MD05G1327000.v1.1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0007930
Physical Location & Seq
Forward (+)
45310638 .. 45312396
1759 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1327000.v1.1.491

Sequence Viewer

Length: 1344 bp
ATGGCGTCACTCCACCCCTCCGAGTTGGACTCCGCCGCCACCGACTCGGTCGCCTCGACCCCGCGCTCGGACCACCCGTCTCACGATCTCAACTCGCGTGTACGGTTCATGTGCAGCTTCGGCGGCAAGATCTTGCCTCGCCCGCACGACAATCAGCTTCGCTATGTCGGAGGCGATACCCGAATTGTCGCCGTCCAGCGCACCACCTCTTTCTCCGCCCTTCTCTCGAAGTTATCTAAACTCTCAGGGTTGAGTGACGTGACGGTGAAGTACCAGCTGCCGAACGAGGACCTCGACTCGTTGATCTCTGTCACCACCGACGAGGACGTCGACAACATGATGGAGGAGTACGACCGCGTCGCACAGAATCTAAACTCAAAGTCGGCGCGGCTCCGCCTCTTTCTCTTCGTCAAAGGCAGCGACGATTCGATTAACTCGCGATCCTCCAGCATCAGCTCGCTCCTCGACGGCTCCGCCAAGCGCGAGCTCTGGTTCCTCGACGCTCTCAACAGCGGCGCGTCCAATTCCGCGATGCTCGAGCGGAACCGCTCCGAAGCTTCTTCGATCTTCTCCGAAGTGCCTGACTACTTATTCGGGTTGGACAATTCCGACGACACTCCCTCTCGCGGTGACGAGTTCAAACCGAAGGCCCGACCCGTATTGCATGACAACGTGTCGGTTTCGGATCCGGGTTCACCTGCCCCGCTAACTTCCTCGCCGTTCTGCTCGACGTCGTCTGTGCCATCCGTGCCGTCGATGCCGAGTCTACCACCGGTGAAAACCCGACCCGATTCCAATCAAGCCATCGAGAATCAAGCCATCGAGACGAAAGAGAATCAATCGGAGGGATTTTCCGAGACGGTTGAGTTGCCCGTGTCGCAAGCAACTGGGTTCGCGGGCAACCCCGGGGTGCATTATATGCCGGATCCTAATTACCCGGGTCATATGGTTCAGCCAGTACCCGTTTACTATTATCCGGGTCAGGTTCCACCCACAACCGTACAGGTCCAGCCGTTTCCGATTCAGAGTCAGTATGTCCATCAGCAATACCACCAAGTAGGGAGTCAGATTCCAATGGGGTACCATAACCCAATACCAGGTATGGGTCAAATATATGGCGGAGGGTTGAGGCCAGTGGCGGGAATGGACCCGTATGACGTGTCAGCTCGGGTGGTTTCCAATGGAGTGAGCCAGCAGCAGCAGCAGCAACAGGTGTTCTATGGAGTGAGGAATGCTGCGGTGGTTCCGCCGTATAATCCAGGTATGGTGGTGCAATCGGGGGAGGAATGGCAAGGACCCGGACCCGAGATGAATAAGGGTCGAGCTCCGAATGCAACATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

448

Amino Acids

48.5

Weight (kDa)

5.15

Isoelectric Point (pI)

55.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 49 - 137 1.6e-20 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 706
AasI GACNNNNNNGTC 1 cut(s) 326
AatII GACGTC 2 cut(s) 330, 734
Acc36I ACCTGC 1 cut(s) 706
Acc65I GGTACC 1 cut(s) 1080
AccB1I GGYRCC 1 cut(s) 1080
AccBSI CCGCTC 2 cut(s) 541, 549
AccI GTMKAC 2 cut(s) 330, 766
AclWI GGATC 5 cut(s) 435, 680, 693, 920, 933
AcyI GRCGYC 3 cut(s) 5, 327, 731
AfaI GTAC 6 cut(s) 102, 272, 350, 960, 1002, 1082
