MD07G1143600.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009202
Physical Location & Seq
Reverse (-)
20927427 .. 20930380
2954 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1143600.v1.1.491

Sequence Viewer

Length: 1197 bp
ATGCCAGTCATTCTATCTGTTACTGAGAACATGAGCCTCTGTGTACCAAAAATTTGTGTCCTCCACAAGATTTTAATCGTATGGATGACAGTGGTGGCAACTTTGAGTTGGTTATGTTACTCTAAATGTGAATTCGAGGCAATTTTCAATTTCGGAGATTCAAACTCAGATACAGGTGGTTTTTGGGCAGCTTTTCCGGCGCAATCTGGACCCTATGGCATGACATACTTCAAGAGACCTGTTGGTCGGGCTACAGATGGGAGGCTCATTGTTGATTTTCTAGCTCAAGCTCTAGGGTTACCATTTATAAGTCCATATCTGAAATCAATTGGATCTGATTATAAACATGGGGCAAACTATGCAACACTAGCATCCACTGTGCTTTTGCCAAACACTTCATTGTTTGTCACTGGAATCAGCCCATTCTCTCTGGCCATCCAGCTCAACCAAATGAAGGCATTCAAGACCCAAGTTGAAGAGTTTCACCACTCCTCGAATCAACAGGAACTGAAGAGACTTCCTTCCCTGGATATTTTTGGGAAATCACTCTACACATTCTACATTGGCCAAAACGATTTCACCTCCAATTTGGCAGCCATTGGTGTAGGTGGAGTGAAGCAATATCTTCCTCAAGTGGTCTCCCAAATTGCTGTTACCATCAAGGAGCTATATGCATTAGGAGGGCGTGCATTTCTGGTACTTAATCTGGCACCAGTGGGTTGTTATCCATCATTTTTGGCGGGGCTTCCTCCTTCTGAACTCGACGCATTTGGATGTTCAATTGCTTACAACAAAGCAGTTGTGGACTACAACAAGTTGTTGAAGGAGGCACTTAGCCAAACTAGAGGCTCCCTCTCAAATGCTTCCCTCGTATATGTGGACACCTCCTCTGTTTTGCTTGAGCTCTTTAGGCATCCCACATCTCATGGCTTGAAGTATGGTACAAAATCATGTTGTGGGCATGGTGGTGGTGCCCATAATTTTGACCCTCAAGTTTTTTGTGGAAATACAAAGGTGATCAATGGGAGACCTGTGACTGCAACTGCTTGTAGTGACCCTTACAACTATGTGAGCTGGGATGGAGTCCATGCCACAGAAGCTGCAAACAAGCTAACAACATTGGCTATCCTCAACGGCACTTTCTCTGACCCTCCCCTTCCCTTTGAGCAGTTCTGTGACCTCCACCGTATTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

399

Amino Acids

43.22

Weight (kDa)

7.55

Isoelectric Point (pI)

