AT3G60780

Protein of unknown function (DUF1442)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0015006
Physical Location & Seq
Forward (+)
22462613 .. 22463833
1221 bp
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UTR
Exon/CDS
Intron
AT3G60780.1

Sequence Viewer

Length: 657 bp
ATGAGGCTCGTCTGGTCGCCGGAAACTGCCTCTAACGCTTACATCCACACCGTTAGATCATGTAAAAGCTACAGAGACTCGAGCGTGGCGGAGTTTTTATCGGCTACGGCGGCGGGATGGAACACGAGGCTGATCGTTGAGACTTGGTCACGTGGAGATCCGATCGCAACTAGCGTCGGACTCGCCGTGGCAGCGATACACACATGTGGAAGACACGTGTGCATAGTTCCTGACGAAGAGTCGAGGTCGGAGTACGAGGCGGTGATGAGAGGAGCCGTTACGAGTGACTCAACGGAGGTTATGGTCTTAGACTCGGCGGAGGACGTGGTGGAGAGAATCTCCGGCGTGGATTTCATGGTGGTTGATTCGAAACGCCACGAGTTCGTAAACGCGTTGGGATTAGCGAAAACGAGCAAAATGGGCGCCGTTTTGGTGTGTAAAAACGCCACGCTGAAATCTATCCCTGGGTTTAAATGGCAAGGTCTTTTGAGACGAGGTACACGTGTCGTGAGATCGGTGTTCTTACCCGTCGGGAGAGGATTAGAGATCGTACACGTGGGAGCCAGTGGTGGTGGTAATGGTTTGAGGAAGATTCCTAGCCGTTGGATTAAACACATAGATCCTCGATCTGGAGAGGAACACTTGTTTAAGCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1442

Protein Analysis

218

Amino Acids

23.83

Weight (kDa)

9.32

Isoelectric Point (pI)

