AT3G53130

cytochrome P450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009299
Physical Location & Seq
Forward (+)
19692757 .. 19697542
4786 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G53130.1

Sequence Viewer

Length: 1620 bp
ATGGAGTCTTCACTCTTTTCTCCATCTTCCTCTTCTTACTCTTCTCTCTTCACTGCAAAACCTACGCGTCTTTTATCACCAAAACCCAAATTCACATTCTCCATCAGATCCTCCATTGAGAAACCCAAACCCAAACTCGAGACCAATTCATCGAAATCCCAATCATGGGTCAGTCCCGATTGGCTCACAACACTCACTCGTACCCTTTCCTCAGGAAAAAACGACGAGTCAGGTATACCAATCGCGAACGCGAAGCTCGACGATGTCGCTGATCTCCTCGGAGGTGCTCTCTTCTTACCTCTCTACAAATGGATGAATGAGTACGGACCCATTTACCGTCTCGCTGCTGGTCCTCGTAATTTCGTAATTGTGAGCGACCCAGCGATAGCTAAACATGTTTTGAGGAATTATCCAAAGTACGCTAAAGGCTTAGTCGCTGAAGTCTCTGAATTTCTATTTGGTTCGGGTTTCGCTATCGCTGAAGGACCTCTTTGGACAGCGAGGCGTAGAGCGGTGGTTCCATCGCTTCACAGGAGGTATTTGTCTGTGATTGTGGAGAGAGTATTCTGCAAATGTGCAGAGAGGCTTGTTGAGAAGTTGCAGCCTTATGCAGAAGACGGAAGTGCTGTGAATATGGAAGCGAAGTTCTCTCAGATGACACTTGATGTCATTGGGTTGTCTCTTTTTAACTACAATTTCGATTCTTTGACTACTGATAGTCCTGTCATTGAAGCTGTTTACACTGCTCTTAAAGAAGCTGAGCTTCGTTCTACTGATCTTCTGCCATATTGGAAGATCGATGCATTGTGTAAGATAGTCCCGAGACAGGTGAAAGCTGAAAAGGCTGTAACTTTGATAAGGGAAACTGTTGAAGACCTTATTGCTAAGTGTAAAGAAATTGTCGAAAGAGAAGGCGAAAGAATCAATGATGAGGAGTATGTAAATGATGCTGACCCAAGTATCCTGCGTTTCTTGCTTGCAAGCAGAGAAGAGGTATCAAGTGTGCAGTTACGGGATGATCTTCTCTCAATGCTCGTAGCGGGTCATGAAACCACTGGATCTGTCCTCACTTGGACACTTTATCTCCTAAGTAAGAACTCATCTGCATTAAGGAAAGCACAAGAAGAAGTAGACAGAGTGTTAGAAGGAAGAAACCCGGCTTTCGAGGATATAAAGGAGTTGAAGTACATCACTCGTTGTATAAACGAGTCAATGCGTCTCTATCCTCATCCTCCTGTCTTGATAAGAAGAGCTCAAGTTCCTGACATTCTTCCTGGGAACTATAAGGTCAATACCGGACAAGACATTATGATTTCAGTCTATAACATCCATCGTTCTTCCGAGGTATGGGAAAAAGCTGAGGAATTTCTGCCTGAACGATTCGACATAGATGGCGCAATCCCTAACGAAACAAACACTGATTTCAAATTCATCCCATTCAGTGGAGGGCCTAGAAAATGTGTAGGCGATCAGTTTGCATTGATGGAGGCAATTGTGGCACTCGCGGTGTTTCTTCAGCGGTTAAACGTTGAGCTGGTTCCTGATCAGACCATTAGCATGACCACAGGAGCAACCATACACACCACCAATGGATTGTATATGAAGGTGAGCCAAAGGTAA

Protein Analysis

539

Amino Acids

60.55

Weight (kDa)

6.04

Isoelectric Point (pI)

