Rorug02G0219400

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001049
Physical Location & Seq
Reverse (-)
21124979 .. 21130686
5708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0219400.1

Sequence Viewer

Length: 1335 bp
ATGGATGAAGAATACGACGTCATAGTGCTCGGAACTGGCCTCAAAGAATGCATTCTCAGCGGCCTTCTCTCCGTTGATGGTCTCAAGGTACTGCATATGGATAGGAATGACTATTATGGAGGAGAGTCTACTTCACTTAATCTCAACCAGCTTTGGAAGAGATTCAGGGGCGATGATAAGCCTCCAGAGACTTTGGGCTCAAGTAGAGACTACAATGTTGATATGATCCCCAAGTTCATGATGGCAAATGGTGGTTTGGTTCGCGTCCTCATTCACACCGATGTTACTAAATATCTGAATTTCAAGGCTGTAGATGGTAGTTTTGTGTATAACAAAGGGAAGATCCACAAAGTACCAGCTAATGACGTGGAAGCACTCAAATCTCCACTCATGGGATTATTTGAGAAGCGCCGTGCTCGCAAGTTTTTCCTTTATGTGCAAGACTATGAGGAGAATAATCCAAAATCTCATGAAGGCATGGACCTCAGCCAAGTTACAGCAAGAGATCTTATTTCGAAGTACGGGCTAGATGACAACACAGTTGATTTCATTGGGCATGCACTAGCACTCCACCGAGATGACAATTACTTGGGAGAGCCTGCTCTGGATTTTGTGAAGAGGATGAAGTTATATGCAGAGTCCTTAGCACGCTTTCAAGGAGGATCTCCTTATATCTACCCATTATATGGTCTAGGAGAGCTACCCCAGGCATTTGCAAGGCTGAGTGCTGTTTATGGAGGCACATACATGCTCAGCAAGCCAGAGTGCAAGGTAGAGTTTGATGAAAGTGGAAAGGCATTTGGTGTGACTTCAGAAGGCGAAACTGCAAAGTGCAAAAAAGTTGTATGTGATCCTTCTTATTTGCCTGATAAGGTGACGAAGGTGGGGAAAGTTGCCCGTGCTATATGTATTATGAGCCATCCCATCCCAAACACCAACGATTCTCATTCAGTGCAGGTTATTCTGCCTCAGAAGCAACTTGGACGTAAATCTGATATGTATCTTTTTTGCTGCTCCTATTCTCACAATGTAGCTCCAAGTGGGAAATACATTGCTTTTGTTTTAACAGAAGCAGAGACCGATAATCCTCAGGTTGAGTTGAAGCCTGGCACAGATCTCCTGGGACCTATAGATGAGATCTTTTTTGACACTTACGATAGATACAAACCTACTAACCAGTCTGATGTTGACCACTGTTTCATATCTGGGAGTTATGATGCGACTACACACTTTGAGACTACTGTACAAGATGTGCTTGACATGTATAGCAAGATAACTGGAAAGGTTCTTGACTTGACTGTGGATCTGAGTGCTGCCAGCGCCGCTGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Actin

Protein Analysis

444

Amino Acids

49.57

Weight (kDa)

5.33

Isoelectric Point (pI)

