Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003539

Physical Location & Seq

Genomic Coordinates Reverse (-)

20175370 .. 20179070

Gene Structure 3701 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G54500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1947 bp

ATGTTTGATTGGGAAGAAGAAGAGCTTACTAATATGATATGGGGTGATGATGCTGAGACAGGCGACCATATTGTGCCTTTTAAAGTCAGAAGTGAACAACTTAACAAAAAGGAACAGATTGAGGAATCTAAGACAGCTGAGCAAAAGATAACTGGGACTAAAATTGACCTCCATGATAAAAATTTGGGGAGCAGTTCGAGCCATAATGTTGATGAGGGGCTTCCTCAGCCAGATTTCTGTATGAGCTCATGGCCTGACACGTCGCTAACTAATGCTACAAAGGTTGATCAAGATTTGAGTGCGACTGAACTTTCAAAATGCTTAGCTGAGCCAGTCAGATATGATTCAACAAGAGAGAAAACGTCTGAACTTGGGAAAGGCCCTGATATTTTTCATAGCTCTGATGAGAGTAAAGAGCAAGGTGATTTTGATGACTACAGCTGGGCCAACATTGGTAGTTTTGATGATCTTGATCGAATGTTCAGCAATGATGTCCCTATATTTGGTGATGGCAGTCTCAGCGGTGGTGATGAGTTATGGTCATCTTCTAAAGATGTATCCAATAGCCCAAAATCATTATCATCAATGTTGGACTCTCAAGATCTAGGATTGGATATTAGAACTGAGTTTGAGCAACAAGAGAACCAGCAATTTCCATTGACTGGAAAAGCCAATGGTCTGTCATCCCAAAGTGTCCCAAGTGTACGTGTAACTCTTAAGGCTGACCAATATCGTGAGCATAAGGGTCAACCCTCAGTTGAGGACCAGCCATATCAACAAAATAAAATGATGAAGTTTTCGAAAATGCCTGGGACTTCTGAGGCAAGGCCCTTTCAAGAGCTGTACGGTCAGAGGATTCCATTTAGTAATTCAGCTGGAAAATGTGTAAATCAGTTGGCACCGCCACAATCGTCTCTGATGGCTGTTAATCTCCTGAGTGAGTCTGAGGGGTCTGGGACATCACACTATTCACATATGCCAAACCAATACATGGCTAATTCTGCTTTTGGTAATCTTGCAAATCCATATTCTAGTGTGCCTGTAATTTCGGCCGTCCAACATCCTGATGTTAGGAATCAGTTGATGCATCCTTCCTACAATCCTGCTACTGCTACCTCAGTAAACATGGCAACAGATGCCTCTGCACGGCCTTCAACAATGACACCACAGGAAAAACTAGAAAAACTTAGACGCAGACAGCAAATGCAGGCAATGCTTGCCATTCAGAGACAACAGCAGCAATTTTCTCATCAGGTGCCTGTAGCAGATCAATCCATTACTCAAAACTGTCTCCAAGATATTCCACTCCAGCTTGTCGACAAAACTAATCTCCAAGGGCTAACTGCAATGCCTTCCTTTGATCCTAGTTCATCTTTGGAACAAGATGATTCTGGCAAATTTGCTGCTGCTGTTGATAATTCAGCAGAATTCGCAGTTCTTTATCGGCTTCAGGATGTTGTAGCAAAGTTGGATATGGGAACACGGACTTGTATACGGGATAGCTTATTCCGGTTGGCTGGTAGTGCAGCTCAGAGACATTACACTAGTGATACATCCCACAGTAACAAGACTAGCCAGGATGACCAGGAAGTCATTCCCAGAGAAGAATCCAGATATAGATATGCTGGGATGCCAGATACAGAAGCAGTGACCAACCCCACAGACAGAACTGTGGCTCATTTGCTCTTTCATAGGCCTTTTGATATGTTGGCGGCAAAGCGAATGGAAGGACCAGAATCACCCGCTTCTTCGAAGATGGGAACTGAAGAAAAAGGGAATTTTCCTAAATGCAGCATACGAGAGACTCACTTAACTAAGCAGAAAGCTCAAAAGGAAGAAGGACCTGCAGATTCACTTGCTTTGGGGAATGCACCCAACTCTGGATCTAGTAGTACTGTTGGTGAGAGGGTTGTTGAAGCATCCCAAGGAAACAAAAGAAAATTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