39.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 102 - 522 2.2e-82 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1442
AccBSI CCGCTC 1 cut(s) 512
AccI GTMKAC 2 cut(s) 235, 1131
AccII CGCG 4 cut(s) 67, 245, 251, 1505
AciI CCGC 4 cut(s) 512, 1040, 1505, 1519
AclI AACGTT 1 cut(s) 1527
AclWI GGATC 2 cut(s) 102, 1066
AcsI RAATTY 4 cut(s) 89, 449, 1364, 1427
AcuI CTGAAG 3 cut(s) 459, 501, 1499
AfaI GTAC 4 cut(s) 202, 323, 419, 1187
AfiI CCNNNNNNNGG 5 cut(s) 165, 166, 826, 1347, 1442
AflIII ACRYGT 2 cut(s) 65, 394
AgsI TTSAA 4 cut(s) 731, 872, 1183, 1426
AjnI CCWGG 1 cut(s) 1273
AjuI GAANNNNNNNTTGG 4 cut(s) 80, 112, 441, 473
AloI GAACNNNNNNTCC 2 cut(s) 629, 661
AluBI AGCT 9 cut(s) 256, 389, 734, 758, 763, 836, 1253, 1358, 1534
AluI AGCT 9 cut(s) 256, 389, 734, 758, 763, 836, 1253, 1358, 1534
Alw21I GWGCWC 2 cut(s) 289, 1255
Alw26I GTCTC 6 cut(s) 134, 344, 448, 684, 817, 1223
AlwI GGATC 2 cut(s) 102, 1066
Ama87I CYCGRG 2 cut(s) 137, 820
AoxI GGCC 1 cut(s) 1448
ApeKI GCWGC 2 cut(s) 344, 601
ApoI RAATTY 4 cut(s) 89, 449, 1364, 1427
AspLEI GCGC 1 cut(s) 1397
AspS9I GGNCC 4 cut(s) 326, 350, 485, 1448
AsuC2I CCSGG 1 cut(s) 1157
AsuHPI GGTGA 3 cut(s) 69, 841, 1618
AvaI CYCGRG 2 cut(s) 137, 820
AvaII GGWCC 3 cut(s) 326, 350, 485
AxyI CCTNAGG 1 cut(s) 211
BaeI ACNNNNGTAYC 2 cut(s) 943, 976
BanII GRGCYC 1 cut(s) 1255
BbsI GAAGAC 2 cut(s) 621, 879
Bbv12I GWGCWC 2 cut(s) 289, 1255
BbvCI CCTCAGC 1 cut(s) 1359
BbvI GCAGC 2 cut(s) 331, 613
BccI CCATC 6 cut(s) 31, 110, 529, 1338, 1385, 1477
BcgI CGANNNNNNTGC 2 cut(s) 1457, 1491
BciT130I CCWGG 1 cut(s) 1275
BciVI GTATCC 1 cut(s) 971
BclI TGATCA 1 cut(s) 1543
BcnI CCSGG 1 cut(s) 1157
BcoDI GTCTC 6 cut(s) 134, 344, 448, 684, 817, 1223
BfaI CTAG 1 cut(s) 1452
BfuI GTATCC 1 cut(s) 971
BisI GCNGC 2 cut(s) 345, 602
BlpI GCTNAGC 1 cut(s) 759
BlsI GCNGC 2 cut(s) 346, 603
Bme1390I CCNGG 2 cut(s) 1157, 1275
Bme18I GGWCC 3 cut(s) 326, 350, 485
BmeT110I CYCGRG 2 cut(s) 137, 820
BmgT120I GGNCC 4 cut(s) 326, 350, 485, 1448
BmiI GGNNCC 3 cut(s) 328, 519, 1539
BmrFI CCNGG 2 cut(s) 1157, 1275
BmsI GCATC 2 cut(s) 790, 937
BpiI GAAGAC 2 cut(s) 621, 879
BplI GAGNNNNNCTC 2 cut(s) 273, 305
Bpu10I CCTNAGC 1 cut(s) 1359
Bpu1102I GCTNAGC 1 cut(s) 759
