MD07G1143700.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009202
Physical Location & Seq
Reverse (-)
20949412 .. 20952614
3203 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1143700.v1.1.491

Sequence Viewer

Length: 1179 bp
ATGAGCCTGTGTGTACCAAAAATTTGTGTACTCAACAAGATTTTAATCGTCTGGATGACAGTGGTCGCAACTTTGAGTTGTTTAGGTCATTCTAAAAGTGAATTCGAGGCAATTTTCAATTTTGAAAATTCGAACTCAGATACAGGCAGATTTTGGGCAGCTTTTCTGGCGCAGCCTGGACCCTATGGCATGACATACTTCAAGAGACCTGTTGGTCGGGCTACGGATGGGAGGCTCATTGTTGATTTTCTAGGTAACTCACACAATCTTCAGTTTGGTCAAAATTACTTAACGGTAGATATGCTTCCTGATTCTTTGCTTTTGTTGAACTTGGCAGCTCAAGCTCTAGGGTTACCATTTATAAGTCCATATCTGAAATCAATTGGATCTGATTATAAACATTGGGCAAACTATGCAACACTAGCATCCATTGTGCTTTTGCCAAACACTTCATTGTTTGTCACTGGAATCAGCCCATTCTCTCTGGCCATCAATGAAGAGACTTCCTTCCCTGGATATTTTTGGGAAATCAGTCTACACATTCTAAACGATTTCACCTCCAATTTGGCAGCCATCAGTGTAGGTGGAGTGAAGCAATATCTTCCTCTGGTCTCCCAAATTGCTGGTACCATCAAGGAGCTTTATACATTAGGAGGGCGTGCATTTCTGGTTCTTAATCTGGCATCAGTGGGTTGTTATCCATCATTTTTGGCGGGGCTTCCTCCTTCAGAACTCGACGCATTTGGATGCTCAATTGCTTACAACAAAGCGGTTGTGGACTACAACAACTTGTTGAAGGAGGCACTTAGCCAAACTAGAGGCTCCCTCCCAAATGCTTCCCTCGTATCCCTCGTATATGTGGACACCTCCTCTGTTTTGCTTGAGCTCTTTAGGCATCCCACTTCCCACGGTTTGAAGTATGGTACAAAATCATGTTGTGGGCATGGTGGTGGTACCTATAATTTTGACCCCCAAGTTTTTTGTGGAAATACAAAGGTGATCAATGGGAAACCTGTGACTGCAACTGCTTGTAGTGACCCTTACAACTATGTGAGCTGGGATGGAGTCCATGCCACAGAAGCTGCAAACAAGCTAACGACATTGGCTATCCTCAACGACACTTTCTCAGACCCTCCCTTTCCCCTTGAGCAGTTCTGTGACCTCCACGGTATTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

393

Amino Acids

42.49

Weight (kDa)

5.82

Isoelectric Point (pI)

