AT3G49260

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007423
Physical Location & Seq
Forward (+)
18262290 .. 18266021
3732 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G49260.1

Sequence Viewer

Length: 1416 bp
ATGGGGAAGAAAGGGAGTGGTGGATGGTTCTCGACGGTGAAGAAGAAAGTCTTCAAATCCTCTCCTAAAGACTCCAAGAGAGAGAACAACATTGGTAGTAATAACGCCGACATATGGCAGCAGCAGCATGATACGCAAGAAGTCGTGTCGTTTGAACATTTTCCGGCGGAAAGTTCACCGGAGATCTCCCATGACGTCGAGAGCACAGCATCTACTCCAGCAACTAATGTTGGAGACAGAAAACACGCTATGGCTGTAGCTATAGCCACTGCAGCTGCTGCCGAAGCCGCGGTTGCAGCCGCACAAGCTGCCGCTAAAGTCGTTCGACTTGCCGGCTACAACCGCCAGACGGAGGAAGATAGCGCCGCCGTGCTAATCCAATCGCACTATAGGGGTTATTTGGCGAGACGAGCACTTCGTGCCTTAAAAGGACTAGTTAGGCTACAAGCATTGGTCCGTGGCAACCACGTACGAAAACAGGCACAGATGACTATGAAATGTATGCAAGCATTGGTTCGTGTCCAAGGCCGAGTCAGAGCAAGAAGACTCCAAGTGGCTCATGACCGGTTTAAGAAACAGTTTGAGGAAGAAGAGAAGCGGTCCGGGATGGAAAAACCGAACAAGGGGTTTGCGAATCTCAAAACGGAACGAGAGAAGCCGAAGAAATTACATGAAGTGAATCGAACCAGTCTGTACCAAACTCAAGGAAAGGAAAAAGAGAGGAGTGAAGGAATGATGAAAAGAGAGAGGGCTCTAGCTTATGCGTACACTTACCAGCGCCAAATGCAACACACAAATAGTGAAGAAGGGATTGGGCTTTCTTCTAATGGGCCTGATAGAAACCAATGGGCTTGGAATTGGCTTGACCATTGGATGTCTTCCCAGCCATACACTGGCCGTCAAACTGGTCCGGGTCCCGGTCCCGGACAGTACAATCCTCCTCCGTATCCGCCTTTTCCGACAGCTGCGGCAACCACCACATCTGATGATGTATCGGAGAAGACGGTGGAAATGGACGTGACCACTCCGACTAGCCTCAAGGGCAATATAATCGGGCTTATTGATCGAGAGTATATAGACTTAGGATCATATAGACAAGGCCATAAGCAACGGAAAAGCCCAACCCATATACCAAGCTATATGGCTCCAACCGCATCTGCTAAGGCTAAAGTTCGAGATCAAGGCCCAACCGTCAAACTACAAGGAACTTCGTTTATGCCGTATTGGAACTCCTCGACCAAAAACGGGTCGGTTAATGGATCAGGTTGTGATTCGTCAAGTTCAGGTGGAGCAATAACAACTGGTTATCCCGGTCCTAGGAGTCCGAACCCAAAATCGGATATCCGACGTAAACCGGTTAGTCCTAGTCAAAGTCCTACCGGGTTTGGAAAGAGAGGCTGGAGGCATGATCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

471

Amino Acids

52.09

Weight (kDa)

9.95

Isoelectric Point (pI)

50.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IQ PF00612 121 - 140 1.5e-06 IQ calmodulin-binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 198
AccB7I CCANNNNNTGG 1 cut(s) 893
AccII CGCG 1 cut(s) 290
AclWI GGATC 2 cut(s) 1093, 1267
AcoI YGGCCR 1 cut(s) 895
AcyI GRCGYC 1 cut(s) 195
AdeI CACNNNGTG 1 cut(s) 419
