Rh3BG191000

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0009299
Physical Location & Seq
Reverse (-)
16906611 .. 16910577
3967 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG191000.1

Sequence Viewer

Length: 1647 bp
ATGCTTAGCTCTCTCCACTCTTTCTCATCATTCACTCTCCTCCCCACTCCTTCTTCTCCAAAACCCACCACTCCATTCCACTCCTTCTCCATCAGAGCCTCAACCAACAAGGACCCCAGTTCAAACTCCAAGCCCACCACAACCTCCAAACCAAGCTCGTGGGTCAGCCCGGATTGGCTCACTTCCCTGACCAGCTCCCTGACCATATCCAAAAACGACGACTCGGGCATTCCCGTCGCCTCCGCCAAGCTCGATGACGTGTCCGATCTTCTGGGTGGTGCTCTGTTCTTGCCTCTGTTCAAGTGGATGAACCAGTACGGCCCAATATACAGGCTGGCGGCTGGGCCCAGAAACTTCGTGGTGGTCAGTGACCCTGCTATCGCTAAACATGTGCTTAGGAATTATGGCAAGTACGCCAAGGGTCTTGTTGCCGAGGTCTCCGAGTTTTTGTTCGGGTCGGGTTTTGCTATTGCTGAAGGCTCACTTTGGACGGCTAGGCGCAGGGCTGTGGTGCCATCTCTTCATAAGAAGTACTTGTCCGTGATAGTTGATCGGGTATTTTGCAAATGTGCTGATAGATTAGTGGAGAAGCTACAAGCTGATGCACTTAGTGGAACTGCTATAAACATGGAGGAAAAGTTTTGTCAGTTAACTCTTGATGTTATAGGGCTGTCTTTATTCAACTACAATTTTGATTCTCTTAATACTGATAGCCCCGTCATTGATGCCGTTTACACTGCATTGAAAGAAGCGGAGCTTCGTTCTACTGATATATTACCATATTGGAAGGTTGATGCTCTGTGTAAAATAATCCCAAGACAAATAAAAGCTGAAAAAGCAGTTTCAATTATCAGGAGTTCTGTTGAAGAACTTATTGCTAAATGCAAAGCAATTGTGGAAGCTGAGGGTGAAAGAATTGATGAGGAAGAATATTTAAATGAAGCTGATCCAAGCATCCTTCGGTTTTTGCTTGCAAGTAGAGAAGAGGTTTCAAGTGTACAATTACGAGATGATCTTTTGTCTATGTTGGTTGCTGGACATGAGACAACTGGTTCAGTATTGACTTGGACGCTATATCTTCTAAGTAAGGATTCCCGTTCATTAGTGAAAGCACAGGAAGAAGTTGACACAGTTTTACAGGGACGACGTCCTTCCTATGATGACATAAAGGATCTGAAGTTCTTAACTCGCTGCATAATGGAATCTTTGCGTCTCTTTCCACATCCTCCTGTTTTGATAAGAAGGGCTCAAGTTGCTGACGTGCTCCCTGGAAATTACAAAGTCAACCCTGGTCAGGATATTATGATATCGGTATATAATATCCATCGCTCTTCAGAGGTGTGGGAGAGAGCAGAAGAATTTTCACCAGAGAGATTTGATATGGAAGGTCCTATACCCAATGAAACGAATACAGATTTCAGGTTCATTCCATTCAGTGGAGGGCCACGAAAGTGTGTTGGTGATCAGTTTGCTTTGCTGGAAGCTATTGTTGCTCTGGCAATCTTTATTCAGCACCTGAACTTTGAGCTTGTTCCTGATCAGAACATTAGCATGACAACAGGAGCAACAATTCACACTACAAATGGTTTGTACATGAAATTGAGTCAACGGCAAGCAAAATCTGCGTTTGCTGCTTCATCCATCTAA

Protein Analysis

548

Amino Acids

60.86

Weight (kDa)

5.95

Isoelectric Point (pI)

