RLG00000031326

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0007930
Physical Location & Seq
Reverse (-)
4115617 .. 4119118
3502 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031326

Sequence Viewer

Length: 1299 bp
ATGACGTCACTCCACCCCTCCGAGATGGACACCGACTCCGTCACCTCCACTCCGCGATCCGACCACCCCTCAAACGATCAGACACGTGTACGGTTCATGTGCAGCTTCGGTGGGAAGATCCTCCCGCGGCCCCACGATAACCAGCTCCGTTACGTCGGCGGCGACACCAGAATTGTCGCCGTGCAACGCACCACCACTTACGGCTCCCTCCTCTCCAAGCTCTCCAAACTCTCAGGACTGAGTAACGTGACTGTTAAGTACCAGCTTCCGAACGAGGAACTGGATGCTCTGATCTCCGTCACGACGGACGAGGACGTCGACAACATGATGGACGAGTACGACCGCATCACACAGAATCAAAACCCGAAAGCGGCTCGGCTCCGCCTCTTTCTCTTCCCGAAAGGCGACGACGACTCGGCTTCCCGAACCTCCAGCATCAGCTCCCTCCTCGACGGCTCGGCTAAGCGCGAGCACTGGTTCCTCGACGCTCTCAACAGCGGCGCGTCGAACGCTTCGCTCCTCGAACGCGGCCGCTCTGAAGCCTCCTCGATTCTCTCCGAAGTGCCGGACTATCTGTTCGGGTTAGACAACTCCGACGACACTCAAAACAGGGAGTTCAGAGCGAAGACCCGACCCGGAGTTTTACAGGACAACGTATCGGCTTCGGATCCCGGTTCTCCCGCTCCGGTTATCTCGTCGCCGTTTTGCTCCACGTCGTCTAATCCCTCCGTGCCGTCGCTGCCGAACCTCCCGCCGGTGAAGACCAAGCCGGATTCGAAGCCGGAGACGAGAGAGAATCAGTTGGAGGGTTTTGCAGAGGCGACGGCGGAGTTGCCCGTTTCGCAAGCAACAGGGTTTTCGGGTAATCCGGGTAATCCCGGGTTGCATTATATCCAGGACCCGAATTATCCTGGTCATGTTGTGCGCTCAGTACCGGTTTATTATTATCCGGCTCCGGTTCAACAGGCAAACGTACCGGTCCAGCAGGTTCAAATACGGACTCCGTATATGCCTCAGCAATACCAGGCTCTACCGGGTCAGATACCGGTCGGGTATCATAATCAGATGCCGGGTATGGGCCAGGTTTATGGTGGAGGGTTAAGGCCCGTTGCGGGAATGGACCAGTATGACGTGTCGGGTAGGGTTGTTTCTGATGGAGTGAACCAGCAGGTGTACTATGGGGTGAGGAATGGTGGTGTGGTTCCGGGATATAATCCGGCAAGGGTGGTGCAATCGGGGGAAGAATGGCAAGGAAATGGATCCGATATCAATGCGGGTCGCGCCCCCAATGGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

433

Amino Acids

46.82

Weight (kDa)

5.25

Isoelectric Point (pI)

43.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 45 - 133 5.8e-19 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1159
AasI GACNNNNNNGTC 1 cut(s) 314
AatII GACGTC 2 cut(s) 8, 318
Acc36I ACCTGC 2 cut(s) 976, 1159
AccBSI CCGCTC 2 cut(s) 534, 683
AccI GTMKAC 1 cut(s) 318
AccII CGCG 6 cut(s) 55, 127, 468, 503, 528, 1281
AclWI GGATC 6 cut(s) 51, 112, 662, 675, 1254, 1267
AcoI YGGCCR 1 cut(s) 529
AcuI CTGAAG 1 cut(s) 558
AcvI CACGTG 1 cut(s) 86
AcyI GRCGYC 2 cut(s) 5, 315
AfaI GTAC 6 cut(s) 90, 260, 338, 933, 975, 1175
