AT4G00110

4-epimerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0008323
Physical Location & Seq
Reverse (-)
38480 .. 40298
1819 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G00110.1

Sequence Viewer

Length: 1293 bp
ATGAAGCAAATGTCGCATCTTGATGATATTCCTTCGACGCCTGGCAAGTTCAAGCCCTACTTCCACCGTACGCGGTGGCAATCCTCCGTCGCCAAGCTCGCCTTTTGGTCTCTCGTCTTCGTTGGTTTGATCTTCATCTTCTTCTACCGATCTCCCGTTTCCTCCAATCCCGCCGATCCTTCTCGCCGTTCTCTTCGTACCTACTCCTGGGGTGGACCCGCTTGGGAGAAACGGGTCAGATCCTCCGCCCGTCTCCGTACCCGCCGCGGTTTCTCTGTTCTCGTCACCGGAGCCGCCGGTTTCGTCGGTACCCATGTCTCCGCCGCTCTCAAACGCCGCGGAGATGGCGTTCTGGGGCTTGACAACTTCAACGATTACTACGATCCATCGCTCAAGAGAGCAAGGCAAGCCCTTTTGGAGAGAAGCGGAGTGTTTGTTGTTGAAGGAGACATCAATGACGCTGCTCTCTTGAAGAAGCTCTTTGAAGTTGTTCCCTTTACCCACGTTATGCATTTGGCTGCTCAAGCTGGTGTCAGGTACGCCATGGAAAATCCTAGCTCTTATGTTCACAGTAACATCGCTGGCTTCGTCAATCTCCTTGAGGTTTGCAAATCTGCCAATCCCCAACCCGCCATTGTCTGGGCTTCCTCTAGCTCTGTCTACGGGCTCAACACTAAGGTCCCCTTCTCCGAGAAAGACAGAACTGACCAACCAGCTAGTCTCTATGCTGCTACTAAGAAGGCTGGTGAAGAGATTGCTCACACTTACAATCACATCTATGGACTCTCTCTTACTGGCTTGAGGTTTTTCACCGTCTATGGTCCTTGGGGAAGGCCTGACATGGCCTATTTCTTCTTCACCAGAGACATTCTCAAAGGGAAAGCCATTTCAATCTTTGAGGGGGTCAACCATGGAACCGTGGCTAGGGACTTCACTTACATTGATGACATTGTCAAGGGGTGTTTAGGGGCTTTGGACACGGCTGAGAAGAGCACTGGTAGTGGTGGTAAGAAGCGCGGTGCGGCTCAGTTACGGGTTTTCAATCTCGGCAACACTTCGCCCGTTCCAGTGACTGATCTTGTCACCATTCTTGAGAGACTTCTCAAAGTCAAGGCCAAGAGGAACATTATGAAGTTGCCGAGGAACGGGGATGTGCAGTTCACTCATGCTAATATCAGTTCTGCGCAGAGAGAGCTCGGCTACAAACCCACCACCGATCTTCAGACGGGTCTCAAGAAGTTTGCGAGATGGTATTTGGGTTACTACAATGGCGGAAAGAAAGCTGCAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Epimerase GDP_Man_Dehyd

Protein Analysis

430

Amino Acids

47.69

Weight (kDa)

9.98

Isoelectric Point (pI)