38.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 99 - 521 3.4e-79 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1150
AccB1I GGYRCC 1 cut(s) 511
AccB7I CCANNNNNTGG 1 cut(s) 1436
AciI CCGC 3 cut(s) 243, 338, 752
AclWI GGATC 2 cut(s) 941, 1179
AcsI RAATTY 1 cut(s) 1358
AcuI CTGAAG 3 cut(s) 495, 1196, 1317
AcyI GRCGYC 1 cut(s) 1147
AdeI CACNNNGTG 1 cut(s) 611
AfaI GTAC 5 cut(s) 317, 413, 533, 999, 1592
AfiI CCNNNNNNNGG 2 cut(s) 1294, 1436
AflIII ACRYGT 2 cut(s) 258, 388
AgsI TTSAA 7 cut(s) 123, 301, 682, 745, 846, 866, 993
AjiI CACGTC 2 cut(s) 259, 1261
AjnI CCWGG 2 cut(s) 1267, 1288
AleI CACNNNNGTG 1 cut(s) 1450
AloI GAACNNNNNNTCC 2 cut(s) 1163, 1195
Alw21I GWGCWC 2 cut(s) 283, 1266
Alw26I GTCTC 3 cut(s) 442, 1037, 1217
AlwI GGATC 2 cut(s) 941, 1179
AlwNI CAGNNNCTG 1 cut(s) 1516
Ama87I CYCGRG 1 cut(s) 223
AoxI GGCC 3 cut(s) 319, 344, 1442
ApaI GGGCCC 1 cut(s) 348
ApeKI GCWGC 2 cut(s) 1191, 1631
ApoI RAATTY 1 cut(s) 1358
AspLEI GCGC 1 cut(s) 501
AspS9I GGNCC 6 cut(s) 112, 320, 344, 345, 1388, 1442
AsuC2I CCSGG 1 cut(s) 170
AsuHPI GGTGA 3 cut(s) 920, 1356, 1472
AvaI CYCGRG 1 cut(s) 223
AvaII GGWCC 2 cut(s) 112, 1388
BaeGI GKGCMC 1 cut(s) 348
BanI GGYRCC 1 cut(s) 511
BanII GRGCYC 2 cut(s) 348, 1249
BarI GAAGNNNNNNTAC 2 cut(s) 1377, 1409
BauI CACGAG 1 cut(s) 157
Bbv12I GWGCWC 2 cut(s) 283, 1266
BbvCI CCTCAGC 1 cut(s) 903
BbvI GCAGC 2 cut(s) 1178, 1618
BccI CCATC 3 cut(s) 98, 523, 1332
BceAI ACGGC 4 cut(s) 334, 507, 713, 1625
BcgI CGANNNNNNTGC 2 cut(s) 217, 251
BciT130I CCWGG 2 cut(s) 1269, 1290
BclI TGATCA 2 cut(s) 1462, 1537
BcnI CCSGG 1 cut(s) 170
BcoDI GTCTC 3 cut(s) 442, 1037, 1217
BfaI CTAG 1 cut(s) 495
BisI GCNGC 3 cut(s) 339, 1192, 1632
BlpI GCTNAGC 1 cut(s) 5
BlsI GCNGC 3 cut(s) 340, 1193, 1633
BmcAI AGTACT 1 cut(s) 533
Bme1390I CCNGG 3 cut(s) 170, 1269, 1290
Bme18I GGWCC 2 cut(s) 112, 1388
BmeT110I CYCGRG 1 cut(s) 223
BmgBI CACGTC 2 cut(s) 259, 1261
BmgT120I GGNCC 6 cut(s) 112, 320, 344, 345, 1388, 1442
BmiI GGNNCC 3 cut(s) 114, 346, 513
BmrFI CCNGG 3 cut(s) 170, 1269, 1290
BmrI ACTGGG 1 cut(s) 111
BmsI GCATC 4 cut(s) 592, 715, 784, 963
BmuI ACTGGG 1 cut(s) 111
Bpu10I CCTNAGC 2 cut(s) 395, 903
Bpu1102I GCTNAGC 1 cut(s) 5
BpuEI CTTGAG 1 cut(s) 1233
