AT4G01130

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0009202
Physical Location & Seq
Forward (+)
485743 .. 488226
2484 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G01130.2

Sequence Viewer

Length: 1149 bp
ATGGCTTCCGATATAAACAGAAGAAGATCGTTCTCTCTTCTAGTCTTGATTATTGTGATGCTTTATGGGCATAAAGGTGATTCAAAATGTGATTTCGAAGCAATTTTCAACTTCGGAGACTCGAATTCGGACACTGGCGGATTTTGGGCTGCTTTTCCTGCCCAATCTGGTCCATGGGGAATGACTTATTTCAAGAAACCAGCCGGTCGTGCTTCAGACGGTCGTCTTATCATTGATTTTCTAGCCAAATCTCTTGGAATGCCTTTCCTAAGCCCATATTTGCAATCTATTGGATCGGATTTTCGACATGGCGCAAACTTTGCGACGTTAGCGTCCACGGTTCTCTTGCCAAACACGTCTTTATTTGTCTCCGGAATCAGCCCTTTCTCACTCGCTATTCAGCTGAATCAGATGAAACAATTCAAGGTTAATGTCGACGAGTCACATTCTTTGGACCGACCAGGATTGAAGATCTTACCGTCTAAAATTGTTTTCGGAAAATCGCTATACACGTTTTATATTGGTCAAAACGATTTCACATCCAATTTAGCCAGTATTGGAGTTGAGAGAGTGAAACTATATCTTCCACAAGTCATAGGCCAAATTGCTGGAACGATTAAGGAGATATATGGAATAGGTGGTCGGACATTTTTGGTATTGAATTTAGCACCGGTTGGATGTTACCCGGCGATTTTAACCGGTTACACTCATACCGATGCTGATTTAGACAAGTATGGATGTCTCATTCCTGTAAACAAAGCAGTCAAATATTACAACACGTTATTGAATAAGACATTATCTCAGACCAGAACCGAACTAAAAAACGCTACCGTAATTTATTTAGACACGCACAAGATATTGCTTGATCTCTTTCAACACCCCAAATCCTACGGTATGAAACACGGTATTAAAGCTTGTTGTGGATATGGTGGACGTCCATACAATTTCAATCAGAAGTTATTTTGTGGCAACACCAAAGTGATTGGAAATTTTTCTACGACGGCCAAAGCTTGCCATGATCCTCACAACTACGTGAGTTGGGATGGAATTCATGCGACGGAGGCCGCAAATCACCACATTTCCATGGCCATTCTCGACGGTTCGATATCATATCCTCCGTTCATACTCAATAACCTATGTTCGCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

382

Amino Acids

42.01

Weight (kDa)

9.09

Isoelectric Point (pI)

