AT4G05150

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007930
Physical Location & Seq
Forward (+)
2660156 .. 2662908
2753 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G05150.1

Sequence Viewer

Length: 1434 bp
ATGAAATCGAATCAAAAAAAACAAAAATCCCCAAATTACCAAAATCCTCGGCGACAAAGAGGAGAGATAGAGAGAGAGAAAGAGGCGAAAATAATGGCGTCGATCCCTCCGCCGGAGCTTGACAACGACTCACTGGCGTCATCGCCGAGATCGGAGTACGATAGTCAGCCACGCGTACGGTTCATGTGTACTTTTGGAGGAAGGATCTTGCCACGTCCGCCGGATAATCAGCTCTGTTACGTCGGCGGCGATAATCGCATGGTCGCTGTTCATCGTCACACTACTTTCGCCTCTCTCCTTAGTAAACTTGCTAAACTCTCCGGTAAAAGCAACATAAGCGTGAAGTACCAGCTACCAAACGAAGATCTTGACGCGTTGATTTCCGTATCAACGGACGAAGATGTAGAGAACATGATGGACGAATACGACCGCGTCGCACAGAATCAAAACCCACGCGCCTCTCGTCTTCGTCTTTTTCTCTTCACCAAAAACGTCGCCGGAGAAGAAGATAACGATAGTCGAGCTAGCAGTATCAGCTCTCTCCTCGATAGCTCTGTTAATCGGGAGCAATGGTTCCTCGACGCTCTTAACCTCGGTTCTTCCGCCGCCGCAACCGCGGTATCTAACGGTGGGTCCGGTAGAGTCTTTGAGCGAGTTAGATCTGAAGTCTCGTCGATTGTTTCTGAAGTTCCTGATTATCTCTTTGGATTGGATAATTTCGATGAAACTGCTCCGCCGCATGAGCTTCGTGATCGTGATCCGAGAGCTAAGATCCAACGAGAAGTCTCGACGCTTTCGGATCCTGGTTCGCCTCGTCGCGATGTTCCTTCGCCGTATGGTTCGACGTCTTCGGCTCCTGTGATGCGAATATCTACACCGGAACTTCCACCACCAGTTTTTATTAAACCGGAAAGTCCAGAACCGGTTTCGACTCCGAAATCCAATCCTCAACCGGAACAAGTAATGCAACAGAGTAATCTCCCGGTTAACTCGCAATGGCAATATGCCCCTGGCCCAGGCCAACAGGTTCATTACCAGGGTCATACCATTCACCAATCACCGGTTTATTACGTTCCTGGTTCGGTTCCGGGTAATCATATGGTCCAGCAAGGAAATCATATGGTTCAACCGGGTAATCATATGGTACAACCGGTTCAAATGCCGGGTCAGTATCTTCAACAATACCATCATGTACCAATGGGATACCACCAACCTCAGACTCATCAGATGGCTGGTCCGGGTCAAGTATATGGTGGAACGGTTAGGCCGGTTATGATGGCAGTTGATGGAATGAACCGGACGGGTTATTATGGAATGAAAACACCCGGTCCGGTTCAAATGTATCAGCATCACACTGGTATGGTTGTTCCTGGTGTAGAAGAACAACAACAATACAGAACCGAAACGGATTCGGATACGGGTCGGGCTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

477

Amino Acids

53.06

Weight (kDa)

6.32

Isoelectric Point (pI)