648

Amino Acids

71.66

Weight (kDa)

5.04

Isoelectric Point (pI)

57.05

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003539)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G54500 AT3G54500 AT3G54500 AT3G54500 AT3G54500 AT3G54500 AT3G54500 AT3G54500
fragaria_vesca	FvH4_7g02700 FvH4_7g02700 FvH4_7g02700 FvH4_7g02700 FvH4_7g02700 FvH4_7g02700 FvH4_7g02700 FvH4_7g02700
malus_domestica	MD02G1290900.v1.1 MD07G1036600.v1.1
prunus_persica	Prupe.2G032000_v2.0.a1 Prupe.2G032000_v2.0.a1 Prupe.2G032000_v2.0.a1 Prupe.2G032000_v2.0.a1
pyrus_communis	pycom02g24590 pycom07g02520 pycom11g13590
rosa_chinensis	RchiOBHm_Chr1g0321761
rosa_laevigata	RLG00000030406
rosa_multiflora	Rmu_sc0003410.1_g000034 Rmu_sc0003909.1_g000003
rosa_rugosa	Rorug01G0034100
rosa_samantha	Rh1AG046700 Rh1BG045200 Rh1CG050800 Rh1DG055100
rosa_wichuraiana	Rw1G004190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1589
Acc36I	ACCTGC	1 cut(s)	1852
AccB1I	GGYRCC	2 cut(s)	898, 1255
AccB7I	CCANNNNNTGG	2 cut(s)	662, 991
AccI	GTMKAC	2 cut(s)	1317, 1492
AciI	CCGC	4 cut(s)	522, 902, 1712, 1743
AclWI	GGATC	2 cut(s)	1355, 1891
AcoI	YGGCCR	1 cut(s)	1050
AcsI	RAATTY	4 cut(s)	181, 1397, 1427, 1777
AcuI	CTGAAG	2 cut(s)	1433, 1785
AfaI	GTAC	3 cut(s)	705, 845, 1894
AfiI	CCNNNNNNNGG	5 cut(s)	503, 662, 991, 1146, 1880
AflII	CTTAAG	1 cut(s)	716
AflIII	ACRYGT	2 cut(s)	258, 706
AgsI	TTSAA	5 cut(s)	315, 348, 836, 1155, 1916
AhlI	ACTAGT	1 cut(s)	1544
AjiI	CACGTC	1 cut(s)	261
AjnI	CCWGG	3 cut(s)	808, 1575, 1584
Alw21I	GWGCWC	1 cut(s)	248
Alw26I	GTCTC	7 cut(s)	50, 521, 918, 1222, 1295, 1528, 1796
AlwI	GGATC	2 cut(s)	1355, 1891
AoxI	GGCC	7 cut(s)	251, 379, 444, 827, 1050, 1148, 1694
ApeKI	GCWGC	5 cut(s)	1237, 1403, 1406, 1526, 1791
ApoI	RAATTY	4 cut(s)	181, 1397, 1427, 1777
Asp700I	GAANNNNTTC	1 cut(s)	1593
AspS9I	GGNCC	6 cut(s)	380, 444, 763, 828, 1730, 1841
AsuHPI	GGTGA	6 cut(s)	56, 434, 518, 539, 1731, 1913
AsuII	TTCGAA	2 cut(s)	800, 1751
AvaII	GGWCC	3 cut(s)	763, 1730, 1841
BanI	GGYRCC	2 cut(s)	898, 1255
BanII	GRGCYC	1 cut(s)	248
Bbv12I	GWGCWC	1 cut(s)	248
BbvCI	CCTCAGC	1 cut(s)	225
BbvI	GCAGC	5 cut(s)	1249, 1390, 1393, 1538, 1803
BccI	CCATC	3 cut(s)	503, 913, 1750