33.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 48 - 372 5.5e-41 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 308, 342
AasI GACNNNNNNGTC 1 cut(s) 243
AccB1I GGYRCC 2 cut(s) 709, 971
AciI CCGC 1 cut(s) 740
AclWI GGATC 1 cut(s) 340
AcoI YGGCCR 2 cut(s) 432, 565
AcsI RAATTY 2 cut(s) 51, 131
AcuI CTGAAG 1 cut(s) 530
AfaI GTAC 3 cut(s) 45, 699, 943
AfiI CCNNNNNNNGG 1 cut(s) 454
AgsI TTSAA 8 cut(s) 148, 162, 232, 463, 476, 780, 823, 934
AjnI CCWGG 1 cut(s) 525
AluBI AGCT 9 cut(s) 191, 284, 290, 442, 667, 904, 1074, 1100, 1111
AluI AGCT 9 cut(s) 191, 284, 290, 442, 667, 904, 1074, 1100, 1111
Alw21I GWGCWC 1 cut(s) 906
Alw26I GTCTC 4 cut(s) 229, 508, 643, 1021
AlwI GGATC 1 cut(s) 340
AlwNI CAGNNNCTG 2 cut(s) 508, 1100
AoxI GGCC 2 cut(s) 432, 565
ApeKI GCWGC 3 cut(s) 188, 593, 1100
ApoI RAATTY 2 cut(s) 51, 131
Asp700I GAANNNNTTC 2 cut(s) 458, 480
AspLEI GCGC 1 cut(s) 202
AspS9I GGNCC 1 cut(s) 209
AsuHPI GGTGA 3 cut(s) 476, 571, 1027
AvaII GGWCC 1 cut(s) 209
BaeGI GKGCMC 1 cut(s) 976
BalI TGGCCA 2 cut(s) 434, 567
BanI GGYRCC 2 cut(s) 709, 971
BanII GRGCYC 1 cut(s) 906
Bbv12I GWGCWC 1 cut(s) 906
BbvI GCAGC 3 cut(s) 200, 605, 1087
BccI CCATC 5 cut(s) 251, 443, 665, 736, 1073
BceAI ACGGC 1 cut(s) 1150
BciT130I CCWGG 1 cut(s) 527
BclI TGATCA 1 cut(s) 1017
BcoDI GTCTC 4 cut(s) 229, 508, 643, 1021
BfaI CTAG 4 cut(s) 281, 293, 368, 843
BfmI CTRYAG 1 cut(s) 252
BisI GCNGC 3 cut(s) 189, 594, 1101
BlsI GCNGC 3 cut(s) 190, 595, 1102
Bme1390I CCNGG 1 cut(s) 527
Bme18I GGWCC 1 cut(s) 209
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 4 cut(s) 211, 711, 850, 973
BmrFI CCNGG 1 cut(s) 527
BmsI GCATC 2 cut(s) 380, 922
BplI GAGNNNNNCTC 2 cut(s) 837, 869
BpuEI CTTGAG 4 cut(s) 270, 615, 920, 975
BsaBI GATNNNNATC 1 cut(s) 74
BsaI GGTCTC 3 cut(s) 229, 643, 1021
BsaJI CCNNGG 1 cut(s) 525
Bsc4I CCNNNNNNNGG 1 cut(s) 454
Bse1I ACTGG 3 cut(s) 5, 415, 713
Bse8I GATNNNNATC 1 cut(s) 74
BseBI CCWGG 1 cut(s) 527
BseDI CCNNGG 1 cut(s) 525
BseGI GGATG 6 cut(s) 90, 371, 435, 779, 913, 1084
BseJI GATNNNNATC 1 cut(s) 74
BseLI CCNNNNNNNGG 1 cut(s) 454
BseMII CTCAG 2 cut(s) 15, 180
BseNI ACTGG 3 cut(s) 5, 415, 713
BseRI GAGGAG 2 cut(s) 481, 877
BseSI GKGCMC 1 cut(s) 976