AfiI CCNNNNNNNGG 4 cut(s) 370, 754, 1076, 1112
AflIII ACRYGT 3 cut(s) 83, 85, 1131
AgeI ACCGGT 3 cut(s) 934, 976, 1045
AgsI TTSAA 2 cut(s) 962, 992
AjiI CACGTC 2 cut(s) 714, 1132
AjnI CCWGG 4 cut(s) 894, 910, 1023, 1080
AloI GAACNNNNNNTCC 2 cut(s) 560, 592
AluBI AGCT 5 cut(s) 105, 145, 220, 265, 441
AluI AGCT 5 cut(s) 105, 145, 220, 265, 441
Alw21I GWGCWC 1 cut(s) 474
Alw26I GTCTC 1 cut(s) 779
AlwI GGATC 6 cut(s) 51, 112, 662, 675, 1254, 1267
Ama87I CYCGRG 1 cut(s) 878
AoxI GGCC 4 cut(s) 128, 529, 1078, 1103
ApeKI GCWGC 2 cut(s) 102, 739
ArsI GACNNNNNNTTYG 2 cut(s) 560, 592
AsiGI ACCGGT 3 cut(s) 934, 976, 1045
AspLEI GCGC 4 cut(s) 468, 503, 927, 1283
AspS9I GGNCC 6 cut(s) 129, 898, 979, 1078, 1104, 1120
AsuC2I CCSGG 8 cut(s) 636, 672, 870, 879, 880, 1035, 1071, 1206
AsuHPI GGTGA 3 cut(s) 34, 769, 1195
AsuII TTCGAA 1 cut(s) 776
AvaI CYCGRG 1 cut(s) 878
AvaII GGWCC 3 cut(s) 898, 979, 1120
BamHI GGATCC 2 cut(s) 667, 1259
BbrPI CACGTG 1 cut(s) 86
BbsI GAAGAC 2 cut(s) 632, 767
Bbv12I GWGCWC 1 cut(s) 474
BbvCI CCTCAGC 1 cut(s) 1014
BbvI GCAGC 2 cut(s) 114, 726
BccI CCATC 3 cut(s) 19, 322, 1148
BceAI ACGGC 6 cut(s) 164, 217, 469, 685, 718, 840
BcgI CGANNNNNNTGC 2 cut(s) 1253, 1287
BciT130I CCWGG 4 cut(s) 896, 912, 1025, 1082
BcnI CCSGG 8 cut(s) 636, 672, 870, 879, 880, 1035, 1071, 1206
BcoDI GTCTC 1 cut(s) 779
BfuAI ACCTGC 2 cut(s) 976, 1159
BisI GCNGC 8 cut(s) 103, 128, 160, 372, 499, 529, 532, 740
BlpI GCTNAGC 1 cut(s) 462
BlsI GCNGC 8 cut(s) 104, 129, 161, 373, 500, 530, 533, 741
Bme18I GGWCC 3 cut(s) 898, 979, 1120
BmeT110I CYCGRG 1 cut(s) 878
BmgBI CACGTC 2 cut(s) 714, 1132
BmgT120I GGNCC 6 cut(s) 129, 898, 979, 1078, 1104, 1120
BmiI GGNNCC 9 cut(s) 131, 205, 380, 479, 669, 900, 954, 1203, 1261
BmsI GCATC 4 cut(s) 274, 354, 444, 1056
BpiI GAAGAC 2 cut(s) 632, 767
BpmI CTGGAG 1 cut(s) 415
Bpu10I CCTNAGC 1 cut(s) 1014
Bpu1102I GCTNAGC 1 cut(s) 462
Bpu14I TTCGAA 1 cut(s) 776
BpuMI CCSGG 8 cut(s) 636, 672, 870, 879, 880, 1035, 1071, 1206
BsaAI YACGTR 1 cut(s) 86
BsaHI GRCGYC 2 cut(s) 5, 315
BsaJI CCNNGG 2 cut(s) 125, 878
BsaWI WCCGGW 5 cut(s) 685, 934, 955, 976, 1045
BsaXI ACNNNNNCTCC 4 cut(s) 20, 34, 50, 64