28.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDI PF00996 1 - 433 1.9e-226 GDP dissociation inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 21
Acc36I ACCTGC 1 cut(s) 946
AccB7I CCANNNNNTGG 1 cut(s) 686
AccI GTMKAC 1 cut(s) 128
AccII CGCG 1 cut(s) 264
AciI CCGC 2 cut(s) 60, 1323
AclWI GGATC 5 cut(s) 220, 337, 670, 845, 1311
AcsI RAATTY 1 cut(s) 298
AcuI CTGAAG 1 cut(s) 795
AcyI GRCGYC 1 cut(s) 18
AfaI GTAC 4 cut(s) 90, 354, 521, 1245
AfiI CCNNNNNNNGG 2 cut(s) 392, 686
AflIII ACRYGT 1 cut(s) 1260
AgsI TTSAA 3 cut(s) 304, 656, 1102
AjiI CACGTC 1 cut(s) 367
AjnI CCWGG 3 cut(s) 705, 1105, 1119
AluBI AGCT 4 cut(s) 151, 359, 700, 1034
AluI AGCT 4 cut(s) 151, 359, 700, 1034
Alw21I GWGCWC 2 cut(s) 30, 418
Alw26I GTCTC 5 cut(s) 86, 182, 201, 1070, 1229
AlwI GGATC 5 cut(s) 220, 337, 670, 845, 1311
AoxI GGCC 2 cut(s) 37, 61
ApeKI GCWGC 2 cut(s) 1011, 1313
ApoI RAATTY 1 cut(s) 298
Asp700I GAANNNNTTC 2 cut(s) 51, 161
AspLEI GCGC 2 cut(s) 411, 1322
AspS9I GGNCC 2 cut(s) 481, 1124
AsuHPI GGTGA 1 cut(s) 886
AsuII TTCGAA 1 cut(s) 515
AvaII GGWCC 2 cut(s) 481, 1124
AxyI CCTNAGG 1 cut(s) 1089
BanII GRGCYC 1 cut(s) 200
Bbv12I GWGCWC 2 cut(s) 30, 418
BbvCI CCTCAGC 1 cut(s) 485
BbvI GCAGC 2 cut(s) 998, 1300
BccI CCATC 5 cut(s) 71, 235, 308, 927, 932
BceAI ACGGC 1 cut(s) 396
BciT130I CCWGG 3 cut(s) 707, 1107, 1121
BcoDI GTCTC 5 cut(s) 86, 182, 201, 1070, 1229
BfaI CTAG 3 cut(s) 527, 563, 692
BfmI CTRYAG 2 cut(s) 309, 1128
BfoI RGCGCY 2 cut(s) 412, 1323
BfuAI ACCTGC 1 cut(s) 946
BglII AGATCT 3 cut(s) 505, 1114, 1137
BisI GCNGC 4 cut(s) 61, 1012, 1314, 1323
BlpI GCTNAGC 1 cut(s) 752
BlsI GCNGC 4 cut(s) 62, 1013, 1315, 1324
Bme1390I CCNGG 3 cut(s) 707, 1107, 1121
Bme18I GGWCC 2 cut(s) 481, 1124
BmgBI CACGTC 1 cut(s) 367
BmgT120I GGNCC 2 cut(s) 481, 1124
BmiI GGNNCC 1 cut(s) 1125
BmrFI CCNGG 3 cut(s) 707, 1107, 1121
BmsI GCATC 1 cut(s) 1207
BpmI CTGGAG 1 cut(s) 168
Bpu10I CCTNAGC 2 cut(s) 485, 643
Bpu1102I GCTNAGC 1 cut(s) 752
Bpu14I TTCGAA 1 cut(s) 515
BpuEI CTTGAG 2 cut(s) 68, 184
BsaBI GATNNNNATC 1 cut(s) 999
BsaHI GRCGYC 1 cut(s) 18
BsaI GGTCTC 2 cut(s) 86, 1070
BsaJI CCNNGG 2 cut(s) 705, 1120