BseXI GCAGC 3 cut(s) 200, 605, 1087
BseYI CCCAGC 1 cut(s) 1074
BshFI GGCC 2 cut(s) 434, 567
BshNI GGYRCC 2 cut(s) 709, 971
BsiHKAI GWGCWC 1 cut(s) 906
BsiSI CCGG 1 cut(s) 197
BslI CCNNNNNNNGG 1 cut(s) 454
BsmAI GTCTC 4 cut(s) 229, 508, 643, 1021
BsmI GAATGC 1 cut(s) 458
BsnI GGCC 2 cut(s) 434, 567
Bso31I GGTCTC 3 cut(s) 229, 643, 1021
Bsp1286I GDGCHC 2 cut(s) 906, 976
Bsp143I GATC 2 cut(s) 332, 1017
BspACI CCGC 1 cut(s) 740
BspANI GGCC 2 cut(s) 434, 567
BspCNI CTCAG 2 cut(s) 16, 179
BspLI GGNNCC 4 cut(s) 211, 711, 850, 973
BspPI GGATC 1 cut(s) 340
BspT107I GGYRCC 2 cut(s) 709, 971
BspTNI GGTCTC 3 cut(s) 229, 643, 1021
BsrI ACTGG 3 cut(s) 5, 415, 713
BssECI CCNNGG 1 cut(s) 525
BssMI GATC 2 cut(s) 332, 1017
Bst2UI CCWGG 1 cut(s) 527
Bst4CI ACNGT 3 cut(s) 91, 379, 1187
Bst6I CTCTTC 2 cut(s) 471, 506
BstAPI GCANNNNNTGC 1 cut(s) 359
BstC8I GCNNGC 1 cut(s) 687
BstDEI CTNAG 3 cut(s) 24, 166, 833
BstEII GGTNACC 1 cut(s) 297
BstF5I GGATG 6 cut(s) 90, 371, 435, 779, 913, 1084
BstHHI GCGC 1 cut(s) 202
BstKTI GATC 2 cut(s) 335, 1020
BstMAI GTCTC 4 cut(s) 229, 508, 643, 1021
BstMBI GATC 2 cut(s) 332, 1017
BstMWI GCNNNNNNNGC 5 cut(s) 197, 359, 368, 910, 1097
BstNI CCWGG 1 cut(s) 527
BstPI GGTNACC 1 cut(s) 297
BstSCI CCNGG 1 cut(s) 525
BstSFI CTRYAG 1 cut(s) 252
BstSLI GKGCMC 1 cut(s) 976
BstV1I GCAGC 3 cut(s) 200, 605, 1087
BstX2I RGATCY 1 cut(s) 332
BstYI RGATCY 1 cut(s) 332
BsuRI GGCC 2 cut(s) 434, 567
BtsCI GGATG 6 cut(s) 90, 371, 435, 779, 913, 1084
BtsIMutI CAGTG 4 cut(s) 96, 375, 408, 720
Cac8I GCNNGC 1 cut(s) 687
CaiI CAGNNNCTG 2 cut(s) 508, 1100
CfoI GCGC 1 cut(s) 202
Cfr13I GGNCC 1 cut(s) 209
CseI GACGC 1 cut(s) 773
Csp6I GTAC 3 cut(s) 44, 698, 942
CviAII CATG 7 cut(s) 31, 220, 347, 926, 951, 962, 1088
CviQI GTAC 3 cut(s) 44, 698, 942
DdeI CTNAG 3 cut(s) 24, 166, 833
DpnI GATC 2 cut(s) 334, 1019
DpnII GATC 2 cut(s) 332, 1017
DrdI GACNNNNNNGTC 1 cut(s) 243
DseDI GACNNNNNNGTC 1 cut(s) 243
EaeI YGGCCR 2 cut(s) 432, 565
Eam1104I CTCTTC 2 cut(s) 471, 506
EarI CTCTTC 2 cut(s) 471, 506
Ecl136II GAGCTC 1 cut(s) 904
Eco24I GRGCYC 1 cut(s) 906
Eco31I GGTCTC 3 cut(s) 229, 643, 1021
Eco47I GGWCC 1 cut(s) 209
Eco53kI GAGCTC 1 cut(s) 904
Eco57I CTGAAG 1 cut(s) 530
Eco91I GGTNACC 1 cut(s) 297