AfiI CCNNNNNNNGG 5 cut(s) 67, 626, 1097, 1103, 1139
AflIII ACRYGT 2 cut(s) 672, 1158
AgeI ACCGGT 1 cut(s) 772
AgsI TTSAA 1 cut(s) 640
AhdI GACNNNNNGTC 2 cut(s) 76, 673
AjiI CACGTC 2 cut(s) 259, 1159
AjnI CCWGG 2 cut(s) 1096, 1258
AluBI AGCT 8 cut(s) 117, 157, 277, 456, 487, 557, 1166, 1325
AluI AGCT 8 cut(s) 117, 157, 277, 456, 487, 557, 1166, 1325
Alw21I GWGCWC 2 cut(s) 489, 1327
Alw26I GTCTC 3 cut(s) 84, 818, 851
AlwI GGATC 5 cut(s) 435, 680, 693, 920, 933
Ama87I CYCGRG 5 cut(s) 536, 905, 937, 1167, 1304
AoxI GGCC 2 cut(s) 648, 1130
ApeKI GCWGC 8 cut(s) 114, 277, 417, 1195, 1198, 1201, 1204, 1235
ArsI GACNNNNNNTTYG 2 cut(s) 575, 607
AsiGI ACCGGT 1 cut(s) 772
Asp718I GGTACC 1 cut(s) 1080
AspLEI GCGC 5 cut(s) 66, 201, 388, 483, 518
AspS9I GGNCC 7 cut(s) 70, 289, 649, 1006, 1147, 1295, 1301
AsuC2I CCSGG 7 cut(s) 690, 906, 907, 938, 939, 978, 1299
AsuHPI GGTGA 5 cut(s) 277, 304, 641, 687, 787
AvaI CYCGRG 5 cut(s) 536, 905, 937, 1167, 1304
AvaII GGWCC 6 cut(s) 70, 289, 1006, 1147, 1295, 1301
BamHI GGATCC 2 cut(s) 685, 925
BanI GGYRCC 1 cut(s) 1080
BanII GRGCYC 2 cut(s) 489, 1327
Bbv12I GWGCWC 2 cut(s) 489, 1327
BbvI GCAGC 8 cut(s) 126, 264, 429, 1207, 1210, 1213, 1216, 1222
BccI CCATC 5 cut(s) 334, 751, 812, 827, 1047
BceAI ACGGC 6 cut(s) 176, 484, 703, 736, 997, 1234
BciT130I CCWGG 2 cut(s) 1098, 1260
BcnI CCSGG 7 cut(s) 690, 906, 907, 938, 939, 978, 1299
BcoDI GTCTC 3 cut(s) 84, 818, 851
BfuAI ACCTGC 1 cut(s) 706
BglII AGATCT 1 cut(s) 129
Bme1390I CCNGG 9 cut(s) 690, 906, 907, 938, 939, 978, 1098, 1260, 1299
Bme18I GGWCC 6 cut(s) 70, 289, 1006, 1147, 1295, 1301
BmeRI GACNNNNNGTC 2 cut(s) 76, 673
BmeT110I CYCGRG 5 cut(s) 536, 905, 937, 1167, 1304
BmgBI CACGTC 2 cut(s) 259, 1159
BmgT120I GGNCC 7 cut(s) 70, 289, 649, 1006, 1147, 1295, 1301
BmrFI CCNGG 9 cut(s) 690, 906, 907, 938, 939, 978, 1098, 1260, 1299
BmrI ACTGGG 1 cut(s) 897
BmsI GCATC 3 cut(s) 459, 522, 747
BmuI ACTGGG 1 cut(s) 897
BplI GAGNNNNNCTC 2 cut(s) 14, 46
BpmI CTGGAG 1 cut(s) 430
BpuMI CCSGG 7 cut(s) 690, 906, 907, 938, 939, 978, 1299
BsaBI GATNNNNATC 1 cut(s) 795
BsaHI GRCGYC 3 cut(s) 5, 327, 731
BsaJI CCNNGG 4 cut(s) 904, 905, 906, 937
