Rroxscaffold_1G00070080

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0007930
Physical Location & Seq
Forward (+)
90931549 .. 90932922
1374 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00070080.1

Sequence Viewer

Length: 1317 bp
ATGACGTCACTCCACCCCTCAGAGATGGACACCGACTCCGTCACCTCCACTCCGCGATCCGACCACCCCTCAAACGATCAGACACGTGTGCGGTTCATGTGCAGCTTCGGCGGGAAGATCCTCCCGCGGCCCCACGATAACCAGCTCCGTTATGTCGGCGGCGACACCAGAATTGTCGCCGTGCAACGCGCCACCACTTACGGCTCCCTCCTCTCCAAGCTCTCCAAACTCTCAGGCAGGCCAAAAAAAAAAAAACGACGTCGACTGAGTAACGTGACCGTTAAGTACCAGCTTCCGAATGAGGAACTGGATGCTCTGATCTCCGTCACGACGGACGAGGACGTCGACAACATGATGGACGAGTACGACCGCATCACACAGAATCAAAACCCGAAAGCGGCTCGGCTCCGCCTCTTTCTCTTCCCCAAAGGCGACGACGACTCGGCTTCCCGAACCTCCAGCATCAGTTCGCTCCTCGACGGCTCGGCTAAGCGCGAGCACTGGTTCCTCGACGCGCTCAACAGCGGCGCGTCGAACTCTTCGCTCCTCGAGCGCGGCCGTTCTGAAGCCTCCTCGATTCTCTCCGAAGTGCCGGACTATCTGTTCGGGTTGGACAACTCCGACGACACTCACAACCGGGAGTTCAGAGGGAAGACCCGACCCGGGGTTTTACAGGACAACATATCGGCTTCGGATCCCGGTTCTCCCGCTCCGGTTATCTCGTCGCCGTTTTGCTCCACGTCTTCAAATCCTTCCGTGCCGTCTCTGCCGAATCTCCCGCCGGTGAAGACCAAGCCGGATTCGAAGCCGGAGACGAGAGAGAATCAGTCGGAGGGTTTTGCAGAGACGACGGCGGAGTTGCCCGTTTCGCAAGCAACAAGTTTTTCGGGTAATCCCGGGTTGCATTATATCCAGGACCCGAATTATCCGGGTCATGTTGTGCGCTCAGTTCCTGTTTATTATTACCCGGCTCCGGTTCAACCGGCAAACGTACCAGTCCAGCAGGTTCAAATACGGACTCCGTATATGCCTCAGCAATACCAGGCTCTACCGGGTCAGATACCGGTCGGGTATCATAATCAGATGCCGGGTATGGGCCAGGTTTATGGTGGAGGTTTAAGGCCCGTTGCGGGAATGGACCAGTATGACGTGTCGGGTAGGGTTGTTTCTGATGGAGTGAACCAGCAGGTGTACTATGGGGTGAGGAATGGTGGTGTGGTTCCGGGATATAATCCGGCAAGGGTGGTGCAATCGGGGGAAGAATGGCAAGGAAATGTATCCGATATCAATGCGGGTCGCGCCCCGAATGGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

438

Amino Acids

47.82

Weight (kDa)

6.47

Isoelectric Point (pI)

51.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 45 - 142 2.8e-16 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1177
AasI GACNNNNNNGTC 1 cut(s) 341
AatII GACGTC 3 cut(s) 8, 262, 345
Acc36I ACCTGC 2 cut(s) 994, 1177
AccBSI CCGCTC 1 cut(s) 710
AccI GTMKAC 2 cut(s) 262, 345
AccII CGCG 8 cut(s) 55, 127, 189, 495, 515, 530, 555, 1299
AclWI GGATC 4 cut(s) 51, 112, 689, 702
AcoI YGGCCR 1 cut(s) 556
AcuI CTGAAG 1 cut(s) 585
AcvI CACGTG 1 cut(s) 86
AcyI GRCGYC 3 cut(s) 5, 259, 342
AfaI GTAC 4 cut(s) 287, 365, 993, 1193