43.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 93 - 330 5.4e-52 NAD dependent epimerase/dehydratase family
RmlD_sub_bind PF04321 93 - 257 5.7e-14 RmlD substrate binding domain
Polysacc_synt_2 PF02719 93 - 274 6.8e-09 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 94 - 413 1.9e-48 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 94 - 373 1.9e-13 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1185
Acc65I GGTACC 1 cut(s) 308
AccB1I GGYRCC 1 cut(s) 308
AccB7I CCANNNNNTGG 1 cut(s) 639
AccBSI CCGCTC 1 cut(s) 326
AccI GTMKAC 1 cut(s) 660
AccII CGCG 4 cut(s) 73, 267, 339, 1017
AclWI GGATC 3 cut(s) 170, 234, 377
AcuI CTGAAG 1 cut(s) 1205
AcyI GRCGYC 1 cut(s) 38
AfaI GTAC 5 cut(s) 70, 199, 259, 310, 539
AfiI CCNNNNNNNGG 4 cut(s) 207, 639, 924, 1145
AgsI TTSAA 7 cut(s) 52, 370, 443, 472, 485, 891, 1042
AjnI CCWGG 2 cut(s) 40, 206
AloI GAACNNNNNNTCC 4 cut(s) 333, 365, 1142, 1174
AluBI AGCT 9 cut(s) 97, 478, 527, 558, 654, 716, 1195, 1283, 1290
AluI AGCT 9 cut(s) 97, 478, 527, 558, 654, 716, 1195, 1283, 1290
Alw21I GWGCWC 2 cut(s) 995, 1197
Alw26I GTCTC 8 cut(s) 114, 257, 322, 441, 725, 858, 1090, 1235
AlwI GGATC 3 cut(s) 170, 234, 377
AlwNI CAGNNNCTG 1 cut(s) 1073
AoxI GGCC 3 cut(s) 833, 843, 1113
ApeKI GCWGC 4 cut(s) 461, 518, 728, 1283
Asp700I GAANNNNTTC 1 cut(s) 489
Asp718I GGTACC 1 cut(s) 308
AspLEI GCGC 2 cut(s) 1017, 1186
AspS9I GGNCC 3 cut(s) 215, 679, 821
AsuHPI GGTGA 5 cut(s) 277, 758, 802, 850, 1075
AvaII GGWCC 3 cut(s) 215, 679, 821
BaeI ACNNNNGTAYC 2 cut(s) 300, 333
BanI GGYRCC 1 cut(s) 308
BanII GRGCYC 2 cut(s) 669, 1197
BbsI GAAGAC 1 cut(s) 109
Bbv12I GWGCWC 2 cut(s) 995, 1197
BbvI GCAGC 4 cut(s) 448, 505, 715, 1270
BccI CCATC 3 cut(s) 338, 394, 1242
BceAI ACGGC 2 cut(s) 171, 996
BciT130I CCWGG 2 cut(s) 42, 208
BcoDI GTCTC 8 cut(s) 114, 257, 322, 441, 725, 858, 1090, 1235
BfaI CTAG 4 cut(s) 555, 651, 717, 924
BisI GCNGC 9 cut(s) 265, 294, 324, 337, 462, 519, 729, 1023, 1284
BlsI GCNGC 9 cut(s) 266, 295, 325, 338, 463, 520, 730, 1024, 1285
Bme1390I CCNGG 2 cut(s) 42, 208
Bme18I GGWCC 3 cut(s) 215, 679, 821
BmgT120I GGNCC 3 cut(s) 215, 679, 821
BmiI GGNNCC 5 cut(s) 217, 292, 310, 681, 916
BmrFI CCNGG 2 cut(s) 42, 208
BmsI GCATC 1 cut(s) 25
BpiI GAAGAC 1 cut(s) 109
BplI GAGNNNNNCTC 2 cut(s) 855, 887