BceAI	ACGGC	2 cut(s)	1037, 1163
BciT130I	CCWGG	3 cut(s)	810, 1577, 1586
BciVI	GTATCC	1 cut(s)	568
BclI	TGATCA	1 cut(s)	286
BcoDI	GTCTC	7 cut(s)	50, 521, 918, 1222, 1295, 1528, 1796
BcuI	ACTAGT	1 cut(s)	1544
BfaI	CTAG	7 cut(s)	605, 1032, 1178, 1365, 1545, 1572, 1887
BfmI	CTRYAG	3 cut(s)	436, 1260, 1845
BfrI	CTTAAG	1 cut(s)	716
BfuAI	ACCTGC	1 cut(s)	1852
BfuI	GTATCC	1 cut(s)	568
BglII	AGATCT	1 cut(s)	601
BisI	GCNGC	6 cut(s)	1238, 1404, 1407, 1527, 1713, 1792
BlpI	GCTNAGC	3 cut(s)	138, 322, 327
BlsI	GCNGC	6 cut(s)	1239, 1405, 1408, 1528, 1714, 1793
BmcAI	AGTACT	1 cut(s)	1894
Bme1390I	CCNGG	3 cut(s)	810, 1577, 1586
Bme18I	GGWCC	3 cut(s)	763, 1730, 1841
BmgBI	CACGTC	1 cut(s)	261
BmgT120I	GGNCC	6 cut(s)	380, 444, 763, 828, 1730, 1841
BmiI	GGNNCC	2 cut(s)	900, 1257
BmrFI	CCNGG	3 cut(s)	810, 1577, 1586
BmrI	ACTGGG	1 cut(s)	162
BmsI	GCATC	6 cut(s)	40, 1074, 1096, 1126, 1620, 1928
BmuI	ACTGGG	1 cut(s)	162
BpmI	CTGGAG	1 cut(s)	1292
Bpu10I	CCTNAGC	1 cut(s)	225
Bpu1102I	GCTNAGC	3 cut(s)	138, 322, 327
Bpu14I	TTCGAA	2 cut(s)	800, 1751
BpuEI	CTTGAG	1 cut(s)	582
BsaAI	YACGTR	1 cut(s)	707
BsaBI	GATNNNNATC	1 cut(s)	471
BsaJI	CCNNGG	3 cut(s)	809, 1333, 1924
BsaWI	WCCGGW	1 cut(s)	1509
Bsc4I	CCNNNNNNNGG	5 cut(s)	503, 662, 991, 1146, 1880
Bse1I	ACTGG	3 cut(s)	157, 332, 667
Bse3DI	GCAATG	3 cut(s)	493, 1218, 1353
Bse8I	GATNNNNATC	1 cut(s)	471
BseBI	CCWGG	3 cut(s)	810, 1577, 1586
BseDI	CCNNGG	3 cut(s)	809, 1333, 1924
BseGI	GGATG	8 cut(s)	683, 1060, 1087, 1459, 1553, 1585, 1635, 1919
BseJI	GATNNNNATC	1 cut(s)	471
BseLI	CCNNNNNNNGG	5 cut(s)	503, 662, 991, 1146, 1880
BseMI	GCAATG	3 cut(s)	493, 1218, 1353
BseNI	ACTGG	3 cut(s)	157, 332, 667
BseX3I	CGGCCG	1 cut(s)	1050
BseXI	GCAGC	5 cut(s)	1249, 1390, 1393, 1538, 1803
BseYI	CCCAGC	2 cut(s)	441, 1625
BsgI	GTGCAG	2 cut(s)	1128, 1545
Bsh1285I	CGRYCG	1 cut(s)	1053
BshFI	GGCC	7 cut(s)	253, 381, 446, 829, 1052, 1150, 1696
BshNI	GGYRCC	2 cut(s)	898, 1255
BsiEI	CGRYCG	1 cut(s)	1053
BsiHKAI	GWGCWC	1 cut(s)	248
BsiSI	CCGG	1 cut(s)	1510
BslFI	GGGAC	5 cut(s)	169, 479, 680, 826, 970
BslI	CCNNNNNNNGG	5 cut(s)	503, 662, 991, 1146, 1880
BsmAI	GTCTC	7 cut(s)	50, 521, 918, 1222, 1295, 1528, 1796
BsmBI	CGTCTC	1 cut(s)	918
