RLG00000023972

Replication protein A 14 kDa subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013004
Physical Location & Seq
Forward (+)
30601461 .. 30605284
3824 bp
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UTR
Exon/CDS
Intron
RLM00000023972

Sequence Viewer

Length: 498 bp
ATGCCACCTCCAAATTCTCAGCTTTTGCTCACTGTTAGAATCCACAGTTATGCTGAGAAGAGAAAACCCTCAGATTCTTCTTCATCTTTTGCCGCGCTCTCCGTCGCTCAATCTTCCAAACTCAAGTTCTATTCTCGACTCAGTTTCTCCGGCGATCTCCTCAGATCACCAAGCGATATGGATACGTCAAACCCGTCTGTTTTTGTGAATGCAGAGCTGCTGCGGTTGTATATTGGAAGGCAGGTTCGGGCGTTGATTCAGGTTGTGCAATTGGATGCTGGATCTGTCATTGGCAAATCTACTGATGAAATTCAGTTAATTGTAAAGGGCACCCCACCCTTTCCTCTTACAAAATTTGTTGAGGTCATAGGTATTGCTGACACCGATAAATCTATCCAAGCTAGCATATGGAACAACTTTGGTGACACAATTGACACACATGCTTACAATCAGCTTTGCTTGCTCGCAAATGGAGAATTTAAGCACTTGTTCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
Rep_fac-A_3

Protein Analysis

166

Amino Acids

18.17

Weight (kDa)

7.86

Isoelectric Point (pI)