AfiI CCNNNNNNNGG 6 cut(s) 397, 663, 664, 973, 1094, 1130
AflIII ACRYGT 3 cut(s) 83, 85, 1149
AgeI ACCGGT 1 cut(s) 1063
AgsI TTSAA 3 cut(s) 747, 980, 1010
AjiI CACGTC 2 cut(s) 741, 1150
AjnI CCWGG 3 cut(s) 912, 1041, 1098
AloI GAACNNNNNNTCC 2 cut(s) 587, 619
AluBI AGCT 4 cut(s) 105, 145, 220, 292
AluI AGCT 4 cut(s) 105, 145, 220, 292
Alw21I GWGCWC 1 cut(s) 501
Alw26I GTCTC 3 cut(s) 768, 806, 839
AlwI GGATC 4 cut(s) 51, 112, 689, 702
AlwNI CAGNNNCTG 1 cut(s) 953
Ama87I CYCGRG 3 cut(s) 548, 662, 896
AoxI GGCC 5 cut(s) 128, 239, 556, 1096, 1121
ApeKI GCWGC 1 cut(s) 102
ArsI GACNNNNNNTTYG 2 cut(s) 587, 619
AsiGI ACCGGT 1 cut(s) 1063
AspLEI GCGC 7 cut(s) 191, 495, 517, 530, 555, 945, 1301
AspS9I GGNCC 5 cut(s) 129, 916, 1096, 1122, 1138
AsuHPI GGTGA 3 cut(s) 34, 796, 1213
AsuII TTCGAA 1 cut(s) 803
AvaI CYCGRG 3 cut(s) 548, 662, 896
AvaII GGWCC 2 cut(s) 916, 1138
BamHI GGATCC 1 cut(s) 694
BbrPI CACGTG 1 cut(s) 86
BbsI GAAGAC 3 cut(s) 659, 735, 794
Bbv12I GWGCWC 1 cut(s) 501
BbvCI CCTCAGC 1 cut(s) 1032
BbvI GCAGC 1 cut(s) 114
BccI CCATC 3 cut(s) 19, 349, 1166
BceAI ACGGC 7 cut(s) 164, 217, 496, 543, 712, 745, 867
BcgI CGANNNNNNTGC 2 cut(s) 1271, 1305
BciT130I CCWGG 3 cut(s) 914, 1043, 1100
BciVI GTATCC 1 cut(s) 1288
BcoDI GTCTC 3 cut(s) 768, 806, 839
BfuAI ACCTGC 2 cut(s) 994, 1177
BfuI GTATCC 1 cut(s) 1288
BisI GCNGC 6 cut(s) 103, 128, 160, 399, 526, 556
BlpI GCTNAGC 1 cut(s) 489
BlsI GCNGC 6 cut(s) 104, 129, 161, 400, 527, 557
Bme18I GGWCC 2 cut(s) 916, 1138
BmeT110I CYCGRG 3 cut(s) 548, 662, 896
BmgBI CACGTC 2 cut(s) 741, 1150
BmgT120I GGNCC 5 cut(s) 129, 916, 1096, 1122, 1138
BmiI GGNNCC 8 cut(s) 131, 205, 407, 506, 696, 918, 972, 1221
BmsI GCATC 4 cut(s) 301, 381, 471, 1074
BpiI GAAGAC 3 cut(s) 659, 735, 794
BpmI CTGGAG 1 cut(s) 442
Bpu10I CCTNAGC 1 cut(s) 1032
Bpu1102I GCTNAGC 1 cut(s) 489
Bpu14I TTCGAA 1 cut(s) 803
BsaAI YACGTR 1 cut(s) 86
BsaHI GRCGYC 3 cut(s) 5, 259, 342
BsaJI CCNNGG 4 cut(s) 125, 662, 663, 896
BsaWI WCCGGW 3 cut(s) 712, 973, 1063
BsaXI ACNNNNNCTCC 4 cut(s) 20, 34, 50, 64
Bsc4I CCNNNNNNNGG 6 cut(s) 397, 663, 664, 973, 1094, 1130
Bse118I RCCGGY 3 cut(s) 781, 982, 1063
Bse1I ACTGG 4 cut(s) 312, 506, 995, 1141