BsaWI WCCGGW 1 cut(s) 772
Bsc4I CCNNNNNNNGG 5 cut(s) 67, 626, 1097, 1103, 1139
Bse118I RCCGGY 1 cut(s) 772
Bse1I ACTGG 3 cut(s) 892, 956, 1133
Bse8I GATNNNNATC 1 cut(s) 795
BseBI CCWGG 2 cut(s) 1098, 1260
BseDI CCNNGG 4 cut(s) 904, 905, 906, 937
BseGI GGATG 1 cut(s) 743
BseJI GATNNNNATC 1 cut(s) 795
BseLI CCNNNNNNNGG 5 cut(s) 67, 626, 1097, 1103, 1139
BseMII CTCAG 1 cut(s) 258
BseNI ACTGG 3 cut(s) 892, 956, 1133
BseRI GAGGAG 2 cut(s) 359, 452
BseXI GCAGC 8 cut(s) 126, 264, 429, 1207, 1210, 1213, 1216, 1222
BsgI GTGCAG 1 cut(s) 133
Bsh1285I CGRYCG 2 cut(s) 51, 355
BshFI GGCC 2 cut(s) 650, 1132
BshNI GGYRCC 1 cut(s) 1080
BshTI ACCGGT 1 cut(s) 772
BsiEI CGRYCG 2 cut(s) 51, 355
BsiHKAI GWGCWC 2 cut(s) 489, 1327
BsiHKCI CYCGRG 5 cut(s) 536, 905, 937, 1167, 1304
BsiSI CCGG 7 cut(s) 689, 773, 906, 923, 938, 977, 1299
BslI CCNNNNNNNGG 5 cut(s) 67, 626, 1097, 1103, 1139
BsmAI GTCTC 3 cut(s) 84, 818, 851
BsmBI CGTCTC 3 cut(s) 84, 818, 851
BsmI GAATGC 2 cut(s) 1237, 1336
BsnI GGCC 2 cut(s) 650, 1132
BsoBI CYCGRG 5 cut(s) 536, 905, 937, 1167, 1304
Bsp1286I GDGCHC 2 cut(s) 489, 1327
Bsp143I GATC 7 cut(s) 85, 129, 303, 440, 564, 685, 925
Bsp68I TCGCGA 1 cut(s) 439
BspANI GGCC 2 cut(s) 650, 1132
BspCNI CTCAG 1 cut(s) 257
BspMI ACCTGC 1 cut(s) 706
BspPI GGATC 5 cut(s) 435, 680, 693, 920, 933
BspT107I GGYRCC 1 cut(s) 1080
BsrBI CCGCTC 2 cut(s) 541, 549
BsrFI RCCGGY 1 cut(s) 772
BsrI ACTGG 3 cut(s) 892, 956, 1133
BssAI RCCGGY 1 cut(s) 772
BssECI CCNNGG 4 cut(s) 904, 905, 906, 937
BssMI GATC 7 cut(s) 85, 129, 303, 440, 564, 685, 925
BssNI GRCGYC 3 cut(s) 5, 327, 731
Bst2UI CCWGG 2 cut(s) 1098, 1260
Bst4CI ACNGT 4 cut(s) 105, 265, 862, 1000
Bst6I CTCTTC 1 cut(s) 410
BstACI GRCGYC 3 cut(s) 5, 327, 731
BstAPI GCANNNNNTGC 1 cut(s) 919
BstC8I GCNNGC 6 cut(s) 143, 458, 485, 882, 898, 1193
BstDEI CTNAG 1 cut(s) 244
BstF5I GGATG 1 cut(s) 743
BstHHI GCGC 5 cut(s) 66, 201, 388, 483, 518
BstKTI GATC 7 cut(s) 88, 132, 306, 443, 567, 688, 928
BstMAI GTCTC 3 cut(s) 84, 818, 851
BstMBI GATC 7 cut(s) 85, 129, 303, 440, 564, 685, 925
BstMCI CGRYCG 2 cut(s) 51, 355
BstNI CCWGG 2 cut(s) 1098, 1260
BstSCI CCNGG 9 cut(s) 688, 904, 905, 936, 937, 976, 1096, 1258, 1297