34.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 35 - 285 1.2e-35 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 331
AatII GACGTC 1 cut(s) 937
AccI GTMKAC 1 cut(s) 435
AccIII TCCGGA 1 cut(s) 371
AciI CCGC 2 cut(s) 138, 1065
AclWI GGATC 2 cut(s) 301, 1013
AcoI YGGCCR 2 cut(s) 1002, 1086
AcsI RAATTY 4 cut(s) 124, 661, 988, 1047
AcuI CTGAAG 1 cut(s) 198
AcyI GRCGYC 1 cut(s) 934
AflIII ACRYGT 3 cut(s) 354, 510, 777
AgeI ACCGGT 2 cut(s) 670, 698
AgsI TTSAA 9 cut(s) 84, 109, 193, 424, 469, 661, 787, 875, 949
AjiI CACGTC 1 cut(s) 357
AjnI CCWGG 1 cut(s) 460
AleI CACNNNNGTG 1 cut(s) 977
AluBI AGCT 3 cut(s) 403, 914, 1010
AluI AGCT 3 cut(s) 403, 914, 1010
Alw26I GTCTC 3 cut(s) 111, 373, 746
AlwI GGATC 2 cut(s) 301, 1013
Aor13HI TCCGGA 1 cut(s) 371
AoxI GGCC 4 cut(s) 598, 1002, 1062, 1086
ApeKI GCWGC 1 cut(s) 149
ApoI RAATTY 4 cut(s) 124, 661, 988, 1047
AsiGI ACCGGT 2 cut(s) 670, 698
Asp700I GAANNNNTTC 2 cut(s) 419, 991
AspLEI GCGC 1 cut(s) 314
AspS9I GGNCC 2 cut(s) 170, 454
AsuC2I CCSGG 1 cut(s) 686
AsuHPI GGTGA 2 cut(s) 89, 1064
AsuII TTCGAA 1 cut(s) 96
AvaII GGWCC 2 cut(s) 170, 454
BalI TGGCCA 1 cut(s) 1088
BbvI GCAGC 1 cut(s) 136
BccI CCATC 1 cut(s) 1037
BceAI ACGGC 1 cut(s) 1017
BciT130I CCWGG 1 cut(s) 462
BcnI CCSGG 1 cut(s) 686
BcoDI GTCTC 3 cut(s) 111, 373, 746
BfaI CTAG 2 cut(s) 41, 242
BglII AGATCT 1 cut(s) 471
BisI GCNGC 2 cut(s) 150, 1065
BlsI GCNGC 2 cut(s) 151, 1066
Bme1390I CCNGG 2 cut(s) 462, 686
Bme18I GGWCC 2 cut(s) 170, 454
BmgBI CACGTC 1 cut(s) 357
BmgT120I GGNCC 2 cut(s) 170, 454
BmrFI CCNGG 2 cut(s) 462, 686
BmsI GCATC 2 cut(s) 48, 706
BoxI GACNNNNGTC 1 cut(s) 222
Bpu10I CCTNAGC 1 cut(s) 269
Bpu14I TTCGAA 1 cut(s) 96
BpuMI CCSGG 1 cut(s) 686
BsaAI YACGTR 1 cut(s) 1033
BsaHI GRCGYC 1 cut(s) 934
BsaJI CCNNGG 3 cut(s) 173, 336, 1083
BsaWI WCCGGW 3 cut(s) 371, 670, 698
Bse118I RCCGGY 3 cut(s) 203, 670, 698
Bse1I ACTGG 2 cut(s) 139, 552
BseAI TCCGGA 1 cut(s) 371
BseBI CCWGG 1 cut(s) 462
BseDI CCNNGG 3 cut(s) 173, 336, 1083
BseGI GGATG 4 cut(s) 539, 683, 743, 1048
BseMII CTCAG 1 cut(s) 815
BseNI ACTGG 2 cut(s) 139, 552
BseXI GCAGC 1 cut(s) 136
Bsh1285I CGRYCG 2 cut(s) 208, 223
BshFI GGCC 4 cut(s) 600, 1004, 1064, 1088
BshTI ACCGGT 2 cut(s) 670, 698
BsiEI CGRYCG 2 cut(s) 208, 223
