MD12G1025300.v1.1

Replication protein A 14 kDa subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013004
Physical Location & Seq
Reverse (-)
2698484 .. 2700560
2077 bp
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UTR
Exon/CDS
Intron
MD12G1025300.v1.1.491

Sequence Viewer

Length: 333 bp
ATGCCTCCCCGTCGTCGAGATATGAATCCTGCAGTTTTTGTCAATGCAGAGTTGTTGCGATCGTATGTTGGAAGTCGGGTTCGGGCACTGATTCAGGTGGTGCGAGTTGAGGGTGAAACTATCATTGGAAATTCTACTGATGAAAGCCAGCTAGTTGTGAAGGGCATCCCAACGTTTCCTCTTACAAAATTTGTTGAGGTCATAGGCCTTGCTGACAGTGATACGTCCATCCAGGCTGACATGTGGACCAACCTCGGAGAGACAGCTGATACATACACTTACAATCAGCTCTGTCAGATTGTAAATGGGGAATATAAAATCTTGTTCGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
Rep_fac-A_3

Protein Analysis

111

Amino Acids

12.3

Weight (kDa)

4.82

Isoelectric Point (pI)

25.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep_fac-A_3 PF08661 10 - 106 3.6e-21 Replication factor A protein 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013004)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52630 AT3G52630 AT4G18590
fragaria_vesca FvH4_6g18910
malus_domestica MD12G1025300.v1.1 MD14G1024000.v1.1
prunus_persica Prupe.7G108800_v2.0.a1
pyrus_communis pycom12g02480
rosa_chinensis RchiOBHm_Chr3g0473901
rosa_laevigata RLG00000023972
rosa_multiflora Rmu_sc0002414.1_g000040
rosa_roxburghii Rroxscaffold_6G00407670
rosa_rugosa Rorug03G0136800
rosa_samantha Rh3AG187000 Rh3BG215600 Rh3CG212300 Rh3DG211500
rosa_wichuraiana Rw3G017120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 173
AcsI RAATTY 2 cut(s) 130, 188
AflIII ACRYGT 1 cut(s) 240
AjnI CCWGG 1 cut(s) 231
AjuI GAANNNNNNNTTGG 2 cut(s) 108, 140
AloI GAACNNNNNNTCC 2 cut(s) 63, 95
AluBI AGCT 3 cut(s) 151, 266, 289
AluI AGCT 3 cut(s) 151, 266, 289
Alw26I GTCTC 1 cut(s) 254
AoxI GGCC 1 cut(s) 205
ApoI RAATTY 2 cut(s) 130, 188
AspS9I GGNCC 1 cut(s) 246
AsuHPI GGTGA 1 cut(s) 125
AvaII GGWCC 1 cut(s) 246
BaeGI GKGCMC 1 cut(s) 88
BccI CCATC 1 cut(s) 236
BciT130I CCWGG 1 cut(s) 233
BcoDI GTCTC 1 cut(s) 254
BfaI CTAG 1 cut(s) 152
BfmI CTRYAG 1 cut(s) 30
Bme1390I CCNGG 1 cut(s) 233
Bme18I GGWCC 1 cut(s) 246
BmgT120I GGNCC 1 cut(s) 246
BmrFI CCNGG 1 cut(s) 233
BmsI GCATC 1 cut(s) 174
BsaBI GATNNNNATC 1 cut(s) 24
BsaJI CCNNGG 1 cut(s) 253
Bse8I GATNNNNATC 1 cut(s) 24
BseBI CCWGG 1 cut(s) 233
BseDI CCNNGG 1 cut(s) 253
BseGI GGATG 2 cut(s) 165, 228
BseJI GATNNNNATC 1 cut(s) 24
BseSI GKGCMC 1 cut(s) 88
Bsh1285I CGRYCG 1 cut(s) 62
BshFI GGCC 1 cut(s) 207
BsiEI CGRYCG 1 cut(s) 62
BsmAI GTCTC 1 cut(s) 254
BsnI GGCC 1 cut(s) 207
Bsp1286I GDGCHC 1 cut(s) 88
Bsp143I GATC 1 cut(s) 59
BspANI GGCC 1 cut(s) 207
BspMAI CTGCAG 1 cut(s) 34
BssECI CCNNGG 1 cut(s) 253
BssMI GATC 1 cut(s) 59
Bst2UI CCWGG 1 cut(s) 233
Bst4CI ACNGT 1 cut(s) 218
BstC8I GCNNGC 1 cut(s) 149
