Rh5BG044300

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0007930
Physical Location & Seq
Reverse (-)
3512059 .. 3515010
2952 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG044300.1

Sequence Viewer

Length: 1290 bp
ATGACGTCACTCCACCCCTCCGAGATGGACACCGACTCCGTCACCTCCACTCCGCGATCCGACCACCCCTCAAACGATCAGACACGTGTACGGTTCATGTGCAGCTTCGGCGGGAAGATCCTCCCGCGGCCCCACGATAACCAGCTCCGTTACGTCGGCGGCGACACCAGAATTGTCGCCGTGCAACGCGCCACCACTTACGGCTCCCTCCTCTCCAAGCTCTCCAAACTCTCAGGACTGAGTAACGTGACCGTCAAGTACCAGCTTCCGAACGAGGAACTGGATGCTCTGATCTCCGTCACGACGGACGAGGACGTCGACAACATGATGGACGAGTACGACCGCATCACACAGAATCAAAACCCGAAAGCGGCTCGGCTCCGCCTCTTTCTCTTCCCCAAAGGCGACGACGACTCGGCTTCCCGAACCTCCAGCATCAGCTCGCTCCTCGACGGCTCGGCTAAGCGCGAGCACTGGTTCCTCGACGCGCTCAACAGCGGCGCGTCGAACGCTTCGCTCCTCGAGCGCGGCCGTTCTGAAGCCTCCTCGATTCTCTCCGAAGTGCCGGACTATCTGTTCGGGTTGGACAACTCCGACGACACTCACAACCGGGAGTTCAGAGCGAAGACCCGACCCGGGGTTTTACAGGACAACGTATCGGCTTCGGATCCCGGTTCTCCCGCTCCGGTTATCTCGTCGCCGTTTTGCTCCACGTCGTCTAATCCCTCCGTGCCGTCGCTTCCGAATCTCCCGCCGGTGAAGACCAAGCCGGATTCGAAGCCGGAGACGAGAGAGAATCAGTCGGAGGGTTTTGCAGAGACGACGGCGGAGTTGCCCGTTTCGCAAGCAACAGGGTTTTCGGGTAATGCCGGGTTGCATTATATCCAGGACCCGAATTATCCGGGTCATGTTGTGCGCTCAGTACCGGTTTATTATTACCCGGCTCCGGTTCAACCGGCAAACGTACCGGTCCAGCAGGTTCAAATACGGACTCCGTATATGCCTCAGCAATACCAGGCTCTACCGGGTCAGATACCGGTCGGGTATCATAATCAGATGCCGGGTATGGGCCAGGTTTATGGTGGAGGGTTAAGGCCCGTTGCGGGAATGGACCAGTATGACGTCACGGGTAGGGTTGTTTCTGATGGAGTGAACCAGCAGGTGTACTATGGGGTTAGGAATGGTGGTGTGGTTCCGGGATATAATCCGGCAAGGGTGGTGCAATCGGGGGAAGAATGGCAAGGAAATGGATCCGATATCAATGCGGGTCGCGCCCCGAATGGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

429

Amino Acids

46.49

Weight (kDa)

5.33

Isoelectric Point (pI)

46.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 45 - 133 1e-18 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1150
AasI GACNNNNNNGTC 1 cut(s) 314
AatII GACGTC 3 cut(s) 8, 318, 1125
Acc36I ACCTGC 2 cut(s) 967, 1150
AccBSI CCGCTC 1 cut(s) 683
AccI GTMKAC 1 cut(s) 318
AccII CGCG 8 cut(s) 55, 127, 189, 468, 488, 503, 528, 1272
AclWI GGATC 6 cut(s) 51, 112, 662, 675, 1245, 1258
AcoI YGGCCR 1 cut(s) 529
AcuI CTGAAG 1 cut(s) 558
AcvI CACGTG 1 cut(s) 86
AcyI GRCGYC 3 cut(s) 5, 315, 1122
AfaI GTAC 6 cut(s) 90, 260, 338, 924, 966, 1166
