Homeodomain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 339 |
| AccB1I | GGYRCC | 2 cut(s) | 282, 712 |
| AciI | CCGC | 4 cut(s) | 99, 286, 357, 543 |
| AcoI | YGGCCR | 2 cut(s) | 100, 649 |
| AcuI | CTGAAG | 1 cut(s) | 436 |
| AcyI | GRCGYC | 1 cut(s) | 713 |
| AfaI | GTAC | 1 cut(s) | 462 |
| AgsI | TTSAA | 3 cut(s) | 52, 223, 517 |
| AjnI | CCWGG | 2 cut(s) | 289, 519 |
| AluBI | AGCT | 7 cut(s) | 8, 26, 35, 215, 237, 702, 774 |
| AluI | AGCT | 7 cut(s) | 8, 26, 35, 215, 237, 702, 774 |
| Alw21I | GWGCWC | 1 cut(s) | 212 |
| Alw26I | GTCTC | 1 cut(s) | 24 |
| Ama87I | CYCGRG | 1 cut(s) | 274 |
| AoxI | GGCC | 4 cut(s) | 100, 342, 616, 649 |
| ApeKI | GCWGC | 2 cut(s) | 5, 200 |
| AspLEI | GCGC | 2 cut(s) | 340, 715 |
| AspS9I | GGNCC | 2 cut(s) | 579, 616 |
| AsuHPI | GGTGA | 4 cut(s) | 137, 484, 523, 664 |
| AvaI | CYCGRG | 1 cut(s) | 274 |
| AvaII | GGWCC | 1 cut(s) | 579 |
| AxyI | CCTNAGG | 1 cut(s) | 133 |
| BanI | GGYRCC | 2 cut(s) | 282, 712 |
| Bbv12I | GWGCWC | 1 cut(s) | 212 |
| BbvCI | CCTCAGC | 1 cut(s) | 765 |
| BbvI | GCAGC | 2 cut(s) | 17, 212 |
| BccI | CCATC | 2 cut(s) | 221, 503 |
| BceAI | ACGGC | 2 cut(s) | 112, 664 |
| BciT130I | CCWGG | 2 cut(s) | 291, 521 |
| BclI | TGATCA | 1 cut(s) | 528 |
| BcoDI | GTCTC | 1 cut(s) | 24 |
| BfaI | CTAG | 2 cut(s) | 23, 32 |
| BfmI | CTRYAG | 1 cut(s) | 198 |
| BfoI | RGCGCY | 1 cut(s) | 716 |
| BisI | GCNGC | 4 cut(s) | 6, 100, 201, 286 |
| BlpI | GCTNAGC | 1 cut(s) | 396 |
| BlsI | GCNGC | 4 cut(s) | 7, 101, 202, 287 |
| Bme1390I | CCNGG | 2 cut(s) | 291, 521 |
| Bme18I | GGWCC | 1 cut(s) | 579 |
| BmeT110I | CYCGRG | 1 cut(s) | 274 |
| BmgT120I | GGNCC | 2 cut(s) | 579, 616 |
| BmiI | GGNNCC | 5 cut(s) | 190, 284, 580, 617, 714 |
| BmrFI | CCNGG | 2 cut(s) | 291, 521 |
| BmrI | ACTGGG | 1 cut(s) | 622 |
| BmsI | GCATC | 2 cut(s) | 118, 306 |
| BmuI | ACTGGG | 1 cut(s) | 622 |
| BpmI | CTGGAG | 1 cut(s) | 362 |
| Bpu10I | CCTNAGC | 1 cut(s) | 765 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 396 |
| BpuEI | CTTGAG | 2 cut(s) | 253, 743 |
| BsaHI | GRCGYC | 1 cut(s) | 713 |
| BsaJI | CCNNGG | 3 cut(s) | 178, 275, 289 |
| Bse1I | ACTGG | 4 cut(s) | 592, 617, 629, 678 |
| Bse21I | CCTNAGG | 1 cut(s) | 133 |
| BseBI | CCWGG | 2 cut(s) | 291, 521 |
| BseDI | CCNNGG | 3 cut(s) | 178, 275, 289 |
| BseMII | CTCAG | 4 cut(s) | 147, 197, 387, 756 |
| BseNI | ACTGG | 4 cut(s) | 592, 617, 629, 678 |
| BseRI | GAGGAG | 2 cut(s) | 18, 162 |
| BseXI | GCAGC | 2 cut(s) | 17, 212 |
| BsgI | GTGCAG | 1 cut(s) | 567 |
