Rorug04G0104400

Sieve element occlusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000322
Physical Location & Seq
Reverse (-)
16171846 .. 16180123
8278 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0104400.1

Sequence Viewer

Length: 3573 bp
ATGGCTGCCATTGATGATGGTTTGAAGAAGCTCGAGTACCTTTCCTTGGTTTCCAAAGTCTGTTCAGAGCTGGAAACCCATCTAGGGTTTGGGGATAAAGTTCTTGCTGAGTTCATAACTGAGATGGGACGAAACTGTGGGAACGTGGATGAATTTGATGCCAAATTGAAGGAGAACGGTGCCGAGATGCCCGATTACTTTGTCCGCACACTCCTCACTATCATTCATGCAATTCTTCCTCCGAAGCCAAAGTCTGAGAATGACTCAATGATAGAGAATACTACTGAAGGTAAGAAGACCAAGTTTAAGGCTCTGTCTATTGCTGATAACAGGGATAGGGTAAATGATCTCCAGAGGGAAATTGAGATGGAGACCAAGGAAAAGAAAAATGACCGAGAAGAGTGGGGTGATGAGCGTGAAGAAGATAGGCGTAGAGGTAGAGGTAGAGATAGAGACAGATATGGGGAAAGAGATCGAGACAGAGATAGAGATAGGGATAGGAGAGAGAGGGGCAGGGATAGGTATGACAGAGATGAGAGGCGAAAAGATACACATCATGATTATGATAAGGGAAGGCATACAGACCGATCCAATAAGCACAGAAGGGATGGGTATCAAGAGGATGAAGATGTTAGAGAGGAGGGTGGTAACAGGAGAGGAGGTGGGGATCAGCGGAGTGGACTGCACCATTCTGATGAGCCTGAATTGTATCAGGTTTATAAGGGCAGGGTATCAAAAGTCATGGACACGGGTTGCTTTGTTCAGTTCAGTGACTTTAGGGGGAAGGAGGGTCTGGTTCATGTTTCACAGATGGCAACGCGGCGTATTGGTAATGCTAAAGATGTGGTGAAGAGGGATCAAGAGGTCTATGTTAAGGTGATTTCAGTTTCTGGTCAGAAGTTGAGCCTTTCAATGAGGGATGTTGATCAGCATACCGGAAAGGATTTGCTTCCATTGAAGAAGAGCTCAGAGGATGATGCTCATAGGACAAACCCTTCGGTATCAAAGCATGATGGGCCTGTGACAAGGACTGGTCTTTCTGGGATTAGAATTGTGGAAGAGAACAATGTTGCCCCTTCTCGGCGGCCATTAAAGAGAATGAGCTCGCCGGAGAAGTGGGAAGCCAAACAGTTGATTGCCTCAGGTGTTTTGGGTGTTACAGAATATCCAATGTATGATGAGGAAACAGAGGGGATGCTTTATGAAGAAGAGGGAGCTGAGGAAGAGCTTGAAATTGAGCTTAATGAGGATGAGCCAGCCTTTTTGCAAGGGCAAAGCAGGTATTCTGTAGATATGTCACCTGTGAAAATCTTTAAGAATCCAGAGGGGTCATTAAGTCGTGCAGCTGCACTTCAATCTGCACTTATTAAAGAGCGCAGAGAAGTGCGTGAGCAGCAGCAAAGAACCATGTTGGATTCAATCCCAAAGGATCTGAATCGTCCTTGGGAAGATCCAATGCCAGAGACTGGTGAGAGGCATCTTGCACAGGAGCTTAGAGGTGTTGGTTTGTCGGCCTATGACATGCCTGAGTGGAAGAAGGATGCTTTTGGGAAGACTGTCAGTTTTGGGCAGAGGTCAAAGCTCTCACTTCAGGAACAGAGGCAGAGCTTGCCCATCTACAAGCTCAAGAAAGAATTGGTTCAGGCAGTGCATGAAAATCAAGTTCTTGTTGTCATTGGTGAGACTGGTTCGGGCAAGACAACCCAGGTAACACAATATCTTGCAGAAGCAGGTTACACAACAATGGGTAAGATTGGGTGTACACAACCACGTAGGGTGGCCGCGATGTCTGTAGCCAAGAGAGTTGCTGAAGAATTTGGTTGTCGTTTGGGGGAGGAAGTTGGCTATGCTATTCGTTTTGAGGATTGCACTGGCCCTGATACTGTCATCAAGTACATGACTGATGGTATGCTTCTTAGGGAGATTTTGATCGATGAAAACCTTTCTCAGTACTCTGTGGTCATGCTTGATGAAGCTCATGAGAGGACAATCCATACAGATGTTCTTTTTGGATTATTGAAGAAACTTATCAAGCGGAGACCAGACCTACGTTTGATTGTCACATCTGCTACCTTAGATGCTGAGAAGTTTTCAGGTTATTTCTTCGACTGTAACATCTTCACTATACCAGGGAGAACTTTTCCTGTAGAGATACTATACACCAAGCAGCCAGAAAGTGATTATCTTGATGCATCTCTAATCACTGTTCTACAGATTCACTTAACAGAACCAGAAGGGGACATCCTTCTCTTCTTGACGGGACAGGAAGAAATTGATTTCTCATGCCAGTCACTTTTTGAGAGGATGAAAGGTCTTGGTAAAAATGTACCTGAGTTGATTATCTTACCAGTTTACAGTGCCCTTCCTAGTGAAATGCAGTCTAGGATTTTTGATCCAGCTCCACCTGGGAAGAGGAAAGTAGTTGTGGCTACTAATATTGCTGAGGCATCACTAACCATTGATGGGATATTTTATGTAATTGATCCTGGATTTGCAAAGCAAAATGTTTATAACCCAAAGCAAGGGCTGGATTCACTGGTCATAACCCCAATTTCACAAGCATCAGCCAAGCAAAGAGCTGGGCGCGCTGGGCGTACTGGGCCTGGAAAGTGTTACCGCCTCTACACTGAGAGTGCATACCGCAACGAGATGTCCCCTACTTCAGTTCCAGAAATTCAGAGGATAAATCTTGGGTTAACTACACTTACCATGAAAGCAATGGGGATAAACGATCTGTTGTCTTTTGATTTTATGGATCCTCCCTCACCCCAAGCACTCATTTCTGCTATGGAACAACTGTACAGTTTAGGAGCACTCGACGAGGAGGGGCTTCTCACCAAACTGGGTAGGAAAATGGCTGAGTTTCCTCTTGATCCACCACTATCTAAGATGCTACTGGCCAGTGTGGACCTTGGATGCAGTGATGAGATCTTGACCATGATTGCCATGATTCAGACAGGCAATATATTTTACAGGCCTAGGGAAAAACAAGCCCAGGCTGATCAGAAGAGAGCCAAGTTTTTCCAGCCAGAGGGAGACCATCTGACTTTACTTGCAGTGTATGAGGCTTGGAAAGCTAAGAACTTTTCAGGGCCATGGTGCTTTGAGAACTTCGTTCAGTCTCGATCCCTTAGGAGGGCACAGGATGTCAGAAAACAGCTTCTAAGCATCATGGATAAGTATAAACTGGATGTTGTGAGTGCTGGAAAGAATTTTACACAGATCAGGAAGGCAATTACGGCGGGATTTTTCTTCCATGGTGCTAGAAAGGACCCTCAGGAGGGGTATAGAACCTTAGTGGAGAACCAGCCAGTTTATATCCATCCAAGCAGTGCGCTCTTCCAGAGACAACCAGATTGGATCATCTACCATGAGTTGGTTATGACTACGAAGGAGTATATGCGTGAGGTAACCGTCGTTGACCCCAAATGGCTTGTGGAACTAGCACCCAGGTTCTTCAAAGTGGCAGACCCTACCAAGATGAGTAAGCGCAAGCGTCAAGAGCGTATTGAACCGCTATATGACAGATACCATGAACCAAACTCTTGGCGTCTTAGTAAACGACGTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEO_C SEO_N

Protein Analysis

1190

Amino Acids

135.97

Weight (kDa)

6.35

Isoelectric Point (pI)

48.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 235 - 305 1.3e-11 S1 RNA binding domain
DEAD PF00270 544 - 693 1.2e-07 DEAD/DEAH box helicase
AAA_22 PF13401 552 - 677 2.9e-06 AAA domain
Helicase_C PF00271 737 - 867 8.8e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 929 - 957 8.2e-11 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 958 - 1017 3e-14 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1075 - 1151 8.4e-26 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000322)

Species Orthologous Gene IDs