BpuEI CTTGAG 6 cut(s) 377, 507, 620, 820, 1112, 1217
BsaBI GATNNNNATC 1 cut(s) 134
BsaHI GRCGYC 1 cut(s) 38
BsaI GGTCTC 2 cut(s) 114, 1235
BsaJI CCNNGG 8 cut(s) 207, 265, 337, 543, 824, 910, 918, 1139
BsaWI WCCGGW 1 cut(s) 287
BsaXI ACNNNNNCTCC 4 cut(s) 333, 363, 420, 450
Bsc4I CCNNNNNNNGG 4 cut(s) 207, 639, 924, 1145
Bse118I RCCGGY 1 cut(s) 296
Bse1I ACTGG 3 cut(s) 799, 1000, 1067
Bse8I GATNNNNATC 1 cut(s) 134
BseBI CCWGG 2 cut(s) 42, 208
BseDI CCNNGG 8 cut(s) 207, 265, 337, 543, 824, 910, 918, 1139
BseGI GGATG 1 cut(s) 1156
BseJI GATNNNNATC 1 cut(s) 134
BseLI CCNNNNNNNGG 4 cut(s) 207, 639, 924, 1145
BseMII CTCAG 2 cut(s) 975, 1040
BseNI ACTGG 3 cut(s) 799, 1000, 1067
BseXI GCAGC 4 cut(s) 448, 505, 715, 1270
BsgI GTGCAG 1 cut(s) 1175
Bsh1236I CGCG 4 cut(s) 73, 267, 339, 1017
BshFI GGCC 3 cut(s) 835, 845, 1115
BshNI GGYRCC 1 cut(s) 308
BsiHKAI GWGCWC 2 cut(s) 995, 1197
BsiSI CCGG 2 cut(s) 288, 297
BsiWI CGTACG 1 cut(s) 68
BslFI GGGAC 2 cut(s) 665, 941
BslI CCNNNNNNNGG 4 cut(s) 207, 639, 924, 1145
BsmAI GTCTC 8 cut(s) 114, 257, 322, 441, 725, 858, 1090, 1235
BsmBI CGTCTC 1 cut(s) 257
BsmFI GGGAC 2 cut(s) 665, 941
BsnI GGCC 3 cut(s) 835, 845, 1115
Bso31I GGTCTC 2 cut(s) 114, 1235
Bsp1286I GDGCHC 3 cut(s) 669, 995, 1197
Bsp143I GATC 7 cut(s) 129, 149, 175, 239, 382, 1075, 1216
Bsp19I CCATGG 2 cut(s) 543, 910
BspANI GGCC 3 cut(s) 835, 845, 1115
BspCNI CTCAG 2 cut(s) 976, 1039
BspFNI CGCG 4 cut(s) 73, 267, 339, 1017
BspLI GGNNCC 5 cut(s) 217, 292, 310, 681, 916
BspPI GGATC 3 cut(s) 170, 234, 377
BspQI GCTCTTC 1 cut(s) 983
BspT107I GGYRCC 1 cut(s) 308
BspTNI GGTCTC 2 cut(s) 114, 1235
BsrBI CCGCTC 1 cut(s) 326
BsrFI RCCGGY 1 cut(s) 296
BsrI ACTGG 3 cut(s) 799, 1000, 1067
BssAI RCCGGY 1 cut(s) 296
BssECI CCNNGG 8 cut(s) 207, 265, 337, 543, 824, 910, 918, 1139
BssMI GATC 7 cut(s) 129, 149, 175, 239, 382, 1075, 1216
BssNI GRCGYC 1 cut(s) 38
BssT1I CCWWGG 3 cut(s) 543, 824, 910
Bst2UI CCWGG 2 cut(s) 42, 208
Bst4CI ACNGT 4 cut(s) 68, 572, 814, 919
Bst6I CTCTTC 3 cut(s) 198, 744, 983
BstACI GRCGYC 1 cut(s) 38
BstC8I GCNNGC 4 cut(s) 99, 408, 583, 1288
BstDEI CTNAG 4 cut(s) 675, 735, 984, 1026
BstDSI CCRYGG 5 cut(s) 265, 337, 543, 910, 918
BstF5I GGATG 1 cut(s) 1156
BstFNI CGCG 4 cut(s) 73, 267, 339, 1017
BstHHI GCGC 2 cut(s) 1017, 1186