BsiSI CCGG 5 cut(s) 204, 372, 671, 686, 699
BsmAI GTCTC 3 cut(s) 111, 373, 746
BsmI GAATGC 1 cut(s) 264
BsnI GGCC 4 cut(s) 600, 1004, 1064, 1088
Bsp119I TTCGAA 1 cut(s) 96
Bsp13I TCCGGA 1 cut(s) 371
Bsp143I GATC 5 cut(s) 26, 293, 471, 865, 1018
Bsp19I CCATGG 2 cut(s) 173, 1083
BspACI CCGC 2 cut(s) 138, 1065
BspANI GGCC 4 cut(s) 600, 1004, 1064, 1088
BspCNI CTCAG 1 cut(s) 814
BspEI TCCGGA 1 cut(s) 371
BspPI GGATC 2 cut(s) 301, 1013
BspT104I TTCGAA 1 cut(s) 96
BsrFI RCCGGY 3 cut(s) 203, 670, 698
BsrI ACTGG 2 cut(s) 139, 552
BssAI RCCGGY 3 cut(s) 203, 670, 698
BssECI CCNNGG 3 cut(s) 173, 336, 1083
BssMI GATC 5 cut(s) 26, 293, 471, 865, 1018
BssNI GRCGYC 1 cut(s) 934
BssT1I CCWWGG 2 cut(s) 173, 1083
Bst2UI CCWGG 1 cut(s) 462
Bst4CI ACNGT 7 cut(s) 221, 340, 480, 832, 893, 905, 1100
Bst6I CTCTTC 1 cut(s) 42
BstACI GRCGYC 1 cut(s) 934
BstAPI GCANNNNNTGC 1 cut(s) 320
BstBAI YACGTR 1 cut(s) 1033
BstBI TTCGAA 1 cut(s) 96
BstC8I GCNNGC 1 cut(s) 1012
BstDEI CTNAG 3 cut(s) 269, 801, 1146
BstDSI CCRYGG 3 cut(s) 173, 336, 1083
BstF5I GGATG 4 cut(s) 539, 683, 743, 1048
BstHHI GCGC 1 cut(s) 314
BstKTI GATC 5 cut(s) 29, 296, 474, 868, 1021
BstMAI GTCTC 3 cut(s) 111, 373, 746
BstMBI GATC 5 cut(s) 26, 293, 471, 865, 1018
BstMCI CGRYCG 2 cut(s) 208, 223
BstMWI GCNNNNNNNGC 6 cut(s) 67, 158, 209, 320, 329, 1061
BstNI CCWGG 1 cut(s) 462
BstPAI GACNNNNGTC 1 cut(s) 222
BstSCI CCNGG 2 cut(s) 460, 684
BstV1I GCAGC 1 cut(s) 136
BstX2I RGATCY 1 cut(s) 471
BstXI CCANNNNNNTGG 1 cut(s) 608
BstYI RGATCY 1 cut(s) 471
BsuRI GGCC 4 cut(s) 600, 1004, 1064, 1088
BtgI CCRYGG 3 cut(s) 173, 336, 1083
BtrI CACGTC 1 cut(s) 357
BtsCI GGATG 4 cut(s) 539, 683, 743, 1048
BtsIMutI CAGTG 1 cut(s) 132
Cac8I GCNNGC 1 cut(s) 1012
CfoI GCGC 1 cut(s) 314
Cfr10I RCCGGY 3 cut(s) 203, 670, 698
Cfr13I GGNCC 2 cut(s) 170, 454
CseI GACGC 1 cut(s) 321
CspAI ACCGGT 2 cut(s) 670, 698
CviAII CATG 5 cut(s) 174, 308, 1016, 1052, 1084
DdeI CTNAG 3 cut(s) 269, 801, 1146
DpnI GATC 5 cut(s) 28, 295, 473, 867, 1020
DpnII GATC 5 cut(s) 26, 293, 471, 865, 1018
DrdI GACNNNNNNGTC 1 cut(s) 331
DseDI GACNNNNNNGTC 1 cut(s) 331
EaeI YGGCCR 2 cut(s) 1002, 1086
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
EciI GGCGGA 1 cut(s) 153
Eco130I CCWWGG 2 cut(s) 173, 1083
