Rw1G019130

UDP-glucuronate 4-epimerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0008323
Physical Location & Seq
Forward (+)
41850860 .. 41852164
1305 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G019130.1

Sequence Viewer

Length: 1305 bp
ATGACCCAGCTCAAGCAAATGTCTCATCTTGACAATGGCCCCCCAACTCCAGGCAAGTTCAAGCCCTACATTCACAGAGTCCGATGGCACAGCTCCCTCGCCAAGCTCACATTGTGGTCCTTCGTCTTCTTGGCCCTGATCTTGCTCTTCTTTTTCCGGTCGCCGTCGTCCAATTCCCTCCCCTCCGATCCATCCCGCCGCTCCCTCAGAACCTACAACTGGGGCGGACCCGCCTGGGAGAAACGGGTCCGGTCCTCCGCCCGGGTCCGGTCCAGAAACGGCATGTCGGTGCTCGTCACCGGCGCCGCCGGGTTCGTCGGCACCCACGTCTCCGCCGCCCTCAAGCGCCGGGGCGACGGCGTTTTGGGGCTGGACAGCTTCAATGACTACTACGACCCGTCGTTGAAGAGAGCGAGGCAGGCGCTTTTGGAGCGCAGTGGGGTGTTCATTGTGGAAGGGGACATAAACGACTCGGCGTTGCTGAGCAAGCTTTTTGAGGTGGTGGCGTTTACTCATGTCATGCATTTGGCTGCTCAAGCTGGTGTGAGGTATGCCATGGAGAACCCCGGTTCATATGTTCATAGTAATATAGCTGGTCTTGTTAATCTGCTTGAAGTTTGTAAGAATGCAAATCCACAACCTGCGATTGTTTGGGCTAGCTCTAGTTCTGTCTATGGACTTAACACTAAGGTACCCTTTTCGGAGAGAGACCGGACTGATCAGCCTGCTAGTTTGTATGCTGCTACTAAGAAAGCTGGTGAAGAGATTGCACATACTTATAATCATATCTATGGTCTTTCGCTTACCGGGTTGAGGTTCTTTACAGTATATGGTCCTTGGGGGAGGCCTGACATGGCTTACTTCTTTTTCACGAGGGATATATTGAAGGGGAAGACTATTCCGATTTTTGAAGCGGCTAATCACGGCACTGTGGCTAGGGATTTTACCTACATTGATGATATTGTCAAGGGCTGCTTGGCGTCGTTGGACACTGCGGAGAAGAGCACCGGGAGTGGGGGGAAGAAGAAGGGTCCTGCCCAGTTGAGGGTTTTCAATTTGGGGAATACGTCTCCTGTGCCAGTTTCGGATCTTGTCACCATTTTGGAGAGGCTTTTGAAGGTCAAGGCCAAGAGGAACATATTGAAGTTGCCGCGTAATGGGGATGTTCAGTTTACTCATGCAAACATCAGCTCCGCGCAGAGGGAGCTTGGGTATAAGCCCACAACCGATCTGCAGACAGGGTTGAAGAAATTCGTTAGGTGGTACCTGACTTACTATTCTGGTGGGAAGAAGGCCGCAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Epimerase GDP_Man_Dehyd

Protein Analysis

434

Amino Acids

48.0

Weight (kDa)

9.98

Isoelectric Point (pI)

