pycom02g16850

UDP-glucuronate 4-epimerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0008323
Physical Location & Seq
Reverse (-)
14065626 .. 14066924
1299 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g16850.1

Sequence Viewer

Length: 1299 bp
ATGACCCATCTCGACAATAGTCCGCCGACGCCCGGAAAGTTCAAGATGGACAAGTCGCCGTACATTCACCGCCTCCGTTGGCACGGCTCGCTCGCCAAGCTCACGTTCTGGTCCTTCGTCTTCCTCGGCATGATCTTGATCTTCTTCTTCCGCTCGCCGTCCTCTAATTCTCTGCCATCCGATCCCTCCCGCCGCTCTCTCAGAACCTACAACTGGGGCGGACCCGCCTGGGAAAAACGTGTTAGGTCATCCGCCAGAGTCCGTTCCCGCCACGGAATCTCGGTCCTCGTCACTGGCGCGGCGGGCTTTGTCGGGACTCACGTGTCGGCGGCGCTCAAACGCCGCGGAGACGGGGTCCTTGGTCTGGATTGCTTCAATGACTACTACGACCCTTCGTTGAAGAGGGCTCGGCAAGCGCTTTTGGAGCGGAGTGGGGTGTTCATTGTGGAAGGCGACATAAACGACGTCACTTTGCTGAGCAAGCTCTTCGAGGTGGTGGCGTTCACCCATGTGATGCATTTGGCCGCCCAAGCCGGTGTGAGGTATGCCATGGAAAACCCAGGCTCATATGTTCATAGTAATATAGCTGGTCTTGTTAATCTTCTTGAAGTTTGTAAAAATGCAAATCCACAACCTGCAATTGTTTGGGCAAGTTCTAGTTCTGTTTATGGTTTGAATACTAAGGTACCCTTTTCGGAAAGAGACCGGACTGACCAGCCGGCTAGTCTCTATGCCGCTACCAAGAAAGCCGGTGAGGAAATTGCGCACACTTACAACCATATCTATGGCCTTTCCCTTACCGGATTGCGGTTCTTTACTGTTTATGGCCCCTGGGGAAGGCCTGATATGGCATACTTCTTTTTCACGAGGGATATATTGAAGGGGAAAACCATTCCAATCTTTGAGGCGGCTAATCATGGAACCGTTGCGAGGGATTTTACCTACATTGATGATATTGTGAAGGGATGCTTGGCGTCATTGGATACCGCTGAGAAGAGTACTGGGAGTGGGGGAAAGAAGAAGGGGCCTGCCCAATTGCGGGTTTTCAATTTGGGGAACACATCGCCTGTGCCAGTTACGGATCTTGTGACCATTTTGGAGAGGCTTTTGAAGGTAAAGGCTAAGAGAAATATAATGAAGTTGCCGCGTAATGGGGATGTTCAGTTTACGCATGCAAACATCAGTTCGGCTCAGAGGGAACTTGGGTATAAGCCCACAATAGATCTGCAGACAGGGCTGAAGAAATTTGTTCGGTGGTACCTCAGTTACTACTCTGGTGGGAAGAAGGCTTCTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Epimerase GDP_Man_Dehyd

Protein Analysis

433

Amino Acids

47.91

Weight (kDa)

9.91

Isoelectric Point (pI)

44.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 95 - 332 2.7e-50 NAD dependent epimerase/dehydratase family
RmlD_sub_bind PF04321 95 - 259 1.3e-12 RmlD substrate binding domain
Polysacc_synt_2 PF02719 95 - 279 7.9e-08 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 96 - 415 1.8e-46 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 96 - 368 8.5e-13 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 322