Bsc4I CCNNNNNNNGG 4 cut(s) 370, 754, 1076, 1112
Bse118I RCCGGY 4 cut(s) 754, 934, 976, 1045
Bse1I ACTGG 3 cut(s) 285, 479, 1123
BseBI CCWGG 4 cut(s) 896, 912, 1025, 1082
BseDI CCNNGG 2 cut(s) 125, 878
BseGI GGATG 1 cut(s) 289
BseLI CCNNNNNNNGG 4 cut(s) 370, 754, 1076, 1112
BseMII CTCAG 4 cut(s) 230, 246, 942, 1028
BseNI ACTGG 3 cut(s) 285, 479, 1123
BseRI GAGGAG 4 cut(s) 200, 437, 509, 535
BseX3I CGGCCG 1 cut(s) 529
BseXI GCAGC 2 cut(s) 114, 726
BsgI GTGCAG 1 cut(s) 121
Bsh1236I CGCG 6 cut(s) 55, 127, 468, 503, 528, 1281
Bsh1285I CGRYCG 3 cut(s) 343, 532, 1050
BshFI GGCC 4 cut(s) 130, 531, 1080, 1105
BshTI ACCGGT 3 cut(s) 934, 976, 1045
BsiEI CGRYCG 3 cut(s) 343, 532, 1050
BsiHKAI GWGCWC 1 cut(s) 474
BsiHKCI CYCGRG 1 cut(s) 878
BslI CCNNNNNNNGG 4 cut(s) 370, 754, 1076, 1112
BsmAI GTCTC 1 cut(s) 779
BsmBI CGTCTC 1 cut(s) 779
BsnI GGCC 4 cut(s) 130, 531, 1080, 1105
BsoBI CYCGRG 1 cut(s) 878
Bsp119I TTCGAA 1 cut(s) 776
Bsp1286I GDGCHC 1 cut(s) 474
Bsp143I GATC 7 cut(s) 56, 76, 117, 291, 667, 1259, 1292
Bsp1720I GCTNAGC 1 cut(s) 462
BspANI GGCC 4 cut(s) 130, 531, 1080, 1105
BspCNI CTCAG 4 cut(s) 231, 245, 941, 1027
BspFNI CGCG 6 cut(s) 55, 127, 468, 503, 528, 1281
BspLI GGNNCC 9 cut(s) 131, 205, 380, 479, 669, 900, 954, 1203, 1261
BspMI ACCTGC 2 cut(s) 976, 1159
BspPI GGATC 6 cut(s) 51, 112, 662, 675, 1254, 1267
BspT104I TTCGAA 1 cut(s) 776
BsrBI CCGCTC 2 cut(s) 534, 683
BsrFI RCCGGY 4 cut(s) 754, 934, 976, 1045
BsrI ACTGG 3 cut(s) 285, 479, 1123
BssAI RCCGGY 4 cut(s) 754, 934, 976, 1045
BssECI CCNNGG 2 cut(s) 125, 878
BssMI GATC 7 cut(s) 56, 76, 117, 291, 667, 1259, 1292
BssNI GRCGYC 2 cut(s) 5, 315
Bst2UI CCWGG 4 cut(s) 896, 912, 1025, 1082
Bst4CI ACNGT 2 cut(s) 93, 253
Bst6I CTCTTC 1 cut(s) 398
BstACI GRCGYC 2 cut(s) 5, 315
BstBAI YACGTR 1 cut(s) 86
BstBI TTCGAA 1 cut(s) 776
BstC8I GCNNGC 2 cut(s) 470, 846
BstDEI CTNAG 5 cut(s) 232, 239, 462, 928, 1014
BstDSI CCRYGG 1 cut(s) 125
BstF5I GGATG 1 cut(s) 289
BstFNI CGCG 6 cut(s) 55, 127, 468, 503, 528, 1281
BstHHI GCGC 4 cut(s) 468, 503, 927, 1283
BstKTI GATC 7 cut(s) 59, 79, 120, 294, 670, 1262, 1295
BstMAI GTCTC 1 cut(s) 779
BstMBI GATC 7 cut(s) 56, 76, 117, 291, 667, 1259, 1292