BstKTI GATC 7 cut(s) 132, 152, 178, 242, 385, 1078, 1219
BstMAI GTCTC 8 cut(s) 114, 257, 322, 441, 725, 858, 1090, 1235
BstMBI GATC 7 cut(s) 129, 149, 175, 239, 382, 1075, 1216
BstMWI GCNNNNNNNGC 6 cut(s) 13, 98, 345, 407, 524, 1192
BstNI CCWGG 2 cut(s) 42, 208
BstSCI CCNGG 2 cut(s) 40, 206
BstUI CGCG 4 cut(s) 73, 267, 339, 1017
BstV1I GCAGC 4 cut(s) 448, 505, 715, 1270
BstV2I GAAGAC 1 cut(s) 109
BstX2I RGATCY 1 cut(s) 239
BstYI RGATCY 1 cut(s) 239
BsuRI GGCC 3 cut(s) 835, 845, 1115
BtgI CCRYGG 5 cut(s) 265, 337, 543, 910, 918
BtgZI GCGATG 2 cut(s) 372, 562
BtsCI GGATG 1 cut(s) 1156
BtsIMutI CAGTG 2 cut(s) 993, 1074
Cac8I GCNNGC 4 cut(s) 99, 408, 583, 1288
CaiI CAGNNNCTG 1 cut(s) 1073
CfoI GCGC 2 cut(s) 1017, 1186
Cfr10I RCCGGY 1 cut(s) 296
Cfr13I GGNCC 3 cut(s) 215, 679, 821
Cfr42I CCGCGG 2 cut(s) 268, 340
CseI GACGC 2 cut(s) 46, 467
Csp6I GTAC 5 cut(s) 69, 198, 258, 309, 538
CviAII CATG 5 cut(s) 314, 544, 841, 911, 1166
CviQI GTAC 5 cut(s) 69, 198, 258, 309, 538
DdeI CTNAG 4 cut(s) 675, 735, 984, 1026
DpnI GATC 7 cut(s) 131, 151, 177, 241, 384, 1077, 1218
DpnII GATC 7 cut(s) 129, 149, 175, 239, 382, 1075, 1216
Eam1104I CTCTTC 3 cut(s) 198, 744, 983
EarI CTCTTC 3 cut(s) 198, 744, 983
EciI GGCGGA 3 cut(s) 235, 310, 1287
Ecl136II GAGCTC 1 cut(s) 1195
Eco130I CCWWGG 3 cut(s) 543, 824, 910
Eco147I AGGCCT 1 cut(s) 835
Eco24I GRGCYC 2 cut(s) 669, 1197
Eco31I GGTCTC 2 cut(s) 114, 1235
Eco47I GGWCC 3 cut(s) 215, 679, 821
Eco53kI GAGCTC 1 cut(s) 1195
Eco57I CTGAAG 1 cut(s) 1205
EcoICRI GAGCTC 1 cut(s) 1195
EcoO109I RGGNCCY 1 cut(s) 679
EcoRII CCWGG 2 cut(s) 40, 206
EcoT14I CCWWGG 3 cut(s) 543, 824, 910
EcoT22I ATGCAT 1 cut(s) 513
EcoT38I GRGCYC 2 cut(s) 669, 1197
ErhI CCWWGG 3 cut(s) 543, 824, 910
Esp3I CGTCTC 1 cut(s) 257
FaeI CATG 5 cut(s) 317, 547, 844, 914, 1169
FalI AAGNNNNNCTT 8 cut(s) 44, 76, 86, 118, 464, 496, 668, 700
FaqI GGGAC 2 cut(s) 665, 941
FatI CATG 5 cut(s) 313, 543, 840, 910, 1165
FauI CCCGC 4 cut(s) 178, 226, 269, 637
FblI GTMKAC 1 cut(s) 660
Fnu4HI GCNGC 9 cut(s) 265, 294, 324, 337, 462, 519, 729, 1023, 1284
FokI GGATG 1 cut(s) 1163
FriOI GRGCYC 2 cut(s) 669, 1197
Fsp4HI GCNGC 9 cut(s) 265, 294, 324, 337, 462, 519, 729, 1023, 1284
FspBI CTAG 4 cut(s) 555, 651, 717, 924
FspI TGCGCA 1 cut(s) 1185
GlaI GCGC 2 cut(s) 1016, 1185