AatII GACGTC 1 cut(s) 468
Acc16I TGCGCA 1 cut(s) 765
Acc36I ACCTGC 1 cut(s) 643
Acc65I GGTACC 2 cut(s) 685, 1257
AccB1I GGYRCC 2 cut(s) 685, 1257
AccBSI CCGCTC 3 cut(s) 153, 195, 427
AccII CGCG 3 cut(s) 299, 345, 1147
AclWI GGATC 2 cut(s) 176, 1089
AcoI YGGCCR 1 cut(s) 522
AcsI RAATTY 1 cut(s) 1244
AcuI CTGAAG 1 cut(s) 1259
AcvI CACGTG 1 cut(s) 322
AcyI GRCGYC 3 cut(s) 29, 465, 974
AfaI GTAC 4 cut(s) 62, 687, 1000, 1259
AfeI AGCGCT 1 cut(s) 417
AflIII ACRYGT 2 cut(s) 238, 321
AgsI TTSAA 8 cut(s) 43, 376, 400, 608, 676, 880, 1048, 1111
AjnI CCWGG 3 cut(s) 227, 559, 830
AjuI GAANNNNNNNTTGG 4 cut(s) 89, 121, 953, 985
AleI CACNNNNGTG 1 cut(s) 509
AluBI AGCT 3 cut(s) 100, 484, 587
AluI AGCT 3 cut(s) 100, 484, 587
Alw26I GTCTC 3 cut(s) 342, 696, 731
AlwI GGATC 2 cut(s) 176, 1089
Aor51HI AGCGCT 1 cut(s) 417
AoxI GGCC 5 cut(s) 522, 787, 826, 839, 1025
ApoI RAATTY 1 cut(s) 1244
Asp718I GGTACC 2 cut(s) 685, 1257
AspLEI GCGC 4 cut(s) 299, 334, 418, 766
AspS9I GGNCC 6 cut(s) 111, 221, 283, 355, 827, 1025
AsuC2I CCSGG 1 cut(s) 33
AsuHPI GGTGA 3 cut(s) 59, 496, 764
AvaII GGWCC 4 cut(s) 111, 221, 283, 355
BaeI ACNNNNGTAYC 4 cut(s) 669, 702, 1249, 1282
BanI GGYRCC 2 cut(s) 685, 1257
BanII GRGCYC 1 cut(s) 409
BauI CACGAG 1 cut(s) 865
BbrPI CACGTG 1 cut(s) 322
BbsI GAAGAC 1 cut(s) 112
BccI CCATC 3 cut(s) 15, 40, 184
BceAI ACGGC 3 cut(s) 43, 100, 142
BcgI CGANNNNNNTGC 2 cut(s) 469, 503
BciT130I CCWGG 3 cut(s) 229, 561, 832
BciVI GTATCC 1 cut(s) 976
BcnI CCSGG 1 cut(s) 33
BcoDI GTCTC 3 cut(s) 342, 696, 731
BfaI CTAG 2 cut(s) 657, 723
BfmI CTRYAG 1 cut(s) 1226
BfoI RGCGCY 2 cut(s) 335, 419
BfuAI ACCTGC 1 cut(s) 643
BfuI GTATCC 1 cut(s) 976
BglII AGATCT 1 cut(s) 1222
BisI GCNGC 8 cut(s) 193, 300, 330, 343, 525, 735, 909, 1145
BlpI GCTNAGC 1 cut(s) 476
BlsI GCNGC 8 cut(s) 194, 301, 331, 344, 526, 736, 910, 1146
BmcAI AGTACT 1 cut(s) 1000
Bme1390I CCNGG 4 cut(s) 33, 229, 561, 832
Bme18I GGWCC 4 cut(s) 111, 221, 283, 355
BmgT120I GGNCC 6 cut(s) 111, 221, 283, 355, 827, 1025