56.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 75 - 162 1.6e-16 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 848
AccII CGCG 6 cut(s) 174, 374, 432, 456, 617, 819
AclWI GGATC 6 cut(s) 97, 212, 752, 766, 794, 807
AcuI CTGAAG 2 cut(s) 684, 705
AcyI GRCGYC 3 cut(s) 98, 137, 845
AfaI GTAC 6 cut(s) 158, 177, 190, 347, 1146, 1194
AfiI CCNNNNNNNGG 3 cut(s) 112, 1016, 1060
AflIII ACRYGT 2 cut(s) 172, 372
AgeI ACCGGT 3 cut(s) 922, 1060, 1150
AgsI TTSAA 4 cut(s) 1127, 1157, 1178, 1337
AjiI CACGTC 1 cut(s) 215
AjnI CCWGG 6 cut(s) 802, 1009, 1015, 1035, 1075, 1369
AloI GAACNNNNNNTCC 2 cut(s) 557, 589
AluBI AGCT 8 cut(s) 118, 232, 352, 524, 537, 552, 745, 767
AluI AGCT 8 cut(s) 118, 232, 352, 524, 537, 552, 745, 767
Alw26I GTCTC 2 cut(s) 673, 790
AlwI GGATC 6 cut(s) 97, 212, 752, 766, 794, 807
AoxI GGCC 3 cut(s) 1012, 1018, 1265
ArsI GACNNNNNNTTYG 2 cut(s) 1150, 1182
AsiGI ACCGGT 3 cut(s) 922, 1060, 1150
AspLEI GCGC 1 cut(s) 458
AspS9I GGNCC 5 cut(s) 633, 1013, 1102, 1235, 1328
AsuC2I CCSGG 6 cut(s) 983, 1089, 1131, 1164, 1239, 1326
AsuHPI GGTGA 3 cut(s) 475, 1043, 1050
AsuNHI GCTAGC 1 cut(s) 524
AvaII GGWCC 4 cut(s) 633, 1102, 1235, 1328
BaeI ACNNNNGTAYC 2 cut(s) 603, 636
BamHI GGATCC 1 cut(s) 799
BbsI GAAGAC 2 cut(s) 458, 840
BccI CCATC 5 cut(s) 409, 1194, 1222, 1270, 1280
BceAI ACGGC 1 cut(s) 817
BcgI CGANNNNNNTGC 4 cut(s) 710, 728, 744, 762
BciT130I CCWGG 6 cut(s) 804, 1011, 1017, 1037, 1077, 1371
BciVI GTATCC 2 cut(s) 1196, 1408
BcnI CCSGG 6 cut(s) 983, 1089, 1131, 1164, 1239, 1326
BcoDI GTCTC 2 cut(s) 673, 790
BfaI CTAG 1 cut(s) 525
BfuI GTATCC 2 cut(s) 1196, 1408
BglII AGATCT 2 cut(s) 364, 659
BisI GCNGC 4 cut(s) 247, 606, 609, 737
BlsI GCNGC 4 cut(s) 248, 607, 610, 738
Bme18I GGWCC 4 cut(s) 633, 1102, 1235, 1328
BmgBI CACGTC 1 cut(s) 215
BmgT120I GGNCC 5 cut(s) 633, 1013, 1102, 1235, 1328
BmiI GGNNCC 5 cut(s) 575, 634, 801, 855, 1086
BmsI GCATC 2 cut(s) 852, 1357
BmtI GCTAGC 1 cut(s) 528
BpiI GAAGAC 2 cut(s) 458, 840
BpuMI CCSGG 6 cut(s) 983, 1089, 1131, 1164, 1239, 1326
BsaBI GATNNNNATC 1 cut(s) 756
BsaHI GRCGYC 3 cut(s) 98, 137, 845
BsaJI CCNNGG 6 cut(s) 47, 592, 615, 1009, 1015, 1036
BsaXI ACNNNNNCTCC 2 cut(s) 557, 587
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 1016, 1060
Bse118I RCCGGY 4 cut(s) 922, 1060, 1150, 1267
Bse1I ACTGG 3 cut(s) 138, 893, 1360
Bse3DI GCAATG 2 cut(s) 575, 1001
Bse8I GATNNNNATC 1 cut(s) 756
BseBI CCWGG 6 cut(s) 804, 1011, 1017, 1037, 1077, 1371
BseDI CCNNGG 6 cut(s) 47, 592, 615, 1009, 1015, 1036
BseJI GATNNNNATC 1 cut(s) 756