BsaXI ACNNNNNCTCC 2 cut(s) 552, 582
Bsc4I CCNNNNNNNGG 2 cut(s) 392, 686
Bse1I ACTGG 3 cut(s) 40, 1177, 1282
Bse21I CCTNAGG 1 cut(s) 1089
Bse3DI GCAATG 1 cut(s) 1050
Bse8I GATNNNNATC 1 cut(s) 999
BseBI CCWGG 3 cut(s) 707, 1107, 1121
BseDI CCNNGG 2 cut(s) 705, 1120
BseGI GGATG 4 cut(s) 10, 627, 919, 924
BseJI GATNNNNATC 1 cut(s) 999
BseLI CCNNNNNNNGG 2 cut(s) 392, 686
BseMI GCAATG 1 cut(s) 1050
BseMII CTCAG 7 cut(s) 70, 499, 713, 766, 983, 1103, 1298
BseNI ACTGG 3 cut(s) 40, 1177, 1282
BseRI GAGGAG 2 cut(s) 135, 464
BseXI GCAGC 2 cut(s) 998, 1300
BsgI GTGCAG 1 cut(s) 974
Bsh1236I CGCG 1 cut(s) 264
BshFI GGCC 2 cut(s) 39, 63
BsiHKAI GWGCWC 2 cut(s) 30, 418
BslFI GGGAC 1 cut(s) 1137
BslI CCNNNNNNNGG 2 cut(s) 392, 686
BsmAI GTCTC 5 cut(s) 86, 182, 201, 1070, 1229
BsmFI GGGAC 1 cut(s) 1137
BsmI GAATGC 2 cut(s) 51, 53
BsnI GGCC 2 cut(s) 39, 63
Bso31I GGTCTC 2 cut(s) 86, 1070
Bsp119I TTCGAA 1 cut(s) 515
Bsp1286I GDGCHC 3 cut(s) 30, 200, 418
Bsp1407I TGTACA 1 cut(s) 1243
Bsp143I GATC 8 cut(s) 225, 342, 505, 662, 850, 1114, 1137, 1303
Bsp1720I GCTNAGC 1 cut(s) 752
BspACI CCGC 2 cut(s) 60, 1323
BspANI GGCC 2 cut(s) 39, 63
BspCNI CTCAG 7 cut(s) 69, 498, 714, 765, 982, 1102, 1299
BspFNI CGCG 1 cut(s) 264
BspHI TCATGA 2 cut(s) 237, 469
BspLI GGNNCC 1 cut(s) 1125
BspMI ACCTGC 1 cut(s) 946
BspPI GGATC 5 cut(s) 220, 337, 670, 845, 1311
BspT104I TTCGAA 1 cut(s) 515
BspTNI GGTCTC 2 cut(s) 86, 1070
BsrDI GCAATG 1 cut(s) 1050
BsrGI TGTACA 1 cut(s) 1243
BsrI ACTGG 3 cut(s) 40, 1177, 1282
BssECI CCNNGG 2 cut(s) 705, 1120
BssMI GATC 8 cut(s) 225, 342, 505, 662, 850, 1114, 1137, 1303
BssNI GRCGYC 1 cut(s) 18
Bst2UI CCWGG 3 cut(s) 707, 1107, 1121
Bst4CI ACNGT 4 cut(s) 541, 1196, 1243, 1300
Bst6I CTCTTC 2 cut(s) 152, 611
BstACI GRCGYC 1 cut(s) 18
BstAUI TGTACA 1 cut(s) 1243
BstBI TTCGAA 1 cut(s) 515
BstC8I GCNNGC 6 cut(s) 418, 558, 600, 649, 758, 1318
BstDEI CTNAG 8 cut(s) 56, 485, 643, 722, 752, 969, 1089, 1307
BstF5I GGATG 4 cut(s) 10, 627, 919, 924
BstFNI CGCG 1 cut(s) 264
BstH2I RGCGCY 2 cut(s) 412, 1323
BstHHI GCGC 2 cut(s) 411, 1322
BstKTI GATC 8 cut(s) 228, 345, 508, 665, 853, 1117, 1140, 1306
BstMAI GTCTC 5 cut(s) 86, 182, 201, 1070, 1229