BmiI GGNNCC 7 cut(s) 223, 356, 687, 829, 922, 1026, 1259
BmrFI CCNGG 4 cut(s) 33, 229, 561, 832
BmrI ACTGGG 2 cut(s) 223, 1011
BmsI GCATC 2 cut(s) 504, 956
BmuI ACTGGG 2 cut(s) 223, 1011
BoxI GACNNNNGTC 1 cut(s) 18
BpiI GAAGAC 1 cut(s) 112
Bpu1102I GCTNAGC 1 cut(s) 476
BpuMI CCSGG 1 cut(s) 33
BsaAI YACGTR 1 cut(s) 322
BsaBI GATNNNNATC 1 cut(s) 137
BsaHI GRCGYC 3 cut(s) 29, 465, 974
BsaI GGTCTC 1 cut(s) 696
BsaJI CCNNGG 9 cut(s) 124, 228, 271, 343, 358, 549, 559, 830, 831
BsaWI WCCGGW 2 cut(s) 705, 800
BsaXI ACNNNNNCTCC 2 cut(s) 339, 369
Bse118I RCCGGY 3 cut(s) 533, 718, 749
Bse1I ACTGG 4 cut(s) 218, 298, 1006, 1073
Bse8I GATNNNNATC 1 cut(s) 137
BseBI CCWGG 3 cut(s) 229, 561, 832
BseDI CCNNGG 9 cut(s) 124, 228, 271, 343, 358, 549, 559, 830, 831
BseGI GGATG 4 cut(s) 176, 248, 971, 1162
BseJI GATNNNNATC 1 cut(s) 137
BseMII CTCAG 5 cut(s) 214, 467, 981, 1205, 1276
BseNI ACTGG 4 cut(s) 218, 298, 1006, 1073
Bsh1236I CGCG 3 cut(s) 299, 345, 1147
BshFI GGCC 5 cut(s) 524, 789, 828, 841, 1027
BshNI GGYRCC 2 cut(s) 685, 1257
BsiSI CCGG 6 cut(s) 33, 534, 706, 719, 750, 801
BslFI GGGAC 1 cut(s) 328
BsmAI GTCTC 3 cut(s) 342, 696, 731
BsmBI CGTCTC 1 cut(s) 342
BsmFI GGGAC 1 cut(s) 328
BsnI GGCC 5 cut(s) 524, 789, 828, 841, 1027
Bso31I GGTCTC 1 cut(s) 696
Bsp1286I GDGCHC 1 cut(s) 409
Bsp143I GATC 5 cut(s) 132, 138, 181, 1081, 1222
Bsp1720I GCTNAGC 1 cut(s) 476
Bsp19I CCATGG 1 cut(s) 549
BspANI GGCC 5 cut(s) 524, 789, 828, 841, 1027
BspCNI CTCAG 5 cut(s) 213, 468, 982, 1204, 1275
BspFNI CGCG 3 cut(s) 299, 345, 1147
BspLI GGNNCC 7 cut(s) 223, 356, 687, 829, 922, 1026, 1259
BspMAI CTGCAG 1 cut(s) 1230
BspMI ACCTGC 1 cut(s) 643
BspPI GGATC 2 cut(s) 176, 1089
BspQI GCTCTTC 1 cut(s) 491
BspT107I GGYRCC 2 cut(s) 685, 1257
BspTNI GGTCTC 1 cut(s) 696
BsrBI CCGCTC 3 cut(s) 153, 195, 427
BsrFI RCCGGY 3 cut(s) 533, 718, 749
BsrI ACTGG 4 cut(s) 218, 298, 1006, 1073
BssAI RCCGGY 3 cut(s) 533, 718, 749
BssECI CCNNGG 9 cut(s) 124, 228, 271, 343, 358, 549, 559, 830, 831
BssMI GATC 5 cut(s) 132, 138, 181, 1081, 1222
BssNI GRCGYC 3 cut(s) 29, 465, 974
BssSI CACGAG 1 cut(s) 865