BseBI CCWGG 3 cut(s) 914, 1043, 1100
BseDI CCNNGG 4 cut(s) 125, 662, 663, 896
BseGI GGATG 1 cut(s) 316
BseLI CCNNNNNNNGG 6 cut(s) 397, 663, 664, 973, 1094, 1130
BseMII CTCAG 5 cut(s) 33, 246, 257, 960, 1046
BseNI ACTGG 4 cut(s) 312, 506, 995, 1141
BseRI GAGGAG 4 cut(s) 200, 464, 536, 562
BseX3I CGGCCG 1 cut(s) 556
BseXI GCAGC 1 cut(s) 114
BsgI GTGCAG 1 cut(s) 121
Bsh1236I CGCG 8 cut(s) 55, 127, 189, 495, 515, 530, 555, 1299
Bsh1285I CGRYCG 3 cut(s) 370, 559, 1068
BshFI GGCC 5 cut(s) 130, 241, 558, 1098, 1123
BshTI ACCGGT 1 cut(s) 1063
BsiEI CGRYCG 3 cut(s) 370, 559, 1068
BsiHKAI GWGCWC 1 cut(s) 501
BsiHKCI CYCGRG 3 cut(s) 548, 662, 896
BslI CCNNNNNNNGG 6 cut(s) 397, 663, 664, 973, 1094, 1130
BsmAI GTCTC 3 cut(s) 768, 806, 839
BsmBI CGTCTC 3 cut(s) 768, 806, 839
BsnI GGCC 5 cut(s) 130, 241, 558, 1098, 1123
BsoBI CYCGRG 3 cut(s) 548, 662, 896
Bsp119I TTCGAA 1 cut(s) 803
Bsp1286I GDGCHC 1 cut(s) 501
Bsp143I GATC 6 cut(s) 56, 76, 117, 318, 694, 1310
Bsp1720I GCTNAGC 1 cut(s) 489
BspANI GGCC 5 cut(s) 130, 241, 558, 1098, 1123
BspCNI CTCAG 5 cut(s) 32, 245, 258, 959, 1045
BspFNI CGCG 8 cut(s) 55, 127, 189, 495, 515, 530, 555, 1299
BspLI GGNNCC 8 cut(s) 131, 205, 407, 506, 696, 918, 972, 1221
BspMI ACCTGC 2 cut(s) 994, 1177
BspPI GGATC 4 cut(s) 51, 112, 689, 702
BspT104I TTCGAA 1 cut(s) 803
BsrBI CCGCTC 1 cut(s) 710
BsrFI RCCGGY 3 cut(s) 781, 982, 1063
BsrI ACTGG 4 cut(s) 312, 506, 995, 1141
BssAI RCCGGY 3 cut(s) 781, 982, 1063
BssECI CCNNGG 4 cut(s) 125, 662, 663, 896
BssMI GATC 6 cut(s) 56, 76, 117, 318, 694, 1310
BssNI GRCGYC 3 cut(s) 5, 259, 342
Bst2UI CCWGG 3 cut(s) 914, 1043, 1100
Bst4CI ACNGT 1 cut(s) 280
Bst6I CTCTTC 2 cut(s) 425, 544
BstACI GRCGYC 3 cut(s) 5, 259, 342
BstBAI YACGTR 1 cut(s) 86
BstBI TTCGAA 1 cut(s) 803
BstC8I GCNNGC 3 cut(s) 239, 497, 873
BstDEI CTNAG 6 cut(s) 19, 232, 266, 489, 946, 1032
BstDSI CCRYGG 1 cut(s) 125
BstF5I GGATG 1 cut(s) 316
BstFNI CGCG 8 cut(s) 55, 127, 189, 495, 515, 530, 555, 1299
BstHHI GCGC 7 cut(s) 191, 495, 517, 530, 555, 945, 1301
BstKTI GATC 6 cut(s) 59, 79, 120, 321, 697, 1313
BstMAI GTCTC 3 cut(s) 768, 806, 839
BstMBI GATC 6 cut(s) 56, 76, 117, 318, 694, 1310
BstMCI CGRYCG 3 cut(s) 370, 559, 1068
BstMWI GCNNNNNNNGC 5 cut(s) 108, 550, 766, 868, 1298