45.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 95 - 261 9.1e-14 RmlD substrate binding domain
Epimerase PF01370 97 - 334 1.2e-51 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 97 - 276 6.6e-09 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 98 - 417 4.4e-47 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 98 - 325 3.6e-13 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 780
Acc36I ACCTGC 1 cut(s) 649
Acc65I GGTACC 2 cut(s) 691, 1263
AccB1I GGYRCC 4 cut(s) 302, 320, 691, 1263
AccBSI CCGCTC 1 cut(s) 201
AccII CGCG 2 cut(s) 1153, 1196
AclWI GGATC 2 cut(s) 182, 1095
AcsI RAATTY 1 cut(s) 1250
AcyI GRCGYC 2 cut(s) 303, 980
AdeI CACNNNGTG 1 cut(s) 114
AfaI GTAC 2 cut(s) 693, 1265
AjiI CACGTC 1 cut(s) 328
AjnI CCWGG 2 cut(s) 49, 233
Alw21I GWGCWC 2 cut(s) 294, 1007
Alw26I GTCTC 4 cut(s) 27, 334, 702, 1074
AlwI GGATC 2 cut(s) 182, 1095
Ama87I CYCGRG 1 cut(s) 261
AoxI GGCC 5 cut(s) 37, 132, 845, 1125, 1293
ApeKI GCWGC 3 cut(s) 530, 740, 972
ApoI RAATTY 1 cut(s) 1250
Asp700I GAANNNNTTC 1 cut(s) 1250
Asp718I GGTACC 2 cut(s) 691, 1263
AspLEI GCGC 5 cut(s) 305, 348, 424, 435, 1198
AsuC2I CCSGG 7 cut(s) 262, 263, 310, 350, 567, 808, 1009
AsuHPI GGTGA 3 cut(s) 289, 770, 1087
AsuNHI GCTAGC 1 cut(s) 656
AvaI CYCGRG 1 cut(s) 261
AvaII GGWCC 8 cut(s) 117, 227, 247, 252, 265, 270, 833, 1031
BaeI ACNNNNGTAYC 2 cut(s) 675, 708
BanI GGYRCC 4 cut(s) 302, 320, 691, 1263
BauI CACGAG 1 cut(s) 871
BbsI GAAGAC 2 cut(s) 118, 899
Bbv12I GWGCWC 2 cut(s) 294, 1007
BbvI GCAGC 3 cut(s) 517, 727, 959
BccI CCATC 2 cut(s) 78, 199
BceAI ACGGC 4 cut(s) 148, 295, 373, 940
BciT130I CCWGG 2 cut(s) 51, 235
BclI TGATCA 1 cut(s) 718
BcnI CCSGG 7 cut(s) 262, 263, 310, 350, 567, 808, 1009
BcoDI GTCTC 4 cut(s) 27, 334, 702, 1074
BfaI CTAG 4 cut(s) 657, 663, 729, 936
BfmI CTRYAG 1 cut(s) 1232
BfoI RGCGCY 3 cut(s) 306, 349, 425
BfuAI ACCTGC 1 cut(s) 649
BisI GCNGC 9 cut(s) 199, 306, 336, 531, 741, 915, 973, 1151, 1296
BlpI GCTNAGC 1 cut(s) 482
BlsI GCNGC 9 cut(s) 200, 307, 337, 532, 742, 916, 974, 1152, 1297
Bme1390I CCNGG 9 cut(s) 51, 235, 262, 263, 310, 350, 567, 808, 1009
Bme18I GGWCC 8 cut(s) 117, 227, 247, 252, 265, 270, 833, 1031
BmeT110I CYCGRG 1 cut(s) 261
BmgBI CACGTC 1 cut(s) 328
BmiI GGNNCC 9 cut(s) 40, 229, 248, 266, 304, 322, 693, 1032, 1265
BmrFI CCNGG 9 cut(s) 51, 235, 262, 263, 310, 350, 567, 808, 1009
BmrI ACTGGG 2 cut(s) 229, 1033