BssT1I CCWWGG 2 cut(s) 358, 549
Bst2BI CACGAG 1 cut(s) 865
Bst2UI CCWGG 3 cut(s) 229, 561, 832
Bst4CI ACNGT 2 cut(s) 820, 925
Bst6I CTCTTC 3 cut(s) 395, 491, 989
BstACI GRCGYC 3 cut(s) 29, 465, 974
BstBAI YACGTR 1 cut(s) 322
BstC8I GCNNGC 9 cut(s) 89, 93, 155, 304, 414, 482, 720, 1029, 1173
BstDEI CTNAG 7 cut(s) 200, 476, 681, 990, 1122, 1191, 1262
BstDSI CCRYGG 3 cut(s) 271, 343, 549
BstENI CCTNNNNNAGG 1 cut(s) 835
BstF5I GGATG 4 cut(s) 176, 248, 971, 1162
BstFNI CGCG 3 cut(s) 299, 345, 1147
BstH2I RGCGCY 2 cut(s) 335, 419
BstHHI GCGC 4 cut(s) 299, 334, 418, 766
BstKTI GATC 5 cut(s) 135, 141, 184, 1084, 1225
BstMAI GTCTC 3 cut(s) 342, 696, 731
BstMBI GATC 5 cut(s) 132, 138, 181, 1081, 1222
BstMWI GCNNNNNNNGC 8 cut(s) 88, 97, 303, 413, 424, 481, 530, 1234
BstNI CCWGG 3 cut(s) 229, 561, 832
BstNSI RCATGY 1 cut(s) 1175
BstPAI GACNNNNGTC 1 cut(s) 18
BstSCI CCNGG 4 cut(s) 31, 227, 559, 830
BstSFI CTRYAG 1 cut(s) 1226
BstUI CGCG 3 cut(s) 299, 345, 1147
BstV2I GAAGAC 1 cut(s) 112
BstX2I RGATCY 2 cut(s) 1081, 1222
BstXI CCANNNNNNTGG 1 cut(s) 785
BstYI RGATCY 2 cut(s) 1081, 1222
BsuI GTATCC 1 cut(s) 976
BsuRI GGCC 5 cut(s) 524, 789, 828, 841, 1027
BtgI CCRYGG 3 cut(s) 271, 343, 549
BtgZI GCGATG 1 cut(s) 1047
BtsCI GGATG 4 cut(s) 176, 248, 971, 1162
BtsIMutI CAGTG 1 cut(s) 291
BveI ACCTGC 1 cut(s) 643
Cac8I GCNNGC 9 cut(s) 89, 93, 155, 304, 414, 482, 720, 1029, 1173
CfoI GCGC 4 cut(s) 299, 334, 418, 766
Cfr10I RCCGGY 3 cut(s) 533, 718, 749
Cfr13I GGNCC 6 cut(s) 111, 221, 283, 355, 827, 1025
Cfr42I CCGCGG 1 cut(s) 346
CseI GACGC 2 cut(s) 37, 963
Csp6I GTAC 4 cut(s) 61, 686, 999, 1258
CviAII CATG 5 cut(s) 130, 509, 550, 917, 1172
CviQI GTAC 4 cut(s) 61, 686, 999, 1258
DdeI CTNAG 7 cut(s) 200, 476, 681, 990, 1122, 1191, 1262
DpnI GATC 5 cut(s) 134, 140, 183, 1083, 1224
DpnII GATC 5 cut(s) 132, 138, 181, 1081, 1222
DrdI GACNNNNNNGTC 1 cut(s) 322
DseDI GACNNNNNNGTC 1 cut(s) 322
EaeI YGGCCR 1 cut(s) 522
Eam1104I CTCTTC 3 cut(s) 395, 491, 989
EarI CTCTTC 3 cut(s) 395, 491, 989
EciI GGCGGA 3 cut(s) 12, 234, 241
Eco130I CCWWGG 2 cut(s) 358, 549