BstNI CCWGG 3 cut(s) 914, 1043, 1100
BstUI CGCG 8 cut(s) 55, 127, 189, 495, 515, 530, 555, 1299
BstV1I GCAGC 1 cut(s) 114
BstV2I GAAGAC 3 cut(s) 659, 735, 794
BstX2I RGATCY 3 cut(s) 117, 694, 1310
BstXI CCANNNNNNTGG 1 cut(s) 1106
BstYI RGATCY 3 cut(s) 117, 694, 1310
BstZI CGGCCG 1 cut(s) 556
BsuI GTATCC 1 cut(s) 1288
BsuRI GGCC 5 cut(s) 130, 241, 558, 1098, 1123
BtgI CCRYGG 1 cut(s) 125
BtrI CACGTC 2 cut(s) 741, 1150
BtsCI GGATG 1 cut(s) 316
BtsIMutI CAGTG 1 cut(s) 499
BveI ACCTGC 2 cut(s) 994, 1177
Cac8I GCNNGC 3 cut(s) 239, 497, 873
CaiI CAGNNNCTG 1 cut(s) 953
CfoI GCGC 7 cut(s) 191, 495, 517, 530, 555, 945, 1301
Cfr10I RCCGGY 3 cut(s) 781, 982, 1063
Cfr13I GGNCC 5 cut(s) 129, 916, 1096, 1122, 1138
Cfr42I CCGCGG 1 cut(s) 128
Cfr9I CCCGGG 2 cut(s) 662, 896
CseI GACGC 2 cut(s) 519, 521
Csp6I GTAC 4 cut(s) 286, 364, 992, 1192
CspAI ACCGGT 1 cut(s) 1063
CviAII CATG 3 cut(s) 97, 352, 935
CviQI GTAC 4 cut(s) 286, 364, 992, 1192
DdeI CTNAG 6 cut(s) 19, 232, 266, 489, 946, 1032
DpnI GATC 6 cut(s) 58, 78, 119, 320, 696, 1312
DpnII GATC 6 cut(s) 56, 76, 117, 318, 694, 1310
DrdI GACNNNNNNGTC 1 cut(s) 341
DseDI GACNNNNNNGTC 1 cut(s) 341
EaeI YGGCCR 1 cut(s) 556
EagI CGGCCG 1 cut(s) 556
Eam1104I CTCTTC 2 cut(s) 425, 544
EarI CTCTTC 2 cut(s) 425, 544
EciI GGCGGA 2 cut(s) 398, 869
EclXI CGGCCG 1 cut(s) 556
Eco32I GATATC 1 cut(s) 1285
Eco47I GGWCC 2 cut(s) 916, 1138
Eco52I CGGCCG 1 cut(s) 556
Eco57I CTGAAG 1 cut(s) 585
Eco72I CACGTG 1 cut(s) 86
Eco88I CYCGRG 3 cut(s) 548, 662, 896
EcoO109I RGGNCCY 1 cut(s) 916
EcoRII CCWGG 3 cut(s) 912, 1041, 1098
EcoRV GATATC 1 cut(s) 1285
Esp3I CGTCTC 3 cut(s) 768, 806, 839
FaeI CATG 3 cut(s) 100, 355, 938
FatI CATG 3 cut(s) 96, 351, 934
FauI CCCGC 6 cut(s) 104, 132, 715, 786, 1123, 1285
FblI GTMKAC 2 cut(s) 262, 345
Fnu4HI GCNGC 6 cut(s) 103, 128, 160, 399, 526, 556
FokI GGATG 1 cut(s) 323
Fsp4HI GCNGC 6 cut(s) 103, 128, 160, 399, 526, 556
GlaI GCGC 7 cut(s) 190, 494, 516, 529, 554, 944, 1300
GluI GCNGC 6 cut(s) 103, 128, 160, 399, 526, 556
GsuI CTGGAG 1 cut(s) 442
HaeIII GGCC 5 cut(s) 130, 241, 558, 1098, 1123
HgaI GACGC 2 cut(s) 519, 521
HhaI GCGC 7 cut(s) 191, 495, 517, 530, 555, 945, 1301
Hin1I GRCGYC 3 cut(s) 5, 259, 342