BpuEI CTTGAG 1 cut(s) 1239
BpuMI CCSGG 1 cut(s) 1157
Bsa29I ATCGAT 1 cut(s) 798
BsaI GGTCTC 1 cut(s) 134
BsaJI CCNNGG 3 cut(s) 277, 1274, 1341
BsaWI WCCGGW 1 cut(s) 1295
BsaXI ACNNNNNCTCC 4 cut(s) 1068, 1098, 1169, 1199
Bsc4I CCNNNNNNNGG 5 cut(s) 165, 166, 826, 1347, 1442
Bse1I ACTGG 1 cut(s) 1060
Bse21I CCTNAGG 1 cut(s) 211
BseBI CCWGG 1 cut(s) 1275
BseCI ATCGAT 1 cut(s) 798
BseDI CCNNGG 3 cut(s) 277, 1274, 1341
BseGI GGATG 5 cut(s) 318, 1021, 1228, 1326, 1431
BseLI CCNNNNNNNGG 5 cut(s) 165, 166, 826, 1347, 1442
BseMII CTCAG 4 cut(s) 225, 665, 750, 1350
BseNI ACTGG 1 cut(s) 1060
BseRI GAGGAG 2 cut(s) 266, 947
BseXI GCAGC 2 cut(s) 331, 613
BseYI CCCAGC 1 cut(s) 379
BsgI GTGCAG 2 cut(s) 597, 1025
Bsh1236I CGCG 4 cut(s) 67, 245, 251, 1505
BshFI GGCC 1 cut(s) 1450
BshVI ATCGAT 1 cut(s) 798
BsiHKAI GWGCWC 2 cut(s) 289, 1255
BsiHKCI CYCGRG 2 cut(s) 137, 820
BsiSI CCGG 2 cut(s) 1157, 1296
BslFI GGGAC 2 cut(s) 159, 803
BslI CCNNNNNNNGG 5 cut(s) 165, 166, 826, 1347, 1442
BsmAI GTCTC 6 cut(s) 134, 344, 448, 684, 817, 1223
BsmBI CGTCTC 2 cut(s) 344, 1223
BsmFI GGGAC 2 cut(s) 159, 803
BsnI GGCC 1 cut(s) 1450
Bso31I GGTCTC 1 cut(s) 134
BsoBI CYCGRG 2 cut(s) 137, 820
Bsp1286I GDGCHC 2 cut(s) 289, 1255
Bsp143I GATC 8 cut(s) 107, 271, 775, 795, 1018, 1058, 1468, 1543
Bsp1720I GCTNAGC 1 cut(s) 759
Bsp68I TCGCGA 1 cut(s) 245
BspACI CCGC 4 cut(s) 512, 1040, 1505, 1519
BspANI GGCC 1 cut(s) 1450
BspCNI CTCAG 4 cut(s) 224, 664, 751, 1351
BspDI ATCGAT 1 cut(s) 798
BspFNI CGCG 4 cut(s) 67, 245, 251, 1505
BspHI TCATGA 1 cut(s) 1045
BspLI GGNNCC 3 cut(s) 328, 519, 1539
BspPI GGATC 2 cut(s) 102, 1066
BspQI GCTCTTC 1 cut(s) 1243
BspTNI GGTCTC 1 cut(s) 134
BsrBI CCGCTC 1 cut(s) 512
BsrI ACTGG 1 cut(s) 1060
BssECI CCNNGG 3 cut(s) 277, 1274, 1341
BssMI GATC 8 cut(s) 107, 271, 775, 795, 1018, 1058, 1468, 1543
BssNAI GTATAC 1 cut(s) 236
Bst1107I GTATAC 1 cut(s) 236
Bst2UI CCWGG 1 cut(s) 1275
Bst4CI ACNGT 2 cut(s) 338, 868
Bst6I CTCTTC 6 cut(s) 37, 46, 53, 296, 984, 1243
BstC8I GCNNGC 2 cut(s) 978, 982
BstDEI CTNAG 7 cut(s) 211, 430, 651, 759, 885, 1088, 1359
BstF5I GGATG 5 cut(s) 318, 1021, 1228, 1326, 1431
BstFNI CGCG 4 cut(s) 67, 245, 251, 1505
BstHHI GCGC 1 cut(s) 1397