AfiI CCNNNNNNNGG 6 cut(s) 370, 636, 637, 946, 1067, 1103
AflIII ACRYGT 2 cut(s) 83, 85
AgeI ACCGGT 3 cut(s) 925, 967, 1036
AgsI TTSAA 2 cut(s) 953, 983
AjiI CACGTC 1 cut(s) 714
AjnI CCWGG 3 cut(s) 885, 1014, 1071
AloI GAACNNNNNNTCC 2 cut(s) 560, 592
AluBI AGCT 5 cut(s) 105, 145, 220, 265, 441
AluI AGCT 5 cut(s) 105, 145, 220, 265, 441
Alw21I GWGCWC 1 cut(s) 474
Alw26I GTCTC 2 cut(s) 779, 812
AlwI GGATC 6 cut(s) 51, 112, 662, 675, 1245, 1258
Ama87I CYCGRG 2 cut(s) 521, 635
AoxI GGCC 4 cut(s) 128, 529, 1069, 1094
ApeKI GCWGC 1 cut(s) 102
ArsI GACNNNNNNTTYG 2 cut(s) 560, 592
AsiGI ACCGGT 3 cut(s) 925, 967, 1036
AspLEI GCGC 7 cut(s) 191, 468, 490, 503, 528, 918, 1274
AspS9I GGNCC 6 cut(s) 129, 889, 970, 1069, 1095, 1111
AsuHPI GGTGA 2 cut(s) 34, 769
AsuII TTCGAA 1 cut(s) 776
AvaI CYCGRG 2 cut(s) 521, 635
AvaII GGWCC 3 cut(s) 889, 970, 1111
BamHI GGATCC 2 cut(s) 667, 1250
BbrPI CACGTG 1 cut(s) 86
BbsI GAAGAC 2 cut(s) 632, 767
Bbv12I GWGCWC 1 cut(s) 474
BbvCI CCTCAGC 1 cut(s) 1005
BbvI GCAGC 1 cut(s) 114
BccI CCATC 3 cut(s) 19, 322, 1139
BceAI ACGGC 7 cut(s) 164, 217, 469, 516, 685, 718, 840
BcgI CGANNNNNNTGC 2 cut(s) 1244, 1278
BciT130I CCWGG 3 cut(s) 887, 1016, 1073
BcoDI GTCTC 2 cut(s) 779, 812
BfuAI ACCTGC 2 cut(s) 967, 1150
BisI GCNGC 6 cut(s) 103, 128, 160, 372, 499, 529
BlpI GCTNAGC 1 cut(s) 462
BlsI GCNGC 6 cut(s) 104, 129, 161, 373, 500, 530
Bme18I GGWCC 3 cut(s) 889, 970, 1111
BmeT110I CYCGRG 2 cut(s) 521, 635
BmgBI CACGTC 1 cut(s) 714
BmgT120I GGNCC 6 cut(s) 129, 889, 970, 1069, 1095, 1111
BmiI GGNNCC 9 cut(s) 131, 205, 380, 479, 669, 891, 945, 1194, 1252
BmsI GCATC 4 cut(s) 274, 354, 444, 1047
BpiI GAAGAC 2 cut(s) 632, 767
BpmI CTGGAG 1 cut(s) 415
Bpu10I CCTNAGC 1 cut(s) 1005
Bpu1102I GCTNAGC 1 cut(s) 462
Bpu14I TTCGAA 1 cut(s) 776
BsaAI YACGTR 1 cut(s) 86
BsaHI GRCGYC 3 cut(s) 5, 315, 1122
BsaJI CCNNGG 3 cut(s) 125, 635, 636
BsaWI WCCGGW 5 cut(s) 685, 925, 946, 967, 1036
BsaXI ACNNNNNCTCC 4 cut(s) 20, 34, 50, 64
Bsc4I CCNNNNNNNGG 6 cut(s) 370, 636, 637, 946, 1067, 1103
Bse118I RCCGGY 5 cut(s) 754, 925, 955, 967, 1036
Bse1I ACTGG 3 cut(s) 285, 479, 1114
BseBI CCWGG 3 cut(s) 887, 1016, 1073
BseDI CCNNGG 3 cut(s) 125, 635, 636
