Rorug07G0196600

At5g49945-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000322
Physical Location & Seq
Forward (+)
16936731 .. 16941828
5098 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0196600.1

Sequence Viewer

Length: 549 bp
ATGTCGGCTCTCAAAGACCAGGATCCTCGCGTCCATGCCATCAAAACCAAGATTCGAGTCGTCCCCAATTTCCCCAAACCCGGAATTATGTTCCAAGATATCACAACTTTGTTGCTAGACCCAAAAGCCTTCAAGGACACAATCGATATGTTCGTGGAGAGATACAAAGGCAAAAACATTTCAGTGGTTGCAGGAATTGAGGCTCGAGGTTTCATTTTTGGTCCTCCCATTGCGTTGGCAATTGGAGCAAAGTTTGTTCCCCTGAGAAAACCAAGGAAATTGCCTGGTGATGTTATTTTTGAAGAGTACACTTTGGAATATGGAAGGGACCGTCTTGAGATGCATGTTGGAGCAGTAGAACAGGGTGATCGTGCTCTGGTAGTTGATGATTTAATAGCCACAGGAGGCACGCTCTGTGCTGCAATGAGTTTATTGGAACGTGTTGGAGCAGAAGTAGTTGAATGTGCCTGCCTTATTGAGTTGCCAGATTTACAGGGTCGTGAGCGATTGAATGGCAAACCATTGTATGTACTGGTGGAGTCCCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046872
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1682

Protein Analysis

182

Amino Acids

20.11

Weight (kDa)

6.32

Isoelectric Point (pI)

30.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pribosyltran PF00156 41 - 164 2.1e-18 Phosphoribosyl transferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000322)