AfaI GTAC 4 cut(s) 471, 695, 767, 932
AgeI ACCGGT 2 cut(s) 564, 1354
AgsI TTSAA 2 cut(s) 55, 155
AhlI ACTAGT 1 cut(s) 433
AjiI CACGTC 1 cut(s) 1018
AjuI GAANNNNNNNTTGG 2 cut(s) 795, 827
AluBI AGCT 6 cut(s) 260, 275, 308, 758, 965, 1137
AluI AGCT 6 cut(s) 260, 275, 308, 758, 965, 1137
Alw21I GWGCWC 2 cut(s) 206, 415
Alw26I GTCTC 2 cut(s) 228, 400
AlwI GGATC 2 cut(s) 1093, 1267
AlwNI CAGNNNCTG 1 cut(s) 278
AoxI GGCC 5 cut(s) 526, 830, 895, 1099, 1183
ApeKI GCWGC 9 cut(s) 118, 121, 124, 272, 275, 278, 296, 308, 965
ArsI GACNNNNNNTTYG 2 cut(s) 399, 431
AsiGI ACCGGT 2 cut(s) 564, 1354
Asp700I GAANNNNTTC 2 cut(s) 50, 159
AspA2I CCTAGG 1 cut(s) 1316
AspLEI GCGC 2 cut(s) 365, 780
AspS9I GGNCC 8 cut(s) 454, 600, 830, 908, 914, 920, 1184, 1313
AsuC2I CCSGG 6 cut(s) 604, 912, 918, 924, 1311, 1381
AsuHPI GGTGA 2 cut(s) 49, 168
AvaII GGWCC 6 cut(s) 454, 600, 908, 914, 920, 1313
AvrII CCTAGG 1 cut(s) 1316
BanII GRGCYC 1 cut(s) 754
BbsI GAAGAC 4 cut(s) 43, 550, 870, 1007
Bbv12I GWGCWC 2 cut(s) 206, 415
BbvI GCAGC 9 cut(s) 130, 133, 136, 262, 265, 284, 295, 308, 952
BccI CCATC 2 cut(s) 18, 601
BceAI ACGGC 3 cut(s) 353, 882, 1204
BcgI CGANNNNNNTGC 2 cut(s) 1033, 1067
BciVI GTATCC 1 cut(s) 957
BclI TGATCA 1 cut(s) 1408
BcnI CCSGG 6 cut(s) 604, 912, 918, 924, 1311, 1381
BcoDI GTCTC 2 cut(s) 228, 400
BcuI ACTAGT 1 cut(s) 433
BfaI CTAG 5 cut(s) 434, 755, 1032, 1317, 1365
BfmI CTRYAG 4 cut(s) 255, 261, 270, 388
BfoI RGCGCY 2 cut(s) 366, 781
BfuI GTATCC 1 cut(s) 957
BglI GCCNNNNNGGC 1 cut(s) 1041
BglII AGATCT 1 cut(s) 183
BlnI CCTAGG 1 cut(s) 1316
Bme1390I CCNGG 6 cut(s) 604, 912, 918, 924, 1311, 1381
Bme18I GGWCC 6 cut(s) 454, 600, 908, 914, 920, 1313
BmgBI CACGTC 1 cut(s) 1018
BmgT120I GGNCC 8 cut(s) 454, 600, 830, 908, 914, 920, 1184, 1313
BmiI GGNNCC 4 cut(s) 915, 916, 922, 1146
BmrFI CCNGG 6 cut(s) 604, 912, 918, 924, 1311, 1381
BmsI GCATC 2 cut(s) 218, 1163
BpiI GAAGAC 4 cut(s) 43, 550, 870, 1007
BpmI CTGGAG 1 cut(s) 201
Bpu10I CCTNAGC 1 cut(s) 1161
BpuEI CTTGAG 2 cut(s) 687, 1022
BpuMI CCSGG 6 cut(s) 604, 912, 918, 924, 1311, 1381
BsaAI YACGTR 1 cut(s) 469
BsaHI GRCGYC 1 cut(s) 195