Eco147I AGGCCT 1 cut(s) 841
Eco24I GRGCYC 1 cut(s) 409
Eco31I GGTCTC 1 cut(s) 696
Eco47I GGWCC 4 cut(s) 111, 221, 283, 355
Eco47III AGCGCT 1 cut(s) 417
Eco57I CTGAAG 1 cut(s) 1259
Eco72I CACGTG 1 cut(s) 322
EcoNI CCTNNNNNAGG 1 cut(s) 835
EcoO109I RGGNCCY 2 cut(s) 355, 1025
EcoRII CCWGG 3 cut(s) 227, 559, 830
EcoT14I CCWWGG 2 cut(s) 358, 549
EcoT22I ATGCAT 1 cut(s) 519
EcoT38I GRGCYC 1 cut(s) 409
ErhI CCWWGG 2 cut(s) 358, 549
Esp3I CGTCTC 1 cut(s) 342
FaeI CATG 5 cut(s) 133, 512, 553, 920, 1175
FalI AAGNNNNNCTT 4 cut(s) 674, 706, 953, 985
FaqI GGGAC 1 cut(s) 328
FatI CATG 5 cut(s) 129, 508, 549, 916, 1171
FauI CCCGC 5 cut(s) 197, 232, 275, 295, 1032
FauNDI CATATG 1 cut(s) 568
Fnu4HI GCNGC 8 cut(s) 193, 300, 330, 343, 525, 735, 909, 1145
FokI GGATG 4 cut(s) 163, 235, 978, 1169
FriOI GRGCYC 1 cut(s) 409
Fsp4HI GCNGC 8 cut(s) 193, 300, 330, 343, 525, 735, 909, 1145
FspBI CTAG 2 cut(s) 657, 723
FspI TGCGCA 1 cut(s) 765
GlaI GCGC 4 cut(s) 298, 333, 417, 765
GluI GCNGC 8 cut(s) 193, 300, 330, 343, 525, 735, 909, 1145
HaeII RGCGCY 2 cut(s) 335, 419
HaeIII GGCC 5 cut(s) 524, 789, 828, 841, 1027
HapII CCGG 6 cut(s) 33, 534, 706, 719, 750, 801
HgaI GACGC 2 cut(s) 37, 963
HhaI GCGC 4 cut(s) 299, 334, 418, 766
Hin1I GRCGYC 3 cut(s) 29, 465, 974
Hin1II CATG 5 cut(s) 133, 512, 553, 920, 1175
Hin6I GCGC 4 cut(s) 297, 332, 416, 764
HinP1I GCGC 4 cut(s) 297, 332, 416, 764
HinfI GANTC 3 cut(s) 258, 276, 316
HpaII CCGG 6 cut(s) 33, 534, 706, 719, 750, 801
HphI GGTGA 3 cut(s) 59, 496, 764
Hpy166II GTNNAC 2 cut(s) 504, 1167
Hpy188I TCNGA 4 cut(s) 181, 203, 697, 1194
Hpy188III TCNNGA 8 cut(s) 11, 43, 136, 313, 365, 605, 865, 1293
Hpy8I GTNNAC 2 cut(s) 504, 1167
Hpy99I CGWCG 2 cut(s) 31, 467
HpyAV CCTTC 9 cut(s) 124, 402, 443, 831, 874, 955, 1015, 1105, 1279
HpyCH4III ACNGT 2 cut(s) 820, 925
HpyCH4IV ACGT 4 cut(s) 104, 238, 321, 465
HpyCH4V TGCA 5 cut(s) 517, 623, 638, 1175, 1228
HpyF10VI GCNNNNNNNGC 8 cut(s) 88, 97, 303, 413, 424, 481, 530, 1234
HpyF3I CTNAG 7 cut(s) 200, 476, 681, 990, 1122, 1191, 1262
HpySE526I ACGT 4 cut(s) 104, 238, 321, 465