Hin1II CATG 3 cut(s) 100, 355, 938
Hin6I GCGC 7 cut(s) 189, 493, 515, 528, 553, 943, 1299
HinP1I GCGC 7 cut(s) 189, 493, 515, 528, 553, 943, 1299
HincII GTYRAC 2 cut(s) 263, 346
HindII GTYRAC 2 cut(s) 263, 346
HinfI GANTC 8 cut(s) 35, 382, 440, 577, 772, 800, 823, 1018
HphI GGTGA 3 cut(s) 34, 796, 1213
Hpy166II GTNNAC 4 cut(s) 263, 346, 1180, 1192
Hpy188III TCNNGA 3 cut(s) 328, 450, 1314
Hpy8I GTNNAC 4 cut(s) 263, 346, 1180, 1192
HpyAV CCTTC 1 cut(s) 762
HpyCH4III ACNGT 1 cut(s) 280
HpyCH4IV ACGT 8 cut(s) 5, 85, 259, 273, 342, 740, 990, 1149
HpyCH4V TGCA 5 cut(s) 102, 184, 842, 904, 1249
HpyF10VI GCNNNNNNNGC 5 cut(s) 108, 550, 766, 868, 1298
HpyF3I CTNAG 6 cut(s) 19, 232, 266, 489, 946, 1032
HpySE526I ACGT 8 cut(s) 5, 85, 259, 273, 342, 740, 990, 1149
Hsp92I GRCGYC 3 cut(s) 5, 259, 342
Hsp92II CATG 3 cut(s) 100, 355, 938
HspAI GCGC 7 cut(s) 189, 493, 515, 528, 553, 943, 1299
KspI CCGCGG 1 cut(s) 128
Kzo9I GATC 6 cut(s) 56, 76, 117, 318, 694, 1310
LmnI GCTCC 8 cut(s) 150, 209, 411, 477, 549, 715, 740, 976
Lsp1109I GCAGC 1 cut(s) 114
LweI GCATC 4 cut(s) 301, 381, 471, 1074
MaeII ACGT 8 cut(s) 5, 85, 259, 273, 342, 740, 990, 1149
MaeIII GTNAC 5 cut(s) 6, 40, 269, 274, 325
MalI GATC 6 cut(s) 58, 78, 119, 320, 696, 1312
MbiI CCGCTC 1 cut(s) 710
MboI GATC 6 cut(s) 56, 76, 117, 318, 694, 1310
MboII GAAGA 7 cut(s) 127, 412, 531, 664, 735, 799, 1271
MflI RGATCY 3 cut(s) 117, 694, 1310
MhlI GDGCHC 1 cut(s) 501
MluCI AATT 2 cut(s) 171, 922
MlyI GAGTC 3 cut(s) 29, 434, 1012
MmeI TCCRAC 4 cut(s) 84, 591, 645, 810
MseI TTAA 2 cut(s) 282, 1118
MspA1I CMGCKG 2 cut(s) 127, 525
MvaI CCWGG 3 cut(s) 914, 1043, 1100
MvnI CGCG 8 cut(s) 55, 127, 189, 495, 515, 530, 555, 1299
MwoI GCNNNNNNNGC 5 cut(s) 108, 550, 766, 868, 1298
NdeII GATC 6 cut(s) 56, 76, 117, 318, 694, 1310
NlaIII CATG 3 cut(s) 100, 355, 938
NlaIV GGNNCC 8 cut(s) 131, 205, 407, 506, 696, 918, 972, 1221
NmeAIII GCCGAG 3 cut(s) 382, 422, 464
NmuCI GTSAC 4 cut(s) 6, 40, 274, 325
NspV TTCGAA 1 cut(s) 803
PaeR7I CTCGAG 1 cut(s) 548
PaqCI CACCTGC 1 cut(s) 1177
PcsI WCGNNNNNNNCGW 1 cut(s) 342
PfeI GAWTC 5 cut(s) 382, 577, 772, 800, 823
PflFI GACNNNGTC 1 cut(s) 38
PfoI TCCNGGA 2 cut(s) 912, 1222
PinAI ACCGGT 1 cut(s) 1063
