Purple acid phosphatase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 551, 718 |
| AccII | CGCG | 3 cut(s) | 425, 613, 716 |
| AciI | CCGC | 4 cut(s) | 168, 252, 377, 716 |
| AclWI | GGATC | 2 cut(s) | 278, 592 |
| AcsI | RAATTY | 1 cut(s) | 334 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 159, 308 |
| AgsI | TTSAA | 2 cut(s) | 235, 605 |
| AjnI | CCWGG | 1 cut(s) | 721 |
| AleI | CACNNNNGTG | 1 cut(s) | 292 |
| AluBI | AGCT | 4 cut(s) | 215, 437, 502, 663 |
| AluI | AGCT | 4 cut(s) | 215, 437, 502, 663 |
| AlwI | GGATC | 2 cut(s) | 278, 592 |
| AoxI | GGCC | 1 cut(s) | 622 |
| ApeKI | GCWGC | 1 cut(s) | 212 |
| ApoI | RAATTY | 1 cut(s) | 334 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 20 |
| AspLEI | GCGC | 3 cut(s) | 427, 615, 644 |
| AspS9I | GGNCC | 2 cut(s) | 58, 622 |
| AsuHPI | GGTGA | 3 cut(s) | 43, 188, 569 |
| AvaII | GGWCC | 1 cut(s) | 58 |
| BanI | GGYRCC | 2 cut(s) | 551, 718 |
| BanII | GRGCYC | 1 cut(s) | 358 |
| BbsI | GAAGAC | 1 cut(s) | 696 |
| BbvI | GCAGC | 1 cut(s) | 224 |
| BccI | CCATC | 1 cut(s) | 360 |
| BceAI | ACGGC | 1 cut(s) | 145 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 61, 95, 98, 132 |
| BciT130I | CCWGG | 1 cut(s) | 723 |
| BfaI | CTAG | 1 cut(s) | 203 |
| BisI | GCNGC | 2 cut(s) | 213, 717 |
| BlsI | GCNGC | 2 cut(s) | 214, 718 |
| Bme1390I | CCNGG | 1 cut(s) | 723 |
| Bme18I | GGWCC | 1 cut(s) | 58 |
| BmgT120I | GGNCC | 2 cut(s) | 58, 622 |
| BmiI | GGNNCC | 3 cut(s) | 553, 623, 720 |
| BmrFI | CCNGG | 1 cut(s) | 723 |
| BmrI | ACTGGG | 1 cut(s) | 361 |
| BmsI | GCATC | 2 cut(s) | 100, 306 |
| BmuI | ACTGGG | 1 cut(s) | 361 |
| BpiI | GAAGAC | 1 cut(s) | 696 |
| BpuEI | CTTGAG | 1 cut(s) | 342 |
| BsaJI | CCNNGG | 1 cut(s) | 722 |
| BsaWI | WCCGGW | 2 cut(s) | 448, 593 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 159, 308 |
| Bse118I | RCCGGY | 1 cut(s) | 554 |
| Bse1I | ACTGG | 4 cut(s) | 107, 356, 524, 684 |
| BseBI | CCWGG | 1 cut(s) | 723 |
| BseDI | CCNNGG | 1 cut(s) | 722 |
| BseGI | GGATG | 2 cut(s) | 297, 371 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 159, 308 |
| BseNI | ACTGG | 4 cut(s) | 107, 356, 524, 684 |
| BseXI | GCAGC | 1 cut(s) | 224 |
| Bsh1236I | CGCG | 3 cut(s) | 425, 613, 716 |
| BshFI | GGCC | 1 cut(s) | 624 |
| BshNI | GGYRCC | 2 cut(s) | 551, 718 |
| BsiSI | CCGG | 3 cut(s) | 449, 555, 594 |
| BslFI | GGGAC | 1 cut(s) | 452 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 159, 308 |
| BsmFI | GGGAC | 1 cut(s) | 452 |
| BsnI | GGCC | 1 cut(s) | 624 |
| Bsp1286I | GDGCHC | 1 cut(s) | 358 |