BpuMI CCSGG 1 cut(s) 170
BsaHI GRCGYC 1 cut(s) 1147
BsaI GGTCTC 1 cut(s) 442
BsaJI CCNNGG 4 cut(s) 417, 432, 1267, 1288
BsaXI ACNNNNNCTCC 4 cut(s) 71, 101, 623, 653
Bsc4I CCNNNNNNNGG 2 cut(s) 1294, 1436
Bse1I ACTGG 3 cut(s) 117, 313, 1054
BseBI CCWGG 2 cut(s) 1269, 1290
BseDI CCNNGG 4 cut(s) 417, 432, 1267, 1288
BseGI GGATG 4 cut(s) 312, 954, 1222, 1637
BseLI CCNNNNNNNGG 2 cut(s) 1294, 1436
BseMII CTCAG 1 cut(s) 894
BseNI ACTGG 3 cut(s) 117, 313, 1054
BseRI GAGGAG 1 cut(s) 29
BseSI GKGCMC 1 cut(s) 348
BseXI GCAGC 2 cut(s) 1178, 1618
BseYI CCCAGC 1 cut(s) 341
BshFI GGCC 3 cut(s) 321, 346, 1444
BshNI GGYRCC 1 cut(s) 511
BsiHKAI GWGCWC 2 cut(s) 283, 1266
BsiHKCI CYCGRG 1 cut(s) 223
BsiSI CCGG 1 cut(s) 170
BslFI GGGAC 1 cut(s) 1155
BslI CCNNNNNNNGG 2 cut(s) 1294, 1436
BsmAI GTCTC 3 cut(s) 442, 1037, 1217
BsmBI CGTCTC 1 cut(s) 1217
BsmFI GGGAC 1 cut(s) 1155
BsmI GAATGC 1 cut(s) 228
BsnI GGCC 3 cut(s) 321, 346, 1444
Bso31I GGTCTC 1 cut(s) 442
BsoBI CYCGRG 1 cut(s) 223
Bsp120I GGGCCC 1 cut(s) 344
Bsp1286I GDGCHC 4 cut(s) 283, 348, 1249, 1266
Bsp1407I TGTACA 2 cut(s) 997, 1590
Bsp143I GATC 7 cut(s) 265, 550, 946, 1012, 1171, 1462, 1537
Bsp1720I GCTNAGC 1 cut(s) 5
BspACI CCGC 3 cut(s) 243, 338, 752
BspANI GGCC 3 cut(s) 321, 346, 1444
BspCNI CTCAG 1 cut(s) 895
BspLI GGNNCC 3 cut(s) 114, 346, 513
BspPI GGATC 2 cut(s) 941, 1179
BspQI GCTCTTC 1 cut(s) 1336
BspT107I GGYRCC 1 cut(s) 511
BspTNI GGTCTC 1 cut(s) 442
BsrGI TGTACA 2 cut(s) 997, 1590
BsrI ACTGG 3 cut(s) 117, 313, 1054
BssECI CCNNGG 4 cut(s) 417, 432, 1267, 1288
BssMI GATC 7 cut(s) 265, 550, 946, 1012, 1171, 1462, 1537
BssNI GRCGYC 1 cut(s) 1147
BssSI CACGAG 1 cut(s) 157
BssT1I CCWWGG 1 cut(s) 417
Bst2BI CACGAG 1 cut(s) 157
Bst2UI CCWGG 2 cut(s) 1269, 1290
Bst4CI ACNGT 1 cut(s) 1132
Bst6I CTCTTC 3 cut(s) 525, 978, 1336
BstACI GRCGYC 1 cut(s) 1147
BstAPI GCANNNNNTGC 1 cut(s) 1622
BstAUI TGTACA 2 cut(s) 997, 1590
BstC8I GCNNGC 3 cut(s) 336, 972, 1614
BstDEI CTNAG 5 cut(s) 5, 395, 608, 903, 1082
BstF5I GGATG 4 cut(s) 312, 954, 1222, 1637
BstHHI GCGC 1 cut(s) 501
BstKTI GATC 7 cut(s) 268, 553, 949, 1015, 1174, 1465, 1540
BstMAI GTCTC 3 cut(s) 442, 1037, 1217
BstMBI GATC 7 cut(s) 265, 550, 946, 1012, 1171, 1462, 1537