Hsp92I GRCGYC 3 cut(s) 29, 465, 974
Hsp92II CATG 5 cut(s) 133, 512, 553, 920, 1175
HspAI GCGC 4 cut(s) 297, 332, 416, 764
KpnI GGTACC 2 cut(s) 689, 1261
KroI GCCGGC 1 cut(s) 718
KroNI GCCGGC 1 cut(s) 720
KspI CCGCGG 1 cut(s) 346
Kzo9I GATC 5 cut(s) 132, 138, 181, 1081, 1222
LguI GCTCTTC 1 cut(s) 491
LmnI GCTCC 1 cut(s) 424
LweI GCATC 2 cut(s) 504, 956
MaeI CTAG 2 cut(s) 657, 723
MaeII ACGT 4 cut(s) 104, 238, 321, 465
MaeIII GTNAC 5 cut(s) 289, 466, 1075, 1087, 1265
MalI GATC 5 cut(s) 134, 140, 183, 1083, 1224
MbiI CCGCTC 3 cut(s) 153, 195, 427
MboI GATC 5 cut(s) 132, 138, 181, 1081, 1222
MfeI CAATTG 2 cut(s) 639, 1034
MflI RGATCY 2 cut(s) 1081, 1222
MhlI GDGCHC 1 cut(s) 409
MluCI AATT 6 cut(s) 166, 639, 759, 1034, 1048, 1244
MlyI GAGTC 2 cut(s) 267, 310
Mph1103I ATGCAT 1 cut(s) 519
MroNI GCCGGC 1 cut(s) 718
MseI TTAA 1 cut(s) 597
MslI CAYNNNNRTG 2 cut(s) 509, 783
MspA1I CMGCKG 2 cut(s) 345, 989
MspI CCGG 6 cut(s) 33, 534, 706, 719, 750, 801
MspR9I CCNGG 4 cut(s) 33, 229, 561, 832
MunI CAATTG 2 cut(s) 639, 1034
MvaI CCWGG 3 cut(s) 229, 561, 832
MvnI CGCG 3 cut(s) 299, 345, 1147
MwoI GCNNNNNNNGC 8 cut(s) 88, 97, 303, 413, 424, 481, 530, 1234
NaeI GCCGGC 1 cut(s) 720
NciI CCSGG 1 cut(s) 33
NcoI CCATGG 1 cut(s) 549
NdeI CATATG 1 cut(s) 568
NdeII GATC 5 cut(s) 132, 138, 181, 1081, 1222
NgoMIV GCCGGC 1 cut(s) 718
NlaIII CATG 5 cut(s) 133, 512, 553, 920, 1175
NlaIV GGNNCC 7 cut(s) 223, 356, 687, 829, 922, 1026, 1259
NmeAIII GCCGAG 2 cut(s) 105, 388
NmuCI GTSAC 3 cut(s) 289, 466, 1087
NsbI TGCGCA 1 cut(s) 765
NsiI ATGCAT 1 cut(s) 519
NspI RCATGY 1 cut(s) 1175
OliI CACNNNNGTG 1 cut(s) 509
PaeI GCATGC 1 cut(s) 1175
PasI CCCWGGG 1 cut(s) 831
PceI AGGCCT 1 cut(s) 841
PciSI GCTCTTC 1 cut(s) 491
PcsI WCGNNNNNNNCGW 1 cut(s) 318
PdiI GCCGGC 1 cut(s) 720
PfeI GAWTC 1 cut(s) 276
PflFI GACNNNGTC 1 cut(s) 353
PkrI GCNGC 8 cut(s) 194, 301, 331, 344, 526, 736, 910, 1146
PleI GAGTC 2 cut(s) 266, 310
PmaCI CACGTG 1 cut(s) 322
PmlI CACGTG 1 cut(s) 322
PpsI GAGTC 2 cut(s) 266, 310
Ppu21I YACGTR 1 cut(s) 322
PpuMI RGGWCCY 1 cut(s) 355
PshAI GACNNNNGTC 1 cut(s) 18
