Rorug02G0525300

Lysyl-tRNA synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000322
Physical Location & Seq
Forward (+)
65556515 .. 65563390
6876 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0525300.1

Sequence Viewer

Length: 849 bp
ATGCTGGCATCGGTTGCTGTAAATCTGAGATCAGCAGCTTCCAGAGCCTTGTCAAACTCTACTTTCTCACTCGTACGGACTCTCCCTCCTCCTTCGCTCTCTCGGTTCTCGGTTCGATCCATGGCGGATTCTGCTGCCCCGTTCAAGAAAGTCCAAATCCAGAGAGACGATACTGCATTTGATGCATATGTTGTTGGCAAAGAAGGTGCTCCTGGGATTGTCGTAGTTCAAGAGTGGTGGGGTGTAGATTTTGAAATAAAGAACCATGCTGTCAAAATTTCTGAACTTGCTCCTGGATTCAAAGCACTTATCCCTGACTTGTATCGGGGAAAGGTTGGTTTGGATGTTGCAGAAGCACAACATTTGATGGAAGGTCTTGACTGGCAAGGTGCGGTAAAGGATATCCAGGCTTCAGTTAATTGGCTGAAAGCAAATGGTTCAAAGAAGGTTGGAGTTACTGGATTCTGCATGGGAGGTGCTCTCTCAATTGCAAGTTCTGTTTTGGTCCCTGAGGTCGATGCTGTTGTTGCATTTTATGGAATACCATCATCAGAGCTTGCAGACCCTGCAAAAGCTAAGGCTCCTATTCAGGCTCATTTTGGAGAGCTTGATAGTTTTGTTGGCTTTTCAGATGTCACGGCTGCCAAGTCTTTGGAGGAGAAGCTGAAAGCATCAGGAGTCCCATATGAGGTGCTCCTGTATCCAGGCAATGCCCATGCTTTCATGAATAGGTCTGAAGACGGTATCAAGCGCAGGAAAGGCATGGGAATGCCTGATGAGGATGAAGCGGCAGTTCAGCTCGCTTGGTCTCGCTTCCAGTCATGGATGACCCGCCATTTGTCTGCTTAA

Protein Analysis

282

Amino Acids

30.33

Weight (kDa)

6.53

Isoelectric Point (pI)

31.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 60 - 279 3.4e-57 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000322)