BstMWI GCNNNNNNNGC 5 cut(s) 836, 1253, 1490, 1622, 1631
BstNI CCWGG 2 cut(s) 1269, 1290
BstNSI RCATGY 1 cut(s) 392
BstSCI CCNGG 3 cut(s) 168, 1267, 1288
BstSLI GKGCMC 1 cut(s) 348
BstV1I GCAGC 2 cut(s) 1178, 1618
BstX2I RGATCY 1 cut(s) 1171
BstXI CCANNNNNNTGG 1 cut(s) 159
BstYI RGATCY 1 cut(s) 1171
BsuRI GGCC 3 cut(s) 321, 346, 1444
BtgZI GCGATG 1 cut(s) 1310
BtrI CACGTC 2 cut(s) 259, 1261
BtsCI GGATG 4 cut(s) 312, 954, 1222, 1637
BtsI GCAGTG 1 cut(s) 735
BtsIMutI CAGTG 3 cut(s) 373, 735, 1441
Cac8I GCNNGC 3 cut(s) 336, 972, 1614
CaiI CAGNNNCTG 1 cut(s) 1516
CfoI GCGC 1 cut(s) 501
Cfr13I GGNCC 6 cut(s) 112, 320, 344, 345, 1388, 1442
CseI GACGC 2 cut(s) 1078, 1199
Csp6I GTAC 5 cut(s) 316, 412, 532, 998, 1591
CviAII CATG 5 cut(s) 389, 628, 1040, 1552, 1594
CviQI GTAC 5 cut(s) 316, 412, 532, 998, 1591
DdeI CTNAG 5 cut(s) 5, 395, 608, 903, 1082
DpnI GATC 7 cut(s) 267, 552, 948, 1014, 1173, 1464, 1539
DpnII GATC 7 cut(s) 265, 550, 946, 1012, 1171, 1462, 1537
DraI TTTAAA 1 cut(s) 936
DraIII CACNNNGTG 1 cut(s) 611
Eam1104I CTCTTC 3 cut(s) 525, 978, 1336
EarI CTCTTC 3 cut(s) 525, 978, 1336
EciI GGCGGA 1 cut(s) 232
Eco130I CCWWGG 1 cut(s) 417
Eco24I GRGCYC 2 cut(s) 348, 1249
Eco31I GGTCTC 1 cut(s) 442
Eco32I GATATC 1 cut(s) 1308
Eco47I GGWCC 2 cut(s) 112, 1388
Eco57I CTGAAG 3 cut(s) 495, 1196, 1317
Eco88I CYCGRG 1 cut(s) 223
EcoO109I RGGNCCY 2 cut(s) 112, 1388
EcoRII CCWGG 2 cut(s) 1267, 1288
EcoRV GATATC 1 cut(s) 1308
EcoT14I CCWWGG 1 cut(s) 417
EcoT38I GRGCYC 2 cut(s) 348, 1249
ErhI CCWWGG 1 cut(s) 417
Esp3I CGTCTC 1 cut(s) 1217
FaeI CATG 5 cut(s) 392, 631, 1043, 1555, 1597
FalI AAGNNNNNCTT 6 cut(s) 468, 500, 518, 550, 741, 773
FaqI GGGAC 1 cut(s) 1155
FatI CATG 5 cut(s) 388, 627, 1039, 1551, 1593
FbaI TGATCA 2 cut(s) 1462, 1537
Fnu4HI GCNGC 3 cut(s) 339, 1192, 1632
FokI GGATG 4 cut(s) 319, 941, 1209, 1624
FriOI GRGCYC 2 cut(s) 348, 1249
Fsp4HI GCNGC 3 cut(s) 339, 1192, 1632
FspBI CTAG 1 cut(s) 495
GlaI GCGC 1 cut(s) 500
GluI GCNGC 3 cut(s) 339, 1192, 1632
GsaI CCCAGC 1 cut(s) 345
HaeIII GGCC 3 cut(s) 321, 346, 1444
HapII CCGG 1 cut(s) 170
HgaI GACGC 2 cut(s) 1078, 1199
HhaI GCGC 1 cut(s) 501
Hin1I GRCGYC 1 cut(s) 1147
Hin1II CATG 5 cut(s) 392, 631, 1043, 1555, 1597
