Rorug01G0262700

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001049
Physical Location & Seq
Forward (+)
37258863 .. 37262067
3205 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0262700.1

Sequence Viewer

Length: 708 bp
ATGAGTACATTGGATGCAACTAGAGCCGAACTTGCTCTTGCAGTTCTTTATCTGAACAAGGCTGAGGCCAGGGACAAGATATGCAGGGCGATACAATATGGTTCTAAATTCTTGAGTAATGGACAACCCGGGACAGCCCAAAATGTTGACAAAACGACTAGCTTGGCACGAAAAGTTTTCCGTCTTTTCAAGTTTGTGAATGATCTTCATGGTCTGATTAGTCCAACTGCTCCTGGAACTCCTCTTCCACTTGTTCTGCTTGGAAAGTCCAAAAATGCATTGCTGTCAACTTTTCTGTTTCTTGACCAAATTGTCTGGCTTGGCAGAACAGGCATCTATAAGAACAAAGAACGTGTTGAGCTAATTGGCCGTATATCTCTTTACTGTTGGATGAGTTCCTCAGTTTGCACCACTTTAGTTGAGGTTGGGGAGCTAGGAAGGCTTTCTGGACAAATTAAAAAGTTGGAGAAGGATCTGAAGACCGGCGACAAGTATCAAAATGAGGCGTACCGTGCTAAACTAAAAAAATCGAATGAAAGGTCACTAGCCCTGGTTAAAGCAGCCTTGGATACAGTGGTTGCAATTGGGTTGCTTCAGTTGGCACCTAAGAAAATCACTCCTCGTGTTACTGGAGCCTTGGGATTTACTACTTCTCTGATCTCTTGTTATCAGTTGCTTCCAGCTCCAGTGAAGGCTAAAACATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

235

Amino Acids

25.72

Weight (kDa)

9.9

Isoelectric Point (pI)