42.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1442 PF07279 1 - 218 1.5e-118 Protein of unknown function (DUF1442)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015006)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45360 AT3G60780
fragaria_vesca FvH4_7g11620
malus_domestica MD07G1120900.v1.1
prunus_persica Prupe.2G144100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0350051
rosa_laevigata RLG00000028503
rosa_multiflora Rmu_sc0003674.1_g000019
rosa_roxburghii Rroxscaffold_4G00305080
rosa_rugosa Rorug01G0208300
rosa_samantha Rh1AG224600 Rh1BG191900 Rh1CG209300 Rh1DG220400
rosa_wichuraiana Rw1G019190 Rw1G019310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 422
AccII CGCG 1 cut(s) 392
AciI CCGC 5 cut(s) 89, 110, 113, 260, 317
AclWI GGATC 2 cut(s) 152, 614
AcvI CACGTG 4 cut(s) 152, 217, 503, 556
AcyI GRCGYC 1 cut(s) 423
AfaI GTAC 3 cut(s) 254, 499, 552
AfiI CCNNNNNNNGG 1 cut(s) 629
AflIII ACRYGT 7 cut(s) 203, 214, 216, 390, 500, 502, 553
AhdI GACNNNNNGTC 1 cut(s) 238
AjiI CACGTC 1 cut(s) 325
AjnI CCWGG 1 cut(s) 463
AleI CACNNNNGTG 1 cut(s) 204
AluBI AGCT 1 cut(s) 69
AluI AGCT 1 cut(s) 69
Alw26I GTCTC 3 cut(s) 69, 134, 484
AlwI GGATC 2 cut(s) 152, 614
Ama87I CYCGRG 1 cut(s) 79
ApeKI GCWGC 1 cut(s) 191
AspLEI GCGC 1 cut(s) 425
AsuHPI GGTGA 1 cut(s) 274
AsuII TTCGAA 1 cut(s) 368
AvaI CYCGRG 1 cut(s) 79
BaeI ACNNNNGTAYC 2 cut(s) 489, 522
BanI GGYRCC 1 cut(s) 422
BauI CACGAG 2 cut(s) 124, 377
BbrPI CACGTG 4 cut(s) 152, 217, 503, 556
BbsI GAAGAC 1 cut(s) 217
BbvI GCAGC 1 cut(s) 203
BccI CCATC 1 cut(s) 111
BceAI ACGGC 5 cut(s) 123, 170, 260, 410, 585
BciT130I CCWGG 1 cut(s) 465
BcoDI GTCTC 3 cut(s) 69, 134, 484
BfaI CTAG 2 cut(s) 171, 597
BfmI CTRYAG 1 cut(s) 70
BfoI RGCGCY 1 cut(s) 426
BisI GCNGC 2 cut(s) 111, 192
BlsI GCNGC 2 cut(s) 112, 193
Bme1390I CCNGG 1 cut(s) 465
BmeRI GACNNNNNGTC 1 cut(s) 238
BmeT110I CYCGRG 1 cut(s) 79
BmgBI CACGTC 1 cut(s) 325
BmiI GGNNCC 3 cut(s) 274, 424, 562
BmrFI CCNGG 1 cut(s) 465
BpiI GAAGAC 1 cut(s) 217
BplI GAGNNNNNCTC 2 cut(s) 323, 355
BpmI CTGGAG 1 cut(s) 651
Bpu14I TTCGAA 1 cut(s) 368
BsaAI YACGTR 4 cut(s) 152, 217, 503, 556
BsaHI GRCGYC 1 cut(s) 423
BsaJI CCNNGG 3 cut(s) 186, 463, 464
Bsc4I CCNNNNNNNGG 1 cut(s) 629
Bse1I ACTGG 1 cut(s) 564
BseBI CCWGG 1 cut(s) 465
BseDI CCNNGG 3 cut(s) 186, 463, 464
BseGI GGATG 2 cut(s) 42, 122
BseLI CCNNNNNNNGG 1 cut(s) 629
BseNI ACTGG 1 cut(s) 564
BseRI GAGGAG 1 cut(s) 285
BseXI GCAGC 1 cut(s) 203
Bsh1236I CGCG 1 cut(s) 392
Bsh1285I CGRYCG 1 cut(s) 165
BshNI GGYRCC 1 cut(s) 422
BsiEI CGRYCG 1 cut(s) 165
BsiHKCI CYCGRG 1 cut(s) 79
BsiSI CCGG 2 cut(s) 20, 342
BslI CCNNNNNNNGG 1 cut(s) 629
BsmAI GTCTC 3 cut(s) 69, 134, 484
BsmBI CGTCTC 1 cut(s) 484
BsoBI CYCGRG 1 cut(s) 79
Bsp119I TTCGAA 1 cut(s) 368
Bsp143I GATC 8 cut(s) 56, 132, 157, 162, 512, 546, 619, 626
BspACI CCGC 5 cut(s) 89, 110, 113, 260, 317
BspFNI CGCG 1 cut(s) 392
BspLI GGNNCC 3 cut(s) 274, 424, 562
BspPI GGATC 2 cut(s) 152, 614
BspT104I TTCGAA 1 cut(s) 368
BspT107I GGYRCC 1 cut(s) 422
BsrI ACTGG 1 cut(s) 564