Hin6I GCGC 1 cut(s) 499
HinP1I GCGC 1 cut(s) 499
HincII GTYRAC 4 cut(s) 651, 1126, 1285, 1607
HindII GTYRAC 4 cut(s) 651, 1126, 1285, 1607
HinfI GANTC 5 cut(s) 221, 695, 1091, 1202, 1603
HpaI GTTAAC 1 cut(s) 651
HpaII CCGG 1 cut(s) 170
HphI GGTGA 3 cut(s) 920, 1356, 1472
Hpy166II GTNNAC 6 cut(s) 651, 733, 998, 1126, 1285, 1607
Hpy188I TCNGA 6 cut(s) 95, 265, 442, 1176, 1336, 1542
Hpy188III TCNNGA 4 cut(s) 656, 853, 1295, 1535
Hpy8I GTNNAC 6 cut(s) 651, 733, 998, 1126, 1285, 1607
Hpy99I CGWCG 3 cut(s) 221, 239, 1149
HpyAV CCTTC 8 cut(s) 60, 94, 470, 781, 968, 1161, 1236, 1379
HpyCH4III ACNGT 1 cut(s) 1132
HpyCH4IV ACGT 3 cut(s) 258, 1147, 1260
HpyCH4V TGCA 6 cut(s) 564, 605, 740, 885, 974, 1194
HpyF10VI GCNNNNNNNGC 5 cut(s) 836, 1253, 1490, 1622, 1631
HpyF3I CTNAG 5 cut(s) 5, 395, 608, 903, 1082
HpySE526I ACGT 3 cut(s) 258, 1147, 1260
Hsp92I GRCGYC 1 cut(s) 1147
Hsp92II CATG 5 cut(s) 392, 631, 1043, 1555, 1597
HspAI GCGC 1 cut(s) 499
Ksp22I TGATCA 2 cut(s) 1462, 1537
KspAI GTTAAC 1 cut(s) 651
Kzo9I GATC 7 cut(s) 265, 550, 946, 1012, 1171, 1462, 1537
LguI GCTCTTC 1 cut(s) 1336
LmnI GCTCC 4 cut(s) 200, 754, 1269, 1562
Lsp1109I GCAGC 2 cut(s) 1178, 1618
LweI GCATC 4 cut(s) 592, 715, 784, 963
MaeI CTAG 1 cut(s) 495
MaeII ACGT 3 cut(s) 258, 1147, 1260
MaeIII GTNAC 1 cut(s) 368
MalI GATC 7 cut(s) 267, 552, 948, 1014, 1173, 1464, 1539
MboI GATC 7 cut(s) 265, 550, 946, 1012, 1171, 1462, 1537
MfeI CAATTG 1 cut(s) 891
MflI RGATCY 1 cut(s) 1171
MhlI GDGCHC 4 cut(s) 283, 348, 1249, 1266
MlyI GAGTC 2 cut(s) 215, 1612
MseI TTAA 4 cut(s) 650, 702, 935, 1184
MslI CAYNNNNRTG 2 cut(s) 1450, 1550
MspI CCGG 1 cut(s) 170
MspR9I CCNGG 3 cut(s) 170, 1269, 1290
MunI CAATTG 1 cut(s) 891
Mva1269I GAATGC 1 cut(s) 228
MvaI CCWGG 2 cut(s) 1269, 1290
MwoI GCNNNNNNNGC 5 cut(s) 836, 1253, 1490, 1622, 1631
NciI CCSGG 1 cut(s) 170
NdeII GATC 7 cut(s) 265, 550, 946, 1012, 1171, 1462, 1537
NlaIII CATG 5 cut(s) 392, 631, 1043, 1555, 1597
NlaIV GGNNCC 3 cut(s) 114, 346, 513
NmeAIII GCCGAG 1 cut(s) 457
NmuCI GTSAC 1 cut(s) 368
NspI RCATGY 1 cut(s) 392
OliI CACNNNNGTG 1 cut(s) 1450
PciI ACATGT 1 cut(s) 388
PciSI GCTCTTC 1 cut(s) 1336
PctI GAATGC 1 cut(s) 228
PfeI GAWTC 3 cut(s) 695, 1091, 1202
PflFI GACNNNGTC 1 cut(s) 1146