BstMBI GATC 8 cut(s) 225, 342, 505, 662, 850, 1114, 1137, 1303
BstMWI GCNNNNNNNGC 6 cut(s) 57, 417, 757, 973, 1319, 1322
BstNI CCWGG 3 cut(s) 707, 1107, 1121
BstNSI RCATGY 3 cut(s) 560, 751, 1264
BstSCI CCNGG 3 cut(s) 705, 1105, 1119
BstSFI CTRYAG 2 cut(s) 309, 1128
BstUI CGCG 1 cut(s) 264
BstV1I GCAGC 2 cut(s) 998, 1300
BstX2I RGATCY 6 cut(s) 342, 505, 662, 1114, 1137, 1303
BstYI RGATCY 6 cut(s) 342, 505, 662, 1114, 1137, 1303
Bsu36I CCTNAGG 1 cut(s) 1089
BsuRI GGCC 2 cut(s) 39, 63
BtgZI GCGATG 1 cut(s) 186
BtrI CACGTC 1 cut(s) 367
BtsCI GGATG 4 cut(s) 10, 627, 919, 924
BtsIMutI CAGTG 2 cut(s) 957, 1192
BveI ACCTGC 1 cut(s) 946
Cac8I GCNNGC 6 cut(s) 418, 558, 600, 649, 758, 1318
CciI TCATGA 2 cut(s) 237, 469
CfoI GCGC 2 cut(s) 411, 1322
Cfr13I GGNCC 2 cut(s) 481, 1124
CseI GACGC 1 cut(s) 253
Csp6I GTAC 4 cut(s) 89, 353, 520, 1244
CviAII CATG 7 cut(s) 238, 391, 470, 478, 557, 748, 1261
CviQI GTAC 4 cut(s) 89, 353, 520, 1244
DdeI CTNAG 8 cut(s) 56, 485, 643, 722, 752, 969, 1089, 1307
DpnI GATC 8 cut(s) 227, 344, 507, 664, 852, 1116, 1139, 1305
DpnII GATC 8 cut(s) 225, 342, 505, 662, 850, 1114, 1137, 1303
Eam1104I CTCTTC 2 cut(s) 152, 611
EarI CTCTTC 2 cut(s) 152, 611
Eco24I GRGCYC 1 cut(s) 200
Eco31I GGTCTC 2 cut(s) 86, 1070
Eco47I GGWCC 2 cut(s) 481, 1124
Eco57I CTGAAG 1 cut(s) 795
Eco81I CCTNAGG 1 cut(s) 1089
EcoO109I RGGNCCY 1 cut(s) 1124
EcoRII CCWGG 3 cut(s) 705, 1105, 1119
EcoT22I ATGCAT 1 cut(s) 53
EcoT38I GRGCYC 1 cut(s) 200
FaeI CATG 7 cut(s) 241, 394, 473, 481, 560, 751, 1264
FalI AAGNNNNNCTT 2 cut(s) 1239, 1271
FaqI GGGAC 1 cut(s) 1137
FatI CATG 7 cut(s) 237, 390, 469, 477, 556, 747, 1260
FauNDI CATATG 1 cut(s) 96
FblI GTMKAC 1 cut(s) 128
Fnu4HI GCNGC 4 cut(s) 61, 1012, 1314, 1323
FokI GGATG 4 cut(s) 17, 634, 906, 911
FriOI GRGCYC 1 cut(s) 200
Fsp4HI GCNGC 4 cut(s) 61, 1012, 1314, 1323
FspBI CTAG 3 cut(s) 527, 563, 692
GlaI GCGC 2 cut(s) 410, 1321
GluI GCNGC 4 cut(s) 61, 1012, 1314, 1323
GsuI CTGGAG 1 cut(s) 168
HaeII RGCGCY 2 cut(s) 412, 1323
HaeIII GGCC 2 cut(s) 39, 63
HgaI GACGC 1 cut(s) 253
HhaI GCGC 2 cut(s) 411, 1322