rosa_rugosa Rorug01G0045800 Rorug01G0060200 Rorug01G0078200 Rorug01G0080300 Rorug01G0080500 Rorug01G0093600 Rorug01G0096100 Rorug01G0138500.1 Rorug01G0147900.1 Rorug01G0156300.1 Rorug01G0181600 Rorug01G0183600 Rorug01G0183600 Rorug01G0196000 Rorug01G0206200 Rorug01G0206200 Rorug01G0206300 Rorug01G0206400 Rorug01G0245700 Rorug01G0267300 Rorug01G0268000 Rorug01G0324700 Rorug01G0324800 Rorug02G0034400 Rorug02G0034500 Rorug02G0104900 Rorug02G0224800 Rorug02G0224900 Rorug02G0247400 Rorug02G0261500 Rorug02G0288300 Rorug02G0297300 Rorug02G0297400 Rorug02G0314900 Rorug02G0362700 Rorug02G0438500 Rorug02G0438600 Rorug02G0438700 Rorug02G0438800 Rorug02G0525300 Rorug02G0540100 Rorug03G0147100 Rorug03G0150900 Rorug03G0171600 Rorug03G0182000 Rorug03G0200200 Rorug03G0201100 Rorug03G0218400 Rorug03G0218700 Rorug03G0268200 Rorug03G0285500 Rorug03G0296600 Rorug03G0296700 Rorug03G0296800 Rorug03G0341800 Rorug03G0347200 Rorug03G0356300 Rorug04G0029100 Rorug04G0036300 Rorug04G0046500 Rorug04G0070100 Rorug04G0098900 Rorug04G0104400 Rorug04G0167800 Rorug04G0209300 Rorug04G0210700 Rorug04G0210800 Rorug04G0212500 Rorug05G0109400 Rorug05G0148500 Rorug05G0148800 Rorug05G0160200 Rorug05G0184600 Rorug05G0206100 Rorug05G0208500 Rorug05G0208500 Rorug05G0209500 Rorug05G0235800 Rorug05G0239200 Rorug05G0301100 Rorug05G0427000 Rorug05G0451700 Rorug05G0462300 Rorug05G0467300 Rorug05G0524100 Rorug05G0527400 Rorug05G0527500 Rorug05G0561800 Rorug05G0567700 Rorug05G0574200 Rorug06G0000900 Rorug06G0018700.1 Rorug06G0025100 Rorug06G0025200 Rorug06G0025500 Rorug06G0041300 Rorug06G0048100 Rorug06G0050800 Rorug06G0053900 Rorug06G0075200 Rorug06G0080600 Rorug06G0087500.1 Rorug06G0090000 Rorug06G0141200 Rorug06G0218300 Rorug07G0106700 Rorug07G0125500 Rorug07G0185500 Rorug07G0194000 Rorug07G0196600 Rorug07G0200600 Rorug07G0217300 Rorug07G0250300 Rorug07G0301900 Rorug07G0309100.1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 720, 2511
Acc36I ACCTGC 2 cut(s) 1269, 1721
AccB1I GGYRCC 1 cut(s) 179
AccB7I CCANNNNNTGG 2 cut(s) 1466, 3376
AccII CGCG 3 cut(s) 820, 1784, 2586
AcoI YGGCCR 3 cut(s) 1085, 1779, 2898
AcsI RAATTY 4 cut(s) 152, 1814, 2673, 3211
AcuI CTGAAG 4 cut(s) 306, 1574, 1830, 2646
AcyI GRCGYC 1 cut(s) 3550
AfaI GTAC 7 cut(s) 38, 1762, 1895, 1952, 2328, 2596, 2801
AjiI CACGTC 1 cut(s) 3566
AjnI CCWGG 7 cut(s) 1704, 2128, 2404, 2485, 2602, 2994, 3449
Alw21I GWGCWC 3 cut(s) 968, 1106, 2815
Alw26I GTCTC 9 cut(s) 365, 447, 471, 1457, 1676, 2032, 3030, 3126, 3340
AlwNI CAGNNNCTG 2 cut(s) 890, 1466
Ama87I CYCGRG 1 cut(s) 32
AoxI GGCC 9 cut(s) 1016, 1085, 1512, 1779, 1873, 2600, 2898, 2975, 3092
ApeKI GCWGC 6 cut(s) 5, 1343, 1346, 1393, 1396, 2167
ApoI RAATTY 4 cut(s) 152, 1814, 2673, 3211
ArsI GACNNNNNNTTYG 4 cut(s) 236, 268, 979, 1011