BseLI CCNNNNNNNGG 3 cut(s) 112, 1016, 1060
BseMI GCAATG 2 cut(s) 575, 1001
BseMII CTCAG 1 cut(s) 1229
BseNI ACTGG 3 cut(s) 138, 893, 1360
BseRI GAGGAG 2 cut(s) 75, 533
Bsh1236I CGCG 6 cut(s) 174, 374, 432, 456, 617, 819
Bsh1285I CGRYCG 1 cut(s) 430
BshFI GGCC 3 cut(s) 1014, 1020, 1267
BshTI ACCGGT 3 cut(s) 922, 1060, 1150
BsiEI CGRYCG 1 cut(s) 430
BsiWI CGTACG 1 cut(s) 175
BslI CCNNNNNNNGG 3 cut(s) 112, 1016, 1060
BsmAI GTCTC 2 cut(s) 673, 790
BsnI GGCC 3 cut(s) 1014, 1020, 1267
Bsp143I GATC 9 cut(s) 102, 149, 204, 364, 659, 751, 757, 771, 799
Bsp68I TCGCGA 1 cut(s) 819
BspANI GGCC 3 cut(s) 1014, 1020, 1267
BspCNI CTCAG 1 cut(s) 1228
BspFNI CGCG 6 cut(s) 174, 374, 432, 456, 617, 819
BspLI GGNNCC 5 cut(s) 575, 634, 801, 855, 1086
BspOI GCTAGC 1 cut(s) 528
BspPI GGATC 6 cut(s) 97, 212, 752, 766, 794, 807
BsrDI GCAATG 2 cut(s) 575, 1001
BsrFI RCCGGY 4 cut(s) 922, 1060, 1150, 1267
BsrI ACTGG 3 cut(s) 138, 893, 1360
BssAI RCCGGY 4 cut(s) 922, 1060, 1150, 1267
BssECI CCNNGG 6 cut(s) 47, 592, 615, 1009, 1015, 1036
BssMI GATC 9 cut(s) 102, 149, 204, 364, 659, 751, 757, 771, 799
BssNI GRCGYC 3 cut(s) 98, 137, 845
Bst2UI CCWGG 6 cut(s) 804, 1011, 1017, 1037, 1077, 1371
Bst4CI ACNGT 3 cut(s) 180, 629, 1261
Bst6I CTCTTC 1 cut(s) 485
BstACI GRCGYC 3 cut(s) 98, 137, 845
BstC8I GCNNGC 1 cut(s) 526
BstDEI CTNAG 4 cut(s) 299, 768, 1215, 1431
BstDSI CCRYGG 1 cut(s) 615
BstENI CCTNNNNNAGG 1 cut(s) 1014
BstFNI CGCG 6 cut(s) 174, 374, 432, 456, 617, 819
BstHHI GCGC 1 cut(s) 458
BstKTI GATC 9 cut(s) 105, 152, 207, 367, 662, 754, 760, 774, 802
BstMAI GTCTC 2 cut(s) 673, 790
BstMBI GATC 9 cut(s) 102, 149, 204, 364, 659, 751, 757, 771, 799
BstMCI CGRYCG 1 cut(s) 430
BstMWI GCNNNNNNNGC 6 cut(s) 217, 255, 336, 534, 614, 742
BstNI CCWGG 6 cut(s) 804, 1011, 1017, 1037, 1077, 1371
BstUI CGCG 6 cut(s) 174, 374, 432, 456, 617, 819
BstV2I GAAGAC 2 cut(s) 458, 840
BstX2I RGATCY 5 cut(s) 204, 364, 659, 771, 799
BstYI RGATCY 5 cut(s) 204, 364, 659, 771, 799
BsuI GTATCC 2 cut(s) 1196, 1408
BsuRI GGCC 3 cut(s) 1014, 1020, 1267
BtgI CCRYGG 1 cut(s) 615
BtgZI GCGATG 2 cut(s) 126, 834
BtrI CACGTC 1 cut(s) 215
BtsIMutI CAGTG 2 cut(s) 131, 1353
BtuMI TCGCGA 1 cut(s) 819
Cac8I GCNNGC 1 cut(s) 526
CfoI GCGC 1 cut(s) 458
Cfr10I RCCGGY 4 cut(s) 922, 1060, 1150, 1267
Cfr13I GGNCC 5 cut(s) 633, 1013, 1102, 1235, 1328
Cfr42I CCGCGG 1 cut(s) 618
CpoI CGGWCCG 1 cut(s) 1328
CseI GACGC 6 cut(s) 87, 126, 380, 421, 590, 799
Csp6I GTAC 6 cut(s) 157, 176, 189, 346, 1145, 1193
CspAI ACCGGT 3 cut(s) 922, 1060, 1150
CspI CGGWCCG 1 cut(s) 1328
CviAII CATG 5 cut(s) 184, 259, 412, 740, 1190