BstV1I GCAGC 8 cut(s) 126, 264, 429, 1207, 1210, 1213, 1216, 1222
BstX2I RGATCY 3 cut(s) 129, 685, 925
BstYI RGATCY 3 cut(s) 129, 685, 925
BsuRI GGCC 2 cut(s) 650, 1132
BtgZI GCGATG 1 cut(s) 545
BtrI CACGTC 2 cut(s) 259, 1159
BtsCI GGATG 1 cut(s) 743
BtsIMutI CAGTG 1 cut(s) 1140
BtuMI TCGCGA 1 cut(s) 439
BveI ACCTGC 1 cut(s) 706
Cac8I GCNNGC 6 cut(s) 143, 458, 485, 882, 898, 1193
CfoI GCGC 5 cut(s) 66, 201, 388, 483, 518
Cfr10I RCCGGY 1 cut(s) 772
Cfr13I GGNCC 7 cut(s) 70, 289, 649, 1006, 1147, 1295, 1301
Cfr9I CCCGGG 2 cut(s) 905, 937
CseI GACGC 3 cut(s) 346, 507, 509
CsiI ACCWGGT 1 cut(s) 1096
Csp6I GTAC 6 cut(s) 101, 271, 349, 959, 1001, 1081
CspAI ACCGGT 1 cut(s) 772
CviAII CATG 3 cut(s) 109, 337, 665
CviQI GTAC 6 cut(s) 101, 271, 349, 959, 1001, 1081
DdeI CTNAG 1 cut(s) 244
DpnI GATC 7 cut(s) 87, 131, 305, 442, 566, 687, 927
DpnII GATC 7 cut(s) 85, 129, 303, 440, 564, 685, 925
DrdI GACNNNNNNGTC 1 cut(s) 326
DriI GACNNNNNGTC 2 cut(s) 76, 673
DseDI GACNNNNNNGTC 1 cut(s) 326
Eam1104I CTCTTC 1 cut(s) 410
Eam1105I GACNNNNNGTC 2 cut(s) 76, 673
EarI CTCTTC 1 cut(s) 410
EciI GGCGGA 6 cut(s) 22, 205, 383, 463, 1134, 1236
Ecl136II GAGCTC 2 cut(s) 487, 1325
Eco24I GRGCYC 2 cut(s) 489, 1327
Eco47I GGWCC 6 cut(s) 70, 289, 1006, 1147, 1295, 1301
Eco53kI GAGCTC 2 cut(s) 487, 1325
Eco88I CYCGRG 5 cut(s) 536, 905, 937, 1167, 1304
EcoICRI GAGCTC 2 cut(s) 487, 1325
EcoO109I RGGNCCY 2 cut(s) 289, 1295
EcoRII CCWGG 2 cut(s) 1096, 1258
EcoT38I GRGCYC 2 cut(s) 489, 1327
Esp3I CGTCTC 3 cut(s) 84, 818, 851
FaeI CATG 3 cut(s) 112, 340, 668
FatI CATG 3 cut(s) 108, 336, 664
FauI CCCGC 5 cut(s) 69, 150, 711, 889, 1132
FauNDI CATATG 1 cut(s) 945
FblI GTMKAC 2 cut(s) 330, 766
FokI GGATG 1 cut(s) 730
FriOI GRGCYC 2 cut(s) 489, 1327
GlaI GCGC 5 cut(s) 65, 200, 387, 482, 517
GsuI CTGGAG 1 cut(s) 430
HaeIII GGCC 2 cut(s) 650, 1132
HapII CCGG 7 cut(s) 689, 773, 906, 923, 938, 977, 1299
HgaI GACGC 3 cut(s) 346, 507, 509
HhaI GCGC 5 cut(s) 66, 201, 388, 483, 518
Hin1I GRCGYC 3 cut(s) 5, 327, 731
Hin1II CATG 3 cut(s) 112, 340, 668
Hin6I GCGC 5 cut(s) 64, 199, 386, 481, 516
HinP1I GCGC 5 cut(s) 64, 199, 386, 481, 516
HincII GTYRAC 1 cut(s) 331
HindII GTYRAC 1 cut(s) 331