BseGI GGATG 1 cut(s) 289
BseLI CCNNNNNNNGG 6 cut(s) 370, 636, 637, 946, 1067, 1103
BseMII CTCAG 4 cut(s) 230, 246, 933, 1019
BseNI ACTGG 3 cut(s) 285, 479, 1114
BseRI GAGGAG 4 cut(s) 200, 437, 509, 535
BseX3I CGGCCG 1 cut(s) 529
BseXI GCAGC 1 cut(s) 114
BsgI GTGCAG 1 cut(s) 121
Bsh1236I CGCG 8 cut(s) 55, 127, 189, 468, 488, 503, 528, 1272
Bsh1285I CGRYCG 3 cut(s) 343, 532, 1041
BshFI GGCC 4 cut(s) 130, 531, 1071, 1096
BshTI ACCGGT 3 cut(s) 925, 967, 1036
BsiEI CGRYCG 3 cut(s) 343, 532, 1041
BsiHKAI GWGCWC 1 cut(s) 474
BsiHKCI CYCGRG 2 cut(s) 521, 635
BslI CCNNNNNNNGG 6 cut(s) 370, 636, 637, 946, 1067, 1103
BsmAI GTCTC 2 cut(s) 779, 812
BsmBI CGTCTC 2 cut(s) 779, 812
BsnI GGCC 4 cut(s) 130, 531, 1071, 1096
BsoBI CYCGRG 2 cut(s) 521, 635
Bsp119I TTCGAA 1 cut(s) 776
Bsp1286I GDGCHC 1 cut(s) 474
Bsp143I GATC 7 cut(s) 56, 76, 117, 291, 667, 1250, 1283
Bsp1720I GCTNAGC 1 cut(s) 462
BspANI GGCC 4 cut(s) 130, 531, 1071, 1096
BspCNI CTCAG 4 cut(s) 231, 245, 932, 1018
BspFNI CGCG 8 cut(s) 55, 127, 189, 468, 488, 503, 528, 1272
BspLI GGNNCC 9 cut(s) 131, 205, 380, 479, 669, 891, 945, 1194, 1252
BspMI ACCTGC 2 cut(s) 967, 1150
BspPI GGATC 6 cut(s) 51, 112, 662, 675, 1245, 1258
BspT104I TTCGAA 1 cut(s) 776
BsrBI CCGCTC 1 cut(s) 683
BsrFI RCCGGY 5 cut(s) 754, 925, 955, 967, 1036
BsrI ACTGG 3 cut(s) 285, 479, 1114
BssAI RCCGGY 5 cut(s) 754, 925, 955, 967, 1036
BssECI CCNNGG 3 cut(s) 125, 635, 636
BssMI GATC 7 cut(s) 56, 76, 117, 291, 667, 1250, 1283
BssNI GRCGYC 3 cut(s) 5, 315, 1122
Bst2UI CCWGG 3 cut(s) 887, 1016, 1073
Bst4CI ACNGT 2 cut(s) 93, 253
Bst6I CTCTTC 1 cut(s) 398
BstACI GRCGYC 3 cut(s) 5, 315, 1122
BstBAI YACGTR 1 cut(s) 86
BstBI TTCGAA 1 cut(s) 776
BstC8I GCNNGC 3 cut(s) 443, 470, 846
BstDEI CTNAG 5 cut(s) 232, 239, 462, 919, 1005
BstDSI CCRYGG 1 cut(s) 125
BstF5I GGATG 1 cut(s) 289
BstFNI CGCG 8 cut(s) 55, 127, 189, 468, 488, 503, 528, 1272
BstHHI GCGC 7 cut(s) 191, 468, 490, 503, 528, 918, 1274
BstKTI GATC 7 cut(s) 59, 79, 120, 294, 670, 1253, 1286
BstMAI GTCTC 2 cut(s) 779, 812
BstMBI GATC 7 cut(s) 56, 76, 117, 291, 667, 1250, 1283
BstMCI CGRYCG 3 cut(s) 343, 532, 1041
BstMWI GCNNNNNNNGC 5 cut(s) 108, 509, 523, 841, 1271
BstNI CCWGG 3 cut(s) 887, 1016, 1073