| Bsp143I | GATC | 6 cut(s) | 178, 267, 283, 315, 385, 584 |
| BspACI | CCGC | 4 cut(s) | 168, 252, 377, 716 |
| BspANI | GGCC | 1 cut(s) | 624 |
| BspFNI | CGCG | 3 cut(s) | 425, 613, 716 |
| BspLI | GGNNCC | 3 cut(s) | 553, 623, 720 |
| BspPI | GGATC | 2 cut(s) | 278, 592 |
| BspT107I | GGYRCC | 2 cut(s) | 551, 718 |
| BsrFI | RCCGGY | 1 cut(s) | 554 |
| BsrI | ACTGG | 4 cut(s) | 107, 356, 524, 684 |
| BssAI | RCCGGY | 1 cut(s) | 554 |
| BssECI | CCNNGG | 1 cut(s) | 722 |
| BssMI | GATC | 6 cut(s) | 178, 267, 283, 315, 385, 584 |
| Bst2UI | CCWGG | 1 cut(s) | 723 |
| Bst4CI | ACNGT | 1 cut(s) | 37 |
| Bst6I | CTCTTC | 1 cut(s) | 188 |
| BstC8I | GCNNGC | 2 cut(s) | 166, 310 |
| BstDEI | CTNAG | 2 cut(s) | 218, 649 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 306 |
| BstF5I | GGATG | 2 cut(s) | 297, 371 |
| BstFNI | CGCG | 3 cut(s) | 425, 613, 716 |
| BstHHI | GCGC | 3 cut(s) | 427, 615, 644 |
| BstKTI | GATC | 6 cut(s) | 181, 270, 286, 318, 388, 587 |
| BstMBI | GATC | 6 cut(s) | 178, 267, 283, 315, 385, 584 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 621 |
| BstNI | CCWGG | 1 cut(s) | 723 |
| BstNSI | RCATGY | 1 cut(s) | 119 |
| BstSCI | CCNGG | 1 cut(s) | 721 |
| BstUI | CGCG | 3 cut(s) | 425, 613, 716 |
| BstV1I | GCAGC | 1 cut(s) | 224 |
| BstV2I | GAAGAC | 1 cut(s) | 696 |
| BstX2I | RGATCY | 1 cut(s) | 584 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 351, 729 |
| BstYI | RGATCY | 1 cut(s) | 584 |
| BsuRI | GGCC | 1 cut(s) | 624 |
| BtsCI | GGATG | 2 cut(s) | 297, 371 |
| BtsIMutI | CAGTG | 2 cut(s) | 42, 691 |
| Cac8I | GCNNGC | 2 cut(s) | 166, 310 |
| CfoI | GCGC | 3 cut(s) | 427, 615, 644 |
| Cfr10I | RCCGGY | 1 cut(s) | 554 |
| Cfr13I | GGNCC | 2 cut(s) | 58, 622 |
| CseI | GACGC | 1 cut(s) | 265 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 529, 564 |
| CviAII | CATG | 8 cut(s) | 67, 98, 116, 136, 154, 271, 281, 403 |
| CviJI | RGCY | 8 cut(s) | 164, 215, 356, 437, 502, 601, 624, 663 |
| CviKI_1 | RGCY | 8 cut(s) | 164, 215, 356, 437, 502, 601, 624, 663 |
| DdeI | CTNAG | 2 cut(s) | 218, 649 |
| DpnI | GATC | 6 cut(s) | 180, 269, 285, 317, 387, 586 |
| DpnII | GATC | 6 cut(s) | 178, 267, 283, 315, 385, 584 |
| Eam1104I | CTCTTC | 1 cut(s) | 188 |
| EarI | CTCTTC | 1 cut(s) | 188 |
| Eco24I | GRGCYC | 1 cut(s) | 358 |
| Eco47I | GGWCC | 1 cut(s) | 58 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 306 |
| EcoRII | CCWGG | 1 cut(s) | 721 |
| EcoT38I | GRGCYC | 1 cut(s) | 358 |
| FaeI | CATG | 8 cut(s) | 70, 101, 119, 139, 157, 274, 284, 406 |