BsmFI	GGGAC	5 cut(s)	169, 479, 680, 826, 970
BsmI	GAATGC	1 cut(s)	1873
BsnI	GGCC	7 cut(s)	253, 381, 446, 829, 1052, 1150, 1696
Bsp119I	TTCGAA	2 cut(s)	800, 1751
Bsp1286I	GDGCHC	1 cut(s)	248
Bsp143I	GATC	7 cut(s)	286, 466, 472, 601, 1267, 1360, 1883
Bsp1720I	GCTNAGC	3 cut(s)	138, 322, 327
BspACI	CCGC	4 cut(s)	522, 902, 1712, 1743
BspANI	GGCC	7 cut(s)	253, 381, 446, 829, 1052, 1150, 1696
BspLI	GGNNCC	2 cut(s)	900, 1257
BspMAI	CTGCAG	1 cut(s)	1849
BspMI	ACCTGC	1 cut(s)	1852
BspPI	GGATC	2 cut(s)	1355, 1891
BspQI	GCTCTTC	1 cut(s)	15
BspT104I	TTCGAA	2 cut(s)	800, 1751
BspT107I	GGYRCC	2 cut(s)	898, 1255
BspTI	CTTAAG	1 cut(s)	716
BsrDI	GCAATG	3 cut(s)	493, 1218, 1353
BsrI	ACTGG	3 cut(s)	157, 332, 667
BssECI	CCNNGG	3 cut(s)	809, 1333, 1924
BssMI	GATC	7 cut(s)	286, 466, 472, 601, 1267, 1360, 1883
BssNAI	GTATAC	1 cut(s)	1493
BssT1I	CCWWGG	2 cut(s)	1333, 1924
Bst1107I	GTATAC	1 cut(s)	1493
Bst2UI	CCWGG	3 cut(s)	810, 1577, 1586
Bst4CI	ACNGT	5 cut(s)	848, 1289, 1562, 1672, 1897
Bst6I	CTCTTC	1 cut(s)	15
BstAFI	CTTAAG	1 cut(s)	716
BstAPI	GCANNNNNTGC	3 cut(s)	1136, 1213, 1217
BstBAI	YACGTR	1 cut(s)	707
BstBI	TTCGAA	2 cut(s)	800, 1751
BstC8I	GCNNGC	2 cut(s)	1209, 1218
BstF5I	GGATG	8 cut(s)	683, 1060, 1087, 1459, 1553, 1585, 1635, 1919
BstKTI	GATC	7 cut(s)	289, 469, 475, 604, 1270, 1363, 1886
BstMAI	GTCTC	7 cut(s)	50, 521, 918, 1222, 1295, 1528, 1796
BstMBI	GATC	7 cut(s)	286, 466, 472, 601, 1267, 1360, 1883
BstMCI	CGRYCG	1 cut(s)	1053
BstMWI	GCNNNNNNNGC	9 cut(s)	198, 226, 519, 1001, 1136, 1213, 1217, 1430, 1523
BstNI	CCWGG	3 cut(s)	810, 1577, 1586
BstSCI	CCNGG	3 cut(s)	808, 1575, 1584
BstSFI	CTRYAG	3 cut(s)	436, 1260, 1845
BstV1I	GCAGC	5 cut(s)	1249, 1390, 1393, 1538, 1803
BstX2I	RGATCY	2 cut(s)	601, 1883
BstYI	RGATCY	2 cut(s)	601, 1883
BstZ17I	GTATAC	1 cut(s)	1493
BstZI	CGGCCG	1 cut(s)	1050
BsuI	GTATCC	1 cut(s)	568
BsuRI	GGCC	7 cut(s)	253, 381, 446, 829, 1052, 1150, 1696
BtrI	CACGTC	1 cut(s)	261
BtsCI	GGATG	8 cut(s)	683, 1060, 1087, 1459, 1553, 1585, 1635, 1919
BtsI	GCAGTG	1 cut(s)	1653
BtsIMutI	CAGTG	1 cut(s)	1653
BveI	ACCTGC	1 cut(s)	1852
Cac8I	GCNNGC	2 cut(s)	1209, 1218
Cfr13I	GGNCC	6 cut(s)	380, 444, 763, 828, 1730, 1841