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 7 cut(s) 241, 394, 473, 481, 560, 751, 1264
Hin6I GCGC 2 cut(s) 409, 1320
HinP1I GCGC 2 cut(s) 409, 1320
HincII GTYRAC 1 cut(s) 1189
HindII GTYRAC 1 cut(s) 1189
HinfI GANTC 4 cut(s) 125, 162, 638, 941
HphI GGTGA 1 cut(s) 886
Hpy166II GTNNAC 2 cut(s) 129, 1189
Hpy188I TCNGA 7 cut(s) 32, 297, 814, 972, 994, 1183, 1308
Hpy188III TCNNGA 5 cut(s) 185, 238, 470, 605, 1289
Hpy8I GTNNAC 2 cut(s) 129, 1189
Hpy99I CGWCG 1 cut(s) 20
HpyAV CCTTC 5 cut(s) 74, 467, 809, 864, 874
HpyCH4III ACNGT 4 cut(s) 541, 1196, 1243, 1300
HpyCH4IV ACGT 3 cut(s) 18, 366, 985
HpyF10VI GCNNNNNNNGC 6 cut(s) 57, 417, 757, 973, 1319, 1322
HpyF3I CTNAG 8 cut(s) 56, 485, 643, 722, 752, 969, 1089, 1307
HpySE526I ACGT 3 cut(s) 18, 366, 985
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 7 cut(s) 241, 394, 473, 481, 560, 751, 1264
HspAI GCGC 2 cut(s) 409, 1320
Kzo9I GATC 8 cut(s) 225, 342, 505, 662, 850, 1114, 1137, 1303
LmnI GCTCC 2 cut(s) 1019, 1039
Lsp1109I GCAGC 2 cut(s) 998, 1300
LweI GCATC 1 cut(s) 1207
MaeI CTAG 3 cut(s) 527, 563, 692
MaeII ACGT 3 cut(s) 18, 366, 985
MaeIII GTNAC 4 cut(s) 283, 493, 805, 874
MalI GATC 8 cut(s) 227, 344, 507, 664, 852, 1116, 1139, 1305
MboI GATC 8 cut(s) 225, 342, 505, 662, 850, 1114, 1137, 1303
MboII GAAGA 4 cut(s) 20, 169, 352, 628
MflI RGATCY 6 cut(s) 342, 505, 662, 1114, 1137, 1303
MhlI GDGCHC 3 cut(s) 30, 200, 418
MluCI AATT 2 cut(s) 298, 583
MlyI GAGTC 2 cut(s) 134, 647
Mph1103I ATGCAT 1 cut(s) 53
MroXI GAANNNNTTC 2 cut(s) 51, 161
MseI TTAA 2 cut(s) 138, 1064
MslI CAYNNNNRTG 3 cut(s) 279, 576, 746
MspA1I CMGCKG 2 cut(s) 60, 1325
MspR9I CCNGG 3 cut(s) 707, 1107, 1121
Mva1269I GAATGC 2 cut(s) 51, 53
MvaI CCWGG 3 cut(s) 707, 1107, 1121
MvnI CGCG 1 cut(s) 264
MwoI GCNNNNNNNGC 6 cut(s) 57, 417, 757, 973, 1319, 1322
NdeI CATATG 1 cut(s) 96
NdeII GATC 8 cut(s) 225, 342, 505, 662, 850, 1114, 1137, 1303
NlaIII CATG 7 cut(s) 241, 394, 473, 481, 560, 751, 1264
NlaIV GGNNCC 1 cut(s) 1125
NmuCI GTSAC 2 cut(s) 805, 874
NsiI ATGCAT 1 cut(s) 53
NspI RCATGY 3 cut(s) 560, 751, 1264
NspV TTCGAA 1 cut(s) 515
PaeI GCATGC 1 cut(s) 560
PagI TCATGA 2 cut(s) 237, 469
PciI ACATGT 1 cut(s) 1260