BmtI GCTAGC 1 cut(s) 660
BmuI ACTGGG 2 cut(s) 229, 1033
BpiI GAAGAC 2 cut(s) 118, 899
BpmI CTGGAG 1 cut(s) 33
Bpu1102I GCTNAGC 1 cut(s) 482
BpuEI CTTGAG 2 cut(s) 326, 519
BpuMI CCSGG 7 cut(s) 262, 263, 310, 350, 567, 808, 1009
BsaHI GRCGYC 2 cut(s) 303, 980
BsaI GGTCTC 1 cut(s) 702
BsaJI CCNNGG 6 cut(s) 234, 261, 349, 555, 565, 836
BsaWI WCCGGW 4 cut(s) 156, 249, 267, 711
BsaXI ACNNNNNCTCC 4 cut(s) 1175, 1196, 1205, 1226
Bse118I RCCGGY 1 cut(s) 299
Bse1I ACTGG 3 cut(s) 224, 1039, 1079
BseBI CCWGG 2 cut(s) 51, 235
BseDI CCNNGG 6 cut(s) 234, 261, 349, 555, 565, 836
BseGI GGATG 2 cut(s) 191, 1168
BseMII CTCAG 2 cut(s) 220, 473
BseNI ACTGG 3 cut(s) 224, 1039, 1079
BseXI GCAGC 3 cut(s) 517, 727, 959
BseYI CCCAGC 1 cut(s) 6
Bsh1236I CGCG 2 cut(s) 1153, 1196
Bsh1285I CGRYCG 1 cut(s) 161
BshFI GGCC 5 cut(s) 39, 134, 847, 1127, 1295
BshNI GGYRCC 4 cut(s) 302, 320, 691, 1263
BsiEI CGRYCG 1 cut(s) 161
BsiHKAI GWGCWC 2 cut(s) 294, 1007
BsiHKCI CYCGRG 1 cut(s) 261
BslFI GGGAC 1 cut(s) 473
BsmAI GTCTC 4 cut(s) 27, 334, 702, 1074
BsmBI CGTCTC 2 cut(s) 334, 1074
BsmFI GGGAC 1 cut(s) 473
BsmI GAATGC 1 cut(s) 631
BsnI GGCC 5 cut(s) 39, 134, 847, 1127, 1295
Bso31I GGTCTC 1 cut(s) 702
BsoBI CYCGRG 1 cut(s) 261
Bsp1286I GDGCHC 2 cut(s) 294, 1007
Bsp143I GATC 5 cut(s) 138, 187, 718, 1087, 1228
Bsp1720I GCTNAGC 1 cut(s) 482
Bsp19I CCATGG 1 cut(s) 555
BspANI GGCC 5 cut(s) 39, 134, 847, 1127, 1295
BspCNI CTCAG 2 cut(s) 219, 474
BspFNI CGCG 2 cut(s) 1153, 1196
BspLI GGNNCC 9 cut(s) 40, 229, 248, 266, 304, 322, 693, 1032, 1265
BspMAI CTGCAG 1 cut(s) 1236
BspMI ACCTGC 1 cut(s) 649
BspOI GCTAGC 1 cut(s) 660
BspPI GGATC 2 cut(s) 182, 1095
BspQI GCTCTTC 2 cut(s) 152, 995
BspT107I GGYRCC 4 cut(s) 302, 320, 691, 1263
BspTNI GGTCTC 1 cut(s) 702
BsrBI CCGCTC 1 cut(s) 201
BsrFI RCCGGY 1 cut(s) 299
BsrI ACTGG 3 cut(s) 224, 1039, 1079
BssAI RCCGGY 1 cut(s) 299
BssECI CCNNGG 6 cut(s) 234, 261, 349, 555, 565, 836
BssMI GATC 5 cut(s) 138, 187, 718, 1087, 1228
BssNI GRCGYC 2 cut(s) 303, 980
BssSI CACGAG 1 cut(s) 871
BssT1I CCWWGG 2 cut(s) 555, 836
Bst2BI CACGAG 1 cut(s) 871
Bst2UI CCWGG 2 cut(s) 51, 235
Bst4CI ACNGT 2 cut(s) 826, 931
Bst6I CTCTTC 4 cut(s) 152, 401, 756, 995
BstACI GRCGYC 2 cut(s) 303, 980
BstC8I GCNNGC 5 cut(s) 420, 488, 658, 726, 1300
BstDEI CTNAG 4 cut(s) 206, 482, 687, 747
BstDSI CCRYGG 1 cut(s) 555
BstF5I GGATG 2 cut(s) 191, 1168