Species Orthologous Gene IDs
rosa_rugosa Rorug01G0045800 Rorug01G0060200 Rorug01G0078200 Rorug01G0080300 Rorug01G0080500 Rorug01G0093600 Rorug01G0096100 Rorug01G0138500.1 Rorug01G0147900.1 Rorug01G0156300.1 Rorug01G0181600 Rorug01G0183600 Rorug01G0183600 Rorug01G0196000 Rorug01G0206200 Rorug01G0206200 Rorug01G0206300 Rorug01G0206400 Rorug01G0245700 Rorug01G0267300 Rorug01G0268000 Rorug01G0324700 Rorug01G0324800 Rorug02G0034400 Rorug02G0034500 Rorug02G0104900 Rorug02G0224800 Rorug02G0224900 Rorug02G0247400 Rorug02G0261500 Rorug02G0288300 Rorug02G0297300 Rorug02G0297400 Rorug02G0314900 Rorug02G0362700 Rorug02G0438500 Rorug02G0438600 Rorug02G0438700 Rorug02G0438800 Rorug02G0525300 Rorug02G0540100 Rorug03G0147100 Rorug03G0150900 Rorug03G0171600 Rorug03G0182000 Rorug03G0200200 Rorug03G0201100 Rorug03G0218400 Rorug03G0218700 Rorug03G0268200 Rorug03G0285500 Rorug03G0296600 Rorug03G0296700 Rorug03G0296800 Rorug03G0341800 Rorug03G0347200 Rorug03G0356300 Rorug04G0029100 Rorug04G0036300 Rorug04G0046500 Rorug04G0070100 Rorug04G0098900 Rorug04G0104400 Rorug04G0167800 Rorug04G0209300 Rorug04G0210700 Rorug04G0210800 Rorug04G0212500 Rorug05G0109400 Rorug05G0148500 Rorug05G0148800 Rorug05G0160200 Rorug05G0184600 Rorug05G0206100 Rorug05G0208500 Rorug05G0208500 Rorug05G0209500 Rorug05G0235800 Rorug05G0239200 Rorug05G0301100 Rorug05G0427000 Rorug05G0451700 Rorug05G0462300 Rorug05G0467300 Rorug05G0524100 Rorug05G0527400 Rorug05G0527500 Rorug05G0561800 Rorug05G0567700 Rorug05G0574200 Rorug06G0000900 Rorug06G0018700.1 Rorug06G0025100 Rorug06G0025200 Rorug06G0025500 Rorug06G0041300 Rorug06G0048100 Rorug06G0050800 Rorug06G0053900 Rorug06G0075200 Rorug06G0080600 Rorug06G0087500.1 Rorug06G0090000 Rorug06G0141200 Rorug06G0218300 Rorug07G0106700 Rorug07G0125500 Rorug07G0185500 Rorug07G0194000 Rorug07G0196600 Rorug07G0200600 Rorug07G0217300 Rorug07G0250300 Rorug07G0301900 Rorug07G0309100.1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 30
AclWI GGATC 2 cut(s) 17, 30
AfaI GTAC 2 cut(s) 308, 531
AfiI CCNNNNNNNGG 1 cut(s) 80
AflIII ACRYGT 1 cut(s) 439
AgsI TTSAA 4 cut(s) 133, 302, 461, 511
AjnI CCWGG 2 cut(s) 18, 283
Alw21I GWGCWC 1 cut(s) 376
AlwI GGATC 2 cut(s) 17, 30
Ama87I CYCGRG 1 cut(s) 204
ApeKI GCWGC 1 cut(s) 419
AspS9I GGNCC 2 cut(s) 221, 328
AsuC2I CCSGG 1 cut(s) 81
AsuHPI GGTGA 2 cut(s) 299, 377
AvaI CYCGRG 1 cut(s) 204
AvaII GGWCC 2 cut(s) 221, 328
BamHI GGATCC 1 cut(s) 22
Bbv12I GWGCWC 1 cut(s) 376
BbvI GCAGC 1 cut(s) 406
BccI CCATC 1 cut(s) 47
BciT130I CCWGG 2 cut(s) 20, 285
BcnI CCSGG 1 cut(s) 81
BfaI CTAG 1 cut(s) 116
BisI GCNGC 1 cut(s) 420
BlsI GCNGC 1 cut(s) 421
Bme1390I CCNGG 3 cut(s) 20, 81, 285
Bme18I GGWCC 2 cut(s) 221, 328
BmeT110I CYCGRG 1 cut(s) 204
BmgT120I GGNCC 2 cut(s) 221, 328