CviQI GTAC 6 cut(s) 157, 176, 189, 346, 1145, 1193
DdeI CTNAG 4 cut(s) 299, 768, 1215, 1431
DpnI GATC 9 cut(s) 104, 151, 206, 366, 661, 753, 759, 773, 801
DpnII GATC 9 cut(s) 102, 149, 204, 364, 659, 751, 757, 771, 799
Eam1104I CTCTTC 1 cut(s) 485
EarI CTCTTC 1 cut(s) 485
EciI GGCGGA 4 cut(s) 99, 207, 592, 723
Eco47I GGWCC 4 cut(s) 633, 1102, 1235, 1328
Eco57I CTGAAG 2 cut(s) 684, 705
EcoNI CCTNNNNNAGG 1 cut(s) 1014
EcoRII CCWGG 6 cut(s) 802, 1009, 1015, 1035, 1075, 1369
FaeI CATG 5 cut(s) 187, 262, 415, 743, 1193
FatI CATG 5 cut(s) 183, 258, 411, 739, 1189
FauNDI CATATG 3 cut(s) 1098, 1119, 1140
Fnu4HI GCNGC 4 cut(s) 247, 606, 609, 737
Fsp4HI GCNGC 4 cut(s) 247, 606, 609, 737
FspBI CTAG 1 cut(s) 525
GlaI GCGC 1 cut(s) 457
GluI GCNGC 4 cut(s) 247, 606, 609, 737
HaeIII GGCC 3 cut(s) 1014, 1020, 1267
HgaI GACGC 6 cut(s) 87, 126, 380, 421, 590, 799
HhaI GCGC 1 cut(s) 458
Hin1I GRCGYC 3 cut(s) 98, 137, 845
Hin1II CATG 5 cut(s) 187, 262, 415, 743, 1193
Hin6I GCGC 1 cut(s) 456
HinP1I GCGC 1 cut(s) 456
HincII GTYRAC 1 cut(s) 988
HindII GTYRAC 1 cut(s) 988
HinfI GANTC 7 cut(s) 10, 128, 442, 642, 931, 1219, 1409
HpaI GTTAAC 1 cut(s) 988
HphI GGTGA 3 cut(s) 475, 1043, 1050
Hpy166II GTNNAC 3 cut(s) 189, 305, 988
Hpy188I TCNGA 9 cut(s) 154, 664, 685, 762, 799, 936, 1218, 1227, 1414
Hpy188III TCNNGA 8 cut(s) 368, 563, 692, 749, 755, 787, 818, 917
Hpy8I GTNNAC 3 cut(s) 189, 305, 988
Hpy99I CGWCG 9 cut(s) 103, 245, 437, 497, 584, 676, 793, 819, 847
HpyAV CCTTC 2 cut(s) 195, 837
HpyCH4III ACNGT 3 cut(s) 180, 629, 1261
HpyCH4IV ACGT 5 cut(s) 214, 240, 492, 845, 1071
HpyCH4V TGCA 1 cut(s) 967
HpyF10VI GCNNNNNNNGC 6 cut(s) 217, 255, 336, 534, 614, 742
HpyF3I CTNAG 4 cut(s) 299, 768, 1215, 1431
HpySE526I ACGT 5 cut(s) 214, 240, 492, 845, 1071
Hsp92I GRCGYC 3 cut(s) 98, 137, 845
Hsp92II CATG 5 cut(s) 187, 262, 415, 743, 1193
HspAI GCGC 1 cut(s) 456
KspAI GTTAAC 1 cut(s) 988
KspI CCGCGG 1 cut(s) 618
Kzo9I GATC 9 cut(s) 102, 149, 204, 364, 659, 751, 757, 771, 799
LmnI GCTCC 4 cut(s) 115, 565, 736, 859
LweI GCATC 2 cut(s) 852, 1357
MaeI CTAG 1 cut(s) 525
MaeII ACGT 5 cut(s) 214, 240, 492, 845, 1071
MaeIII GTNAC 2 cut(s) 236, 275
MalI GATC 9 cut(s) 104, 151, 206, 366, 661, 753, 759, 773, 801
MboI GATC 9 cut(s) 102, 149, 204, 364, 659, 751, 757, 771, 799
MflI RGATCY 5 cut(s) 204, 364, 659, 771, 799
MluCI AATT 2 cut(s) 34, 715
MluI ACGCGT 2 cut(s) 172, 372
MlyI GAGTC 4 cut(s) 122, 651, 925, 1213
MmeI TCCRAC 1 cut(s) 799
MseI TTAA 4 cut(s) 558, 588, 903, 987
MslI CAYNNNNRTG 2 cut(s) 338, 1358
MspA1I CMGCKG 1 cut(s) 617