Psp5II RGGWCCY 1 cut(s) 355
Psp6I CCWGG 3 cut(s) 227, 559, 830
PspCI CACGTG 1 cut(s) 322
PspGI CCWGG 3 cut(s) 227, 559, 830
PspN4I GGNNCC 7 cut(s) 223, 356, 687, 829, 922, 1026, 1259
PspPI GGNCC 6 cut(s) 111, 221, 283, 355, 827, 1025
PspPPI RGGWCCY 1 cut(s) 355
PstI CTGCAG 1 cut(s) 1230
PsuI RGATCY 2 cut(s) 1081, 1222
PsyI GACNNNGTC 1 cut(s) 353
RsaI GTAC 4 cut(s) 62, 687, 1000, 1259
RsaNI GTAC 4 cut(s) 61, 686, 999, 1258
RseI CAYNNNNRTG 2 cut(s) 509, 783
SacII CCGCGG 1 cut(s) 346
SapI GCTCTTC 1 cut(s) 491
SaqAI TTAA 1 cut(s) 597
SatI GCNGC 8 cut(s) 193, 300, 330, 343, 525, 735, 909, 1145
Sau3AI GATC 5 cut(s) 132, 138, 181, 1081, 1222
Sau96I GGNCC 6 cut(s) 111, 221, 283, 355, 827, 1025
ScaI AGTACT 1 cut(s) 1000
SchI GAGTC 2 cut(s) 267, 310
ScrFI CCNGG 4 cut(s) 33, 229, 561, 832
SduI GDGCHC 1 cut(s) 409
SfaNI GCATC 2 cut(s) 504, 956
SfcI CTRYAG 1 cut(s) 1226
Sfr303I CCGCGG 1 cut(s) 346
SgrBI CCGCGG 1 cut(s) 346
SinI GGWCC 4 cut(s) 111, 221, 283, 355
SmiMI CAYNNNNRTG 2 cut(s) 509, 783
SphI GCATGC 1 cut(s) 1175
Sse9I AATT 6 cut(s) 166, 639, 759, 1034, 1048, 1244
SseBI AGGCCT 1 cut(s) 841
SspMI CTAG 2 cut(s) 657, 723
StuI AGGCCT 1 cut(s) 841
StyD4I CCNGG 4 cut(s) 31, 227, 559, 830
StyI CCWWGG 2 cut(s) 358, 549
TaaI ACNGT 2 cut(s) 820, 925
TaiI ACGT 4 cut(s) 107, 241, 324, 468
TaqI TCGA 2 cut(s) 12, 489
TaqII GACCGA 1 cut(s) 271
TasI AATT 6 cut(s) 166, 639, 759, 1034, 1048, 1244
TatI WGTACW 1 cut(s) 998
TauI GCSGC 8 cut(s) 195, 302, 332, 345, 527, 737, 911, 1147
TfiI GAWTC 1 cut(s) 276
Tru1I TTAA 1 cut(s) 597
Tru9I TTAA 1 cut(s) 597
TscAI CASTG 1 cut(s) 298
TseFI GTSAC 3 cut(s) 289, 466, 1087
Tsp45I GTSAC 3 cut(s) 289, 466, 1087
TspDTI ATGAA 3 cut(s) 430, 563, 1151
TspGWI ACGGA 4 cut(s) 65, 251, 288, 1094
TspRI CASTG 1 cut(s) 298
Tth111I GACNNNGTC 1 cut(s) 353
VpaK11BI GGWCC 4 cut(s) 111, 221, 283, 355
XagI CCTNNNNNAGG 1 cut(s) 835
XapI RAATTY 1 cut(s) 1244
XceI RCATGY 1 cut(s) 1175
XspI CTAG 2 cut(s) 657, 723
ZraI GACGTC 1 cut(s) 466
ZrmI AGTACT 1 cut(s) 1000
Zsp2I ATGCAT 1 cut(s) 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.