EcoICRI GAGCTC 1 cut(s) 904
EcoO65I GGTNACC 1 cut(s) 297
EcoRI GAATTC 1 cut(s) 131
EcoRII CCWGG 1 cut(s) 525
EcoT22I ATGCAT 1 cut(s) 676
EcoT38I GRGCYC 1 cut(s) 906
FaeI CATG 7 cut(s) 34, 223, 350, 929, 954, 965, 1091
FatI CATG 7 cut(s) 30, 219, 346, 925, 950, 961, 1087
FauI CCCGC 1 cut(s) 733
FbaI TGATCA 1 cut(s) 1017
Fnu4HI GCNGC 3 cut(s) 189, 594, 1101
FokI GGATG 6 cut(s) 97, 358, 422, 786, 900, 1091
FriOI GRGCYC 1 cut(s) 906
Fsp4HI GCNGC 3 cut(s) 189, 594, 1101
FspBI CTAG 4 cut(s) 281, 293, 368, 843
GlaI GCGC 1 cut(s) 201
GluI GCNGC 3 cut(s) 189, 594, 1101
GsaI CCCAGC 1 cut(s) 1078
HaeIII GGCC 2 cut(s) 434, 567
HapII CCGG 1 cut(s) 197
HgaI GACGC 1 cut(s) 773
HhaI GCGC 1 cut(s) 202
Hin1II CATG 7 cut(s) 34, 223, 350, 929, 954, 965, 1091
Hin6I GCGC 1 cut(s) 200
HinP1I GCGC 1 cut(s) 200
HinfI GANTC 4 cut(s) 158, 414, 496, 1083
HpaII CCGG 1 cut(s) 197
HphI GGTGA 3 cut(s) 476, 571, 1027
Hpy166II GTNNAC 3 cut(s) 44, 805, 880
Hpy188I TCNGA 6 cut(s) 155, 169, 321, 337, 757, 1147
Hpy188III TCNNGA 3 cut(s) 207, 232, 463
Hpy8I GTNNAC 3 cut(s) 44, 805, 880
Hpy99I CGWCG 1 cut(s) 767
HpyAV CCTTC 5 cut(s) 448, 531, 762, 817, 1166
HpyCH4III ACNGT 3 cut(s) 91, 379, 1187
HpyCH4V TGCA 5 cut(s) 362, 674, 689, 1040, 1103
HpyF10VI GCNNNNNNNGC 5 cut(s) 197, 359, 368, 910, 1097
HpyF3I CTNAG 3 cut(s) 24, 166, 833
Hsp92II CATG 7 cut(s) 34, 223, 350, 929, 954, 965, 1091
HspAI GCGC 1 cut(s) 200
Ksp22I TGATCA 1 cut(s) 1017
Kzo9I GATC 2 cut(s) 332, 1017
LmnI GCTCC 2 cut(s) 664, 854
Lsp1109I GCAGC 3 cut(s) 200, 605, 1087
LweI GCATC 2 cut(s) 380, 922
MaeI CTAG 4 cut(s) 281, 293, 368, 843
MaeIII GTNAC 8 cut(s) 19, 116, 297, 406, 652, 1033, 1052, 1175
MalI GATC 2 cut(s) 334, 1019
MboI GATC 2 cut(s) 332, 1017
MboII GAAGA 3 cut(s) 488, 523, 617
MfeI CAATTG 2 cut(s) 327, 780
MflI RGATCY 1 cut(s) 332
MhlI GDGCHC 2 cut(s) 906, 976
MlsI TGGCCA 2 cut(s) 434, 567
MluCI AATT 9 cut(s) 51, 131, 141, 148, 327, 586, 645, 780, 979
MluNI TGGCCA 2 cut(s) 434, 567
MlyI GAGTC 1 cut(s) 1092
Mox20I TGGCCA 2 cut(s) 434, 567
Mph1103I ATGCAT 1 cut(s) 676
MroXI GAANNNNTTC 2 cut(s) 458, 480
MscI TGGCCA 2 cut(s) 434, 567
MseI TTAA 2 cut(s) 74, 702
MslI CAYNNNNRTG 2 cut(s) 772, 966
Msp20I TGGCCA 2 cut(s) 434, 567