HindIII AAGCTT 1 cut(s) 555
HpaII CCGG 7 cut(s) 689, 773, 906, 923, 938, 977, 1299
HphI GGTGA 5 cut(s) 277, 304, 641, 687, 787
Hpy166II GTNNAC 5 cut(s) 101, 331, 695, 767, 967
Hpy188III TCNNGA 6 cut(s) 83, 226, 438, 808, 823, 1341
Hpy8I GTNNAC 5 cut(s) 101, 331, 695, 767, 967
HpyAV CCTTC 2 cut(s) 230, 640
HpyCH4III ACNGT 4 cut(s) 105, 265, 862, 1000
HpyCH4IV ACGT 5 cut(s) 258, 327, 672, 731, 1158
HpyCH4V TGCA 5 cut(s) 114, 664, 913, 1273, 1334
HpyF3I CTNAG 1 cut(s) 244
HpySE526I ACGT 5 cut(s) 258, 327, 672, 731, 1158
Hsp92I GRCGYC 3 cut(s) 5, 327, 731
Hsp92II CATG 3 cut(s) 112, 340, 668
HspAI GCGC 5 cut(s) 64, 199, 386, 481, 516
KpnI GGTACC 1 cut(s) 1084
Kzo9I GATC 7 cut(s) 85, 129, 303, 440, 564, 685, 925
LmnI GCTCC 5 cut(s) 396, 465, 476, 554, 1330
Lsp1109I GCAGC 8 cut(s) 126, 264, 429, 1207, 1210, 1213, 1216, 1222
LweI GCATC 3 cut(s) 459, 522, 747
MabI ACCWGGT 1 cut(s) 1096
MaeII ACGT 5 cut(s) 258, 327, 672, 731, 1158
MaeIII GTNAC 5 cut(s) 6, 254, 259, 310, 629
MalI GATC 7 cut(s) 87, 131, 305, 442, 566, 687, 927
MbiI CCGCTC 2 cut(s) 541, 549
MboI GATC 7 cut(s) 85, 129, 303, 440, 564, 685, 925
MboII GAAGA 3 cut(s) 397, 552, 559
MflI RGATCY 3 cut(s) 129, 685, 925
MhlI GDGCHC 2 cut(s) 489, 1327
MluCI AATT 4 cut(s) 183, 523, 604, 931
MlyI GAGTC 6 cut(s) 23, 38, 290, 772, 1036, 1072
MmeI TCCRAC 4 cut(s) 6, 148, 579, 633
MseI TTAA 1 cut(s) 432
MspA1I CMGCKG 2 cut(s) 277, 513
MspI CCGG 7 cut(s) 689, 773, 906, 923, 938, 977, 1299
MspR9I CCNGG 9 cut(s) 690, 906, 907, 938, 939, 978, 1098, 1260, 1299
Mva1269I GAATGC 2 cut(s) 1237, 1336
MvaI CCWGG 2 cut(s) 1098, 1260
NciI CCSGG 7 cut(s) 690, 906, 907, 938, 939, 978, 1299
NdeI CATATG 1 cut(s) 945
NdeII GATC 7 cut(s) 85, 129, 303, 440, 564, 685, 925
NlaIII CATG 3 cut(s) 112, 340, 668
NmeAIII GCCGAG 1 cut(s) 786
NmuCI GTSAC 5 cut(s) 6, 254, 259, 310, 629
NruI TCGCGA 1 cut(s) 439
PaeR7I CTCGAG 1 cut(s) 536
PaqCI CACCTGC 1 cut(s) 706
PcsI WCGNNNNNNNCGW 4 cut(s) 53, 74, 291, 327
PctI GAATGC 2 cut(s) 1237, 1336
PfeI GAWTC 7 cut(s) 367, 425, 791, 811, 835, 1021, 1069
PflFI GACNNNGTC 3 cut(s) 47, 356, 733
PinAI ACCGGT 1 cut(s) 772
PleI GAGTC 6 cut(s) 23, 38, 290, 771, 1035, 1071