GluI GCNGC 9 cut(s) 265, 294, 324, 337, 462, 519, 729, 1023, 1284
HaeIII GGCC 3 cut(s) 835, 845, 1115
HapII CCGG 2 cut(s) 288, 297
HgaI GACGC 2 cut(s) 46, 467
HhaI GCGC 2 cut(s) 1017, 1186
Hin1I GRCGYC 1 cut(s) 38
Hin1II CATG 5 cut(s) 317, 547, 844, 914, 1169
Hin6I GCGC 2 cut(s) 1015, 1184
HinP1I GCGC 2 cut(s) 1015, 1184
HincII GTYRAC 1 cut(s) 907
HindII GTYRAC 1 cut(s) 907
HinfI GANTC 1 cut(s) 783
HpaII CCGG 2 cut(s) 288, 297
HphI GGTGA 5 cut(s) 277, 758, 802, 850, 1075
Hpy166II GTNNAC 5 cut(s) 215, 568, 661, 907, 1161
Hpy188I TCNGA 3 cut(s) 239, 691, 1224
Hpy188III TCNNGA 5 cut(s) 20, 394, 469, 1091, 1234
Hpy8I GTNNAC 5 cut(s) 215, 568, 661, 907, 1161
Hpy99I CGWCG 3 cut(s) 40, 92, 308
HpyAV CCTTC 6 cut(s) 42, 189, 437, 694, 733, 825
HpyCH4III ACNGT 4 cut(s) 68, 572, 814, 919
HpyCH4IV ACGT 1 cut(s) 504
HpyCH4V TGCA 4 cut(s) 511, 609, 1156, 1286
HpyF10VI GCNNNNNNNGC 6 cut(s) 13, 98, 345, 407, 524, 1192
HpyF3I CTNAG 4 cut(s) 675, 735, 984, 1026
HpySE526I ACGT 1 cut(s) 504
Hsp92I GRCGYC 1 cut(s) 38
Hsp92II CATG 5 cut(s) 317, 547, 844, 914, 1169
HspAI GCGC 2 cut(s) 1015, 1184
KpnI GGTACC 1 cut(s) 312
KspI CCGCGG 2 cut(s) 268, 340
Kzo9I GATC 7 cut(s) 129, 149, 175, 239, 382, 1075, 1216
LguI GCTCTTC 1 cut(s) 983
LmnI GCTCC 1 cut(s) 290
Lsp1109I GCAGC 4 cut(s) 448, 505, 715, 1270
LweI GCATC 1 cut(s) 25
MaeI CTAG 4 cut(s) 555, 651, 717, 924
MaeII ACGT 1 cut(s) 504
MaeIII GTNAC 6 cut(s) 283, 572, 1029, 1069, 1081, 1259
MalI GATC 7 cut(s) 131, 151, 177, 241, 384, 1077, 1218
MbiI CCGCTC 1 cut(s) 326
MboI GATC 7 cut(s) 129, 149, 175, 239, 382, 1075, 1216
MflI RGATCY 1 cut(s) 239
MhlI GDGCHC 3 cut(s) 669, 995, 1197
MlyI GAGTC 1 cut(s) 777
MnlI CCTC 9 cut(s) 94, 172, 253, 595, 658, 795, 892, 1113, 1134
Mph1103I ATGCAT 1 cut(s) 513
MroXI GAANNNNTTC 1 cut(s) 489
MslI CAYNNNNRTG 2 cut(s) 21, 777
MspA1I CMGCKG 2 cut(s) 267, 339
MspI CCGG 2 cut(s) 288, 297
MspR9I CCNGG 2 cut(s) 42, 208
MvaI CCWGG 2 cut(s) 42, 208
MvnI CGCG 4 cut(s) 73, 267, 339, 1017
MwoI GCNNNNNNNGC 6 cut(s) 13, 98, 345, 407, 524, 1192
NcoI CCATGG 2 cut(s) 543, 910
NdeII GATC 7 cut(s) 129, 149, 175, 239, 382, 1075, 1216
NlaIII CATG 5 cut(s) 317, 547, 844, 914, 1169
NlaIV GGNNCC 5 cut(s) 217, 292, 310, 681, 916
NmeAIII GCCGAG 3 cut(s) 1026, 1164, 1176
NmuCI GTSAC 3 cut(s) 283, 1069, 1081