Species Orthologous Gene IDs
rosa_rugosa Rorug01G0045800 Rorug01G0060200 Rorug01G0078200 Rorug01G0080300 Rorug01G0080500 Rorug01G0093600 Rorug01G0096100 Rorug01G0138500.1 Rorug01G0147900.1 Rorug01G0156300.1 Rorug01G0181600 Rorug01G0183600 Rorug01G0183600 Rorug01G0196000 Rorug01G0206200 Rorug01G0206200 Rorug01G0206300 Rorug01G0206400 Rorug01G0245700 Rorug01G0267300 Rorug01G0268000 Rorug01G0324700 Rorug01G0324800 Rorug02G0034400 Rorug02G0034500 Rorug02G0104900 Rorug02G0224800 Rorug02G0224900 Rorug02G0247400 Rorug02G0261500 Rorug02G0288300 Rorug02G0297300 Rorug02G0297400 Rorug02G0314900 Rorug02G0362700 Rorug02G0438500 Rorug02G0438600 Rorug02G0438700 Rorug02G0438800 Rorug02G0525300 Rorug02G0540100 Rorug03G0147100 Rorug03G0150900 Rorug03G0171600 Rorug03G0182000 Rorug03G0200200 Rorug03G0201100 Rorug03G0218400 Rorug03G0218700 Rorug03G0268200 Rorug03G0285500 Rorug03G0296600 Rorug03G0296700 Rorug03G0296800 Rorug03G0341800 Rorug03G0347200 Rorug03G0356300 Rorug04G0029100 Rorug04G0036300 Rorug04G0046500 Rorug04G0070100 Rorug04G0098900 Rorug04G0104400 Rorug04G0167800 Rorug04G0209300 Rorug04G0210700 Rorug04G0210800 Rorug04G0212500 Rorug05G0109400 Rorug05G0148500 Rorug05G0148800 Rorug05G0160200 Rorug05G0184600 Rorug05G0206100 Rorug05G0208500 Rorug05G0208500 Rorug05G0209500 Rorug05G0235800 Rorug05G0239200 Rorug05G0301100 Rorug05G0427000 Rorug05G0451700 Rorug05G0462300 Rorug05G0467300 Rorug05G0524100 Rorug05G0527400 Rorug05G0527500 Rorug05G0561800 Rorug05G0567700 Rorug05G0574200 Rorug06G0000900 Rorug06G0018700.1 Rorug06G0025100 Rorug06G0025200 Rorug06G0025500 Rorug06G0041300 Rorug06G0048100 Rorug06G0050800 Rorug06G0053900 Rorug06G0075200 Rorug06G0080600 Rorug06G0087500.1 Rorug06G0090000 Rorug06G0141200 Rorug06G0218300 Rorug07G0106700 Rorug07G0125500 Rorug07G0185500 Rorug07G0194000 Rorug07G0196600 Rorug07G0200600 Rorug07G0217300 Rorug07G0250300 Rorug07G0301900 Rorug07G0309100.1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 125, 392, 788, 832
AclWI GGATC 1 cut(s) 111
AcsI RAATTY 1 cut(s) 276
AcuI CTGAAG 2 cut(s) 396, 756
AfaI GTAC 1 cut(s) 75
AfiI CCNNNNNNNGG 1 cut(s) 688
AgsI TTSAA 5 cut(s) 145, 230, 254, 301, 441
AjnI CCWGG 4 cut(s) 211, 292, 405, 703
AluBI AGCT 6 cut(s) 38, 556, 575, 607, 664, 799
AluI AGCT 6 cut(s) 38, 556, 575, 607, 664, 799
Alw21I GWGCWC 3 cut(s) 211, 481, 696
Alw26I GTCTC 2 cut(s) 159, 813
AlwI GGATC 1 cut(s) 111
AlwNI CAGNNNCTG 1 cut(s) 566
ApeKI GCWGC 3 cut(s) 35, 134, 641
ApoI RAATTY 1 cut(s) 276
ArsI GACNNNNNNTTYG 1 cut(s) 820
AspLEI GCGC 1 cut(s) 753
AspS9I GGNCC 1 cut(s) 505
AvaII GGWCC 1 cut(s) 505
AxyI CCTNAGG 1 cut(s) 510
BbsI GAAGAC 1 cut(s) 744
Bbv12I GWGCWC 3 cut(s) 211, 481, 696
BbvI GCAGC 3 cut(s) 47, 121, 628
BccI CCATC 2 cut(s) 361, 553
BceAI ACGGC 1 cut(s) 654
BciT130I CCWGG 4 cut(s) 213, 294, 407, 705
BciVI GTATCC 1 cut(s) 711
BcoDI GTCTC 2 cut(s) 159, 813
BfuI GTATCC 1 cut(s) 711
BisI GCNGC 4 cut(s) 36, 135, 642, 789
BlsI GCNGC 4 cut(s) 37, 136, 643, 790
Bme1390I CCNGG 4 cut(s) 213, 294, 407, 705
Bme18I GGWCC 1 cut(s) 505
BmgT120I GGNCC 1 cut(s) 505
BmiI GGNNCC 2 cut(s) 507, 582
