MD14G1024000.v1.1

Replication protein A 14 kDa subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013004
Physical Location & Seq
Reverse (-)
2161268 .. 2163717
2450 bp
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UTR
Exon/CDS
Intron
MD14G1024000.v1.1.491

Sequence Viewer

Length: 342 bp
ATGGGTTGCAGATTATGTGATATGGATACATCAAATCCGGCAGTTTTTGTCAATGCAGAGTTGTTGCGACTGTATGTTGGAAGGCGGGCTCGGGCACTGATTCAGGTGGTGCGAACTGAGGGTGGAACTGTCATTGGAAAATCTACTGATGGAAAGGAGCTAGTTGTGAAGGGCAACCCGCCGGTTCCTCTTACAAAATTTGTTGAGGTCATAGGCATTGCTGACAGTGATAAGTCCATCCATGCTGAGATATGGAACAACTTTGGAGAGACAATTGACACATCTTCTTACAATCAGCTCTGTCAGCTTGCAAATGGAGAATATAAACAGTTGTTCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
Rep_fac-A_3

Protein Analysis

114

Amino Acids

12.42

Weight (kDa)

5.77

Isoelectric Point (pI)

21.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep_fac-A_3 PF08661 8 - 107 2.3e-21 Replication factor A protein 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013004)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52630 AT3G52630 AT4G18590
fragaria_vesca FvH4_6g18910
malus_domestica MD12G1025300.v1.1 MD14G1024000.v1.1
prunus_persica Prupe.7G108800_v2.0.a1
pyrus_communis pycom12g02480
rosa_chinensis RchiOBHm_Chr3g0473901
rosa_laevigata RLG00000023972
rosa_multiflora Rmu_sc0002414.1_g000040
rosa_roxburghii Rroxscaffold_6G00407670
rosa_rugosa Rorug03G0136800
rosa_samantha Rh3AG187000 Rh3BG215600 Rh3CG212300 Rh3DG211500
rosa_wichuraiana Rw3G017120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 85, 179
AcsI RAATTY 1 cut(s) 197
AluBI AGCT 3 cut(s) 160, 298, 307
AluI AGCT 3 cut(s) 160, 298, 307
Alw26I GTCTC 1 cut(s) 263
Ama87I CYCGRG 1 cut(s) 90
ApoI RAATTY 1 cut(s) 197
AvaI CYCGRG 1 cut(s) 90
BaeGI GKGCMC 1 cut(s) 97
BanII GRGCYC 1 cut(s) 91
BccI CCATC 2 cut(s) 143, 245
BciVI GTATCC 1 cut(s) 19
BcoDI GTCTC 1 cut(s) 263
BfaI CTAG 1 cut(s) 161
BfuI GTATCC 1 cut(s) 19
BmeT110I CYCGRG 1 cut(s) 90
BmiI GGNNCC 1 cut(s) 186
Bse118I RCCGGY 1 cut(s) 181
Bse3DI GCAATG 1 cut(s) 216
BseGI GGATG 1 cut(s) 237
BseMI GCAATG 1 cut(s) 216
BseMII CTCAG 2 cut(s) 108, 237
BseSI GKGCMC 1 cut(s) 97
BsiHKCI CYCGRG 1 cut(s) 90
BsiSI CCGG 2 cut(s) 38, 182
BsmAI GTCTC 1 cut(s) 263
BsoBI CYCGRG 1 cut(s) 90
Bsp1286I GDGCHC 2 cut(s) 91, 97
BspACI CCGC 2 cut(s) 85, 179
BspCNI CTCAG 2 cut(s) 109, 238
BspLI GGNNCC 1 cut(s) 186
BsrDI GCAATG 1 cut(s) 216
BsrFI RCCGGY 1 cut(s) 181
BssAI RCCGGY 1 cut(s) 181
Bst4CI ACNGT 4 cut(s) 72, 130, 227, 330
BstC8I GCNNGC 2 cut(s) 87, 309
BstDEI CTNAG 2 cut(s) 117, 246
BstF5I GGATG 1 cut(s) 237
BstMAI GTCTC 1 cut(s) 263
BstMWI GCNNNNNNNGC 1 cut(s) 304
BstSLI GKGCMC 1 cut(s) 97
BsuI GTATCC 1 cut(s) 19
BtsCI GGATG 1 cut(s) 237
BtsIMutI CAGTG 2 cut(s) 95, 232
Cac8I GCNNGC 2 cut(s) 87, 309
Cfr10I RCCGGY 1 cut(s) 181
CviAII CATG 1 cut(s) 242
CviJI RGCY 4 cut(s) 89, 160, 298, 307
CviKI_1 RGCY 4 cut(s) 89, 160, 298, 307
DdeI CTNAG 2 cut(s) 117, 246
Eco24I GRGCYC 1 cut(s) 91
Eco88I CYCGRG 1 cut(s) 90
EcoT38I GRGCYC 1 cut(s) 91
FaeI CATG 1 cut(s) 245
FaiI YATR 7 cut(s) 16, 23, 75, 212, 243, 253, 324
FatI CATG 1 cut(s) 241
FauI CCCGC 2 cut(s) 78, 186
FokI GGATG 1 cut(s) 224
FriOI GRGCYC 1 cut(s) 91
FspBI CTAG 1 cut(s) 161
HapII CCGG 2 cut(s) 38, 182
Hin1II CATG 1 cut(s) 245
HinfI GANTC 1 cut(s) 100
HpaII CCGG 2 cut(s) 38, 182
Hpy188I TCNGA 1 cut(s) 341
HpyAV CCTTC 2 cut(s) 75, 163
HpyCH4III ACNGT 4 cut(s) 72, 130, 227, 330
HpyCH4V TGCA 3 cut(s) 9, 56, 311
HpyF10VI GCNNNNNNNGC 1 cut(s) 304
HpyF3I CTNAG 2 cut(s) 117, 246
Hsp92II CATG 1 cut(s) 245
LmnI GCTCC 1 cut(s) 157
LpnPI CCDG 3 cut(s) 51, 89, 195
MaeI CTAG 1 cut(s) 161
MboII GAAGA 1 cut(s) 276
MfeI CAATTG 1 cut(s) 273
MhlI GDGCHC 2 cut(s) 91, 97
MluCI AATT 2 cut(s) 197, 273
MmeI TCCRAC 1 cut(s) 58
MnlI CCTC 3 cut(s) 112, 198, 199
MspI CCGG 2 cut(s) 38, 182
MunI CAATTG 1 cut(s) 273
MwoI GCNNNNNNNGC 1 cut(s) 304
NlaIII CATG 1 cut(s) 245
NlaIV GGNNCC 1 cut(s) 186
PfeI GAWTC 1 cut(s) 100
PspN4I GGNNCC 1 cut(s) 186
SduI GDGCHC 2 cut(s) 91, 97
SetI ASST 5 cut(s) 108, 162, 210, 300, 309
Sse9I AATT 2 cut(s) 197, 273
SsiI CCGC 2 cut(s) 85, 179
SspMI CTAG 1 cut(s) 161
TaaI ACNGT 4 cut(s) 72, 130, 227, 330
TasI AATT 2 cut(s) 197, 273
TfiI GAWTC 1 cut(s) 100
TscAI CASTG 2 cut(s) 102, 232
TspDTI ATGAA 1 cut(s) 325
TspRI CASTG 2 cut(s) 102, 232
XapI RAATTY 1 cut(s) 197
XspI CTAG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.