37.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep_fac-A_3 PF08661 60 - 161 6.5e-20 Replication factor A protein 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013004)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52630 AT3G52630 AT4G18590
fragaria_vesca FvH4_6g18910
malus_domestica MD12G1025300.v1.1 MD14G1024000.v1.1
prunus_persica Prupe.7G108800_v2.0.a1
pyrus_communis pycom12g02480
rosa_chinensis RchiOBHm_Chr3g0473901
rosa_laevigata RLG00000023972
rosa_multiflora Rmu_sc0002414.1_g000040
rosa_roxburghii Rroxscaffold_6G00407670
rosa_rugosa Rorug03G0136800
rosa_samantha Rh3AG187000 Rh3BG215600 Rh3CG212300 Rh3DG211500
rosa_wichuraiana Rw3G017120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 232
AccB1I GGYRCC 1 cut(s) 329
AccII CGCG 1 cut(s) 95
AciI CCGC 2 cut(s) 93, 223
AclWI GGATC 1 cut(s) 289
AcsI RAATTY 4 cut(s) 13, 309, 353, 476
AjuI GAANNNNNNNTTGG 2 cut(s) 110, 142
AloI GAACNNNNNNTCC 2 cut(s) 228, 260
AluBI AGCT 4 cut(s) 22, 217, 401, 454
AluI AGCT 4 cut(s) 22, 217, 401, 454
AlwI GGATC 1 cut(s) 289
ApeKI GCWGC 2 cut(s) 217, 220
ApoI RAATTY 4 cut(s) 13, 309, 353, 476
AspLEI GCGC 1 cut(s) 97
AsuHPI GGTGA 2 cut(s) 159, 434
AsuNHI GCTAGC 1 cut(s) 401
BaeGI GKGCMC 1 cut(s) 332
BanI GGYRCC 1 cut(s) 329
BbvI GCAGC 2 cut(s) 204, 207
BciVI GTATCC 1 cut(s) 175
BfaI CTAG 1 cut(s) 402
BfuAI ACCTGC 1 cut(s) 232
BfuI GTATCC 1 cut(s) 175
BisI GCNGC 3 cut(s) 93, 218, 221
BlsI GCNGC 3 cut(s) 94, 219, 222
BmiI GGNNCC 1 cut(s) 331
BmsI GCATC 1 cut(s) 265
BmtI GCTAGC 1 cut(s) 405
BpuEI CTTGAG 1 cut(s) 107
BseGI GGATG 1 cut(s) 280
BseMII CTCAG 5 cut(s) 32, 45, 84, 154, 175
BseRI GAGGAG 1 cut(s) 149
BseSI GKGCMC 1 cut(s) 332
BseXI GCAGC 2 cut(s) 204, 207
Bsh1236I CGCG 1 cut(s) 95
BshNI GGYRCC 1 cut(s) 329
BsiSI CCGG 1 cut(s) 150
BsmI GAATGC 1 cut(s) 214
Bsp1286I GDGCHC 1 cut(s) 332
Bsp143I GATC 3 cut(s) 154, 164, 281
BspACI CCGC 2 cut(s) 93, 223
BspCNI CTCAG 5 cut(s) 31, 46, 83, 153, 174
BspFNI CGCG 1 cut(s) 95
BspLI GGNNCC 1 cut(s) 331
BspMI ACCTGC 1 cut(s) 232
BspOI GCTAGC 1 cut(s) 405
BspPI GGATC 1 cut(s) 289
BspT107I GGYRCC 1 cut(s) 329
BssMI GATC 3 cut(s) 154, 164, 281
Bst4CI ACNGT 2 cut(s) 34, 47
Bst6I CTCTTC 1 cut(s) 53
BstC8I GCNNGC 3 cut(s) 403, 461, 465
BstDEI CTNAG 5 cut(s) 18, 54, 70, 140, 161
BstF5I GGATG 1 cut(s) 280
BstFNI CGCG 1 cut(s) 95
BstHHI GCGC 1 cut(s) 97
BstKTI GATC 3 cut(s) 157, 167, 284
BstMBI GATC 3 cut(s) 154, 164, 281
BstMWI GCNNNNNNNGC 1 cut(s) 460
BstNSI RCATGY 1 cut(s) 443
BstSLI GKGCMC 1 cut(s) 332
BstUI CGCG 1 cut(s) 95
BstV1I GCAGC 2 cut(s) 204, 207
BstX2I RGATCY 1 cut(s) 281
BstYI RGATCY 1 cut(s) 281
BsuI GTATCC 1 cut(s) 175
BtsCI GGATG 1 cut(s) 280
BtsIMutI CAGTG 1 cut(s) 30
BveI ACCTGC 1 cut(s) 232
Cac8I GCNNGC 3 cut(s) 403, 461, 465
CfoI GCGC 1 cut(s) 97
CviAII CATG 1 cut(s) 440
CviJI RGCY 4 cut(s) 22, 217, 401, 454
CviKI_1 RGCY 4 cut(s) 22, 217, 401, 454
DdeI CTNAG 5 cut(s) 18, 54, 70, 140, 161
DpnI GATC 3 cut(s) 156, 166, 283
DpnII GATC 3 cut(s) 154, 164, 281