Eco32I GATATC 1 cut(s) 1107
Eco47I GGWCC 2 cut(s) 170, 454
Eco57I CTGAAG 1 cut(s) 198
EcoRI GAATTC 2 cut(s) 124, 1047
EcoRII CCWGG 1 cut(s) 460
EcoRV GATATC 1 cut(s) 1107
EcoT14I CCWWGG 2 cut(s) 173, 1083
ErhI CCWWGG 2 cut(s) 173, 1083
FaeI CATG 5 cut(s) 177, 311, 1019, 1055, 1087
FatI CATG 5 cut(s) 173, 307, 1015, 1051, 1083
FblI GTMKAC 1 cut(s) 435
Fnu4HI GCNGC 2 cut(s) 150, 1065
FokI GGATG 4 cut(s) 526, 690, 750, 1055
Fsp4HI GCNGC 2 cut(s) 150, 1065
FspBI CTAG 2 cut(s) 41, 242
GlaI GCGC 1 cut(s) 313
GluI GCNGC 2 cut(s) 150, 1065
HaeIII GGCC 4 cut(s) 600, 1004, 1064, 1088
HapII CCGG 5 cut(s) 204, 372, 671, 686, 699
HgaI GACGC 1 cut(s) 321
HhaI GCGC 1 cut(s) 314
Hin1I GRCGYC 1 cut(s) 934
Hin1II CATG 5 cut(s) 177, 311, 1019, 1055, 1087
Hin6I GCGC 1 cut(s) 312
HinP1I GCGC 1 cut(s) 312
HincII GTYRAC 1 cut(s) 436
HindII GTYRAC 1 cut(s) 436
HindIII AAGCTT 2 cut(s) 912, 1008
HinfI GANTC 5 cut(s) 80, 119, 375, 406, 440
HpaII CCGG 5 cut(s) 204, 372, 671, 686, 699
HphI GGTGA 2 cut(s) 89, 1064
Hpy166II GTNNAC 4 cut(s) 336, 436, 754, 932
Hpy188III TCNNGA 4 cut(s) 46, 193, 372, 1094
Hpy8I GTNNAC 4 cut(s) 336, 436, 754, 932
Hpy99I CGWCG 5 cut(s) 328, 440, 1003, 1060, 1100
HpyCH4III ACNGT 7 cut(s) 221, 340, 480, 832, 893, 905, 1100
HpyCH4IV ACGT 6 cut(s) 326, 356, 512, 779, 934, 1032
HpyCH4V TGCA 1 cut(s) 283
HpyF10VI GCNNNNNNNGC 6 cut(s) 67, 158, 209, 320, 329, 1061
HpyF3I CTNAG 3 cut(s) 269, 801, 1146
HpySE526I ACGT 6 cut(s) 326, 356, 512, 779, 934, 1032
Hsp92I GRCGYC 1 cut(s) 934
Hsp92II CATG 5 cut(s) 177, 311, 1019, 1055, 1087
HspAI GCGC 1 cut(s) 312
Kpn2I TCCGGA 1 cut(s) 371
Kzo9I GATC 5 cut(s) 26, 293, 471, 865, 1018
Lsp1109I GCAGC 1 cut(s) 136
LweI GCATC 2 cut(s) 48, 706
MaeI CTAG 2 cut(s) 41, 242
MaeII ACGT 6 cut(s) 326, 356, 512, 779, 934, 1032
MaeIII GTNAC 3 cut(s) 441, 680, 701
MalI GATC 5 cut(s) 28, 295, 473, 867, 1020
MboI GATC 5 cut(s) 26, 293, 471, 865, 1018
MboII GAAGA 5 cut(s) 29, 33, 36, 481, 575
MflI RGATCY 1 cut(s) 471
MlsI TGGCCA 1 cut(s) 1088
MluNI TGGCCA 1 cut(s) 1088
MlyI GAGTC 2 cut(s) 113, 449
MmeI TCCRAC 2 cut(s) 623, 655
MnlI CCTC 3 cut(s) 1032, 1054, 1125
Mox20I TGGCCA 1 cut(s) 1088
MroI TCCGGA 1 cut(s) 371
MroXI GAANNNNTTC 2 cut(s) 419, 991
MscI TGGCCA 1 cut(s) 1088
MseI TTAA 4 cut(s) 429, 618, 695, 909