PctI GAATGC 2 cut(s) 51, 53
PdmI GAANNNNTTC 2 cut(s) 51, 161
PfeI GAWTC 2 cut(s) 162, 941
PflMI CCANNNNNTGG 1 cut(s) 686
PkrI GCNGC 4 cut(s) 62, 1013, 1315, 1324
PleI GAGTC 2 cut(s) 133, 646
PpsI GAGTC 2 cut(s) 133, 646
PpuMI RGGWCCY 1 cut(s) 1124
PscI ACATGT 1 cut(s) 1260
Psp5II RGGWCCY 1 cut(s) 1124
Psp6I CCWGG 3 cut(s) 705, 1105, 1119
PspGI CCWGG 3 cut(s) 705, 1105, 1119
PspN4I GGNNCC 1 cut(s) 1125
PspPI GGNCC 2 cut(s) 481, 1124
PspPPI RGGWCCY 1 cut(s) 1124
PsuI RGATCY 6 cut(s) 342, 505, 662, 1114, 1137, 1303
RsaI GTAC 4 cut(s) 90, 354, 521, 1245
RsaNI GTAC 4 cut(s) 89, 353, 520, 1244
RseI CAYNNNNRTG 3 cut(s) 279, 576, 746
SaqAI TTAA 2 cut(s) 138, 1064
SatI GCNGC 4 cut(s) 61, 1012, 1314, 1323
Sau3AI GATC 8 cut(s) 225, 342, 505, 662, 850, 1114, 1137, 1303
Sau96I GGNCC 2 cut(s) 481, 1124
SchI GAGTC 2 cut(s) 134, 647
ScrFI CCNGG 3 cut(s) 707, 1107, 1121
SduI GDGCHC 3 cut(s) 30, 200, 418
SfaNI GCATC 1 cut(s) 1207
SfcI CTRYAG 2 cut(s) 309, 1128
SfuI TTCGAA 1 cut(s) 515
SinI GGWCC 2 cut(s) 481, 1124
SmiMI CAYNNNNRTG 3 cut(s) 279, 576, 746
SmlI CTYRAG 2 cut(s) 83, 199
SmoI CTYRAG 2 cut(s) 83, 199
SphI GCATGC 1 cut(s) 560
Sse9I AATT 2 cut(s) 298, 583
SsiI CCGC 2 cut(s) 60, 1323
SspMI CTAG 3 cut(s) 527, 563, 692
StyD4I CCNGG 3 cut(s) 705, 1105, 1119
TaaI ACNGT 4 cut(s) 541, 1196, 1243, 1300
TaiI ACGT 3 cut(s) 21, 369, 988
TaqI TCGA 1 cut(s) 515
TaqII GACCGA 1 cut(s) 1094
TasI AATT 2 cut(s) 298, 583
TatI WGTACW 1 cut(s) 1243
TauI GCSGC 2 cut(s) 63, 1325
TfiI GAWTC 2 cut(s) 162, 941
Tru1I TTAA 2 cut(s) 138, 1064
Tru9I TTAA 2 cut(s) 138, 1064
TscAI CASTG 2 cut(s) 957, 1199
TseFI GTSAC 2 cut(s) 805, 874
TseI GCWGC 2 cut(s) 1011, 1313
Tsp45I GTSAC 2 cut(s) 805, 874
TspDTI ATGAA 7 cut(s) 21, 226, 486, 538, 638, 798, 1189
TspGWI ACGGA 1 cut(s) 61
TspRI CASTG 2 cut(s) 957, 1199
Van91I CCANNNNNTGG 1 cut(s) 686
VpaK11BI GGWCC 2 cut(s) 481, 1124
XapI RAATTY 1 cut(s) 298
XceI RCATGY 3 cut(s) 560, 751, 1264
XcmI CCANNNNNNNNNTGG 1 cut(s) 238
XmiI GTMKAC 1 cut(s) 128
XmnI GAANNNNTTC 2 cut(s) 51, 161
XspI CTAG 3 cut(s) 527, 563, 692
ZraI GACGTC 1 cut(s) 19
Zsp2I ATGCAT 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.