21.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEX11 PF05648 13 - 225 7.5e-48 Peroxisomal biogenesis factor 11 (PEX11)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 311
AccB1I GGYRCC 1 cut(s) 601
AclWI GGATC 1 cut(s) 480
AcoI YGGCCR 1 cut(s) 367
AcsI RAATTY 1 cut(s) 107
AcuI CTGAAG 2 cut(s) 497, 578
AfaI GTAC 2 cut(s) 7, 509
AflIII ACRYGT 1 cut(s) 352
AgsI TTSAA 1 cut(s) 190
AjnI CCWGG 3 cut(s) 68, 232, 549
AloI GAACNNNNNNTCC 2 cut(s) 229, 261
AluBI AGCT 4 cut(s) 162, 361, 433, 683
AluI AGCT 4 cut(s) 162, 361, 433, 683
AlwI GGATC 1 cut(s) 480
Ama87I CYCGRG 1 cut(s) 128
AoxI GGCC 2 cut(s) 66, 367
ApeKI GCWGC 1 cut(s) 560
ApoI RAATTY 1 cut(s) 107
ArsI GACNNNNNNTTYG 2 cut(s) 524, 556
Asp700I GAANNNNTTC 1 cut(s) 442
AsuC2I CCSGG 2 cut(s) 129, 130
AvaI CYCGRG 1 cut(s) 128
BaeI ACNNNNGTAYC 2 cut(s) 561, 594
BanI GGYRCC 1 cut(s) 601
BauI CACGAG 1 cut(s) 621
BbsI GAAGAC 1 cut(s) 485
BbvCI CCTCAGC 1 cut(s) 63
BbvI GCAGC 1 cut(s) 572
BceAI ACGGC 1 cut(s) 354
BciT130I CCWGG 3 cut(s) 70, 234, 551
BciVI GTATCC 1 cut(s) 562
BcnI CCSGG 2 cut(s) 129, 130
BfaI CTAG 4 cut(s) 21, 159, 434, 545
BfuI GTATCC 1 cut(s) 562
BisI GCNGC 1 cut(s) 561
BlsI GCNGC 1 cut(s) 562
Bme1390I CCNGG 5 cut(s) 70, 129, 130, 234, 551
BmeT110I CYCGRG 1 cut(s) 128
BmiI GGNNCC 2 cut(s) 603, 634
BmrFI CCNGG 5 cut(s) 70, 129, 130, 234, 551
BmsI GCATC 2 cut(s) 4, 342
BpiI GAAGAC 1 cut(s) 485
BpmI CTGGAG 2 cut(s) 651, 669
Bpu10I CCTNAGC 1 cut(s) 63
BpuEI CTTGAG 1 cut(s) 133
BpuMI CCSGG 2 cut(s) 129, 130
BsaJI CCNNGG 5 cut(s) 69, 128, 549, 564, 636
Bse118I RCCGGY 1 cut(s) 482
Bse1I ACTGG 2 cut(s) 634, 686
Bse3DI GCAATG 1 cut(s) 278
BseBI CCWGG 3 cut(s) 70, 234, 551
BseDI CCNNGG 5 cut(s) 69, 128, 549, 564, 636
BseGI GGATG 3 cut(s) 19, 396, 701
BseMI GCAATG 1 cut(s) 278
BseMII CTCAG 2 cut(s) 54, 414
BseNI ACTGG 2 cut(s) 634, 686
BseRI GAGGAG 2 cut(s) 231, 609
BseXI GCAGC 1 cut(s) 572
BshFI GGCC 2 cut(s) 68, 369
BshNI GGYRCC 1 cut(s) 601
BsiHKCI CYCGRG 1 cut(s) 128
BsiSI CCGG 2 cut(s) 129, 483
BslFI GGGAC 2 cut(s) 86, 145
BsmFI GGGAC 2 cut(s) 86, 145
BsnI GGCC 2 cut(s) 68, 369
BsoBI CYCGRG 1 cut(s) 128
Bsp143I GATC 3 cut(s) 202, 472, 657
BspANI GGCC 2 cut(s) 68, 369
BspCNI CTCAG 2 cut(s) 55, 413
BspLI GGNNCC 2 cut(s) 603, 634
BspPI GGATC 1 cut(s) 480
BspT107I GGYRCC 1 cut(s) 601
BsrDI GCAATG 1 cut(s) 278
BsrFI RCCGGY 1 cut(s) 482
BsrI ACTGG 2 cut(s) 634, 686
BssAI RCCGGY 1 cut(s) 482
BssECI CCNNGG 5 cut(s) 69, 128, 549, 564, 636
BssMI GATC 3 cut(s) 202, 472, 657
BssSI CACGAG 1 cut(s) 621
BssT1I CCWWGG 2 cut(s) 564, 636
Bst2BI CACGAG 1 cut(s) 621
Bst2UI CCWGG 3 cut(s) 70, 234, 551
Bst4CI ACNGT 3 cut(s) 386, 512, 574
Bst6I CTCTTC 1 cut(s) 249
BstDEI CTNAG 3 cut(s) 63, 400, 606
BstF5I GGATG 3 cut(s) 19, 396, 701
BstKTI GATC 3 cut(s) 205, 475, 660
BstMBI GATC 3 cut(s) 202, 472, 657
BstMWI GCNNNNNNNGC 5 cut(s) 23, 32, 330, 439, 512
BstNI CCWGG 3 cut(s) 70, 234, 551
BstSCI CCNGG 5 cut(s) 68, 127, 128, 232, 549
BstV1I GCAGC 1 cut(s) 572
BstV2I GAAGAC 1 cut(s) 485
BstX2I RGATCY 1 cut(s) 472
BstYI RGATCY 1 cut(s) 472
BsuI GTATCC 1 cut(s) 562
BsuRI GGCC 2 cut(s) 68, 369
BtsCI GGATG 3 cut(s) 19, 396, 701
BtsIMutI CAGTG 2 cut(s) 579, 693
Cfr10I RCCGGY 1 cut(s) 482
Cfr9I CCCGGG 1 cut(s) 128
Csp6I GTAC 2 cut(s) 6, 508
CspCI CAANNNNNGTGG 2 cut(s) 400, 435
CviAII CATG 1 cut(s) 209
CviQI GTAC 2 cut(s) 6, 508
DdeI CTNAG 3 cut(s) 63, 400, 606
DpnI GATC 3 cut(s) 204, 474, 659
DpnII GATC 3 cut(s) 202, 472, 657
DrdI GACNNNNNNGTC 1 cut(s) 311