BstMCI CGRYCG 3 cut(s) 343, 532, 1050
BstMWI GCNNNNNNNGC 4 cut(s) 509, 739, 841, 1280
BstNI CCWGG 4 cut(s) 896, 912, 1025, 1082
BstUI CGCG 6 cut(s) 55, 127, 468, 503, 528, 1281
BstV1I GCAGC 2 cut(s) 114, 726
BstV2I GAAGAC 2 cut(s) 632, 767
BstX2I RGATCY 4 cut(s) 117, 667, 1259, 1292
BstXI CCANNNNNNTGG 1 cut(s) 1088
BstYI RGATCY 4 cut(s) 117, 667, 1259, 1292
BstZI CGGCCG 1 cut(s) 529
BsuRI GGCC 4 cut(s) 130, 531, 1080, 1105
BtgI CCRYGG 1 cut(s) 125
BtrI CACGTC 2 cut(s) 714, 1132
BtsCI GGATG 1 cut(s) 289
BtsIMutI CAGTG 1 cut(s) 472
BveI ACCTGC 2 cut(s) 976, 1159
Cac8I GCNNGC 2 cut(s) 470, 846
CciNI GCGGCCGC 1 cut(s) 529
CfoI GCGC 4 cut(s) 468, 503, 927, 1283
Cfr10I RCCGGY 4 cut(s) 754, 934, 976, 1045
Cfr13I GGNCC 6 cut(s) 129, 898, 979, 1078, 1104, 1120
Cfr42I CCGCGG 1 cut(s) 128
Cfr9I CCCGGG 1 cut(s) 878
CseI GACGC 2 cut(s) 492, 494
Csp6I GTAC 6 cut(s) 89, 259, 337, 932, 974, 1174
CspAI ACCGGT 3 cut(s) 934, 976, 1045
CviAII CATG 3 cut(s) 97, 325, 917
CviQI GTAC 6 cut(s) 89, 259, 337, 932, 974, 1174
DdeI CTNAG 5 cut(s) 232, 239, 462, 928, 1014
DpnI GATC 7 cut(s) 58, 78, 119, 293, 669, 1261, 1294
DpnII GATC 7 cut(s) 56, 76, 117, 291, 667, 1259, 1292
DrdI GACNNNNNNGTC 1 cut(s) 314
DseDI GACNNNNNNGTC 1 cut(s) 314
EaeI YGGCCR 1 cut(s) 529
EagI CGGCCG 1 cut(s) 529
Eam1104I CTCTTC 1 cut(s) 398
EarI CTCTTC 1 cut(s) 398
EciI GGCGGA 2 cut(s) 371, 842
EclXI CGGCCG 1 cut(s) 529
Eco32I GATATC 1 cut(s) 1267
Eco47I GGWCC 3 cut(s) 898, 979, 1120
Eco52I CGGCCG 1 cut(s) 529
Eco57I CTGAAG 1 cut(s) 558
Eco72I CACGTG 1 cut(s) 86
Eco88I CYCGRG 1 cut(s) 878
EcoO109I RGGNCCY 1 cut(s) 898
EcoRII CCWGG 4 cut(s) 894, 910, 1023, 1080
EcoRV GATATC 1 cut(s) 1267
Esp3I CGTCTC 1 cut(s) 779
FaeI CATG 3 cut(s) 100, 328, 920
FatI CATG 3 cut(s) 96, 324, 916
FauI CCCGC 5 cut(s) 132, 688, 759, 1105, 1267
FblI GTMKAC 1 cut(s) 318
Fnu4HI GCNGC 8 cut(s) 103, 128, 160, 372, 499, 529, 532, 740
FokI GGATG 1 cut(s) 296
Fsp4HI GCNGC 8 cut(s) 103, 128, 160, 372, 499, 529, 532, 740
GlaI GCGC 4 cut(s) 467, 502, 926, 1282
GluI GCNGC 8 cut(s) 103, 128, 160, 372, 499, 529, 532, 740
GsuI CTGGAG 1 cut(s) 415
HaeIII GGCC 4 cut(s) 130, 531, 1080, 1105
HgaI GACGC 2 cut(s) 492, 494