BssECI CCNNGG 3 cut(s) 186, 463, 464
BssMI GATC 8 cut(s) 56, 132, 157, 162, 512, 546, 619, 626
BssNI GRCGYC 1 cut(s) 423
BssSI CACGAG 2 cut(s) 124, 377
Bst2BI CACGAG 2 cut(s) 124, 377
Bst2UI CCWGG 1 cut(s) 465
Bst4CI ACNGT 1 cut(s) 52
Bst6I CTCTTC 1 cut(s) 231
BstACI GRCGYC 1 cut(s) 423
BstBAI YACGTR 4 cut(s) 152, 217, 503, 556
BstBI TTCGAA 1 cut(s) 368
BstDEI CTNAG 1 cut(s) 307
BstDSI CCRYGG 1 cut(s) 186
BstF5I GGATG 2 cut(s) 42, 122
BstFNI CGCG 1 cut(s) 392
BstH2I RGCGCY 1 cut(s) 426
BstHHI GCGC 1 cut(s) 425
BstKTI GATC 8 cut(s) 59, 135, 160, 165, 515, 549, 622, 629
BstMAI GTCTC 3 cut(s) 69, 134, 484
BstMBI GATC 8 cut(s) 56, 132, 157, 162, 512, 546, 619, 626
BstMCI CGRYCG 1 cut(s) 165
BstMWI GCNNNNNNNGC 4 cut(s) 35, 110, 191, 420
BstNI CCWGG 1 cut(s) 465
BstNSI RCATGY 1 cut(s) 207
BstSCI CCNGG 1 cut(s) 463
BstSFI CTRYAG 1 cut(s) 70
BstUI CGCG 1 cut(s) 392
BstV1I GCAGC 1 cut(s) 203
BstV2I GAAGAC 1 cut(s) 217
BstX2I RGATCY 2 cut(s) 157, 619
BstYI RGATCY 2 cut(s) 157, 619
BtgI CCRYGG 1 cut(s) 186
BtrI CACGTC 1 cut(s) 325
BtsCI GGATG 2 cut(s) 42, 122
BtsIMutI CAGTG 1 cut(s) 571
CfoI GCGC 1 cut(s) 425
CseI GACGC 1 cut(s) 163
Csp6I GTAC 3 cut(s) 253, 498, 551
CviAII CATG 3 cut(s) 60, 204, 355
CviJI RGCY 7 cut(s) 7, 69, 104, 130, 275, 563, 600
CviKI_1 RGCY 7 cut(s) 7, 69, 104, 130, 275, 563, 600
CviQI GTAC 3 cut(s) 253, 498, 551
DdeI CTNAG 1 cut(s) 307
DinI GGCGCC 1 cut(s) 424
DpnI GATC 8 cut(s) 58, 134, 159, 164, 514, 548, 621, 628
DpnII GATC 8 cut(s) 56, 132, 157, 162, 512, 546, 619, 626
DraI TTTAAA 1 cut(s) 472
DriI GACNNNNNGTC 1 cut(s) 238
Eam1104I CTCTTC 1 cut(s) 231
Eam1105I GACNNNNNGTC 1 cut(s) 238
EarI CTCTTC 1 cut(s) 231
EciI GGCGGA 2 cut(s) 104, 332
Eco72I CACGTG 4 cut(s) 152, 217, 503, 556
Eco88I CYCGRG 1 cut(s) 79
EcoRII CCWGG 1 cut(s) 463
EgeI GGCGCC 1 cut(s) 424
EheI GGCGCC 1 cut(s) 424
Esp3I CGTCTC 1 cut(s) 484
FaeI CATG 3 cut(s) 63, 207, 358
FaiI YATR 6 cut(s) 61, 205, 224, 302, 356, 617
FatI CATG 3 cut(s) 59, 203, 354
FauI CCCGC 1 cut(s) 106
Fnu4HI GCNGC 2 cut(s) 111, 192
FokI GGATG 2 cut(s) 29, 129
Fsp4HI GCNGC 2 cut(s) 111, 192
FspBI CTAG 2 cut(s) 171, 597
GlaI GCGC 1 cut(s) 424
GluI GCNGC 2 cut(s) 111, 192
GsuI CTGGAG 1 cut(s) 651
HaeII RGCGCY 1 cut(s) 426
HapII CCGG 2 cut(s) 20, 342
HgaI GACGC 1 cut(s) 163
HhaI GCGC 1 cut(s) 425
Hin1I GRCGYC 1 cut(s) 423
Hin1II CATG 3 cut(s) 63, 207, 358
Hin6I GCGC 1 cut(s) 423
HinP1I GCGC 1 cut(s) 423
HinfI GANTC 8 cut(s) 77, 180, 239, 287, 311, 336, 365, 592
HpaII CCGG 2 cut(s) 20, 342
HphI GGTGA 1 cut(s) 274
Hpy166II GTNNAC 3 cut(s) 388, 500, 553
Hpy188I TCNGA 3 cut(s) 162, 179, 250
Hpy188III TCNNGA 4 cut(s) 230, 508, 532, 630
Hpy8I GTNNAC 3 cut(s) 388, 500, 553
Hpy99I CGWCG 2 cut(s) 179, 533
HpyCH4III ACNGT 1 cut(s) 52
HpyCH4IV ACGT 5 cut(s) 151, 216, 324, 502, 555
HpyCH4V TGCA 1 cut(s) 222
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 110, 191, 420
HpyF3I CTNAG 1 cut(s) 307
HpySE526I ACGT 5 cut(s) 151, 216, 324, 502, 555
Hsp92I GRCGYC 1 cut(s) 423
Hsp92II CATG 3 cut(s) 63, 207, 358
HspAI GCGC 1 cut(s) 423