BstKTI GATC 8 cut(s) 110, 274, 778, 798, 1021, 1061, 1471, 1546
BstMAI GTCTC 6 cut(s) 134, 344, 448, 684, 817, 1223
BstMBI GATC 8 cut(s) 107, 271, 775, 795, 1018, 1058, 1468, 1543
BstMWI GCNNNNNNNGC 3 cut(s) 842, 973, 1496
BstNI CCWGG 1 cut(s) 1275
BstNSI RCATGY 1 cut(s) 398
BstSCI CCNGG 2 cut(s) 1155, 1273
BstUI CGCG 4 cut(s) 67, 245, 251, 1505
BstV1I GCAGC 2 cut(s) 331, 613
BstV2I GAAGAC 2 cut(s) 621, 879
BstX2I RGATCY 2 cut(s) 107, 1058
BstYI RGATCY 2 cut(s) 107, 1058
BstZ17I GTATAC 1 cut(s) 236
Bsu15I ATCGAT 1 cut(s) 798
Bsu36I CCTNAGG 1 cut(s) 211
BsuI GTATCC 1 cut(s) 971
BsuRI GGCC 1 cut(s) 1450
BsuTUI ATCGAT 1 cut(s) 798
BtgZI GCGATG 1 cut(s) 507
BtsCI GGATG 5 cut(s) 318, 1021, 1228, 1326, 1431
BtsI GCAGTG 2 cut(s) 51, 741
BtsIMutI CAGTG 5 cut(s) 51, 741, 1053, 1416, 1447
BtuMI TCGCGA 1 cut(s) 245
Cac8I GCNNGC 2 cut(s) 978, 982
CciI TCATGA 1 cut(s) 1045
CfoI GCGC 1 cut(s) 1397
Cfr13I GGNCC 4 cut(s) 326, 350, 485, 1448
ClaI ATCGAT 1 cut(s) 798
CseI GACGC 2 cut(s) 56, 1205
Csp6I GTAC 4 cut(s) 201, 322, 418, 1186
CviAII CATG 4 cut(s) 165, 395, 1046, 1558
CviQI GTAC 4 cut(s) 201, 322, 418, 1186
DdeI CTNAG 7 cut(s) 211, 430, 651, 759, 885, 1088, 1359
DpnI GATC 8 cut(s) 109, 273, 777, 797, 1020, 1060, 1470, 1545
DpnII GATC 8 cut(s) 107, 271, 775, 795, 1018, 1058, 1468, 1543
Eam1104I CTCTTC 6 cut(s) 37, 46, 53, 296, 984, 1243
EarI CTCTTC 6 cut(s) 37, 46, 53, 296, 984, 1243
Ecl136II GAGCTC 1 cut(s) 1253
Eco24I GRGCYC 1 cut(s) 1255
Eco31I GGTCTC 1 cut(s) 134
Eco47I GGWCC 3 cut(s) 326, 350, 485
Eco53kI GAGCTC 1 cut(s) 1253
Eco57I CTGAAG 3 cut(s) 459, 501, 1499
Eco81I CCTNAGG 1 cut(s) 211
Eco88I CYCGRG 2 cut(s) 137, 820
EcoICRI GAGCTC 1 cut(s) 1253
EcoO109I RGGNCCY 2 cut(s) 485, 1448
EcoRII CCWGG 1 cut(s) 1273
EcoT22I ATGCAT 1 cut(s) 805
EcoT38I GRGCYC 1 cut(s) 1255
Esp3I CGTCTC 2 cut(s) 344, 1223
FaeI CATG 4 cut(s) 168, 398, 1049, 1561
FalI AAGNNNNNCTT 4 cut(s) 474, 506, 747, 779
FaqI GGGAC 2 cut(s) 159, 803
FatI CATG 4 cut(s) 164, 394, 1045, 1557
FauI CCCGC 1 cut(s) 1033
FbaI TGATCA 1 cut(s) 1543
FblI GTMKAC 2 cut(s) 235, 1131
Fnu4HI GCNGC 2 cut(s) 345, 602
FokI GGATG 5 cut(s) 325, 1028, 1215, 1313, 1418
FriOI GRGCYC 1 cut(s) 1255
Fsp4HI GCNGC 2 cut(s) 345, 602
FspBI CTAG 1 cut(s) 1452