HhaI GCGC 4 cut(s) 468, 503, 927, 1283
Hin1I GRCGYC 2 cut(s) 5, 315
Hin1II CATG 3 cut(s) 100, 328, 920
Hin6I GCGC 4 cut(s) 466, 501, 925, 1281
HinP1I GCGC 4 cut(s) 466, 501, 925, 1281
HincII GTYRAC 1 cut(s) 319
HindII GTYRAC 1 cut(s) 319
HinfI GANTC 7 cut(s) 35, 355, 413, 550, 773, 796, 1000
HphI GGTGA 3 cut(s) 34, 769, 1195
Hpy166II GTNNAC 4 cut(s) 89, 319, 1162, 1174
Hpy188III TCNNGA 5 cut(s) 234, 301, 397, 423, 1296
Hpy8I GTNNAC 4 cut(s) 89, 319, 1162, 1174
HpyCH4III ACNGT 2 cut(s) 93, 253
HpyCH4IV ACGT 9 cut(s) 5, 85, 153, 246, 315, 654, 713, 972, 1131
HpyCH4V TGCA 5 cut(s) 102, 184, 815, 886, 1231
HpyF10VI GCNNNNNNNGC 4 cut(s) 509, 739, 841, 1280
HpyF3I CTNAG 5 cut(s) 232, 239, 462, 928, 1014
HpySE526I ACGT 9 cut(s) 5, 85, 153, 246, 315, 654, 713, 972, 1131
Hsp92I GRCGYC 2 cut(s) 5, 315
Hsp92II CATG 3 cut(s) 100, 328, 920
HspAI GCGC 4 cut(s) 466, 501, 925, 1281
KspI CCGCGG 1 cut(s) 128
Kzo9I GATC 7 cut(s) 56, 76, 117, 291, 667, 1259, 1292
LmnI GCTCC 8 cut(s) 150, 209, 384, 446, 522, 688, 713, 958
Lsp1109I GCAGC 2 cut(s) 114, 726
LweI GCATC 4 cut(s) 274, 354, 444, 1056
MaeII ACGT 9 cut(s) 5, 85, 153, 246, 315, 654, 713, 972, 1131
MaeIII GTNAC 6 cut(s) 6, 40, 149, 242, 247, 298
MalI GATC 7 cut(s) 58, 78, 119, 293, 669, 1261, 1294
MbiI CCGCTC 2 cut(s) 534, 683
MboI GATC 7 cut(s) 56, 76, 117, 291, 667, 1259, 1292
MboII GAAGA 5 cut(s) 127, 385, 637, 772, 1253
MflI RGATCY 4 cut(s) 117, 667, 1259, 1292
MhlI GDGCHC 1 cut(s) 474
MluCI AATT 2 cut(s) 171, 904
MlyI GAGTC 3 cut(s) 29, 407, 994
MmeI TCCRAC 3 cut(s) 84, 618, 783
MseI TTAA 2 cut(s) 255, 1100
MspA1I CMGCKG 2 cut(s) 127, 498
MvaI CCWGG 4 cut(s) 896, 912, 1025, 1082
MvnI CGCG 6 cut(s) 55, 127, 468, 503, 528, 1281
MwoI GCNNNNNNNGC 4 cut(s) 509, 739, 841, 1280
NciI CCSGG 8 cut(s) 636, 672, 870, 879, 880, 1035, 1071, 1206
NdeII GATC 7 cut(s) 56, 76, 117, 291, 667, 1259, 1292
NlaIII CATG 3 cut(s) 100, 328, 920
NlaIV GGNNCC 9 cut(s) 131, 205, 380, 479, 669, 900, 954, 1203, 1261
NmeAIII GCCGAG 3 cut(s) 355, 395, 437
NmuCI GTSAC 4 cut(s) 6, 40, 247, 298
NotI GCGGCCGC 1 cut(s) 529
NspV TTCGAA 1 cut(s) 776
PaqCI CACCTGC 1 cut(s) 1159
PcsI WCGNNNNNNNCGW 2 cut(s) 159, 315
PfeI GAWTC 4 cut(s) 355, 550, 773, 796