BstFNI CGCG 2 cut(s) 1153, 1196
BstH2I RGCGCY 3 cut(s) 306, 349, 425
BstHHI GCGC 5 cut(s) 305, 348, 424, 435, 1198
BstKTI GATC 5 cut(s) 141, 190, 721, 1090, 1231
BstMAI GTCTC 4 cut(s) 27, 334, 702, 1074
BstMBI GATC 5 cut(s) 138, 187, 718, 1087, 1228
BstMCI CGRYCG 1 cut(s) 161
BstMWI GCNNNNNNNGC 5 cut(s) 419, 430, 487, 536, 1204
BstNI CCWGG 2 cut(s) 51, 235
BstNSI RCATGY 1 cut(s) 286
BstSCI CCNGG 9 cut(s) 49, 233, 260, 261, 308, 348, 565, 806, 1007
BstSFI CTRYAG 1 cut(s) 1232
BstUI CGCG 2 cut(s) 1153, 1196
BstV1I GCAGC 3 cut(s) 517, 727, 959
BstV2I GAAGAC 2 cut(s) 118, 899
BstX2I RGATCY 1 cut(s) 1087
BstYI RGATCY 1 cut(s) 1087
BsuRI GGCC 5 cut(s) 39, 134, 847, 1127, 1295
BtgI CCRYGG 1 cut(s) 555
BtrI CACGTC 1 cut(s) 328
BtsCI GGATG 2 cut(s) 191, 1168
BtsI GCAGTG 2 cut(s) 442, 990
BtsIMutI CAGTG 3 cut(s) 442, 927, 990
BveI ACCTGC 1 cut(s) 649
Cac8I GCNNGC 5 cut(s) 420, 488, 658, 726, 1300
CfoI GCGC 5 cut(s) 305, 348, 424, 435, 1198
Cfr10I RCCGGY 1 cut(s) 299
Cfr9I CCCGGG 1 cut(s) 261
CseI GACGC 1 cut(s) 969
Csp6I GTAC 2 cut(s) 692, 1264
CviAII CATG 6 cut(s) 283, 515, 520, 556, 853, 1178
CviQI GTAC 2 cut(s) 692, 1264
DdeI CTNAG 4 cut(s) 206, 482, 687, 747
DinI GGCGCC 1 cut(s) 304
DpnI GATC 5 cut(s) 140, 189, 720, 1089, 1230
DpnII GATC 5 cut(s) 138, 187, 718, 1087, 1228
DraIII CACNNNGTG 1 cut(s) 114
Eam1104I CTCTTC 4 cut(s) 152, 401, 756, 995
EarI CTCTTC 4 cut(s) 152, 401, 756, 995
EciI GGCGGA 3 cut(s) 240, 247, 322
Eco130I CCWWGG 2 cut(s) 555, 836
Eco147I AGGCCT 1 cut(s) 847
Eco31I GGTCTC 1 cut(s) 702
Eco47I GGWCC 8 cut(s) 117, 227, 247, 252, 265, 270, 833, 1031
Eco88I CYCGRG 1 cut(s) 261
EcoO109I RGGNCCY 1 cut(s) 1031
EcoRII CCWGG 2 cut(s) 49, 233
EcoT14I CCWWGG 2 cut(s) 555, 836
EcoT22I ATGCAT 1 cut(s) 525
EgeI GGCGCC 1 cut(s) 304
EheI GGCGCC 1 cut(s) 304
ErhI CCWWGG 2 cut(s) 555, 836
Esp3I CGTCTC 2 cut(s) 334, 1074
FaeI CATG 6 cut(s) 286, 518, 523, 559, 856, 1181
FalI AAGNNNNNCTT 4 cut(s) 680, 712, 959, 991
FaqI GGGAC 1 cut(s) 473
FatI CATG 6 cut(s) 282, 514, 519, 555, 852, 1177
FauI CCCGC 2 cut(s) 203, 238
FauNDI CATATG 1 cut(s) 574
FbaI TGATCA 1 cut(s) 718
Fnu4HI GCNGC 9 cut(s) 199, 306, 336, 531, 741, 915, 973, 1151, 1296
FokI GGATG 2 cut(s) 178, 1175
Fsp4HI GCNGC 9 cut(s) 199, 306, 336, 531, 741, 915, 973, 1151, 1296
FspBI CTAG 4 cut(s) 657, 663, 729, 936
GlaI GCGC 5 cut(s) 304, 347, 423, 434, 1197
GluI GCNGC 9 cut(s) 199, 306, 336, 531, 741, 915, 973, 1151, 1296