BsaJI CCNNGG 4 cut(s) 288, 457, 523, 1316
BsaWI WCCGGW 3 cut(s) 178, 564, 1354
Bse118I RCCGGY 3 cut(s) 332, 564, 1354
Bse1I ACTGG 4 cut(s) 687, 898, 910, 1306
BseDI CCNNGG 4 cut(s) 288, 457, 523, 1316
BseGI GGATG 3 cut(s) 29, 612, 879
BseNI ACTGG 4 cut(s) 687, 898, 910, 1306
BseRI GAGGAG 3 cut(s) 736, 930, 1222
BseXI GCAGC 9 cut(s) 130, 133, 136, 262, 265, 284, 295, 308, 952
BseYI CCCAGC 1 cut(s) 882
Bsh1236I CGCG 1 cut(s) 290
BshFI GGCC 5 cut(s) 528, 832, 897, 1101, 1185
BshTI ACCGGT 2 cut(s) 564, 1354
BsiHKAI GWGCWC 2 cut(s) 206, 415
BsiWI CGTACG 1 cut(s) 469
BslFI GGGAC 2 cut(s) 900, 906
BsmAI GTCTC 2 cut(s) 228, 400
BsmBI CGTCTC 1 cut(s) 400
BsmFI GGGAC 2 cut(s) 900, 906
BsnI GGCC 5 cut(s) 528, 832, 897, 1101, 1185
Bsp1286I GDGCHC 3 cut(s) 206, 415, 754
Bsp143I GATC 6 cut(s) 183, 1063, 1085, 1177, 1259, 1408
BspANI GGCC 5 cut(s) 528, 832, 897, 1101, 1185
BspFNI CGCG 1 cut(s) 290
BspHI TCATGA 1 cut(s) 559
BspLI GGNNCC 4 cut(s) 915, 916, 922, 1146
BspMAI CTGCAG 1 cut(s) 274
BspPI GGATC 2 cut(s) 1093, 1267
BsrFI RCCGGY 3 cut(s) 332, 564, 1354
BsrI ACTGG 4 cut(s) 687, 898, 910, 1306
BssAI RCCGGY 3 cut(s) 332, 564, 1354
BssECI CCNNGG 4 cut(s) 288, 457, 523, 1316
BssMI GATC 6 cut(s) 183, 1063, 1085, 1177, 1259, 1408
BssNI GRCGYC 1 cut(s) 195
BssT1I CCWWGG 2 cut(s) 523, 1316
Bst4CI ACNGT 5 cut(s) 37, 579, 930, 1006, 1192
Bst6I CTCTTC 1 cut(s) 585
BstACI GRCGYC 1 cut(s) 195
BstAPI GCANNNNNTGC 3 cut(s) 278, 308, 419
BstBAI YACGTR 1 cut(s) 469
BstC8I GCNNGC 2 cut(s) 334, 507
BstDEI CTNAG 2 cut(s) 1081, 1161
BstDSI CCRYGG 2 cut(s) 288, 457
BstF5I GGATG 3 cut(s) 29, 612, 879
BstFNI CGCG 1 cut(s) 290
BstH2I RGCGCY 2 cut(s) 366, 781
BstHHI GCGC 2 cut(s) 365, 780
BstKTI GATC 6 cut(s) 186, 1066, 1088, 1180, 1262, 1411
BstMAI GTCTC 2 cut(s) 228, 400
BstMBI GATC 6 cut(s) 183, 1063, 1085, 1177, 1259, 1408
BstSCI CCNGG 6 cut(s) 602, 910, 916, 922, 1309, 1379
BstSFI CTRYAG 4 cut(s) 255, 261, 270, 388
BstUI CGCG 1 cut(s) 290
BstV1I GCAGC 9 cut(s) 130, 133, 136, 262, 265, 284, 295, 308, 952
BstV2I GAAGAC 4 cut(s) 43, 550, 870, 1007
BstX2I RGATCY 1 cut(s) 183
BstYI RGATCY 1 cut(s) 183