BmiI GGNNCC 2 cut(s) 24, 329
BmrFI CCNGG 3 cut(s) 20, 81, 285
BmsI GCATC 1 cut(s) 330
BplI GAGNNNNNCTC 2 cut(s) 396, 428
BpuEI CTTGAG 1 cut(s) 356
BpuMI CCSGG 1 cut(s) 81
Bsa29I ATCGAT 1 cut(s) 144
BsaJI CCNNGG 1 cut(s) 272
BsaXI ACNNNNNCTCC 2 cut(s) 438, 468
Bsc4I CCNNNNNNNGG 1 cut(s) 80
Bse1I ACTGG 1 cut(s) 537
Bse3DI GCAATG 2 cut(s) 228, 429
BseBI CCWGG 2 cut(s) 20, 285
BseCI ATCGAT 1 cut(s) 144
BseDI CCNNGG 1 cut(s) 272
BseLI CCNNNNNNNGG 1 cut(s) 80
BseMI GCAATG 2 cut(s) 228, 429
BseMII CTCAG 1 cut(s) 254
BseNI ACTGG 1 cut(s) 537
BseXI GCAGC 1 cut(s) 406
Bsh1236I CGCG 1 cut(s) 30
BshVI ATCGAT 1 cut(s) 144
BsiHKAI GWGCWC 1 cut(s) 376
BsiHKCI CYCGRG 1 cut(s) 204
BsiSI CCGG 1 cut(s) 81
BslFI GGGAC 3 cut(s) 47, 341, 526
BslI CCNNNNNNNGG 1 cut(s) 80
BsmFI GGGAC 3 cut(s) 47, 341, 526
BsoBI CYCGRG 1 cut(s) 204
Bsp1286I GDGCHC 1 cut(s) 376
Bsp143I GATC 2 cut(s) 22, 367
BspCNI CTCAG 1 cut(s) 255
BspDI ATCGAT 1 cut(s) 144
BspFNI CGCG 1 cut(s) 30
BspLI GGNNCC 2 cut(s) 24, 329
BspPI GGATC 2 cut(s) 17, 30
BsrDI GCAATG 2 cut(s) 228, 429
BsrI ACTGG 1 cut(s) 537
BssECI CCNNGG 1 cut(s) 272
BssMI GATC 2 cut(s) 22, 367
BssT1I CCWWGG 1 cut(s) 272
Bst2UI CCWGG 2 cut(s) 20, 285
Bst4CI ACNGT 1 cut(s) 332
Bst6I CTCTTC 1 cut(s) 297
BstC8I GCNNGC 2 cut(s) 410, 469
BstDEI CTNAG 1 cut(s) 263
BstFNI CGCG 1 cut(s) 30
BstKTI GATC 2 cut(s) 25, 370
BstMBI GATC 2 cut(s) 22, 367
BstMWI GCNNNNNNNGC 1 cut(s) 245
BstNI CCWGG 2 cut(s) 20, 285
BstNSI RCATGY 1 cut(s) 347
BstSCI CCNGG 3 cut(s) 18, 79, 283
BstUI CGCG 1 cut(s) 30
BstV1I GCAGC 1 cut(s) 406
BstX2I RGATCY 1 cut(s) 22
BstXI CCANNNNNNTGG 1 cut(s) 235
BstYI RGATCY 1 cut(s) 22
Bsu15I ATCGAT 1 cut(s) 144
BsuTUI ATCGAT 1 cut(s) 144
BtsIMutI CAGTG 1 cut(s) 189
Cac8I GCNNGC 2 cut(s) 410, 469
Cfr13I GGNCC 2 cut(s) 221, 328
ClaI ATCGAT 1 cut(s) 144
CseI GACGC 1 cut(s) 19
Csp6I GTAC 2 cut(s) 307, 530
CviAII CATG 2 cut(s) 35, 344
CviJI RGCY 4 cut(s) 8, 128, 203, 398
CviKI_1 RGCY 4 cut(s) 8, 128, 203, 398
CviQI GTAC 2 cut(s) 307, 530
DdeI CTNAG 1 cut(s) 263
DpnI GATC 2 cut(s) 24, 369
DpnII GATC 2 cut(s) 22, 367
Eam1104I CTCTTC 1 cut(s) 297
EarI CTCTTC 1 cut(s) 297
Eco130I CCWWGG 1 cut(s) 272
Eco32I GATATC 1 cut(s) 100
Eco47I GGWCC 2 cut(s) 221, 328
Eco88I CYCGRG 1 cut(s) 204
EcoRII CCWGG 2 cut(s) 18, 283
EcoRV GATATC 1 cut(s) 100
EcoT14I CCWWGG 1 cut(s) 272
EcoT22I ATGCAT 1 cut(s) 345
ErhI CCWWGG 1 cut(s) 272
FaeI CATG 2 cut(s) 38, 347
FaiI YATR 6 cut(s) 36, 89, 149, 321, 345, 528
FaqI GGGAC 3 cut(s) 47, 341, 526
FatI CATG 2 cut(s) 34, 343
Fnu4HI GCNGC 1 cut(s) 420
Fsp4HI GCNGC 1 cut(s) 420
FspBI CTAG 1 cut(s) 116
GluI GCNGC 1 cut(s) 420
HapII CCGG 1 cut(s) 81
HgaI GACGC 1 cut(s) 19
Hin1II CATG 2 cut(s) 38, 347
HinfI GANTC 3 cut(s) 52, 57, 539