DseDI GACNNNNNNGTC 1 cut(s) 311
EaeI YGGCCR 1 cut(s) 367
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
Eco130I CCWWGG 2 cut(s) 564, 636
Eco57I CTGAAG 2 cut(s) 497, 578
Eco88I CYCGRG 1 cut(s) 128
EcoRII CCWGG 3 cut(s) 68, 232, 549
EcoT14I CCWWGG 2 cut(s) 564, 636
EcoT22I ATGCAT 1 cut(s) 280
ErhI CCWWGG 2 cut(s) 564, 636
FaeI CATG 1 cut(s) 212
FaiI YATR 5 cut(s) 82, 99, 210, 339, 374
FaqI GGGAC 2 cut(s) 86, 145
FatI CATG 1 cut(s) 208
Fnu4HI GCNGC 1 cut(s) 561
FokI GGATG 3 cut(s) 26, 403, 688
Fsp4HI GCNGC 1 cut(s) 561
FspBI CTAG 4 cut(s) 21, 159, 434, 545
GluI GCNGC 1 cut(s) 561
GsuI CTGGAG 2 cut(s) 651, 669
HaeIII GGCC 2 cut(s) 68, 369
HapII CCGG 2 cut(s) 129, 483
Hin1II CATG 1 cut(s) 212
HincII GTYRAC 2 cut(s) 148, 288
HindII GTYRAC 2 cut(s) 148, 288
HpaII CCGG 2 cut(s) 129, 483
Hpy166II GTNNAC 2 cut(s) 148, 288
Hpy188I TCNGA 4 cut(s) 54, 216, 477, 657
Hpy188III TCNNGA 3 cut(s) 112, 302, 447
Hpy8I GTNNAC 2 cut(s) 148, 288
HpyAV CCTTC 3 cut(s) 432, 463, 685
HpyCH4III ACNGT 3 cut(s) 386, 512, 574
HpyCH4IV ACGT 1 cut(s) 352
HpyCH4V TGCA 6 cut(s) 17, 41, 84, 278, 408, 581
HpyF10VI GCNNNNNNNGC 5 cut(s) 23, 32, 330, 439, 512
HpyF3I CTNAG 3 cut(s) 63, 400, 606
HpySE526I ACGT 1 cut(s) 352
Hsp92II CATG 1 cut(s) 212
Kzo9I GATC 3 cut(s) 202, 472, 657
LmnI GCTCC 4 cut(s) 235, 430, 632, 688
Lsp1109I GCAGC 1 cut(s) 572
LweI GCATC 2 cut(s) 4, 342
MaeI CTAG 4 cut(s) 21, 159, 434, 545
MaeII ACGT 1 cut(s) 352
MaeIII GTNAC 2 cut(s) 540, 625
MalI GATC 3 cut(s) 204, 474, 659
MboI GATC 3 cut(s) 202, 472, 657
MboII GAAGA 3 cut(s) 197, 236, 490
MfeI CAATTG 1 cut(s) 582
MflI RGATCY 1 cut(s) 472
MluCI AATT 5 cut(s) 107, 309, 363, 453, 582
MmeI TCCRAC 3 cut(s) 248, 368, 444
MnlI CCTC 6 cut(s) 58, 252, 409, 415, 496, 630
Mph1103I ATGCAT 1 cut(s) 280
MroXI GAANNNNTTC 1 cut(s) 442
MseI TTAA 2 cut(s) 456, 555
MspI CCGG 2 cut(s) 129, 483
MspR9I CCNGG 5 cut(s) 70, 129, 130, 234, 551
MunI CAATTG 1 cut(s) 582
MvaI CCWGG 3 cut(s) 70, 234, 551
MwoI GCNNNNNNNGC 5 cut(s) 23, 32, 330, 439, 512
NciI CCSGG 2 cut(s) 129, 130
NdeII GATC 3 cut(s) 202, 472, 657
NlaIII CATG 1 cut(s) 212
NlaIV GGNNCC 2 cut(s) 603, 634
NmuCI GTSAC 1 cut(s) 540
NsiI ATGCAT 1 cut(s) 280
PdmI GAANNNNTTC 1 cut(s) 442
PfoI TCCNGGA 1 cut(s) 232
PkrI GCNGC 1 cut(s) 562
Psp6I CCWGG 3 cut(s) 68, 232, 549
PspGI CCWGG 3 cut(s) 68, 232, 549
PspN4I GGNNCC 2 cut(s) 603, 634
PsuI RGATCY 1 cut(s) 472
RsaI GTAC 2 cut(s) 7, 509
RsaNI GTAC 2 cut(s) 6, 508
SaqAI TTAA 2 cut(s) 456, 555
SatI GCNGC 1 cut(s) 561
Sau3AI GATC 3 cut(s) 202, 472, 657
ScrFI CCNGG 5 cut(s) 70, 129, 130, 234, 551
SetI ASST 8 cut(s) 164, 355, 363, 426, 435, 542, 607, 685
SfaNI GCATC 2 cut(s) 4, 342
SmaI CCCGGG 1 cut(s) 130
SmlI CTYRAG 1 cut(s) 112
SmoI CTYRAG 1 cut(s) 112
Sse9I AATT 5 cut(s) 107, 309, 363, 453, 582
SspMI CTAG 4 cut(s) 21, 159, 434, 545
StyD4I CCNGG 5 cut(s) 68, 127, 128, 232, 549
StyI CCWWGG 2 cut(s) 564, 636
TaaI ACNGT 3 cut(s) 386, 512, 574
TaiI ACGT 1 cut(s) 355
TaqI TCGA 1 cut(s) 530
TasI AATT 5 cut(s) 107, 309, 363, 453, 582
TatI WGTACW 1 cut(s) 5
Tru1I TTAA 2 cut(s) 456, 555
Tru9I TTAA 2 cut(s) 456, 555
TscAI CASTG 2 cut(s) 579, 693
TseFI GTSAC 1 cut(s) 540
TseI GCWGC 1 cut(s) 560
Tsp45I GTSAC 1 cut(s) 540
TspDTI ATGAA 2 cut(s) 197, 549
TspGWI ACGGA 1 cut(s) 170
TspMI CCCGGG 1 cut(s) 128
TspRI CASTG 2 cut(s) 579, 693
XapI RAATTY 1 cut(s) 107
XmaI CCCGGG 1 cut(s) 128
XmnI GAANNNNTTC 1 cut(s) 442
XspI CTAG 4 cut(s) 21, 159, 434, 545
Zsp2I ATGCAT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.