Asp700I GAANNNNTTC 1 cut(s) 1638
AspA2I CCTAGG 1 cut(s) 2978
AspLEI GCGC 5 cut(s) 1377, 2586, 2588, 3337, 3492
AspS9I GGNCC 6 cut(s) 1016, 1874, 2600, 2908, 3092, 3271
AsuHPI GGTGA 8 cut(s) 419, 859, 889, 1290, 1481, 1691, 2757, 2827
AvaI CYCGRG 1 cut(s) 32
AvaII GGWCC 2 cut(s) 2908, 3271
AvrII CCTAGG 1 cut(s) 2978
AxyI CCTNAGG 3 cut(s) 1141, 3131, 3276
BaeGI GKGCMC 2 cut(s) 2362, 3142
BalI TGGCCA 1 cut(s) 2900
BamHI GGATCC 1 cut(s) 2755
BanI GGYRCC 1 cut(s) 179
BanII GRGCYC 2 cut(s) 968, 1106
BarI GAAGNNNNNNTAC 2 cut(s) 20, 52
BbsI GAAGAC 2 cut(s) 302, 1559
Bbv12I GWGCWC 3 cut(s) 968, 1106, 2815
BbvCI CCTCAGC 2 cut(s) 1218, 2442
BbvI GCAGC 5 cut(s) 1333, 1355, 1405, 1408, 2179
BceAI ACGGC 1 cut(s) 3255
BciT130I CCWGG 7 cut(s) 1706, 2130, 2406, 2487, 2604, 2996, 3451
BclI TGATCA 2 cut(s) 925, 3001
BcoDI GTCTC 9 cut(s) 365, 447, 471, 1457, 1676, 2032, 3030, 3126, 3340
BfaI CTAG 6 cut(s) 83, 2367, 2382, 2979, 3264, 3443
BfmI CTRYAG 4 cut(s) 1287, 1791, 2145, 2210
BfuAI ACCTGC 2 cut(s) 1269, 1721
BglII AGATCT 1 cut(s) 2928
BisI GCNGC 9 cut(s) 6, 821, 1085, 1344, 1347, 1394, 1397, 1782, 2168
BlnI CCTAGG 1 cut(s) 2978
BlsI GCNGC 9 cut(s) 7, 822, 1086, 1345, 1348, 1395, 1398, 1783, 2169
BmcAI AGTACT 1 cut(s) 1952
Bme1390I CCNGG 7 cut(s) 1706, 2130, 2406, 2487, 2604, 2996, 3451
Bme18I GGWCC 2 cut(s) 2908, 3271
BmeT110I CYCGRG 1 cut(s) 32
BmgBI CACGTC 1 cut(s) 3566
BmgT120I GGNCC 6 cut(s) 1016, 1874, 2600, 2908, 3092, 3271
BmiI GGNNCC 3 cut(s) 181, 2757, 3273
BmrFI CCNGG 7 cut(s) 1706, 2130, 2406, 2487, 2604, 2996, 3451
BmrI ACTGGG 2 cut(s) 2607, 2852
BmuI ACTGGG 2 cut(s) 2607, 2852
BpiI GAAGAC 2 cut(s) 302, 1559
BplI GAGNNNNNCTC 2 cut(s) 248, 280
BpmI CTGGAG 1 cut(s) 335
Bpu10I CCTNAGC 2 cut(s) 1218, 2442
BpuEI CTTGAG 1 cut(s) 1610
Bsa29I ATCGAT 1 cut(s) 1932
BsaAI YACGTR 1 cut(s) 1772
BsaBI GATNNNNATC 5 cut(s) 552, 612, 924, 1434, 1928
BsaHI GRCGYC 1 cut(s) 3550
BsaI GGTCTC 3 cut(s) 365, 2032, 3030
BsaWI WCCGGW 1 cut(s) 935
BsaXI ACNNNNNCTCC 2 cut(s) 646, 676
Bse21I CCTNAGG 3 cut(s) 1141, 3131, 3276
Bse3DI GCAATG 1 cut(s) 2724
Bse8I GATNNNNATC 5 cut(s) 552, 612, 924, 1434, 1928
BseBI CCWGG 7 cut(s) 1706, 2130, 2406, 2487, 2604, 2996, 3451
BseCI ATCGAT 1 cut(s) 1932
BseJI GATNNNNATC 5 cut(s) 552, 612, 924, 1434, 1928
BseMI GCAATG 1 cut(s) 2724
BsePI GCGCGC 1 cut(s) 2584
BseRI GAGGAG 4 cut(s) 203, 653, 672, 2837
BseSI GKGCMC 2 cut(s) 2362, 3142
BseXI GCAGC 5 cut(s) 1333, 1355, 1405, 1408, 2179