BsuI GTATCC 1 cut(s) 957
BsuRI GGCC 5 cut(s) 528, 832, 897, 1101, 1185
BtgI CCRYGG 2 cut(s) 288, 457
BtrI CACGTC 1 cut(s) 1018
BtsCI GGATG 3 cut(s) 29, 612, 879
BtsI GCAGTG 1 cut(s) 267
BtsIMutI CAGTG 2 cut(s) 267, 891
Cac8I GCNNGC 2 cut(s) 334, 507
CaiI CAGNNNCTG 1 cut(s) 278
CciI TCATGA 1 cut(s) 559
CfoI GCGC 2 cut(s) 365, 780
Cfr10I RCCGGY 3 cut(s) 332, 564, 1354
Cfr13I GGNCC 8 cut(s) 454, 600, 830, 908, 914, 920, 1184, 1313
Cfr42I CCGCGG 1 cut(s) 291
CpoI CGGWCCG 1 cut(s) 600
Csp6I GTAC 4 cut(s) 470, 694, 766, 931
CspAI ACCGGT 2 cut(s) 564, 1354
CspI CGGWCCG 1 cut(s) 600
CviAII CATG 5 cut(s) 128, 191, 560, 671, 1406
CviQI GTAC 4 cut(s) 470, 694, 766, 931
DdeI CTNAG 2 cut(s) 1081, 1161
DpnI GATC 6 cut(s) 185, 1065, 1087, 1179, 1261, 1410
DpnII GATC 6 cut(s) 183, 1063, 1085, 1177, 1259, 1408
DraIII CACNNNGTG 1 cut(s) 419
EaeI YGGCCR 1 cut(s) 895
Eam1104I CTCTTC 1 cut(s) 585
EarI CTCTTC 1 cut(s) 585
EciI GGCGGA 2 cut(s) 182, 939
Eco130I CCWWGG 2 cut(s) 523, 1316
Eco24I GRGCYC 1 cut(s) 754
Eco32I GATATC 1 cut(s) 1342
Eco47I GGWCC 6 cut(s) 454, 600, 908, 914, 920, 1313
EcoO109I RGGNCCY 1 cut(s) 914
EcoRV GATATC 1 cut(s) 1342
EcoT14I CCWWGG 2 cut(s) 523, 1316
EcoT38I GRGCYC 1 cut(s) 754
ErhI CCWWGG 2 cut(s) 523, 1316
Esp3I CGTCTC 1 cut(s) 400
FaeI CATG 5 cut(s) 131, 194, 563, 674, 1409
FalI AAGNNNNNCTT 2 cut(s) 35, 67
FaqI GGGAC 2 cut(s) 900, 906
FatI CATG 5 cut(s) 127, 190, 559, 670, 1405
FauNDI CATATG 1 cut(s) 113
FbaI TGATCA 1 cut(s) 1408
FokI GGATG 3 cut(s) 36, 619, 886
FriOI GRGCYC 1 cut(s) 754
FspBI CTAG 5 cut(s) 434, 755, 1032, 1317, 1365
GlaI GCGC 2 cut(s) 364, 779
GsaI CCCAGC 1 cut(s) 886
GsuI CTGGAG 1 cut(s) 201
HaeII RGCGCY 2 cut(s) 366, 781
HaeIII GGCC 5 cut(s) 528, 832, 897, 1101, 1185
HhaI GCGC 2 cut(s) 365, 780
Hin1I GRCGYC 1 cut(s) 195
Hin1II CATG 5 cut(s) 131, 194, 563, 674, 1409
Hin6I GCGC 2 cut(s) 363, 778
HinP1I GCGC 2 cut(s) 363, 778
HinfI GANTC 7 cut(s) 71, 531, 546, 634, 679, 1271, 1321
HphI GGTGA 2 cut(s) 49, 168
Hpy166II GTNNAC 3 cut(s) 176, 768, 1352
Hpy188I TCNGA 8 cut(s) 536, 960, 985, 997, 1029, 1326, 1339, 1346