28.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 183 - 366 4.3e-14 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 362, 396
AasI GACNNNNNNGTC 1 cut(s) 213
Acc65I GGTACC 2 cut(s) 626, 953
AccB1I GGYRCC 2 cut(s) 626, 953
AccI GTMKAC 1 cut(s) 535
AciI CCGC 2 cut(s) 713, 770
AclWI GGATC 1 cut(s) 394
AcoI YGGCCR 1 cut(s) 486
AcsI RAATTY 3 cut(s) 21, 101, 127
AcuI CTGAAG 2 cut(s) 254, 711
AfaI GTAC 5 cut(s) 15, 30, 628, 925, 955
AgsI TTSAA 6 cut(s) 118, 125, 202, 328, 796, 916
AjnI CCWGG 2 cut(s) 175, 511
AluBI AGCT 8 cut(s) 161, 338, 344, 640, 886, 1056, 1082, 1093
AluI AGCT 8 cut(s) 161, 338, 344, 640, 886, 1056, 1082, 1093
Alw21I GWGCWC 1 cut(s) 888
Alw26I GTCTC 3 cut(s) 199, 494, 616
AlwI GGATC 1 cut(s) 394
AlwNI CAGNNNCTG 1 cut(s) 1082
AoxI GGCC 1 cut(s) 486
ApeKI GCWGC 5 cut(s) 158, 172, 335, 569, 1082
ApoI RAATTY 3 cut(s) 21, 101, 127
Asp718I GGTACC 2 cut(s) 626, 953
AspLEI GCGC 1 cut(s) 172
AspS9I GGNCC 1 cut(s) 179
AsuHPI GGTGA 2 cut(s) 547, 1009
AsuII TTCGAA 1 cut(s) 131
AvaII GGWCC 1 cut(s) 179
BalI TGGCCA 1 cut(s) 488
BanI GGYRCC 2 cut(s) 626, 953
BanII GRGCYC 1 cut(s) 888
BarI GAAGNNNNNNTAC 2 cut(s) 288, 320
Bbv12I GWGCWC 1 cut(s) 888
BbvI GCAGC 5 cut(s) 170, 184, 347, 581, 1069
BccI CCATC 6 cut(s) 221, 497, 581, 638, 709, 1055
BciT130I CCWGG 2 cut(s) 177, 513
BciVI GTATCC 1 cut(s) 856
BclI TGATCA 1 cut(s) 999
BcoDI GTCTC 3 cut(s) 199, 494, 616
BfaI CTAG 4 cut(s) 251, 347, 422, 816
BfuI GTATCC 1 cut(s) 856
BisI GCNGC 5 cut(s) 159, 173, 336, 570, 1083
BlsI GCNGC 5 cut(s) 160, 174, 337, 571, 1084
Bme1390I CCNGG 2 cut(s) 177, 513
Bme18I GGWCC 1 cut(s) 179
BmgT120I GGNCC 1 cut(s) 179
BmiI GGNNCC 4 cut(s) 181, 628, 823, 955
BmrFI CCNGG 2 cut(s) 177, 513
BmsI GCATC 4 cut(s) 434, 692, 737, 904
BoxI GACNNNNGTC 1 cut(s) 62
BplI GAGNNNNNCTC 2 cut(s) 810, 842
Bpu14I TTCGAA 1 cut(s) 131
BpuEI CTTGAG 3 cut(s) 324, 902, 1166
BsaBI GATNNNNATC 1 cut(s) 44
BsaI GGTCTC 2 cut(s) 199, 616
BsaJI CCNNGG 3 cut(s) 511, 907, 1165
Bse1I ACTGG 1 cut(s) 469
Bse8I GATNNNNATC 1 cut(s) 44
BseBI CCWGG 2 cut(s) 177, 513
BseDI CCNNGG 3 cut(s) 511, 907, 1165
BseGI GGATG 6 cut(s) 60, 232, 425, 752, 895, 1066
BseJI GATNNNNATC 1 cut(s) 44