| FaqI | GGGAC | 1 cut(s) | 452 |
| FatI | CATG | 8 cut(s) | 66, 97, 115, 135, 153, 270, 280, 402 |
| FauI | CCCGC | 1 cut(s) | 384 |
| FauNDI | CATATG | 1 cut(s) | 748 |
| Fnu4HI | GCNGC | 2 cut(s) | 213, 717 |
| FokI | GGATG | 2 cut(s) | 284, 378 |
| FriOI | GRGCYC | 1 cut(s) | 358 |
| Fsp4HI | GCNGC | 2 cut(s) | 213, 717 |
| FspBI | CTAG | 1 cut(s) | 203 |
| GlaI | GCGC | 3 cut(s) | 426, 614, 643 |
| GluI | GCNGC | 2 cut(s) | 213, 717 |
| HaeIII | GGCC | 1 cut(s) | 624 |
| HapII | CCGG | 3 cut(s) | 449, 555, 594 |
| HgaI | GACGC | 1 cut(s) | 265 |
| HhaI | GCGC | 3 cut(s) | 427, 615, 644 |
| Hin1II | CATG | 8 cut(s) | 70, 101, 119, 139, 157, 274, 284, 406 |
| Hin6I | GCGC | 3 cut(s) | 425, 613, 642 |
| HinP1I | GCGC | 3 cut(s) | 425, 613, 642 |
| HincII | GTYRAC | 1 cut(s) | 565 |
| HindII | GTYRAC | 1 cut(s) | 565 |
| HindIII | AAGCTT | 1 cut(s) | 661 |
| HinfI | GANTC | 3 cut(s) | 88, 465, 576 |
| HpaI | GTTAAC | 1 cut(s) | 565 |
| HpaII | CCGG | 3 cut(s) | 449, 555, 594 |
| HphI | GGTGA | 3 cut(s) | 43, 188, 569 |
| Hpy166II | GTNNAC | 4 cut(s) | 344, 414, 538, 565 |
| Hpy188I | TCNGA | 2 cut(s) | 26, 575 |
| Hpy188III | TCNNGA | 4 cut(s) | 13, 92, 203, 265 |
| Hpy8I | GTNNAC | 4 cut(s) | 344, 414, 538, 565 |
| HpyAV | CCTTC | 1 cut(s) | 133 |
| HpyCH4III | ACNGT | 1 cut(s) | 37 |
| HpyCH4V | TGCA | 7 cut(s) | 113, 119, 157, 297, 308, 329, 372 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 621 |
| HpyF3I | CTNAG | 2 cut(s) | 218, 649 |
| Hsp92II | CATG | 8 cut(s) | 70, 101, 119, 139, 157, 274, 284, 406 |
| HspAI | GCGC | 3 cut(s) | 425, 613, 642 |
| KspAI | GTTAAC | 1 cut(s) | 565 |
| Kzo9I | GATC | 6 cut(s) | 178, 267, 283, 315, 385, 584 |
| Lsp1109I | GCAGC | 1 cut(s) | 224 |
| LweI | GCATC | 2 cut(s) | 100, 306 |
| MaeI | CTAG | 1 cut(s) | 203 |
| MaeIII | GTNAC | 3 cut(s) | 31, 676, 761 |
| MalI | GATC | 6 cut(s) | 180, 269, 285, 317, 387, 586 |
| MboI | GATC | 6 cut(s) | 178, 267, 283, 315, 385, 584 |
| MboII | GAAGA | 2 cut(s) | 175, 701 |
| MfeI | CAATTG | 1 cut(s) | 616 |
| MflI | RGATCY | 1 cut(s) | 584 |
| MhlI | GDGCHC | 1 cut(s) | 358 |
| MluCI | AATT | 5 cut(s) | 321, 334, 589, 616, 727 |
| MlyI | GAGTC | 2 cut(s) | 97, 474 |
| MmeI | TCCRAC | 1 cut(s) | 211 |
| MnlI | CCTC | 4 cut(s) | 22, 356, 479, 745 |
| MroXI | GAANNNNTTC | 1 cut(s) | 20 |
| MseI | TTAA | 4 cut(s) | 245, 492, 504, 564 |
| MslI | CAYNNNNRTG | 2 cut(s) | 292, 407 |
| MspI | CCGG | 3 cut(s) | 449, 555, 594 |
| MspR9I | CCNGG | 1 cut(s) | 723 |
| MunI | CAATTG | 1 cut(s) | 616 |
| MvaI | CCWGG | 1 cut(s) | 723 |
