AT3G52630

Replication protein A 14 kDa subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0013004
Physical Location & Seq
Forward (+)
19513937 .. 19514868
932 bp
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UTR
Exon/CDS
Intron
AT3G52630.1

Sequence Viewer

Length: 324 bp
ATGGATACTTCAAGTCCTTCAGCTTTTGTGAACGGAGCTTTGTTGAGGAGATTCATTGGCCAAAAAGTCAGAACTGTGATTCAAGTGACTGGTTCAGAGATTGGATCTGTGGTTGGCAAATCTACTGATGATCTTCAGATCGTTGTTAGAGGATCGTCTCCTCCTTCTCCTCTTACTACTTACCTTGAGGTTATTGGAATTGCAGAGAGTGATAACGCTATTCGAGCTGAAACTTGGACCAATTTTGGCAACACTTTTGATACACAGAACTACAACGAGCTATGTAAGCTAGCAAATGGGGAGTTCAAGCACTTGTTCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
Rep_fac-A_3

Protein Analysis

107

Amino Acids

11.65

Weight (kDa)

5.24

Isoelectric Point (pI)

24.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep_fac-A_3 PF08661 1 - 104 2.1e-27 Replication factor A protein 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013004)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52630 AT3G52630 AT4G18590
fragaria_vesca FvH4_6g18910
malus_domestica MD12G1025300.v1.1 MD14G1024000.v1.1
prunus_persica Prupe.7G108800_v2.0.a1
pyrus_communis pycom12g02480
rosa_chinensis RchiOBHm_Chr3g0473901
rosa_laevigata RLG00000023972
rosa_multiflora Rmu_sc0002414.1_g000040
rosa_roxburghii Rroxscaffold_6G00407670
rosa_rugosa Rorug03G0136800
rosa_samantha Rh3AG187000 Rh3BG215600 Rh3CG212300 Rh3DG211500
rosa_wichuraiana Rw3G017120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 112, 160
AcoI YGGCCR 1 cut(s) 58
AcuI CTGAAG 1 cut(s) 119
AgsI TTSAA 3 cut(s) 12, 83, 307
AluBI AGCT 5 cut(s) 23, 38, 227, 280, 289
AluI AGCT 5 cut(s) 23, 38, 227, 280, 289
Alw26I GTCTC 1 cut(s) 162
AlwI GGATC 2 cut(s) 112, 160
AoxI GGCC 1 cut(s) 58
AspS9I GGNCC 1 cut(s) 237
AsuNHI GCTAGC 1 cut(s) 289
AvaII GGWCC 1 cut(s) 237
BalI TGGCCA 1 cut(s) 60
BcoDI GTCTC 1 cut(s) 162
BfaI CTAG 1 cut(s) 290
Bme18I GGWCC 1 cut(s) 237
BmgT120I GGNCC 1 cut(s) 237
BmtI GCTAGC 1 cut(s) 293
BpuEI CTTGAG 1 cut(s) 206
Bse1I ACTGG 1 cut(s) 94
BseNI ACTGG 1 cut(s) 94
BseRI GAGGAG 3 cut(s) 61, 150, 159
BshFI GGCC 1 cut(s) 60
BsmAI GTCTC 1 cut(s) 162
BsmBI CGTCTC 1 cut(s) 162
BsnI GGCC 1 cut(s) 60
Bsp143I GATC 4 cut(s) 104, 130, 138, 152
BspANI GGCC 1 cut(s) 60
BspOI GCTAGC 1 cut(s) 293
BspPI GGATC 2 cut(s) 112, 160
BsrI ACTGG 1 cut(s) 94
BssMI GATC 4 cut(s) 104, 130, 138, 152
Bst4CI ACNGT 1 cut(s) 76
BstC8I GCNNGC 1 cut(s) 291
BstKTI GATC 4 cut(s) 107, 133, 141, 155
BstMAI GTCTC 1 cut(s) 162
BstMBI GATC 4 cut(s) 104, 130, 138, 152
BstMWI GCNNNNNNNGC 2 cut(s) 224, 286
BstX2I RGATCY 1 cut(s) 104
BstYI RGATCY 1 cut(s) 104
BsuRI GGCC 1 cut(s) 60
Cac8I GCNNGC 1 cut(s) 291
Cfr13I GGNCC 1 cut(s) 237
CviJI RGCY 6 cut(s) 23, 38, 60, 227, 280, 289
CviKI_1 RGCY 6 cut(s) 23, 38, 60, 227, 280, 289
DpnI GATC 4 cut(s) 106, 132, 140, 154
DpnII GATC 4 cut(s) 104, 130, 138, 152
EaeI YGGCCR 1 cut(s) 58
Eco47I GGWCC 1 cut(s) 237
Eco57I CTGAAG 1 cut(s) 119
Esp3I CGTCTC 1 cut(s) 162
FaiI YATR 1 cut(s) 283
FspBI CTAG 1 cut(s) 290
HaeIII GGCC 1 cut(s) 60
HinfI GANTC 2 cut(s) 51, 79
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 3 cut(s) 71, 97, 138
Hpy8I GTNNAC 1 cut(s) 31
HpyAV CCTTC 2 cut(s) 27, 174
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4V TGCA 1 cut(s) 203
HpyF10VI GCNNNNNNNGC 2 cut(s) 224, 286
Kzo9I GATC 4 cut(s) 104, 130, 138, 152
LmnI GCTCC 1 cut(s) 35
LpnPI CCDG 1 cut(s) 75
MaeI CTAG 1 cut(s) 290
MaeIII GTNAC 1 cut(s) 85
MalI GATC 4 cut(s) 106, 132, 140, 154
MboI GATC 4 cut(s) 104, 130, 138, 152
MboII GAAGA 1 cut(s) 125
MflI RGATCY 1 cut(s) 104
MlsI TGGCCA 1 cut(s) 60
MluCI AATT 2 cut(s) 198, 241
MluNI TGGCCA 1 cut(s) 60
MnlI CCTC 5 cut(s) 39, 143, 171, 180, 181
Mox20I TGGCCA 1 cut(s) 60
MscI TGGCCA 1 cut(s) 60
Msp20I TGGCCA 1 cut(s) 60
MwoI GCNNNNNNNGC 2 cut(s) 224, 286
NdeII GATC 4 cut(s) 104, 130, 138, 152
NheI GCTAGC 1 cut(s) 289
NmuCI GTSAC 1 cut(s) 85
PfeI GAWTC 2 cut(s) 51, 79
PspPI GGNCC 1 cut(s) 237
PsuI RGATCY 1 cut(s) 104
Sau3AI GATC 4 cut(s) 104, 130, 138, 152
Sau96I GGNCC 1 cut(s) 237
SetI ASST 7 cut(s) 25, 40, 186, 192, 229, 282, 291
SgeI CNNG 9 cut(s) 24, 95, 102, 197, 236, 246, 289, 302, 319
SinI GGWCC 1 cut(s) 237
SmlI CTYRAG 1 cut(s) 185
SmoI CTYRAG 1 cut(s) 185
Sse9I AATT 2 cut(s) 198, 241
SspMI CTAG 1 cut(s) 290
TaaI ACNGT 1 cut(s) 76
TaqI TCGA 1 cut(s) 223
TasI AATT 2 cut(s) 198, 241
TfiI GAWTC 2 cut(s) 51, 79
TseFI GTSAC 1 cut(s) 85
Tsp45I GTSAC 1 cut(s) 85
TspDTI ATGAA 2 cut(s) 43, 307
TspGWI ACGGA 1 cut(s) 48
VpaK11BI GGWCC 1 cut(s) 237
XspI CTAG 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.