NsbI TGCGCA 1 cut(s) 1185
NsiI ATGCAT 1 cut(s) 513
PceI AGGCCT 1 cut(s) 835
PciSI GCTCTTC 1 cut(s) 983
PcsI WCGNNNNNNNCGW 2 cut(s) 378, 585
PdmI GAANNNNTTC 1 cut(s) 489
Pfl23II CGTACG 1 cut(s) 68
PflFI GACNNNGTC 1 cut(s) 950
PflMI CCANNNNNTGG 1 cut(s) 639
PkrI GCNGC 9 cut(s) 266, 295, 325, 338, 463, 520, 730, 1024, 1285
PleI GAGTC 1 cut(s) 777
PpsI GAGTC 1 cut(s) 777
PpuMI RGGWCCY 1 cut(s) 679
Psp124BI GAGCTC 1 cut(s) 1197
Psp5II RGGWCCY 1 cut(s) 679
Psp6I CCWGG 2 cut(s) 40, 206
PspGI CCWGG 2 cut(s) 40, 206
PspLI CGTACG 1 cut(s) 68
PspN4I GGNNCC 5 cut(s) 217, 292, 310, 681, 916
PspPI GGNCC 3 cut(s) 215, 679, 821
PspPPI RGGWCCY 1 cut(s) 679
PstNI CAGNNNCTG 1 cut(s) 1073
PsuI RGATCY 1 cut(s) 239
PsyI GACNNNGTC 1 cut(s) 950
RsaI GTAC 5 cut(s) 70, 199, 259, 310, 539
RsaNI GTAC 5 cut(s) 69, 198, 258, 309, 538
RseI CAYNNNNRTG 2 cut(s) 21, 777
SacI GAGCTC 1 cut(s) 1197
SacII CCGCGG 2 cut(s) 268, 340
SapI GCTCTTC 1 cut(s) 983
SatI GCNGC 9 cut(s) 265, 294, 324, 337, 462, 519, 729, 1023, 1284
Sau3AI GATC 7 cut(s) 129, 149, 175, 239, 382, 1075, 1216
Sau96I GGNCC 3 cut(s) 215, 679, 821
SchI GAGTC 1 cut(s) 777
ScrFI CCNGG 2 cut(s) 42, 208
SduI GDGCHC 3 cut(s) 669, 995, 1197
SfaNI GCATC 1 cut(s) 25
Sfr303I CCGCGG 2 cut(s) 268, 340
SgrBI CCGCGG 2 cut(s) 268, 340
SinI GGWCC 3 cut(s) 215, 679, 821
SmiMI CAYNNNNRTG 2 cut(s) 21, 777
SmlI CTYRAG 6 cut(s) 392, 522, 599, 799, 1091, 1232
SmoI CTYRAG 6 cut(s) 392, 522, 599, 799, 1091, 1232
SseBI AGGCCT 1 cut(s) 835
SspMI CTAG 4 cut(s) 555, 651, 717, 924
SstI GAGCTC 1 cut(s) 1197
StuI AGGCCT 1 cut(s) 835
StyD4I CCNGG 2 cut(s) 40, 206
StyI CCWWGG 3 cut(s) 543, 824, 910
TaaI ACNGT 4 cut(s) 68, 572, 814, 919
TaiI ACGT 1 cut(s) 507
TaqI TCGA 1 cut(s) 35
TauI GCSGC 5 cut(s) 267, 296, 326, 339, 1025
TscAI CASTG 2 cut(s) 1000, 1074
TseFI GTSAC 3 cut(s) 283, 1069, 1081
TseI GCWGC 4 cut(s) 461, 518, 728, 1283
Tsp45I GTSAC 3 cut(s) 283, 1069, 1081
TspDTI ATGAA 3 cut(s) 17, 124, 1145
TspGWI ACGGA 2 cut(s) 76, 245
TspRI CASTG 2 cut(s) 1000, 1074
Tth111I GACNNNGTC 1 cut(s) 950
Van91I CCANNNNNTGG 1 cut(s) 639
VpaK11BI GGWCC 3 cut(s) 215, 679, 821
XmiI GTMKAC 1 cut(s) 660
XmnI GAANNNNTTC 1 cut(s) 489
XspI CTAG 4 cut(s) 555, 651, 717, 924
Zsp2I ATGCAT 1 cut(s) 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.