BstUI CGCG 8 cut(s) 55, 127, 189, 468, 488, 503, 528, 1272
BstV1I GCAGC 1 cut(s) 114
BstV2I GAAGAC 2 cut(s) 632, 767
BstX2I RGATCY 4 cut(s) 117, 667, 1250, 1283
BstXI CCANNNNNNTGG 1 cut(s) 1079
BstYI RGATCY 4 cut(s) 117, 667, 1250, 1283
BstZI CGGCCG 1 cut(s) 529
BsuRI GGCC 4 cut(s) 130, 531, 1071, 1096
BtgI CCRYGG 1 cut(s) 125
BtrI CACGTC 1 cut(s) 714
BtsCI GGATG 1 cut(s) 289
BtsIMutI CAGTG 1 cut(s) 472
BveI ACCTGC 2 cut(s) 967, 1150
Cac8I GCNNGC 3 cut(s) 443, 470, 846
CfoI GCGC 7 cut(s) 191, 468, 490, 503, 528, 918, 1274
Cfr10I RCCGGY 5 cut(s) 754, 925, 955, 967, 1036
Cfr13I GGNCC 6 cut(s) 129, 889, 970, 1069, 1095, 1111
Cfr42I CCGCGG 1 cut(s) 128
Cfr9I CCCGGG 1 cut(s) 635
CseI GACGC 2 cut(s) 492, 494
Csp6I GTAC 6 cut(s) 89, 259, 337, 923, 965, 1165
CspAI ACCGGT 3 cut(s) 925, 967, 1036
CviAII CATG 3 cut(s) 97, 325, 908
CviQI GTAC 6 cut(s) 89, 259, 337, 923, 965, 1165
DdeI CTNAG 5 cut(s) 232, 239, 462, 919, 1005
DpnI GATC 7 cut(s) 58, 78, 119, 293, 669, 1252, 1285
DpnII GATC 7 cut(s) 56, 76, 117, 291, 667, 1250, 1283
DrdI GACNNNNNNGTC 1 cut(s) 314
DseDI GACNNNNNNGTC 1 cut(s) 314
EaeI YGGCCR 1 cut(s) 529
EagI CGGCCG 1 cut(s) 529
Eam1104I CTCTTC 1 cut(s) 398
EarI CTCTTC 1 cut(s) 398
EciI GGCGGA 2 cut(s) 371, 842
EclXI CGGCCG 1 cut(s) 529
Eco32I GATATC 1 cut(s) 1258
Eco47I GGWCC 3 cut(s) 889, 970, 1111
Eco52I CGGCCG 1 cut(s) 529
Eco57I CTGAAG 1 cut(s) 558
Eco72I CACGTG 1 cut(s) 86
Eco88I CYCGRG 2 cut(s) 521, 635
EcoO109I RGGNCCY 1 cut(s) 889
EcoRII CCWGG 3 cut(s) 885, 1014, 1071
EcoRV GATATC 1 cut(s) 1258
Esp3I CGTCTC 2 cut(s) 779, 812
FaeI CATG 3 cut(s) 100, 328, 911
FatI CATG 3 cut(s) 96, 324, 907
FauI CCCGC 6 cut(s) 104, 132, 688, 759, 1096, 1258
FblI GTMKAC 1 cut(s) 318
Fnu4HI GCNGC 6 cut(s) 103, 128, 160, 372, 499, 529
FokI GGATG 1 cut(s) 296
Fsp4HI GCNGC 6 cut(s) 103, 128, 160, 372, 499, 529
GlaI GCGC 7 cut(s) 190, 467, 489, 502, 527, 917, 1273
GluI GCNGC 6 cut(s) 103, 128, 160, 372, 499, 529
GsuI CTGGAG 1 cut(s) 415
HaeIII GGCC 4 cut(s) 130, 531, 1071, 1096
HgaI GACGC 2 cut(s) 492, 494
HhaI GCGC 7 cut(s) 191, 468, 490, 503, 528, 918, 1274
Hin1I GRCGYC 3 cut(s) 5, 315, 1122
Hin1II CATG 3 cut(s) 100, 328, 911