GlaI GCGC 1 cut(s) 1396
GluI GCNGC 2 cut(s) 345, 602
GsaI CCCAGC 1 cut(s) 383
HaeIII GGCC 1 cut(s) 1450
HapII CCGG 2 cut(s) 1157, 1296
HgaI GACGC 2 cut(s) 56, 1205
HhaI GCGC 1 cut(s) 1397
Hin1II CATG 4 cut(s) 168, 398, 1049, 1561
Hin6I GCGC 1 cut(s) 1395
HinP1I GCGC 1 cut(s) 1395
HinfI GANTC 6 cut(s) 5, 227, 701, 921, 1208, 1380
HpaII CCGG 2 cut(s) 1157, 1296
HphI GGTGA 3 cut(s) 69, 841, 1618
Hpy166II GTNNAC 3 cut(s) 236, 739, 1132
Hpy188I TCNGA 6 cut(s) 107, 281, 448, 654, 1342, 1548
Hpy188III TCNNGA 9 cut(s) 139, 176, 213, 244, 820, 1046, 1240, 1262, 1541
Hpy8I GTNNAC 3 cut(s) 236, 739, 1132
Hpy99I CGWCG 2 cut(s) 227, 263
HpyAV CCTTC 4 cut(s) 476, 905, 1139, 1597
HpyCH4III ACNGT 2 cut(s) 338, 868
HpyCH4IV ACGT 1 cut(s) 1527
HpyF10VI GCNNNNNNNGC 3 cut(s) 842, 973, 1496
HpyF3I CTNAG 7 cut(s) 211, 430, 651, 759, 885, 1088, 1359
HpySE526I ACGT 1 cut(s) 1527
Hsp92II CATG 4 cut(s) 168, 398, 1049, 1561
HspAI GCGC 1 cut(s) 1395
Ksp22I TGATCA 1 cut(s) 1543
Kzo9I GATC 8 cut(s) 107, 271, 775, 795, 1018, 1058, 1468, 1543
LguI GCTCTTC 1 cut(s) 1243
LmnI GCTCC 1 cut(s) 1568
Lsp1109I GCAGC 2 cut(s) 331, 613
LweI GCATC 2 cut(s) 790, 937
MaeI CTAG 1 cut(s) 1452
MaeII ACGT 1 cut(s) 1527
MaeIII GTNAC 2 cut(s) 847, 1008
MalI GATC 8 cut(s) 109, 273, 777, 797, 1020, 1060, 1470, 1545
MbiI CCGCTC 1 cut(s) 512
MboI GATC 8 cut(s) 107, 271, 775, 795, 1018, 1058, 1468, 1543
MfeI CAATTG 1 cut(s) 1491
MflI RGATCY 2 cut(s) 107, 1058
MhlI GDGCHC 2 cut(s) 289, 1255
MluI ACGCGT 1 cut(s) 65
MlyI GAGTC 3 cut(s) 14, 236, 1217
Mph1103I ATGCAT 1 cut(s) 805
MseI TTAA 4 cut(s) 687, 750, 1109, 1523
MslI CAYNNNNRTG 1 cut(s) 1556
MspA1I CMGCKG 1 cut(s) 1519
MspI CCGG 2 cut(s) 1157, 1296
MspR9I CCNGG 2 cut(s) 1157, 1275
MunI CAATTG 1 cut(s) 1491
MvaI CCWGG 1 cut(s) 1275
MvnI CGCG 4 cut(s) 67, 245, 251, 1505
MwoI GCNNNNNNNGC 3 cut(s) 842, 973, 1496
NciI CCSGG 1 cut(s) 1157
NdeII GATC 8 cut(s) 107, 271, 775, 795, 1018, 1058, 1468, 1543
NlaIII CATG 4 cut(s) 168, 398, 1049, 1561
NlaIV GGNNCC 3 cut(s) 328, 519, 1539
NruI TCGCGA 1 cut(s) 245
NsiI ATGCAT 1 cut(s) 805
NspI RCATGY 1 cut(s) 398
PaeR7I CTCGAG 1 cut(s) 137
PagI TCATGA 1 cut(s) 1045
PciI ACATGT 1 cut(s) 394
PciSI GCTCTTC 1 cut(s) 1243