Eam1104I CTCTTC 1 cut(s) 53
EarI CTCTTC 1 cut(s) 53
FaeI CATG 1 cut(s) 443
FaiI YATR 7 cut(s) 51, 179, 231, 368, 407, 409, 441
FatI CATG 1 cut(s) 439
FauNDI CATATG 1 cut(s) 407
Fnu4HI GCNGC 3 cut(s) 93, 218, 221
FokI GGATG 1 cut(s) 287
Fsp4HI GCNGC 3 cut(s) 93, 218, 221
FspBI CTAG 1 cut(s) 402
GlaI GCGC 1 cut(s) 96
GluI GCNGC 3 cut(s) 93, 218, 221
HapII CCGG 1 cut(s) 150
HhaI GCGC 1 cut(s) 97
Hin1II CATG 1 cut(s) 443
Hin6I GCGC 1 cut(s) 95
HinP1I GCGC 1 cut(s) 95
HinfI GANTC 4 cut(s) 39, 74, 138, 256
HpaII CCGG 1 cut(s) 150
HphI GGTGA 2 cut(s) 159, 434
Hpy188I TCNGA 3 cut(s) 73, 164, 497
Hpy188III TCNNGA 1 cut(s) 135
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 1 cut(s) 231
HpyCH4III ACNGT 2 cut(s) 34, 47
HpyCH4IV ACGT 1 cut(s) 185
HpyCH4V TGCA 2 cut(s) 212, 268
HpyF10VI GCNNNNNNNGC 1 cut(s) 460
HpyF3I CTNAG 5 cut(s) 18, 54, 70, 140, 161
HpySE526I ACGT 1 cut(s) 185
Hsp92II CATG 1 cut(s) 443
HspAI GCGC 1 cut(s) 95
Kzo9I GATC 3 cut(s) 154, 164, 281
LpnPI CCDG 4 cut(s) 163, 227, 245, 264
Lsp1109I GCAGC 2 cut(s) 204, 207
LweI GCATC 1 cut(s) 265
MaeI CTAG 1 cut(s) 402
MaeII ACGT 1 cut(s) 185
MaeIII GTNAC 1 cut(s) 422
MalI GATC 3 cut(s) 156, 166, 283
MboI GATC 3 cut(s) 154, 164, 281
MboII GAAGA 4 cut(s) 69, 70, 72, 105
MfeI CAATTG 2 cut(s) 269, 429
MflI RGATCY 1 cut(s) 281
MhlI GDGCHC 1 cut(s) 332
MluCI AATT 7 cut(s) 13, 269, 309, 318, 353, 429, 476
MlyI GAGTC 1 cut(s) 132
MnlI CCTC 5 cut(s) 18, 79, 170, 354, 355
MseI TTAA 2 cut(s) 317, 480
MslI CAYNNNNRTG 1 cut(s) 48
MspI CCGG 1 cut(s) 150
MunI CAATTG 2 cut(s) 269, 429
Mva1269I GAATGC 1 cut(s) 214
MvnI CGCG 1 cut(s) 95
MwoI GCNNNNNNNGC 1 cut(s) 460
NdeI CATATG 1 cut(s) 407
NdeII GATC 3 cut(s) 154, 164, 281
NheI GCTAGC 1 cut(s) 401
NlaIII CATG 1 cut(s) 443
NlaIV GGNNCC 1 cut(s) 331
NmuCI GTSAC 1 cut(s) 422
NspI RCATGY 1 cut(s) 443
PcsI WCGNNNNNNNCGW 1 cut(s) 191
PctI GAATGC 1 cut(s) 214
PfeI GAWTC 3 cut(s) 39, 74, 256
PkrI GCNGC 3 cut(s) 94, 219, 222
PleI GAGTC 1 cut(s) 132
PpsI GAGTC 1 cut(s) 132
PspN4I GGNNCC 1 cut(s) 331
PsuI RGATCY 1 cut(s) 281
RseI CAYNNNNRTG 1 cut(s) 48
SaqAI TTAA 2 cut(s) 317, 480
SatI GCNGC 3 cut(s) 93, 218, 221
Sau3AI GATC 3 cut(s) 154, 164, 281
SchI GAGTC 1 cut(s) 132
SduI GDGCHC 1 cut(s) 332
SfaNI GCATC 1 cut(s) 265
SmiMI CAYNNNNRTG 1 cut(s) 48
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 7 cut(s) 13, 269, 309, 318, 353, 429, 476
SsiI CCGC 2 cut(s) 93, 223
SspMI CTAG 1 cut(s) 402
TaaI ACNGT 2 cut(s) 34, 47
TaiI ACGT 1 cut(s) 188
TaqI TCGA 1 cut(s) 136
TasI AATT 7 cut(s) 13, 269, 309, 318, 353, 429, 476
TauI GCSGC 1 cut(s) 95
TfiI GAWTC 3 cut(s) 39, 74, 256
Tru1I TTAA 2 cut(s) 317, 480
Tru9I TTAA 2 cut(s) 317, 480
TscAI CASTG 1 cut(s) 37
TseFI GTSAC 1 cut(s) 422
TseI GCWGC 2 cut(s) 217, 220
Tsp45I GTSAC 1 cut(s) 422
TspDTI ATGAA 3 cut(s) 72, 321, 481
TspGWI ACGGA 1 cut(s) 91
TspRI CASTG 1 cut(s) 37
XapI RAATTY 4 cut(s) 13, 309, 353, 476
XceI RCATGY 1 cut(s) 443
XspI CTAG 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.