BseYI CCCAGC 2 cut(s) 2579, 2588
BsgI GTGCAG 4 cut(s) 668, 1332, 1344, 1362
Bsh1236I CGCG 3 cut(s) 820, 1784, 2586
BshFI GGCC 9 cut(s) 1018, 1087, 1514, 1781, 1875, 2602, 2900, 2977, 3094
BshNI GGYRCC 1 cut(s) 179
BshVI ATCGAT 1 cut(s) 1932
BsiHKAI GWGCWC 3 cut(s) 968, 1106, 2815
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 2 cut(s) 936, 1109
BslFI GGGAC 4 cut(s) 141, 2252, 2274, 2638
BsmAI GTCTC 9 cut(s) 365, 447, 471, 1457, 1676, 2032, 3030, 3126, 3340
BsmFI GGGAC 4 cut(s) 141, 2252, 2274, 2638
BsnI GGCC 9 cut(s) 1018, 1087, 1514, 1781, 1875, 2602, 2900, 2977, 3094
Bso31I GGTCTC 3 cut(s) 365, 2032, 3030
BsoBI CYCGRG 1 cut(s) 32
Bsp1286I GDGCHC 5 cut(s) 968, 1106, 2362, 2815, 3142
Bsp1407I TGTACA 2 cut(s) 1760, 2799
Bsp19I CCATGG 2 cut(s) 3095, 3256
BspANI GGCC 9 cut(s) 1018, 1087, 1514, 1781, 1875, 2602, 2900, 2977, 3094
BspDI ATCGAT 1 cut(s) 1932
BspFNI CGCG 3 cut(s) 820, 1784, 2586
BspHI TCATGA 2 cut(s) 556, 1978
BspLI GGNNCC 3 cut(s) 181, 2757, 3273
BspMI ACCTGC 2 cut(s) 1269, 1721
BspQI GCTCTTC 3 cut(s) 956, 1218, 3344
BspT107I GGYRCC 1 cut(s) 179
BspTNI GGTCTC 3 cut(s) 365, 2032, 3030
BsrDI GCAATG 1 cut(s) 2724
BsrGI TGTACA 2 cut(s) 1760, 2799
BssHII GCGCGC 1 cut(s) 2584
BssNI GRCGYC 1 cut(s) 3550
BssT1I CCWWGG 7 cut(s) 45, 375, 1442, 2911, 2978, 3095, 3256
Bst2UI CCWGG 7 cut(s) 1706, 2130, 2406, 2487, 2604, 2996, 3451
BstACI GRCGYC 1 cut(s) 3550
BstAPI GCANNNNNTGC 1 cut(s) 1609
BstAUI TGTACA 2 cut(s) 1760, 2799
BstBAI YACGTR 1 cut(s) 1772
BstC8I GCNNGC 5 cut(s) 1106, 1257, 1610, 2586, 3494
BstDSI CCRYGG 2 cut(s) 3095, 3256
BstEII GGTNACC 1 cut(s) 3409
BstFNI CGCG 3 cut(s) 820, 1784, 2586
BstHHI GCGC 5 cut(s) 1377, 2586, 2588, 3337, 3492
BstMAI GTCTC 9 cut(s) 365, 447, 471, 1457, 1676, 2032, 3030, 3126, 3340
BstMWI GCNNNNNNNGC 9 cut(s) 1015, 1393, 1609, 2585, 2590, 2599, 3074, 3239, 3502
BstNI CCWGG 7 cut(s) 1706, 2130, 2406, 2487, 2604, 2996, 3451
BstNSI RCATGY 1 cut(s) 1525
BstPI GGTNACC 1 cut(s) 3409
BstSCI CCNGG 7 cut(s) 1704, 2128, 2404, 2485, 2602, 2994, 3449
BstSFI CTRYAG 4 cut(s) 1287, 1791, 2145, 2210
BstSLI GKGCMC 2 cut(s) 2362, 3142
BstUI CGCG 3 cut(s) 820, 1784, 2586
BstV1I GCAGC 5 cut(s) 1333, 1355, 1405, 1408, 2179
BstV2I GAAGAC 2 cut(s) 302, 1559
BstX2I RGATCY 4 cut(s) 1429, 1450, 2755, 2928
BstXI CCANNNNNNTGG 1 cut(s) 3546
BstYI RGATCY 4 cut(s) 1429, 1450, 2755, 2928
Bsu15I ATCGAT 1 cut(s) 1932
Bsu36I CCTNAGG 3 cut(s) 1141, 3131, 3276