MslI CAYNNNNRTG 4 cut(s) 75, 714, 977, 1082
Msp20I TGGCCA 1 cut(s) 1088
MspA1I CMGCKG 1 cut(s) 403
MspI CCGG 5 cut(s) 204, 372, 671, 686, 699
MspR9I CCNGG 2 cut(s) 462, 686
Mva1269I GAATGC 1 cut(s) 264
MvaI CCWGG 1 cut(s) 462
MwoI GCNNNNNNNGC 6 cut(s) 67, 158, 209, 320, 329, 1061
NciI CCSGG 1 cut(s) 686
NcoI CCATGG 2 cut(s) 173, 1083
NdeII GATC 5 cut(s) 26, 293, 471, 865, 1018
NlaIII CATG 5 cut(s) 177, 311, 1019, 1055, 1087
NmuCI GTSAC 1 cut(s) 441
NspV TTCGAA 1 cut(s) 96
OliI CACNNNNGTG 1 cut(s) 977
PcsI WCGNNNNNNNCGW 1 cut(s) 509
PctI GAATGC 1 cut(s) 264
PdmI GAANNNNTTC 2 cut(s) 419, 991
PfeI GAWTC 3 cut(s) 80, 375, 406
PinAI ACCGGT 2 cut(s) 670, 698
PkrI GCNGC 2 cut(s) 151, 1066
PleI GAGTC 2 cut(s) 113, 448
PpsI GAGTC 2 cut(s) 113, 448
Ppu21I YACGTR 1 cut(s) 1033
PshAI GACNNNNGTC 1 cut(s) 222
Psp6I CCWGG 1 cut(s) 460
PspGI CCWGG 1 cut(s) 460
PspPI GGNCC 2 cut(s) 170, 454
PsuI RGATCY 1 cut(s) 471
PvuII CAGCTG 1 cut(s) 403
RseI CAYNNNNRTG 4 cut(s) 75, 714, 977, 1082
SalI GTCGAC 1 cut(s) 434
SaqAI TTAA 4 cut(s) 429, 618, 695, 909
SatI GCNGC 2 cut(s) 150, 1065
Sau3AI GATC 5 cut(s) 26, 293, 471, 865, 1018
Sau96I GGNCC 2 cut(s) 170, 454
SchI GAGTC 2 cut(s) 113, 449
ScrFI CCNGG 2 cut(s) 462, 686
SfaNI GCATC 2 cut(s) 48, 706
SfuI TTCGAA 1 cut(s) 96
SinI GGWCC 2 cut(s) 170, 454
SmiMI CAYNNNNRTG 4 cut(s) 75, 714, 977, 1082
SsiI CCGC 2 cut(s) 138, 1065
SspI AATATT 1 cut(s) 770
SspMI CTAG 2 cut(s) 41, 242
StyD4I CCNGG 2 cut(s) 460, 684
StyI CCWWGG 2 cut(s) 173, 1083
TaaI ACNGT 7 cut(s) 221, 340, 480, 832, 893, 905, 1100
TaiI ACGT 6 cut(s) 329, 359, 515, 782, 937, 1035
TaqI TCGA 6 cut(s) 96, 122, 304, 435, 1095, 1103
TaqII GACCGA 1 cut(s) 471
TauI GCSGC 1 cut(s) 1067
TfiI GAWTC 3 cut(s) 80, 375, 406
Tru1I TTAA 4 cut(s) 429, 618, 695, 909
Tru9I TTAA 4 cut(s) 429, 618, 695, 909
TscAI CASTG 1 cut(s) 139
TseFI GTSAC 1 cut(s) 441
TseI GCWGC 1 cut(s) 149
Tsp45I GTSAC 1 cut(s) 441
TspDTI ATGAA 4 cut(s) 428, 911, 1040, 1111
TspGWI ACGGA 2 cut(s) 1073, 1107
TspRI CASTG 1 cut(s) 139
VpaK11BI GGWCC 2 cut(s) 170, 454
XapI RAATTY 4 cut(s) 124, 661, 988, 1047
XmiI GTMKAC 1 cut(s) 435
XmnI GAANNNNTTC 2 cut(s) 419, 991
XspI CTAG 2 cut(s) 41, 242
ZraI GACGTC 1 cut(s) 935
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.