| BshFI | GGCC | 4 cut(s) | 102, 344, 618, 651 |
| BshNI | GGYRCC | 2 cut(s) | 282, 712 |
| BsiHKAI | GWGCWC | 1 cut(s) | 212 |
| BsiHKCI | CYCGRG | 1 cut(s) | 274 |
| BsiSI | CCGG | 2 cut(s) | 432, 739 |
| BslFI | GGGAC | 1 cut(s) | 566 |
| BsmAI | GTCTC | 1 cut(s) | 24 |
| BsmFI | GGGAC | 1 cut(s) | 566 |
| BsnI | GGCC | 4 cut(s) | 102, 344, 618, 651 |
| BsoBI | CYCGRG | 1 cut(s) | 274 |
| Bsp1286I | GDGCHC | 1 cut(s) | 212 |
| Bsp143I | GATC | 2 cut(s) | 528, 570 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 396 |
| BspACI | CCGC | 4 cut(s) | 99, 286, 357, 543 |
| BspANI | GGCC | 4 cut(s) | 102, 344, 618, 651 |
| BspCNI | CTCAG | 4 cut(s) | 146, 198, 388, 757 |
| BspLI | GGNNCC | 5 cut(s) | 190, 284, 580, 617, 714 |
| BspMAI | CTGCAG | 1 cut(s) | 202 |
| BspQI | GCTCTTC | 1 cut(s) | 42 |
| BspT107I | GGYRCC | 2 cut(s) | 282, 712 |
| BsrI | ACTGG | 4 cut(s) | 592, 617, 629, 678 |
| BssECI | CCNNGG | 3 cut(s) | 178, 275, 289 |
| BssMI | GATC | 2 cut(s) | 528, 570 |
| BssNI | GRCGYC | 1 cut(s) | 713 |
| BssT1I | CCWWGG | 1 cut(s) | 178 |
| Bst2UI | CCWGG | 2 cut(s) | 291, 521 |
| Bst4CI | ACNGT | 3 cut(s) | 428, 686, 694 |
| Bst6I | CTCTTC | 3 cut(s) | 42, 53, 451 |
| BstACI | GRCGYC | 1 cut(s) | 713 |
| BstC8I | GCNNGC | 2 cut(s) | 342, 402 |
| BstDEI | CTNAG | 4 cut(s) | 133, 206, 396, 765 |
| BstH2I | RGCGCY | 1 cut(s) | 716 |
| BstHHI | GCGC | 2 cut(s) | 340, 715 |
| BstKTI | GATC | 2 cut(s) | 531, 573 |
| BstMAI | GTCTC | 1 cut(s) | 24 |
| BstMBI | GATC | 2 cut(s) | 528, 570 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 11, 32, 294, 363, 401 |
| BstNI | CCWGG | 2 cut(s) | 291, 521 |
| BstNSI | RCATGY | 1 cut(s) | 156 |
| BstSCI | CCNGG | 2 cut(s) | 289, 519 |
| BstSFI | CTRYAG | 1 cut(s) | 198 |
| BstV1I | GCAGC | 2 cut(s) | 17, 212 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 185 |
| Bsu36I | CCTNAGG | 1 cut(s) | 133 |
| BsuRI | GGCC | 4 cut(s) | 102, 344, 618, 651 |
| BtsI | GCAGTG | 1 cut(s) | 195 |
| BtsIMutI | CAGTG | 3 cut(s) | 195, 203, 685 |
| Cac8I | GCNNGC | 2 cut(s) | 342, 402 |
| CfoI | GCGC | 2 cut(s) | 340, 715 |
| Cfr13I | GGNCC | 2 cut(s) | 579, 616 |
| CsiI | ACCWGGT | 1 cut(s) | 519 |
| Csp6I | GTAC | 1 cut(s) | 461 |
| CviAII | CATG | 3 cut(s) | 153, 465, 601 |
| CviQI | GTAC | 1 cut(s) | 461 |
| DdeI | CTNAG | 4 cut(s) | 133, 206, 396, 765 |
| DinI | GGCGCC | 1 cut(s) | 714 |
| DpnI | GATC | 2 cut(s) | 530, 572 |
| DpnII | GATC | 2 cut(s) | 528, 570 |
| EaeI | YGGCCR | 2 cut(s) | 100, 649 |
| Eam1104I | CTCTTC | 3 cut(s) | 42, 53, 451 |