Hpy188III TCNNGA 5 cut(s) 31, 199, 560, 1067, 1175
Hpy8I GTNNAC 3 cut(s) 176, 768, 1352
Hpy99I CGWCG 3 cut(s) 37, 200, 1350
HpyAV CCTTC 2 cut(s) 722, 800
HpyCH4III ACNGT 5 cut(s) 37, 579, 930, 1006, 1192
HpyCH4IV ACGT 4 cut(s) 195, 468, 1017, 1348
HpyCH4V TGCA 4 cut(s) 272, 296, 505, 787
HpyF3I CTNAG 2 cut(s) 1081, 1161
HpySE526I ACGT 4 cut(s) 195, 468, 1017, 1348
Hsp92I GRCGYC 1 cut(s) 195
Hsp92II CATG 5 cut(s) 131, 194, 563, 674, 1409
HspAI GCGC 2 cut(s) 363, 778
KflI GGGWCCC 1 cut(s) 914
KroI GCCGGC 1 cut(s) 332
KroNI GCCGGC 1 cut(s) 334
Ksp22I TGATCA 1 cut(s) 1408
KspI CCGCGG 1 cut(s) 291
Kzo9I GATC 6 cut(s) 183, 1063, 1085, 1177, 1259, 1408
LmnI GCTCC 2 cut(s) 1150, 1289
Lsp1109I GCAGC 9 cut(s) 130, 133, 136, 262, 265, 284, 295, 308, 952
LweI GCATC 2 cut(s) 218, 1163
MaeI CTAG 5 cut(s) 434, 755, 1032, 1317, 1365
MaeII ACGT 4 cut(s) 195, 468, 1017, 1348
MaeIII GTNAC 1 cut(s) 1018
MalI GATC 6 cut(s) 185, 1065, 1087, 1179, 1261, 1410
MboI GATC 6 cut(s) 183, 1063, 1085, 1177, 1259, 1408
MflI RGATCY 1 cut(s) 183
MhlI GDGCHC 3 cut(s) 206, 415, 754
MluCI AATT 2 cut(s) 665, 856
MlyI GAGTC 4 cut(s) 65, 540, 540, 1330
MmeI TCCRAC 5 cut(s) 211, 983, 1052, 1172, 1369
MroNI GCCGGC 1 cut(s) 332
MroXI GAANNNNTTC 2 cut(s) 50, 159
MseI TTAA 3 cut(s) 425, 570, 1254
MspA1I CMGCKG 3 cut(s) 275, 290, 965
MspR9I CCNGG 6 cut(s) 604, 912, 918, 924, 1311, 1381
MvnI CGCG 1 cut(s) 290
NaeI GCCGGC 1 cut(s) 334
NciI CCSGG 6 cut(s) 604, 912, 918, 924, 1311, 1381
NdeI CATATG 1 cut(s) 113
NdeII GATC 6 cut(s) 183, 1063, 1085, 1177, 1259, 1408
NgoMIV GCCGGC 1 cut(s) 332
NlaIII CATG 5 cut(s) 131, 194, 563, 674, 1409
NlaIV GGNNCC 4 cut(s) 915, 916, 922, 1146
NmeAIII GCCGAG 1 cut(s) 554
NmuCI GTSAC 1 cut(s) 1018
PagI TCATGA 1 cut(s) 559
PcsI WCGNNNNNNNCGW 2 cut(s) 415, 1217
PdiI GCCGGC 1 cut(s) 334
PdmI GAANNNNTTC 2 cut(s) 50, 159
PfeI GAWTC 3 cut(s) 634, 679, 1271
Pfl23II CGTACG 1 cut(s) 469
PflMI CCANNNNNTGG 1 cut(s) 893
PfoI TCCNGGA 2 cut(s) 602, 922
PinAI ACCGGT 2 cut(s) 564, 1354
PleI GAGTC 4 cut(s) 65, 539, 540, 1329
PpsI GAGTC 4 cut(s) 65, 539, 540, 1329
Ppu21I YACGTR 1 cut(s) 469