BseMII CTCAG 2 cut(s) 150, 1140
BseNI ACTGG 1 cut(s) 469
BseRI GAGGAG 1 cut(s) 859
BseXI GCAGC 5 cut(s) 170, 184, 347, 581, 1069
BseYI CCCAGC 1 cut(s) 1056
BshFI GGCC 1 cut(s) 488
BshNI GGYRCC 2 cut(s) 626, 953
BsiHKAI GWGCWC 1 cut(s) 888
BsmAI GTCTC 3 cut(s) 199, 494, 616
BsnI GGCC 1 cut(s) 488
Bso31I GGTCTC 2 cut(s) 199, 616
Bsp119I TTCGAA 1 cut(s) 131
Bsp1286I GDGCHC 1 cut(s) 888
Bsp143I GATC 2 cut(s) 386, 999
BspACI CCGC 2 cut(s) 713, 770
BspANI GGCC 1 cut(s) 488
BspCNI CTCAG 2 cut(s) 149, 1139
BspLI GGNNCC 4 cut(s) 181, 628, 823, 955
BspPI GGATC 1 cut(s) 394
BspT104I TTCGAA 1 cut(s) 131
BspT107I GGYRCC 2 cut(s) 626, 953
BspTNI GGTCTC 2 cut(s) 199, 616
BsrI ACTGG 1 cut(s) 469
BssECI CCNNGG 3 cut(s) 511, 907, 1165
BssMI GATC 2 cut(s) 386, 999
Bst2UI CCWGG 2 cut(s) 177, 513
Bst4CI ACNGT 4 cut(s) 61, 295, 911, 1169
Bst6I CTCTTC 1 cut(s) 492
BstAPI GCANNNNNTGC 1 cut(s) 413
BstBI TTCGAA 1 cut(s) 131
BstC8I GCNNGC 1 cut(s) 660
BstDEI CTNAG 3 cut(s) 136, 806, 1126
BstDSI CCRYGG 2 cut(s) 907, 1165
BstEII GGTNACC 1 cut(s) 351
BstF5I GGATG 6 cut(s) 60, 232, 425, 752, 895, 1066
BstHHI GCGC 1 cut(s) 172
BstKTI GATC 2 cut(s) 389, 1002
BstMAI GTCTC 3 cut(s) 199, 494, 616
BstMBI GATC 2 cut(s) 386, 999
BstMWI GCNNNNNNNGC 6 cut(s) 167, 341, 413, 422, 892, 1079
BstNI CCWGG 2 cut(s) 177, 513
BstPAI GACNNNNGTC 1 cut(s) 62
BstPI GGTNACC 1 cut(s) 351
BstSCI CCNGG 2 cut(s) 175, 511
BstV1I GCAGC 5 cut(s) 170, 184, 347, 581, 1069
BstX2I RGATCY 1 cut(s) 386
BstXI CCANNNNNNTGG 1 cut(s) 623
BstYI RGATCY 1 cut(s) 386
BsuI GTATCC 1 cut(s) 856
BsuRI GGCC 1 cut(s) 488
BtgI CCRYGG 2 cut(s) 907, 1165
BtsCI GGATG 6 cut(s) 60, 232, 425, 752, 895, 1066
BtsIMutI CAGTG 4 cut(s) 66, 462, 583, 693
Cac8I GCNNGC 1 cut(s) 660
CaiI CAGNNNCTG 1 cut(s) 1082
CfoI GCGC 1 cut(s) 172
Cfr13I GGNCC 1 cut(s) 179
CseI GACGC 1 cut(s) 746
Csp6I GTAC 5 cut(s) 14, 29, 627, 924, 954
CviAII CATG 4 cut(s) 190, 933, 944, 1070
CviQI GTAC 5 cut(s) 14, 29, 627, 924, 954
DdeI CTNAG 3 cut(s) 136, 806, 1126
DpnI GATC 2 cut(s) 388, 1001
DpnII GATC 2 cut(s) 386, 999
DrdI GACNNNNNNGTC 1 cut(s) 213
DseDI GACNNNNNNGTC 1 cut(s) 213
EaeI YGGCCR 1 cut(s) 486
Eam1104I CTCTTC 1 cut(s) 492
EarI CTCTTC 1 cut(s) 492