BsuRI GGCC 9 cut(s) 1018, 1087, 1514, 1781, 1875, 2602, 2900, 2977, 3094
BsuTUI ATCGAT 1 cut(s) 1932
BtgI CCRYGG 2 cut(s) 3095, 3256
BtgZI GCGATG 1 cut(s) 1799
BtrI CACGTC 1 cut(s) 3566
BtsI GCAGTG 4 cut(s) 1653, 2926, 3063, 3337
BveI ACCTGC 2 cut(s) 1269, 1721
Cac8I GCNNGC 5 cut(s) 1106, 1257, 1610, 2586, 3494
CaiI CAGNNNCTG 2 cut(s) 890, 1466
CciI TCATGA 2 cut(s) 556, 1978
CfoI GCGC 5 cut(s) 1377, 2586, 2588, 3337, 3492
Cfr13I GGNCC 6 cut(s) 1016, 1874, 2600, 2908, 3092, 3271
ClaI ATCGAT 1 cut(s) 1932
CseI GACGC 2 cut(s) 3485, 3539
Csp6I GTAC 7 cut(s) 37, 1761, 1894, 1951, 2327, 2595, 2800
CviQI GTAC 7 cut(s) 37, 1761, 1894, 1951, 2327, 2595, 2800
EaeI YGGCCR 3 cut(s) 1085, 1779, 2898
Ecl136II GAGCTC 2 cut(s) 966, 1104
Eco130I CCWWGG 7 cut(s) 45, 375, 1442, 2911, 2978, 3095, 3256
Eco147I AGGCCT 1 cut(s) 2977
Eco24I GRGCYC 2 cut(s) 968, 1106
Eco31I GGTCTC 3 cut(s) 365, 2032, 3030
Eco47I GGWCC 2 cut(s) 2908, 3271
Eco53kI GAGCTC 2 cut(s) 966, 1104
Eco57I CTGAAG 4 cut(s) 306, 1574, 1830, 2646
Eco81I CCTNAGG 3 cut(s) 1141, 3131, 3276
Eco88I CYCGRG 1 cut(s) 32
Eco91I GGTNACC 1 cut(s) 3409
EcoICRI GAGCTC 2 cut(s) 966, 1104
EcoO109I RGGNCCY 1 cut(s) 3271
EcoO65I GGTNACC 1 cut(s) 3409
EcoRII CCWGG 7 cut(s) 1704, 2128, 2404, 2485, 2602, 2994, 3449
EcoT14I CCWWGG 7 cut(s) 45, 375, 1442, 2911, 2978, 3095, 3256
EcoT22I ATGCAT 1 cut(s) 2194
EcoT38I GRGCYC 2 cut(s) 968, 1106
ErhI CCWWGG 7 cut(s) 45, 375, 1442, 2911, 2978, 3095, 3256
FaqI GGGAC 4 cut(s) 141, 2252, 2274, 2638
FauI CCCGC 1 cut(s) 3235
FbaI TGATCA 2 cut(s) 925, 3001
Fnu4HI GCNGC 9 cut(s) 6, 821, 1085, 1344, 1347, 1394, 1397, 1782, 2168
FriOI GRGCYC 2 cut(s) 968, 1106
Fsp4HI GCNGC 9 cut(s) 6, 821, 1085, 1344, 1347, 1394, 1397, 1782, 2168
FspBI CTAG 6 cut(s) 83, 2367, 2382, 2979, 3264, 3443
GlaI GCGC 5 cut(s) 1376, 2585, 2587, 3336, 3491
GluI GCNGC 9 cut(s) 6, 821, 1085, 1344, 1347, 1394, 1397, 1782, 2168
GsaI CCCAGC 2 cut(s) 2583, 2592
GsuI CTGGAG 1 cut(s) 335
HaeIII GGCC 9 cut(s) 1018, 1087, 1514, 1781, 1875, 2602, 2900, 2977, 3094
HapII CCGG 2 cut(s) 936, 1109
HgaI GACGC 2 cut(s) 3485, 3539
HhaI GCGC 5 cut(s) 1377, 2586, 2588, 3337, 3492
Hin1I GRCGYC 1 cut(s) 3550
Hin6I GCGC 5 cut(s) 1375, 2584, 2586, 3335, 3490
HinP1I GCGC 5 cut(s) 1375, 2584, 2586, 3335, 3490
HincII GTYRAC 2 cut(s) 2697, 3421
HindII GTYRAC 2 cut(s) 2697, 3421
HinfI GANTC 7 cut(s) 263, 1318, 1415, 1435, 2215, 2531, 2950
HpaI GTTAAC 1 cut(s) 2697
HpaII CCGG 2 cut(s) 936, 1109
