MvaI CCWGG 1 cut(s) 261
MwoI GCNNNNNNNGC 8 cut(s) 256, 277, 371, 701, 734, 749, 1094, 1280
NaeI GCCGGC 1 cut(s) 143
NcoI CCATGG 1 cut(s) 24
NdeI CATATG 1 cut(s) 810
NdeII GATC 7 cut(s) 12, 179, 712, 817, 888, 908, 1324
NgoMIV GCCGGC 1 cut(s) 141
NlaIII CATG 7 cut(s) 28, 415, 446, 491, 824, 902, 1031
NlaIV GGNNCC 2 cut(s) 270, 738
NmuCI GTSAC 1 cut(s) 130
PagI TCATGA 2 cut(s) 442, 820
PaqCI CACCTGC 1 cut(s) 1115
PciSI GCTCTTC 1 cut(s) 870
PdiI GCCGGC 1 cut(s) 143
PfeI GAWTC 9 cut(s) 36, 172, 194, 232, 368, 902, 941, 1009, 1214
Pfl23II CGTACG 1 cut(s) 31
PflMI CCANNNNNTGG 1 cut(s) 974
PinAI ACCGGT 1 cut(s) 1205
PkrI GCNGC 6 cut(s) 49, 52, 161, 241, 349, 1099
PleI GAGTC 3 cut(s) 25, 429, 619
PpsI GAGTC 3 cut(s) 25, 429, 619
PshBI ATTAAT 1 cut(s) 429
Psp124BI GAGCTC 1 cut(s) 104
Psp6I CCWGG 1 cut(s) 259
PspFI CCCAGC 1 cut(s) 162
PspGI CCWGG 1 cut(s) 259
PspLI CGTACG 1 cut(s) 31
PspN4I GGNNCC 2 cut(s) 270, 738
PspPI GGNCC 2 cut(s) 257, 378
PstI CTGCAG 1 cut(s) 367
PstNI CAGNNNCTG 1 cut(s) 371
PsuI RGATCY 1 cut(s) 908
PvuII CAGCTG 1 cut(s) 1097
RsaI GTAC 4 cut(s) 33, 152, 299, 1200
RsaNI GTAC 4 cut(s) 32, 151, 298, 1199
RseI CAYNNNNRTG 1 cut(s) 1038
SacI GAGCTC 1 cut(s) 104
SapI GCTCTTC 1 cut(s) 870
SaqAI TTAA 4 cut(s) 393, 429, 1320, 1375
SatI GCNGC 6 cut(s) 48, 51, 160, 240, 348, 1098
Sau3AI GATC 7 cut(s) 12, 179, 712, 817, 888, 908, 1324
Sau96I GGNCC 2 cut(s) 257, 378
ScaI AGTACT 1 cut(s) 1200
SchI GAGTC 3 cut(s) 26, 430, 619
ScrFI CCNGG 1 cut(s) 261
SduI GDGCHC 2 cut(s) 104, 828
SfaNI GCATC 1 cut(s) 1069
SfcI CTRYAG 3 cut(s) 363, 717, 858
SinI GGWCC 1 cut(s) 378
SmiMI CAYNNNNRTG 1 cut(s) 1038
SmlI CTYRAG 2 cut(s) 1151, 1231
SmoI CTYRAG 2 cut(s) 1151, 1231
Sse9I AATT 3 cut(s) 214, 753, 1104
SsiI CCGC 7 cut(s) 45, 48, 51, 72, 159, 935, 1267
SspI AATATT 1 cut(s) 451
SspMI CTAG 2 cut(s) 375, 744
SstI GAGCTC 1 cut(s) 104
StyD4I CCNGG 1 cut(s) 259
StyI CCWWGG 2 cut(s) 24, 1337
TaaI ACNGT 4 cut(s) 96, 297, 796, 859
TaiI ACGT 2 cut(s) 132, 1265
TaqI TCGA 3 cut(s) 15, 235, 419
TasI AATT 3 cut(s) 214, 753, 1104
TatI WGTACW 1 cut(s) 1198
TauI GCSGC 3 cut(s) 50, 53, 162
TfiI GAWTC 9 cut(s) 36, 172, 194, 232, 368, 902, 941, 1009, 1214
Tru1I TTAA 4 cut(s) 393, 429, 1320, 1375
Tru9I TTAA 4 cut(s) 393, 429, 1320, 1375
TseFI GTSAC 1 cut(s) 130
TseI GCWGC 3 cut(s) 239, 347, 1097
Tsp45I GTSAC 1 cut(s) 130
TspDTI ATGAA 9 cut(s) 431, 459, 498, 612, 627, 852, 915, 1011, 1051
TspGWI ACGGA 3 cut(s) 260, 294, 568
Van91I CCANNNNNTGG 1 cut(s) 974
VpaK11BI GGWCC 1 cut(s) 378
VspI ATTAAT 1 cut(s) 429
XspI CTAG 2 cut(s) 375, 744
ZrmI AGTACT 1 cut(s) 1200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.