Ecl136II GAGCTC 1 cut(s) 886
Eco24I GRGCYC 1 cut(s) 888
Eco31I GGTCTC 2 cut(s) 199, 616
Eco47I GGWCC 1 cut(s) 179
Eco53kI GAGCTC 1 cut(s) 886
Eco57I CTGAAG 2 cut(s) 254, 711
Eco91I GGTNACC 1 cut(s) 351
EcoICRI GAGCTC 1 cut(s) 886
EcoO65I GGTNACC 1 cut(s) 351
EcoRI GAATTC 1 cut(s) 101
EcoRII CCWGG 2 cut(s) 175, 511
EcoT38I GRGCYC 1 cut(s) 888
FaeI CATG 4 cut(s) 193, 936, 947, 1073
FatI CATG 4 cut(s) 189, 932, 943, 1069
FauI CCCGC 1 cut(s) 706
FbaI TGATCA 1 cut(s) 999
FblI GTMKAC 1 cut(s) 535
Fnu4HI GCNGC 5 cut(s) 159, 173, 336, 570, 1083
FokI GGATG 6 cut(s) 67, 239, 412, 759, 882, 1073
FriOI GRGCYC 1 cut(s) 888
Fsp4HI GCNGC 5 cut(s) 159, 173, 336, 570, 1083
FspBI CTAG 4 cut(s) 251, 347, 422, 816
GlaI GCGC 1 cut(s) 171
GluI GCNGC 5 cut(s) 159, 173, 336, 570, 1083
GsaI CCCAGC 1 cut(s) 1060
HaeIII GGCC 1 cut(s) 488
HgaI GACGC 1 cut(s) 746
HhaI GCGC 1 cut(s) 172
Hin1II CATG 4 cut(s) 193, 936, 947, 1073
Hin6I GCGC 1 cut(s) 170
HinP1I GCGC 1 cut(s) 170
HinfI GANTC 3 cut(s) 311, 468, 1065
HphI GGTGA 2 cut(s) 547, 1009
Hpy166II GTNNAC 5 cut(s) 14, 29, 536, 778, 862
Hpy188I TCNGA 5 cut(s) 139, 375, 391, 730, 1129
Hpy188III TCNNGA 3 cut(s) 52, 202, 308
Hpy8I GTNNAC 5 cut(s) 14, 29, 536, 778, 862
Hpy99I CGWCG 1 cut(s) 740
HpyAV CCTTC 3 cut(s) 517, 735, 790
HpyCH4III ACNGT 4 cut(s) 61, 295, 911, 1169
HpyCH4V TGCA 4 cut(s) 416, 662, 1022, 1085
HpyF10VI GCNNNNNNNGC 6 cut(s) 167, 341, 413, 422, 892, 1079
HpyF3I CTNAG 3 cut(s) 136, 806, 1126
Hsp92II CATG 4 cut(s) 193, 936, 947, 1073
HspAI GCGC 1 cut(s) 170
KpnI GGTACC 2 cut(s) 630, 957
Ksp22I TGATCA 1 cut(s) 999
Kzo9I GATC 2 cut(s) 386, 999
LmnI GCTCC 2 cut(s) 637, 827
Lsp1109I GCAGC 5 cut(s) 170, 184, 347, 581, 1069
LweI GCATC 4 cut(s) 434, 692, 737, 904
MaeI CTAG 4 cut(s) 251, 347, 422, 816
MaeIII GTNAC 6 cut(s) 254, 351, 460, 1015, 1034, 1157
MalI GATC 2 cut(s) 388, 1001
MboI GATC 2 cut(s) 386, 999
MboII GAAGA 3 cut(s) 260, 509, 593
MfeI CAATTG 2 cut(s) 381, 753
MflI RGATCY 1 cut(s) 386
MhlI GDGCHC 1 cut(s) 888
MlsI TGGCCA 1 cut(s) 488
MluNI TGGCCA 1 cut(s) 488
MlyI GAGTC 1 cut(s) 1074
Mox20I TGGCCA 1 cut(s) 488
MscI TGGCCA 1 cut(s) 488
MseI TTAA 3 cut(s) 44, 290, 675
MslI CAYNNNNRTG 2 cut(s) 745, 948