GsaI CCCAGC 1 cut(s) 10
GsuI CTGGAG 1 cut(s) 33
HaeII RGCGCY 3 cut(s) 306, 349, 425
HaeIII GGCC 5 cut(s) 39, 134, 847, 1127, 1295
HgaI GACGC 1 cut(s) 969
HhaI GCGC 5 cut(s) 305, 348, 424, 435, 1198
Hin1I GRCGYC 2 cut(s) 303, 980
Hin1II CATG 6 cut(s) 286, 518, 523, 559, 856, 1181
Hin6I GCGC 5 cut(s) 303, 346, 422, 433, 1196
HinP1I GCGC 5 cut(s) 303, 346, 422, 433, 1196
HindIII AAGCTT 1 cut(s) 488
HinfI GANTC 2 cut(s) 78, 470
HphI GGTGA 3 cut(s) 289, 770, 1087
Hpy166II GTNNAC 2 cut(s) 510, 1173
Hpy188I TCNGA 6 cut(s) 83, 187, 209, 703, 903, 1087
Hpy188III TCNNGA 3 cut(s) 29, 273, 871
Hpy8I GTNNAC 2 cut(s) 510, 1173
Hpy99I CGWCG 5 cut(s) 169, 320, 359, 403, 985
HpyAV CCTTC 6 cut(s) 130, 449, 880, 1021, 1111, 1285
HpyCH4III ACNGT 2 cut(s) 826, 931
HpyCH4IV ACGT 2 cut(s) 327, 1067
HpyCH4V TGCA 5 cut(s) 523, 629, 770, 1181, 1234
HpyF10VI GCNNNNNNNGC 5 cut(s) 419, 430, 487, 536, 1204
HpyF3I CTNAG 4 cut(s) 206, 482, 687, 747
HpySE526I ACGT 2 cut(s) 327, 1067
Hsp92I GRCGYC 2 cut(s) 303, 980
Hsp92II CATG 6 cut(s) 286, 518, 523, 559, 856, 1181
HspAI GCGC 5 cut(s) 303, 346, 422, 433, 1196
KasI GGCGCC 1 cut(s) 302
KpnI GGTACC 2 cut(s) 695, 1267
Ksp22I TGATCA 1 cut(s) 718
Kzo9I GATC 5 cut(s) 138, 187, 718, 1087, 1228
LguI GCTCTTC 2 cut(s) 152, 995
LmnI GCTCC 5 cut(s) 98, 206, 430, 1196, 1204
Lsp1109I GCAGC 3 cut(s) 517, 727, 959
MaeI CTAG 4 cut(s) 657, 663, 729, 936
MaeII ACGT 2 cut(s) 327, 1067
MaeIII GTNAC 2 cut(s) 295, 1093
MalI GATC 5 cut(s) 140, 189, 720, 1089, 1230
MbiI CCGCTC 1 cut(s) 201
MboI GATC 5 cut(s) 138, 187, 718, 1087, 1228
MflI RGATCY 1 cut(s) 1087
MhlI GDGCHC 2 cut(s) 294, 1007
MluCI AATT 3 cut(s) 172, 1054, 1250
Mly113I GGCGCC 1 cut(s) 303
MlyI GAGTC 2 cut(s) 87, 464
MmeI TCCRAC 1 cut(s) 966
Mph1103I ATGCAT 1 cut(s) 525
MroXI GAANNNNTTC 1 cut(s) 1250
MseI TTAA 2 cut(s) 603, 681
MslI CAYNNNNRTG 2 cut(s) 287, 789
MspR9I CCNGG 9 cut(s) 51, 235, 262, 263, 310, 350, 567, 808, 1009
Mva1269I GAATGC 1 cut(s) 631
MvaI CCWGG 2 cut(s) 51, 235
MvnI CGCG 2 cut(s) 1153, 1196
MwoI GCNNNNNNNGC 5 cut(s) 419, 430, 487, 536, 1204
NarI GGCGCC 1 cut(s) 303
NciI CCSGG 7 cut(s) 262, 263, 310, 350, 567, 808, 1009
NcoI CCATGG 1 cut(s) 555
NdeI CATATG 1 cut(s) 574
NdeII GATC 5 cut(s) 138, 187, 718, 1087, 1228
NheI GCTAGC 1 cut(s) 656
NlaIII CATG 6 cut(s) 286, 518, 523, 559, 856, 1181