CseI	GACGC	1 cut(s)	1200
Csp6I	GTAC	3 cut(s)	704, 844, 1893
CviAII	CATG	4 cut(s)	173, 249, 991, 1126
CviQI	GTAC	3 cut(s)	704, 844, 1893
DpnI	GATC	7 cut(s)	288, 468, 474, 603, 1269, 1362, 1885
DpnII	GATC	7 cut(s)	286, 466, 472, 601, 1267, 1360, 1883
DraI	TTTAAA	1 cut(s)	82
DrdI	GACNNNNNNGTC	1 cut(s)	1589
DseDI	GACNNNNNNGTC	1 cut(s)	1589
EaeI	YGGCCR	1 cut(s)	1050
EagI	CGGCCG	1 cut(s)	1050
Eam1104I	CTCTTC	1 cut(s)	15
EarI	CTCTTC	1 cut(s)	15
Ecl136II	GAGCTC	1 cut(s)	246
EclXI	CGGCCG	1 cut(s)	1050
Eco130I	CCWWGG	2 cut(s)	1333, 1924
Eco147I	AGGCCT	1 cut(s)	1696
Eco24I	GRGCYC	1 cut(s)	248
Eco47I	GGWCC	3 cut(s)	763, 1730, 1841
Eco52I	CGGCCG	1 cut(s)	1050
Eco53kI	GAGCTC	1 cut(s)	246
Eco57I	CTGAAG	2 cut(s)	1433, 1785
EcoICRI	GAGCTC	1 cut(s)	246
EcoO109I	RGGNCCY	3 cut(s)	380, 828, 1841
EcoRI	GAATTC	1 cut(s)	1427
EcoRII	CCWGG	3 cut(s)	808, 1575, 1584
EcoT14I	CCWWGG	2 cut(s)	1333, 1924
EcoT22I	ATGCAT	1 cut(s)	1089
EcoT38I	GRGCYC	1 cut(s)	248
ErhI	CCWWGG	2 cut(s)	1333, 1924
Esp3I	CGTCTC	1 cut(s)	918
FaeI	CATG	4 cut(s)	176, 252, 994, 1129
FalI	AAGNNNNNCTT	2 cut(s)	9, 41
FaqI	GGGAC	5 cut(s)	169, 479, 680, 826, 970
FatI	CATG	4 cut(s)	172, 248, 990, 1125
FauI	CCCGC	1 cut(s)	1750
FauNDI	CATATG	1 cut(s)	975
FbaI	TGATCA	1 cut(s)	286
FblI	GTMKAC	2 cut(s)	1317, 1492
Fnu4HI	GCNGC	6 cut(s)	1238, 1404, 1407, 1527, 1713, 1792
FokI	GGATG	8 cut(s)	670, 1047, 1074, 1466, 1540, 1592, 1642, 1906
FriOI	GRGCYC	1 cut(s)	248
Fsp4HI	GCNGC	6 cut(s)	1238, 1404, 1407, 1527, 1713, 1792
FspBI	CTAG	7 cut(s)	605, 1032, 1178, 1365, 1545, 1572, 1887
GluI	GCNGC	6 cut(s)	1238, 1404, 1407, 1527, 1713, 1792
GsaI	CCCAGC	2 cut(s)	445, 1629
GsuI	CTGGAG	1 cut(s)	1292
HaeIII	GGCC	7 cut(s)	253, 381, 446, 829, 1052, 1150, 1696
HapII	CCGG	1 cut(s)	1510
HgaI	GACGC	1 cut(s)	1200
Hin1II	CATG	4 cut(s)	176, 252, 994, 1129
HincII	GTYRAC	2 cut(s)	749, 1318
HindII	GTYRAC	2 cut(s)	749, 1318
HpaII	CCGG	1 cut(s)	1510
HphI	GGTGA	6 cut(s)	56, 434, 518, 539, 1731, 1913
Hpy166II	GTNNAC	6 cut(s)	95, 704, 749, 1123, 1318, 1493
Hpy8I	GTNNAC	6 cut(s)	95, 704, 749, 1123, 1318, 1493
Hpy99I	CGWCG	1 cut(s)	265
HpyAV	CCTTC	5 cut(s)	1101, 1161, 1362, 1721, 1832
HpyCH4III	ACNGT	5 cut(s)	848, 1289, 1562, 1672, 1897