BmrFI CCNGG 4 cut(s) 213, 294, 407, 705
BmsI GCATC 4 cut(s) 17, 172, 508, 680
BpiI GAAGAC 1 cut(s) 744
BplI GAGNNNNNCTC 2 cut(s) 465, 497
Bpu10I CCTNAGC 1 cut(s) 576
BsaI GGTCTC 1 cut(s) 813
BsaJI CCNNGG 2 cut(s) 120, 212
BsaXI ACNNNNNCTCC 4 cut(s) 66, 70, 96, 100
Bsc4I CCNNNNNNNGG 1 cut(s) 688
Bse1I ACTGG 3 cut(s) 386, 463, 817
Bse21I CCTNAGG 1 cut(s) 510
Bse3DI GCAATG 1 cut(s) 715
BseBI CCWGG 4 cut(s) 213, 294, 407, 705
BseDI CCNNGG 2 cut(s) 120, 212
BseGI GGATG 3 cut(s) 349, 787, 831
BseLI CCNNNNNNNGG 1 cut(s) 688
BseMI GCAATG 1 cut(s) 715
BseMII CTCAG 2 cut(s) 17, 501
BseNI ACTGG 3 cut(s) 386, 463, 817
BseRI GAGGAG 2 cut(s) 78, 671
BseXI GCAGC 3 cut(s) 47, 121, 628
BsiHKAI GWGCWC 3 cut(s) 211, 481, 696
BsiWI CGTACG 1 cut(s) 73
BslFI GGGAC 2 cut(s) 491, 665
BslI CCNNNNNNNGG 1 cut(s) 688
BsmAI GTCTC 2 cut(s) 159, 813
BsmBI CGTCTC 1 cut(s) 159
BsmFI GGGAC 2 cut(s) 491, 665
BsmI GAATGC 1 cut(s) 774
Bso31I GGTCTC 1 cut(s) 813
Bsp1286I GDGCHC 3 cut(s) 211, 481, 696
Bsp143I GATC 2 cut(s) 29, 116
Bsp19I CCATGG 1 cut(s) 120
BspACI CCGC 4 cut(s) 125, 392, 788, 832
BspCNI CTCAG 2 cut(s) 18, 502
BspHI TCATGA 1 cut(s) 723
BspLI GGNNCC 2 cut(s) 507, 582
BspPI GGATC 1 cut(s) 111
BspTNI GGTCTC 1 cut(s) 813
BsrDI GCAATG 1 cut(s) 715
BsrI ACTGG 3 cut(s) 386, 463, 817
BssECI CCNNGG 2 cut(s) 120, 212
BssMI GATC 2 cut(s) 29, 116
BssT1I CCWWGG 1 cut(s) 120
Bst2UI CCWGG 4 cut(s) 213, 294, 407, 705
Bst4CI ACNGT 1 cut(s) 743
BstAPI GCANNNNNTGC 3 cut(s) 14, 182, 566
BstC8I GCNNGC 3 cut(s) 6, 558, 801
BstDEI CTNAG 3 cut(s) 26, 510, 576
BstDSI CCRYGG 1 cut(s) 120
BstF5I GGATG 3 cut(s) 349, 787, 831
BstHHI GCGC 1 cut(s) 753
BstKTI GATC 2 cut(s) 32, 119
BstMAI GTCTC 2 cut(s) 159, 813
BstMBI GATC 2 cut(s) 29, 116
BstMWI GCNNNNNNNGC 7 cut(s) 14, 44, 131, 182, 527, 566, 759
BstNI CCWGG 4 cut(s) 213, 294, 407, 705
BstSCI CCNGG 4 cut(s) 211, 292, 405, 703
BstV1I GCAGC 3 cut(s) 47, 121, 628
BstV2I GAAGAC 1 cut(s) 744
BstXI CCANNNNNNTGG 1 cut(s) 652
Bsu36I CCTNAGG 1 cut(s) 510
BsuI GTATCC 1 cut(s) 711
BtgI CCRYGG 1 cut(s) 120
BtsCI GGATG 3 cut(s) 349, 787, 831
Cac8I GCNNGC 3 cut(s) 6, 558, 801
CaiI CAGNNNCTG 1 cut(s) 566
CciI TCATGA 1 cut(s) 723
CfoI GCGC 1 cut(s) 753
Cfr13I GGNCC 1 cut(s) 505
Csp6I GTAC 1 cut(s) 74
CspCI CAANNNNNGTGG 2 cut(s) 218, 253
CviAII CATG 7 cut(s) 121, 266, 469, 716, 724, 763, 822
CviQI GTAC 1 cut(s) 74
DdeI CTNAG 3 cut(s) 26, 510, 576
DpnI GATC 2 cut(s) 31, 118
DpnII GATC 2 cut(s) 29, 116
EciI GGCGGA 1 cut(s) 140
Eco130I CCWWGG 1 cut(s) 120
Eco31I GGTCTC 1 cut(s) 813
Eco32I GATATC 1 cut(s) 403
Eco47I GGWCC 1 cut(s) 505
Eco57I CTGAAG 2 cut(s) 396, 756
Eco81I CCTNAGG 1 cut(s) 510
EcoRII CCWGG 4 cut(s) 211, 292, 405, 703
EcoRV GATATC 1 cut(s) 403
EcoT14I CCWWGG 1 cut(s) 120
EcoT22I ATGCAT 1 cut(s) 187
ErhI CCWWGG 1 cut(s) 120
Esp3I CGTCTC 1 cut(s) 159
FaeI CATG 7 cut(s) 124, 269, 472, 719, 727, 766, 825
FaqI GGGAC 2 cut(s) 491, 665
FatI CATG 7 cut(s) 120, 265, 468, 715, 723, 762, 821
FauI CCCGC 1 cut(s) 839
FauNDI CATATG 2 cut(s) 187, 685
Fnu4HI GCNGC 4 cut(s) 36, 135, 642, 789
FokI GGATG 3 cut(s) 356, 794, 838