Hin6I GCGC 7 cut(s) 189, 466, 488, 501, 526, 916, 1272
HinP1I GCGC 7 cut(s) 189, 466, 488, 501, 526, 916, 1272
HincII GTYRAC 1 cut(s) 319
HindII GTYRAC 1 cut(s) 319
HinfI GANTC 8 cut(s) 35, 355, 413, 550, 745, 773, 796, 991
HphI GGTGA 2 cut(s) 34, 769
Hpy166II GTNNAC 4 cut(s) 89, 319, 1153, 1165
Hpy188III TCNNGA 4 cut(s) 234, 301, 423, 1287
Hpy8I GTNNAC 4 cut(s) 89, 319, 1153, 1165
HpyCH4III ACNGT 2 cut(s) 93, 253
HpyCH4IV ACGT 9 cut(s) 5, 85, 153, 246, 315, 654, 713, 963, 1122
HpyCH4V TGCA 5 cut(s) 102, 184, 815, 877, 1222
HpyF10VI GCNNNNNNNGC 5 cut(s) 108, 509, 523, 841, 1271
HpyF3I CTNAG 5 cut(s) 232, 239, 462, 919, 1005
HpySE526I ACGT 9 cut(s) 5, 85, 153, 246, 315, 654, 713, 963, 1122
Hsp92I GRCGYC 3 cut(s) 5, 315, 1122
Hsp92II CATG 3 cut(s) 100, 328, 911
HspAI GCGC 7 cut(s) 189, 466, 488, 501, 526, 916, 1272
KspI CCGCGG 1 cut(s) 128
Kzo9I GATC 7 cut(s) 56, 76, 117, 291, 667, 1250, 1283
LmnI GCTCC 8 cut(s) 150, 209, 384, 450, 522, 688, 713, 949
Lsp1109I GCAGC 1 cut(s) 114
LweI GCATC 4 cut(s) 274, 354, 444, 1047
MaeII ACGT 9 cut(s) 5, 85, 153, 246, 315, 654, 713, 963, 1122
MaeIII GTNAC 7 cut(s) 6, 40, 149, 242, 247, 298, 1123
MalI GATC 7 cut(s) 58, 78, 119, 293, 669, 1252, 1285
MbiI CCGCTC 1 cut(s) 683
MboI GATC 7 cut(s) 56, 76, 117, 291, 667, 1250, 1283
MboII GAAGA 5 cut(s) 127, 385, 637, 772, 1244
MflI RGATCY 4 cut(s) 117, 667, 1250, 1283
MhlI GDGCHC 1 cut(s) 474
MluCI AATT 2 cut(s) 171, 895
MlyI GAGTC 3 cut(s) 29, 407, 985
MmeI TCCRAC 4 cut(s) 84, 564, 618, 783
MseI TTAA 1 cut(s) 1091
MspA1I CMGCKG 2 cut(s) 127, 498
MvaI CCWGG 3 cut(s) 887, 1016, 1073
MvnI CGCG 8 cut(s) 55, 127, 189, 468, 488, 503, 528, 1272
MwoI GCNNNNNNNGC 5 cut(s) 108, 509, 523, 841, 1271
NdeII GATC 7 cut(s) 56, 76, 117, 291, 667, 1250, 1283
NlaIII CATG 3 cut(s) 100, 328, 911
NlaIV GGNNCC 9 cut(s) 131, 205, 380, 479, 669, 891, 945, 1194, 1252
NmeAIII GCCGAG 3 cut(s) 355, 395, 437
NmuCI GTSAC 5 cut(s) 6, 40, 247, 298, 1123
NspV TTCGAA 1 cut(s) 776
PaeR7I CTCGAG 1 cut(s) 521
PaqCI CACCTGC 1 cut(s) 1150
PcsI WCGNNNNNNNCGW 2 cut(s) 159, 315
PfeI GAWTC 5 cut(s) 355, 550, 745, 773, 796
PflFI GACNNNGTC 1 cut(s) 38
PfoI TCCNGGA 2 cut(s) 885, 1195
PinAI ACCGGT 3 cut(s) 925, 967, 1036