HpyCH4IV	ACGT	3 cut(s)	260, 362, 706
HpyCH4V	TGCA	9 cut(s)	1019, 1087, 1145, 1207, 1346, 1526, 1791, 1847, 1871
HpyF10VI	GCNNNNNNNGC	9 cut(s)	198, 226, 519, 1001, 1136, 1213, 1217, 1430, 1523
HpySE526I	ACGT	3 cut(s)	260, 362, 706
Hsp92II	CATG	4 cut(s)	176, 252, 994, 1129
Ksp22I	TGATCA	1 cut(s)	286
Kzo9I	GATC	7 cut(s)	286, 466, 472, 601, 1267, 1360, 1883
LguI	GCTCTTC	1 cut(s)	15
LmnI	GCTCC	1 cut(s)	189
Lsp1109I	GCAGC	5 cut(s)	1249, 1390, 1393, 1538, 1803
LweI	GCATC	6 cut(s)	40, 1074, 1096, 1126, 1620, 1928
MaeI	CTAG	7 cut(s)	605, 1032, 1178, 1365, 1545, 1572, 1887
MaeII	ACGT	3 cut(s)	260, 362, 706
MaeIII	GTNAC	3 cut(s)	709, 1562, 1648
MalI	GATC	7 cut(s)	288, 468, 474, 603, 1269, 1362, 1885
MboI	GATC	7 cut(s)	286, 466, 472, 601, 1267, 1360, 1883
MboII	GAAGA	9 cut(s)	26, 29, 32, 537, 1616, 1740, 1765, 1778, 1847
MflI	RGATCY	2 cut(s)	601, 1883
MhlI	GDGCHC	1 cut(s)	248
MlyI	GAGTC	3 cut(s)	587, 950, 1798
MmeI	TCCRAC	3 cut(s)	570, 1081, 1449
Mph1103I	ATGCAT	1 cut(s)	1089
MroXI	GAANNNNTTC	1 cut(s)	1593
MseI	TTAA	5 cut(s)	81, 102, 717, 927, 1811
MslI	CAYNNNNRTG	1 cut(s)	1065
MspA1I	CMGCKG	4 cut(s)	137, 441, 522, 875
MspCI	CTTAAG	1 cut(s)	716
MspI	CCGG	1 cut(s)	1510
MspR9I	CCNGG	3 cut(s)	810, 1577, 1586
Mva1269I	GAATGC	1 cut(s)	1873
MvaI	CCWGG	3 cut(s)	810, 1577, 1586
MwoI	GCNNNNNNNGC	9 cut(s)	198, 226, 519, 1001, 1136, 1213, 1217, 1430, 1523
NdeI	CATATG	1 cut(s)	975
NdeII	GATC	7 cut(s)	286, 466, 472, 601, 1267, 1360, 1883
NlaIII	CATG	4 cut(s)	176, 252, 994, 1129
NlaIV	GGNNCC	2 cut(s)	900, 1257
NmuCI	GTSAC	1 cut(s)	1648
NsiI	ATGCAT	1 cut(s)	1089
NspV	TTCGAA	2 cut(s)	800, 1751
PceI	AGGCCT	1 cut(s)	1696
PciSI	GCTCTTC	1 cut(s)	15
PctI	GAATGC	1 cut(s)	1873
PdmI	GAANNNNTTC	1 cut(s)	1593
PfeI	GAWTC	8 cut(s)	125, 344, 856, 1075, 1388, 1607, 1736, 1850
PflMI	CCANNNNNTGG	2 cut(s)	662, 991
PkrI	GCNGC	6 cut(s)	1239, 1405, 1408, 1528, 1714, 1793
PleI	GAGTC	3 cut(s)	587, 949, 1798
PpsI	GAGTC	3 cut(s)	587, 949, 1798
Ppu21I	YACGTR	1 cut(s)	707
PpuMI	RGGWCCY	1 cut(s)	1841
Psp124BI	GAGCTC	1 cut(s)	248
Psp5II	RGGWCCY	1 cut(s)	1841
Psp6I	CCWGG	3 cut(s)	808, 1575, 1584
PspFI	CCCAGC	2 cut(s)	441, 1625
PspGI	CCWGG	3 cut(s)	808, 1575, 1584