PpuMI RGGWCCY 1 cut(s) 914
Psp5II RGGWCCY 1 cut(s) 914
PspFI CCCAGC 1 cut(s) 882
PspLI CGTACG 1 cut(s) 469
PspN4I GGNNCC 4 cut(s) 915, 916, 922, 1146
PspPI GGNCC 8 cut(s) 454, 600, 830, 908, 914, 920, 1184, 1313
PspPPI RGGWCCY 1 cut(s) 914
PstI CTGCAG 1 cut(s) 274
PstNI CAGNNNCTG 1 cut(s) 278
PsuI RGATCY 1 cut(s) 183
PvuII CAGCTG 2 cut(s) 275, 965
RsaI GTAC 4 cut(s) 471, 695, 767, 932
RsaNI GTAC 4 cut(s) 470, 694, 766, 931
Rsr2I CGGWCCG 1 cut(s) 600
RsrII CGGWCCG 1 cut(s) 600
SacII CCGCGG 1 cut(s) 291
SaqAI TTAA 3 cut(s) 425, 570, 1254
Sau3AI GATC 6 cut(s) 183, 1063, 1085, 1177, 1259, 1408
Sau96I GGNCC 8 cut(s) 454, 600, 830, 908, 914, 920, 1184, 1313
SchI GAGTC 4 cut(s) 65, 540, 540, 1330
ScrFI CCNGG 6 cut(s) 604, 912, 918, 924, 1311, 1381
SduI GDGCHC 3 cut(s) 206, 415, 754
SfaNI GCATC 2 cut(s) 218, 1163
SfcI CTRYAG 4 cut(s) 255, 261, 270, 388
Sfr303I CCGCGG 1 cut(s) 291
SgrBI CCGCGG 1 cut(s) 291
SinI GGWCC 6 cut(s) 454, 600, 908, 914, 920, 1313
SmlI CTYRAG 2 cut(s) 702, 1037
SmoI CTYRAG 2 cut(s) 702, 1037
SpeI ACTAGT 1 cut(s) 433
Sse9I AATT 2 cut(s) 665, 856
SspMI CTAG 5 cut(s) 434, 755, 1032, 1317, 1365
StyD4I CCNGG 6 cut(s) 602, 910, 916, 922, 1309, 1379
StyI CCWWGG 2 cut(s) 523, 1316
TaaI ACNGT 5 cut(s) 37, 579, 930, 1006, 1192
TaiI ACGT 4 cut(s) 198, 471, 1020, 1351
TaqI TCGA 7 cut(s) 32, 198, 325, 682, 1066, 1174, 1235
TasI AATT 2 cut(s) 665, 856
TatI WGTACW 1 cut(s) 930
TauI GCSGC 5 cut(s) 290, 302, 314, 368, 971
TfiI GAWTC 3 cut(s) 634, 679, 1271
Tru1I TTAA 3 cut(s) 425, 570, 1254
Tru9I TTAA 3 cut(s) 425, 570, 1254
TscAI CASTG 2 cut(s) 274, 898
TseFI GTSAC 1 cut(s) 1018
TseI GCWGC 9 cut(s) 118, 121, 124, 272, 275, 278, 296, 308, 965
Tsp45I GTSAC 1 cut(s) 1018
TspDTI ATGAA 3 cut(s) 509, 687, 752
TspGWI ACGGA 5 cut(s) 365, 446, 659, 933, 1126
TspRI CASTG 2 cut(s) 274, 898
Van91I CCANNNNNTGG 1 cut(s) 893
VpaK11BI GGWCC 6 cut(s) 454, 600, 908, 914, 920, 1313
XcmI CCANNNNNNNNNTGG 1 cut(s) 890
XmaJI CCTAGG 1 cut(s) 1316
XmnI GAANNNNTTC 2 cut(s) 50, 159
XspI CTAG 5 cut(s) 434, 755, 1032, 1317, 1365
ZraI GACGTC 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.