| EarI | CTCTTC | 3 cut(s) | 42, 53, 451 |
| Eco130I | CCWWGG | 1 cut(s) | 178 |
| Eco47I | GGWCC | 1 cut(s) | 579 |
| Eco57I | CTGAAG | 1 cut(s) | 436 |
| Eco81I | CCTNAGG | 1 cut(s) | 133 |
| Eco88I | CYCGRG | 1 cut(s) | 274 |
| EcoRII | CCWGG | 2 cut(s) | 289, 519 |
| EcoT14I | CCWWGG | 1 cut(s) | 178 |
| EgeI | GGCGCC | 1 cut(s) | 714 |
| EheI | GGCGCC | 1 cut(s) | 714 |
| ErhI | CCWWGG | 1 cut(s) | 178 |
| FaeI | CATG | 3 cut(s) | 156, 468, 604 |
| FaqI | GGGAC | 1 cut(s) | 566 |
| FatI | CATG | 3 cut(s) | 152, 464, 600 |
| FbaI | TGATCA | 1 cut(s) | 528 |
| Fnu4HI | GCNGC | 4 cut(s) | 6, 100, 201, 286 |
| Fsp4HI | GCNGC | 4 cut(s) | 6, 100, 201, 286 |
| FspBI | CTAG | 2 cut(s) | 23, 32 |
| FspI | TGCGCA | 1 cut(s) | 339 |
| GlaI | GCGC | 2 cut(s) | 339, 714 |
| GluI | GCNGC | 4 cut(s) | 6, 100, 201, 286 |
| GsuI | CTGGAG | 1 cut(s) | 362 |
| HaeII | RGCGCY | 1 cut(s) | 716 |
| HaeIII | GGCC | 4 cut(s) | 102, 344, 618, 651 |
| HapII | CCGG | 2 cut(s) | 432, 739 |
| HhaI | GCGC | 2 cut(s) | 340, 715 |
| Hin1I | GRCGYC | 1 cut(s) | 713 |
| Hin1II | CATG | 3 cut(s) | 156, 468, 604 |
| Hin6I | GCGC | 2 cut(s) | 338, 713 |
| HinP1I | GCGC | 2 cut(s) | 338, 713 |
| HpaII | CCGG | 2 cut(s) | 432, 739 |
| HphI | GGTGA | 4 cut(s) | 137, 484, 523, 664 |
| Hpy166II | GTNNAC | 1 cut(s) | 492 |
| Hpy188I | TCNGA | 2 cut(s) | 455, 528 |
| Hpy188III | TCNNGA | 3 cut(s) | 582, 606, 722 |
| Hpy8I | GTNNAC | 1 cut(s) | 492 |
| Hpy99I | CGWCG | 1 cut(s) | 542 |
| HpyAV | CCTTC | 2 cut(s) | 460, 673 |
| HpyCH4III | ACNGT | 3 cut(s) | 428, 686, 694 |
| HpyCH4IV | ACGT | 3 cut(s) | 537, 555, 708 |
| HpyCH4V | TGCA | 4 cut(s) | 200, 366, 412, 548 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 11, 32, 294, 363, 401 |
| HpyF3I | CTNAG | 4 cut(s) | 133, 206, 396, 765 |
| HpySE526I | ACGT | 3 cut(s) | 537, 555, 708 |
| Hsp92I | GRCGYC | 1 cut(s) | 713 |
| Hsp92II | CATG | 3 cut(s) | 156, 468, 604 |
| HspAI | GCGC | 2 cut(s) | 338, 713 |
| KasI | GGCGCC | 1 cut(s) | 712 |
| Ksp22I | TGATCA | 1 cut(s) | 528 |
| Kzo9I | GATC | 2 cut(s) | 528, 570 |
| LguI | GCTCTTC | 1 cut(s) | 42 |
| LmnI | GCTCC | 2 cut(s) | 31, 149 |
| Lsp1109I | GCAGC | 2 cut(s) | 17, 212 |
| LweI | GCATC | 2 cut(s) | 118, 306 |
| MabI | ACCWGGT | 1 cut(s) | 519 |
| MaeI | CTAG | 2 cut(s) | 23, 32 |
| MaeII | ACGT | 3 cut(s) | 537, 555, 708 |
| MaeIII | GTNAC | 6 cut(s) | 263, 304, 503, 670, 680, 694 |
| MalI | GATC | 2 cut(s) | 530, 572 |
| MboI | GATC | 2 cut(s) | 528, 570 |
| MboII | GAAGA | 4 cut(s) | 29, 40, 468, 484 |
| MhlI | GDGCHC | 1 cut(s) | 212 |