PkrI GCNGC 6 cut(s) 104, 129, 161, 373, 500, 530
PleI GAGTC 3 cut(s) 29, 407, 985
PmaCI CACGTG 1 cut(s) 86
PmlI CACGTG 1 cut(s) 86
PpsI GAGTC 3 cut(s) 29, 407, 985
Ppu21I YACGTR 1 cut(s) 86
PpuMI RGGWCCY 1 cut(s) 889
Psp5II RGGWCCY 1 cut(s) 889
Psp6I CCWGG 3 cut(s) 885, 1014, 1071
PspCI CACGTG 1 cut(s) 86
PspGI CCWGG 3 cut(s) 885, 1014, 1071
PspN4I GGNNCC 9 cut(s) 131, 205, 380, 479, 669, 891, 945, 1194, 1252
PspPI GGNCC 6 cut(s) 129, 889, 970, 1069, 1095, 1111
PspPPI RGGWCCY 1 cut(s) 889
PspXI VCTCGAGB 1 cut(s) 521
PsuI RGATCY 4 cut(s) 117, 667, 1250, 1283
PsyI GACNNNGTC 1 cut(s) 38
RsaI GTAC 6 cut(s) 90, 260, 338, 924, 966, 1166
RsaNI GTAC 6 cut(s) 89, 259, 337, 923, 965, 1165
SacII CCGCGG 1 cut(s) 128
SalI GTCGAC 1 cut(s) 317
SaqAI TTAA 1 cut(s) 1091
SatI GCNGC 6 cut(s) 103, 128, 160, 372, 499, 529
Sau3AI GATC 7 cut(s) 56, 76, 117, 291, 667, 1250, 1283
Sau96I GGNCC 6 cut(s) 129, 889, 970, 1069, 1095, 1111
SchI GAGTC 3 cut(s) 29, 407, 985
SduI GDGCHC 1 cut(s) 474
SfaNI GCATC 4 cut(s) 274, 354, 444, 1047
Sfr274I CTCGAG 1 cut(s) 521
Sfr303I CCGCGG 1 cut(s) 128
SfuI TTCGAA 1 cut(s) 776
SgrAI CRCCGGYG 1 cut(s) 754
SgrBI CCGCGG 1 cut(s) 128
SinI GGWCC 3 cut(s) 889, 970, 1111
SlaI CTCGAG 1 cut(s) 521
SmaI CCCGGG 1 cut(s) 637
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
Sse9I AATT 2 cut(s) 171, 895
TaaI ACNGT 2 cut(s) 93, 253
TaiI ACGT 9 cut(s) 8, 88, 156, 249, 318, 657, 716, 966, 1125
TaqI TCGA 7 cut(s) 318, 450, 483, 506, 522, 548, 776
TasI AATT 2 cut(s) 171, 895
TatI WGTACW 1 cut(s) 1164
TauI GCSGC 5 cut(s) 130, 162, 374, 501, 531
TfiI GAWTC 5 cut(s) 355, 550, 745, 773, 796
Tru1I TTAA 1 cut(s) 1091
Tru9I TTAA 1 cut(s) 1091
TscAI CASTG 1 cut(s) 479
TseFI GTSAC 5 cut(s) 6, 40, 247, 298, 1123
TseI GCWGC 1 cut(s) 102
Tsp45I GTSAC 5 cut(s) 6, 40, 247, 298, 1123
TspDTI ATGAA 1 cut(s) 85
TspGWI ACGGA 7 cut(s) 28, 137, 286, 320, 718, 984, 1003
TspMI CCCGGG 1 cut(s) 635
TspRI CASTG 1 cut(s) 479
Tth111I GACNNNGTC 1 cut(s) 38
VpaK11BI GGWCC 3 cut(s) 889, 970, 1111
XcmI CCANNNNNNNNNTGG 1 cut(s) 1079
XhoI CTCGAG 1 cut(s) 521
XmaI CCCGGG 1 cut(s) 635
XmiI GTMKAC 1 cut(s) 318
ZraI GACGTC 3 cut(s) 6, 316, 1123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.