BstF5I GGATG 2 cut(s) 165, 228
BstKTI GATC 1 cut(s) 62
BstMAI GTCTC 1 cut(s) 254
BstMBI GATC 1 cut(s) 59
BstMCI CGRYCG 1 cut(s) 62
BstNI CCWGG 1 cut(s) 233
BstNSI RCATGY 1 cut(s) 244
BstSCI CCNGG 1 cut(s) 231
BstSFI CTRYAG 1 cut(s) 30
BstSLI GKGCMC 1 cut(s) 88
BsuRI GGCC 1 cut(s) 207
BtsCI GGATG 2 cut(s) 165, 228
BtsIMutI CAGTG 2 cut(s) 86, 223
Cac8I GCNNGC 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 246
CviAII CATG 1 cut(s) 241
CviJI RGCY 6 cut(s) 147, 151, 207, 236, 266, 289
CviKI_1 RGCY 6 cut(s) 147, 151, 207, 236, 266, 289
DpnI GATC 1 cut(s) 61
DpnII GATC 1 cut(s) 59
Eco147I AGGCCT 1 cut(s) 207
Eco47I GGWCC 1 cut(s) 246
EcoRII CCWGG 1 cut(s) 231
FaeI CATG 1 cut(s) 244
FaiI YATR 6 cut(s) 23, 66, 203, 242, 274, 315
FatI CATG 1 cut(s) 240
FokI GGATG 2 cut(s) 152, 215
FspBI CTAG 1 cut(s) 152
HaeIII GGCC 1 cut(s) 207
Hin1II CATG 1 cut(s) 244
HinfI GANTC 2 cut(s) 25, 91
HphI GGTGA 1 cut(s) 125
Hpy166II GTNNAC 1 cut(s) 246
Hpy188I TCNGA 3 cut(s) 257, 297, 332
Hpy188III TCNNGA 1 cut(s) 17
Hpy8I GTNNAC 1 cut(s) 246
Hpy99I CGWCG 2 cut(s) 15, 18
HpyAV CCTTC 1 cut(s) 154
HpyCH4III ACNGT 1 cut(s) 218
HpyCH4IV ACGT 2 cut(s) 173, 224
HpyCH4V TGCA 2 cut(s) 32, 47
HpySE526I ACGT 2 cut(s) 173, 224
Hsp92II CATG 1 cut(s) 244
Kzo9I GATC 1 cut(s) 59
LpnPI CCDG 5 cut(s) 42, 80, 161, 218, 245
LweI GCATC 1 cut(s) 174
MaeI CTAG 1 cut(s) 152
MaeII ACGT 2 cut(s) 173, 224
MalI GATC 1 cut(s) 61
MboI GATC 1 cut(s) 59
MhlI GDGCHC 1 cut(s) 88
MluCI AATT 2 cut(s) 130, 188
MmeI TCCRAC 1 cut(s) 49
MnlI CCTC 5 cut(s) 15, 103, 189, 190, 263
MspA1I CMGCKG 1 cut(s) 266
MspR9I CCNGG 1 cut(s) 233
MvaI CCWGG 1 cut(s) 233
NdeII GATC 1 cut(s) 59
NlaIII CATG 1 cut(s) 244
NspI RCATGY 1 cut(s) 244
PceI AGGCCT 1 cut(s) 207
PciI ACATGT 1 cut(s) 240
PfeI GAWTC 2 cut(s) 25, 91
Ple19I CGATCG 1 cut(s) 62
PscI ACATGT 1 cut(s) 240
Psp1406I AACGTT 1 cut(s) 173
Psp6I CCWGG 1 cut(s) 231
PspGI CCWGG 1 cut(s) 231
PspPI GGNCC 1 cut(s) 246
PstI CTGCAG 1 cut(s) 34
PvuI CGATCG 1 cut(s) 62
PvuII CAGCTG 1 cut(s) 266
Sau3AI GATC 1 cut(s) 59
Sau96I GGNCC 1 cut(s) 246
ScrFI CCNGG 1 cut(s) 233
SduI GDGCHC 1 cut(s) 88
SetI ASST 8 cut(s) 99, 153, 176, 201, 227, 255, 268, 291
SfaNI GCATC 1 cut(s) 174
SfcI CTRYAG 1 cut(s) 30
SinI GGWCC 1 cut(s) 246
Sse9I AATT 2 cut(s) 130, 188
SseBI AGGCCT 1 cut(s) 207
SspMI CTAG 1 cut(s) 152
StuI AGGCCT 1 cut(s) 207
StyD4I CCNGG 1 cut(s) 231
TaaI ACNGT 1 cut(s) 218
TaiI ACGT 2 cut(s) 176, 227
TaqI TCGA 1 cut(s) 16
TasI AATT 2 cut(s) 130, 188
TfiI GAWTC 2 cut(s) 25, 91
TscAI CASTG 2 cut(s) 93, 223
TspDTI ATGAA 2 cut(s) 38, 156
TspRI CASTG 2 cut(s) 93, 223
VpaK11BI GGWCC 1 cut(s) 246
XapI RAATTY 2 cut(s) 130, 188
XceI RCATGY 1 cut(s) 244
XspI CTAG 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.