PkrI GCNGC 6 cut(s) 104, 129, 161, 400, 527, 557
PleI GAGTC 3 cut(s) 29, 434, 1012
PmaCI CACGTG 1 cut(s) 86
PmlI CACGTG 1 cut(s) 86
PpsI GAGTC 3 cut(s) 29, 434, 1012
Ppu21I YACGTR 1 cut(s) 86
PpuMI RGGWCCY 1 cut(s) 916
Psp5II RGGWCCY 1 cut(s) 916
Psp6I CCWGG 3 cut(s) 912, 1041, 1098
PspCI CACGTG 1 cut(s) 86
PspGI CCWGG 3 cut(s) 912, 1041, 1098
PspN4I GGNNCC 8 cut(s) 131, 205, 407, 506, 696, 918, 972, 1221
PspPI GGNCC 5 cut(s) 129, 916, 1096, 1122, 1138
PspPPI RGGWCCY 1 cut(s) 916
PspXI VCTCGAGB 1 cut(s) 548
PstNI CAGNNNCTG 1 cut(s) 953
PsuI RGATCY 3 cut(s) 117, 694, 1310
PsyI GACNNNGTC 1 cut(s) 38
RsaI GTAC 4 cut(s) 287, 365, 993, 1193
RsaNI GTAC 4 cut(s) 286, 364, 992, 1192
SacII CCGCGG 1 cut(s) 128
SalI GTCGAC 2 cut(s) 261, 344
SaqAI TTAA 2 cut(s) 282, 1118
SatI GCNGC 6 cut(s) 103, 128, 160, 399, 526, 556
Sau3AI GATC 6 cut(s) 56, 76, 117, 318, 694, 1310
Sau96I GGNCC 5 cut(s) 129, 916, 1096, 1122, 1138
SchI GAGTC 3 cut(s) 29, 434, 1012
SduI GDGCHC 1 cut(s) 501
SfaNI GCATC 4 cut(s) 301, 381, 471, 1074
Sfr274I CTCGAG 1 cut(s) 548
Sfr303I CCGCGG 1 cut(s) 128
SfuI TTCGAA 1 cut(s) 803
SgrAI CRCCGGYG 1 cut(s) 781
SgrBI CCGCGG 1 cut(s) 128
SinI GGWCC 2 cut(s) 916, 1138
SlaI CTCGAG 1 cut(s) 548
SmaI CCCGGG 2 cut(s) 664, 898
SmlI CTYRAG 1 cut(s) 548
SmoI CTYRAG 1 cut(s) 548
Sse9I AATT 2 cut(s) 171, 922
TaaI ACNGT 1 cut(s) 280
TaiI ACGT 8 cut(s) 8, 88, 262, 276, 345, 743, 993, 1152
TaqI TCGA 8 cut(s) 262, 345, 477, 510, 533, 549, 575, 803
TasI AATT 2 cut(s) 171, 922
TatI WGTACW 1 cut(s) 1191
TauI GCSGC 5 cut(s) 130, 162, 401, 528, 558
TfiI GAWTC 5 cut(s) 382, 577, 772, 800, 823
Tru1I TTAA 2 cut(s) 282, 1118
Tru9I TTAA 2 cut(s) 282, 1118
TscAI CASTG 1 cut(s) 506
TseFI GTSAC 4 cut(s) 6, 40, 274, 325
TseI GCWGC 1 cut(s) 102
Tsp45I GTSAC 4 cut(s) 6, 40, 274, 325
TspDTI ATGAA 1 cut(s) 85
TspGWI ACGGA 7 cut(s) 28, 137, 313, 347, 745, 1011, 1030
TspMI CCCGGG 2 cut(s) 662, 896
TspRI CASTG 1 cut(s) 506
Tth111I GACNNNGTC 1 cut(s) 38
VpaK11BI GGWCC 2 cut(s) 916, 1138
XcmI CCANNNNNNNNNTGG 1 cut(s) 1106
XhoI CTCGAG 1 cut(s) 548
XmaI CCCGGG 2 cut(s) 662, 896
XmiI GTMKAC 2 cut(s) 262, 345
ZraI GACGTC 3 cut(s) 6, 260, 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.