MvaI CCWGG 6 cut(s) 804, 1011, 1017, 1037, 1077, 1371
MvnI CGCG 6 cut(s) 174, 374, 432, 456, 617, 819
MwoI GCNNNNNNNGC 6 cut(s) 217, 255, 336, 534, 614, 742
NciI CCSGG 6 cut(s) 983, 1089, 1131, 1164, 1239, 1326
NdeI CATATG 3 cut(s) 1098, 1119, 1140
NdeII GATC 9 cut(s) 102, 149, 204, 364, 659, 751, 757, 771, 799
NheI GCTAGC 1 cut(s) 524
NlaIII CATG 5 cut(s) 187, 262, 415, 743, 1193
NlaIV GGNNCC 5 cut(s) 575, 634, 801, 855, 1086
NmeAIII GCCGAG 2 cut(s) 28, 171
NmuCI GTSAC 1 cut(s) 275
NruI TCGCGA 1 cut(s) 819
PcsI WCGNNNNNNNCGW 2 cut(s) 246, 460
PfeI GAWTC 3 cut(s) 10, 442, 1409
Pfl23II CGTACG 1 cut(s) 175
PflFI GACNNNGTC 1 cut(s) 431
PinAI ACCGGT 3 cut(s) 922, 1060, 1150
PkrI GCNGC 4 cut(s) 248, 607, 610, 738
PleI GAGTC 4 cut(s) 122, 650, 925, 1213
PpsI GAGTC 4 cut(s) 122, 650, 925, 1213
Psp6I CCWGG 6 cut(s) 802, 1009, 1015, 1035, 1075, 1369
PspGI CCWGG 6 cut(s) 802, 1009, 1015, 1035, 1075, 1369
PspLI CGTACG 1 cut(s) 175
PspN4I GGNNCC 5 cut(s) 575, 634, 801, 855, 1086
PspPI GGNCC 5 cut(s) 633, 1013, 1102, 1235, 1328
PsrI GAACNNNNNNTAC 2 cut(s) 1137, 1169
PsuI RGATCY 5 cut(s) 204, 364, 659, 771, 799
PsyI GACNNNGTC 1 cut(s) 431
RruI TCGCGA 1 cut(s) 819
RsaI GTAC 6 cut(s) 158, 177, 190, 347, 1146, 1194
RsaNI GTAC 6 cut(s) 157, 176, 189, 346, 1145, 1193
RseI CAYNNNNRTG 2 cut(s) 338, 1358
Rsr2I CGGWCCG 1 cut(s) 1328
RsrII CGGWCCG 1 cut(s) 1328
SacII CCGCGG 1 cut(s) 618
SaqAI TTAA 4 cut(s) 558, 588, 903, 987
SatI GCNGC 4 cut(s) 247, 606, 609, 737
Sau3AI GATC 9 cut(s) 102, 149, 204, 364, 659, 751, 757, 771, 799
Sau96I GGNCC 5 cut(s) 633, 1013, 1102, 1235, 1328
SchI GAGTC 4 cut(s) 122, 651, 925, 1213
SfaNI GCATC 2 cut(s) 852, 1357
Sfr303I CCGCGG 1 cut(s) 618
SgrBI CCGCGG 1 cut(s) 618
SinI GGWCC 4 cut(s) 633, 1102, 1235, 1328
SmiMI CAYNNNNRTG 2 cut(s) 338, 1358
Sse9I AATT 2 cut(s) 34, 715
SspMI CTAG 1 cut(s) 525
TaaI ACNGT 3 cut(s) 180, 629, 1261
TaiI ACGT 5 cut(s) 217, 243, 495, 848, 1074
TasI AATT 2 cut(s) 34, 715
TatI WGTACW 1 cut(s) 188
TauI GCSGC 4 cut(s) 249, 608, 611, 739
TfiI GAWTC 3 cut(s) 10, 442, 1409
Tru1I TTAA 4 cut(s) 558, 588, 903, 987
Tru9I TTAA 4 cut(s) 558, 588, 903, 987
TscAI CASTG 2 cut(s) 138, 1360
TseFI GTSAC 1 cut(s) 275
Tsp45I GTSAC 1 cut(s) 275
TspDTI ATGAA 7 cut(s) 17, 172, 260, 738, 1019, 1307, 1331
TspGWI ACGGA 3 cut(s) 373, 407, 1421
TspRI CASTG 2 cut(s) 138, 1360
Tth111I GACNNNGTC 1 cut(s) 431
VpaK11BI GGWCC 4 cut(s) 633, 1102, 1235, 1328
XagI CCTNNNNNAGG 1 cut(s) 1014
XspI CTAG 1 cut(s) 525
ZraI GACGTC 1 cut(s) 846
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.