HphI GGTGA 8 cut(s) 419, 859, 889, 1290, 1481, 1691, 2757, 2827
Hpy166II GTNNAC 8 cut(s) 680, 1761, 1763, 2353, 2697, 2908, 3421, 3560
Hpy8I GTNNAC 8 cut(s) 680, 1761, 1763, 2353, 2697, 2908, 3421, 3560
Hpy99I CGWCG 3 cut(s) 2822, 3419, 3567
HpyCH4IV ACGT 4 cut(s) 144, 1771, 2050, 3565
HpyF10VI GCNNNNNNNGC 9 cut(s) 1015, 1393, 1609, 2585, 2590, 2599, 3074, 3239, 3502
HpySE526I ACGT 4 cut(s) 144, 1771, 2050, 3565
Hsp92I GRCGYC 1 cut(s) 3550
HspAI GCGC 5 cut(s) 1375, 2584, 2586, 3335, 3490
Ksp22I TGATCA 2 cut(s) 925, 3001
KspAI GTTAAC 1 cut(s) 2697
LguI GCTCTTC 3 cut(s) 956, 1218, 3344
LmnI GCTCC 4 cut(s) 1214, 1489, 2404, 2810
Lsp1109I GCAGC 5 cut(s) 1333, 1355, 1405, 1408, 2179
MaeI CTAG 6 cut(s) 83, 2367, 2382, 2979, 3264, 3443
MaeII ACGT 4 cut(s) 144, 1771, 2050, 3565
MflI RGATCY 4 cut(s) 1429, 1450, 2755, 2928
MhlI GDGCHC 5 cut(s) 968, 1106, 2362, 2815, 3142
MlsI TGGCCA 1 cut(s) 2900
MluNI TGGCCA 1 cut(s) 2900
MlyI GAGTC 1 cut(s) 257
MmeI TCCRAC 1 cut(s) 1392
Mox20I TGGCCA 1 cut(s) 2900
Mph1103I ATGCAT 1 cut(s) 2194
MroXI GAANNNNTTC 1 cut(s) 1638
MscI TGGCCA 1 cut(s) 2900
MseI TTAA 9 cut(s) 306, 873, 1091, 1242, 1314, 1334, 1368, 2222, 2696
MslI CAYNNNNRTG 4 cut(s) 561, 693, 1742, 1998
Msp20I TGGCCA 1 cut(s) 2900
MspA1I CMGCKG 2 cut(s) 673, 1346
MspI CCGG 2 cut(s) 936, 1109
MspR9I CCNGG 7 cut(s) 1706, 2130, 2406, 2487, 2604, 2996, 3451
MvaI CCWGG 7 cut(s) 1706, 2130, 2406, 2487, 2604, 2996, 3451
MvnI CGCG 3 cut(s) 820, 1784, 2586
MwoI GCNNNNNNNGC 9 cut(s) 1015, 1393, 1609, 2585, 2590, 2599, 3074, 3239, 3502
NcoI CCATGG 2 cut(s) 3095, 3256
NlaIV GGNNCC 3 cut(s) 181, 2757, 3273
NmeAIII GCCGAG 2 cut(s) 208, 1060
NmuCI GTSAC 5 cut(s) 770, 1021, 1296, 2059, 2289
NsiI ATGCAT 1 cut(s) 2194
NspI RCATGY 1 cut(s) 1525
PaeR7I CTCGAG 1 cut(s) 32
PagI TCATGA 2 cut(s) 556, 1978
PauI GCGCGC 1 cut(s) 2584
PceI AGGCCT 1 cut(s) 2977
PciSI GCTCTTC 3 cut(s) 956, 1218, 3344
PdmI GAANNNNTTC 1 cut(s) 1638
PfeI GAWTC 6 cut(s) 1318, 1415, 1435, 2215, 2531, 2950
PflMI CCANNNNNTGG 2 cut(s) 1466, 3376
PfoI TCCNGGA 1 cut(s) 2485
PkrI GCNGC 9 cut(s) 7, 822, 1086, 1345, 1348, 1395, 1398, 1783, 2169
PleI GAGTC 1 cut(s) 257
PpsI GAGTC 1 cut(s) 257
Ppu21I YACGTR 1 cut(s) 1772
PpuMI RGGWCCY 1 cut(s) 3271
PsiI TTATAA 2 cut(s) 720, 2511
Psp124BI GAGCTC 2 cut(s) 968, 1106
Psp5II RGGWCCY 1 cut(s) 3271
Psp6I CCWGG 7 cut(s) 1704, 2128, 2404, 2485, 2602, 2994, 3449
PspEI GGTNACC 1 cut(s) 3409
PspFI CCCAGC 2 cut(s) 2579, 2588