KasI GGCGCC 1 cut(s) 422
Kzo9I GATC 8 cut(s) 56, 132, 157, 162, 512, 546, 619, 626
LmnI GCTCC 2 cut(s) 272, 560
LpnPI CCDG 7 cut(s) 33, 243, 355, 450, 477, 577, 615
Lsp1109I GCAGC 1 cut(s) 203
MaeI CTAG 2 cut(s) 171, 597
MaeII ACGT 5 cut(s) 151, 216, 324, 502, 555
MaeIII GTNAC 3 cut(s) 147, 277, 284
MalI GATC 8 cut(s) 58, 134, 159, 164, 514, 548, 621, 628
MboI GATC 8 cut(s) 56, 132, 157, 162, 512, 546, 619, 626
MboII GAAGA 3 cut(s) 222, 248, 601
MflI RGATCY 2 cut(s) 157, 619
MluI ACGCGT 1 cut(s) 390
Mly113I GGCGCC 1 cut(s) 423
MlyI GAGTC 5 cut(s) 71, 174, 248, 281, 305
MmeI TCCRAC 3 cut(s) 157, 228, 584
MseI TTAA 3 cut(s) 471, 609, 648
MslI CAYNNNNRTG 1 cut(s) 204
MspI CCGG 2 cut(s) 20, 342
MspR9I CCNGG 1 cut(s) 465
MvaI CCWGG 1 cut(s) 465
MvnI CGCG 1 cut(s) 392
MwoI GCNNNNNNNGC 4 cut(s) 35, 110, 191, 420
NarI GGCGCC 1 cut(s) 423
NdeII GATC 8 cut(s) 56, 132, 157, 162, 512, 546, 619, 626
NlaIII CATG 3 cut(s) 63, 207, 358
NlaIV GGNNCC 3 cut(s) 274, 424, 562
NmeAIII GCCGAG 1 cut(s) 293
NmuCI GTSAC 2 cut(s) 147, 284
NspI RCATGY 1 cut(s) 207
NspV TTCGAA 1 cut(s) 368
OliI CACNNNNGTG 1 cut(s) 204
PaeR7I CTCGAG 1 cut(s) 79
PasI CCCWGGG 1 cut(s) 464
PciI ACATGT 1 cut(s) 203
PcsI WCGNNNNNNNCGW 3 cut(s) 171, 183, 499
PfeI GAWTC 3 cut(s) 336, 365, 592
PflFI GACNNNGTC 1 cut(s) 145
PkrI GCNGC 2 cut(s) 112, 193
Ple19I CGATCG 1 cut(s) 165
PleI GAGTC 5 cut(s) 71, 174, 247, 281, 305
PluTI GGCGCC 1 cut(s) 426
PmaCI CACGTG 4 cut(s) 152, 217, 503, 556
PmlI CACGTG 4 cut(s) 152, 217, 503, 556
PpsI GAGTC 5 cut(s) 71, 174, 247, 281, 305
Ppu21I YACGTR 4 cut(s) 152, 217, 503, 556
PscI ACATGT 1 cut(s) 203
Psp6I CCWGG 1 cut(s) 463
PspCI CACGTG 4 cut(s) 152, 217, 503, 556
PspGI CCWGG 1 cut(s) 463
PspN4I GGNNCC 3 cut(s) 274, 424, 562
PspXI VCTCGAGB 1 cut(s) 79
PsuI RGATCY 2 cut(s) 157, 619
PsyI GACNNNGTC 1 cut(s) 145
PvuI CGATCG 1 cut(s) 165
RsaI GTAC 3 cut(s) 254, 499, 552
RsaNI GTAC 3 cut(s) 253, 498, 551
RseI CAYNNNNRTG 1 cut(s) 204
SaqAI TTAA 3 cut(s) 471, 609, 648
SatI GCNGC 2 cut(s) 111, 192
Sau3AI GATC 8 cut(s) 56, 132, 157, 162, 512, 546, 619, 626
SchI GAGTC 5 cut(s) 71, 174, 248, 281, 305
ScrFI CCNGG 1 cut(s) 465
SfcI CTRYAG 1 cut(s) 70
SfoI GGCGCC 1 cut(s) 424
Sfr274I CTCGAG 1 cut(s) 79
SfuI TTCGAA 1 cut(s) 368
SlaI CTCGAG 1 cut(s) 79
SmiMI CAYNNNNRTG 1 cut(s) 204
SmlI CTYRAG 1 cut(s) 79
SmoI CTYRAG 1 cut(s) 79
SsiI CCGC 5 cut(s) 89, 110, 113, 260, 317
SspDI GGCGCC 1 cut(s) 422
SspMI CTAG 2 cut(s) 171, 597
StyD4I CCNGG 1 cut(s) 463
TaaI ACNGT 1 cut(s) 52
TaiI ACGT 5 cut(s) 154, 219, 327, 505, 558
TaqI TCGA 4 cut(s) 80, 242, 368, 625
TauI GCSGC 1 cut(s) 113
TfiI GAWTC 3 cut(s) 336, 365, 592
Tru1I TTAA 3 cut(s) 471, 609, 648
Tru9I TTAA 3 cut(s) 471, 609, 648
TscAI CASTG 1 cut(s) 571
TseFI GTSAC 2 cut(s) 147, 284
TseI GCWGC 1 cut(s) 191
Tsp45I GTSAC 2 cut(s) 147, 284
TspDTI ATGAA 1 cut(s) 343
TspGWI ACGGA 1 cut(s) 308
TspRI CASTG 1 cut(s) 571
Tth111I GACNNNGTC 1 cut(s) 145
XceI RCATGY 1 cut(s) 207
XhoI CTCGAG 1 cut(s) 79
XspI CTAG 2 cut(s) 171, 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.