HpaII CCGG 1 cut(s) 81
HphI GGTGA 2 cut(s) 299, 377
Hpy166II GTNNAC 1 cut(s) 309
Hpy188III TCNNGA 2 cut(s) 335, 500
Hpy8I GTNNAC 1 cut(s) 309
HpyAV CCTTC 2 cut(s) 139, 318
HpyCH4III ACNGT 1 cut(s) 332
HpyCH4IV ACGT 1 cut(s) 439
HpyCH4V TGCA 3 cut(s) 191, 343, 422
HpyF10VI GCNNNNNNNGC 1 cut(s) 245
HpyF3I CTNAG 1 cut(s) 263
HpySE526I ACGT 1 cut(s) 439
Hsp92II CATG 2 cut(s) 38, 347
Kzo9I GATC 2 cut(s) 22, 367
LmnI GCTCC 3 cut(s) 245, 350, 446
Lsp1109I GCAGC 1 cut(s) 406
LweI GCATC 1 cut(s) 330
MaeI CTAG 1 cut(s) 116
MaeII ACGT 1 cut(s) 439
MalI GATC 2 cut(s) 24, 369
MboI GATC 2 cut(s) 22, 367
MboII GAAGA 1 cut(s) 314
MfeI CAATTG 1 cut(s) 240
MflI RGATCY 1 cut(s) 22
MhlI GDGCHC 1 cut(s) 376
MluCI AATT 5 cut(s) 67, 84, 195, 240, 278
MlyI GAGTC 2 cut(s) 66, 548
MmeI TCCRAC 2 cut(s) 328, 424
MnlI CCTC 5 cut(s) 36, 193, 200, 234, 398
Mph1103I ATGCAT 1 cut(s) 345
MseI TTAA 1 cut(s) 392
MslI CAYNNNNRTG 1 cut(s) 182
MspI CCGG 1 cut(s) 81
MspR9I CCNGG 3 cut(s) 20, 81, 285
MunI CAATTG 1 cut(s) 240
MvaI CCWGG 2 cut(s) 20, 285
MvnI CGCG 1 cut(s) 30
MwoI GCNNNNNNNGC 1 cut(s) 245
NciI CCSGG 1 cut(s) 81
NdeII GATC 2 cut(s) 22, 367
NlaIII CATG 2 cut(s) 38, 347
NlaIV GGNNCC 2 cut(s) 24, 329
NsiI ATGCAT 1 cut(s) 345
NspI RCATGY 1 cut(s) 347
PaeR7I CTCGAG 1 cut(s) 204
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PfeI GAWTC 1 cut(s) 52
PkrI GCNGC 1 cut(s) 421
PleI GAGTC 2 cut(s) 65, 547
PpsI GAGTC 2 cut(s) 65, 547
Psp6I CCWGG 2 cut(s) 18, 283
PspGI CCWGG 2 cut(s) 18, 283
PspN4I GGNNCC 2 cut(s) 24, 329
PspPI GGNCC 2 cut(s) 221, 328
PspXI VCTCGAGB 1 cut(s) 204
PsuI RGATCY 1 cut(s) 22
RsaI GTAC 2 cut(s) 308, 531
RsaNI GTAC 2 cut(s) 307, 530
RseI CAYNNNNRTG 1 cut(s) 182
SaqAI TTAA 1 cut(s) 392
SatI GCNGC 1 cut(s) 420
Sau3AI GATC 2 cut(s) 22, 367
Sau96I GGNCC 2 cut(s) 221, 328
SchI GAGTC 2 cut(s) 66, 548
ScrFI CCNGG 3 cut(s) 20, 81, 285
SduI GDGCHC 1 cut(s) 376
SetI ASST 2 cut(s) 211, 442
SfaNI GCATC 1 cut(s) 330
Sfr274I CTCGAG 1 cut(s) 204
SinI GGWCC 2 cut(s) 221, 328
SlaI CTCGAG 1 cut(s) 204
SmiMI CAYNNNNRTG 1 cut(s) 182
SmlI CTYRAG 2 cut(s) 204, 335
SmoI CTYRAG 2 cut(s) 204, 335
Sse9I AATT 5 cut(s) 67, 84, 195, 240, 278
SspMI CTAG 1 cut(s) 116
StyD4I CCNGG 3 cut(s) 18, 79, 283
StyI CCWWGG 1 cut(s) 272
TaaI ACNGT 1 cut(s) 332
TaiI ACGT 1 cut(s) 442
TaqI TCGA 3 cut(s) 55, 144, 205
TasI AATT 5 cut(s) 67, 84, 195, 240, 278
TatI WGTACW 2 cut(s) 306, 529
TfiI GAWTC 1 cut(s) 52
Tru1I TTAA 1 cut(s) 392
Tru9I TTAA 1 cut(s) 392
TscAI CASTG 1 cut(s) 189
TseI GCWGC 1 cut(s) 419
TspDTI ATGAA 1 cut(s) 202
TspRI CASTG 1 cut(s) 189
VpaK11BI GGWCC 2 cut(s) 221, 328
XceI RCATGY 1 cut(s) 347
XhoI CTCGAG 1 cut(s) 204
XspI CTAG 1 cut(s) 116
Zsp2I ATGCAT 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.