| MluCI | AATT | 1 cut(s) | 435 |
| Mly113I | GGCGCC | 1 cut(s) | 713 |
| MmeI | TCCRAC | 1 cut(s) | 71 |
| MslI | CAYNNNNRTG | 1 cut(s) | 302 |
| MspI | CCGG | 2 cut(s) | 432, 739 |
| MspR9I | CCNGG | 2 cut(s) | 291, 521 |
| MvaI | CCWGG | 2 cut(s) | 291, 521 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 11, 32, 294, 363, 401 |
| NarI | GGCGCC | 1 cut(s) | 713 |
| NdeII | GATC | 2 cut(s) | 528, 570 |
| NlaIII | CATG | 3 cut(s) | 156, 468, 604 |
| NlaIV | GGNNCC | 5 cut(s) | 190, 284, 580, 617, 714 |
| NmuCI | GTSAC | 6 cut(s) | 263, 304, 503, 670, 680, 694 |
| NsbI | TGCGCA | 1 cut(s) | 339 |
| NspI | RCATGY | 1 cut(s) | 156 |
| PciSI | GCTCTTC | 1 cut(s) | 42 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 690 |
| PkrI | GCNGC | 4 cut(s) | 7, 101, 202, 287 |
| PluTI | GGCGCC | 1 cut(s) | 716 |
| Psp6I | CCWGG | 2 cut(s) | 289, 519 |
| PspGI | CCWGG | 2 cut(s) | 289, 519 |
| PspN4I | GGNNCC | 5 cut(s) | 190, 284, 580, 617, 714 |
| PspPI | GGNCC | 2 cut(s) | 579, 616 |
| PstI | CTGCAG | 1 cut(s) | 202 |
| RsaI | GTAC | 1 cut(s) | 462 |
| RsaNI | GTAC | 1 cut(s) | 461 |
| RseI | CAYNNNNRTG | 1 cut(s) | 302 |
| SapI | GCTCTTC | 1 cut(s) | 42 |
| SatI | GCNGC | 4 cut(s) | 6, 100, 201, 286 |
| Sau3AI | GATC | 2 cut(s) | 528, 570 |
| Sau96I | GGNCC | 2 cut(s) | 579, 616 |
| ScrFI | CCNGG | 2 cut(s) | 291, 521 |
| SduI | GDGCHC | 1 cut(s) | 212 |
| SexAI | ACCWGGT | 1 cut(s) | 519 |
| SfaNI | GCATC | 2 cut(s) | 118, 306 |
| SfcI | CTRYAG | 1 cut(s) | 198 |
| SfoI | GGCGCC | 1 cut(s) | 714 |
| SinI | GGWCC | 1 cut(s) | 579 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 302 |
| SmlI | CTYRAG | 2 cut(s) | 232, 722 |
| SmoI | CTYRAG | 2 cut(s) | 232, 722 |
| Sse9I | AATT | 1 cut(s) | 435 |
| SsiI | CCGC | 4 cut(s) | 99, 286, 357, 543 |
| SspDI | GGCGCC | 1 cut(s) | 712 |
| SspMI | CTAG | 2 cut(s) | 23, 32 |
| StyD4I | CCNGG | 2 cut(s) | 289, 519 |
| StyI | CCWWGG | 1 cut(s) | 178 |
| TaaI | ACNGT | 3 cut(s) | 428, 686, 694 |
| TaiI | ACGT | 3 cut(s) | 540, 558, 711 |
| TaqI | TCGA | 2 cut(s) | 387, 569 |
| TasI | AATT | 1 cut(s) | 435 |
| TauI | GCSGC | 2 cut(s) | 102, 288 |
| TscAI | CASTG | 3 cut(s) | 202, 210, 685 |
| TseFI | GTSAC | 6 cut(s) | 263, 304, 503, 670, 680, 694 |
| TseI | GCWGC | 2 cut(s) | 5, 200 |
| Tsp45I | GTSAC | 6 cut(s) | 263, 304, 503, 670, 680, 694 |
| TspDTI | ATGAA | 2 cut(s) | 267, 548 |
| TspRI | CASTG | 3 cut(s) | 202, 210, 685 |
| VpaK11BI | GGWCC | 1 cut(s) | 579 |
| XceI | RCATGY | 1 cut(s) | 156 |
| XspI | CTAG | 2 cut(s) | 23, 32 |