PspN4I	GGNNCC	2 cut(s)	900, 1257
PspPI	GGNCC	6 cut(s)	380, 444, 763, 828, 1730, 1841
PspPPI	RGGWCCY	1 cut(s)	1841
PstI	CTGCAG	1 cut(s)	1849
PsuI	RGATCY	2 cut(s)	601, 1883
PvuII	CAGCTG	3 cut(s)	137, 441, 875
RsaI	GTAC	3 cut(s)	705, 845, 1894
RsaNI	GTAC	3 cut(s)	704, 844, 1893
RseI	CAYNNNNRTG	1 cut(s)	1065
SacI	GAGCTC	1 cut(s)	248
SalI	GTCGAC	1 cut(s)	1316
SapI	GCTCTTC	1 cut(s)	15
SaqAI	TTAA	5 cut(s)	81, 102, 717, 927, 1811
SatI	GCNGC	6 cut(s)	1238, 1404, 1407, 1527, 1713, 1792
Sau3AI	GATC	7 cut(s)	286, 466, 472, 601, 1267, 1360, 1883
Sau96I	GGNCC	6 cut(s)	380, 444, 763, 828, 1730, 1841
ScaI	AGTACT	1 cut(s)	1894
SchI	GAGTC	3 cut(s)	587, 950, 1798
ScrFI	CCNGG	3 cut(s)	810, 1577, 1586
SduI	GDGCHC	1 cut(s)	248
SfaNI	GCATC	6 cut(s)	40, 1074, 1096, 1126, 1620, 1928
SfcI	CTRYAG	3 cut(s)	436, 1260, 1845
SfuI	TTCGAA	2 cut(s)	800, 1751
SinI	GGWCC	3 cut(s)	763, 1730, 1841
SmiMI	CAYNNNNRTG	1 cut(s)	1065
SmlI	CTYRAG	2 cut(s)	597, 716
SmoI	CTYRAG	2 cut(s)	597, 716
SpeI	ACTAGT	1 cut(s)	1544
SseBI	AGGCCT	1 cut(s)	1696
SsiI	CCGC	4 cut(s)	522, 902, 1712, 1743
SspMI	CTAG	7 cut(s)	605, 1032, 1178, 1365, 1545, 1572, 1887
SstI	GAGCTC	1 cut(s)	248
StuI	AGGCCT	1 cut(s)	1696
StyD4I	CCNGG	3 cut(s)	808, 1575, 1584
StyI	CCWWGG	2 cut(s)	1333, 1924
TaaI	ACNGT	5 cut(s)	848, 1289, 1562, 1672, 1897
TaiI	ACGT	3 cut(s)	263, 365, 709
TaqI	TCGA	5 cut(s)	197, 475, 800, 1317, 1751
TatI	WGTACW	1 cut(s)	1892
TauI	GCSGC	1 cut(s)	1715
TfiI	GAWTC	8 cut(s)	125, 344, 856, 1075, 1388, 1607, 1736, 1850
Tru1I	TTAA	5 cut(s)	81, 102, 717, 927, 1811
Tru9I	TTAA	5 cut(s)	81, 102, 717, 927, 1811
TscAI	CASTG	1 cut(s)	1653
TseFI	GTSAC	1 cut(s)	1648
TseI	GCWGC	5 cut(s)	1237, 1403, 1406, 1526, 1791
Tsp45I	GTSAC	1 cut(s)	1648
TspDTI	ATGAA	4 cut(s)	383, 806, 1359, 1679
TspGWI	ACGGA	1 cut(s)	1498
TspRI	CASTG	1 cut(s)	1653
Van91I	CCANNNNNTGG	2 cut(s)	662, 991
Vha464I	CTTAAG	1 cut(s)	716
VpaK11BI	GGWCC	3 cut(s)	763, 1730, 1841
XapI	RAATTY	4 cut(s)	181, 1397, 1427, 1777
XmiI	GTMKAC	2 cut(s)	1317, 1492
XmnI	GAANNNNTTC	1 cut(s)	1593
XspI	CTAG	7 cut(s)	605, 1032, 1178, 1365, 1545, 1572, 1887
ZrmI	AGTACT	1 cut(s)	1894
Zsp2I	ATGCAT	1 cut(s)	1089

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.