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PfeI GAWTC 3 cut(s) 701, 921, 1380
PflFI GACNNNGTC 1 cut(s) 263
PflMI CCANNNNNTGG 1 cut(s) 1442
PkrI GCNGC 2 cut(s) 346, 603
PleI GAGTC 3 cut(s) 13, 235, 1216
PpsI GAGTC 3 cut(s) 13, 235, 1216
PpuMI RGGWCCY 1 cut(s) 485
PscI ACATGT 1 cut(s) 394
Psp124BI GAGCTC 1 cut(s) 1255
Psp1406I AACGTT 1 cut(s) 1527
Psp5II RGGWCCY 1 cut(s) 485
Psp6I CCWGG 1 cut(s) 1273
PspFI CCCAGC 1 cut(s) 379
PspGI CCWGG 1 cut(s) 1273
PspN4I GGNNCC 3 cut(s) 328, 519, 1539
PspPI GGNCC 4 cut(s) 326, 350, 485, 1448
PspPPI RGGWCCY 1 cut(s) 485
PsuI RGATCY 2 cut(s) 107, 1058
PsyI GACNNNGTC 1 cut(s) 263
RruI TCGCGA 1 cut(s) 245
RsaI GTAC 4 cut(s) 202, 323, 419, 1187
RsaNI GTAC 4 cut(s) 201, 322, 418, 1186
RseI CAYNNNNRTG 1 cut(s) 1556
SacI GAGCTC 1 cut(s) 1255
SapI GCTCTTC 1 cut(s) 1243
SaqAI TTAA 4 cut(s) 687, 750, 1109, 1523
SatI GCNGC 2 cut(s) 345, 602
Sau3AI GATC 8 cut(s) 107, 271, 775, 795, 1018, 1058, 1468, 1543
Sau96I GGNCC 4 cut(s) 326, 350, 485, 1448
SchI GAGTC 3 cut(s) 14, 236, 1217
ScrFI CCNGG 2 cut(s) 1157, 1275
SduI GDGCHC 2 cut(s) 289, 1255
SfaNI GCATC 2 cut(s) 790, 937
Sfr274I CTCGAG 1 cut(s) 137
SinI GGWCC 3 cut(s) 326, 350, 485
SlaI CTCGAG 1 cut(s) 137
SmiMI CAYNNNNRTG 1 cut(s) 1556
SmlI CTYRAG 2 cut(s) 137, 1254
SmoI CTYRAG 2 cut(s) 137, 1254
SsiI CCGC 4 cut(s) 512, 1040, 1505, 1519
SspMI CTAG 1 cut(s) 1452
SstI GAGCTC 1 cut(s) 1255
StyD4I CCNGG 2 cut(s) 1155, 1273
TaaI ACNGT 2 cut(s) 338, 868
TaiI ACGT 1 cut(s) 1530
TaqI TCGA 8 cut(s) 138, 152, 258, 699, 798, 903, 1164, 1383
TatI WGTACW 1 cut(s) 1185
TfiI GAWTC 3 cut(s) 701, 921, 1380
Tru1I TTAA 4 cut(s) 687, 750, 1109, 1523
Tru9I TTAA 4 cut(s) 687, 750, 1109, 1523
TscAI CASTG 5 cut(s) 58, 748, 1060, 1423, 1447
TseI GCWGC 2 cut(s) 344, 601
TspDTI ATGAA 5 cut(s) 138, 329, 1062, 1420, 1616
TspGWI ACGGA 2 cut(s) 339, 633
TspRI CASTG 5 cut(s) 58, 748, 1060, 1423, 1447
Tth111I GACNNNGTC 1 cut(s) 263
Van91I CCANNNNNTGG 1 cut(s) 1442
VpaK11BI GGWCC 3 cut(s) 326, 350, 485
XapI RAATTY 4 cut(s) 89, 449, 1364, 1427
XceI RCATGY 1 cut(s) 398
XhoI CTCGAG 1 cut(s) 137
XmiI GTMKAC 2 cut(s) 235, 1131
XspI CTAG 1 cut(s) 1452
Zsp2I ATGCAT 1 cut(s) 805
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.