Msp20I TGGCCA 1 cut(s) 488
MspR9I CCNGG 2 cut(s) 177, 513
MunI CAATTG 2 cut(s) 381, 753
MvaI CCWGG 2 cut(s) 177, 513
MwoI GCNNNNNNNGC 6 cut(s) 167, 341, 413, 422, 892, 1079
NdeII GATC 2 cut(s) 386, 999
NlaIII CATG 4 cut(s) 193, 936, 947, 1073
NlaIV GGNNCC 4 cut(s) 181, 628, 823, 955
NmuCI GTSAC 4 cut(s) 460, 1015, 1034, 1157
NspV TTCGAA 1 cut(s) 131
PcsI WCGNNNNNNNCGW 1 cut(s) 849
PfeI GAWTC 2 cut(s) 311, 468
PkrI GCNGC 5 cut(s) 160, 174, 337, 571, 1084
PleI GAGTC 1 cut(s) 1073
PpsI GAGTC 1 cut(s) 1073
PshAI GACNNNNGTC 1 cut(s) 62
PsiI TTATAA 2 cut(s) 362, 396
Psp124BI GAGCTC 1 cut(s) 888
Psp6I CCWGG 2 cut(s) 175, 511
PspEI GGTNACC 1 cut(s) 351
PspFI CCCAGC 1 cut(s) 1056
PspGI CCWGG 2 cut(s) 175, 511
PspN4I GGNNCC 4 cut(s) 181, 628, 823, 955
PspPI GGNCC 1 cut(s) 179
PstNI CAGNNNCTG 1 cut(s) 1082
PsuI RGATCY 1 cut(s) 386
RsaI GTAC 5 cut(s) 15, 30, 628, 925, 955
RsaNI GTAC 5 cut(s) 14, 29, 627, 924, 954
RseI CAYNNNNRTG 2 cut(s) 745, 948
SacI GAGCTC 1 cut(s) 888
SaqAI TTAA 3 cut(s) 44, 290, 675
SatI GCNGC 5 cut(s) 159, 173, 336, 570, 1083
Sau3AI GATC 2 cut(s) 386, 999
Sau96I GGNCC 1 cut(s) 179
SchI GAGTC 1 cut(s) 1074
ScrFI CCNGG 2 cut(s) 177, 513
SduI GDGCHC 1 cut(s) 888
SfaNI GCATC 4 cut(s) 434, 692, 737, 904
SfuI TTCGAA 1 cut(s) 131
SinI GGWCC 1 cut(s) 179
SmiMI CAYNNNNRTG 2 cut(s) 745, 948
SmlI CTYRAG 3 cut(s) 339, 881, 1145
SmoI CTYRAG 3 cut(s) 339, 881, 1145
SsiI CCGC 2 cut(s) 713, 770
SspMI CTAG 4 cut(s) 251, 347, 422, 816
SstI GAGCTC 1 cut(s) 888
StyD4I CCNGG 2 cut(s) 175, 511
TaaI ACNGT 4 cut(s) 61, 295, 911, 1169
TaqI TCGA 3 cut(s) 105, 131, 735
TatI WGTACW 1 cut(s) 28
TfiI GAWTC 2 cut(s) 311, 468
Tru1I TTAA 3 cut(s) 44, 290, 675
Tru9I TTAA 3 cut(s) 44, 290, 675
TscAI CASTG 4 cut(s) 66, 469, 583, 693
TseFI GTSAC 4 cut(s) 460, 1015, 1034, 1157
TseI GCWGC 5 cut(s) 158, 172, 335, 569, 1082
Tsp45I GTSAC 4 cut(s) 460, 1015, 1034, 1157
TspDTI ATGAA 2 cut(s) 441, 510
TspGWI ACGGA 1 cut(s) 239
TspRI CASTG 4 cut(s) 66, 469, 583, 693
VpaK11BI GGWCC 1 cut(s) 179
XapI RAATTY 3 cut(s) 21, 101, 127
XcmI CCANNNNNNNNNTGG 1 cut(s) 980
XmiI GTMKAC 1 cut(s) 535
XspI CTAG 4 cut(s) 251, 347, 422, 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.