PflFI GACNNNGTC 1 cut(s) 38
PfoI TCCNGGA 2 cut(s) 894, 1204
PinAI ACCGGT 3 cut(s) 934, 976, 1045
PkrI GCNGC 8 cut(s) 104, 129, 161, 373, 500, 530, 533, 741
PleI GAGTC 3 cut(s) 29, 407, 994
PmaCI CACGTG 1 cut(s) 86
PmlI CACGTG 1 cut(s) 86
PpsI GAGTC 3 cut(s) 29, 407, 994
Ppu21I YACGTR 1 cut(s) 86
PpuMI RGGWCCY 1 cut(s) 898
Psp5II RGGWCCY 1 cut(s) 898
Psp6I CCWGG 4 cut(s) 894, 910, 1023, 1080
PspCI CACGTG 1 cut(s) 86
PspGI CCWGG 4 cut(s) 894, 910, 1023, 1080
PspN4I GGNNCC 9 cut(s) 131, 205, 380, 479, 669, 900, 954, 1203, 1261
PspPI GGNCC 6 cut(s) 129, 898, 979, 1078, 1104, 1120
PspPPI RGGWCCY 1 cut(s) 898
PsuI RGATCY 4 cut(s) 117, 667, 1259, 1292
PsyI GACNNNGTC 1 cut(s) 38
RsaI GTAC 6 cut(s) 90, 260, 338, 933, 975, 1175
RsaNI GTAC 6 cut(s) 89, 259, 337, 932, 974, 1174
SacII CCGCGG 1 cut(s) 128
SalI GTCGAC 1 cut(s) 317
SaqAI TTAA 2 cut(s) 255, 1100
SatI GCNGC 8 cut(s) 103, 128, 160, 372, 499, 529, 532, 740
Sau3AI GATC 7 cut(s) 56, 76, 117, 291, 667, 1259, 1292
Sau96I GGNCC 6 cut(s) 129, 898, 979, 1078, 1104, 1120
SchI GAGTC 3 cut(s) 29, 407, 994
SduI GDGCHC 1 cut(s) 474
SfaNI GCATC 4 cut(s) 274, 354, 444, 1056
Sfr303I CCGCGG 1 cut(s) 128
SfuI TTCGAA 1 cut(s) 776
SgrAI CRCCGGYG 1 cut(s) 754
SgrBI CCGCGG 1 cut(s) 128
SinI GGWCC 3 cut(s) 898, 979, 1120
SmaI CCCGGG 1 cut(s) 880
Sse9I AATT 2 cut(s) 171, 904
TaaI ACNGT 2 cut(s) 93, 253
TaiI ACGT 9 cut(s) 8, 88, 156, 249, 318, 657, 716, 975, 1134
TaqI TCGA 7 cut(s) 318, 450, 483, 506, 522, 548, 776
TasI AATT 2 cut(s) 171, 904
TatI WGTACW 1 cut(s) 1173
TauI GCSGC 6 cut(s) 130, 162, 374, 501, 531, 534
TfiI GAWTC 4 cut(s) 355, 550, 773, 796
Tru1I TTAA 2 cut(s) 255, 1100
Tru9I TTAA 2 cut(s) 255, 1100
TscAI CASTG 1 cut(s) 479
TseFI GTSAC 4 cut(s) 6, 40, 247, 298
TseI GCWGC 2 cut(s) 102, 739
Tsp45I GTSAC 4 cut(s) 6, 40, 247, 298
TspDTI ATGAA 1 cut(s) 85
TspGWI ACGGA 7 cut(s) 28, 137, 286, 320, 718, 993, 1012
TspMI CCCGGG 1 cut(s) 878
TspRI CASTG 1 cut(s) 479
Tth111I GACNNNGTC 1 cut(s) 38
VpaK11BI GGWCC 3 cut(s) 898, 979, 1120
XcmI CCANNNNNNNNNTGG 1 cut(s) 1088
XmaI CCCGGG 1 cut(s) 878
XmiI GTMKAC 1 cut(s) 318
ZraI GACGTC 2 cut(s) 6, 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.