MspI CCGG 1 cut(s) 197
MspR9I CCNGG 1 cut(s) 527
MunI CAATTG 2 cut(s) 327, 780
Mva1269I GAATGC 1 cut(s) 458
MvaI CCWGG 1 cut(s) 527
MwoI GCNNNNNNNGC 5 cut(s) 197, 359, 368, 910, 1097
NdeII GATC 2 cut(s) 332, 1017
NlaIII CATG 7 cut(s) 34, 223, 350, 929, 954, 965, 1091
NlaIV GGNNCC 4 cut(s) 211, 711, 850, 973
NmuCI GTSAC 4 cut(s) 406, 1033, 1052, 1175
NsiI ATGCAT 1 cut(s) 676
PctI GAATGC 1 cut(s) 458
PdmI GAANNNNTTC 2 cut(s) 458, 480
PfeI GAWTC 3 cut(s) 158, 414, 496
PkrI GCNGC 3 cut(s) 190, 595, 1102
PleI GAGTC 1 cut(s) 1091
PpsI GAGTC 1 cut(s) 1091
PsiI TTATAA 2 cut(s) 308, 342
Psp124BI GAGCTC 1 cut(s) 906
Psp6I CCWGG 1 cut(s) 525
PspEI GGTNACC 1 cut(s) 297
PspFI CCCAGC 1 cut(s) 1074
PspGI CCWGG 1 cut(s) 525
PspN4I GGNNCC 4 cut(s) 211, 711, 850, 973
PspPI GGNCC 1 cut(s) 209
PstNI CAGNNNCTG 2 cut(s) 508, 1100
PsuI RGATCY 1 cut(s) 332
RsaI GTAC 3 cut(s) 45, 699, 943
RsaNI GTAC 3 cut(s) 44, 698, 942
RseI CAYNNNNRTG 2 cut(s) 772, 966
SacI GAGCTC 1 cut(s) 906
SaqAI TTAA 2 cut(s) 74, 702
SatI GCNGC 3 cut(s) 189, 594, 1101
Sau3AI GATC 2 cut(s) 332, 1017
Sau96I GGNCC 1 cut(s) 209
SchI GAGTC 1 cut(s) 1092
ScrFI CCNGG 1 cut(s) 527
SduI GDGCHC 2 cut(s) 906, 976
SfaNI GCATC 2 cut(s) 380, 922
SfcI CTRYAG 1 cut(s) 252
SinI GGWCC 1 cut(s) 209
SmiMI CAYNNNNRTG 2 cut(s) 772, 966
SmlI CTYRAG 4 cut(s) 285, 630, 899, 990
SmoI CTYRAG 4 cut(s) 285, 630, 899, 990
Sse9I AATT 9 cut(s) 51, 131, 141, 148, 327, 586, 645, 780, 979
SsiI CCGC 1 cut(s) 740
SspMI CTAG 4 cut(s) 281, 293, 368, 843
SstI GAGCTC 1 cut(s) 906
StyD4I CCNGG 1 cut(s) 525
TaaI ACNGT 3 cut(s) 91, 379, 1187
TaqI TCGA 3 cut(s) 135, 494, 762
TasI AATT 9 cut(s) 51, 131, 141, 148, 327, 586, 645, 780, 979
TfiI GAWTC 3 cut(s) 158, 414, 496
Tru1I TTAA 2 cut(s) 74, 702
Tru9I TTAA 2 cut(s) 74, 702
TscAI CASTG 4 cut(s) 96, 382, 415, 720
TseFI GTSAC 4 cut(s) 406, 1033, 1052, 1175
TseI GCWGC 3 cut(s) 188, 593, 1100
Tsp45I GTSAC 4 cut(s) 406, 1033, 1052, 1175
TspDTI ATGAA 2 cut(s) 387, 467
TspRI CASTG 4 cut(s) 96, 382, 415, 720
VpaK11BI GGWCC 1 cut(s) 209
XapI RAATTY 2 cut(s) 51, 131
XmnI GAANNNNTTC 2 cut(s) 458, 480
XspI CTAG 4 cut(s) 281, 293, 368, 843
Zsp2I ATGCAT 1 cut(s) 676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.