NlaIV GGNNCC 9 cut(s) 40, 229, 248, 266, 304, 322, 693, 1032, 1265
NmeAIII GCCGAG 1 cut(s) 452
NmuCI GTSAC 2 cut(s) 295, 1093
NsiI ATGCAT 1 cut(s) 525
NspI RCATGY 1 cut(s) 286
PceI AGGCCT 1 cut(s) 847
PciSI GCTCTTC 2 cut(s) 152, 995
PcsI WCGNNNNNNNCGW 1 cut(s) 324
PctI GAATGC 1 cut(s) 631
PdmI GAANNNNTTC 1 cut(s) 1250
PkrI GCNGC 9 cut(s) 200, 307, 337, 532, 742, 916, 974, 1152, 1297
PleI GAGTC 2 cut(s) 86, 464
PluTI GGCGCC 1 cut(s) 306
PpsI GAGTC 2 cut(s) 86, 464
PpuMI RGGWCCY 1 cut(s) 1031
PsiI TTATAA 1 cut(s) 780
Psp5II RGGWCCY 1 cut(s) 1031
Psp6I CCWGG 2 cut(s) 49, 233
PspFI CCCAGC 1 cut(s) 6
PspGI CCWGG 2 cut(s) 49, 233
PspN4I GGNNCC 9 cut(s) 40, 229, 248, 266, 304, 322, 693, 1032, 1265
PspPPI RGGWCCY 1 cut(s) 1031
PstI CTGCAG 1 cut(s) 1236
PsuI RGATCY 1 cut(s) 1087
RsaI GTAC 2 cut(s) 693, 1265
RsaNI GTAC 2 cut(s) 692, 1264
RseI CAYNNNNRTG 2 cut(s) 287, 789
SapI GCTCTTC 2 cut(s) 152, 995
SaqAI TTAA 2 cut(s) 603, 681
SatI GCNGC 9 cut(s) 199, 306, 336, 531, 741, 915, 973, 1151, 1296
Sau3AI GATC 5 cut(s) 138, 187, 718, 1087, 1228
SchI GAGTC 2 cut(s) 87, 464
ScrFI CCNGG 9 cut(s) 51, 235, 262, 263, 310, 350, 567, 808, 1009
SduI GDGCHC 2 cut(s) 294, 1007
SfcI CTRYAG 1 cut(s) 1232
SfoI GGCGCC 1 cut(s) 304
SgrAI CRCCGGYG 1 cut(s) 299
SinI GGWCC 8 cut(s) 117, 227, 247, 252, 265, 270, 833, 1031
SmaI CCCGGG 1 cut(s) 263
SmiMI CAYNNNNRTG 2 cut(s) 287, 789
SmlI CTYRAG 3 cut(s) 11, 341, 534
SmoI CTYRAG 3 cut(s) 11, 341, 534
Sse9I AATT 3 cut(s) 172, 1054, 1250
SseBI AGGCCT 1 cut(s) 847
SspDI GGCGCC 1 cut(s) 302
SspMI CTAG 4 cut(s) 657, 663, 729, 936
StuI AGGCCT 1 cut(s) 847
StyD4I CCNGG 9 cut(s) 49, 233, 260, 261, 308, 348, 565, 806, 1007
StyI CCWWGG 2 cut(s) 555, 836
TaaI ACNGT 2 cut(s) 826, 931
TaiI ACGT 2 cut(s) 330, 1070
TasI AATT 3 cut(s) 172, 1054, 1250
TauI GCSGC 6 cut(s) 201, 308, 338, 917, 1153, 1298
Tru1I TTAA 2 cut(s) 603, 681
Tru9I TTAA 2 cut(s) 603, 681
TscAI CASTG 3 cut(s) 442, 934, 997
TseFI GTSAC 2 cut(s) 295, 1093
TseI GCWGC 3 cut(s) 530, 740, 972
Tsp45I GTSAC 2 cut(s) 295, 1093
TspDTI ATGAA 3 cut(s) 436, 561, 569
TspMI CCCGGG 1 cut(s) 261
TspRI CASTG 3 cut(s) 442, 934, 997
VpaK11BI GGWCC 8 cut(s) 117, 227, 247, 252, 265, 270, 833, 1031
XapI RAATTY 1 cut(s) 1250
XceI RCATGY 1 cut(s) 286
XmaI CCCGGG 1 cut(s) 261
XmnI GAANNNNTTC 1 cut(s) 1250
XspI CTAG 4 cut(s) 657, 663, 729, 936
Zsp2I ATGCAT 1 cut(s) 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.