Fsp4HI GCNGC 4 cut(s) 36, 135, 642, 789
GlaI GCGC 1 cut(s) 752
GluI GCNGC 4 cut(s) 36, 135, 642, 789
HhaI GCGC 1 cut(s) 753
Hin1II CATG 7 cut(s) 124, 269, 472, 719, 727, 766, 825
Hin6I GCGC 1 cut(s) 751
HinP1I GCGC 1 cut(s) 751
HinfI GANTC 5 cut(s) 79, 128, 297, 462, 678
Hpy188I TCNGA 5 cut(s) 27, 283, 553, 631, 736
Hpy188III TCNNGA 7 cut(s) 42, 145, 160, 230, 377, 675, 724
HpyAV CCTTC 4 cut(s) 102, 197, 365, 439
HpyCH4III ACNGT 1 cut(s) 743
HpyCH4V TGCA 8 cut(s) 176, 185, 350, 468, 491, 530, 560, 569
HpyF10VI GCNNNNNNNGC 7 cut(s) 14, 44, 131, 182, 527, 566, 759
HpyF3I CTNAG 3 cut(s) 26, 510, 576
Hsp92II CATG 7 cut(s) 124, 269, 472, 719, 727, 766, 825
HspAI GCGC 1 cut(s) 751
Kzo9I GATC 2 cut(s) 29, 116
LmnI GCTCC 4 cut(s) 214, 295, 586, 699
Lsp1109I GCAGC 3 cut(s) 47, 121, 628
LweI GCATC 4 cut(s) 17, 172, 508, 680
MaeIII GTNAC 2 cut(s) 454, 634
MalI GATC 2 cut(s) 31, 118
MboI GATC 2 cut(s) 29, 116
MboII GAAGA 1 cut(s) 749
MfeI CAATTG 1 cut(s) 486
MhlI GDGCHC 3 cut(s) 211, 481, 696
MluCI AATT 3 cut(s) 276, 418, 486
MlyI GAGTC 2 cut(s) 73, 687
MmeI TCCRAC 1 cut(s) 430
MnlI CCTC 7 cut(s) 96, 99, 467, 505, 649, 682, 772
Mph1103I ATGCAT 1 cut(s) 187
MseI TTAA 2 cut(s) 417, 847
MslI CAYNNNNRTG 1 cut(s) 767
MspR9I CCNGG 4 cut(s) 213, 294, 407, 705
MunI CAATTG 1 cut(s) 486
Mva1269I GAATGC 1 cut(s) 774
MvaI CCWGG 4 cut(s) 213, 294, 407, 705
MwoI GCNNNNNNNGC 7 cut(s) 14, 44, 131, 182, 527, 566, 759
NcoI CCATGG 1 cut(s) 120
NdeI CATATG 2 cut(s) 187, 685
NdeII GATC 2 cut(s) 29, 116
NlaIII CATG 7 cut(s) 124, 269, 472, 719, 727, 766, 825
NlaIV GGNNCC 2 cut(s) 507, 582
NmuCI GTSAC 1 cut(s) 634
NsiI ATGCAT 1 cut(s) 187
PagI TCATGA 1 cut(s) 723
PctI GAATGC 1 cut(s) 774
PfeI GAWTC 3 cut(s) 128, 297, 462
Pfl23II CGTACG 1 cut(s) 73
PfoI TCCNGGA 1 cut(s) 292
PkrI GCNGC 4 cut(s) 37, 136, 643, 790
PleI GAGTC 2 cut(s) 73, 686
PpsI GAGTC 2 cut(s) 73, 686
Psp6I CCWGG 4 cut(s) 211, 292, 405, 703
PspGI CCWGG 4 cut(s) 211, 292, 405, 703
PspLI CGTACG 1 cut(s) 73
PspN4I GGNNCC 2 cut(s) 507, 582
PspPI GGNCC 1 cut(s) 505
PstNI CAGNNNCTG 1 cut(s) 566
RsaI GTAC 1 cut(s) 75
RsaNI GTAC 1 cut(s) 74
RseI CAYNNNNRTG 1 cut(s) 767
SaqAI TTAA 2 cut(s) 417, 847
SatI GCNGC 4 cut(s) 36, 135, 642, 789
Sau3AI GATC 2 cut(s) 29, 116
Sau96I GGNCC 1 cut(s) 505
SchI GAGTC 2 cut(s) 73, 687
ScrFI CCNGG 4 cut(s) 213, 294, 407, 705
SduI GDGCHC 3 cut(s) 211, 481, 696
SfaNI GCATC 4 cut(s) 17, 172, 508, 680
SinI GGWCC 1 cut(s) 505
SmiMI CAYNNNNRTG 1 cut(s) 767
Sse9I AATT 3 cut(s) 276, 418, 486
SsiI CCGC 4 cut(s) 125, 392, 788, 832
StyD4I CCNGG 4 cut(s) 211, 292, 405, 703
StyI CCWWGG 1 cut(s) 120
TaaI ACNGT 1 cut(s) 743
TaqI TCGA 2 cut(s) 115, 516
TasI AATT 3 cut(s) 276, 418, 486
TauI GCSGC 1 cut(s) 791
TfiI GAWTC 3 cut(s) 128, 297, 462
Tru1I TTAA 2 cut(s) 417, 847
Tru9I TTAA 2 cut(s) 417, 847
TseFI GTSAC 1 cut(s) 634
TseI GCWGC 3 cut(s) 35, 134, 641
Tsp45I GTSAC 1 cut(s) 634
TspDTI ATGAA 3 cut(s) 712, 740, 798
TspGWI ACGGA 1 cut(s) 91
VpaK11BI GGWCC 1 cut(s) 505
XapI RAATTY 1 cut(s) 276
Zsp2I ATGCAT 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.