| MvnI | CGCG | 3 cut(s) | 425, 613, 716 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 621 |
| NdeI | CATATG | 1 cut(s) | 748 |
| NdeII | GATC | 6 cut(s) | 178, 267, 283, 315, 385, 584 |
| NlaIII | CATG | 8 cut(s) | 70, 101, 119, 139, 157, 274, 284, 406 |
| NlaIV | GGNNCC | 3 cut(s) | 553, 623, 720 |
| NmeAIII | GCCGAG | 1 cut(s) | 488 |
| NmuCI | GTSAC | 2 cut(s) | 31, 676 |
| NspI | RCATGY | 1 cut(s) | 119 |
| OliI | CACNNNNGTG | 1 cut(s) | 292 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 390 |
| PdmI | GAANNNNTTC | 1 cut(s) | 20 |
| PfeI | GAWTC | 1 cut(s) | 576 |
| PkrI | GCNGC | 2 cut(s) | 214, 718 |
| PleI | GAGTC | 2 cut(s) | 96, 473 |
| PpsI | GAGTC | 2 cut(s) | 96, 473 |
| Psp6I | CCWGG | 1 cut(s) | 721 |
| PspGI | CCWGG | 1 cut(s) | 721 |
| PspN4I | GGNNCC | 3 cut(s) | 553, 623, 720 |
| PspPI | GGNCC | 2 cut(s) | 58, 622 |
| PsuI | RGATCY | 1 cut(s) | 584 |
| RseI | CAYNNNNRTG | 2 cut(s) | 292, 407 |
| SaqAI | TTAA | 4 cut(s) | 245, 492, 504, 564 |
| SatI | GCNGC | 2 cut(s) | 213, 717 |
| Sau3AI | GATC | 6 cut(s) | 178, 267, 283, 315, 385, 584 |
| Sau96I | GGNCC | 2 cut(s) | 58, 622 |
| SchI | GAGTC | 2 cut(s) | 97, 474 |
| ScrFI | CCNGG | 1 cut(s) | 723 |
| SduI | GDGCHC | 1 cut(s) | 358 |
| SetI | ASST | 7 cut(s) | 33, 125, 196, 217, 439, 504, 665 |
| SfaNI | GCATC | 2 cut(s) | 100, 306 |
| SinI | GGWCC | 1 cut(s) | 58 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 292, 407 |
| SmlI | CTYRAG | 1 cut(s) | 357 |
| SmoI | CTYRAG | 1 cut(s) | 357 |
| Sse9I | AATT | 5 cut(s) | 321, 334, 589, 616, 727 |
| SsiI | CCGC | 4 cut(s) | 168, 252, 377, 716 |
| SspMI | CTAG | 1 cut(s) | 203 |
| StyD4I | CCNGG | 1 cut(s) | 721 |
| TaaI | ACNGT | 1 cut(s) | 37 |
| TaqI | TCGA | 2 cut(s) | 264, 393 |
| TaqII | GACCGA | 1 cut(s) | 46 |
| TasI | AATT | 5 cut(s) | 321, 334, 589, 616, 727 |
| TauI | GCSGC | 1 cut(s) | 719 |
| TfiI | GAWTC | 1 cut(s) | 576 |
| Tru1I | TTAA | 4 cut(s) | 245, 492, 504, 564 |
| Tru9I | TTAA | 4 cut(s) | 245, 492, 504, 564 |
| TscAI | CASTG | 2 cut(s) | 42, 691 |
| TseFI | GTSAC | 2 cut(s) | 31, 676 |
| TseI | GCWGC | 1 cut(s) | 212 |
| Tsp45I | GTSAC | 2 cut(s) | 31, 676 |
| TspDTI | ATGAA | 2 cut(s) | 31, 152 |
| TspRI | CASTG | 2 cut(s) | 42, 691 |
| VpaK11BI | GGWCC | 1 cut(s) | 58 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 306 |
| XapI | RAATTY | 1 cut(s) | 334 |
| XbaI | TCTAGA | 1 cut(s) | 202 |
| XceI | RCATGY | 1 cut(s) | 119 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 194 |
| XmnI | GAANNNNTTC | 1 cut(s) | 20 |
| XspI | CTAG | 1 cut(s) | 203 |