PspGI CCWGG 7 cut(s) 1704, 2128, 2404, 2485, 2602, 2994, 3449
PspN4I GGNNCC 3 cut(s) 181, 2757, 3273
PspPI GGNCC 6 cut(s) 1016, 1874, 2600, 2908, 3092, 3271
PspPPI RGGWCCY 1 cut(s) 3271
PspXI VCTCGAGB 1 cut(s) 32
PstNI CAGNNNCTG 2 cut(s) 890, 1466
PsuI RGATCY 4 cut(s) 1429, 1450, 2755, 2928
PteI GCGCGC 1 cut(s) 2584
PvuII CAGCTG 1 cut(s) 1346
RsaI GTAC 7 cut(s) 38, 1762, 1895, 1952, 2328, 2596, 2801
RsaNI GTAC 7 cut(s) 37, 1761, 1894, 1951, 2327, 2595, 2800
RseI CAYNNNNRTG 4 cut(s) 561, 693, 1742, 1998
SacI GAGCTC 2 cut(s) 968, 1106
SapI GCTCTTC 3 cut(s) 956, 1218, 3344
SaqAI TTAA 9 cut(s) 306, 873, 1091, 1242, 1314, 1334, 1368, 2222, 2696
SatI GCNGC 9 cut(s) 6, 821, 1085, 1344, 1347, 1394, 1397, 1782, 2168
Sau96I GGNCC 6 cut(s) 1016, 1874, 2600, 2908, 3092, 3271
ScaI AGTACT 1 cut(s) 1952
SchI GAGTC 1 cut(s) 257
ScrFI CCNGG 7 cut(s) 1706, 2130, 2406, 2487, 2604, 2996, 3451
SduI GDGCHC 5 cut(s) 968, 1106, 2362, 2815, 3142
SfcI CTRYAG 4 cut(s) 1287, 1791, 2145, 2210
Sfr274I CTCGAG 1 cut(s) 32
SinI GGWCC 2 cut(s) 2908, 3271
SlaI CTCGAG 1 cut(s) 32
SmiMI CAYNNNNRTG 4 cut(s) 561, 693, 1742, 1998
SmlI CTYRAG 2 cut(s) 32, 1625
SmoI CTYRAG 2 cut(s) 32, 1625
SseBI AGGCCT 1 cut(s) 2977
SspI AATATT 1 cut(s) 2437
SspMI CTAG 6 cut(s) 83, 2367, 2382, 2979, 3264, 3443
SstI GAGCTC 2 cut(s) 968, 1106
StuI AGGCCT 1 cut(s) 2977
StyD4I CCNGG 7 cut(s) 1704, 2128, 2404, 2485, 2602, 2994, 3449
StyI CCWWGG 7 cut(s) 45, 375, 1442, 2911, 2978, 3095, 3256
TaiI ACGT 4 cut(s) 147, 1774, 2053, 3568
TaqI TCGA 6 cut(s) 33, 475, 1932, 2106, 2817, 3124
TaqII GACCGA 2 cut(s) 408, 600
TatI WGTACW 4 cut(s) 1760, 1893, 1950, 2799
TauI GCSGC 3 cut(s) 823, 1087, 1784
TfiI GAWTC 6 cut(s) 1318, 1415, 1435, 2215, 2531, 2950
Tru1I TTAA 9 cut(s) 306, 873, 1091, 1242, 1314, 1334, 1368, 2222, 2696
Tru9I TTAA 9 cut(s) 306, 873, 1091, 1242, 1314, 1334, 1368, 2222, 2696
TseFI GTSAC 5 cut(s) 770, 1021, 1296, 2059, 2289
TseI GCWGC 6 cut(s) 5, 1343, 1346, 1393, 1396, 2167
Tsp45I GTSAC 5 cut(s) 770, 1021, 1296, 2059, 2289
Van91I CCANNNNNTGG 2 cut(s) 1466, 3376
VpaK11BI GGWCC 2 cut(s) 2908, 3271
XapI RAATTY 4 cut(s) 152, 1814, 2673, 3211
XceI RCATGY 1 cut(s) 1525
XcmI CCANNNNNNNNNTGG 3 cut(s) 86, 2716, 3433
XhoI CTCGAG 1 cut(s) 32
XmaJI CCTAGG 1 cut(s) 2978
XmnI GAANNNNTTC 1 cut(s) 1638
XspI CTAG 6 cut(s) 83, 2367, 2382, 2979, 3264, 3443
ZrmI AGTACT 1 cut(s) 1952
Zsp2I ATGCAT 1 cut(s) 2194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.