PpsI GAGTC 6 cut(s) 23, 38, 290, 771, 1035, 1071
PpuMI RGGWCCY 2 cut(s) 289, 1295
Psp124BI GAGCTC 2 cut(s) 489, 1327
Psp5II RGGWCCY 2 cut(s) 289, 1295
Psp6I CCWGG 2 cut(s) 1096, 1258
PspGI CCWGG 2 cut(s) 1096, 1258
PspPI GGNCC 7 cut(s) 70, 289, 649, 1006, 1147, 1295, 1301
PspPPI RGGWCCY 2 cut(s) 289, 1295
PspXI VCTCGAGB 1 cut(s) 536
PsuI RGATCY 3 cut(s) 129, 685, 925
PsyI GACNNNGTC 3 cut(s) 47, 356, 733
PvuII CAGCTG 1 cut(s) 277
RruI TCGCGA 1 cut(s) 439
RsaI GTAC 6 cut(s) 102, 272, 350, 960, 1002, 1082
RsaNI GTAC 6 cut(s) 101, 271, 349, 959, 1001, 1081
SacI GAGCTC 2 cut(s) 489, 1327
SalI GTCGAC 1 cut(s) 329
SaqAI TTAA 1 cut(s) 432
Sau3AI GATC 7 cut(s) 85, 129, 303, 440, 564, 685, 925
Sau96I GGNCC 7 cut(s) 70, 289, 649, 1006, 1147, 1295, 1301
SchI GAGTC 6 cut(s) 23, 38, 290, 772, 1036, 1072
ScrFI CCNGG 9 cut(s) 690, 906, 907, 938, 939, 978, 1098, 1260, 1299
SduI GDGCHC 2 cut(s) 489, 1327
SexAI ACCWGGT 1 cut(s) 1096
SfaNI GCATC 3 cut(s) 459, 522, 747
Sfr274I CTCGAG 1 cut(s) 536
SgrAI CRCCGGYG 1 cut(s) 772
SinI GGWCC 6 cut(s) 70, 289, 1006, 1147, 1295, 1301
SlaI CTCGAG 1 cut(s) 536
SmaI CCCGGG 2 cut(s) 907, 939
SmlI CTYRAG 1 cut(s) 536
SmoI CTYRAG 1 cut(s) 536
Sse9I AATT 4 cut(s) 183, 523, 604, 931
SstI GAGCTC 2 cut(s) 489, 1327
StyD4I CCNGG 9 cut(s) 688, 904, 905, 936, 937, 976, 1096, 1258, 1297
TaaI ACNGT 4 cut(s) 105, 265, 862, 1000
TaiI ACGT 5 cut(s) 261, 330, 675, 734, 1161
TaqII GACCGA 1 cut(s) 37
TasI AATT 4 cut(s) 183, 523, 604, 931
TauI GCSGC 4 cut(s) 38, 126, 391, 516
TfiI GAWTC 7 cut(s) 367, 425, 791, 811, 835, 1021, 1069
Tru1I TTAA 1 cut(s) 432
Tru9I TTAA 1 cut(s) 432
TscAI CASTG 1 cut(s) 1140
TseFI GTSAC 5 cut(s) 6, 254, 259, 310, 629
TseI GCWGC 8 cut(s) 114, 277, 417, 1195, 1198, 1201, 1204, 1235
Tsp45I GTSAC 5 cut(s) 6, 254, 259, 310, 629
TspDTI ATGAA 2 cut(s) 97, 1325
TspGWI ACGGA 1 cut(s) 736
TspMI CCCGGG 2 cut(s) 905, 937
TspRI CASTG 1 cut(s) 1140
Tth111I GACNNNGTC 3 cut(s) 47, 356, 733
VpaK11BI GGWCC 6 cut(s) 70, 289, 1006, 1147, 1295, 1301
XhoI CTCGAG 1 cut(s) 536
XmaI CCCGGG 2 cut(s) 905, 937
XmiI GTMKAC 2 cut(s) 330, 766
ZraI GACGTC 2 cut(s) 328, 732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.