PflMI CCANNNNNTGG 1 cut(s) 1436
PkrI GCNGC 3 cut(s) 340, 1193, 1633
PleI GAGTC 2 cut(s) 215, 1611
PpsI GAGTC 2 cut(s) 215, 1611
PpuMI RGGWCCY 2 cut(s) 112, 1388
PscI ACATGT 1 cut(s) 388
Psp5II RGGWCCY 2 cut(s) 112, 1388
Psp6I CCWGG 2 cut(s) 1267, 1288
PspFI CCCAGC 1 cut(s) 341
PspGI CCWGG 2 cut(s) 1267, 1288
PspN4I GGNNCC 3 cut(s) 114, 346, 513
PspOMI GGGCCC 1 cut(s) 344
PspPI GGNCC 6 cut(s) 112, 320, 344, 345, 1388, 1442
PspPPI RGGWCCY 2 cut(s) 112, 1388
PstNI CAGNNNCTG 1 cut(s) 1516
PsuI RGATCY 1 cut(s) 1171
PsyI GACNNNGTC 1 cut(s) 1146
RsaI GTAC 5 cut(s) 317, 413, 533, 999, 1592
RsaNI GTAC 5 cut(s) 316, 412, 532, 998, 1591
RseI CAYNNNNRTG 2 cut(s) 1450, 1550
SapI GCTCTTC 1 cut(s) 1336
SaqAI TTAA 4 cut(s) 650, 702, 935, 1184
SatI GCNGC 3 cut(s) 339, 1192, 1632
Sau3AI GATC 7 cut(s) 265, 550, 946, 1012, 1171, 1462, 1537
Sau96I GGNCC 6 cut(s) 112, 320, 344, 345, 1388, 1442
ScaI AGTACT 1 cut(s) 533
SchI GAGTC 2 cut(s) 215, 1612
ScrFI CCNGG 3 cut(s) 170, 1269, 1290
SduI GDGCHC 4 cut(s) 283, 348, 1249, 1266
SfaNI GCATC 4 cut(s) 592, 715, 784, 963
SinI GGWCC 2 cut(s) 112, 1388
SmiI ATTTAAAT 1 cut(s) 936
SmiMI CAYNNNNRTG 2 cut(s) 1450, 1550
SmlI CTYRAG 1 cut(s) 1248
SmoI CTYRAG 1 cut(s) 1248
SsiI CCGC 3 cut(s) 243, 338, 752
SspI AATATT 1 cut(s) 932
SspMI CTAG 1 cut(s) 495
StyD4I CCNGG 3 cut(s) 168, 1267, 1288
StyI CCWWGG 1 cut(s) 417
SwaI ATTTAAAT 1 cut(s) 936
TaaI ACNGT 1 cut(s) 1132
TaiI ACGT 3 cut(s) 261, 1150, 1263
TaqI TCGA 1 cut(s) 252
TatI WGTACW 3 cut(s) 531, 997, 1590
TauI GCSGC 1 cut(s) 341
TfiI GAWTC 3 cut(s) 695, 1091, 1202
Tru1I TTAA 4 cut(s) 650, 702, 935, 1184
Tru9I TTAA 4 cut(s) 650, 702, 935, 1184
TscAI CASTG 3 cut(s) 373, 742, 1441
TseFI GTSAC 1 cut(s) 368
TseI GCWGC 2 cut(s) 1191, 1631
Tsp45I GTSAC 1 cut(s) 368
TspDTI ATGAA 8 cut(s) 323, 512, 954, 1089, 1414, 1416, 1610, 1626
TspGWI ACGGA 1 cut(s) 529
TspRI CASTG 3 cut(s) 373, 742, 1441
Tth111I GACNNNGTC 1 cut(s) 1146
Van91I CCANNNNNTGG 1 cut(s) 1436
VpaK11BI GGWCC 2 cut(s) 112, 1388
XapI RAATTY 1 cut(s) 1358
XceI RCATGY 1 cut(s) 392
XcmI CCANNNNNNNNNTGG 1 cut(s) 355
XspI CTAG 1 cut(s) 495
ZraI GACGTC 1 cut(s) 1148
ZrmI AGTACT 1 cut(s) 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.